Relevant bibliographies by topics / Rational Strain, Metabolic Engineering

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Rational Strain, Metabolic Engineering'

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Published: 6 September 2023

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Journal articles on the topic "Rational Strain, Metabolic Engineering"

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Tsouka, Sophia, Meric Ataman, Tuure Hameri, Ljubisa Miskovic, and Vassily Hatzimanikatis. "Constraint-based metabolic control analysis for rational strain engineering." Metabolic Engineering 66 (July 2021): 191–203. http://dx.doi.org/10.1016/j.ymben.2021.03.003.

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Freedman, Benjamin G., Parker W. Lee, and Ryan S. Senger. "Engineering the Metabolic Profile of Clostridium cellulolyticum with Genomic DNA Libraries." Fermentation 9, no. 7 (June 27, 2023): 605. http://dx.doi.org/10.3390/fermentation9070605.

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Clostridium cellulolyticum H10 (ATCC 35319) has the ability to ferment cellulosic substrates into ethanol and weak acids. The growth and alcohol production rates of the wild-type organism are low and, therefore, targets of metabolic engineering. A genomic DNA expression library was produced by a novel application of degenerate oligonucleotide primed PCR (DOP-PCR) and was serially enriched in C. cellulolyticum grown on cellobiose in effort to produce fast-growing and productive strains. The DNA library produced from DOP-PCR contained gene-sized DNA fragments from the C. cellulolyticum genome and from the metagenome of a stream bank soil sample. The resulting enrichment yielded a conserved phage structural protein fragment (part of Ccel_2823) from the C. cellulolyticum genome that, when overexpressed alone, enabled the organism to increase the ethanol yield by 250% compared to the plasmid control strain. The engineered strain showed a reduced production of lactate and a 250% increased yield of secreted pyruvate. Significant changes in growth rate were not seen in this engineered strain, and it is possible that the enriched protein fragment may be combined with the existing rational metabolic engineering strategies to yield further high-performing cellulolytic strains.
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Burgardt, Arthur, Ludovic Pelosi, Mahmoud Hajj Chehade, Volker F. Wendisch, and Fabien Pierrel. "Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production." Metabolites 12, no. 5 (May 11, 2022): 428. http://dx.doi.org/10.3390/metabo12050428.

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Coenzyme Q10 (CoQ10) is a lipid-soluble compound with important physiological functions and is sought after in the food and cosmetic industries owing to its antioxidant properties. In our previous proof of concept, we engineered for CoQ10 biosynthesis the industrially relevant Corynebacterium glutamicum, which does not naturally synthesize any CoQ. Here, liquid chromatography–mass spectrometry (LC–MS) analysis identified two metabolic bottlenecks in the CoQ10 production, i.e., low conversion of the intermediate 10-prenylphenol (10P-Ph) to CoQ10 and the accumulation of isoprenologs with prenyl chain lengths of not only 10, but also 8 to 11 isopentenyl units. To overcome these limitations, the strain was engineered for expression of the Ubi complex accessory factors UbiJ and UbiK from Escherichia coli to increase flux towards CoQ10, and by replacement of the native polyprenyl diphosphate synthase IspB with a decaprenyl diphosphate synthase (DdsA) to select for prenyl chains with 10 isopentenyl units. The best strain UBI6-Rs showed a seven-fold increased CoQ10 content and eight-fold increased CoQ10 titer compared to the initial strain UBI4-Pd, while the abundance of CoQ8, CoQ9, and CoQ11 was significantly reduced. This study demonstrates the application of the recent insight into CoQ biosynthesis to improve metabolic engineering of a heterologous CoQ10 production strain.
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Zhu, Linghuan, Sha Xu, Youran Li, and Guiyang Shi. "Improvement of 2-phenylethanol production in Saccharomyces cerevisiae by evolutionary and rational metabolic engineering." PLOS ONE 16, no. 10 (October 19, 2021): e0258180. http://dx.doi.org/10.1371/journal.pone.0258180.

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2-Phenylethanol (2-PE) is a valuable aromatic compound with favorable flavors and good properties, resulting in its widespread application in the cosmetic, food and medical industries. In this study, a mutant strain, AD032, was first obtained by adaptive evolution under 2-PE stress. Then, a fusion protein from the Ehrlich pathway, composed of tyrB from Escherichia coli, kdcA from Lactococcus lactis and ADH2 from Saccharomyces cerevisiae, was constructed and expressed. As a result, 3.14 g/L 2-PE was achieved using L-phenylalanine as a precursor. To further increase 2-PE production, L-glutamate oxidase from Streptomyces overexpression was applied for the first time in our research to improve the supply of α-ketoglutarate in the transamination of 2-PE synthesis. Furthermore, we found that the disruption of the pyruvate decarboxylase encoding gene PDC5 caused an increase in 2-PE production, which has not yet been reported. Finally, assembly of the efficient metabolic modules and process optimization resulted in the strain RM27, which reached 4.02 g/L 2-PE production from 6.7 g/L L-phenylalanine without in situ product recovery. The strain RM27 produced 2-PE (0.8 mol/mol) with L-phenylalanine as a precursor, which was considerably high, and displayed manufacturing potential regarding food safety and process simplification aspects. This study suggests that innovative strategies regarding metabolic modularization provide improved prospects for 2-PE production in food exploitation.
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Nevoigt, Elke. "Progress in Metabolic Engineering of Saccharomyces cerevisiae." Microbiology and Molecular Biology Reviews 72, no. 3 (September 2008): 379–412. http://dx.doi.org/10.1128/mmbr.00025-07.

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SUMMARY The traditional use of the yeast Saccharomyces cerevisiae in alcoholic fermentation has, over time, resulted in substantial accumulated knowledge concerning genetics, physiology, and biochemistry as well as genetic engineering and fermentation technologies. S. cerevisiae has become a platform organism for developing metabolic engineering strategies, methods, and tools. The current review discusses the relevance of several engineering strategies, such as rational and inverse metabolic engineering, evolutionary engineering, and global transcription machinery engineering, in yeast strain improvement. It also summarizes existing tools for fine-tuning and regulating enzyme activities and thus metabolic pathways. Recent examples of yeast metabolic engineering for food, beverage, and industrial biotechnology (bioethanol and bulk and fine chemicals) follow. S. cerevisiae currently enjoys increasing popularity as a production organism in industrial (“white”) biotechnology due to its inherent tolerance of low pH values and high ethanol and inhibitor concentrations and its ability to grow anaerobically. Attention is paid to utilizing lignocellulosic biomass as a potential substrate.
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Natarajan, Aravind, Thapakorn Jaroentomeechai, Mingji Li, Cameron J. Glasscock, and Matthew P. DeLisa. "Metabolic engineering of glycoprotein biosynthesis in bacteria." Emerging Topics in Life Sciences 2, no. 3 (August 30, 2018): 419–32. http://dx.doi.org/10.1042/etls20180004.

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The demonstration more than a decade ago that glycoproteins could be produced in Escherichia coli cells equipped with the N-linked protein glycosylation machinery from Campylobacter jejuni opened the door to using simple bacteria for the expression and engineering of complex glycoproteins. Since that time, metabolic engineering has played an increasingly important role in developing and optimizing microbial cell glyco-factories for the production of diverse glycoproteins and other glycoconjugates. It is becoming clear that future progress in creating efficient glycoprotein expression platforms in bacteria will depend on the adoption of advanced strain engineering strategies such as rational design and assembly of orthogonal glycosylation pathways, genome-wide identification of metabolic engineering targets, and evolutionary engineering of pathway performance. Here, we highlight recent advances in the deployment of metabolic engineering tools and strategies to develop microbial cell glyco-factories for the production of high-value glycoprotein targets with applications in research and medicine.
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Tafur Rangel, Albert E., Abel García Oviedo, Freddy Cabrera Mojica, Jorge M. Gómez, and Andrés Fernando Gónzalez Barrios. "Development of an integrating systems metabolic engineering and bioprocess modeling approach for rational strain improvement." Biochemical Engineering Journal 178 (January 2022): 108268. http://dx.doi.org/10.1016/j.bej.2021.108268.

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Zhang, Xiaomei, Zhenhang Sun, Jinyu Bian, Yujie Gao, Dong Zhang, Guoqiang Xu, Xiaojuan Zhang, Hui Li, Jinsong Shi, and Zhenghong Xu. "Rational Metabolic Engineering Combined with Biosensor-Mediated Adaptive Laboratory Evolution for l-Cysteine Overproduction from Glycerol in Escherichia coli." Fermentation 8, no. 7 (June 25, 2022): 299. http://dx.doi.org/10.3390/fermentation8070299.

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l-Cysteine is an important sulfur-containing amino acid with numerous applications in the pharmaceutical and cosmetic industries. The microbial production of l-cysteine has received substantial attention, and the supply of the precursor l-serine is important in l-cysteine biosynthesis. In this study, to achieve l-cysteine overproduction, we first increased l-serine production by deleting genes involved in the pathway of l-serine degradation to glycine (serine hydroxymethyl transferase, SHMT, encoded by glyA genes) in strain 4W (with l-serine titer of 1.1 g/L), thus resulting in strain 4WG with l-serine titer of 2.01 g/L. Second, the serine-biosensor based on the transcriptional regulator NCgl0581 of C. glutamicum was constructed in E. coli, and the validity and sensitivity of the biosensor were demonstrated in E. coli. Then 4WG was further evolved through adaptive laboratory evolution (ALE) combined with serine-biosensor, thus yielding the strain 4WGX with 4.13 g/L l-serine production. Moreover, the whole genome of the evolved strain 4WGX was sequenced, and ten non-synonymous mutations were found in the genome of strain 4WGX compared with strain 4W. Finally, 4WGX was used as the starting strain, and deletion of the l-cysteine desulfhydrases (encoded by tnaA), overexpression of serine acetyltransferase (encoded by cysE) and the key enzyme of transport pathway (encoded by ydeD) were performed in strain 4WGX. The recombinant strain 4WGX-∆tnaA-cysE-ydeD can produce 313.4 mg/L of l-cysteine using glycerol as the carbon source. This work provides an efficient method for the biosynthesis of value-added commodity products associated with glycerol conversion.
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Iacometti, Camillo, Katharina Marx, Maria Hönick, Viktoria Biletskaia, Helena Schulz-Mirbach, Beau Dronsella, Ari Satanowski, et al. "Activating Silent Glycolysis Bypasses in Escherichia coli." BioDesign Research 2022 (May 12, 2022): 1–17. http://dx.doi.org/10.34133/2022/9859643.

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All living organisms share similar reactions within their central metabolism to provide precursors for all essential building blocks and reducing power. To identify whether alternative metabolic routes of glycolysis can operate in E. coli, we complementarily employed in silico design, rational engineering, and adaptive laboratory evolution. First, we used a genome-scale model and identified two potential pathways within the metabolic network of this organism replacing canonical Embden-Meyerhof-Parnas (EMP) glycolysis to convert phosphosugars into organic acids. One of these glycolytic routes proceeds via methylglyoxal and the other via serine biosynthesis and degradation. Then, we implemented both pathways in E. coli strains harboring defective EMP glycolysis. Surprisingly, the pathway via methylglyoxal seemed to immediately operate in a triosephosphate isomerase deletion strain cultivated on glycerol. By contrast, in a phosphoglycerate kinase deletion strain, the overexpression of methylglyoxal synthase was necessary to restore growth of the strain. Furthermore, we engineered the “serine shunt” which converts 3-phosphoglycerate via serine biosynthesis and degradation to pyruvate, bypassing an enolase deletion. Finally, to explore which of these alternatives would emerge by natural selection, we performed an adaptive laboratory evolution study using an enolase deletion strain. Our experiments suggest that the evolved mutants use the serine shunt. Our study reveals the flexible repurposing of metabolic pathways to create new metabolite links and rewire central metabolism.
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Jeong, Sun-Wook, Jun-Ho Kim, Ji-Woong Kim, Chae Yeon Kim, Su Young Kim, and Yong Jun Choi. "Metabolic Engineering of Extremophilic Bacterium Deinococcus radiodurans for the Production of the Novel Carotenoid Deinoxanthin." Microorganisms 9, no. 1 (December 25, 2020): 44. http://dx.doi.org/10.3390/microorganisms9010044.

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Deinoxanthin, a xanthophyll derived from Deinococcus species, is a unique organic compound that provides greater antioxidant effects compared to other carotenoids due to its superior scavenging activity against singlet oxygen and hydrogen peroxide. Therefore, it has attracted significant attention as a next-generation organic compound that has great potential as a natural ingredient in a food supplements. Although the microbial identification of deinoxanthin has been identified, mass production has not yet been achieved. Here, we report, for the first time, the development of an engineered extremophilic microorganism, Deinococcus radiodurans strain R1, that is capable of producing deinoxanthin through rational metabolic engineering and process optimization. The genes crtB and dxs were first introduced into the genome to reinforce the metabolic flux towards deinoxanthin. The optimal temperature was then identified through a comparative analysis of the mRNA expression of the two genes, while the carbon source was further optimized to increase deinoxanthin production. The final engineered D. radiodurans strain R1 was able to produce 394 ± 17.6 mg/L (102 ± 11.1 mg/g DCW) of deinoxanthin with a yield of 40.4 ± 1.2 mg/g sucrose and a productivity of 8.4 ± 0.2 mg/L/h from 10 g/L of sucrose. The final engineered strain and the strategies developed in the present study can act as the foundation for the industrial application of extremophilic microorganisms.
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Dissertations / Theses on the topic "Rational Strain, Metabolic Engineering"

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Carere, Robert Carlo. "Genomics of cellulolytic clostridia and development of rational metabolic engineering strategies." MPI Open Access Journals, 2008. http://hdl.handle.net/1993/21707.

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Consolidated bioprocessing, a process in which cellulase production, substrate hydrolysis, and fermentation occur simultaneously, offers the potential for lower biofuel production costs than traditional approaches and is an economically attractive near-term goal for fermentative production of ethanol and/or hydrogen (H2) as biofuels. Current yields fall short of theoretical maxima, vary considerably between species, and are influenced by the highly branched metabolic pathways utilized by fermentative organisms. For fermentative ethanol/ H2 production to become practical, yields must be increased either through intelligent species selection, a manipulation of culture conditions, or via the implementation of rational metabolic engineering strategies. A comparative genomics approach amoungst select members of the Firmicutes, Euryarchaeota, and Thermotogae was used to identify genes relevent to ethanol and H2 production. Growth, end-product synthesis, enzyme activities and the associated transcription of select genes were studied in the cellulolytic anaerobe, Clostridium thermocellum ATCC 27405, during batch fermentation of cellobiose to determine the effect of elevated N2 and H2 sparging on end-product distribution. The absence of genes encoding acetaldehyde dehydrogenase and bifunctional acetaldehyde/alcohol dehydrogenase (AdhE) correlates with elevated H2 yields and low ethanol production. The type(s) of encoded hydrogenases appear to have minimal impact on H2 production in organisms that do not encode ethanologenic pathways, however, they do influence reduced end-product yields in those that do. We also find that while gas sparging can be used to effectively shift carbon and electron flow, the observed shifts at the pyruvate branch-point are likely principally influenced by the availability of reduced electron carriers (NAD, NADP, ferredoxin) and thermodynamic considerations. Finally, both electrotransformation and conjugative plasmid protocols were developed and evaluated for thermophilic species C. thermocellum and Thermoanaerobacter pseudethanolicus 39E, and the mesophilic bacterium, Clostridium termitidis CT1112. The efficiency of transformation for C. thermocellum strain ATCC 27405 is consistently low whereas transformation frequencies were ~100-fold higher in C. termitidis. Observed frequencies of plasmid transfer, via conjugation, were similar in both C. thermocellum and C. termitidis suggesting the transfer of single stranded DNA may circumvent aggressive restriction methylation systems.
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Chen, Lin [Verfasser]. "Rational Metabolic Engineering and Systematic Analysis of Escherichia coli for L-Tryptophan Bioproduction / Lin Chen." München : Verlag Dr. Hut, 2017. http://d-nb.info/1128466961/34.

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Schiefelbein, Sarah [Verfasser], and Christoph [Akademischer Betreuer] Wittmann. "Improved L-lysine production in Corynebacterium glutamicum by rational strain engineering / Sarah Schiefelbein ; Betreuer: Christoph Wittmann." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2015. http://d-nb.info/112757969X/34.

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Hills, Christopher. "Acetate metabolism in Geobacillus thermoglucosidasius and strain engineering for enhanced bioethanol production." Thesis, University of Bath, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.665397.

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Social, economic and political pressures have driven the development of renewable alternatives to fossil fuels. Biofuels, such as bioethanol, have proved to be successful alternatives. Mature technologies are crop-based, but this has brought criticism due to the conflicting use of land for fuel versus food production. Therefore, bioethanol production technologies have shifted to utilising the sugars that derive from the degradation of lignocellulosic biomass. The thermophilic, Gram-positive bacterium, Geobacillus thermoglucosidasius, can naturally utilise a large fraction of these sugars, and metabolic engineering has been used to create a strain that produces ethanol as the major product of fermentation. This strain, G. thermoglucosidasius TM242 (Δldh, Δpfl, pdhup), does however, produce small but significant quantities of acetate, an undesirable by-product of fermentation. Therefore, acetate metabolism in the G. thermoglucosidasius TM242 strain was the focus of this study. During fermentation, ethanol is generated from the central metabolite acetyl-CoA through the activities of a bifunctional enzyme: aldehyde dehydrogenase/alcohol dehydrogenase (ADHE). On the other hand, acetate is generated from acetyl-CoA through catalysis by phosphotransacetylase (PTA) and acetate kinase (AK). Acetate metabolism in G. thermoglucosidasius TM242 was studied in this project by investigating the enzyme activities governing flux from acetyl-CoA, and the feasibility of reduced acetate production was investigated by a pta-deletion strategy. This thesis reports the characterisation of PTA and AK, by studying activities from both native cell lysates and recombinantly expressed proteins. The results indicate that the activities of PTA and AK are greater than those of ADHE, suggesting that the potential metabolic flux is greater towards acetate production than to ethanol. However, the ethanol yield from G. thermoglucosidasius TM242 fermentations is greater than that of acetate, suggesting the existence of a regulatory mechanism controlling acetyl-CoA flux. Several possible regulatory mechanisms were studied in this project and are reported here. The viability of creating a strain that reduces acetate accumulation, and potentially increases ethanol yields, was investigated and reported in this thesis. The gene encoding PTA was deleted from G. thermoglucosidasius TM242, and the resulting strain was characterised. The Δpta strain had approximately 5% of the PTA activity measured in TM242, but acetate was still generated from pentose and hexose fermentations. Additional phosphotransacylase (PTAC) enzymes were discovered in G. thermoglucosidasius TM242 that could catalyse the conversion of acetyl-CoA and orthophosphate to acetyl-phosphate and CoA. A series of PTAC null strains were created and analysed, the results of which indicated that phosphotransbutyrylase (PTB) could be involved in acetate production in vivo. It was discovered that the cell lysates of G. thermoglucosidasius strains carrying deletions to both pta and ptb could no longer catalyse the conversion of acetyl-CoA and orthophosphate to acetyl-phosphate and CoA. However, these strains still accumulated acetate, suggesting the presence of alternative acetate-producing pathways in this organism. In addition, G. thermoglucosidasius strains carrying deletions to both pta and ptb could ferment glucose but not xylose, suggesting that the production of ATP by the PTA-AK pathway is crucial for micro-aerobic growth on pentose sugars.
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Gerebring, Linnéa. "Yeast Saccharomyces cerevisiae strain isolated from lager beer shows tolerance to isobutanol." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-129066.

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The development of biofuels has received much attention due to the global warming and limited resources associated with fossil fuels. Butanol has been identified as a potential option due to its advantages over ethanol, for example higher energy density, compatibility with current infrastructure and its possibility to be blended with gasoline at any ratio. Yeast Saccharomyces cerevisiae can be used as a producer of butanol. However, butanol toxicity to the host limits the yield produced. In this study, four strains of yeast isolated from the habitats of lager beer, ale, wine and baker ́s yeast were grown in YPD media containing isobutanol concentrations of 1.5 %, 2 %, 3 % and 4 %. Growth was measured to determine the most tolerant strain. Gene expression for the genes RPN4, RTG1 and ILV2 was also measured, to determine its involvement in butanol stress. The genes have in previous studies seen to be involved in butanol tolerance or production, and the hypothesis was that they all should be upregulated in response to butanol exposure. It was found that the yeast strain isolated from lager beer was most tolerant to isobutanol concentrations of 2 % and 3 %. It was also found that the gene RPN4 was upregulated in response to isobutanol stress. There was no upregulation of RTG1 or ILV2, which was unexpected. The yeast strain isolated from lager beer and the gene RPN4 is proposed to be investigated further, to be able to engineer a suitable producer of the biofuel butanol.
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Bi, Changhao. "Metabolic characterization and engineering of Enterobacter asburiae strain JDR-1 to develop microbial biocatalysts for efficient hemicellulose utilization." [Gainesville, Fla.] : University of Florida, 2009. http://purl.fcla.edu/fcla/etd/UFE0024266.

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Kachel, Benjamin [Verfasser], and Michael [Akademischer Betreuer] Lanzer. "Metabolic engineering of Synechococcus sp. strain PCC 7002 for the photoautotrophic production of riboflavin (vitamin B2) / Benjamin Kachel ; Betreuer: Michael Lanzer." Heidelberg : Universitätsbibliothek Heidelberg, 2021. http://nbn-resolving.de/urn:nbn:de:bsz:16-heidok-305364.

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Freedman, Benjamin Gordon. "Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment." Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/71346.

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Combinatorial approaches in metabolic engineering can make use of randomized mutations and/or overexpression of randomized DNA fragments. When DNA fragments are obtained from a common genome or metagenome and packaged into the same expression vector, this is referred to as a DNA library. Generating quality DNA libraries that incorporate broad genetic diversity is challenging, despite the availability of published protocols. In response, a novel, efficient, and reproducible technique for creating DNA libraries was created in this research based on whole genome amplification using degenerate oligonucleotide primed PCR (DOP-PCR). The approach can produce DNA libraries from nanograms of a template genome or the metagenome of multiple microbial populations. The DOP-PCR primers contain random bases, and thermodynamics of hairpin formation was used to design primers capable of binding randomly to template DNA for amplification with minimal bias. Next-generation high-throughput sequencing was used to determine the design is capable of amplifying up to 98% of template genomic DNA and consistently out-performed other DOP-PCR primers. Application of these new DOP-PCR amplified DNA libraries was demonstrated in multiple strain enrichments to isolate genetic library fragments capable of (i) increasing tolerance of E. coli ER2256 to toxic levels of 1-butanol by doubling the growth rate of the culture, (ii) redirecting metabolism to ethanol and pyruvate production (over 250% increase in yield) in Clostridium cellulolyticum when consuming cellobiose, and (iii) enhancing L-arginine production when used in conjunction with a new synthetic gene circuit.
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Poblete, Castro Ignacio Andrés [Verfasser], and Christoph [Akademischer Betreuer] Wittmann. "Systems Biotechnology of Pseudomonas putida for the enhanced production of Polyhydroxyalkanoates: a rational approach for strain and bioprocess engineering / Ignacio Andrés Poblete Castro ; Betreuer: Christoph Wittmann." Braunschweig : Technische Universität Braunschweig, 2012. http://d-nb.info/1175823147/34.

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Montoya, Solano José David [Verfasser]. "Metabolic engineering of the Colombian strain Clostridium sp. IBUN 158B in order to improve the bioconversion of glycerol into 1,3-propanediol / José David Montoya Solano." Ulm : Universität Ulm. Fakultät für Naturwissenschaften, 2013. http://d-nb.info/1030045755/34.

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Books on the topic "Rational Strain, Metabolic Engineering"

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Voll, Lars M., and Zoran Nikoloski, eds. Engineering Synthetic Metabolons: From Metabolic Modelling to Rational Design of Biosynthetic Devices. Frontiers Media SA, 2016. http://dx.doi.org/10.3389/978-2-88919-921-1.

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Book chapters on the topic "Rational Strain, Metabolic Engineering"

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Ogbulie, Toochukwu Ekwutosi, Augusta Anuli Nwachukwu, Priscilla Amaka Ogbodo, and Christiana N. Opara. "Strategies for Yeast Strain Improvement through Metabolic Engineering." In Fermentation and Algal Biotechnologies for the Food, Beverage and Other Bioproduct Industries, 119–41. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003178378-7.

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Furusawa, Chikara, Takaaki Horinouchi, Takashi Hirasawa, and Hiroshi Shimizu. "Systems Metabolic Engineering: The Creation of Microbial Cell Factories by Rational Metabolic Design and Evolution." In Advances in Biochemical Engineering/Biotechnology, 1–23. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/10_2012_137.

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Roshchina, S., A. Gribanov, M. Lukin, D. Chibrikin, and Mei Shunqi. "Investigation of the Stress–Strain State of Wooden Beams with Rational Reinforcement with Composite Materials." In Lecture Notes in Civil Engineering, 475–83. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-85236-8_42.

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Evangelista, Pedro, Isabel Rocha, Eugénio C. Ferreira, and Miguel Rocha. "Evolutionary Approaches for Strain Optimization Using Dynamic Models under a Metabolic Engineering Perspective." In Evolutionary Computation, Machine Learning and Data Mining in Bioinformatics, 140–51. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-01184-9_13.

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Pal, Nirmalya, and Shikha Kapil Soni. "Development of cellulolytic thermotolerant fungal strain." In Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass, 137–42. Elsevier, 2020. http://dx.doi.org/10.1016/b978-0-12-817953-6.00009-9.

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Adebami, Gboyega E., and Bukola C. Adebayo-Tayo. "Development of cellulolytic strain by genetic engineering approach for enhanced cellulase production." In Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass, 103–36. Elsevier, 2020. http://dx.doi.org/10.1016/b978-0-12-817953-6.00008-7.

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Hivrale, Amol Uttam, Pankaj K. Pawar, Niraj R. Rane, and Sanjay P. Govindwar. "Application of Genomics and Proteomics in Bioremediation." In Advances in Environmental Engineering and Green Technologies, 97–112. IGI Global, 2016. http://dx.doi.org/10.4018/978-1-4666-9734-8.ch005.

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Bioremediation mediated by microorganisms is proving to be cost effective, ecofriendly and sustainable technology. Genome enable experimental and modeling techniques are of a great help in evaluating physiology and enhancing performance of life forms to be used for bioremediation purpose. Similarly, the application of proteomics in bioremediation research provides a global view of the protein composition of microbial cell and offers promising approach to understand the molecular mechanism of removal of toxic material from the environment. Combination of proteomics and genomics in bioremediation is an insight into global metabolic and regulatory network that can enhance the understanding of gene functions. Present chapter give a bird's eye view of genomics and proteomics and their potential utilization in bioremediation and for the clearer understanding of the cellular responses to environmental stimuli. An understanding of the growth conditions governing the expression of proteome in a specific environment is essential for developing rational strategies for successful bioremediation.
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Conference papers on the topic "Rational Strain, Metabolic Engineering"

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Hassani, Leila, Mohammad R. Moosavi, and Payam Setoodeh. "A Graph Based Approach to Analyse Metabolic Networks for Strain Engineering." In 2019 27th Iranian Conference on Electrical Engineering (ICEE). IEEE, 2019. http://dx.doi.org/10.1109/iraniancee.2019.8786434.

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Carino, Claudio, Fabio Carli, Carlo Cinquini, and Mauro Gobbi. "Pipe Self-Reinforcing Outlets: Nonlinear Analysis Towards Rational Design." In ASME 1995 Design Engineering Technical Conferences collocated with the ASME 1995 15th International Computers in Engineering Conference and the ASME 1995 9th Annual Engineering Database Symposium. American Society of Mechanical Engineers, 1995. http://dx.doi.org/10.1115/detc1995-0120.

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Abstract A class of self-reinforcing outlets addressed to a wide variety of plants and pipelines is analyzed in this paper in order to examine computer simulation in comparison with bursting tests commonly adopted in industry. Based on a specific pre-processor for mesh generation, finite element analysis is performed on a workstation by a software package suitably accounting for both geometrical and material nonlinearities. Besides stress distribution visualization, high-resolution post-processor capability allows to predict, with sufficient accuracy, plastic strain trend in critical area. This may provide, on the one hand, a powerful tool in view of anticipating proof testing results and, on the other hand, a useful guideline for design purposes in view of a more rational material usage and joint configuration, paying particular attention to the overall structural reliability and performance.
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Nguyen, Ngoc, Olav Fyrileiv, and Chor Yew Chia. "A Numerical Model for Submarine Pipelines With Concrete Coating." In ASME 2017 36th International Conference on Ocean, Offshore and Arctic Engineering. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/omae2017-62440.

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This paper presents a numerical model that is used to estimate the structural response of a submarine pipeline with concrete weight coating subjected to loadings commonly encountered in pipeline installation and operation phases. Findings from parametric studies performed with the numerical model are used to widen the applicable range of the simplified concrete crushing criterion in DNV-OS-F101 (2013) and formulate a rational approach for the design of pipeline concrete weight coating under typical installation and operation conditions. The rational design approach will allow for potential selection from a wider range of installation vessels and relaxation of the installation weather window criterion. The design method also provides insights into the strain concentration in the field joint at different strain levels, which is used to assess the field joint welding integrity for pipeline in free spans and in high strain conditions. The numerical model considers nonlinearities in steel and concrete material stress and strain, as well as complex adhesive behaviour of the anti-corrosion coating. Good agreement is obtained between the numerical results and existing experimental data for all the sections along the pipeline model where comparisons are made on moment–strain global behaviour, sliding from the concrete coating, hot spot strain near the field joint and concrete strain. The numerical program is performed within the scope of Phase 1 of the joint industry project called “Design of concrete coating for submarine pipelines”. Laboratory tests to check and improve the numerical model are planned for Phase 2.
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Nguyen, Ngoc, Olav Fyrileiv, and Chor Yew Chia. "Improving the Installation Criterion for Concrete Coated Pipelines." In ASME 2018 37th International Conference on Ocean, Offshore and Arctic Engineering. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/omae2018-78512.

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Current design practice limits the concrete strain to approximately 0.2% in a simplified design criterion. In most standard cases, this has proved to be safe and adequate. However, in recent years, the pipeline industry is extending into more remote, harsher environments and larger diameter pipelines. The use of the simplified design criterion has, in some circumstances, resulted in too strict installation requirements which limit the number of relevant installation vessels. This paper presents some findings on the concrete strain for submarine pipelines with concrete weight coating (CWC) derived from the numerical program performed within the scope of Phase 1 of the joint industry project “Design of concrete coating for submarine pipelines”. Non-linearities in the concrete weight coating, anti-corrosion coating (ACC) and steel material properties, as well as large deformation and the sequence of load application were included in the numerical model. The results from the numerical analyses have been well validated against existing experimental data, and the numerical model was subsequently used in an extensive parametric study, where the behaviour of concrete coated pipelines was investigated for monotonic and reversed bending with nominal strain up to 0.4%. These numerical results can be used to widen the applicable range of the simplified concrete crushing criterion in DNVGL-ST-F101 (2017), and to formulate a rational approach for the design of pipeline concrete weight coating under typical installation and operation conditions. The rational design approach will allow for a wider range of installation vessels to select from for installation of the pipeline, and relaxation of the installation weather window criterion.
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Yang, Zhengmao, Shashi Kumar, and Jens P. Tronskar. "ECA of Pipeline With Girth Weld Strength Mis-Matching Subjected to Large Strain." In ASME 2009 28th International Conference on Ocean, Offshore and Arctic Engineering. ASMEDC, 2009. http://dx.doi.org/10.1115/omae2009-79376.

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In recent years, the strain based design for pipeline has been widely accepted by the industry, but the definition of a rational flaw acceptance criteria for girth welds subjected to axial strain within the context of the existing codified fracture mechanics based assessment procedures is problematic since these are essentially stress based. To extend the FAD method to the large strain conditions, several challenges i.e. weld strength mismatching, fracture toughness, and welding residual stresses have to be understood. With appropriate modifications as per DNV-RP-F108 [1], the assessments procedure detailed in BS7910 document for stress based situations have been used successfully for several projects to develop acceptance criteria for pipeline installation involving plastic straining. But only weld metal strength over-match comparing with base metal is considered in DNV-RP-F108 [1]. High strength line pipes are required to reduce the transmission cost of natural gas in long distance and internal clad with corrosion resistant alloy (CRA) is used for transportation of sour gas. Steel manufactures have developed such line pipes to develop new oil and gas field. The inconel filler metal was selected as weld consumable for the production girth weld in the lay budge. From the all weld tensile tests, it was found that the yield strength of the weld is under-match comparing the base metal, and the pipeline maybe subjected to a strain level up to 1.0% due to the lateral buckling. In this research the effect of weld strength mismatching on the structural integrity of the pipeline subjected to large strain was studied. The Engineering Critical Assessment (ECA) was performed to derive the critical flaw acceptance criteria for the AUT system. The segment tests and numerical analysis were performed to validate the assessment procedure, and the finite element analyses of the pipeline girth weld with surface crack in the weld centre were carried out to investigate the effect of bi-axial loading on the ECA results.
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Conway, T. A., P. C. Lam, and N. Mazilu. "On a Theoretical Description of Hysteresis for Soft Tissues." In ASME 1996 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1996. http://dx.doi.org/10.1115/imece1996-1155.

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Abstract In complex loading conditions, it is important to know, on one hand, the uniaxial behavior of materials and, on the other hand, how this uniaxial behavior changes with the state of material. Soft tissues offer one of the most attractive areas to exercise a mathematical model for the cyclic loading. Particular classes of soft tissues are interesting in that they exhibit a homographic relation between stress and strain, during uniaxial quasistatic experiments [1]. Our investigation of soft tissues behavior shows that this is a consequence of the fact that we can adequately approximate the experimental curve by a rational function and consider the measured strain as the eigenvalue of a matrix [2]. Now, assuming that the homography is valid, as the experimental data seems to indicate, we may use it to develop a description of the strain hysteresis phenomenon. For most materials, a knowledge of the hysteresis per cycle helps to define its fatigue life in low or high cycle loading. In the case of living tissues, the analytical modeling of the hysteretic response is necessary to understand the contribution of the fatigue and wear components in tissue failure mechanisms.
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Cheng, Hsien-Chie, Kuo-Ning Chiang, and Chao-Kuang Chen. "Parametric Analysis of Thermally Enhanced BGA Reliability Using a Finite-Volume-Weighted Averaging Technique." In ASME 1998 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1998. http://dx.doi.org/10.1115/imece1998-0423.

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Abstract As the eager demand for high power-handling capability in smaller packages, thermally enhanced BGA (TEBGA) packages provide a very attractive solution in improving the poor thermal performance problems of conventional over molded plastic BGA packages. In this study, with solder joint reliability in concern during the initial package design stage, an engineering empirical approach using a finite-volume-weighted averaging technique is applied for characterizing the strain concentration field around the corners of solder joints due to a dramatic geometry/material change. Furthermore, a parametric finite element analysis is performed over number of geometry/material design parameters to investigate the dependence on the fatigue lives of the thermally loaded solder joint in a typical TEBGA assembly. Through the parametric design together with a rational characterization of the fatigue lives of the solder joints, the reliability characteristics of the thermally enhanced BGA package can then be effectively identified.
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Zhou, Haofei, Xin Chen, and Yumeng Li. "Design of Gradient Nanotwinned Metal Materials Using Adaptive Gaussian Process Based Surrogate Models." In ASME 2019 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/detc2019-97659.

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Abstract Inspired by gradient structures in the nature, Gradient Nanostructured (GNS) metals have emerged as a new class of materials with tunable microstructures. GNS metals can exhibit unique combinations of material properties in terms of ultrahigh strength, good tensile ductility and enhanced strain hardening, superior fatigue and wear resistance. However, it is still challenging to fully understand the fundamental gradient structure-property relationship, which hinders the rational design of GNS metals with optimized target properties. In this paper, we developed an adaptive design framework based on simulation-based surrogate modeling to investigate how the grain size gradient and twin thickness gradient affect the strength of GNS metals. The Gaussian Process (GP) based surrogate modeling technique with adaptive sequential sampling is employed for the development of surrogate models for the gradient structure-property relationship. The proposed adaptive design integrates physics-based simulation, surrogate modeling, uncertainty quantification and optimization, which can efficiently explore the design space and identify the optimized design of GNS metals with maximum strength using limited sampling data generated from high fidelity but computational expensive physics-based simulations.
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Cho, Sang-Rai, Kyeong-Ryun Kim, Seung-Uck Song, Sang-Hyun Park, Joo Sung Lee, and Jin Tae Lee. "Prediction of the Damage Extents of Ship’s Double Hull Side Structures Subjected to Lateral Collisions." In ASME 2016 35th International Conference on Ocean, Offshore and Arctic Engineering. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/omae2016-54605.

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Lateral collision tests were conducted on two steel double-hull side structure models, models DH-3 and DH-4. The models were approximately one-seventh scale of a VLCC (Very Large Crude Carrier), and the small longitudinal stiffeners of a VLCC were smeared into the shell plates. The collision tests were performed using a car crash testing facility. The striker had a knife-edge type header and its mass was 1,350 kg. The collision velocities were 7.224 m/s and 10.0 m/s for models DH-3 and DH-4, respectively. The collision test results are briefly reported in this paper. Using a commercial FEA package, numerical collision analyses were then conducted for the two new models together with another two models of one-tenth scale, which were reported elsewhere. In the numerical analyses the strain and strain-rate hardenings were considered. The convergence test was also performed to obtain an optimal mesh size. The numerical predicted damage extents were compared with those of the experiments. The prediction errors were in the range of −5.7% to +6.8%. An analytical approach was also attempted to predict the damage extents of double-hull structures subjected to lateral collisions. Compared with the experimental and numerical predictions the analytical predictions provide acceptable accuracies for the initial design stage. For a more rational ship structural design against collision, future studies are proposed to improve the prediction accuracy and reliability.
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Eno, D. R., G. A. Young, and T. L. Sham. "A Unified View of Engineering Creep Parameters." In ASME 2008 Pressure Vessels and Piping Conference. ASMEDC, 2008. http://dx.doi.org/10.1115/pvp2008-61129.

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Creep data are often analyzed using derived engineering parameters to correlate creep life (either time to rupture, or time to a specified strain) to applied stress and temperature. Commonly used formulations include Larson-Miller, Orr-Sherby-Dorn, Manson-Haferd, and Manson-Succop parameterizations. In this paper, it is shown that these parameterizations are all special cases of a common general framework based on a linear statistical model. Recognition of this fact allows for statistically efficient estimation of material model parameters and quantitative statistical comparisons among the various parameterizations in terms of their ability to fit a material database, including assessment of a stress-temperature interaction in creep behavior. This provides a rational basis for choosing the best parameterization to describe a particular material. Furthermore, using the technique of maximum likelihood estimation to estimate model parameters allows for a statistically proper treatment of runouts in a test database via censored data analysis methods, and for construction of probabilistically interpretable upper and lower bounds on creep rate. Comparisons are made to a generalization of the commonly used Larson-Miller parameterization (namely, the Mendelson-Roberts-Manson parameterization), which is comparable in complexity to the Manson-Haferd parameter, but utilizes a reciprocal temperature dependence. The general framework for analysis of creep data is illustrated with analysis of Alloy 617 and HAYNES® 230® alloy (Alloy 230) test data.
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