Relevant bibliographies by topics / Realtime qPCR

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  1. Journal articles
  2. Dissertations / Theses

Academic literature on the topic 'Realtime qPCR'

Author: Grafiati
Published: 9 March 2023
Last updated: 31 July 2025

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Journal articles on the topic "Realtime qPCR"

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Marnis, Huria, Bambang Iswanto, Romy Suprapto, Imron Imron, and Raden Roro Sri Pudji Sinarni Dewi. "IDENTIFIKASI ZIGOSITAS IKAN LELE (Clarias gariepinus) TRANSGENIK F-2 YANG MEMBAWA GEN HORMON (PhGH) DENGAN MENGGUNAKAN METODE REALTIME-qPCR." Jurnal Riset Akuakultur 11, no. 1 (2016): 39. http://dx.doi.org/10.15578/jra.11.1.2016.39-46.

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Produktivitas ikan budidaya dapat ditingkatkan melalui teknologi transgenesis. Populasi ikan lele transgenik cepat tumbuh telah dihasilkan dan karakter biologisnya telah diketahui. Namun informasi zigositas ikan lele transgenik perlu ditelaah lebih lanjut. Penelitian ini bertujuan untuk mengidentifikasi zigositas ikan lele transgenik F-2. Zigositas ikan lele transgenik diidentifikasi dengan menggunakan metode real-time qPCR (RT-qPCR) dan uji progeni. Identifikasi zigositas melalui uji progeni, dilakukan dengan mendeteksi transgen (PhGH) pada individu-individu F-3 hasil persilangan transgenik F
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Hikmawati, Isna, Hendro Wahjono, Martini Martini, et al. "Persistence of Dengue Virus (DENV-1, 2, 3,4) Transovarial-Transgenerational with Realtime Polymerase Chain Reaction (qPCR) in Ae. Aegypti and Ae. Albopictus (Diptera: Culicidae)." Women Health Care and Issues 4, no. 8 (2021): 01–09. http://dx.doi.org/10.31579/2642-9756/090.

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Ae. aegypti and Ae. albopictus have an important role in DHF transmission because they can simultaneously transmit the dengue virus vertically / transovarially or horizontally. This phenomenon indicates the persistence of the dengue virus by vectors. The aim of this research was to prove the persistence of the transovarial-transgenerational dengue virus (DENV-1,2,3,4) with real time polymerase chain reaction (qPCR) in Ae. aegypti and Ae. albopictus (Diptera: Culicidae). Quasi experimental design with intervention infects DENV 1-2-3-4 serotypes in Ae. aegypti and Ae. albopictus intratoracally.
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Amor, Isabel, Ana Alberola, Adolfo De Salazar, et al. "Evaluation of the Vaginal Panel Realtime PCR kit (Vircell, SL) for diagnosing vaginitis: A comparative study with routinely used diagnostics." PLOS ONE 19, no. 11 (2024): e0313414. http://dx.doi.org/10.1371/journal.pone.0313414.

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Vaginitis is a prevalent clinical disorder associated with several adverse health consequences, prompting women to seek medical care. In this study we evaluate the Vaginal Panel Real-Time PCR kit (qPCR test) against routinely used diagnostics for detection of bacterial vaginosis (BV), vulvovaginal candidiasis (VVC), and trichomoniasis. A total of 1011 vaginal swab specimens were analyzed. The routinely diagnostic methods for BV was Gram stain-based Nugent score. VVC presence was detected by culture, and Candida species were identified using MALDI-TOF MS. Trichomonas vaginalis was identified by
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Okangba, C. C., T. A. Ajani, G. C. Okangba, et al. "Evaluation of the detection of Plasmodium falciparum infection in Urine, Plasma, Serum, and Dried Blood Spots using real-time Polymerase Chain React." Nigerian Journal of Parasitology 44, no. 1 (2023): 78–86. http://dx.doi.org/10.4314/njpar.v44i1.7.

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Access to timely and accurate diagnostic tests has a significant impact on the management of malaria disease which is a global concern. Polymerase chain reaction (PCR) detection of Plasmodium DNA is highly sensitive in diagnosing malaria. The specimen of choice for this assay has been whole blood samples from patients with malaria caused by Plasmodium species. Nucleic acids can also be detected in urine, serum, plasma, and Dried Blood Spots (DBS) samples but there are few studies describing the diagnostic performance of PCR. Therefore, this study was aimed at evaluating the performance of real
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Rublenko, N. "Identification of Salmonella spp and serovars Typhimurium, Enteritidis by qPCR." Naukovij vìsnik veterinarnoï medicini, no. 1(154) (May 21, 2020): 21–31. http://dx.doi.org/10.33245/2310-4902-2020-154-1-21-31.

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This article presents the results of the identification of the Salmonella genus as well as serovars Enteritidis and Typhimurium by a real-time polymerase chain reaction. We constructed three pairs of primers and fluorescent probes to simultaneously identify the Salmonella genus, serovars Enteritidis and Typhimurium in a qPCR. The specificity of the primers was evaluated on Salmonella strains of different serovars from the National Center for Strains of Microorganisms (UNCMS) strains of the State Scientific Control Institute of Biotechnology and Strains of Microorganisms (SSCIBSM) and 46 Salmon
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Dyer, Clare E. F., Naomi E. Clarke, Dinh Ng Nguyen, et al. "Assessing the efficacy of albendazole against hookworm in Vietnam using quantitative PCR and sodium nitrate flotation." PLOS Neglected Tropical Diseases 16, no. 10 (2022): e0010767. http://dx.doi.org/10.1371/journal.pntd.0010767.

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Preventive chemotherapy (PC), consisting of the regular distribution of anthelmintics to populations or groups of populations at risk, is the primary tool used to control soil-transmitted helminth (STH) infections. This strategy, whilst cost-effective, raises the concern of potential emergence of drug resistance. The efficacy of anthelmintics against STH infections is measured using cure rate (CR) and egg reduction rate (ERR), using microscopy-based techniques such as the Kato-Katz thick smear. However, Kato-Katz has low sensitivity, especially for low-intensity infections, and requires fresh
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Rasmussen, S., A. J. Parsons, Q. Liu, H. Xue, and J. A. Newman. "High nutrient supply and carbohydrate content reduce endophyte and alkaloid concentration." NZGA: Research and Practice Series 13 (January 1, 2007): 135–38. http://dx.doi.org/10.33584/rps.13.2006.3103.

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Two controlled environment experiments were performed to test the effects of nitrogen, phosphorus and carbohydrates on endophyte (Neotyphodium lolii) and alkaloid concentrations in ryegrass (Lolium perenne). Three perennial ryegrass cultivars ('high sugar grasses' AberDove and AberDart; control Fennema) that differ in carbohydrate content were infected with three strains of N. lolii (common strain, CS; AR1; AR37). Infected and uninfected plants were grown under high (9 mM) and low (2.25 mM) nitrogen (AberDove, Fennema; CS, AR1, AR37) or under high (2 mM KH2PO4) and low (0.05 mM KH2PO4) phospho
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Karolak, Justyna A., Barbara Ginter-Matuszewska, Katarzyna Tomela, et al. "Further evaluation of differential expression of keratoconus candidate genes in human corneas." PeerJ 8 (August 20, 2020): e9793. http://dx.doi.org/10.7717/peerj.9793.

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Background Keratoconus (KTCN) is a progressive eye disease, characterized by changes in the shape and thickness of the cornea that results in loss of visual acuity. While numerous KTCN candidate genes have been identified, the genetic etiology of the disease remains undetermined. To further investigate and verify the contribution of particular genetic factors to KTCN, we assessed 45 candidate genes previously indicated as involved in KTCN etiology based on transcriptomic and genomic data. Methods The RealTime ready Custom Panel, covering 45 KTCN candidate genes and two reference transcripts, h
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Długosz-Pokorska, Angelika, Tomasz Janecki, Anna Janecka, and Katarzyna Gach-Janczak. "Synergistic Effects of Oxaliplatin, 5-Fluorouracil, and Novel Synthetic Uracil Analog U-359 on Breast Cancer Cell Carcinogenesis." International Journal of Molecular Sciences 26, no. 7 (2025): 2964. https://doi.org/10.3390/ijms26072964.

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Breast cancer presents significant global challenges, necessitating effective treatments to combat drug resistance and minimize chemotherapy side effects. This study evaluated the cytotoxic effects of U-359, Oxaliplatin (Ox), and 5-Fluorouracil (5-FU) in MCF-7 and MCF-10A cells using MTT and RealTime-GLO assays. Morphological changes were assessed by light microscopy following Wright–Giemsa staining. Apoptosis induction was studied using qPCR for apoptotic markers, the RealTime-Glo™ Annexin V assay, and the cleaved PARP1 ELISA assay. Caspase 8 and 9 activities, ABCB1, ABCG2, and NF-κB protein
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Weng, Wenfeng, Xiang Lu, Meiliang Zhou, et al. "FtbZIP12 Positively Regulates Responses to Osmotic Stress in Tartary Buckwheat." International Journal of Molecular Sciences 23, no. 21 (2022): 13072. http://dx.doi.org/10.3390/ijms232113072.

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ABFs play a key role in regulating plant osmotic stress. However, in Tartary buckwheat, data on the role of ABF genes in osmotic stress remain limited and its associated mechanism in osmoregulation remain nebulous. Herein, a novel ABF family in Tartary buckwheat, FtbZIP12, was cloned and characterized. FtbZIP12 is a transcriptional activator located in the nucleus; its expression is induced by NaCl, mannitol, and abscisic acid (ABA). Atopic expression of FtbZIP12 in Arabidopsis promoted seed germination, reduced damage to primary roots, and improved the tolerance of seedlings to osmotic stress
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Dissertations / Theses on the topic "Realtime qPCR"

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POZZI, STEFANO. "Caratterizzazione del trascrittoma di PBMCs di pazienti affetti da aneurisma dell'aorta addominale e da ostruzione carotidea." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2009. http://hdl.handle.net/10281/7479.

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Atherosclerosis (also known as Arteriosclerotic Vascular Disease or ASVD) is the condition in which an artery wall thickens as the result of a build-up of fatty materials such as cholesterol. It is a syndrome affecting arterial blood vessels, a chronic inflammatory response in the walls of arteries, in large part due to the accumulation of macrophage white blood cells and promoted by Low-density lipoproteins (plasma proteins that carry cholesterol and triglycerides) without adequate removal of fats and cholesterol from the macrophages by functional high density lipoproteins (HDL). It is common
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