Journal articles: 'Rats Infertility'

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Harishankar, Nemani, Punjal Ravinder, K. Madhavan Nair, and Nappanveettil Giridharan. "Infertility in WNIN Obese Mutant Rats—Causes?" ISRN Endocrinology 2011 (December 15, 2011): 1–7. http://dx.doi.org/10.5402/2011/863403.

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We are maintaining two obese mutant rat strains (WNIN/Ob and WNIN/GR-Ob) in our animal facility since 1997. These rat colonies are perpetuated by crossing heterozygous littermates, since the obese phenotypes of both genders turned out be infertile. The present study revealed the reasons for this infertility. The male obese rats, though appeared normal in terms of sperm count, sperm motility and testis histology, however found wanting in terms of libido. This appeared to be due to low circulating testosterone levels seen in these animals, which should also account for low testis and accessory gland weights seen in them. The females exhibited delayed puberty, in terms of days taken for opening of vagina, irregular oestrus cycles and had small ovaries and short and stumpy uterine horns. The FSH peak observed in control lean animals during oestrus stage of the sexual cycle and also the E2 peak of normal oestrus cycle was conspicuously absent in these animals. They also showed elevated levels of progesterone throughout the sexual cycle. Thus the infertility seen in these mutants could be attributed to their abnormal gonadosteroid levels and the resulting anatomical and physiological defects.

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Colluoglu, Cagdas, Serdar Balci, Betul Gundogdu, Taha Abdulkadir Coban, Seval Bulut, and Halis Suleyman. "Effect of thymoquinone on cyclophosphamide-induced oxidative and inflammatory ovarian damage in rat." Bangladesh Journal of Pharmacology 17, no. 3 (September 6, 2022): 72–78. http://dx.doi.org/10.3329/bjp.v17i3.59418.

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This study intends to investigate the effect of thymoquinone against possible ovarian damage and infertility caused by cyclophosphamide in female rats. The rats in the experiment (n=36) were separated into three groups; cyclophosphamide administered, thymoquinone plus cyclophosphamide administered and healthy control. At the end of 30 days of administration, 6 rats from each group were killed and the biochemical and histopathological examina-tions were performed on the ovarian tissues. The remaining animals were kept in the same cage with mature male rats for two months. Infertility developed in 83% of cyclophosphamide group animals. Thymoquinone attenuated cyclophosphamide-related oxidative and inflammatory ovarian damage and reduced the development of infertility. The outcomes suggest that thymoquinone may be useful in treating infertility due to oxidative and inflammatory ovarian damage.

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Gupta, Vijayalaxmi, Sheri A. Hild, Sudhakar R. Jakkaraj, Erick J. Carlson, Henry L. Wong, C. Leigh Allen, Gunda I. Georg, and Joseph S. Tash. "N-Butyldeoxygalactonojirimycin Induces Reversible Infertility in Male CD Rats." International Journal of Molecular Sciences 21, no. 1 (December 31, 2019): 301. http://dx.doi.org/10.3390/ijms21010301.

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This study shows for the first time that an iminosugar exerts anti-spermiogenic effect, inducing reversible infertility in a species that is not related to C57BL/6 male mice. In CD rats, N-butyldeoxygalactonojirimycin (NB-DGJ) caused reversible infertility at 150 mg/kg/day when administered daily as single oral dose. NB-DGJ inhibited CD rat-derived testicular β-glucosidase 2 (GBA2) activity at 10 µM but did not inhibit CD rat-derived testicular ceramide-specific glucosyltransferase (CGT) at doses up to 1000 µM. Pharmacokinetic studies revealed that sufficient plasma levels of NB-DGJ (50 µM) were achieved to inhibit the enzyme. Fertility was blocked after 35 days of treatment and reversed one week after termination of treatment. The rapid return of fertility indicates that the major effect of NB-DGJ may be epididymal rather than testicular. Collectively, our in vitro and in vivo studies in rats suggest that iminosugars should continue to be pursued as potential lead compounds for development of oral, non-hormonal male contraceptives. The study also adds evidence that GBA2, and not CGT, is the major target for the contraceptive effect of iminosugars.

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Altuner, Durdu, Mine Gulaboglu, Omer Erkan Yapca, and Nihal Cetin. "The Effect of Mirtazapine on Cisplatin-Induced Oxidative Damage and Infertility in Rat Ovaries." Scientific World Journal 2013 (2013): 1–6. http://dx.doi.org/10.1155/2013/327240.

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Cisplatin causes infertility due to ovarian toxicity. The toxicity mechanism is unknown, but evidence suggests oxidative stress. In this study, the effect of mirtazapine on cisplatin-induced infertility and oxidative stress in rats was investigated. 64 female rats were divided into 4 groups of 16. Except for the controls that received physiologic saline only, all were administered with cisplatin (5 mg/kg i.p.) and mirtazapine (15 mg/kg p.o.) or mirtazapine (30 mg/kg p.o.) for 10 days. After this period, six rats from each group were randomly selected, and malondialdehyde (MDA), myeloperoxidase (MPO), nitric oxide (NO), total gluthatione (tGSH), gluthatione peroxidase (GPx), superoxide dismutase (SOD), and 8-hydroxy-2 deoxyguanine (8-OH Gua) levels were measured in their ovarian tissues. Reproductive functions of the remaining rats were examined for 6 months. The MDA, MPO, NO groups and 8-OH Gua levels were higher in the cisplatin-treated groups than the controls, which was not observed in the mirtazapine and cisplatin groups. GSH, GPx, and SOD levels were reduced by cisplatin, which was prevented by mirtazapine. Cisplatin caused infertility by 70%. The infertility rates were, respectively, 40% and 10% for the 15 and 30 mg/kg mirtazapine administered groups. In conclusion, oxidative stress induced by cisplatin in the rat ovary tissue causes infertility in the female rats. Mirtazapine reverses this in a dose-dependent manner.

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Al-Alami, Zina M., Ziad A. Shraideh, and Mutasem O. Taha. "Rosmarinic acid reverses the effects of metronidazole-induced infertility in male albino rats." Reproduction, Fertility and Development 29, no. 10 (2017): 1910. http://dx.doi.org/10.1071/rd16174.

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Rosmarinic acid (RA) is a natural antioxidant that has many biological activities. In the present study we investigated the potential of RA to reverse the negative effects of the widely used antibiotic and antiprotozoal agent metronidazole (MTZ), which is known to induce reversible male infertility. Two doses of RA (5 and 15 mg kg–1) were studied in sexually mature rats with and without MTZ-induced infertility. Rats were intraperitoneally injected with 5 mg kg–1 RA or 15 mg kg–1 RA (in distilled water) and, 45 min later, they were intraperitoneally injected with 40 mg kg–1 MTZ (in distilled water). Cauda epididymidal sperm suspensions were used to assess sperm count, motility and morphology. Histological and ultrastructural studies were performed on the testes and cauda epididymidis. In rats in which infertility was not induced, neither dose of RA affected the parameters assessed. However, in sexually mature rats in which infertility was induced by 40 mg kg–1 MTZ, RA at both 5 and 15 mg kg–1 ameliorated the damaging effects of MTZ on final bodyweight (30 days later), sperm motility and morphology. Only 5 mg kg–1 RA, and not 15 mg kg–1 RA, improved the harmful effects of MTZ on the sperm count and testis ultrastructure. The findings of the present study have considerable clinical implications and suggest a possible use for RA to reverse the negative effects of MTZ on male fertility, the male reproductive system and spermatogenesis.

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Minabe, Shiori, Kinuyo Iwata, and Hitoshi Ozawa. "Effects of Diet-Induced Obesity on Hypothalamic Kisspeptin-Neurokinin-Dynorphin (KNDy) Neurons and Luteinizing Hormone Secretion in Sex Hormone-Primed Male and Female Rats." Journal of the Endocrine Society 5, Supplement_1 (May 1, 2021): A537. http://dx.doi.org/10.1210/jendso/bvab048.1094.

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Abstract Metabolic stress resulting from a nutrient excess causes infertility in both sexes. Kisspeptin-neurokinin B-dynorphin (KNDy) neurons in the arcuate nucleus (ARC) have been suggested to be key players in reproduction via direct stimulation of gonadotropin-releasing hormone (GnRH) and subsequent gonadotropin release in mammalian species. In this study, we investigated the sex differences in the effects of a high-fat diet (HFD) on KNDy-associated gene expression in the ARC to determine the pathogenic mechanism underlying obesity-induced infertility. Wistar-Imamichi strain male and female rats (7 weeks of age) were fed either a standard diet (10% calories from fat) or high-fat diet (45% calories from fat) for 4 months. In male rats, the HFD caused a significant suppression of Kiss1(encoding kisspeptin), Tac3(encoding neurokinin B), and Pdyn(encoding dynorphin A) gene expression in the ARC, resulting in a decrease in plasma luteinizing hormone (LH) levels. In female rats, 58% of the HFD-fed female rats exhibited irregular estrous cycles, while the other rats showed regular cycles. LH pulses were found, and the numbers of ARC Kiss1-,Tac3-, and Pdyn-expressing cells were high in control animals and almost allHFD-fed female rats, but two out of 10 rats showed profound HFD-induced suppression of LH pulse frequency and reduction in these cells. No statistical differences in LH secretion or ARC KNDy gene expression were observed between HFD-fed and control female rats. Additionally, the number of Gnrh1-expressing cells in the preoptic area was comparable between the groups in both sexes. Our findings revealed that HFD-fed male rats showed KNDy-dependent infertility, while irregular menstruation was mainly induced by KNDy-independent pathways during the incipient stage of obese infertility in female rats. Taken together, hypothalamic kisspeptin neurons in male rats may be susceptible to HFD-induced obesity compared with those in female rats.

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Oridupa, O. A., N. O. Ovwighose, O. O. Aina, and A. B. Saba. "Reversal of Diabetic Complications in Andrology Parameters of Alloxan-Induced Diabetic Male Wistar Rats Treated with Cleome rutidosperma Leaves." Folia Veterinaria 64, no. 1 (March 1, 2020): 19–26. http://dx.doi.org/10.2478/fv-2020-0003.

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AbstractMale infertility is one of the complications associated with diabetes mellitus. This study reported the effect of managing experimentally-induced diabetes in male Wistar rats with Cleome rutidosperma leaf extract at doses of 100 mg.kg−1, 200 mg.kg−1 or 400 mg.kg−1 for 14 days. Further deterioration or amelioration of reproductive derangement was assessed by changes in sperm morphology, sperm characteristics, and testicular histo-pathology. Andrology profile of diabetic untreated rats showed significant (P < 0.05) derangements compared to normoglycaemic rats. The profile of diabetic rats treated with the extract of C. rutidosperma, especially at the 200 mg.kg−1 dose showed a significant decrease in abnormal sperm cells, increases in sperm motility, live/ dead ratio and count compared to the untreated diabetic rats. Histopathology showed spermatogenic arrest and degeneration of Sertoli cells in the diabetic untreated rats, but was restored in the C. rutidosperma treated rats. This study showed evidence of infertility associated with diabetes and the ameliorative ability of C. rutidosperma on infertility demonstrated by improved andrological parameters. Traditional management of diabetes with C. rutidosperma leaves should therefore be encouraged in both humans and other animals, especially males with reproductive intentions.

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Jin, Zi-Run, Ya-Lei Cao, Zhi-Chao Luo, Qian-Cheng Zhao, Yu Xi, Jia-Ming Weng, Zhe Zhang, and Hui Jiang. "Therapeutic Effects of Xianlu Oral Solution on Rats with Oligoasthenozoospermia through Alleviating Apoptosis and Oxidative Stress." Evidence-Based Complementary and Alternative Medicine 2022 (June 18, 2022): 1–11. http://dx.doi.org/10.1155/2022/1269530.

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Idiopathic oligoasthenozoospermia (iOAZS) is one of the major causes of male infertility, and the ideal therapies for iOAZS have not been established yet. Traditional Chinese medicine (TCM), including Xianlu oral solution (XL), has been widely used as an adjunct treatment for male infertility in the clinic. However, the underlying mechanisms of XL treatment on iOAZS are still not known. Here, we found that XL treatment has therapeutic effects on ornidazole (ORN)-induced OAZS model rats through the amelioration of testis tissues spermatogenesis and the improvement of sperm concentration and motility. Moreover, XL treatment ameliorated the serum hormone levels, mitochondrial membrane potential, apoptosis status, and oxidative stress status in the testis tissues of iOAZS model rats. These findings identify a potential mechanism underlying the therapeutic effects of Xianlu oral solution on iOAZS, and Xianlu oral solution may be used as a traditional Chinese medicine (TCM) therapy for male infertility caused by iOAZS in clinical practice.

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Woodside, Barbara, Alfonso Abizaid, and Shelina Jafferali. "Effect of acute food deprivation on lactational infertility in rats is reduced by leptin administration." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 274, no. 6 (June 1, 1998): R1653—R1658. http://dx.doi.org/10.1152/ajpregu.1998.274.6.r1653.

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The goals of these experiments were to determine whether lactational anestrus would be prolonged by a 48-h fast at days 13 and 14 postpartum (pp) and, if so, to determine whether this effect could be reversed by treatment with the Ob protein leptin. We found that food deprivation on days 13 and 14 pp prolonged lactational infertility by 7 days and that the nutritional experience of both the dam and her litter contributed to this effect. Leptin administration (2.5 mg ⋅ kg−1 ⋅ day−1) during food deprivation was sufficient to reduce the length of lactational infertility compared with vehicle-treated food-deprived rats ( P < 0.05). Similar leptin treatment in ad libitum-fed animals reduced food intake ( P < 0.05) and litter growth ( P < 0.05) but had no statistically significant effect on maternal weight gain or length of lactational infertility. Food-deprived lactating animals had lower circulating leptin levels than ad libitum-fed lactating animals on day 15 pp ( P < 0.05), as determined by RIA. Levels in nonlactating rats were higher than in either lactating group ( P < 0.05).

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Al-Rawaf, Hadeel A., Sami A. Gabr, and Ahmad H. Alghadir. "The Potential Role of Circulating MicroRNAs in Male Rat Infertility Treated with Kaempferia parviflora." Evidence-Based Complementary and Alternative Medicine 2021 (December 17, 2021): 1–14. http://dx.doi.org/10.1155/2021/9622494.

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Background. Therapeutic strategies based on herbal plants and diets containing sufficient amounts of antioxidants and essential vitamins are very important factors in treating reproduction and male infertility worldwide. Thus, the aim of this study was to investigate the potential effects of Kaempferia parviflora (KP) on the role of some microRNAs in treated and nontreated infertile rats. In addition, the correlation of expressed microRNAs with sperm count, sperm motility, and sperm viability was identified. The probable use of these microRNAs as a diagnostic marker for predicting the clinical response of infertility to the treatment with KP was also achieved. Methods. In the present study, the potential effects of Kaempferia parviflora (KP) at different doses (140, 280, and 420 mg/kg) for six weeks on male rats with subinfertility were explored. In addition, the effect of KP on the expression of circulating microRNAs and its correlation with the parameters of sexual infertility was identified by performing both in vitro and in vivo assays. In vitro antioxidant activity, sperm functional analysis, serum testosterone, and expression of circulating microRNAs were conducted using colorimetric, ELISA, and real-time RT-PCR analysis, respectively. Results. Kaempferia parviflora (KP) at nontoxic doses of 140–420 mg/kg/day for six weeks significantly improved serum testosterone and epididymal sperm parameters (sperm count, motility, and sperm viability), increased testicular weight, and provided a reduction in the percentage of abnormal spermatozoon in infertile male rats. The expression of miR-328 and miR-19b significantly decreased, and miR-34 significantly increased in infertile rats treated with KP compared to infertile nontreated rats. After six weeks of KP therapy, the change in the expression levels of miRNAs was correlated positively with higher levels of serum testosterone and the measures of epididymal sperm parameters. The respective area under the receiver operating characteristic curve (AUC-ROC) was applied to predict the potential use of miR-328, miR-19b, and miR-34 in the diagnosis of male infertility in treated and nontreated infertile male rats. The data showed that AUC cutoff values of 0.91 for miR-328, 0.89 for miR-19b, and 0.86 for miR34 were the best estimated values for the clinical diagnosis of male rats with infertility. In rats treated with KP for six weeks, AUC cutoff values of 0.76 for miR-328, 0.79 for miR-19b, and 0.81 for miR-34 were the best cutoff values reported for the clinical response of infertility to KP therapy after six weeks. Conclusions. In this study, the improvement of male infertility might proceed via antioxidant and antiapoptotic pathways, which significantly improve spermatogenesis and aphrodisiac properties of males. In addition, the expression of miRNAs, miR-328, miR-34, and miR-19b, in KP-treated and nontreated infertile rats significantly correlated with increased serum testosterone levels and epididymal sperm parameters as well. MicroRNAs, miR-328, miR-34, and miR-19b, might be related to oxidative and apoptotic pathways that proceeded in spermatogenesis. Thus, the use of miRNAs could have a role as diagnostic, therapeutic, and predictive markers for assessing the clinical response of Kaempferia parviflora treatment for six weeks. This may have potential applications in the therapeutic strategies based on herbal plants for male infertility. However, in subsequent studies, the genetic regulatory mechanisms of the expressed miRNAs should be fully characterized.

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ADESIJI, Yemisi Olukemi, Benjamin Obukowho EMIKPE, and Ahmed Bayo OPALEKUNDE. "Histopathological and functional changes in the testicles of albino rats experimentally infected with Arcobacter butzleri." Acta medica Lituanica 18, no. 3 (July 1, 2011): 127–31. http://dx.doi.org/10.6001/actamedica.v18i3.1826.

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Background. Arcobacter is an emerging zoonotic enteropathogen associated with spontaneous abortion, diarrhea and mastitis in domestic animals. Materials and methods. Thirty male pathogen-free albino rats were infected with a single challenge of Arcobacter butzleri (108 cfu/ml) isolated from the stool of healthy pigs with the aim of investigating the effects of A. butzleri on rat testicular histology and spermatogenesis. Results. In previously healthy male albino rats, A. butzleri caused testicular degeneration associated with reduced sperm count and motility. Conclusions. The result of this study suggests that A. butzleri produces testicular degeneration and the associated disruption of spermatogenesis in albino rats; hence, its infertility potential in livestock industry and its economic importance should be further investigated. Keywords: Arcobacter butzleri, testicular degeneration, infertility,pathogenicity, sperm count, albino rats

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Chidrawar, V. R., H. R. Chitme, K. N. Patel, N. J. Patel, V. R. Racharla, N. C. Dhoraji, and K. R. Vadalia. "Effects of Cynodon dactylon on Stress-Induced Infertility in Male Rats." Journal of Young Pharmacists 3, no. 1 (January 2011): 26–35. http://dx.doi.org/10.4103/0975-1483.76416.

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Chen, Yegang. "AB113. The function of miR-210 in varicocele rats with infertility." Translational Andrology and Urology 5, S1 (April 2016): AB113. http://dx.doi.org/10.21037/tau.2016.s113.

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Linder, Ralph E., Rex A. Hess, and Lillian F. Strader. "Testicular toxicity and infertility in male rats treated with 1,3‐dinitrobenzene." Journal of Toxicology and Environmental Health 19, no. 4 (December 1986): 477–89. http://dx.doi.org/10.1080/15287398609530946.

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Larsen, Jens-Jargen, Merete Lykkegaard, and Ole Ladefoged. "Infertility in Rats Induced by 2,5-Hexanedione in Combination with Acetone." Pharmacology & Toxicology 69, no. 1 (July 1991): 43–46. http://dx.doi.org/10.1111/j.1600-0773.1991.tb00407.x.

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Retana-Márquez, Socorro, Lizbeth Juárez-Rojas, Alejandra Ávila-Quintero, Susana Rojas-Maya, Gerardo Perera, Fahiel Casillas, Miguel Betancourt, and Luis Gómez-Quiroz. "Neuroendocrine disruption is associated to infertility in chronically stressed female rats." Reproductive Biology 20, no. 4 (December 2020): 474–83. http://dx.doi.org/10.1016/j.repbio.2020.07.011.

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Omotosho, Ishiaq O., and Temitope O. Olusanya. "Experimental Zinc Supplementation on Reproductive Hormonal Balance in Female Wister Rats in The Management of Secondary Infertility." European Journal of Medical and Health Sciences 4, no. 3 (June 11, 2022): 92–97. http://dx.doi.org/10.24018/ejmed.2022.4.3.1230.

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Several factors including hormonal and essential elements imbalance have been identified as possible causes of secondary infertility. While some studies have suggested that zinc may have the potential of reversing sub-fertility states, the dynamics of such impact is yet to be fully elucidated. This study evaluated the possible outcome of experimental Zn supplementation in ameliorating infertility in induced secondary infertile Wister rats. A total of 30 female rats each weighing averagely 120g were stratified into six groups: Group 1 – Baseline group; Group 2 – animals induced with secondary infertility using Levonorgestrel (LNG); Group 3 - Secondary infertility and administered 10mgZn/Kg bodyweight (LNG + 10mg Zn/kg bodyweight); Group 4 - LNG+50mgZn/kg; Group 5 -LNG+75mgZn/kg; Group 6 - LNG+100mgZn/kg. Blood zinc and reproductive hormones were measured at pre and post treatment phases using standard laboratory methods. Results were analyzed using appropriate statistical methods. A significant up-regulation of zinc in group 6 at the end of the study (pre-test Zn level= 1.39±0.1 mg/l, post-test Zn = 2.74±0.5 mg/l) and a significant dose dependent increase in the post-treatment progesterone levels in the experimental groups fed with graded doses of zinc (Group 4 = 11.34±2.2mg/l, Group 5 = 12.38±1.9mg/l, Group 6 = 15.9±3.6 mg/l) were observed. There was however a significant dose dependent drop in the FSH levels in the animals fed with different doses of zinc post-treatment. There may be a regulatory effect of zinc on the hypothalamic-pituitary-gonadal-axis through modulation of FSH and progesterone levels in Zn supplemented secondary infertile experimental animals.

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Prasad, Mergu, and Satya Prasad Venugopal. "THE ROLE OF ETHANOLIC EXTRACT OF SYZYGIUM CUMINI STEM BARK ON FEMALE REPRODUCTIVE SYSTEM IN WISTAR RATS." International Journal of Anatomy and Research 8, no. 4.2 (December 5, 2020): 7835–40. http://dx.doi.org/10.16965/ijar.2020.239.

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Background: The medicinal plant's role is well established in folklore medicine in India. Among numerous health complications, infertility is a crucial condition that bothers modern society. The traditional practitioners are using various parts of plants to overcome infertility in women. One such plant is Syzygium cumini (stem bark), used by traditional practitioners for pro-fertility in females without the scientific literature endorsing the fact. Material and Methods: Wistar rats approximately six months age and weighing 190-210 g were used for the experimental study. The rats were treated with 500 mg/kg body weight of Syzygium cumini stem bark ethanol extract with a vehicle through oral administration. The ether anaesthesia was given on the day of sacrifice and noted the body weight. The ovaries were carefully dissected, cleaned, weighed and processed for histological studies. Results: The body and ovarian weights were slightly increased in the treated group but not statistically significant. The microscopic structure of the ovary showed a normal histo-architecture. The ovarian corpora lutea number increased and was statistically significant in the treated group when compared to control. Conclusions: So, from the present study, it can be concluded that the ethanolic extract of stem bark of Syzygium cumini brings about the pro-fertility effect on the female reproductive system in Wistar rats. KEY WORDS: Estrous cycle, Hormones, Reproductive system, Syzygium cumini.

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ADELEYE, O. E., N. A. ABOAJAH, A. I. ADELEYE, E. A. O. SOGEBI, F. M. MSHELBWALA, A. S. ADETOMIWA, and J. O. OLUKUNLE. "ANNONA MURICATA LINN. ETHANOLIC LEAF EXTRACT AMELIORATES REPRODUCTIVE COMPLICATIONS IN STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS." Journal of Natural Sciences Engineering and Technology 18, no. 1 (October 6, 2020): 166–75. http://dx.doi.org/10.51406/jnset.v18i1.2040.

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Diabetes mellitus is an endocrine and metabolic disorder of humans and animals characterized by hyperglycemia and low blood insulin levels or insensitivity of target organs to insulin and it’s a major health problem affecting patient’s quality of life due to its many complications. Infertility is one of the major secondary complications in diabetes. Although numerous drugs have been used for intervention studies on diabetes-induced infertility worldwide, there are currently no treatments for diabetes associated infertility in humans. This study was performed to investigate the effects of Annona muricata ethanolic leaf extract (AMELE) on fertility of male diabetic rats and levels of blood glucose. Twenty male Wistar rats (150-200g) were randomly distributed into 4 groups (n=5) treated thus: CTRL (control), DNT, DT1 and DT2 (diabetic, single intraperitoneal injection, streptozotocin, 60 mg/kg). Group DT1 and DT2 received AMELE orally at 100 mg/kg and 200 mg/kg respectively daily for fourteen days. Data were analysed using ANOVA at α0.05. The animals were sacrificed after 2 weeks via thiopental injection and testicular weights were recorded. Fasting blood glucose was determined using a digital glucometer. Sperm count, motility, viability and morphology were assessed microscopically. Testes were histologically evaluated. The results showed that oral administration of AMELE at 100 mg/kg and 200 mg/kg to diabetic male rats for fourteen days significantly decreased blood glucose level and also ameliorated diabetes-induced decreases in sperm functions in streptozotocin-induced diabetic male rats.

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Abizaid, Alfonso, Diana Kyriazis, and Barbara Woodside. "Effects of leptin administration on lactational infertility in food-restricted rats depend on milk delivery." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 286, no. 1 (January 2004): R217—R225. http://dx.doi.org/10.1152/ajpregu.00128.2003.

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Leptin administration has been shown to prevent the disruptive effects of acute food deprivation on reproductive function in cycling females and lactating females. We examined the ability of intracerebroventricular leptin administration to ameliorate the effects of food restriction for the first 2 wk postpartum on length of lactational infertility. Leptin administration did not reduce the effects of food restriction on reproductive function at either time period ( days 8-15 and 15-22 postpartum) or dose (1 and 10 μg/day) administered. Because of the sharp contrast between these results and the ability of leptin to offset the effects of acute food deprivation in lactating rats, the remaining studies investigated the possible causes of this difference. Both central and peripheral leptin administration eliminated food deprivation-induced prolongation of lactational infertility, suggesting that neither route of administration nor dose was a factor. However, we noticed that, whereas chronically food-restricted females continue to deliver milk to their young, acutely food-deprived females do not. To test the hypothesis that the continued energetic drain of milk production and delivery might prevent the ability of exogenous leptin administration to eliminate the effects of undernutrition, leptin was administered to food-restricted, lactating rats prevented from delivering milk. In this situation intracerebroventricular leptin treatment completely eliminated the effects of food restriction on lactational infertility, suggesting that leptin contributes to the maintenance of reproductive function via two pathways: direct binding in the central nervous system and through increasing the availability of oxidizable metabolic fuels.

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Suleiman, Joseph Bagi, Victor Udo Nna, Zaida Zakaria, Zaidatul Akmal Othman, Ainul Bahiyah Abu Bakar, Umar Zayyanu Usman, and Mahaneem Mohamed. "Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats." Reproduction 160, no. 6 (December 2020): 863–72. http://dx.doi.org/10.1530/rep-20-0381.

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Obesity and its accompanying complications predispose to abnormal testicular glucose metabolism, penile erectile dysfunction and subfertility. This study examined the potentials of orlistat in attenuating erectile dysfunction and fertility decline in high-fat diet (HFD)-induced obesity in male rats. Eighteen adult male Sprague–Dawley rats whose weights were between 250 and 300 g were divided into three groups (n = 6/group) namely: normal control (NC), HFD and HFD + orlistat (10 mg/kg body weight/day co-administered for 12 weeks) (HFD+O). During the 11th and 12th week, mating behaviour and fertility parameters were evaluated, and parameters of glucose metabolism were assessed at the end of the 12th week. Orlistat increased testicular mRNA levels of glucose transporters (Glut1 and Glut3), monocarboxylate transporters (Mct2 and Mct4) and lactate dehydrogenase type C (Ldhc), decreased intratesticular lactate and glucose levels, and LDH activity in obese rats. Furthermore, orlistat increased superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) activities, and total antioxidant capacity (TAC), but decreased malondialdehyde level in the penis of obese rats. Similarly, orlistat improved penile cGMP level, sexual behaviour and fertility outcome in obese rats. Penile cGMP level correlated positively with total mounts and intromissions but correlated negatively with mount/intromission ratio. Orlistat improves fertility potential in obese state by targeting testicular lactate metabolism, penile oxidative stress and sexual behaviour in rats. Therefore, orlistat shows a promising protective effect and may preserve the fertility potential of obese men.

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Deldar Abad Paskeh, Mahshid, Nahid Babaei, Maliheh Entezari, Mehrdad Hashemi, and Abbas Doosti. "Protective Effects of Coenzyme Q10 Along with Fe2O3 Nanoparticles On Sperm Parameters in Rats with Scrotal Hyperthermia." Galen Medical Journal 11 (May 8, 2022): e2046. http://dx.doi.org/10.31661/gmj.v11i.2046.

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Background: One of the most important factors in reducing the birth rate is male infertility, and one of the main reasons for male infertility is scrotal hyperthermia (SH). Therefore, this study aimed to investigate the protective effect of coenzyme Q10 (CoQ10) along with Fe2O3 nanoparticles on semen parameters in rats with SH. Materials and Methods: Forty-eight adult male Wistar rats were divided into eight groups: healthy control, control group receiving Fe2O3 nanoparticles, control group receiving CoQ10, control group receiving Fe2O3 nanoparticles plus CoQ10, SH group, SH group receiving CoQ10, SH group receiving Fe2O3 nanoparticle, and SH group receiving Fe2O3 nanoparticles plus CoQ10. After killing rats, semen was collected from epididymal tissue, and parameters such as sperm viability, motility, concentration, and morphology were studied. Results: SH significantly reduced sperm concentration, motility, and viability, as well as altering sperm morphology in rats. Nevertheless, CoQ10 strongly improved sperm parameters in SH rats. Fe2O3 nanoparticles led to a sharp decrease in sperm parameters; however, during the simultaneous administration of Fe2O3 nanoparticles with CoQ10, improvement in sperm parameters was seen in the SH rats. Conclusion: Our findings suggest that CoQ10, along with Fe2O3 nanoparticles, has a protective effect against spermatogenic cell death induced by SH. Thus, green synthesis of nanoparticles with the administration of antioxidants, including CoQ10 is recommended for the treatment of SH. [GMJ.2022;11:e2046]

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Hassan, Amal I., and Sally S. Alam. "Evaluation of mesenchymal stem cells in treatment of infertility in male rats." Stem Cell Research & Therapy 5, no. 6 (2014): 131. http://dx.doi.org/10.1186/scrt521.

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Jarvi, Keith. "Hairless or fertile? Finasteride leads to epididymal changes and infertility in rats." Fertility and Sterility 97, no. 6 (June 2012): 1298. http://dx.doi.org/10.1016/j.fertnstert.2012.04.016.

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Abdul-Majeed, Shakila, Blair Mell, Surya M. Nauli, and Bina Joe. "Cryptorchidism and Infertility in Rats with Targeted Disruption of the Adamts16 Locus." PLoS ONE 9, no. 7 (July 1, 2014): e100967. http://dx.doi.org/10.1371/journal.pone.0100967.

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Hirsch, Kenneth S., Elizabeth R. Adams, Donald G. Hoffman, Janet K. Markham, and Norris V. Owen. "Studies to elucidate the mechanism of fenarimol-induced infertility in male rats." Toxicology and Applied Pharmacology 86, no. 3 (December 1986): 391–99. http://dx.doi.org/10.1016/0041-008x(86)90366-2.

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Ekapatria, Christofani, Wiryawan Permadi, Maringan Diapari Lumban Tobing, Meita Dhamayanti, and Johanes Cornelius Mose. "The use of vinylcyclohexene diepoxide to create diminish ovarian reserve model on rats." F1000Research 11 (October 17, 2022): 1181. http://dx.doi.org/10.12688/f1000research.125617.1.

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Background: Infertility remains a significant issue in the world of health. Now Assisted Reproductive Technology (ART) is widely used to help couples with infertility. In ART diminish ovarian reserve patients have a very low success rate of pregnancy. To help patients with Diminished Ovarian Reserve (DOR), research is conducted using an animal model. vinylcychlohexene diepoxide (VCD) has ovotoxic effects. This study looked at the use of VCD in inducing targeted DOR conditions in animals (rats), create a model that could be used for future animal studies in infertility. Methods: Adult female Rattus norvegicus were used in this study. All were given VCD injections at 80 mg/kg intraperitoneally for 15 days. An examination of Follicle Stimulating Hormone (FSH) levels will be carried out on days 0,3,5,7, and 15. To mimic IVF process, all rats got an injection of 10 IU of Pregnant Mare Serum Gonadotropin (PMSG) and 10 IU human Chorionic Gonadotropin (hCG) to confirm DOR. Ovarectomy was performed, dominant follicles were taken, denudation was carried out, and the oocytes were cultured for 12 hours. Then, oocytes were assessed through a microscope. Results: Starting from day 5 post VCD induction, there was a significant increase in the FSH level in the group of rats that were induced with the administered VCD dose compared to the control group that experienced normal FSH fluctuations. The FSH concentration reached >50%. It was found that the number of degenerated oocytes had an average of 1.11. Oocytes that were in Germinal-Vesicle (GV) stage had an average of 1.89, while in the Metaphase I (M1) stage the average is 2.882. Lastly, the number of oocytes in the Metaphase (MII) stage which is fully matured had an average of 1.117. Conclusions: VCD can become an important tool for future studies that needs an animal model with DOR.

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Zahir, Munazza, Ghazazfar Ali Khan, Summera Aslam, Arva Sarosh, Muhammad Shahzad, and Talha Laique. "Histological Effects of Cotton seed Oil on Ovaries and RTWI among Female Albino Rats: RCT." Pakistan Journal of Medical and Health Sciences 15, no. 10 (October 30, 2021): 2627–28. http://dx.doi.org/10.53350/pjmhs2115102627.

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Background: Concerns over the rising infertility rate among humans is now becoming a global health issue. Aim: To observe the histological changes in ovaries while measuring relative tissue weight indices of all ovaries among different groups receiving cottonseed oil. Study Design: Randomized controlled trial. Methodology: Twenty four adult female albino rats (12 weeks of age) were divided into 04 groups. Group-1 (Control) rats were given orally distilled water @ 400mg (equivalent to 400μl)/kg body weight per day. Group-2, Group-3 & Group-4 rats were given cottonseed oil (treatment groups) @ 400mg (equivalent to 432μl)/kg body weight per day, respectively, for a period of 30 days. Results: Cottonseed oil significantly reduced fertility in terms of follicles and ova in all treatment groups than control having non-significant differences among means of different groups. Conclusion: This study concluded that the crude cottonseed oil significantly affected female rat fertility in terms of reduced number of follicles and ova as shown histologically. However, it did not affect relative tissue weight indices among different groups. Hence, its use in edible products may result in increased women infertility. Keywords: Cottonseed Oil, Relative Tissue Weight Indices and Number of Follicles.

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An, Tian, Hui Fan, Yu F. Liu, Yan Y. Pan, Ying K. Liu, Fang F. Mo, Yu J. Gu, et al. "The difference in expression of long noncoding RNAs in rat semen induced by high-fat diet was associated with metabolic pathways." PeerJ 5 (July 25, 2017): e3518. http://dx.doi.org/10.7717/peerj.3518.

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Background Obesity, a common metabolic disease, is a known cause of male infertility due to its associated health risk. Long noncoding RNAs (lncRNAs) have also been reported to be associated with male reproductive diseases; however, their role in the association between high-fat diet-induced obesity (DIO) and male reproduction remains unclear. Methods We used microarray analysis to compare the expression levels of lncRNAs and mRNAs in the spermatozoa of rats with DIO and normal rats. We selected a few lncRNAs that were obviously up-regulated or down-regulated, and then used RT-PCR to verify the accuracy of their expression. We then performed a functional enrichment analysis of the differentially expressed mRNAs using gene ontology and pathway analysis. Finally, target gene predictive analysis was used to explore the relationship between lncRNAs and mRNAs. Results The results revealed a statistically significant difference in the fasting blood glucose level in rats with DIO and control rats. We found that 973 lncRNAs and 2,994 mRNAs were differentially expressed in the sperm samples of the DIO rats, compared to the controls. GO enrichment analysis revealed 263 biological process terms, 39 cellular component terms, and 40 molecular function terms (p < 0.01) in the differentially expressed mRNAs. The pathway analysis showed that metabolic pathways were most enriched in protein-coding genes. Discussion To the best of our knowledge, this is the first report to show differences in the expression levels of lncRNAs and mRNAs in the sperms of rats with DIO and normal rats, and to determine the expression profile of lncRNAs in the sperm of rats with DIO. Our results have revealed a number of lncRNAs and pathways associated with obesity-induced infertility, including metabolic pathways. These pathways could be new candidates that help cope with and investigate the mechanisms behind the progression of obesity-induced male infertility.

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Anbu, A. Sujima, and P. Venkatachalam. "Biological macromolecule cross linked TPP–chitosan complex: a novel nanohybrid for improved ovulatory activity against PCOS treatment in female rats." RSC Advances 6, no. 97 (2016): 94301–13. http://dx.doi.org/10.1039/c6ra07228c.

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Xiong, Zhang, Fan, Han, Wu, Liu, Miao, et al. "β-Endorphin Induction by Psychological Stress Promotes Leydig Cell Apoptosis through p38 MAPK Pathway in Male Rats." Cells 8, no. 10 (October 16, 2019): 1265. http://dx.doi.org/10.3390/cells8101265.

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: Psychological stress (PS) disturbs the reproductive endocrine system and promotes male infertility, but the underlying pathogenic mechanisms have not been extensively studied. This study aimed to uncover the mechanisms of PS-induced male reproductive related abnormalities subjected to a ‘terrified sound’ exposure. Male rats subjected to PS displayed slow growth, decreased sperm quality, abnormal levels of the reproductive endocrine hormones, decreased expression of the reproductive-related proteins androgen-binding protein (ABP) and bromodomain-containing protein (BRDT), increased apoptosis in the testis, and accompanied by elevated levels of β-endorphin (β-EP). These effects were reversed by naloxone. Furthermore, PS-induced β-EP could promote mu opioid receptor (MOR) activation and ensure intracellular p38 MAPK phosphorylation and then lead to Leydig cells (LCs) apoptosis. The current result showed that β-EP was a key factor to PS-induced male infertility.

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Oyeniran, David Anuoluwapo, Abdulfatai Olakunle Ojewale, Peter Imoni Jewo, Ebenezer Adeola Ashamu, Olajumoke Oluwaseun Adeniyi, and Sunday Aderemi Adelakun. "Infertility: A product of smoke emanating from Transfluthrin coated insecticide paper (TCIP)." Toxicology Research and Application 5 (January 1, 2021): 239784732110254. http://dx.doi.org/10.1177/23978473211025467.

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Transfluthrin is a fast-acting insecticide used in household and hygiene products, mainly against flying insects, such as mosquito and flies, and in agriculture material pests. Its uses in these areas have not been without health risk to humans and the ecosystem. Therefore, the current study was designed to investigate the effect of smoke emanating from Transfluthrin Coated Insecticide Paper (TCIP) on adult Wistar rats’ testicular functions. The rats were grouped into three. Group A was exposed to normal environmental air. Group B and C rats were exposed via whole-body inhalation to smoke emanating from 6 g and 12 g of TCIP every day for 8 weeks. The entire groups contained eight rats each. At the end of the exposure, body and organs weight, semen analysis, biochemical assay and histological examination were evaluated and determined. The results show that the exposure significantly altered the testicular cytoarchitecture, sperm quality, hormonal profile and oxidative parameters with an increase in exposure to TCIP. This study revealed that exposure to transfluthrin is detrimental to the reproductive functions of male rats.

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Iftikhar, Ayesha, Muhammad Furqan Akhtar, Ammara Saleem, Amjad Riaz, Mehrukh Zehravi, Md Habibur Rahman, and Ghulam Md Ashraf. "Comparative Potential of Zinc Sulfate, L-Carnitine, Lycopene, and Coenzyme Q10 on Cadmium-Induced Male Infertility." International Journal of Endocrinology 2022 (June 30, 2022): 1–13. http://dx.doi.org/10.1155/2022/6266613.

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The human exposure to toxic chemicals and heavy metals is one of the main predisposing factors contributing to male infertility. Acute exposure to cadmium chloride results in testicular damage and infertility. The purpose of the present study was to investigate and compare the curative effect of coenzyme Q10 (CoQ10), lycopene, L-carnitine (LC), and zinc sulfate against the cadmium-induced infertility in male Wistar rats. Cadmium chloride (0.4 mg/kg/day) was orally administered to rats for three consecutive days. Then, oral administration of different treatments (i.e., LC 100 mg/kg, CoQ10 20 mg/kg, lycopene 4 mg/kg, zinc sulfate 6 mg/kg, and a combination LC-CoQ10 at 500/50 mg/kg) was carried out for 30 days. The impact of different treatments on semen parameters, such as sperm count and motility, testicular antioxidants, and serum testosterone, was determined. Furthermore, the morphology of epididymis sperms and histopathology of rat testes were also assessed. Cadmium exposure decreased the sperm count, progressive sperm motility, testosterone, superoxide dismutase (SOD), and catalase and reduced glutathione (GSH). It also caused banana sperm tail, bent sperm head, vacuolization of seminiferous tubules, and oligospermia in rat testes. All treatments with nutraceuticals improved sperm count, sperm morphology, serum testosterone, vacuolization of seminiferous tubules, and oligospermia in diseased rats. Treatment with lycopene, LC, and LC-CoQ10 improved progressive sperm motility and other parameters and increased SOD, GSH, and CAT in the rat testes. CoQ10 also increased SOD activity in rat testes’ tissue homogenates. It is concluded from the current study that all nutraceuticals partially improved reproductive toxicity of cadmium. The administration of lycopene and a high-dose combination of LC-CoQ10 were more efficacious in treating cadmium-induced infertility than other treatments. Treatment of cadmium-exposed rats with lycopene, LC, CoQ10, and LC-CoQ10 improved sperm count and motility through reduction of testicular oxidative stress and improving serum testosterone.

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Falana, B. A., W. M. Adekeye, and S. O. Adeleke. "Amelioration of lead induced changes in the testes of sprague-dawley rats, by methanol extract of Telfaria occidentalis." Anatomy Journal of Africa 8, no. 2 (September 13, 2019): 1606–13. http://dx.doi.org/10.4314/aja.v8i2.189705.

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The prevalence of infertility due to industrial, environmental and pharmaceutical toxins induced by lead in Nigeria and in developing countries is alarming. The use of medicinal plants as fertility enhancer in human is now in the increase because of the shifting of attention from synthetic drugs to natural plants. Thus, this study investigated the effect of methanolic extract of Telfairia occidentalis on (Pb) lead-induced testicular damage in adult male Sprague-Dawley rats. Twenty-four (24) Sprague dawley (SD) rats with average weight of 130g were randomly divided into four groups of six animals each. Group A served as the control group administered normal saline. Group B received 75mg/kg body weight of lead (Pb) as Lead nitrate only. Group C received 75mg/kg body weight of lead (Pb) and 300mg/kg body weight of methanolic extract of Telfairia Occidentalis, while Group D receive 300mg/kg body weight of methanolic extract of Telfairia Occidentalis only. Administration was via oral canula and the animals were sacrificed on 15th day. Blood samples were obtained via the left ocular sinus for haematological and hormonal studies while the testes and epididymis were removed and fixed in Bouin’s fluid for histological analysis. Administration of Telfairia Occidentalis improves testicular testosterone and PCV levels and also restored the histoarchitecture of the testes in SD rats. It is therefore suggested that the antioxidant potential of this wonder plant may have beneficial effects in treating male factor infertility as shown in this present study.Keywords: Male infertility, Telfairia occidentalis, Lead (Pb), Antioxidants

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Kanellopoulos, Dimitrios, Dimitra Karagianni, Vasilios Pergialiotis, Grigorios Patsouras, Konstantinos Patsouras, Nikolaos Nikiteas, Andreas C. Lazaris, and Dimitrios Iliopoulos. "The interplay between endometriosis and fertility in rats: a systematic review." Journal of Medicine and Life 15, no. 6 (June 2022): 742–46. http://dx.doi.org/10.25122/jml-2021-0329.

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For the last decades, endometriosis has been a major gynecological problem and a significant cause of infertility for women worldwide. It is estimated that the disease affects about 10–15% of all women of reproductive age and 70% of women suffering from chronic pelvic pain. At the same time, the incidence is about 40–60% in women with dysmenorrhea and 20–30% in women with subfertility. Despite the high percentage of affected women, endometriosis is still characterized by insufficient knowledge of the pathogenic processes, leading to the development and continuity of the disease. For this reason, there is a significant need for insight and understanding of the pathogenesis of endometriosis. This systematic review aims to present the latest data on the use of rats in endometriosis research and to explore how fertility is affected in rats with endometriosis. The methodology included a review of the available publications retrieved by a search in various scientific databases, such as PubMed, Scopus, Medline, and Google Scholar. The initial search generated 30 titles, with 10 articles fulfilling the inclusion criteria. In conclusion, several surgical techniques have been proposed to induce endometriosis, mainly using rats as the appropriate animal model. Studies in rats showed that endometriosis causes infertility and that pregnancy rates are lower for rats with endometriosis than those without endometriosis. In addition, rats with endometriosis have significant abnormalities in the structure of their oocytes as well as in the development of their embryos (genetic abnormalities).

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Garcia, Patrícia Carvalho, Renata Carolina Piffer, Daniela Cristina Cecatto Gerardin, Michele Kimie Sankako, Rodrigo Otávio Alves de Lima, and Oduvaldo Câmara Marques Pereira. "Could zinc prevent reproductive alterations caused by cigarette smoke in male rats?" Reproduction, Fertility and Development 24, no. 4 (2012): 559. http://dx.doi.org/10.1071/rd10063.

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The aim of the present study was to evaluate the effects of zinc on fertility through semen parameters, testosterone level and oxidative DNA damage to spermatozoa of rats exposed to cigarette smoke. Male Wistar rats (60 days old) were divided into four groups (n = 10 per group): control, cigarette-smoking (20 cigarettes per day), zinc (zinc chloride 20 mg kg–1 day–1) and zinc plus cigarette-smoking (zinc chloride 20 mg kg–1 day–1; 20 cigarettes per day). The treatment was applied for nine weeks and the following parameters were analysed: bodyweight, wet weights of the reproductive organs and the adrenal gland, plasma testosterone concentration, testicular function (seminal analysis and daily sperm production) and sperm DNA oxidative damage. The exposure to cigarette smoke decreased testosterone concentration, the percentage of normal morphology and the motility of spermatozoa. In addition, this exposure increased sperm DNA oxidative damage. Zinc treatment protected against the toxic damage that smoking caused to spermatozoa. This study showed a correlation between smoking and possible male infertility and subfertility, and also that the majority of smoking-induced changes in spermatozoa were prevented by zinc treatment. In conclusion, zinc, an antioxidant and stimulant of cell division, can be indicated as a promising treatment in men with infertility caused by the toxic components of cigarette smoke.

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H. Bakr, El-Sayed, and Mona A. El-Yamany. "Chemical and Nutraceutical Studies on Infertility of Albino Rats Induced by Cadmium Chloride." Pakistan Journal of Biological Sciences 23, no. 10 (September 15, 2020): 1245–52. http://dx.doi.org/10.3923/pjbs.2020.1245.1252.

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Prakash, Ramakrishnan, Rajulu Dheeraj, Palayan Muralidharan, Krishnan Karthickeyan, Raji Vijayakumar, and Thiraviam Purushoth Prabu. "Aphrodisiac Activity of Cocos nucifera Attentuates Ceric Sulphate Induced Infertility in Male Rats." Pharmacology, Toxicology and Biomedical Reports 1, no. 2 (May 22, 2015): 62–68. http://dx.doi.org/10.5530/ptb.1.2.5.

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Erkan Yapca, Omer, Bunyamin Borekci, Mehmet Ibrahim Turan, Mine Gulapoglu, and Suleyman Salman. "The effect of mirtazapine on methotrexate-induced oxidative damage and infertility in rats." ScienceAsia 40, no. 2 (2014): 152. http://dx.doi.org/10.2306/scienceasia1513-1874.2014.40.152.

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Al-Shahari, Eman Abdulqader, and Attalla Farag El-k. "Potential Effect of Grape Seeds Extract Against Monosodium Glutamate Induced Infertility in Rats." International Journal of Pharmacology 15, no. 2 (February 1, 2019): 287–94. http://dx.doi.org/10.3923/ijp.2019.287.294.

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SHARPE, KATHY L., MARIA C. BERTERO, BENJAMIN P. LYON, KEN N. MUSE, and MICHAEL W. VERNON. "Follicular Atresia and Infertility in Rats Treated with a Gonadotropin-Releasing Hormone Antagonist*." Endocrinology 127, no. 1 (July 1990): 25–31. http://dx.doi.org/10.1210/endo-127-1-25.

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El-Habibi, El-Sayed Mohamed, Magda Mahmoud El-Komy, and Heba Osama Saad. "Protective Effect of Date Palm Extracts on Cadmium-Induced Infertility in Male Rats." Egyptian Journal of Hospital Medicine 69, no. 4 (October 2017): 2181–90. http://dx.doi.org/10.12816/0041514.

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Akinsomisoye, Olumide Stephen, Gopal Gupta, and Yinusa Raji. "Experimental dapsone administration induces infertility in male Wistar rats: Mechanisms and clinical implications." Pathophysiology 26, no. 3-4 (September 2019): 289–303. http://dx.doi.org/10.1016/j.pathophys.2019.07.004.

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Harada, Miwa, Koji Kishimoto, Tadakazu Furuhashi, Kazuyosi Naito, Yoshifumi Nakashima, Yoshiro Kawaguchi, and Isao Hiraoka. "Infertility Observed in Reproductive Toxicity Study of N-Acetyl-l-Cysteine in Rats." Biology of Reproduction 69, no. 1 (July 1, 2003): 242–47. http://dx.doi.org/10.1095/biolreprod.102.013862.

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Ma, Zhen, Xueling Liu, Haiwang Lu, Haoming Li, Ruizhi Gao, Rong Wen, Zhiping Tang, Haihui Yin, Yun He, and Hong Yang. "The Mechanism and Experimental Validation of Forsythoside A in the Treatment of Male Infertility Were Analyzed Based on Network Pharmacology and Molecular Docking." Evidence-Based Complementary and Alternative Medicine 2022 (October 6, 2022): 1–14. http://dx.doi.org/10.1155/2022/7723358.

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Chinese medicine extracts are currently the hotspot of new drug research and development. Herein, we report the mechanism of action of the traditional Chinese medicine extract Forsythiaside A in the treatment of male infertility and experimental verification. We first obtained 95 intersection genes between the target protein of Forsythiaside A and the target genes of male infertility and screened 13 key genes. In molecular docking, Forsythiaside A can each have a higher total docking score with 12 key genes and have a better combination. These 95 intersection genes are mainly related to biological processes such as response to peptide hormone, response to oxidative stress, and participation in the oxidative stress of the forkhead box O (FoxO) signaling pathway. Therefore, we use ornidazole to induce an experimental model of oligoasthenospermia in rats and use different concentrations of Forsythiaside A to intervene. We proved that the semen quality and superoxide dismutase (SOD) activities of model group rats were significantly lower than those of the blank group, and semen quality and SOD activities of the low-dose group and high-dose group were significantly higher than those of the model group. The malondialdehyde (MDA) level of model group rats was significantly higher than that of blank group, while the MDA levels of the low-dose group and high-dose group were significantly lower than that of the model group. Forsythoside A is a potential drug substance for male infertility and improves the semen quality, MDA levels, and SOD activities of rats with oligoasthenospermia.

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Doroftei, Bogdan, Ovidiu-Dumitru Ilie, Maria Puiu, Alin Ciobica, and Ciprian Ilea. "Mini-Review Regarding the Applicability of Genome Editing Techniques Developed for Studying Infertility." Diagnostics 11, no. 2 (February 5, 2021): 246. http://dx.doi.org/10.3390/diagnostics11020246.

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Infertility is a highly debated topic today. It has been long hypothesized that infertility has an idiopathic cause, but recent studies demonstrated the existence of a genetic substrate. Fortunately, the methods of editing the human genome proven to be revolutionary. Following research conducted, we identified a total of 21 relevant studies; 14 were performed on mice, 5 on zebrafish and 2 on rats. We concluded that over forty-four genes in total are dispensable for fertility in both sexes without affecting host homeostasis. However, there are genes whose loss-of-function induces moderate to severe phenotypic changes in both sexes. There were situations in which the authors reported infertility, exhibited by the experimental model, or other pathologies such as cryptorchidism, cataracts, or reduced motor activity. Overall, zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 are techniques that offer a wide range of possibilities for studying infertility, even to create mutant variants. It can be concluded that ZFNs, TALENs, and CRISPR/Cas9 are crucial tools in biomedical research.

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Kirakosyan, E. V., А. V. Lyundup, L. S. Aleksandrov, А. G. Demchenko, A. I. Ishchenko, A. P. Nikonov, M. I. Bokarev, et al. "Cell technologies and their potential for treatment of female infertility." Voprosy ginekologii, akušerstva i perinatologii 19, no. 4 (2020): 146–58. http://dx.doi.org/10.20953/1726-1678-2020-4-146-158.

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The review analyses the results of using mesenchymal stem cells (MSCs) to restore fertility. Research works have shown that MSCs have the ability to differentiate into empryonic cells under specific induction conditions, and also when transplanted to gonadal tissue. Most studies were performed on MSCs obtained from bone marrow and the umbilical cord. Promising approaches related to the use of cell therapy have shown their high efficacy in preclinical studies: the infertility models in rats, mice and rabbits resulted in pregnancy after cell therapy. Research in this field is intensive: by now there is information about 11 preclinical and 12 clinical studies. The first results of clinical studies are positive. Clinical studies have been planned and started to determine the effect of MSCs transplantation on management of infertility, and their results, among others, will be decisive for introduction of cell therapy of infertility into clinical practice. The findings are promising, but further clinical research is needed to assess the efficacy and safety of MSCs transplantation for fertility restoration. Key words: infertility, gynaecology, treatment, MSCs, stem cells

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Montenegro, Mary Lourdes, Camila M. Bonocher, Juliana Meola, Rafael L. Portella, Alfredo Ribeiro-Silva, Mariangela O. Brunaldi, Rui Alberto Ferriani, and Julio Cesar Rosa-e-Silva. "Effect of Physical Exercise on Endometriosis Experimentally Induced in Rats." Reproductive Sciences 26, no. 6 (September 19, 2018): 785–93. http://dx.doi.org/10.1177/1933719118799205.

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Objective: Endometriosis is characterized by the growth of endometrial tissue outside the uterine cavity. The prevalence of endometriosis among women experiencing pain, infertility, or both is as high as 35% to 50%. The most common symptoms of endometriosis are dysmenorrhea, dyspareunia, chronic pelvic pain, and infertility. Evidence has suggested that endometriosis symptoms result from a local inflammatory peritoneal reaction caused by ectopic endometrial implants that undergo cyclic bleeding. On the other hand, regular physical exercise seems to have protective effects against diseases that involve inflammatory processes such as type 2 diabetes and colon and breast cancer. On this basis, it is possible that the practice of physical exercise may have beneficial effects on endometriosis. Therefore, the objective of this study was to evaluate the possible anti-inflammatory effect of physical exercise on endometriosis experimentally induced in rats. Study Design: Seventy female Wistar rats were divided into 7groups of 10 animals each. Animals performed light exercise (swimming once a week), moderate exercise (swimming 3 times a week), and intense exercise (swimming 5 times a week) before or after endometriosis induction. Results: At the end of the experimental protocol, a reduction in the size of endometriotic lesions was observed after physical exercise regardless of its frequency, with a greater reduction in the groups practicing moderate and intense activity; an increase in FAS levels and a decrease in matrix metalloproteinases 9 and proliferating cell nuclear antigen (PCNA)levels was also observed. The immunohistochemistry results did not lead to conclusive results. As expected, oxidative stress was reduced in all groups. These results show that the practice of physical exercise could be beneficial, at least in part, for the treatment of endometriosis.

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Krishnamoorthy, Gunasekaran, Kandaswamy Selvakumar, Prabhu Venkataraman, Perumal Elumalai, and Jagadeesan Arunakaran. "Lycopene supplementation prevents reactive oxygen species mediated apoptosis in Sertoli cells of adult albino rats exposed to polychlorinated biphenyls." Interdisciplinary Toxicology 6, no. 2 (June 1, 2013): 83–92. http://dx.doi.org/10.2478/intox-2013-0015.

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Abstract Sertoli cell proliferation is attenuated before attaining puberty and the number is fixed in adult testes. Sertoli cells determine both testis size and daily sperm production by providing physical and metabolic support to spermatogenic cells. Polychlorinated biphenyls (PCBs) exposure disrupts functions of Sertoli cells causing infertility with decreased sperm count. On the other hand, lycopene is improving sperm count and motility by reducing oxidative stress in humans and animals. Hence we hypothesized that PCBs-induced infertility might be due to Sertoli cell apoptosis mediated by oxidative stress and lycopene might prevent PCBs-induced apoptosis by acting against oxidative stress. To test this hypothesis, animals were treated with vehicle control, lycopene, PCBs and PCBs + lycopene for 30 days. After the experimental period, the testes and cauda epididymidis were removed for isolation of Sertoli cells and sperm, respectively. We observed increased levels of oxidative stress markers (H2O2 and LPO) levels, increased expression of apoptotic molecules (caspase-8, Bad, Bid, Bax, cytochrome C and caspase-3), decreased anti-apoptotic (Bcl2) molecule and elevated apoptotic marker activity (caspase-3) in Sertoli cells of PCBs-exposed animals. These results were associated with decreased sperm count and motility in PCBs exposed animals. On the other hand, lycopene prevented the elevation of Sertoli cellular apoptotic parameters and prevented the reduction of sperm parameters (count and motility). The data confirmed that lycopene as an antioxidant scavenged reactive oxygen substances, prevented apoptosis, maintained normal function in Sertoli cells and helped to provide physical and metabolic support for sperm production, thereby treating infertility in men.

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Demyashkin, Grigory A., Aleksandr Yu Tsibulevsky, Andrey A. Nedorubov, Yuliya V. Ivchenko, and Vladimir I. Shchekin. "THE MORPHOLOGICAL CHARACTERISTICS OF THE OFFSPRING OF MALE RATS IRRADIATED WITH ELECTRONS AT A DOSE OF 2 GY AND TREATMENT BY PLATELET-RICH PLASMA." Morphological newsletter 30, no. 1 (February 13, 2022): 49–55. http://dx.doi.org/10.20340/mv-mn.2022.30(1).614.

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Abstract:

One of the most dangerous effects of radiation on health is reproductive dysfunction, often leading to infertility. Available studies on the effects of radiation on spermatogenesis and fertility have not considered the possibility of using restorative therapy. One of the modern methods of infertility treatment is the introduction of autologous blood plasma with an increased content of platelets and native growth factors. Currently, there are no studies on the study of offspring obtained by using this method of infertility treatment and the assessment of its teratogenicity. Purpose of the study: morphological analysis of offspring of male rats irradiated with electrons at a dose of 2 Gy and receiving platelet-rich plasma. Male Wistar rats (n=35) were once subjected to targeted ionizing radiation at a NOVAC-11 pulsed electron accelerator at a dose of 2 Gy. To half (n=15) of the irradiated animals were injected with platelet-rich plasma and insulin-like growth factor-1 for 12 weeks, after which the males of both groups were placed with intact females (n=30) to produce offspring. The state of the developing offspring was assessed by morphological analysis after the removal of pregnant females from the experiment on the 20th day of prenatal development. Pregnancy was observed only in females bred by control males and males treated with platelet-rich plasma. Classical histological methods and X-ray computed microtomography were used. As a result of the study, it was found that the weight and size of the offspring from the control and experimental groups did not have macroscopic differences; light microscopy did not reveal significant changes in their histological structure. Pathological changes in the development of the offspring of males of the experimental group who received platelet-rich plasma after electron irradiation with a dose of 2 Gy using computed microtomography were also not detected. The obtained data indicate about of effectiveness of the treatment of the infertility of irradiated male rats with platelet-rich plasma, as well as the absence of its teratogenic effects.

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Journal articles on the topic 'Rats Infertility'

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