Academic literature on the topic 'Real-time PCR'
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Journal articles on the topic "Real-time PCR"
RAZA, ABIDA, and NAUREEN A KHATTAK. "REAL TIME PCR;." Professional Medical Journal 19, no. 06 (November 3, 2012): 751–59. http://dx.doi.org/10.29309/tpmj/2012.19.06.2455.
Full textLederman, Lynne. "Real-Time PCR." BioTechniques 44, no. 2 (February 2008): 179–83. http://dx.doi.org/10.2144/000112741.
Full textDavidson, Eugene A. "REAL-TIME PCR." Shock 27, no. 6 (June 2007): 708. http://dx.doi.org/10.1097/01.shk.0000270193.65250.8e.
Full textKusser, Wolfgang, Sandrine Javorschi, and Martin A. Gleeson. "Real-Time PCR." Cold Spring Harbor Protocols 2006, no. 1 (January 1, 2006): pdb.prot4112. http://dx.doi.org/10.1101/pdb.prot4112.
Full textFraga, Dean, Tea Meulia, and Steven Fenster. "Real-Time PCR." Current Protocols Essential Laboratory Techniques 00, no. 1 (January 2008): 10.3.1–10.3.34. http://dx.doi.org/10.1002/9780470089941.et1003s00.
Full textBusch, Ulrich. "Real-Time PCR." Journal für Verbraucherschutz und Lebensmittelsicherheit 2, no. 2 (May 2007): 111–12. http://dx.doi.org/10.1007/s00003-007-0178-7.
Full textKang, Won, Sang-Bum Park, Youn-Hyoung Nam, Young-Chang An, Sang-Hyun Lee, Won-Cheoul Jang, Su-Min Park, Jong-Wan Kim, and Song-Chun Chong. "Detection of Hepatitis B Virus Using Micro-PCR and Real-Time PCR Methods." Journal of the Korean Chemical Society 51, no. 1 (February 20, 2007): 36–42. http://dx.doi.org/10.5012/jkcs.2007.51.1.036.
Full textHeid, C. A., J. Stevens, K. J. Livak, and P. M. Williams. "Real time quantitative PCR." Genome Research 6, no. 10 (October 1, 1996): 986–94. http://dx.doi.org/10.1101/gr.6.10.986.
Full textSchmittgen, Thomas D. "Real-Time Quantitative PCR." Methods 25, no. 4 (December 2001): 383–85. http://dx.doi.org/10.1006/meth.2001.1260.
Full textGospodinović, Hristina, Ljiljana Pavlović, Marija Obradović, Sanja Dimitrijević, Sofija Jovanović, and Edita Grego. "Detection of high-risk HPV genotypes using Real-time PCR." Glasnik javnog zdravlja 96, no. 4 (2022): 416–26. http://dx.doi.org/10.5937/serbjph2204416g.
Full textDissertations / Theses on the topic "Real-time PCR"
Crane, Bryan Lee 1976. "Real time PCR measurement by fluorescence anisotropy." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/30347.
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Includes bibliographical references (p. 181-190).
Real-time polymerase chain reaction (PCR) is the gold-standard for quantitation in both mutation and gene expression analyses. Already this technique has found valuable clinical application in disease diagnosis and progression evaluation. As the number of known gene-disease correlations continues to rise, there will be increased demand for higher throughput and decreased cost for these analyses. Present real-time PCR measurement is based upon the fluorescent intensity of either intercalating dyes or oligonucleotide probes. Intercalating dye methods suffer from a lack of binding specificity, while probe methods are expensive and require increased assay optimization. In this thesis, a new method is presented for monitoring real-time PCR that utilizes the fluorescent anisotropy (FA) of labeled primers. FA, when measured at constant temperature, is indicative of the molecular mass to which the fluorophore is attached. Specificity is improved with the FA method over the use of intercalating dyes since the selective binding of primers is required for signal change. Assay complexity and cost are reduced compared to fluorogenic probe methods since the probes are eliminated. The design of a prototype instrument, which successfully implements this new method, is presented. Instrument and assay performance are compared to intercalating dye assays run in commercially available instrumentation. Theoretical limits on performance are also presented and compared to experimental results. Excellent repeatability and linearity are observed with respect to these benchmarks. This new method, having both high specificity and low optimization complexity, is expected to be particularly applicable to the demanding robustness requirements of nano-scale PCR.
by Bryan Lee Crane.
Ph.D.
Rozales, Franciéli Pedrotti. "Real time-pcr e nested-pcr no diagnóstico da tuberculose pulmonar." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/72990.
Full textTuberculosis (TB) remains as an important public health problem worldwide. Therefore, the rapid detection of M. tuberculosis is of primary importance to effectively reduce transmission among patients. The aims of this study were to evaluate two molecular tests to detect M. tuberculosis complex (MTBC) directly from clinical samples. The study included 124 respiratory samples which were evaluated by two in house molecular assays for MTBC detection: Nested PCR (NPCR) and Real Time PCR (RT-PCR). The respiratory samples were also evaluated by the direct test (AFB assay). The results were compared with the results of culture and also compared with the culture results plus clinical data of patients. We used a commercial DNA sample with known quantification to establish the Limit of Detection (LOD). The LOD was 1 copy/μL for RT-PCR and 25 copies/μL for NPCR. The AFB assay presented low sensitivity – SE - (40%) and a high specificity - SP – (94%). Both molecular assays, RT-PCR and NPCR presented high SE and SP (RT-PCR 98% and 91%, NPCR 86% and 93%, respectively) compared to culture. When the results of the molecular tests were compared to the culture plus clinical data the SE and SP were 90,20% and 97,26% for RT-PCR and 80,39% and 98,63% for the NPCR, respectively. It was possible to observe a slight decrease of SE of the molecular methods in comparison to culture plus clinical data in relation to culture; however, the SP was increased, since many cases of TB could not be confirmed by culture. Furthermore we evaluated the cost of molecular assays: the NPCR cost was $17.77/test while the RT-PCR cost was $15.76/test. The RT-PCR test was faster (2 hours) than the NPCR (4 hours) to be performed. Our study confirms that PCRs may be useful for rapid diagnosis of respiratory TB, with high SP rates. It may also be very important to exclude such diagnosis, considering the high NPV found in our study. In summary, PCRs targeting IS6110 of MTB improve the accuracy of the diagnosis of pulmonary TB, with many potential positive effects for clinical management and control of the disease.
Pires, Elisabete Sofia Videira. "Real-Time PCR, High Resolution Melting - aplicações forenses." Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/10747.
Full textApontada como a maior revolução científica na área forense desde a descoberta das impressões digitais, a identificação humana por meio da análise do DNA tornou-se uma poderosa ferramenta de investigação, auxiliando na elucidação de casos forenses, baseando-se cientificamente na existência de polimorfismos genéticos ao longo do genoma em indivíduos diferentes, que faz com que cada pessoa possua um código genético único. Com a introdução da real-time PCR nas investigações forenses, tornou-se possível uma análise sensível e específica de regiões polimórficas tanto no genoma nuclear como no mitocondrial, a partir de quantidades ínfimas de DNA obtidas de amostras altamente degradadas ou com baixo número de cópias. A quantificação do DNA é um procedimento importante na análise forense e deve ser efetuado, previamente, a qualquer análise de DNA. A união entre a bioinformática e a genética forense propiciou a criação de métodos de análise específicos, como a HRM, muito útil na genotipagem de SNPs, de extrema importância na investigação forense. Foi elaborada uma revisão bibliográfica com o objetivo de conhecer as aplicações forenses da real-time PCR e os respetivos métodos, tendo se confirmado então a aplicabilidade deste método na área forense.
Listed as the greatest revolution in forensic science since the discovery of fingerprints, identification by analyzing human DNA has become a powerful research tool, helping to elucidate forensic cases, scientifically based on the existence of genetic polymorphisms throughout the genome at different individuals, which causes that each person has a unique genetic code. With the introduction of real-time PCR in forensic investigations, it became possible a sensitive and specific analysis of polymorphic regions both in the mitochondrial and nuclear genome, from minute quantities of DNA obtained from samples highly degraded or low copy number. The quantification of DNA is an important procedure in forensic analysis and must be made in advance to any DNA analysis. The union between forensic genetics and bioinformatics led to the creation of specific analysis methods, such as HRM, very useful in scanning and genotyping of SNPs, of utmost importance in forensic investigation. A literature review has been prepared in order to meet the forensic applications of real-time PCR and related methods, and so been confirmed the applicability of this method in the forensic field.
Dunkley, Kingsley Delroy. "Modulation of cell yields and genetic responses of Salmonella fermentation and colonization in the gastrointestinal ecology of avian species." [College Station, Tex. : Texas A&M University, 2006. http://hdl.handle.net/1969.1/ETD-TAMU-1187.
Full textAndalo, Alice. "Analisi quantitativa dell'espressione genica mediante real-time rt-pcr." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/8450/.
Full textDörries, Hans-Henno. "Entwicklung von Real-Time-PCR-Nachweissystemen für getränkerelevante Hefen." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=979663938.
Full textHartmann, Britta. "Entwicklung einer Real-time-PCR-Nachweismethode für Yersinia enterocolitica." [S.l.] : [s.n.], 2007. http://edoc.ub.uni-muenchen.de/archive/00006660.
Full textHartmann, Britta. "Entwicklung einer Real-Time PCR-Nachweismethode für Yersinia enterocolitica." Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-66603.
Full textMalatji, Dikeledi Petunia. "Detection of Babesia rossi genotypes using real-time PCR." Diss., University of Pretoria, 2011. http://hdl.handle.net/2263/31138.
Full textDissertation (MSc)--University of Pretoria, 2011.
Veterinary Tropical Diseases
MSc
Unrestricted
Zhang, Yan. "Frequent RASSF1A gene promoter hypermethylation in breast cancer." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-63611.
Full textBooks on the topic "Real-time PCR"
Tevfik, Dorak M., ed. Real-time PCR. New York: Taylor & Francis, 2006.
Find full textTevfik, Dorak M., ed. Real-time PCR. New York: Taylor & Francis, 2006.
Find full textBiassoni, Roberto, and Alessandro Raso, eds. Quantitative Real-Time PCR. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0733-5.
Full textBiassoni, Roberto, and Alessandro Raso, eds. Quantitative Real-Time PCR. New York, NY: Springer New York, 2020. http://dx.doi.org/10.1007/978-1-4939-9833-3.
Full textMeuer, Stefan, Carl Wittwer, and Kan-Ichi Nakagawara, eds. Rapid Cycle Real-Time PCR. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0.
Full textQuantitative real-time PCR in applied microbiology. Norfolk, UK: Caister Academic Press, 2012.
Find full textJulie, Logan, Edwards Kirstin, and Saunders Nick, eds. Real-time PCR: Current technology and applications. Norfolk, UK: Caister Academic Press, 2009.
Find full textQuantitative real-time PCR: Methods and protocols. New York: Humana Press, 2014.
Find full textDietmaier, Wolfgang, Carl Wittwer, and Natarajan Sivasubramanian, eds. Rapid Cycle Real-Time PCR — Methods and Applications. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-59397-0.
Full textWittwer, Carl, Meinhard Hahn, and Karen Kaul, eds. Rapid Cycle Real-Time PCR — Methods and Applications. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18840-4.
Full textBook chapters on the topic "Real-time PCR"
Müller, Hans-Joachim, and Daniel Ruben Prange. "Real-Time-PCR." In PCR - Polymerase-Kettenreaktion, 65–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-48236-0_14.
Full textMathew, Alan G. "Real-Time PCR." In Handbook of Food Safety Engineering, 217–57. Oxford, UK: Wiley-Blackwell, 2012. http://dx.doi.org/10.1002/9781444355321.ch10.
Full textSaunders, Nicholas A. "Real-Time PCR." In Genomics, Proteomics, and Clinical Bacteriology, 191–211. Totowa, NJ: Humana Press, 2004. http://dx.doi.org/10.1385/1-59259-763-7:191.
Full textEvrard, A., N. Boulle, and G. s. Lutfalla. "Real-Time PCR." In Nanoscience, 841–69. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-88633-4_15.
Full textKonrad, Regina, and Ulrich Busch. "PCR und Real-Time PCR." In Molekularbiologische Methoden in der Lebensmittelanalytik, 35–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10716-0_4.
Full textSluijter, J. P. G., G. Pasterkamp, and D. P. V. de Kleijn. "Quantitative Real-Time PCR." In Cardiovascular Research, 75–83. Boston, MA: Springer US, 2006. http://dx.doi.org/10.1007/0-387-23329-6_4.
Full textBroll, Hermann. "Quantitative Real-Time PCR." In Molecular Biological and Immunological Techniques and Applications for Food Chemists, 59–83. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470637685.ch3.
Full textBartholomew, Rachel A., Janine R. Hutchison, Timothy M. Straub, and Douglas R. Call. "PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification." In Manual of Environmental Microbiology, 2.3.2–1–2.3.2–13. Washington, DC, USA: ASM Press, 2015. http://dx.doi.org/10.1128/9781555818821.ch2.3.2.
Full textSchmittgen, Thomas D., Eun Joo Lee, and Jinmai Jiang. "High-Throughput Real-Time PCR." In Methods in Molecular Biology, 89–98. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-040-3_7.
Full textDotti, Isabella, Ermanno Nardon, Danae Pracella, and Serena Bonin. "Quantitative Real-Time RT-PCR." In Guidelines for Molecular Analysis in Archive Tissues, 121–32. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_25.
Full textConference papers on the topic "Real-time PCR"
Becker, Holger, Nadine Hlawatsch, Richard Klemm, Christian Moche, Thomas Hansen-Hagge, and Claudia Gärtner. "Real-time PCR in microfluidic devices." In SPIE MOEMS-MEMS, edited by Bonnie L. Gray and Holger Becker. SPIE, 2014. http://dx.doi.org/10.1117/12.2037241.
Full textWoudenberg, Timothy M., and J. Stevens. "Quantitative PCR by real-time detection." In Photonics West '96, edited by Gerald E. Cohn, Steven A. Soper, and C. H. Winston Chen. SPIE, 1996. http://dx.doi.org/10.1117/12.237619.
Full textBARLOCCHI, G., U. MASTROMATTEO, S. SASSOLINI, M. SCURATI, and F. VILLA. "MICROFLUIDIC DEVICE FOR REAL TIME PCR DETECTION." In Proceedings of the 9th Italian Conference. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812701770_0058.
Full textGANDELMAN, O. A., V. L. CHURCH, C. A. MOORE, C. CARNE, H. JALAL, J. A. H. MURRAY, and L. C. TISI. "BART – BIOLUMINESCENT ALTERNATIVE TO REAL-TIME PCR." In Chemistry, Biology and Applications. WORLD SCIENTIFIC, 2007. http://dx.doi.org/10.1142/9789812770196_0023.
Full textChen, Ping-Hei, Da-Sheng Lee, Jui-Hung Chien, and Meng-Hsun Wu. "Development of a Novel Real-Time PCR Machine." In European Conference on Biomedical Optics. Washington, D.C.: OSA, 2005. http://dx.doi.org/10.1364/ecbo.2005.wg7.
Full textKubista, Mikael, Anders Stalberg, and Tzachi Bar. "Light-up-probe-based real-time Q-PCR." In BiOS 2001 The International Symposium on Biomedical Optics, edited by Ramesh Raghavachari and Weihong Tan. SPIE, 2001. http://dx.doi.org/10.1117/12.424589.
Full textHertwig, Aline Morgan von, Maristela da Silva do Nascimento, Maria Helena Pelegrinelli Fungaro, and Marta Hiromi Taniwaki. "Real-Time Pcr for Identification of Aspergillus Niger." In XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-077.
Full textLee, D. S., J. H. Chien, M. H. Wu, and P. H. Chen. "Development of a novel real-time PCR machine." In European Conference on Biomedical Optics 2005, edited by Christian D. Depeursinge. SPIE, 2005. http://dx.doi.org/10.1117/12.633056.
Full text"RqPCRAnalysis: Analysis of Quantitative Real-time PCR Data." In International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2013. http://dx.doi.org/10.5220/0004312002020211.
Full textGaertner, Claudia, Holger Becker, Nadine Hlawatsch, Richard Klemm, Christian Moche, René Sewart, Rainer Frank, and Andreas Willems. "Lab-on-a-chip PCR: real time PCR in miniaturized format for HLA diagnostics." In SPIE Sensing Technology + Applications, edited by Brian M. Cullum and Eric S. McLamore. SPIE, 2014. http://dx.doi.org/10.1117/12.2050233.
Full textReports on the topic "Real-time PCR"
Dilcheva, Valeria, Ivelin Vladov, and Svetlozara Petkova. A Comparative Study of Six Trichinella Species by Real-time PCR Assay. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, January 2018. http://dx.doi.org/10.7546/crabs.2018.01.08.
Full textDilcheva, Valeria, Ivelin Vladov, and Svetlozara Petkova. A Comparative Study of Six Trichinella Species by Real-time PCR Assay. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, January 2018. http://dx.doi.org/10.7546/grabs2018.1.08.
Full textHarris, D. L. Hank, Isabel Turney Harris, James S. Dickson, Stephen Gaul, Brad T. Bosworth, and Lori Feldmann. Quantitative Real-time PCR (qPCR) for the Determination of Salmonella Levels in Lairage. Ames (Iowa): Iowa State University, January 2009. http://dx.doi.org/10.31274/ans_air-180814-1009.
Full textMcAvin, James C., and Carl J. Mason. Pre-Clinical Testing of a Real-Time PCR Assay for Diahhreal Disease Agent Cryptosporidium. Fort Belvoir, VA: Defense Technical Information Center, May 2014. http://dx.doi.org/10.21236/ada600722.
Full textBonab, Zahra Hojjati, Parisa Mohammadi, Ezzat Asgarani, and Nassim Ghorbanmehr. The Evaluation of Nitrogen Fixation Activity of Soil Cyanobacteria via Reduction Assay and Real-time PCR. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, June 2019. http://dx.doi.org/10.7546/crabs.2019.06.07.
Full textFaris, Gregory W. Development of Laser-Mediated Nanodroplet Real-Time PCR on Circulating Tumor Cells (CTC) by Microfilter Platform. Fort Belvoir, VA: Defense Technical Information Center, June 2015. http://dx.doi.org/10.21236/ada621341.
Full textMcAvin, James C., and Carl J. Mason. Norovirus Real Time RT-PCR Detection Technology Transition to the Joint Biological Identification and Diagnosis System (JBAIDS). Fort Belvoir, VA: Defense Technical Information Center, September 2012. http://dx.doi.org/10.21236/ada568257.
Full textMcAvin, James C., and Carl J. Mason. Pre-Clinical Testing of Real-Time PCR Assays for Diarrheal Disease Agents of Genera Escherichia and Shigella. Fort Belvoir, VA: Defense Technical Information Center, May 2014. http://dx.doi.org/10.21236/ada600976.
Full textHutchison, Janine R., Gregory F. Piepel, Brett G. Amidan, Michael A. Sydor, and Brooke L. Deatherage Kaiser. False Negative Rates of a Macrofoam-Swab Sampling Method with Low Surface Concentrations of Two Bacillus anthracis Surrogates via Real-Time PCR. Office of Scientific and Technical Information (OSTI), May 2015. http://dx.doi.org/10.2172/1186982.
Full textHutchison, Janine R., Gregory F. Piepel, Brett G. Amidan, Michael A. Sydor, and Brooke L. D. Kaiser. False Negative Rates of a Macrofoam-Swab Sampling Method with Low Surface Concentrations of Two Bacillus anthracis Surrogates via Real-Time PCR. Office of Scientific and Technical Information (OSTI), June 2016. http://dx.doi.org/10.2172/1260869.
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