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Journal articles on the topic 'Recombinant lines'

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Author: Grafiati

Published: 10 December 2022

Last updated: 28 January 2023

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1

Sherman, J. D., L. Y. Smith, T. K. Blake, and L. E. Talbert. "Identification of barley genome segments introgressed into wheat using PCR markers." Genome 44, no. 1 (February 1, 2001): 38–44. http://dx.doi.org/10.1139/g00-092.

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Barley has several important traits that might be used in the genetic improvement of wheat. For this report, we have produced wheat-barley recombinants involving barley chromosomes 4 (4H) and 7 (5H). Wheat-barley disomic addition lines were crossed with 'Chinese Spring' wheat carrying the ph1b mutation to promote homoeologous pairing. Selection was performed using polymerase chain reaction (PCR) markers to identify lines with the barley chromosome in the ph1b background. These lines were self pollinated, and recombinants were identified using sequence-tagged-site (STS) primer sets that allowed differentiation between barley and wheat chromosomes. Several recombinant lines were isolated that involved different STS-PCR markers. Recombination was confirmed by allowing the lines to self pollinate and rescreening the progeny via STS-PCR. Progeny testing confirmed 9 recombinants involving barley chromosome 4 (4H) and 11 recombinants involving barley chromosome 7 (5H). Some recombinants were observed cytologically to eliminate the possibility of broken chromosomes. Since transmission of the recombinant chromosomes was lower than expected and since seed set was reduced in recombinant lines, the utility of producing recombinants with this method is uncertain.Key words: introgression, sequence-tagged-site, recombination.

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2

Biagetti, Marco, Francesca Vitellozzi, and Carla Ceoloni. "Physical mapping of wheat-Aegilops longissima breakpoints in mildew-resistant recombinant lines using FISH with highly repeated and low-copy DNA probes." Genome 42, no. 5 (October 1, 1999): 1013–19. http://dx.doi.org/10.1139/g98-172.

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Fluorescence in situ hybridization (FISH) with multiple probes, consisting of highly repeated DNA sequences (pSc119.2 and pAs1) and of a low-copy, 3BS-specific RFLP sequence (PSR907), enabled determination of the physical position of the wheat-alien breakpoints (BPs) along the 3BS and 3DS arms of common wheat recombinant lines. These lines harbour 3SlS Aegilops longissima segments containing the powdery mildew resistance gene Pm13. In all 3B recombinants, the wheat-Aegilops longissima physical BPs lie within the interval separating the two most distal of the three pSc119.2 3BS sites. In all such recombinants a telomeric segment, containing the most distal of the pSc119.2 3BS sites, was in fact replaced by a homoeologous Ae. longissima segment, marked by characteristic pSc119.2 hybridization sites. Employment of the PSR907 RFLP probe as a FISH marker allowed to resolve further the critical region in the various 3B recombinant lines. Three of them, like the control common wheat, exhibited between the two most distal pSc119.2 sites a single PSR907 FISH site, which was missing in a fourth recombinant line. The amount of alien chromatin can thus be estimated to represent around 20% of the recombinant arm in the three former lines and a maximum of 27% in the latter. A similar physical length was calculated for the alien segment contained in three 3D recombinants, all characterized by the presence of the Ae. longissima pSc119.2 sites distal to the nearly telomeric pAs1 sites of normal 3DS. Comparison between the FISH-based maps and previously developed RFLP maps of the 3BS-3SlS and 3DS-3SlS arms revealed substantial differences between physical and genetic map positions of the wheat-alien BPs and of molecular markers associated with the critical chromosomal portions.Key words: wheat-alien recombinants, chromosome engineering, fluorescence in situ hybridization, highly repeated and low-copy DNA probes, physical versus genetic maps.

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3

Broman, Karl W. "The Genomes of Recombinant Inbred Lines." Genetics 169, no. 2 (November 15, 2004): 1133–46. http://dx.doi.org/10.1534/genetics.104.035212.

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4

Broman, K. W. "The Genomes of Recombinant Inbred Lines." Genetics 173, no. 4 (August 1, 2006): 2419. http://dx.doi.org/10.1093/genetics/173.4.2419.

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5

Dzianott, Aleksandra, Joanna Sztuba-Solińska, and Jozef J. Bujarski. "Mutations in the Antiviral RNAi Defense Pathway Modify Brome mosaic virus RNA Recombinant Profiles." Molecular Plant-Microbe Interactions® 25, no. 1 (January 2012): 97–106. http://dx.doi.org/10.1094/mpmi-05-11-0137.

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RNA interference (RNAi) mechanism targets viral RNA for degradation. To test whether RNAi gene products contributed to viral RNA recombination, a series of Arabidopsis thaliana RNAi-defective mutants were infected with Brome mosaic virus (BMV) RNAs that have been engineered to support crossovers within the RNA3 segment. Single-cross RNA3-RNA1, RNA3-RNA2, and RNA3-RNA3 recombinants accumulated in both the wild-type (wt) and all knock-out lines at comparable frequencies. However, a reduced accumulation of novel 3′ mosaic RNA3 recombinants was observed in ago1, dcl2, dcl4, and rdr6 lines but not in wt Col-0 or the dcl3 line. A BMV replicase mutant accumulated a low level of RNA3-RNA1 single-cross recombinants in Col-0 plants while, in a dcl2 dcl4 double mutant, the formation of both RNA3-RNA1 and mosaic recombinants was at a low level. A control infection in the cpr5-2 mutant, a more susceptible BMV Arabidopsis host, generated similar-to-Col-0 profiles of both single-cross and mosaic recombinants, indicating that recombinant profiles were, to some extent, independent of a viral replication rate. Also, the relative growth experiments revealed similar selection pressure for recombinants among the host lines. Thus, the altered recombinant RNA profiles have originated at the level of recombinant formation rather than because of altered selection. In conclusion, the viral replicase and the host RNAi gene products contribute in distinct ways to BMV RNA recombination. Our studies reveal that the antiviral RNAi mechanisms are utilized by plant RNA viruses to increase their variability, reminiscent of phenomena previously demonstrated in fungi.

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6

Taketa, Shin, Takaya Awayama, Masahiko Ichii, Makoto Sunakawa, Tomoko Kawahara, and Koji Murai. "Molecular cytogenetic identification of nullisomy 5B induced homoeologous recombination between wheat chromosome 5D and barley chromosome 5H." Genome 48, no. 1 (February 1, 2005): 115–24. http://dx.doi.org/10.1139/g04-096.

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Chromosome 5H of Hordeum vulgare 'New Golden' (NG) carries a gene(s) that accelerates heading in a wheat background. To introduce the early heading gene(s) of NG barley into the wheat genome, we attempted to induce homoeologous recombination between wheat and NG 5H chromosomes by 5B nullisomy. A nullisomic 5B, trisomic 5A, monosomic 5H plant (2n = 42) was produced from systematic crosses between aneuploid stocks of wheat group 5 chromosomes. A total of 656 F2 plants produced by self-fertilization were screened for recombinants by a PCR assay with 3 5H-specific amplicon markers. Twelve plants (1.8%) were selected as putative wheat–barley 5H recombinants. Five of them were inviable or sterile and the remaining 7 were fertile and subjected to the progeny test. Cytological analyses using fluorescence in situ hybridization and C-banding revealed that 6 of the 7 progeny lines are true homoeologous recombinants between the long arms of chromosomes 5D and 5H, but that the other one was not a recombinant having an aberrant barley telosome. The 6 cytologically confirmed recombinant lines included only 2 types (3 lines each), which were reciprocal products derived from exchanges at the same distal interval defined by two flanking markers. One type had a small 5HL segment translocated to the 5DL terminal, and the other type had a small terminal 5DL segment translocated to the 5HL terminal. In the latter type, the physical length of translocated barley segments slightly differed among lines. Homoeologous recombinants obtained in this study should be useful for further chromosome manipulation to introgress a small interstitial 5HL chromosome segment with the early heading gene(s) to wheat. Preferential occurrence of restricted types of recombinants is discussed in relation to homoeologous relationships between wheat and barley chromosomes.Key words: genomic in situ hybridization, homoeologous pairing, Hordeum vulgare, introgression, recombinant, Triticum aestivum.

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7

IPSILANDIS, C. G., and M. KOUTSIKA-SOTIRIOU. "The combining ability of recombinant S-lines developed from an F2 maize population." Journal of Agricultural Science 134, no. 2 (March 2000): 191–98. http://dx.doi.org/10.1017/s0021859699007406.

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Starting with the F2 generation of the single-cross commercial hybrid Lorena (PR3183), recombinant lines were developed combining half-sib/S1 evaluation on widely spaced plants in the direction of high yielding per se. Combining ability tests consisted of crosses between: (a) recombinant lines of common pedigree and (b) recombinant lines and freely available inbred lines. The highest-yielding crosses between recombinant lines reached 100% of the original F1 hybrid in a percentage of 14·2. Low heterosis was estimated owing to additive gene action of recombinant lines. Crosses between recombinant lines and freely available inbred lines outyielded significantly the commercial F1 hybrid in a percentage of 33·3. Heterosis was greater and the original F1 hybrid was outyielded significantly because of non-additive gene action. When the applied breeding procedure on a source population with high yield adaptability is adopted and where effects of intergenotypic competition masking the inherent genotypic value are controlled, population improvement may be substituted by combined half-sib/S1 selection for productivity of lines per se in low stress conditions during the very early stages.

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8

Mahadevappa, Mamatha, Richard A. DeScenzo, and Roger P. Wise. "Recombination of alleles conferring specific resistance to powdery mildew at the Mla locus in barley." Genome 37, no. 3 (June 1, 1994): 460–68. http://dx.doi.org/10.1139/g94-064.

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In barley (Hordeum vulgare L.), the Mla locus conditions reaction to the powdery mildew fungus Erysiphe graminis f.sp. hordei. Enrichment for genetic recombinants in the Mla region is possible by screening for recombination events between the flanking endosperm storage proteins hordeins C and B. Reciprocal crosses were made between the Franger (C.I. 16151) and Rupee (C.I. 16155) lines carrying the (Mla6 + Mla14) and Mla13 alleles, respectively. Recombinants were identified from F2 segregants by analyzing the extracted hordein polypeptides by sodium dodecyl sulphate – polyacrylamide gel electrophoresis. Two hundred and seventy-six recombinant gametes were identified from the 1800 seeds that were screened. Recombination of Mla alleles was analyzed by inoculating F4 recombinant lines with three isolates of E. graminis (A27, 5874, and CR3), which recognize specific Mla alleles. The linkage order established is Hor1–Mla6–Mla13–Mla14–Hor2. The genetic distances between Hor1–Mla6, Mla6–Mla13, and Mla13–Hor2, obtained using Mapmaker 3.0b F3 intercross analysis, are 3.9, 0.2, and 5.2 cM, respectively.Key words: recombinant, barley, powdery mildew, Mla, hordein.

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9

Kozub, N. A., I. A. Sozinov, A. Ya Bidnyk, N. A. Demianova, Ya B. Blume, and A. A. Sozinov. "Development of common wheat lines with the recombinant arm 1RS as a source of new combinations of disease and pest resistance genes." Interdepartmental Thematic Scientific Collection of Plant Protection and Quarantine, no. 62 (September 3, 2016): 143–50. http://dx.doi.org/10.36495/1606-9773.2016.62.143-150.

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A combination of recombinant-inbred lines of the F6 generation from the cross B-16 ќ AR 7086 between lines with two wheat-rye translocations, 1BL/1RS from the Petkus and 1AL/1RS from the rye Insave, was developed. Using gliadin and secalin loci as genetic markers we identified recombinant arm 1RS in positions 1A and 1B in about 10% of lines. The rest of lines with the rye material may also carry recombinant 1RS, which can be identified with DNA markers. Lines with recombinant arm 1RS may serve as a source of new combination of rye genes for disease and pest resistance.

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10

Crow, James F. "Haldane, Bailey, Taylor and Recombinant-Inbred Lines." Genetics 176, no. 2 (June 1, 2007): 729–32. http://dx.doi.org/10.1093/genetics/176.2.729.

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11

Liu, Chi-Hsien, I.-Ming Chu, and Shiaw-Min Hwang. "REFERENCE RECOMBINANT CHINESE HAMSTER OVARY CELL LINES." In Vitro Cellular & Developmental Biology - Animal 37, no. 10 (2001): 633. http://dx.doi.org/10.1290/1071-2690(2001)037<0633:rrchoc>2.0.co;2.

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12

Banks, P. M., P. J. Larkin, H. S. Bariana, E. S. Lagudah, R. Appels, P. M. Waterhouse, R. I. S. Brettell, et al. "The use of cell culture for subchromosomal introgressions of barley yellow dwarf virus resistance from Thinopyrum intermedium to wheat." Genome 38, no. 2 (April 1, 1995): 395–405. http://dx.doi.org/10.1139/g95-051.

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Barley yellow dwarf virus (BYDV) resistance has been transferred to wheat from a group 7 chromosome of Thinopyrum (Agropyron) intermedium. The source of the resistance gene was the L1 disomic addition line, which carries the 7Ai-1 chromosome. The resistance locus is on the long arm of this chromosome. BYDV resistant recombinant lines were identified after three or more generations of selection against a group 7 Th. intermedium short arm marker (red coleoptile) and selection for the presence of BYDV resistance. One recombinant line produced by ph. mutant induced homoeologous pairing and 14 recombinant lines induced by cell culture have been identified. Resistance in seven of the cell culture induced recombinants has been inherited via pollen according to Mendelian segregation ratios for up to eight generations. Meiotic analysis of heterozygotes indicates that the alien chromatin in the cell culture induced recombinants is small enough to allow regular meiotic behaviour. The ph-induced recombinant was less regular in meiosis. A probe, pEleAcc2, originally isolated from Th. elongatum and that hybridizes to dispersed repeated DNA sequences, was utilised to detect Th. intermedium chromatin, which confers resistance to BYDV, in wheat backgrounds. Quantification of these hybridization signals indicated that the translocations involved a portion of alien chromatin that was smaller than the complete long arm of 7Ai-1. Restriction fragment length polymorphism analysis confirmed the loss of the short arm of 7Ai-1 and indicated the retention of segments of the long arm of 7Ai-1. Two 7Ai-1L DNA markers always assorted with the BYDV resistance. A third 7Ai-IL DNA marker was also present in seven of eight recombinants. In all recombinants except TC7, the 7Ai-1L markers replaced the 7DL markers. None of the wheat group 7 markers was missing from TC7. It is concluded that all the resistant lines are the result of recombination with wheat chromosome 7D, except line TC7, which is the result of recombination with an unidentified nongroup 7 chromosome.Key words: Triticum, Agropyron, alien genes, translocation, somatic recombination, luteovirus.

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13

ERGEN, Nuri, and Halil TÜFEKÇİ. "Mammalian cell lines used in bioprocessing." Journal of Experimental and Clinical Medicine 39, no. 3 (August 30, 2022): 884–92. http://dx.doi.org/10.52142/omujecm.39.3.55.

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A various number of expressions and host systems are used in biologics manufacturing. The most commonly preferred systems are based on bacteria, yeast, mammalian cells, insect cells, and transgenic animals. A wide range of molecules, including insulin, mAbs, vaccines, and recombinant proteins, are produced using different host systems. Because of several reasons impacting the product quality and yield, mammalian cells are utilized. Moreover, mammalian cells are generally used in virus-based vaccine manufacturing. Chinese Hamster Ovary (CHO) is the most widely used cell line for high yield stable recombinant protein production, while Human Embryonic Kidney (HEK) is favoured for transient transfection low yield protein manufacturing, viral-based vaccine and gene and cell therapy-related vector production. Other mammalian cell lines such as NSO, Sp2.0, Vero, MRC-5 and PerC.6 are also used in both recombinant protein and virus productions. Multiple modifications are carried out on industrial cell lines to make them more suitable for high yield and high-quality protein production. Thanks to these alterations, high productivity and quality levels are achieved in the biotechnology industry.

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14

Jat, P. S., C. L. Cepko, R. C. Mulligan, and P. A. Sharp. "Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens." Molecular and Cellular Biology 6, no. 4 (April 1986): 1204–17. http://dx.doi.org/10.1128/mcb.6.4.1204-1217.1986.

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We used a murine retrovirus shuttle vector system to construct recombinants capable of constitutively expressing the simian virus 40 (SV40) large T antigen and the polyomavirus large and middle T antigens as well as resistance to G418. Subsequently, these recombinants were used to generate cell lines that produced defective helper-free retroviruses carrying each of the viral oncogenes. These recombinant retroviruses were used to analyze the role of the viral genes in transformation of rat F111 cells. Expression of the polyomavirus middle T antigen alone resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were unaltered by the criteria of morphology, anchorage-independent growth, and tumorigenicity. More surprisingly, SV40 large T-expressing cell lines were not tumorigenic despite the fact that they contained elevated levels of cellular p53 and had a high plating efficiency in soft agar. These results suggest that the SV40 large T antigen is not an acute transforming gene like the polyomavirus middle T antigen but is similar to the establishment genes such as myc and adenovirus EIa.

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15

Jat, P. S., C. L. Cepko, R. C. Mulligan, and P. A. Sharp. "Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens." Molecular and Cellular Biology 6, no. 4 (April 1986): 1204–17. http://dx.doi.org/10.1128/mcb.6.4.1204.

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We used a murine retrovirus shuttle vector system to construct recombinants capable of constitutively expressing the simian virus 40 (SV40) large T antigen and the polyomavirus large and middle T antigens as well as resistance to G418. Subsequently, these recombinants were used to generate cell lines that produced defective helper-free retroviruses carrying each of the viral oncogenes. These recombinant retroviruses were used to analyze the role of the viral genes in transformation of rat F111 cells. Expression of the polyomavirus middle T antigen alone resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were unaltered by the criteria of morphology, anchorage-independent growth, and tumorigenicity. More surprisingly, SV40 large T-expressing cell lines were not tumorigenic despite the fact that they contained elevated levels of cellular p53 and had a high plating efficiency in soft agar. These results suggest that the SV40 large T antigen is not an acute transforming gene like the polyomavirus middle T antigen but is similar to the establishment genes such as myc and adenovirus EIa.

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16

Ju, Xiaoli, Meijia Ren, Keping Chen, and Qiang Wang. "Overexpression of c-Myc enhances recombinant protein production in High Five cells after baculovirus infection." Zeitschrift für Naturforschung C 73, no. 3-4 (February 23, 2018): 147–51. http://dx.doi.org/10.1515/znc-2017-0076.

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AbstractDue to their numerous advantages, baculovirus expression vector systems (BEVS) have been widely used to express recombinant proteins for different purposes. Different strategies have been adopted to increase recombinant protein production. In this study, we transiently or stably expressed mousec-Mycin High Five cells using a commercial pIB/V5 vector. Under the control of theOpIE2promoter, this vector could enhance recombinant protein production. We found that transient expression ofc-Mycin High Five cells improved recombinant protein production. Furthermore, we established two stable cell lines, High Five-c-Myc #1 and High Five-c-Myc #2, that stably expressed mousec-Myc. We further found that the expression level of the recombinant protein was increased in these stable cell lines compared to control cell lines. These data indicate that overexpressingc-Mycin cells is a promising way to improve recombinant protein production in BEVS.

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17

Teuscher, Friedrich, and Karl W. Broman. "Haplotype Probabilities for Multiple-Strain Recombinant Inbred Lines." Genetics 175, no. 3 (December 6, 2006): 1267–74. http://dx.doi.org/10.1534/genetics.106.064063.

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18

Rockman, Matthew V., and Leonid Kruglyak. "Breeding Designs for Recombinant Inbred Advanced Intercross Lines." Genetics 179, no. 2 (May 27, 2008): 1069–78. http://dx.doi.org/10.1534/genetics.107.083873.

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19

Kumari, Pummy, Uma Ahuja, Sunita Jain, and R. K. Jain. "Fragrance Analysis among Recombinant Inbred Lines of Rice." Asian Journal of Plant Sciences 11, no. 4 (June 15, 2012): 190–94. http://dx.doi.org/10.3923/ajps.2012.190.194.

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20

Lee, Jae Seong, Helene Faustrup Kildegaard, Nathan E. Lewis, and Gyun Min Lee. "Mitigating Clonal Variation in Recombinant Mammalian Cell Lines." Trends in Biotechnology 37, no. 9 (September 2019): 931–42. http://dx.doi.org/10.1016/j.tibtech.2019.02.007.

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21

Tahir, M., and F. J. Muehlbauer. "Gene Mapping in Lentil With Recombinant Inbred Lines." Journal of Heredity 85, no. 4 (July 1994): 306–10. http://dx.doi.org/10.1093/oxfordjournals.jhered.a111464.

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22

Paran, I., I. Goldman, S. D. Tanksley, and D. Zamir. "Recombinant inbred lines for genetic mapping in tomato." Theoretical and Applied Genetics 90, no. 3-4 (March 1995): 542–48. http://dx.doi.org/10.1007/bf00222001.

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23

Schwarz, Roy D., Robert E. Davis, Juan C. Jaen, Carolyn J. Spencer, Haile Tecle, and Anthony J. Thomas. "Characterization of muscarinic agonists in recombinant cell lines." Life Sciences 52, no. 5-6 (January 1993): 465–72. http://dx.doi.org/10.1016/0024-3205(93)90303-k.

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24

Liharska, Tsvetana, Monique van Wordragen, Ab van Kammen, Pim Zabel, and Maarten Koornneef. "Tomato chromosome 6: effect of alien chromosomal segments on recombinant frequencies." Genome 39, no. 3 (June 1, 1996): 485–91. http://dx.doi.org/10.1139/g96-062.

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Variation in recombinant frequencies at two adjacent intervals on chromosome 6 of tomato (Lycopersicon esculentum Mill.) has been studied in seven lines that differ in the amount and origin of introgressed segments from wild species. These lines were all crossed to a genotype homozygous recessive for the markers tl, yv, and c, which define the centromere spanning region tl–yv and the long arm region yv–c. Recombinants were identified in large F2, populations consisting of over 30 000 plants in total. Application of molecular markers provided additional information on the distribution of crossover events within the centromere-containing interval tl–yv. A decrease in recombination at the marked intervals correlated with the presence of an alien segment. Suppression of recombination was up to sixfold in the centromere spanning interval tl–yv depending on the source and size of the introgression, and was restricted to the alien segments with no strong effect on the neighbouring intervals. Key words : recombinant frequency, Lycopersicon esculentum, morphological markers, introgressions, centromere.

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25

Sidorchuk, Yu V., A. A. Fomenkov, V. V. Kuznetsov, S. R. Mursalimov, A. A. Zagorskaya, E. A. Uvarova, P. A. Belavin, and E. V. Deineko. "Variation in GFP Gene Expression in Arabidopsis thaliana Monoclonal Cell Lines." Biotekhnologiya 35, no. 1 (2019): 58–67. http://dx.doi.org/10.21519/0234-2758-2019-35-1-58-67.

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A set of transgenic Arabidopsis thaliana monoclonal cell lines has been created by selecting individual cells (cell aggregates) with random GFP gene integration events after the Agrobacterium-mediated transformation. The yield of the recombinant GFP ranged from 0.07 to 2.37% of the total soluble protein. Three lines with the highest, about 2% of the total soluble protein, accumulation of the target GFP protein were isolated. Areas of T-DNA insertions into the plant genome for 12 monoclonal cell lines were determined. The variation in this characteristic among 21 examined cell lines can serve as a reference for A. thaliana CRISPR/Cas9 genome editing aimed at the increase in the yield of recombinant proteins in plant expression systems. The lines with the highest level of the recombinant protein accumulation are of interest for the further identification and detailed characteristics of the sites for the transgene integration. These sites can be used as targets for the gene integration during the creation of lines for the production of recombinant proteins. Arabidopsis thaliana, cell suspension culture, Agrobacterium-mediated transformation, gene expression, green fluorescent protein. The work was performed under project no. 17-14-01099 of the Russian Science Foundation.

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26

Ramsay, L. D., D. E. Jennings, M. J. Kearsey, D. F. Marshall, E. J. R. Bohuon, A. E. Arthur, and D. J. Lydiate. "The construction of a substitution library of recombinant backcross lines in Brassica oleracea for the precision mapping of quantitative trait loci." Genome 39, no. 3 (June 1, 1996): 558–67. http://dx.doi.org/10.1139/g96-071.

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The currently available methods for locating quantitative trait loci (QTLs) and measuring their effects in segregating populations lack precision unless individual QTLs have very high heritabilities. The use of recombinant backcross lines containing short regions of donor chromosome introgressed into a constant recipient background permits QTLs to be located with greater precision. The present paper describes the use of molecular markers to introgress defined short regions of chromosome from a donor doubled haploid calabrese line of Brassica oleracea (var. italica) into a recipient short generation variety (Brassica oleracea var. alboglabra). We demonstrate that in just two or three generations of backcrossing, combined with selection for mapped molecular markers, the generation of a library of recombinant backcross lines is feasible. The possible use and refinement of these lines are discussed. Key words : backcrossing, Brassica oleracea, introgression, molecular markers, near-isogenic lines, QTL mapping, recombinant backcross lines, substitution lines.

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27

Falque, M. "IRILmap: linkage map distance correction for intermated recombinant inbred lines/advanced recombinant inbred strains." Bioinformatics 21, no. 16 (June 16, 2005): 3441–42. http://dx.doi.org/10.1093/bioinformatics/bti543.

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28

Avdikos, Ilias D., Georgia-Maria Nteve, Athanasia Apostolopoulou, Rafail Tagiakas, Ioannis Mylonas, Ioannis N. Xynias, Fokion Papathanasiou, Panagiotis Kalaitzis, and Athanasios G. Mavromatis. "Analysis of Re-Heterosis for Yield and Fruit Quality in Restructured Hybrids, Generated from Crossings among Tomato Recombinant Lines." Agronomy 11, no. 5 (April 22, 2021): 822. http://dx.doi.org/10.3390/agronomy11050822.

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Randomized complete block design was used, with three replications. Heterosis for yield and fruit quality characteristics was studied, and expressed as Relative heterosis, heterobeltiosis and Standard heterosis. It would be expected, according to the dominance model, that the heterosis recorded after crossing the recombinant lines, having only a small portion of recessive deleterious alleles, would be minimal. The results showed that the elite recombinant inbred lines became the parents of elite restructured hybrids, with increased levels of re-heterosis for all characters measured. This may prove that dominance is not the only case in explaining heterosis in tomato for yield components and fruit quality characteristics. Several recombinant lines, and most of the new reconstructed F1 hybrids, showed excellent productivity under a low input farming system. The evaluation and selection of the different types of cultivars (recombinant pure lines or reconstructed hybrids) under low input conditions could point towards the most suitable/ideal genotype for organic cultivation.

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29

Ezami Razliqi, Masoud, Gholamreaza Olad, Rouhollah Dorostkar, Sahar Heydari, and Hadi Esmaeili Gouvarchin Ghaleh. "Antiproliferative Effects of Recombinant Apoptin on Lung and Breast Cancer Cell Lines." Archives of Iranian Medicine 23, no. 9 (September 1, 2020): 593–99. http://dx.doi.org/10.34172/aim.2020.69.

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Background: Selective therapy has always been the main challenge in cancer treatments. Various non-replicative oncolytic viral systems have revealed the safety and efficacy of using viruses and these products. The aim of this paper is to examine the impact of recombinant apoptin on the proliferation of lung cancer and breast cancer cell lines. Methods: The present study consisted of two steps of expression of recombinant apoptin and its anti-proliferative effects on normal and cancer cells. In the first step, following bioinformatics and optimizing apoptin gene sequencing and synthesis, it was expressed using vector PET28a and E. coli BL21 (DE3). The expressed recombinant apoptin was confirmed by analytical SDSPAGE and then purified using Ni affinity chromatography. In the second step, the antiproliferative effects of recombinant apoptin on lung cancer, breast cancer and primary cell lines were determined using MTT assay. Results: According to the results of SDS-PAGE gel assay, recombinant apoptin was visible in the 14 kDa band. Also, the MTT assay results indicated that the antiproliferative effects of recombinant apoptin in cancer cell lines was different compared with the primary cell line, and followed a dose-dependent manner in both cell lines. The highest cytotoxicity (lowest cell viability) groups were 0.2 mg/mL in lung cancer (0.32 ± 0.015) (P<0.001), and in breast cancer (0.33 ± 0.031) (P<0.001) and 0.032 mg/mL in primary cells (0.17 ± 0.004) (P<0.01), as compared to the control groups. Conclusion: Our results confirmed that recombinant apoptin can induce antiproliferative effects in lung cancer and breast cancer cell lines, but not in normal monkey kidney cell line Vero; thus, it can be introduced as a promising novel specific antitumor agent after further evaluation in clinical trials.

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Cortes, Diego Fernando Marmolejo, Renato Santa-Catarina, Alinne Oliveira Nunes Azevedo, Tathianne Pastana de Sousa Poltronieri, Julio Cesar Fiorio Vettorazzi, Nádia Fernandes Moreira, Geraldo Antônio Ferreguetti, Helaine Christine Cancela Ramos, Alexandre Pio Viana, and Messias Gonzaga Pereira. "Papaya recombinant inbred lines selection by image-based phenotyping." Scientia Agricola 75, no. 3 (May 2018): 208–15. http://dx.doi.org/10.1590/1678-992x-2016-0482.

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Martin, Olivier C., and Frédéric Hospital. "Distribution of Parental Genome Blocks in Recombinant Inbred Lines." Genetics 189, no. 2 (August 11, 2011): 645–54. http://dx.doi.org/10.1534/genetics.111.129700.

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Tsunematsu, Hiroshi, Atsushi Yoshimura, Yoshiaki Harushima, Yoshiaki Nagamura, Nori Kurata, Masahiro Yano, Takuji Sasaki, and Nobuo Iwata. "RFLP Framework Map Using Recombinant Inbred Lines in Rice." Ikushugaku zasshi 46, no. 3 (1996): 279–84. http://dx.doi.org/10.1270/jsbbs1951.46.279.

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Davis, T. R., T. J. Wickham, K. A. McKenna, R. R. Granados, M. L. Shuler, and H. A. Wood. "Comparative recombinant protein production of eight insect cell lines." In Vitro Cellular & Developmental Biology - Animal 29, no. 5 (May 1993): 388–90. http://dx.doi.org/10.1007/bf02633986.

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Maruniak, James E., Alejandra Garcia-Canedo, and Jaqueline J. S. Rodrigues. "Cell lines used for the selection of recombinant baculovirus." In Vitro Cellular & Developmental Biology - Animal 30, no. 4 (April 1994): 283–86. http://dx.doi.org/10.1007/bf02632053.

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Bulegeya, Victoria B., Mark W. Jones, Tryphone G. Muhamba, Biswanath Das, Peter R. Thomison, David M. Francis, and Margaret G. Redinbaugh. "Selecting for Coupling-Phase Recombination Between Potyvirus Resistance and White Endosperm Colour in Maize Preferred by Farmers in Sub-Saharan Africa (SSA)." Afrika Focus 32, no. 2 (February 27, 2019): 39–48. http://dx.doi.org/10.1163/2031356x-03202004.

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Maize lethal necrosis (MLN) disease caused by a combined infection of Maize chlorotic mottle virus (MCMV) and any cereal infecting potyvirus is a threat to food security in Sub-Saharan Africa (SSA). Resistance to potyvirus has been extensively studied and Mdm1 gene for potyvirus resistance on chromosome 6 of maize is linked to Y1 gene for maize endosperm colour. This study is aimed at selecting for coupling-phase recombination of potyvirus resistance and white endosperm colour. White susceptible maize lines CML333 and CML277 were crossed with a yellow resistant line, Pa405, to produce F1 and F2 progenies. Progenies were screened using molecular markers to recover 22 white endosperm recombinants. 22 selections were advanced to F3 recombinant families, and 10 were assayed for their responses to Maize dwarf mosaic virus (MDMV) and Sugarcane mosaic virus (SCMV). Four families segregated for SCMV resistance, selection of homozygous recombinants within these families will provide lines appropriate for improving lines with resistance to SCMV and MLN resistance in SSA.

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Burr, B., F. A. Burr, K. H. Thompson, M. C. Albertson, and C. W. Stuber. "Gene mapping with recombinant inbreds in maize." Genetics 118, no. 3 (March 1, 1988): 519–26. http://dx.doi.org/10.1093/genetics/118.3.519.

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Abstract Recombinant inbred lines of maize have been developed for the rapid mapping of molecular probes to chromosomal location. Two recombinant inbred families have been constructed from F2 populations of T232 X CM37 and CO159 X Tx303. A genetic map based largely on isozymes and restriction fragment length polymorphisms has been produced that covers virtually the entire maize genome. In order to map a new gene, an investigator has only to determine its allelic distribution among the recombinant inbred lines and then compare it by computer with the distributions of all previously mapped loci. The availability of the recombinant inbreds and the associated data base constitute an efficient means of mapping new molecular markers in maize.

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Ecker, Jeffrey W., Greg A. Kirchenbaum, Spencer R. Pierce, Amanda L. Skarlupka, Rodrigo B. Abreu, R. Ethan Cooper, Dawn Taylor-Mulneix, Ted M. Ross, and Giuseppe A. Sautto. "High-Yield Expression and Purification of Recombinant Influenza Virus Proteins from Stably-Transfected Mammalian Cell Lines." Vaccines 8, no. 3 (August 21, 2020): 462. http://dx.doi.org/10.3390/vaccines8030462.

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Influenza viruses infect millions of people each year, resulting in significant morbidity and mortality in the human population. Therefore, generation of a universal influenza virus vaccine is an urgent need and would greatly benefit public health. Recombinant protein technology is an established vaccine platform and has resulted in several commercially available vaccines. Herein, we describe the approach for developing stable transfected human cell lines for the expression of recombinant influenza virus hemagglutinin (HA) and recombinant influenza virus neuraminidase (NA) proteins for the purpose of in vitro and in vivo vaccine development. HA and NA are the main surface glycoproteins on influenza virions and the major antibody targets. The benefits for using recombinant proteins for in vitro and in vivo assays include the ease of use, high level of purity and the ability to scale-up production. This work provides guidelines on how to produce and purify recombinant proteins produced in mammalian cell lines through either transient transfection or generation of stable cell lines from plasmid creation through the isolation step via Immobilized Metal Affinity Chromatography (IMAC). Collectively, the establishment of this pipeline has facilitated large-scale production of recombinant HA and NA proteins to high purity and with consistent yields, including glycosylation patterns that are very similar to proteins produced in a human host.

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Hsueh, E. C., S. Knebel, I. Collier, M. Kadze, C. Hsueh, T. Lo, P. Cheng, and T. Leung. "Recombinant arginase as a novel anti-melanoma agent." Journal of Clinical Oncology 24, no. 18_suppl (June 20, 2006): 12032. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.12032.

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12032 Background: BCT-100 is a recombinant arginase comprised of 329 amino acid residues. Arginase converts arginine to urea and ornithine. Previous studies suggested that melanoma cells were auxotrophic for arginine due to absence of argininosuccinate synthetase (ASS) expression. Thus, we hypothesized that recombinant arginase, BCT-100, is cytotoxic to human melanoma cells and its cytotoxicity correlates with absence of ASS expression. Methods: BCT-100 pegylated recombinant human arginase was manufactured by large scale fermentation of a recombinant B. subtilis strain LLC101 encoded with a human arginase gene. Following fermentation, the recombinant protein was extracted, purified, pegylated, and ultra-dialyzed. Ten established human melanoma cell lines were used. Cells were grown to 90% confluence, harvested, and plated at 104 cells per well in a 96-well plate and co-cultured with increasing concentrations of pegylated BCT-100 for 72 hours. CellTiter 96 Aqueous Non-radioactive Cell Proliferation Assay (Promega, Madison, WI) was used to measure percent viability, with absorbances measured at 490 nm. Total cellular RNA was isolated from established melanoma cell lines converted to cDNA at a concentration of 5 ng/ul. Quantitative real time polymerase chain reaction was performed on a 7300 Real Time PCR System, using Gene Expression Assays for ASS and GAPDH (Applied Biosystems). 10,000 fold standard curves were generated for all samples using GAPDH expression. Results: All ten cell lines demonstrated decreased viability as concentrations of BCT-100 increased. Average IC50 value was 0.11 IU/ml. Eight of the 10 cells lines have IC50 values < 0.1 IU/ml. Of the 8 cell lines with IC50< 0.1 IU/ml, all of them have low or undetectable ASS expression using quantitative RT-PCR. Of the 2cell lines with IC50 > 0.1 IU/ml, ASS expression was detected in 1 of 2. Conclusions: Arginine depletion with recombinant arginase, BCT-100, was cytotoxic to melanoma cells in vitro. The cytotoxic effect of BCT-100 on melanoma cells correlated with expression of argininosuccinate synthetase. BCT-100 is a promising novel agent for treatment of melanoma. Further in vivo experiment with BCT-100 is ongoing. [Table: see text]

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Savolainen-Kopra, Carita, Elena Samoilovich, Heidi Kahelin, Anna-Kaisa Hiekka, Tapani Hovi, and Merja Roivainen. "Comparison of poliovirus recombinants: accumulation of point mutations provides further advantages." Journal of General Virology 90, no. 8 (August 1, 2009): 1859–68. http://dx.doi.org/10.1099/vir.0.010942-0.

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The roles of recombination and accumulation of point mutations in the origin of new poliovirus (PV) characteristics have been hypothesized, but it is not known which are essential to evolution. We studied phenotypic differences between recombinant PV strains isolated from successive stool specimens of an oral PV vaccine recipient. The studied strains included three PV2/PV1 recombinants with increasing numbers of mutations in the VP1 gene, two of the three with an amino acid change I→T in the DE-loop of VP1, their putative PV1 parent and strains Sabin 1 and 2. Growth of these viruses was examined in three cell lines: colorectal adenocarcinoma, neuroblastoma and HeLa. The main observation was a higher growth rate between 4 and 6 h post-infection of the two recombinants with the I→T substitution. All recombinants grew at a higher rate than parental strains in the exponential phase of the replication cycle. In a temperature sensitivity test, the I→T-substituted recombinants replicated equally well at an elevated temperature. Complete genome sequencing of the three recombinants revealed 12 (3), 19 (3) and 27 (3) nucleotide (amino acid) differences from Sabin. Mutations were located in regions defining attenuation, temperature sensitivity, antigenicity and the cis-acting replicating element. The recombination site was in the 5′ end of 3D. In a competition assay, the most mutated recombinant beat parental Sabin in all three cell lines, strongly suggesting that this virus has an advantage. Two independent intertypic recombinants, PV3/PV1 and PV3/PV2, also showed similar growth advantages, but they also contained several point mutations. Thus, our data defend the hypothesis that accumulation of certain advantageous mutations plays a key role in gaining increased fitness.

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Gasanov, N. B., S. V. Toshchakov, P. G. Georgiev, and O. G. Maksimenko. "The Use of Transcription Terminators to Generate Transgenic Lines of Chinese Hamster Ovary Cells (CHO) with Stable and High Level of Reporter Gene Expression." Acta Naturae 7, no. 3 (September 15, 2015): 74–80. http://dx.doi.org/10.32607/20758251-2015-7-3-74-80.

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Mammalian cell lines are widely used to produce recombinant proteins. Stable transgenic cell lines usually contain many insertions of the expression vector in one genomic region. Transcription through transgene can be one of the reasons for target gene repression after prolonged cultivation of cell lines. In the present work, we used the known transcription terminators from the SV40 virus, as well as the human - and -globin genes, to prevent transcription through transgene. The transcription terminators were shown to increase and stabilize the expression of the EGFP reporter gene in transgenic lines of Chinese hamster ovary (CHO) cells. Hence, transcription terminators can be used to create stable mammalian cells with a high and stable level of recombinant protein production.

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Yen, Yang, and P. Stephen Baenziger. "A better way to construct recombinant chromosome lines and their controls." Genome 35, no. 5 (October 1, 1992): 827–30. http://dx.doi.org/10.1139/g92-125.

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Previous procedures to construct chromosome monosomic, substitution, recombinant chromosome lines and their controls underestimated cultivar heterogeneity, which can increase background effects. Modifications are proposed to increase background homogeneity and to eliminate interference of genetic or structural heterogeneity of the target chromosomes in parental populations. To achieve this goal, a single plant or plants of single-seed descendants of disomic, monosomic, and substitution lines should be used as the parents to construct recombinant chromosome lines and the controls (i.e., the recreated disomic recurrent parent and the recreated disomic substitution line). Intracultivar variations can thus be avoided. The mating plan is carefully designed so that the resultant recombinant chromosome lines and their controls will have a similar genetic background. All the resultant lines will share the same cytoplasm and the same target chromosomes or chromosome segments that came from the parent plant(s). Therefore, we are able to largely reduce the heterogeneity in the genetic background, eliminate the potential intracultivar cytoplasm variations, and be free from the structural or genetic heterogeneity of the target chromosome. In addition, the background heterogeneity, if it exists, could be measured by comparing individual control lines.Key words: quantitative trait, cytogenetics, background heterogeneity, aneuploidy, substitution.

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Rahimi, Mohammad Hassan, Saadollah Houshmand, Mahmoud Khodambashi, Behrouz Shiran, and Shahram Mohammadi. "Evaluation of Recombinant Pure Lines of Lentil under Drought Stress." Journal of Crop Breeding 9, no. 22 (September 1, 2017): 82–97. http://dx.doi.org/10.29252/jcb.9.22.82.

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Staub, Jack E., James D. McCreight, and Juan E. Zalapa. "USDA 846-1 Fractal Melon and Derived Recombinant Inbred Lines." HortScience 46, no. 10 (October 2011): 1423–25. http://dx.doi.org/10.21273/hortsci.46.10.1423.

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Suvorova, G. N., and A. V. Ikonnikov. "Characteristics of recombinant lines of lentil L. culinaris × L. orientalis." Russian Agricultural Sciences 40, no. 1 (January 2014): 22–26. http://dx.doi.org/10.3103/s1068367414010182.

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Clark, K. Reed, Frosso Voulgaropoulou, David M. Fraley, and Philip R. Johnson. "Cell Lines for the Production of Recombinant Adeno-Associated Virus." Human Gene Therapy 6, no. 10 (October 1995): 1329–41. http://dx.doi.org/10.1089/hum.1995.6.10-1329.

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Reinhart, David, Wolfgang Sommeregger, Monika Debreczeny, Elisabeth Gludovacz, and Renate Kunert. "Characterization of recombinant IgA producing CHO cell lines by qPCR." BMC Proceedings 7, Suppl 6 (2013): P114. http://dx.doi.org/10.1186/1753-6561-7-s6-p114.

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Chandel, Uttam, BS Mankotia, and KS Thakur. "Assesment of recombinant lines of maize hybrids for inbred development." Bangladesh Journal of Botany 43, no. 3 (January 15, 2015): 363–66. http://dx.doi.org/10.3329/bjb.v43i3.21615.

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Maize (Zea mays L.) breeders currently exploit genetically narrow-base populations by deriving the recombination lines from F2 of commercial single cross hybrids. A mating design was proposed for maize hybrid evaluation as source germplasm. The commercial single cross hybrids, Hi Shell, DKC 7074 and PMZ 4, developed by the commercial company, Monsanto, were evaluated for their usefulness as germplasm. According to mating design three criteria were used: the percentage of inbreeding depression, the general combining ability and the specific combining ability. PMZ 4 had a lower percentage (21.9) of inbreeding depression, which was also combined with positive general combining ability (7.5) and negative specific combining ability. The estimated percentage of inbreeding depression was greater in DKC 7074 (31.4) and in Hi Shell (25.3). DKC 7074 also had negative general combining ability (35.5), while Hi Shell had positive specific combining ability (75.0). Therefore, evaluation through mating design showed PMZ 4 possesses more desirable genes and that its F2 may be a more profitable germplasm for developing elite inbred lines DOI: http://dx.doi.org/10.3329/bjb.v43i3.21615 Bangladesh J. Bot. 43(3): 363-366, 2014 (December)

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de Sousa Bomfim, Aline, Marcela Cristina Corrêa de Freitas, Virgínia Picanço-Castro, Mário de Abreu Soares Neto, Kamilla Swiech, Dimas Tadeu Covas, and Elisa Maria de Sousa Russo. "Human cell lines: A promising alternative for recombinant FIX production." Protein Expression and Purification 121 (May 2016): 149–56. http://dx.doi.org/10.1016/j.pep.2015.11.023.

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Keith, M. B., P. J. Farrell, K. Iatrou, and L. A. Behie. "Screening of Transformed Insect Cell Lines for Recombinant Protein Production." Biotechnology Progress 15, no. 6 (December 3, 1999): 1046–52. http://dx.doi.org/10.1021/bp990119f.

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Hacker, David L., and Sowmya Balasubramanian. "Recombinant protein production from stable mammalian cell lines and pools." Current Opinion in Structural Biology 38 (June 2016): 129–36. http://dx.doi.org/10.1016/j.sbi.2016.06.005.

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