Academic literature on the topic 'Rats – Digestive organs – Histochemistry'

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Journal articles on the topic "Rats – Digestive organs – Histochemistry"

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Pekkarinen, M. "Scanning electron microscopy, whole-mount histology, and histochemistry of two anodontine glochidia (Bivalvia: Unionidae)." Canadian Journal of Zoology 74, no. 11 (November 1, 1996): 1964–73. http://dx.doi.org/10.1139/z96-223.

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Intramarsupial glochidia of Anodonta anatina (L.) and Pseudanodonta complanata (Rossmässler) were studied in southern Finland. Material staining positively with periodic acid – Schiff's reagent (PAS), neutral lipid reserves, and acid phosphatase activity have different distributions in the mantle of the two species. Moreover, the mucous covering of the mantle of the two glochidia behaves differently on critical-point drying. The presence of microvilli with alkaline phosphatase activity on the mantle surface and acid phosphatase activity in the mantle cells in both glochidia suggest that the mantle plays a role in nutrient uptake and digestion and possibly also in electrolyte uptake. The primordia of the stomach, digestive diverticula, and intestine, at least in A. anatina glochidia, contain neutral lipids and exhibit acid phosphatase activity: In A. anatina glochidia, a microvillous layer with alkaline phosphatase activity continues from the ventral walls of the lateral pits to the suspected kidney diverticula. In both glochidia, there may be three pairs of rudimentary ganglia, which do not stain with methylene blue. The eight ciliated sense organs of the glochidia are methylene blue- and PAS-positive and they exhibit succinate dehydrogenase and acid phosphatase activity. In each mantle lobe, the enveloping cell of the dorsal ciliary organ is interconnected with those of the ventral triad via a cellular fold or "tract," and the ciliated central cells of the organs send axons towards each other.
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Tsibulevskii, A. Yu, M. D. Polivoda, and A. P. �ttinger. "Pharmacological correction of postvagotomy hypoxia of the digestive organs by dibunol in rats." Bulletin of Experimental Biology and Medicine 111, no. 6 (June 1991): 834–36. http://dx.doi.org/10.1007/bf00840197.

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Ikegami, Sachie, Fumie Tsuchihashi, Hironobu Harada, Noboru Tsuchihashi, Eiichi Nishide, and Satoshi Innami. "Effect of Viscous Indigestible Polysaccharides on Pancreatic-Biliary Secretion and Digestive Organs in Rats." Journal of Nutrition 120, no. 4 (April 1, 1990): 353–60. http://dx.doi.org/10.1093/jn/120.4.353.

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Delp, M. D., R. O. Manning, J. V. Bruckner, and R. B. Armstrong. "Distribution of cardiac output during diurnal changes of activity in rats." American Journal of Physiology-Heart and Circulatory Physiology 261, no. 5 (November 1, 1991): H1487—H1493. http://dx.doi.org/10.1152/ajpheart.1991.261.5.h1487.

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Rat locomotor and feeding behavior varies on a diurnal basis; at night the animals actively forage and eat, whereas during the day they are more inactive and somnolent. At night, cardiac output is higher, presumably for enhanced perfusion of the active muscles to support increased metabolism and for enhanced perfusion of the digestive organs to support increased digestion and nutrient absorption. Conversely, it is hypothesized that during the daytime, blood flow to these two tissues is relatively low. The purpose of this study was to test these hypotheses by measuring cardiac output and the distribution of cardiac output in rats at various times in the diurnal cycle (8:00 A.M., 4:00 P.M., and 8:00 P.M.). The radiolabeled microsphere technique was used to measure cardiac output and distribution of blood flow to the tissues. Distribution of the total cardiac output was accounted for by complete dissection, weighing, and counting of organs and carcass. Cardiac output at 8:00 P.M. (136 +/- 9 ml/min) was elevated 13% (P less than 0.05) over that at 4:00 P.M. The proportion of the cardiac output distributed to the skeletal muscles (4:00 P.M.: 25%; 8:00 P.M.: 27%) and to the digestive tract (4:00 P.M.: 14%; 8:00 P.M.: 14%) did not change between the two time periods. Thus total muscle blood flow increased (P less than 0.05) from 31 +/- 2 at 4:00 P.M. to 36 +/- 4 ml/min at 8:00 P.M.; the only digestive organ to show a significant increase in blood flow from 4:00 P.M. to 8:00 P.M. was the stomach (133 +/- 17 to 166 +/- 19 ml/min, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
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Putri, Hemasayu Nirmala, Budiarto Budiarto, Arimbi Arimbi, Lucia Tri Suwanti, Kusnoto Kusnoto, and Soeharsono Soeharsono. "Heminthiasis in a Wild Rats (Rattus sp.) in Surabaya." Journal of Parasite Science 3, no. 2 (September 12, 2019): 73. http://dx.doi.org/10.20473/jops.v3i2.16521.

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The research was done to investigated and find out whether in the rat’s (Rattus sp.) body in Surabaya contained zoonotic helminth.This research used 31 rats taken from North Surabaya, South Surabaya, East Surabaya, West Surabaya and Central Surabaya, regardless of gender and age. 1 sample from Mulyosari, 3 samples from Bratang, 7 samples from Pegirian, and 20 samples came from Keputran. The result of 31 samples of rat’s digestive organs examine using a surgical method showed positive resulth of the presence of Hymenolepis diminutain the intestine and 5 positive samples were Capillaria hepatica, and then 25 positive samples were Taenia taeniaformis obtained from rat liver organs. From exploration using a microscope, 27 samples showed positive result in the presence of Capillaria hepatica’s eggs. Helminth found in this research are Hymenolepis diminuta, Taenia taeniaformis. and Capillaria hepatica which are zoonotic.
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Egorova, V. V., A. A. Nikitina, and N. M. Timofeeva. "Functioning of Enzyme Systems of Digestive and Non-Digestive Organs in Adult Rats Depends on Quality of Nutrition in Early Ontogenesis." Journal of Evolutionary Biochemistry and Physiology 41, no. 6 (November 2005): 640–46. http://dx.doi.org/10.1007/s10893-006-0005-2.

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Vovkun, Tetyana V., Petro I. Yanchuk, Lydia Y. Shtanova, and Anatoliy S. Shalamay. "Tissue Blood Flow in the Digestive Organs of Rats with Acute Pancreatitis after Corvitin Administration." International Journal of Physiology and Pathophysiology 8, no. 1 (2017): 25–32. http://dx.doi.org/10.1615/intjphyspathophys.v8.i1.30.

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Vovkun, T. V., P. I. Yanchuk, L. Y. Shtanova, and A. S. Shalamaу. "TISSUE BLOOD FLOW IN THE DIGESTIVE ORGANS OF RATS WITH ACUTE PANCREATITIS AFTER CORVITIN ADMINISTRATION." Fiziolohichnyĭ zhurnal 61, no. 6 (October 30, 2015): 53–59. http://dx.doi.org/10.15407/fz61.06.053.

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Puccio, F., and T. Lehy. "Bombesin ingestion stimulates epithelial digestive cell proliferation in suckling rats." American Journal of Physiology-Gastrointestinal and Liver Physiology 256, no. 2 (February 1, 1989): G328—G334. http://dx.doi.org/10.1152/ajpgi.1989.256.2.g328.

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The characterization of a bombesin-like peptide in the breast milk of some mammals might suggest that this peptide may influence, in part, the postnatal development of the digestive tract. To test this hypothesis, our experiments investigated whether oral administration of bombesin affects epithelial progenitor cell proliferation in digestive organs of suckling and weaned rats. Four series of pups were given bombesin diluted in milk (20 micrograms/kg, 3 times daily) or milk alone, for 5 days during either the first, second, third, or fourth week of life. Pups were killed after [3H]thymidine pulse labeling. DNA labeling and mitotic indices were estimated in the oxyntic, antral, colonic mucosae, and exocrine pancreas. In all tissues examined, oral bombesin significantly increased over control values the two cell proliferative parameters studied during the suckling period (P less than 0.05 to P less than 0.001). In bombesin-treated rats, maximal stimulation of these parameters occurred in the second week of life. This effect of oral bombesin on the cell kinetics disappeared in all tissues after weaning. This study confirms the growth-promoting effect of bombesin observed on the digestive system of the neonatal rat after subcutaneous administration of the peptide and shows that bombesin, when given orally to suckling rats, is sufficiently resistant to proteolysis to enable it to exert a stimulatory effect on digestive cell proliferation. However, our findings as such do not prove that milk bombesin-like peptide has a physiological influence on the developing gastrointestinal tract and pancreas.
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Vinokur, Vladimir, Leonid Grinberg, Eduard Berenshtein, Menachem Gross, Jackob Moskovitz, Abraham Z. Reznick, Mordechai Chevion, and Ron Eliashar. "Methionine-centered redox cycle in organs of the aero-digestive tract of young and old rats." Biogerontology 10, no. 1 (June 26, 2008): 43–52. http://dx.doi.org/10.1007/s10522-008-9152-8.

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Dissertations / Theses on the topic "Rats – Digestive organs – Histochemistry"

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DeWitt, Jason A. "Intracellular levels of reduced and oxidized glutathione in the tissue zones of the rat kidney." Virtual Press, 1999. http://liblink.bsu.edu/uhtbin/catkey/1137834.

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The purpose of this study was to determine the levels of whole tissue, cytosolic, and mitochondrial glutathione in the tissue zones of the rat kidney. Reduced (GSH) and oxidized glutathione (GSSG) were measured spectrophotometrically in tissue homogenates. Differential centrifugation was used to isolate the cytosolic and mitochondrial fractions. Cortical GSH and GSSG levels accounted for 51% and 60%, respectively, of the GSH and GSSG levels in the whole kidney. Cytosolic GSH levels were similar in the cortex and medulla but lower in the papilla. Cytosolic GSSG levels were highest in the cortex and lowest in the medulla. Mitochondrial GSH and GSSG levels did not follow a pattern similar to that of the cytosol or whole tissue. The mitochondrial redox ratio (GSH/GSH + GSSG X 100) was significantly higher in the cortex (ie., 67%) than the medulla (ie., 39%). The cytosolic redox ratio showed an opposite relationship with the cortex (ie., 57%) being lower than the medulla (ie., 78%). This study demonstrates that there are differences in GSH levels, GSSG levels, and the redox ratio in the tissue zones of the rat kidney.
Department of Physiology and Health Science
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Davis, Laura D. R. "The Biodistribution of 14C in the Digestive Organs of Rats Fed [14C]CD14 Protein." Thesis, Université d'Ottawa / University of Ottawa, 2010. http://hdl.handle.net/10393/12911.

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Human milk contains ~ 25 µg/mL of soluble cluster of differentiation 14 (sCD14) protein, a pattern recognition receptor (PRR) that triggers the innate immune system to respond to bacterial lipopolysaccharide (LPS). To date, the role of CD14 in the digestive tract of breast fed infants has not been well characterized and is the subject of this thesis. To investigate the biodistribution of proteins such as CD14 in vivo, a novel method for 14C radiolabeling of proteins to high specific radioactivity was developed using in vacuo methylation. Bovine serum albumin (BSA) and casein were used as test proteins to determine the following: 1) The efficacy of the in vacuo radiolabeling procedure; 2) The extent of incorporation of the 14C-label into the organs of oro-gastric gavaged 10 day old Sprague Dawley rats. [14C]BSA, [14C]casein and [14C]CD14 were prepared with specific radioactivities of 10 400, 10 800 and 163 000 dpm/µg, respectively. After feeding 6.25 µg of 14C-labeled proteins, quantifiable levels of 14C were found in the stomach, jejunum, duodenum, ileum, large intestine, intestinal luminal flushes, blood, liver, spleen and kidneys of rats. The accumulation of radiolabel in the organs of [14C]CD14 fed rats was temporally and spatially distinct from [14C]BSA and [14C]casein. Most notably, the label persisted in the stomach 480 min post-gavage. To design a neonate animal model for biodistribution, the segmental and total gastrointestinal transit times (GItt) were measured in two litters of 10 and 15 day old Sprague Dawley rat pups using barium sulfate. Ten day old rat pups that remained with and without the dam had a total gastrointestinal transit time of 13.8 ± 0.9 hr and 9.3 ± 0.7 hr, respectively. This decrease (p<0.05) in total gastrointestinal transit time in the absence of the dam was age dependent, as it was not observed (p>0.05) in the 15 day old rat pup litter. The immunological impact of an exogenous sCD14 source was examined in human peripheral blood mononuclear cells (PBMC). Pre-treatment of CD14+ monocytes with sCD14 had a protective effect, one of reducing the production of proinflammatory cytokines (TNF-α, IL-6, IL-8, IL-1β) when challenged with LPS. 14C was absorbed by neonate rats upon ingestion of [14C]CD14 and exposure to relatively high concentrations of rCD14 led to a reduction in inflammation. This may be beneficial to initial gut colonization in breast-fed newborns.
Alexander Graham Bell NSERC CGS M scholarship. Japan Society for the Promotion of Sciences, Summer in Japan Fellowship. Funded by the Canadian Institutes of Health Research, Institute of Nutrition Metabolism and Diabetes Grant #82816 “Fate and function of breast milk and recombinant human CD14 at mammary and newborn gastrointestinal mucosal epithelia”.
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Steeb, Corinna-Britta. "Effects of insulin-like growth factor-I (IGF-I) peptides on the growth and function of the gastrointestinal tract in adult and sucking rats /." Title page, abstract and contents only, 1995. http://web4.library.adelaide.edu.au/theses/09PH/09phs813.pdf.

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Lemmey, Andrew Bruce. "Effects of insulin-like growth factors (IGFS) on recovery from gut resection in rats : a thesis submitted to the University of Adelaide, South Australia for the degree of Doctor of Philosophy." 1992, 1993. http://web4.library.adelaide.edu.au/theses/09PH/09phl554.pdf.

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Includes bibliographical references (leaves 159-213) Shows that IGF-I peptides are effective in diminishing post-surgical catabolism and enhancing adaptive gut hyperplasia in rats recovering from massive small bowel resection.
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Luick, Bret R. "Digesta passage rates in the rat." Thesis, 1990. http://hdl.handle.net/1957/27133.

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The movements of digestion markers in the gut of the rat were investigated and the results of the investigation are reported in two manuscripts. The objective of the first study was to determine the influence of particle size of dietary wheat bran, chosen as a representative insoluble fiber, on the mean retention time (MRT) of digesta in the rat. The study design included a control group to detect the influence of particle size of digestion marker on the observed retention times. Additionally, a comparison was made between bran and the soluble fiber pectin. Both the soluble cobalt EDTA (Co EDTA) and insoluble chromium mordanted bran (CrMB), of two size ranges were administered to 4 groups of rats fed a semi-purified diet supplemented with wheat bran or pectin (10% dilution). Bran size and particulate marker size were large/large, large/small, small/small and pectin/small for the 4 groups. Mean retention times for CrMB were significantly longer in the pectin than the bran supplemented groups. Differences were not significantly different within the bran supplemented group. No significant differences were found between the rate of passage of CrMB and Co EDTA in any group. The second study was designed to identify pooling of markers in the rat gut. A mathematical model was developed as a system of equations which predicted the marker distribution along the gut following dosing. The model assumed that two pools, the stomach and cecum, were kinetically recognizable in the rat. The predicted distribution of CrMB and Co EDTA was compared to in vivo distribution at 4 h intervals by sequential termination and dissection of the animals. The smaller particle bran had a longer MRT in the cecum and a shorter MRT in the proximal colon. Total MRT to the mid-colon was similar in all bran supplemented groups. Pectin fed animals had significantly enlarged gut organs which explained the slower movement of marker in these animals. The cecum was found to delay the transit of marker from the small intestine to the proximal colon as much as 2.1 h. This delay resulted in the gut appearing as a single pool overall and therefore led the model to overpredict the initial rate of appearance of marker distal to the cecum. A streamlined system was developed to digest the fecal samples in preparation for atomic absorption spectroscopy, the method used to quantify digestion marker recovery. The development of the method is discussed and supporting data are presented on the reproducibility and limits of the method.
Graduation date: 1991
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Lemmey, Andrew Bruce. "Effects of insulin-like growth factors (IGFS) on recovery from gut resection in rats : a thesis submitted to the University of Adelaide, South Australia for the degree of Doctor of Philosophy / by Andrew Bruce Lemmey." 1992. http://hdl.handle.net/2440/21638.

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xxiii, 222 leaves : ill., plates ; 30 cm.
Title page, contents and abstract only. The complete thesis in print form is available from the University Library.
Shows that IGF-I peptides are effective in diminishing post-surgical catabolism and enhancing adaptive gut hyperplasia in rats recovering from massive small bowel resection.
Thesis (Ph.D.)--University of Adelaide, Dept. of Animal Science, 1992
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Books on the topic "Rats – Digestive organs – Histochemistry"

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T, Kapralova L., Termeleva A. G, and Orlov Viktor Nikolaevich, eds. Morfofunkt͡sionalʹnoe izuchenie organov pishchevarenii͡a kopytnykh: Metodicheskie rekomendat͡sii. Moskva: "Nauka", 1986.

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Luick, Bret R. Digesta passage rates in the rat. 1990.

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