Dissertations / Theses on the topic 'Receptor affinity'
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Scammells, Peter J., and n/a. "Pyrazolo(3,4-d)Pyrimidines and adenosine receptors: a structure/activity study." Griffith University. Division of Science and Technology, 1990. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20050826.141630.
Full textJackson, Leila J. "The dynamic regulation of the low affinity IGE receptor by toll like receptor and B cell receptor agonists /." Connect to full text via ProQuest. Limited to UCD Anschutz Medical Campus, 2008.
Find full textTypescript. Includes bibliographical references (leaves 122-129). Free to UCD Anschutz Medical Campus. Online version available via ProQuest Digital Dissertations;
Sundaramoorthy, Meena. "MODULATION OF HIGH AFFINITY HORMONE BINDING TO LH/CG RECEPTOR." UKnowledge, 2002. http://uknowledge.uky.edu/gradschool_theses/209.
Full textSmith, Lucy. "Investigating inhibitors of the IgE:high affinity receptor protein-protein interaction." Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/44210.
Full textTurner, Helen Cathryn. "Signal transduction by the high affinity receptor for immunoglobulin E, FcER1." Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301134.
Full textMueller, Thomas. "Systems for measuring B cell receptor affinity maturation in germinal centres." Thesis, University of Birmingham, 2016. http://etheses.bham.ac.uk//id/eprint/7130/.
Full textTimm, David Eugene. "Conformation, stability and interactions of the neurotrophins and the low-affinity neurotrophin receptor." Case Western Reserve University School of Graduate Studies / OhioLINK, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=case1057179020.
Full textWahlström, Niklas. "Synthesis of indoles, bisindoles and indolocarbazoles : high affinity aryl hydrocarbon receptor ligands /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-016-8/.
Full text梁頌偉 and Chung-wai Leung. "Novel gene transfer vector targeted high affinity IL-2 receptor bearing cell." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31226280.
Full textLeung, Chung-wai. "Novel gene transfer vector targeted high affinity IL-2 receptor bearing cell /." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B25248698.
Full textBruger, Annika Målin. "TCR signalling in response to affinity stimulation." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:5f9c1001-6c43-472f-a495-c9573b54a84a.
Full textNoble, April R. "Synthesis of Amphibian Alkaloids and Synthesis and Affinity of Novel Cannabinoid Receptor Ligands." ScholarWorks@UNO, 2009. http://scholarworks.uno.edu/td/1103.
Full textLi, Airong. "Variants of the #beta# subunit of the high affinity IgE receptor and atopy." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318677.
Full textKokalaki, E. K. "Affinity gradient of chimeric antigen receptor T-cells against low-antigen density target." Thesis, University College London (University of London), 2017. http://discovery.ucl.ac.uk/1563780/.
Full textDias, Ana Margarida Gonçalves Carvalho. "Development of an affinity pair “Tag-Receptor” for recombinant protein expression and purification." Master's thesis, Faculdade de Ciências e Tecnologia, 2010. http://hdl.handle.net/10362/10924.
Full textThe main objective of this work was the development of an affinity pair for the purification of recombinant proteins. In this work, ligands based on the Ugi Reaction and the 1,3,5-Triazine scaffold were synthesised in solid-phase and screened for binding to an affinity tag, an hexapeptide constituted by asparagine aminoacid (N). The ligands were tested against pure solutions of the hexapeptide and Green Fluorescence Protein (GFP), used as a model protein. The ligands that had the highest affinity for the hexapeptide and lowest affinity for the protein were selected for further studies with cellular extracts. The cellular extracts were produced in HEK 293T cells transfected with two designed vectors: one containing the GFP tagged with the affinity tag, and the other containing GFP without tag. The efficient expression of a recombinant GFP fused with the designed affinity tag was demonstrated. The cellular extracts were then loaded onto chromatographic columns containing the lead ligands immobilised onto agarose, and the amount of total protein and GFP bound and eluted noted. The results demonstrated that the Ugi ligands were less selective than the Triazine ligands for the hexapeptide. The triazine ligand 7,4 has been considered as the most selective for the designed affinity tag. In addition, preselected lead ligands for another hexapeptide (RW) of interest were studied. Mammalian cells HEK 293T were transfected with a vector expressing for GFP tagged with this peptide. The ligands immobilized onto agarose were loaded with cellular extracts, being noted that the lead A6C3 showed a high selectivity for the tag tested.
Hoffman, Crystal Joyce. "Glucocorticoid Receptor Density and Binding Affinity in Horses with Systemic Inflammatory Response Syndrome." Thesis, Virginia Tech, 2014. http://hdl.handle.net/10919/48423.
Full textMaster of Science
Taylor, Eric S. "Molecular Investigations of the Epidermal Growth Factor Receptor and Its Affinity Toward Asbestos." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1324066851.
Full textMehta, Ratnavali C. "Pharmacological evaluation of trimetoquinol analogs as affinity ligands for beta-adrenergic receptor systems /." The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487945015617968.
Full text林國泰 and Kwok-tai Lam. "Differential expression of p75 low affinity neurotrophin receptor in hypoxic-ischemic neonatal mouse brain." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B29797822.
Full textFear, David Jonathan. "Analysis of the high affinity IgE receptor (FcεRI) α-chain promoter, and its regulation." Thesis, King's College London (University of London), 1998. https://kclpure.kcl.ac.uk/portal/en/theses/analysis-of-the-high-affinity-ige-receptor-fceri-xchain-promoter-and-its-regulation(846c4e6a-5dad-40c4-aed5-4e5601944f66).html.
Full textMadian, Ashraf G. "Using affinity purification -- mass spectrometry to identify aryl hydrocarbon receptor nuclear translocator interacting proteins." Scholarly Commons, 2005. https://scholarlycommons.pacific.edu/uop_etds/605.
Full textKoide, Tomoaki. "Localization of trkB and low-affinity NGF receptor mRNA in the developing rat retina." Kyoto University, 1995. http://hdl.handle.net/2433/187158.
Full textNeuroscience Letters, vol.185(3), pp.483-186, 1995, doi:10.1016/0304-3940(95)11257-W.
Kyoto University (京都大学)
0048
新制・課程博士
博士(医学)
甲第5966号
医博第1632号
新制||医||602(附属図書館)
UT51-95-D285
京都大学大学院工学研究科脳統御医科学系専攻
(主査)教授 井出 千束, 教授 本田 孔士, 教授 菊池 晴彦
学位規則第4条第1項該当
Lam, Kwok-tai. "Differential expression of p75 low affinity neurotrophin receptor in hypoxic-ischemic neonatal mouse brain /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B21779156.
Full textLeib, Nicole [Verfasser]. "Functional cooperation of Toll-like receptor signaling and the high-affinity receptor for IgE, FcεRI, on human Langerhans cells / Nicole Leib." Bonn : Universitäts- und Landesbibliothek Bonn, 2018. http://d-nb.info/1153467194/34.
Full textMarsh, Andrew. "Characterisation of the type I IGF receptor binding surfaces of insulin-like growth factor 1 using protein engineering." Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245705.
Full textBonnefoy, Jean-Yves. "The low affinity receptor for IgE on human B lymphocytes : detection, biochemical characterization and regulation." Lyon 1, 1987. http://www.theses.fr/1987LYO1T147.
Full textHoshikawa, Hajime. "High affinity binding of oxidized LDL to mouse lectin-like oxidized LDL receptor (LOX-1)." Kyoto University, 1999. http://hdl.handle.net/2433/182282.
Full textLiang, Boying. "Studies of Ligand-Receptor Pairs Utilizing Polymerized Planar Supported Lipid Bilayers." Diss., The University of Arizona, 2013. http://hdl.handle.net/10150/311232.
Full textSharma, Kanika [Verfasser], and Sigrun [Gutachter] Korsching. "Olfactory coding in vertebrates: a novel tuning mechanism for receptor affinity and evolution of the olfactory receptor repertoire / Kanika Sharma ; Gutachter: Sigrun Korsching." Köln : Universitäts- und Stadtbibliothek Köln, 2018. http://d-nb.info/1204199361/34.
Full textShu, Hong. "Design and Synthesis of CB1 Receptor Ligands and Synthesis of Amphibian Alkaloids." ScholarWorks@UNO, 2009. http://scholarworks.uno.edu/td/1104.
Full textRedhu, Naresh Singh. "Molecular regulation and effector functions of the high affinity IgE receptor (FcεRI) in human airway smooth muscle cells." PLoS One (The Public Library of Science, USA), 2009. http://hdl.handle.net/1993/5305.
Full textKhan, Samina E. "An investigation of structure activity effects of D-ring substitution of estradiol on estrogen receptor affinity." Thesis, Loughborough University, 1992. https://dspace.lboro.ac.uk/2134/28192.
Full textSavdie, Cheryl. "Characterization of the cellular and molecular determinants for the internalization of the high affinity neurotensin receptor." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=29473.
Full textIn this study, the internalization of the NTR1 was investigated. (Abstract shortened by UMI.)
Linhult, Martin. "Protein engineering to explore and improve affinity ligands." Doctoral thesis, KTH, Biotechnology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3632.
Full textIn order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. They are composed of repetitive units in whicheach one binds the ligand independently. The domains foldindependently and are very stable. Since the domains also havewellknown three-dimensional structures and do not containcysteine residues, they are very suitable as frameworks forfurther protein engineering.
Streptococcal protein G (SPG) is a multidomain proteinpresent on the cell surface ofStreptococcus. X-ray crystallography has been used todetermine the binding site of the Ig-binding domain. In thisthesis the region responsible for the HSA affinity of ABD3 hasbeen determined by directed mutagenesis followed by functionaland structural analysis. The analysis shows that the HSAbindinginvolves residues mainly in the second α-helix.
Most protein-based affinity chromatography media are verysensitive towards alkaline treatment, which is the preferredmethod for regeneration and removal of contaminants from thepurification devices in industrial applications. Here, aprotein engineering strategy has been used to improve thetolerance to alkaline conditions of different domains fromprotein G, ABD3 and C2. Amino acids known to be susceptibletowards high pH were substituted for less alkali susceptibleresidues. The new, engineered variants of C2 and ABD shownhigher stability towards alkaline pH. Also, very important forthe potential use as affinity ligands, these mutated variantsretained the secondary structure and the affinity to HSA andIgG, respectively. Moreover, dimerization was performed toinvestigate whether a higher binding capacity could be obtainedby multivalency. For ABD, binding studies showed that divalentligands coupled using non-directed chemistry demonstrated anincreased molar binding capacity compared to monovalentligands. In contrast, equal molar binding capacities wereobserved for both types of ligands when using a directed ligandcoupling chemistry involving the introduction and recruitmentof a unique C-terminal cysteine residue.
The staphylococcal protein A-derived domain Z is also a wellknown and thoroughly characterized fusion partner widely usedin affinity chromatography systems. This domain is consideredto be relatively tolerant towards alkaline conditions.Nevertheless, it is desirable to further improve the stabilityin order to enable an SPA-based affinity medium to withstandeven longer exposure to the harsh conditions associated withcleaning in place (CIP) procedures. For this purpose adifferent protein engineering strategy was employed. Smallchanges in stability due to the mutations would be difficult toassess. Hence, in order to enable detection of improvementsregarding the alkaline resistance of the Z domain, a by-passmutagenesis strategy was utilized, where a mutated structurallydestabilized variant, Z(F30A) was used as a surrogateframework. All eight asparagines in the domain were exchangedone-by-one. The residues were all shown to have differentimpact on the alkaline tolerance of the domain. By exchangingasparagine 23 for a threonine we were able to remarkablyincrease the stability of the Z(F30A)-domain towards alkalineconditions. Also, when grafting the N23T mutation to the Zscaffold we were able to detect an increased tolerance towardsalkaline treatment compared to the native Z molecule. In allcases, the most sensitive asparagines were found to be locatedin the loops region.
In summary, the work presented in this thesis shows theusefulness of protein engineering strategies, both to explorethe importance of different amino acids regarding stability andfunctionality and to improve the characteristics of aprotein.
Keywords:binding, affinity, human serum albumin (HSA),albumin-binding domain (ABD), affinity chromatography,deamidation, protein A, stabilization, Z-domain, capacity,protein G, cleaning-in-place (CIP), protein engineering, C2receptor.
Sato, Sumito. "High-affinity urokinase-derived cyclic peptides inhibiting urokinase-urokinase receptor-interaction : effects on tumor growth and spread." kostenfrei, 2009. https://mediatum2.ub.tum.de/node?id=653320.
Full textShenderov, Eugene. "In vivo and In vitro Studies of T-Cell Receptor-ligland-MHC Affinity and T-Cell Function." Thesis, University of Oxford, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.504593.
Full textKramer, A. M. "Delineating the impact of binding-domain affinity and kinetic properties on Chimeric Antigen Receptor T-cell function." Thesis, University College London (University of London), 2017. http://discovery.ucl.ac.uk/1558284/.
Full textFournet, Steven P. "High Resolution X-ray Diffraction Analysis of CB1 Receptor Antagonists as a Means to Explore Binding Affinity." ScholarWorks@UNO, 2013. http://scholarworks.uno.edu/td/1737.
Full textPaul, Alan Burnett. "β-NGF and its low-affinity receptor (p75LNGFR) in benign prostatic hyperplasia and adenocarcinoma of the prostate." Thesis, University of Edinburgh, 1999. http://hdl.handle.net/1842/29936.
Full textMockford, Esther Helen. "The identification and expression of a novel variant of the beta subunit of the high affinity IgE receptor." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312622.
Full textMcCarthy-Troke, Melanie. "Comparative modelling and mutational analysis of the alpha-subunit of the high affinity receptor for IgG, FcγRI." Thesis, University of Glasgow, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.399675.
Full textZloh, Mire. "Conformational studies of the domains and subunits of the high affinity IgE receptor by NMR and molecular modelling." Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312946.
Full textPardoe, David Alan. "Synthetic chemistry and protein molecular modelling towards novel, high affinity ligands for the 5-HTâ†1â†E receptor." Thesis, University of Bristol, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388103.
Full textFriedman, Mikaela. "Affibody molecules targeting the epidermal growth factor receptor for tumor imaging applications." Doctoral thesis, Stockholm : School of Biotechnology, Royal Institute of Technology (KTH), 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4710.
Full textKrauchunas, Amber R. M. "CD8⁺ T-cell response potential, as determined by expression of the high affinity interleukin-2 receptor, in murine AIDS /." Connect to online version, 2006. http://ada.mtholyoke.edu/setr/websrc/pdfs/www/2006/149.pdf.
Full textFerri, Catharine C. "The role of the p75 low-affinity neurotrophin receptor in the peripheral and central nervous systems following nerve injury." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ31203.pdf.
Full textSihra, Bhupinder Singh. "Studies investigating peripheral blood derived cells that express the high affinity receptor for immunoglobulin E (FceRI) in allergic disorders." Thesis, University of Southampton, 2008. https://eprints.soton.ac.uk/67619/.
Full textSarpong, Yaw Acheampong. "Influence of HMGB1 on Nucleosome Structure and Estrogen Receptor Binding Affinity to Concensus Estrogen Response Element on Nucleosomal DNA." Bowling Green State University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1288629979.
Full textSARPONG, YAW A. "THE BINDING OF ESTROGEN, PROGESTERONE AND GLUCOCORTICOID RECEPTORS TO THEIR RECOGNITION SITES IN A NUCLEOSOME AND THE EFFECT OF HMGB1 ON THE BINDING AFFINITY." Bowling Green State University / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1154368368.
Full textWatson, Diana Ruth. "The role of ASP 195 in the transmembrane domain of the #alpha# subunit of the high affinity receptor for IgE." Thesis, University of Sheffield, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312290.
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