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Relevant bibliographies by topics / Reassembly

Academic literature on the topic 'Reassembly'

Author: Grafiati

Published: 4 June 2021

Last updated: 20 February 2023

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Journal articles on the topic "Reassembly"

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Wang, Haiping, Yufu Zang, Fuxun Liang, Zhen Dong, Hongchao Fan, and Bisheng Yang. "A Probabilistic Method for Fractured Cultural Relics Automatic Reassembly." Journal on Computing and Cultural Heritage 14, no. 1 (February 2021): 1–25. http://dx.doi.org/10.1145/3417711.

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Masses of fragile cultural relics are dug out in fragments due to long-standing burying and their fragility, which must be reassembled to play a role in cultural heritage study. However, it is very challenging to automatically reassemble a large collection of fragments of unknown geometric shapes. In this article, a novel probabilistic method for fractured cultural relics automatic reassembly is proposed to solve the problem in terms of good accuracy, efficiency, and robustness. First, a set of matching units are detected and described by the 2D Link-Chain Descriptors (LCD) and the 3D Spatial-Distribution Descriptors (SDD). Second, the pairwise reassembly probability is calculated by combining the similarities of LCD and SDD descriptors, then the collision detection is conducted to eliminate the incorrect overlapping pairs. Finally, a global optimal reassembly solution is obtained by iterative graph optimization with the constrains of the loop-closures and overlap restrictions. Comprehensive experiments with eight challenging datasets demonstrate that the proposed method achieved good performance in terms of minor reassembly errors, efficiency and robustness to noise, varying point density and completeness.

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Gil, Kook-Hee, and Marsden Heather. "Existential quantifiers in second language acquisition." Linguistic Approaches to Bilingualism 3, no. 2 (May 17, 2013): 117–49. http://dx.doi.org/10.1075/lab.3.2.01gil.

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Lardiere’s (2005, 2008, 2009) Feature Reassembly Hypothesis proposes that L2 acquisition involves reconfiguring the sets of lexical features that occur in the native language into feature bundles appropriate to the L2. This paper applies the Feature Reassembly Hypothesis to findings from recent research into the L2 acquisition of existential quantifiers. It firstly provides a feature-based, crosslinguistic account of polarity item any in English, and its equivalents — wh-existentials — in Chinese, Korean and Japanese. We then test predictions built on the Feature Reassembly Hypothesis, about how learners map target existential quantifiers in the L2 input onto feature sets from their L1, and how they then reassemble these feature sets to better match the target. The findings, which are largely compatible with the predictions, show that research that focuses on the specific processes of first mapping and then feature reassembly promises to lead to a more explanatory account of development in L2 acquisition.

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Imamura, Hiroshi, Kenji Takaishi, Katsutoshi Nakano, Atsuko Kodama, Hideto Oishi, Hitoshi Shiozaki, Morito Monden, Takuya Sasaki, and Yoshimi Takai. "Rho and Rab Small G Proteins Coordinately Reorganize Stress Fibers and Focal Adhesions in MDCK Cells." Molecular Biology of the Cell 9, no. 9 (September 1998): 2561–75. http://dx.doi.org/10.1091/mbc.9.9.2561.

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The Rho subfamily of the Rho small G protein family (Rho) regulates formation of stress fibers and focal adhesions in many types of cultured cells. In moving cells, dynamic and coordinate disassembly and reassembly of stress fibers and focal adhesions are observed, but the precise mechanisms in the regulation of these processes are poorly understood. We previously showed that 12-O-tetradecanoylphorbol-13-acetate (TPA) first induced disassembly of stress fibers and focal adhesions followed by their reassembly in MDCK cells. The reassembled stress fibers showed radial-like morphology that was apparently different from the original. We analyzed here the mechanisms of these TPA-induced processes. Rho inactivation and activation were necessary for the TPA-induced disassembly and reassembly, respectively, of stress fibers and focal adhesions. Both inactivation and activation of the Rac subfamily of the Rho family (Rac) inhibited the TPA-induced reassembly of stress fibers and focal adhesions but not their TPA-induced disassembly. Moreover, microinjection or transient expression of Rab GDI, a regulator of all the Rab small G protein family members, inhibited the TPA-induced reassembly of stress fibers and focal adhesions but not their TPA-induced disassembly, indicating that, furthermore, activation of some Rab family members is necessary for their TPA-induced reassembly. Of the Rab family members, at least Rab5 activation was necessary for the TPA-induced reassembly of stress fibers and focal adhesions. The TPA-induced, small G protein-mediated reorganization of stress fibers and focal adhesions was closely related to the TPA-induced cell motility. These results indicate that the Rho and Rab family members coordinately regulate the TPA-induced reorganization of stress fibers and focal adhesions that may cause cell motility.

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Sehgal, Pravin B., Huijuan Yuan, and Ye Jin. "Rapid Reversible Osmoregulation of Cytoplasmic Biomolecular Condensates of Human Interferon-α-Induced Antiviral MxA GTPase." International Journal of Molecular Sciences 23, no. 21 (October 22, 2022): 12739. http://dx.doi.org/10.3390/ijms232112739.

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We previously discovered that exogenously expressed GFP-tagged cytoplasmic human myxovirus resistance protein (MxA), a major antiviral effector of Type I and III interferons (IFNs) against several RNA- and DNA-containing viruses, existed in the cytoplasm in phase-separated membraneless biomolecular condensates of varying sizes and shapes with osmotically regulated disassembly and reassembly. In this study we investigated whether cytoplasmic IFN-α-induced endogenous human MxA structures were also biomolecular condensates, displayed hypotonic osmoregulation and the mechanisms involved. Both IFN-α-induced endogenous MxA and exogenously expressed GFP-MxA formed cytoplasmic condensates in A549 lung and Huh7 hepatoma cells which rapidly disassembled within 1–2 min when cells were exposed to 1,6-hexanediol or to hypotonic buffer (~40–50 mOsm). Both reassembled into new structures within 1–2 min of shifting cells to isotonic culture medium (~330 mOsm). Strikingly, MxA condensates in cells continuously exposed to culture medium of moderate hypotonicity (in the range one-fourth, one-third or one-half isotonicity; range 90–175 mOsm) first rapidly disassembled within 1–3 min, and then, in most cells, spontaneously reassembled 7–15 min later into new structures. This spontaneous reassembly was inhibited by 2-deoxyglucose (thus, was ATP-dependent) and by dynasore (thus, required membrane internalization). Indeed, condensate reassembly was preceded by crowding of the cytosolic space by large vacuole-like dilations (VLDs) derived from internalized plasma membrane. Remarkably, the antiviral activity of GFP-MxA against vesicular stomatitis virus survived hypoosmolar disassembly and subsequent reassembly. The data highlight the exquisite osmosensitivity of MxA condensates, and the preservation of antiviral activity in the face of hypotonic stress.

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Wang, Xiaojun, and Brendan Cronin. "TCP/IP Reassembly in Network Intrusion Detection and Prevention Systems." International Journal of Information Security and Privacy 8, no. 3 (July 2014): 63–76. http://dx.doi.org/10.4018/ijisp.2014070104.

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Deep Packet Inspection (DPI) in Network Intrusion Detection and Prevention Systems (NIDPS) typically involves the matching of packet payloads against attack signatures in the form of fixed strings and regular expressions. As an attack pattern may span multiple IP fragments or TCP segments, accurate DPI requires that the traffic is reassembled prior to analysis of the payload data stream. Although hardware acceleration of the TCP layer, including reassembly, is well known in the form of TCP Offload Engines for Network Interface Cards, only limited research has been conducted into reassembly architectures suited to the particular requirements of DPI systems. The challenging requirements include the tracking and fragment/segment reordering of a potentially very large number of streams in addition to dealing with subtle ambiguities in IP fragmentation and TCP segmentation using target based reassembly or traffic normalization. In this article, the authors present a combined hardware and software architecture which harnesses the resources of the latest FPGA technology to improve on existing research proposals.

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McCarthy, Michael P., Wendy I. White, Frances Palmer-Hill, Scott Koenig, and Joann A. Suzich. "Quantitative Disassembly and Reassembly of Human Papillomavirus Type 11 Viruslike Particles In Vitro." Journal of Virology 72, no. 1 (January 1, 1998): 32–41. http://dx.doi.org/10.1128/jvi.72.1.32-41.1998.

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ABSTRACT The human papillomavirus (HPV) capsid is primarily composed of a structural protein denoted L1, which forms both pentameric capsomeres and capsids composed of 72 capsomeres. The L1 protein alone is capable of self-assembly in vivo into capsidlike structures referred to as viruslike particles (VLPs). We have determined conditions for the quantitative disassembly of purified HPV-11 L1 VLPs to the level of capsomeres, demonstrating that disulfide bonds alone are essential to maintaining long-term HPV-11 L1 VLP structure at physiological ionic strength. The ionic strength of the disassembly reaction was also important, as increased NaCl concentrations inhibited disassembly. Conversely, chelation of cations had no effect on disassembly. Quantitative reassembly to a homogeneous population of 55-nm, 150S VLPs was reliably achieved by the re-formation of disulfide linkages following removal of reducing agent at near-neutral pH and moderate NaCl concentration. HPV-11 L1 VLPs could also be dissociated by treatment with carbonate buffer at pH 9.6, but VLPs could not be regenerated following carbonate treatment. When probed with conformationally sensitive and/or neutralizing monoclonal antibodies, both capsomeres generated by disulfide reduction of purified VLPs and reassembled VLPs formed from capsomeres upon removal of reducing agents exhibited epitopes found on the surface of authentic HPV-11 virions. Antisera raised against either purified VLP starting material or reassembled VLPs similarly neutralized infectious HPV-11 virions. The ability to disassemble and reassemble VLPs in vitro and in bulk allows basic features of capsid assembly to be studied and also opens the possibility of packaging selected exogenous compounds within the reassembled VLPs.

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Tachikawa, Masashi, and Atsushi Mochizuki. "Golgi apparatus self-organizes into the characteristic shape via postmitotic reassembly dynamics." Proceedings of the National Academy of Sciences 114, no. 20 (May 1, 2017): 5177–82. http://dx.doi.org/10.1073/pnas.1619264114.

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The Golgi apparatus is a membrane-bounded organelle with the characteristic shape of a series of stacked flat cisternae. During mitosis in mammalian cells, the Golgi apparatus is once fragmented into small vesicles and then reassembled to form the characteristic shape again in each daughter cell. The mechanism and details of the reassembly process remain elusive. Here, by the physical simulation of a coarse-grained membrane model, we reconstructed the three-dimensional morphological dynamics of the Golgi reassembly process. Considering the stability of the interphase Golgi shape, we introduce two hypothetical mechanisms—the Golgi rim stabilizer protein and curvature-dependent restriction on membrane fusion—into the general biomembrane model. We show that the characteristic Golgi shape is spontaneously organized from the assembly of vesicles by proper tuning of the two additional mechanisms, i.e., the Golgi reassembly process is modeled as self-organization. We also demonstrate that the fine Golgi shape forms via a balance of three reaction speeds: vesicle aggregation, membrane fusion, and shape relaxation. Moreover, the membrane fusion activity decreases thickness and the number of stacked cisternae of the emerging shapes.

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LeGette, Casie. "Reassembly: Introduction." Nineteenth Century Studies 30 (January 1, 2018): 1–5. http://dx.doi.org/10.5325/ninecentstud.30.2017-18.0001.

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Souter, E., M. Pypaert, and G. Warren. "The Golgi stack reassembles during telophase before arrival of proteins transported from the endoplasmic reticulum." Journal of Cell Biology 122, no. 3 (August 1, 1993): 533–40. http://dx.doi.org/10.1083/jcb.122.3.533.

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HeLa cells arrested in prometaphase were pulse-labeled with [35S]methionine and chased in the absence of nocodazole to allow passage through mitosis and into G1. Transport of histocompatibility antigen (HLA) molecules to the medial- and trans-Golgi cisternae was measured by monitoring the resistance to endoglycosidase H and the acquisition of sialic acid residues, respectively. Transport to the plasma membrane was measured using neuraminidase to remove sialic acid residues on surface HLA molecules. The half-time for transport to each of these compartments was about 65-min longer in cells progressing out of mitosis than in G1 cells. This delay was only 5-min longer than the half-time for the fall in histone H1 kinase activity suggesting that inactivation of the mitotic kinase triggers the resumption of protein transport. The half-time for reassembly of the Golgi stack, measured using stereological procedures, was also 65 min, suggesting that both transport and reassembly are triggered at the same time. However, since reassembly was complete within 5 min, whereas HLA took 25 min to reach the medial-cisterna, we can conclude that the Golgi stack has reassembled by the time HLA reaches it.

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Romei, Matthew G., and Steven G. Boxer. "Split Green Fluorescent Proteins: Scope, Limitations, and Outlook." Annual Review of Biophysics 48, no. 1 (May 6, 2019): 19–44. http://dx.doi.org/10.1146/annurev-biophys-051013-022846.

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Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein–protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies.

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More sources

Dissertations / Theses on the topic "Reassembly"

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Onischenko, Evgeny. "Disassembly and reassembly of the nuclear pore complex /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-929-7/.

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Schermer, Ulrike. "Mechanism of chromatin reassembly at the yeast PHO5 promoter upon repression." Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-64228.

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Shimanskaya, Elena Mikhaylovna. "Feature reassembly of semantic and morphosyntactic pronominal features in L2 acquisition." Diss., University of Iowa, 2015. https://ir.uiowa.edu/etd/1902.

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Previous research in Second Language Acquisition (SLA) has shown that some of the systematic errors of second language (L2) learners can be attributed to the influence of the native language (L1). In fact, many hypotheses in generative SLA have focused on the role of L1 transfer ranging the spectrum from No Transfer to Full Transfer. The goal of this dissertation was to investigate L1 transfer by focusing on L1-L2 differences in terms of linguistic features; specifically, how differences in the featural and morpholexical organization of L1 and L2 pronominal paradigms affect SLA. In this work I operationalize L1 transfer in terms of the Feature Reassembly Hypothesis (FRH; Lardiere, 2009). The hypothesis pioneers conceptualization of L1 transfer as an initial attempt by L2 learners to establish a direct mapping between L1 and L2 forms. The FRH is particularly suitable to the study of L2 development because it predicts that when a one-to-one initial mapping is unsuccessful, L2 learners will gradually reorganize the L1 grammatical system until they attain (possibly complete) convergence. Empirical testing of the hypothesis is critical since determining when and why transfer occurs opens numerous possibilities to predict transfer errors and to develop pedagogical approaches to tackle negative transfer. In the current study I focus on the L2 acquisition of four 3rd person singular French object pronouns in the interlanguage of native speakers of English. Difficulties in the acquisition of Romance object pronouns have been amply documented in L2 research. However, most of the previous studies of the topic have focused on L2 acquisition of clitic pronouns and their syntactic properties. The present study takes a novel approach investigating the acquisition of strong as well as clitic pronouns. In my dissertation I test different kinds of knowledge including learners' comprehension of different kinds of pronouns. Going beyond production data, my experimental tasks include a grammaticality judgment task with correction, a picture selection task, and a self-paced reading task. The experimental tasks were administered to a group of native speakers (n=43) and L2 learners of French (n=87). The overall picture that emerges from the current study allows unveiling the initial mapping and subsequent reassembly of the semantic and morphosyntactic features implicated in the acquisition process of the four forms under investigation.

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Kumas, Gozde. "Detecting G-protein Coupled Receptor Interactions Using Enhanced Green Fluorescent Protein Reassembly." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614136/index.pdf.

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The largest class of cell surface receptors in mammalian genomes is the superfamily of G protein-coupled receptors (GPCRs) which are activated by a wide range of extracellular responses such as hormones, pheromones, odorants, and neurotransmitters. Drugs which have therapeutic effects on a wide range of diseases are act on GPCRs. In contrast to traditional idea, it is recently getting accepted that G-protein coupled receptors can form homo- and hetero-dimers and this interaction could have important role on maturation, internalization, function or/and pharmacology. Bimolecular fluorescence complementation technique (BiFC)
is an innovative approach based on the reassembly of protein fragments which directly report interactions. In our study we implemented this technique for detecting and visualizing the GPCR interactions in yeast cells. The enhanced green fluorescent protein (EGFP) fractionated into two fragments at genetic level which does not possess fluorescent function. The target proteins which are going to be tested in terms of interaction are modified with the non-functional fragments, to produce the fusion proteins. The interaction between two target proteins, in this study Ste2p receptors which are alpha pheromone receptors from Saccharomyces cerevisiae, enable the fragments to come in a close proximity and reassemble. After reassembly, EGFP regains its fluorescent function which provides a direct read-out for the detection of interaction. Further studies are required to determine subcellular localization of the interaction. Moreover, by using the fusion protein partners constructed in this study, effects of agonist/antagonist binding and post-translational modifications such as glycosylation and phosphorylation can be examined. Apart from all, optimized conditions for BiFC technique will guide for revealing new protein-protein interactions.

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Kennan, Mary Anne, and Fletcher T. H. Cole. "Institutional repositories as portents of change: Disruption or reassembly? Conjectures and reconfigurations." Richard B. Hill, 2008. http://hdl.handle.net/10150/105838.

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This paper reviews how Open Access policies (OA) and Institutional Repositories (IR) might be portrayed as agents of change within the realm of scholarly publishing. Using commentary on academic publishing as background, commentary that sees OA and IR as optimal and inevitable, and beneficially disruptive of the existing system, two theoretical approaches are presented as ways of providing a more detailed and explicit analysis of OA/IR dynamics. Both theories to varying degrees derive their inspiration from an exploration of the nature of change. The first â disruptive technology/disruptive innovationâ approach (Christensen) specifies change in market theory terms, a re-structuring "driven" by innovation within, and possibly disruptive of, existing market arrangements. The second approach views change as a process of "reassembling" and reconfiguring of relationships between elements of a network (Actor-Network Theory). The application of both approaches to OA/IR is explored, including reference to a case study on a university institutional repository implementation. While "disruption" and similar terms might be in common and casual use, the basic idea gains greater clarity in these theories, and in doing so promotes greater awareness of the assumptions being made, and the aspirations being pursued.

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Yarashus, Heather R. "Stripping and Reassembly of the Yeast Vacuolar H+-ATPase Peripheral Protein Subunits." W&M ScholarWorks, 1993. https://scholarworks.wm.edu/etd/1539625807.

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Mendes, Luis Felipe Santos. "Structural and dynamic characterization of the Golgi Reassembly and Stacking Protein (GRASP) in solution." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/59/59135/tde-18042018-094959/.

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The Golgi complex is an organelle responsible for receiving synthesized cargo from the endoplasmic reticulum for subsequent post-translations modifications, sorting and secretion. A family of proteins named Golgi Reassembly and Stacking Proteins (GRASP) is essential for the correct assembly and laterally tethering of the Golgi cisternae, a necessary structuration to keep this organelle working correctly. The GRASP structure is mainly composed of two regions: an N-terminal formed by two PDZ domains connected by a short loop (GRASP domain) and a non-conserved C-terminal region, rich in serine and proline residues. Although there are now a few crystal structures solved for the N-terminal domain, it is surprising to notice that no information is currently available regarding a full-length protein or even about dynamic and structural differences between the two PDZs in solution, which is the main functional region of this protein. Using a full-length GRASP model, we were capable of detecting the coexistence of regular secondary structures and large amounts of disordered regions. The overall structure is less compact than a regular globular protein and the high structural flexibility makes its hydrophobic core more accessible to solvent. GRASP coexist in a dynamic conformational ensemble of a µs-ms timescale. Our results indicate an unusual behavior of GRASP in solution, closely resembling a class of collapsed intrinsically disordered proteins called molten globule. We report here also the disorder-to-order transition propensities for a native molten globule-like protein in the presence of different mimetics of cell conditions. Changes in the dielectric constant (such as those experienced close to the membrane surface) seem to be the major factor capable of inducing several disorder-to-order transitions in GRASP, which seems to show very distinct behavior when in conditions that mimic the vicinity of the membrane surface as compared to those found when free in solution. Other folding factors such as molecular crowding, counter ions, pH and phosphorylation exhibit lower or no effect on GRASP secondary structure and/or stability. This is the first study focusing on understanding the disorder-to-order transitions of a molten globule structure without the need for any mild denaturing condition. Regarding the PDZs that form the GRASP domain, we observed that GRASPs are formed by a more unstable and flexible PDZ1 and much more stable and structurally well-behaved PDZ2. More than that, many of the unstable regions found in PDZ1 are in the predicted binding pocket, suggesting a structural promiscuity inside this domain that correlates with the functional promiscuity of interacting with multiple protein partners. This thesis presents the first structural characterization of a full-length GRASP, the first model of how GRASPs (or any molten globule-like protein) can be modulated by the cell during different cell functionalities and the first work in the community proving that the established idea that both PDZs are structurally equivalent is not completely right
O complexo de Golgi é um organela responsável pela recepção de carga sintetizada no retículo endoplasmático e por subsequente modificações pós-traducionais, classificação e secreção. Uma família de proteínas chamada Golgi Reassembly and Stacking Proteins (GRASP) é essencial para o correto empilhamento das cisternas e conexões laterais das pilhas do complexo de Golgi, uma estruturação necessária para manter essa organela funcionando corretamente. A estrutura das GRASPs é composta de duas regiões principais: uma extensão N-terminal formado por dois domínios PDZ conectados por um loop (domínio GRASP) e uma região C-terminal não conservada, rica em resíduos de serina e prolina. Embora existam algumas estruturas cristalográficas resolvidas para o domínio N-terminal, é surpreendente notar que não havia nenhuma informação na literatura sobre a construção inteira de um GRASP, ou mesmo um estudo detalhado sobre os PDZs no N-terminal em solução, que é a principal região funcional dessa proteína. Usando um modelo de GRASP em sua construção completa, fomos capazes de detectar a coexistência de estruturas secundárias regulares e grandes quantidades de regiões desordenadas. A estrutura é menos compacta do que uma proteína globular e a alta flexibilidade estrutural torna o seu núcleo hidrofóbico mais acessível ao solvente. GRASPs coexistem em um conjunto conformacional dinâmico numa escala de tempo característico de s-ms. Nossos resultados indicam um comportamento incomum da GRASP em solução, similar à de uma classe de proteínas intrinsicamente desordenadas colapsadas conhecidas como glóbulos fundidos. Nós relatamos também as propensões de transição estrutural do tipo desordem-ordem para uma proteína glóbulo fundido nativa, induzidas pela presença de diferentes miméticos de condições celulares especificas. A mudança na constante dielétrica do meio (como as experimentadas próximas à superfície da membrana biológica) é o principal modulador estrutural, capaz de induzir múltiplas transições desordem-ordem na GRASP, sugerindo um comportamento muito distinto quando em condições que imitam a vizinhança da superfície da membrana em comparação com os encontrados quando livre em solução. Outros fatores de enovelamento, tais como o molecular crowding, contra-ions, pH e a fosforilação exibem efeitos menores (ou nenhum) na estrutura secundária e/ou estabilidade da GRASP. Este é o primeiro estudo focado na compreensão das transições desordem-ordem em uma estrutura do tipo glóbulo fundido sem que houvesse a necessidade de qualquer condição desnaturante. Em relação aos PDZs que formam o domínio GRASP, observamos que as GRASPs são formadas por um PDZ1 mais instável e flexível e um PDZ2 muito mais estável e estruturalmente bem comportado. Mais do que isso, muitas das regiões instáveis encontradas no PDZ1 estão no predito bolsão de ligação, sugerindo uma promiscuidade estrutural dentro desse domínio que se correlaciona com a promiscuidade funcional de interação com múltiplos parceiros proteicos. É apresentado nesta tese a primeira caracterização estrutural de uma GRASP em sua forma completa, o primeiro modelo de como as GRASPs (ou qualquer proteína em forma de glóbulo fundido) pode ser modulada estruturalmente pela célula durante diferentes funcionalidades e o primeiro trabalho na comunidade provando que a estabelecido ideia de que ambos os PDZs são estruturalmente equivalentes não é completamente correta

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Shekhawat, Sujan Singh. "Genetically Encoded Sensors for Detection of Proteases Utilizing Auto-Inhibited Coiled Coils and Split-Protein Reassembly." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/205209.

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The detection of cellular events is central to understanding biomoleculer processes as well as aid in therapeutic intervention strategies. One of the most fascinating biomoleculer events during the life cycle of a cell is proteolytic cleavage of proteins by enzymes known as proteases. Proteases are ubiquitous and participate in essential functions such as fertilization, embryo development, cell cycle regulation, immune response, tissue remodeling and programmed cell death. As proteases are involved in fundamental cellular processes any dysregulation of protease activity is usually associated with a diseased state. Thus methods for detection of protease activity are desirable as it may facilitate the identification of many pathological conditions which are associated with the aberrant expression and activity of proteases.Towards the goal of a general and modular strategy we have utilized split protein reassembly and coiled coils to develop genetically encoded sensors for detection of proteases. We established our first generation protease design utilizing split firefly luciferase and anti-parallel coiled coils and detected Tobacco Etch Virus (TEV) as a model protease. Two further iterations of the coiled-coil design led to the development of second and third generation of protease sensors which showed substantial improvement in the sensor response and was applied towards detection of therapeutically relevant proteases such as caspase-3, prostate specific antigen (PSA), ß-secretase and calpain-1.We applied our methodolgy to develop protease biosensors for the detection of a family of cysteine protease known as caspases. Caspases are involved in programmed cell death and their misregulation is implicated in cancer as well as neurodegenerative disorders. The panel of caspase biosensors was utilized to investigate caspase cleavage specificity as well as caspase activation in mammalian cytosolic extracts and live mammalian cells. Perhaps more importantly, we discovered cross talk between members of the caspase family which perform different biological functions.Finally, we detail our progress towards mimicking a naturally occurring multicomponent complex formed during programmed cell death, known as the apoptosome which leads to the activation of caspases. We have successfully utilized principles of self assembly and multivalency to assemble multi component complexes which exhibit proteolytic activity similar to the natural apoptosome.

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Porter, Jason Robert. "SPLIT-PROTEIN REASSEMBLY METHODS FOR THE DETECTION AND INTERROGATION OF BIOMOLECULAR INTERACTIONS AND MODULATORS THEREOF." Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/194359.

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The interactions between protein-protein, protein-nucleic acid, and protein-small molecules are central to biological processes and are key for the design of new therapeutics. Rapid and easy to implement methodologies are needed that enable the interrogation of these interactions in a complex cellular context. Towards this goal, I have utilized the concept of split-protein reassembly, also called protein complementation, for the creation of a variety of sensor architectures that enable the interrogation of protein-nucleic acid, protein-protein, and protein-small molecule interactions. Utilizing the enzymatic split-reporter β-lactamase and existing zinc finger design strategies we applied our recently developed technology termed SEquence-Enabled Reassembly (SEER) towards the creation of a sensor capable of the specific detection of the CryIA transgene. Additionally, the split β-lactamase reporter was utilized for the sitespecific determination of DNA methylation at cytosine residues that is involved in epigenetic regulation. This method, dubbed mCpG-SEER, enabled the direct detection of femtomole levels of dsDNA methylation in sequence specific manner. In a separate endeavor, we have developed and optimized the first cell-free split-reporter systems for GFP, split β-lactamase, and firefly luciferase for the successful dsDNA-dependent reassembly of the various reporters. Our cell free in vitro translation systems eliminates previous bottlenecks encountered in split-reporter technologies such as laborious transfection/cell culture or protein purification. Capitalizing on the ease of use and speed afforded by this new technology we describe the sensitive detection of protein-protein, protein-nucleic acid, and protein-small molecule interactions and inhibitors thereof. In a related area, we have applied this rapid cell-free split-firefly luciferase assay to the specific interrogation of a large class of helix-receptor protein-protein interactions. We have built a panel consisting of the clinically relevant Bcl-2 family of proteins, hDM2, hDM4, and p53 and interrogated the specificity of helix-receptor interactions as well as the specificity of peptide and small-molecule inhibitors of these interactions. Finally, we describe the further applications of our cell-free technology to the development of a large number of general split-firefly luciferase sensors for the detection of ssRNA sequences, the detection of native proteins, the evaluation of protease activity, and interrogation of enzyme-inhibitor interactions. The new methodologies provided in this study provides a general and enabling methodology for the rapid interrogation of a wide variety of biomolecular interactions and their antagonists without the limitations imposed by current in vitro and in vivo approaches.

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Furman, Jennifer Lynn. "IN VITRO AND IN VIVO DETECTION OF NUCLEIC ACIDS AND PROTEINS USING SPLIT-PROTEIN REASSEMBLY." Diss., The University of Arizona, 2010. http://hdl.handle.net/10150/195828.

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The ability to directly monitor the presence of specific proteins or nucleic acids in a variety of in vitro and in vivo contexts has great utility for understanding biology as well as for the development of diagnostic agents. Herein I describe several methodologies, utilizing split-protein reassembly, which provides potentially general strategies for sequence-specific detection of DNA and RNA sequences as well as poly(ADP-ribose). I also provide a new split-protein approach for the direct detection of native proteins, such as the cancer marker HER2.The green fluorescent protein (GFP) provides a convenient sensor for reporting on a variety of cellular events. A series of spectroscopically distinct GFP variants was developed for sequence-specifically reporting on DNA. Each of these variants was demonstrated to provide a sensitive readout for the presence of a particular DNA sequence. Furthermore, utilizing a method of mixed split-protein complementation, I was able to simultaneously report on the presence of two distinct DNA sequences in the same solution.To provide a general solution for reporting on the presence of particular RNA sequences, a method was developed that utilized elements from a hybridization-based detection strategy coupled with split-protein reassembly. Specifically, DNA guide sequences complementary to an intended target were attached to hairpin sites that served as binding sites for high-affinity zinc fingers. Localization of the zinc fingers allowed for reassembly of the attached split enzyme, providing a sensitive readout for the presence of potentially any RNA sequence of interest. This methodology was applied to the detection of mRNA encoding VEGF, hDM2, and HER2, each of which may be overexpressed in cancer.A method was established for reporting on the presence of modifications to DNA and proteins that are elicited in response to DNA damage. Specifically, sensors were designed, which incorporated endogenous damage-recognition domains, to report on the global presence of particular DNA modifications, including the formation of 8-oxoguanine and pyrimidine dimers. Furthermore, to provide a technique for monitoring the general accrual of DNA damage and to interrogate the DNA damage response in cells, a sensor was developed which reported on the accrual of a posttranslational protein modification, poly(ADP-ribosyl)ation.Finally, I describe advances toward the adaptation of our protein-based biosensors for use in living cells, utilizing both GFP-based approaches for live cell imaging as well as luminescent-based strategies for reporting on proteins and nucleic acids following cell lysis.

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Books on the topic "Reassembly"

1

B, Wood J. The Gun digest sporting rifle take down & reassembly guide. 2nd ed. Northfield, WI: DBI Books, 1997.

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Krakatau: The destruction and reassembly of an island ecosystem. Cambridge, Mass: Harvard University Press, 1996.

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Mowbray, Stuart C. A collector's guide to-- military pistol and revolver disassembly and reassembly. Woonsocket, Rhode Island: Mowbray Publishing, 2011.

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O'Caiside, Seamus. Beretta 391 disassembly: An illustrated manual for the disassembly and reassembly of Beretta 391 shotguns, with notes on cleaning, maintenance and modification. Richmond, VA: Published and printed by the author, 2007.

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Madsen, Catherine. The bones reassemble: Reconstituting liturgical speech. Aurora, Colo: Davies Group, 2005.

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1950-, Allen Robert Clyde, ed. Channels of discourse, reassembled: Television and contemporary criticism. 2nd ed. Chapel Hill: University of North Carolina Press, 1992.

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Flood, Katherine Devlin. Puzzle: Some Reassembly Required. Lulu Press, Inc., 2015.

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Walter, Scott. Mobilization and Reassembly of Genetic Information. Elsevier Science & Technology Books, 2012.

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Thornton, Ian. Krakatau: The Destruction and Reassembly of an Island Ecosystem. Harvard University Press, 1996.

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A Collector's Guide to Military Rifle Disassembly and Reassembly. Andrew Mowbray Publishers, Inc., 2007.

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Book chapters on the topic "Reassembly"

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Galatowitsch, Susan. "Plant Community Reassembly in Restored Wetlands." In The Wetland Book, 1–6. Dordrecht: Springer Netherlands, 2016. http://dx.doi.org/10.1007/978-94-007-6172-8_327-1.

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Galatowitsch, Susan. "Plant Community Reassembly in Restored Wetlands." In The Wetland Book, 2003–8. Dordrecht: Springer Netherlands, 2018. http://dx.doi.org/10.1007/978-90-481-9659-3_327.

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Zhang, Meng, and Jiu-bin Ju. "Space-Economical Reassembly for Intrusion Detection System." In Information and Communications Security, 393–404. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-540-39927-8_36.

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Hercog, Drago. "Segmentation and Reassembly of Protocol Data Units." In Communication Protocols, 109–15. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-50405-2_9.

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Yin, Congli, Mingquan Zhou, Yachun Fan, and Wuyang Shui. "Template-Guided 3D Fragment Reassembly Using GDS." In Image and Graphics Technologies and Applications, 432–41. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1702-6_43.

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Wu, Mengmeng, and Jianfeng (Jeff) Wang. "An Investigation of 3D Sand Particle Fragment Reassembly." In Trends in Mathematics, 383–90. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-99474-1_38.

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Tzur, Yonatan B., and Yosef Gruenbaum. "Nuclear Envelope Breakdown and Reassembly in C. elegans." In Nuclear Envelope Dynamics in Embryos and Somatic Cells, 103–10. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0129-9_8.

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Heijenk, Geert J., and Ignas G. Niemegeers. "Modelling the Reassembly Buffer in a Connectionless Server." In Performance Modelling and Evaluation of ATM Networks, 573–95. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-0-387-34881-0_31.

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Schwingshackl, C. W. "Identification Reassembly Uncertainties for a Basic Lap Joint." In Dynamics of Coupled Structures, Volume 4, 53–61. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-54930-9_6.

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Jana, Susovan. "A Comparative Study on Reassembly of Image Fragments." In Algorithms for Intelligent Systems, 235–42. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-1657-1_19.

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Conference papers on the topic "Reassembly"

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Abrons, Ellie, Meredith Miller, Adam Fure, and Thom Moran. "Reassembly." In 2018 ACSA International Conference. ACSA Press, 2018. http://dx.doi.org/10.35483/acsa.intl.2018.10.

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The story of post-industrial urban decline in America is well known. Bustling cities fall victim to changing economic structures and globalization. Wealth moves out of city centers, leaving behind evacuated buildings and vacant lots where houses once stood and transforming vibrant neighborhoods into sparsely populated areas that lack the density necessary to sustain urban life. Municipalities deem abandoned buildings “blight” and assemble task forces to eradicate them. In response to this pressing urban reality, we have been developing a speculative approach to reusing buildings and materials called “reassembly.” Reassembly views a building’s materiality as a matter-of-fact, as a resource for architecture stripped of the negative assumptions commonly associated with disused properties. Building components are taken apart, moved around, piled up, and mixed with new construction to create alternative uses and forms.

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Chandranmenon, Girish P., and George Varghese. "Reconsidering fragmentation and reassembly." In the seventeenth annual ACM symposium. New York, New York, USA: ACM Press, 1998. http://dx.doi.org/10.1145/277697.277703.

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Wang, Ruoyu, Yan Shoshitaishvili, Antonio Bianchi, Aravind Machiry, John Grosen, Paul Grosen, Christopher Kruegel, and Giovanni Vigna. "Ramblr: Making Reassembly Great Again." In Network and Distributed System Security Symposium. Reston, VA: Internet Society, 2017. http://dx.doi.org/10.14722/ndss.2017.23225.

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Pal, A., K. Shanmugasundaram, and N. Memon. "Automated reassembly of fragmented images." In 2003 International Conference on Multimedia and Expo. ICME '03. Proceedings (Cat. No.03TH8698). IEEE, 2003. http://dx.doi.org/10.1109/icme.2003.1220995.

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Vu, Tran Huy, Nguyen Quoc Tuan, Tran Ngoc Thinh, and Nguyen Tran Huu Nguyen. "Memory-efficient TCP reassembly using FPGA." In the Second Symposium. New York, New York, USA: ACM Press, 2011. http://dx.doi.org/10.1145/2069216.2069261.

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Wang, Jiaqi, Kai Chen, Rui Xu, Ziwei Liu, Chen Change Loy, and Dahua Lin. "CARAFE: Content-Aware ReAssembly of FEatures." In 2019 IEEE/CVF International Conference on Computer Vision (ICCV). IEEE, 2019. http://dx.doi.org/10.1109/iccv.2019.00310.

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Chang, Hyunseok, Fang Hao, T. V. Lakshman, Sarit Mukherjee, and Limin Wang. "Cache-Friendly IP Reassembly Network Function." In SOSR '20: Symposium on SDN Research. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3373360.3380837.

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Yim, Mark, Babak Shirmohammadi, Jimmy Sastra, Michael Park, Michael Dugan, and C. J. Taylor. "Towards robotic self-reassembly after explosion." In 2007 IEEE/RSJ International Conference on Intelligent Robots and Systems. IEEE, 2007. http://dx.doi.org/10.1109/iros.2007.4399594.

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Du, Guoguang, Mingquan Zhou, Congli Yin, Zhongke Wu, and Wuyang Shui. "Classification and reassembly of archaeological fragments." In the Symposium. New York, New York, USA: ACM Press, 2016. http://dx.doi.org/10.1145/3014027.3014031.

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Andreadis, Anthousis, Georgios Papaioannou, and Pavlos Mavridis. "Generalized digital reassembly using geometric registration." In 2015 Digital Heritage. IEEE, 2015. http://dx.doi.org/10.1109/digitalheritage.2015.7419572.

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Reports on the topic "Reassembly"

1

Heffner, J., M. Mathis, and B. Chandler. IPv4 Reassembly Errors at High Data Rates. RFC Editor, July 2007. http://dx.doi.org/10.17487/rfc4963.

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Malis, A., and M. Townsley. Pseudowire Emulation Edge-to-Edge (PWE3) Fragmentation and Reassembly. RFC Editor, August 2006. http://dx.doi.org/10.17487/rfc4623.

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Haq, Mahmoodul, and Lawrence T. Drzal. Active, tailorable adhesives for dissimilar material bonding, repair and reassembly. Office of Scientific and Technical Information (OSTI), March 2019. http://dx.doi.org/10.2172/1497730.

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Ohad, Itzhak, and Himadri Pakrasi. Role of Cytochrome B559 in Photoinhibition. United States Department of Agriculture, December 1995. http://dx.doi.org/10.32747/1995.7613031.bard.

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The aim of this research project was to obtain information on the role of the cytochrome b559 in the function of Photosystem-II (PSII) with special emphasis on the light induced photo inactivation of PSII and turnover of the photochemical reaction center II protein subunit RCII-D1. The major goals of this project were: 1) Isolation and sequencing of the Chlamydomonas chloroplast psbE and psbF genes encoding the cytochrome b559 a and b subunits respectively; 2) Generation of site directed mutants and testing the effect of such mutation on the function of PSII under various light conditions; 3) To obtain further information on the mechanism of the light induced degradation and replacement of the PSII core proteins. This information shall serve as a basis for the understanding of the role of the cytochrome b559 in the process of photoinhibition and recovery of photosynthetic activity as well as during low light induced turnover of the D1 protein. Unlike in other organisms in which the psbE and psbF genes encoding the a and b subunits of cytochrome b559, are part of an operon which also includes the psbL and psbJ genes, in Chlamydomonas these genes are transcribed from different regions of the chloroplast chromosome. The charge distribution of the derived amino-acid sequences of psbE and psbF gene products differs from that of the corresponding genes in other organisms as far as the rule of "positive charge in" is concerned relative to the process of the polypeptide insertion in the thylakoid membrane. However, the sum of the charges of both subunits corresponds to the above rule possibly indicating co-insertion of both subunits in the process of cytochrome b559 assembly. A plasmid designed for the introduction of site-specific mutations into the psbF gene of C. reinhardtii. was constructed. The vector consists of a DNA fragment from the chromosome of C. reinhardtii which spans the region of the psbF gene, upstream of which the spectinomycin-resistance-conferring aadA cassette was inserted. This vector was successfully used to transform wild type C. reinhardtii cells. The spectinomycin resistant strain thus obtained can grow autotrophically and does not show significant changes as compared to the wild-type strain in PSII activity. The following mutations have been introduced in the psbF gene: H23M; H23Y; W19L and W19. The replacement of H23 involved in the heme binding to M and Y was meant to permit heme binding but eventually alter some or all of the electron transport properties of the mutated cytochrome. Tryptophane W19, a strictly conserved residue, is proximal to the heme and may interact with the tetrapyrole ring. Therefore its replacement may effect the heme properties. A change to tyrosine may have a lesser affect on the potential or electron transfer rate while a replacement of W19 by leucine is meant to introduce a more prominent disturbance in these parameters. Two of the mutants, FW19L and FH23M have segregated already and are homoplasmic. The rest are still grown under selection conditions until complete segregation will be obtained. All mutants contain assembled and functional PSII exhibiting an increased sensitivity of PSII to the light. Work is still in progress for the detailed characterization of the mutants PSII properties. A tobacco mutant, S6, obtained by Maliga and coworkers harboring the F26S mutation in the b subunit was made available to us and was characterized. Measurements of PSII charge separation and recombination, polypeptide content and electron flow indicates that this mutation indeed results in light sensitivity. Presently further work is in progress in the detailed characterization of the properties of all the above mutants. Information was obtained demonstrating that photoinactivation of PSII in vivo initiates a series of progressive changes in the properties of RCII which result in an irreversible modification of the RCII-D1 protein leading to its degradation and replacement. The cleavage process of the modified RCII-D1 protein is regulated by the occupancy of the QB site of RCII by plastoquinone. Newly synthesized D1 protein is not accumulated in a stable form unless integrated in reassembled RCII. Thus the degradation of the irreversibly modified RCII-D1 protein is essential for the recovery process. The light induced degradation of the RCII-D1 protein is rapid in mutants lacking the pD1 processing protease such as in the LF-1 mutant of the unicellular alga Scenedesmus obliquus. In this case the Mn binding site of PSII is abolished, the water oxidation process is inhibited and harmful cation radicals are formed following light induced electron flow in PSII. In such mutants photo-inactivation of PSII is rapid, it is not protected by ligands binding at the QB site and the degradation of the inactivated RCII-D1 occurs rapidly also in the dark. Furthermore the degraded D1 protein can be replaced in the dark in absence of light driven redox controlled reactions. The replacement of the RCII-D1 protein involves the de novo synthesis of the precursor protein, pD1, and its processing at the C-terminus end by an unknown processing protease. In the frame of this work, a gene previously isolated and sequenced by Dr. Pakrasi's group has been identified as encoding the RCII-pD1 C-terminus processing protease in the cyanobacterium Synechocystis sp. PCC 6803. The deduced sequence of the ctpA protein shows significant similarity to the bovine, human and insect interphotoreceptor retinoid-binding proteins. Results obtained using C. reinhardtii cells exposes to low light or series of single turnover light flashes have been also obtained indicating that the process of RCII-D1 protein turnover under non-photoinactivating conditions (low light) may be related to charge recombination in RCII due to back electron flow from the semiquinone QB- to the oxidised S2,3 states of the Mn cluster involved in the water oxidation process.

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