Journal articles on the topic 'Rabbits Spermatozoa'
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Li, Qiuyan, Jian Hou, Sheng Wang, Hong Guan, Nan Zhang, Yongfu Chen, and Xiaorong An. "Viable rabbits derived from oocytes by intracytoplasmic injection of spermatozoa from an infertile male." Zygote 17, no. 2 (May 2009): 157–62. http://dx.doi.org/10.1017/s0967199408005078.
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SummaryIntracytoplasmic sperm injection (ICSI) is an assisted fertilization technique and has been widely applied in human medicine to overcome some obstacles of infertility. However, this technology has not yet been used as a mainstream technique for animal production, including the rabbit, due to its limited success. The aim of this study was to improve ICSI techniques and establish an efficient ICSI method for rabbits. Spermatozoa used for ICSI were collected from mature New Zealand white male rabbits. They were washed two to three times with HEPES-buffered TCM199 containing 10% FBS and then mixed with 10% polyvinylpyrrolidone (PVP) prior to microinjection. Oocytes were harvested from superovulated donor rabbits after 14–15 h hCG treatment and were fertilized by microinjection of a single living spermatozoon into the ooplasm of each oocyte without additional activation treatment. A total of 317 injected oocytes resulted in the high survival rate of 86.1%. Among the surviving oocytes, 273 were placed into culture dishes for in vitro development. The fertilization, cleavage and blastocyst rates were 59.0%, 88.2% and 45.3% respectively. Furthermore, ICSI embryos were produced with spermatozoa from an infertile male rabbit, and 21 early-stage embryos (2-cell and 4-cell) were surgically transferred into the oviducts of two adult female rabbits. On day 31 after transfer, one out of the two recipients gave birth to two normal and healthy young rabbits. These results demonstrate that rabbit oocytes can be successfully activated and fertilized by the new ICSI protocol. Spermatozoa derived from infertile rabbits can successful fertilize oocytes and produce offspring by the simple ICSI technique.
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Susetyarini, Eko, and Endrik Nurrohman. "New Zealand Rabbit Spermatozoa Concentration after Giving of Beluntas Leaf Tannin Extract (Pluchea indica)." JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS 8, no. 1 (March 7, 2022): 41–52. http://dx.doi.org/10.36987/jpbn.v8i1.2473.
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The purpose of this study was to determine the concentration of spermatozoa in New Zealand rabbits after giving of various types of beluntas leaf tannin extract. This type of research is experimental research. The research design is a one-group post-test design. A sample of 16 rabbits. The sampling technique used is purposive random sampling. The research was conducted at the Chemical Engineering Laboratory of the State Polytechnic of Malang, the Laboratory of Kesimma Medica Malang, the Integrated Laboratory, and the Biology Laboratory of the University of Muhammadiyah Malang. The study was carried out from August to September 2019. The study design used a completely randomized design (CRD) with 4 treatments, namely hydrolyzed, condensed, and pure tannin extract of beluntas leaves and the control group was given aquades orally with a dose of 3 ml/kgbb with 4 replications. repetition times. The procedure of this research 1) Preparation of extract of beluntas leaf tannins, 2) Phytochemical test of extract preparations, 3) Treatment of rabbits as experimental animals, 4) Surgery and collection of rabbit spermatozoa, 5) Observation of spermatozoa using a microscope, 6) Calculating the concentration of spermatozoa. The method of data collection was by calculating the concentration of spermatozoa in each treatment using a hemocytometer and a Neubauer counting chamber using a binocular microscope. Data analysis with one-way Anova test and Duncan 5%. The results showed that the dosage of 3ml/kgbb of various types of beluntas leaf tannin extract had an effect on the spermatozoa concentration of New Zealand rabbits (sig<0.05). The findings of this study are that the best treatment that can reduce the number of spermatozoa in New Zealand rabbits is the condensed tannin extract of Beluntas leaves.
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Chrenek, P., A. V. Makarevich, and M. Simon. "Viability and apoptosis in spermatozoa of transgenic rabbits." Zygote 20, no. 1 (December 13, 2010): 33–37. http://dx.doi.org/10.1017/s096719941000050x.
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SummaryThe aim of our study was to compare the viability of sperm cells from transgenic (mWAP-hFVIII gene) or non-transgenic (normal) rabbit males as assessed by viability (SYBR-14/PI) and apoptosis (annexin V) tests. These results were evaluated using female conception rates following insemination with the respective sperm samples. No significant differences were found in concentration and motility between transgenic and non-transgenic spermatozoa. Spermatozoa from both transgenic (63.05 ± 20.05%) or non-transgenic (65.75 ± 22.15%) males, stained with SYBR-14 (green), were found to be morphologically normal. In both groups, the highest proportion of annexin V-positive sperm staining was found in the post-acrosomal part of the sperm head (8.66 and 27.53%). The percentage of sperm that stained with SYBR-14/PI or with annexin V/DAPI was correlated with liveborn in transgenic rabbits (R2 = 0.6118 and R2 = 0.2187, respectively) or non-transgenic rabbits (R2 = 0.671 and R2 = 0.3579, respectively). These data indicate that there was no difference in the viability of rabbit transgenic and non-transgenic spermatozoa when determined by both fluorescence assays.
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Adeyemi, Adenike, Christiana Oloyede, and Adedamola Adedotun. "Effect of Vernonia amygdalina leaf meal on the reproductive indices of male rabbits." Archiva Zootechnica 25, no. 2 (December 1, 2022): 63–74. http://dx.doi.org/10.2478/azibna-2022-0014.
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Abstract The study evaluated the effect of Vernonia amygdalina leaf meal on semen indices, serum testosterone and sperm reserve of male rabbits. Forty rabbit bucks were randomly assigned into four groups and fed the experimental diets containing Vernonia amygdalina (VLM) at 0, 5, 10 and 15 % levels for 84days. Reproductive indices were evaluated using standard procedures. Data obtained were subjected to analysis of variance at p<0.05. All semen in rabbits fed 0, 5 and 10 % VLM had milky colour while 14.8% light green and 85.2% milky semen colour was observed in bucks fed 15%VLM. Libido score reduced in rabbits fed VLM diets. Bucks fed 15%VLM had significantly higher semen volume (0.47ml). VLM had no significant effect on spermatozoa mass motility, progressive motility and sperm concentration. Live sperm cells significantly increased in bucks fed 5 and 10% VLM diets. VLM had significant (p<0.05) effect on spermatozoa morphology. Vernonia amygdalina leaf meal had no significant (p<0.05) impact on testosterone, testicular and epididymal indices. In conclusion, up to 10%VLM can be adopted as feed ingredient for male rabbit breeder stock without deleterious effect on reproductive indices.
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Amaduruonye, Wisdom, Joshua Nathaniel, Christopher Agboje Agida, Yakubu Ibrahim, Chidinma A. Ndukauba, and Udo Herbert. "Semen and haematological responses of rabbit bucks administered oral folic acid." Journal of Animal Science and Veterinary Medicine 6, no. 1 (February 28, 2021): 1–7. http://dx.doi.org/10.31248/jasvm2020.239.
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Some researchers have inferred that folic acid is necessary for reproduction and could enhance blood formation. Thus, a Completely Randomized Design Experiment (CRD) was conducted to evaluate the impact of oral administration of folic acid on the semen and haematological characteristics of New Zealand White rabbit bucks. The treatments designated treatment 1 (T1), treatment 2 (T2) and treatment 3 (T3) having 12 rabbits each were replicated 3 times with 4 rabbits per replicate. The ages of the 36 pre-pubertal rabbit bucks were between 2 to 3 months, and weighed approximately 2.56 kg. Three experimental diets were formulated to meet the nutrient requirements of rabbit bucks. Each rabbit buck on T1 were orally administered folic acid at 0.0 mg, T2 2.5mg folic acid and T3 5.0 mg folic, respectively. Data were collected for semen characteristics and haematology from the rabbit bucks. Data collected on different parameters were subjected to analysis of variance (ANOVA). Results showed that significant increases (p<0.05) were observed on libido, semen pH, spermatozoa progressive motility (67.40-80.20%), spermatozoa live proportion (83.01-94.12%), sperm concentration (112.24-133.80 x106/ml), total number of sperm per ejaculate (50.65-67.66 x106/ml), total viable sperm (291.58-496.69 x109/ml), normal sperm proportion (85.16-91.64%). Also, significant reductions (p<0.05) were observed on the percentage head abnormality of the spermatozoa (3.74-3.18), total abnormality (2.13-0.93), mid-piece abnormality (2.35-0.79), cytoplasmic abnormality (7.17-2.89), and total abnormality (14.84-8.35); while the haematological parameters such as haemoglobin (13.53-14.20g/dl), packed cell volume (33.00-34.96%), white blood cell (6.81-7.80 x103mm3) and the differential white blood cells improved significantly (p<0.05) following the oral administration of folic acids to the rabbit bucks. Thus, the oral administrations of folic acid at 5.0 mg per rabbit buck most significantly improved the semen characteristics, enhanced the overall spermatozoa morphology, reduced sperm cells abnormalities and also improved some haematological parameters of the rabbit bucks.
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Li, Lan, Wei Shen, Lingjiang Min, Huansheng Dong, Yujiang Sun, and Qingjie Pan. "Human lactoferrin transgenic rabbits produced efficiently using dimethylsulfoxide - sperm-mediated gene transfer." Reproduction, Fertility and Development 18, no. 6 (2006): 689. http://dx.doi.org/10.1071/rd06001.
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Transgenic animal mammary gland bioreactors are used to produce recombinant proteins. However, it is difficult to validate whether these transgenic domestic animals are able to express the recombinant protein efficiently in their mammary glands before the birth of transgenic offspring. In the present study, a simple and efficient method was established to evaluate the functionality of animal mammary gland tissue-expressed cassettes. The gene transfer vector pGBC2LF was constructed, and the expression of human lactoferrin (LF) gene was controlled by the goat β-casein gene 5′ flanking sequence. To obtain the most efficient transfection, the influence of DNA concentration, dimethylsulfoxide (DMSO) concentration, and the ratio of linear-to-circular DNA required for associating DNA with spermatozoa were evaluated. Transfection of exogenous DNA into rabbit spermatozoa was found to be efficient using 30 μg mL–1 DNA, DMSO at a final concentration of 3%, and a 3 : 1 ratio of linear-to-circular DNA, with 29 of 85 (34.1%) in vitro-fertilised embryos being transgenic. Using DMSO–sperm-mediated gene transfer (DMSO-SMGT), 89 rabbit offspring were produced, with 46 of these (57.1%) being transgenic. As mammary gland bioreactor models, 17 of 21 (81%) transgenic female rabbits could express human LF protein in their glands. During lactation of the transgenic rabbits, the highest level of human LF protein expressed was 153 ± 31 μg mL–1, and the mean expression level in all of the transgenic rabbits was 103 ± 20 μg mL–1 in the third week, declining gradually after this time. Our results demonstrate that transgenic rabbits produced by DMSO–SMGT were able to express human LF protein in the correct tissue.
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Zyuzyun, A. B. "CYTOMORPHOLOGICAL RESEARCH OOCYTE RABBIT IN THE PROCESS EMBRYOGENESIS IN VITRO." Animal Breeding and Genetics 52 (November 1, 2016): 181–86. http://dx.doi.org/10.31073/abg.52.24.
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Introduction. Using genetic potential of rabbit ovaries and studying patterns of meiotic maturation of gametes in female in vitro is a basis for success in cloning and the creation of transgenic animals, so there is a need for in-depth study of cytomorphological characteristics of oocytes during in vitro embryogenesis. The aim of the research is cytomorphological study of oocytes during embryogenesis, derived from matured rabbits’ ovaries and before the sexual cycle. Materials and methods of research. The ovaries of the rabbits (n = 8) aged 4 months and rabbits aged 11 months, coming into heat (n = 10) were used in the study. All the ovaries derived from females, were at follicular growth phase. Rabbits’ oocyte-cumulus complexes were cultured in vitro during 24 hours in plastic Petri dishes (25 - 30 OCC per ml) in the medium for maturing – 199 with Earle’s solution (Sigma, M 5017), supplemented by 20% heat-inactivated (56ᵒC, 30 min.) homemade estrous cow serum, 0.068 mg/ml kanamycin sulfate, 0.11 mg/ml sodium pyruvate and 0.1 mg/ml glutamine. Granulosa cells derived from the antral follicles without atresia evidence were necessarily added in the culturing medium in amount of 3–5х106 per ml. Received in vitro the ova were fertilized by freshly derived rabbit’s epididymal spermatozoa. Results. As a result of extracting oocytes from all the ovaries (n = 18), 245 OCC were received, including 115 OCC from eight rabbits’ ovaries before the sexual cycle and 130 OCC from ten ovaries of mature rabbits. Analysis of cytomorphological studies found more (P < 0,05) oocyte-cumulus complexes can be received from rabbits’ ovaries during puberty being suited for cultivation than from mature rabbits’ ovaries. After in vitro culturing 85,5 % of OCC (47 of 55) derived from rabbits’ ovaries during puberty and 75.6% of OCC (62 of 82) – from mature rabbits’ ovaries reached MII meiosis. It was revealed the level of in vitro maturation of oocytes was 10 % higher in the group derived from rabbits’ ovaries at puberty, compared with the group derived from mature rabbits’ ovaries. Ripened outside a body the oocytes were fertilized in vitro by freshly derived rabbit’s epididymal spermatozoa. The embryos developed in the both groups, but with a significant difference in the level of division. Cytomorphological research found that the level of 2-4-cell embryos formation in the group of oocytes derived from rabbits at puberty was 68,1% and in group of gametes from mature rabbits – only 46.8 % (P < 0.05). 22.2 % of embryos on average developed to the morula stage in vitro. In terms of embryo development to early morula stage significant difference between the groups wasn’t found. The significant difference between the study groups in the number of zygotes have not passed division block (P < 0.05) was noted. In the group of oocytes derived from mature rabbits’ ovaries the zygotes which have not divided were 23.8 % more. Conclusions. It was found that more oocyte-cumulus complexes (P < 0,05) were received from rabbits’ ovaries during puberty, being suited for in vitro cultivation, than from mature rabbits’ ovaries. And oocytes with degeneration signs, being unsuited for in vitro cultivation, were received more (P < 0.05) from mature rabbits’ ovaries. The level of maturation also was 10 % higher in the group of OCC derived from rabbits’ ovaries at puberty. So, for biotechnology research as oocyte donors more effective is use of rabbits during puberty, which have not yet begun sexual cycle, because significantly more (P < 0,05) fully-fledged oocytes cumulus complexes, being suited to culture outside a body, can be derived from their ovaries which will provide greater percentage of preimplantation embryos.
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Roychoudhury, Shubhadeep, Peter Massanyi, Jaroslav Slamecka, Ivan Chlebec, Jozef Trandzik, Jozef Bulla, Aly B. Okab, Taha A. Taha, Mohamed H. Salem, and Mostafa A. Ayoub. "In vitrogossypol induced spermatozoa motility alterations in rabbits." Journal of Environmental Science and Health, Part B 44, no. 7 (September 16, 2009): 730–41. http://dx.doi.org/10.1080/03601230903163905.
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Tvrdá, Eva, Michal Ďuračka, Marek Halenár, and Attila Kántor. "In Vitro Effects of Enterococcus Faecalis and Selected Biomolecules on the Motility of Rabbit Spermatozoa." Contemporary Agriculture 66, no. 3-4 (December 20, 2017): 22–31. http://dx.doi.org/10.1515/contagri-2017-0015.
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SummaryThis study assessed the potential efficiency of selected biologically active substances on the motility behavior of rabbit spermatozoa subjected to in vitro induced E. faecalis contamination. Semen samples were collected from 10 male rabbits and the presence of E. faecalis was confirmed using MALDI-TOF Mass Spectrometry. For the in vitro experiments rabbit spermatozoa were resuspended in the presence of 0,3 McF E. faecalis and different concentrations of selected biomolecules (resveratrol - RES, quercetin - QUE, curcumin - CUR, epicatechin - EPI, isoquercitrin - IZO). Sperm motility was assessed using the computer-aided sperm analysis at 0h, 2h, 4h, 6h and 8h. The presence of E. faecalis significantly decreased the motility (P<0.001) when compared to the untreated Control starting at 2h and maintaining this negative impact throughout the entire in vitro culture. Meanwhile, the motility was significantly higher in the experimental samples subjected to E. faecalis together 5 μmol/L RES (P<0.05), 10 μmol/L QUE (P<0.05) as well as 1 μmol/L (P<0.01) and 10 μmol/L CUR (P<0.05) when compared to the Positive Control (4h). No biomolecule was able to maintain the motion comparable to the Negative Control, and none was effective against the rapid decline of sperm motility caused by the presence of E. faecalis during later stages of the in vitro experiment (6h and 8h). We may conclude that RES, QUE and CUR may provide a selective advantage to spermatozoa in the presence of E. faecalis, particularly during short-term rabbit semen handling.
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Srivastav, Archana, Balvir Singh, Abhishek Chandra, Farrukh Jamal, Mohammad Y. Khan, and Sunil R. Chowdhury. "Partial characterization, sperm association and significance of N- and O-linked glycoproteins in epididymal fluid of rhesus monkeys (Macaca mulatta)." Reproduction 127, no. 3 (March 2004): 343–57. http://dx.doi.org/10.1530/rep.1.00119.
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The present study investigated regional modifications of glycosylation status, sperm association and functional significance of N- and O-linked glycoproteins in epididymal luminal fluid of the rhesus monkey (Macaca mulatta). The predominant glycoproteins of the epididymal luminal fluid that increase in the extent of glycosylation or unmasking of exposed epitopes in a region-specific, maturation-dependent manner, included those of 150, 116, 68, 64, 58 (N- and O-linked) and 170 kDa (O-linked). The higher expression of 40 (N-linked), 38 (N- and O-linked) and 60, 56 and 33 kDa (O-linked) glycoproteins in the proximal caput epididymal fluid was followed by alteration or reorganization of 60, 38 and 33 kDa (O-linked) glycoproteins in the distal segments of the epididymis. The association of epididymal fluid glycoproteins with maturing spermatozoa was identified by generating polyclonal antiserum against monkey caudal sperm membrane in female albino rabbits. The antiserum crossreacted strongly with 58 and 33 kDa epididymal fluid glycoproteins of monkeys and also reacted with 116, 68, 58, 56 and 33 kDa glycoproteins from Triton X-100 extracts of human spermatozoa, indicating the presence of antigenically related components in both species. The functional significance of epididymal fluid glycoproteins in sperm functions was investigated by raising antiserum against a heavily glycosylated 58 kDa glycoprotein (MEF1) of caudal epididymal fluid, which crossreacted with the Triton X-100 extracts of epididymal spermatozoa of monkey and ejaculated human spermatozoa on immunoblots. In an in vitro micro-sperm agglutination assay, anti-MEF1 serum agglutinated both rat caudal epididymal spermatozoa and human spermatozoa. MEF1 seemed to be involved in fertilization as demonstrated by inhibition of fertility (100%) in female albino rabbits and rats immunized with this protein. A sperm-agglutinating 58 kDa glycoprotein of rhesus monkey epididymis with functional significance in fertility was identified, thus indicating that it is a potential candidate for contraceptive vaccine development.
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Ata, A., O. Yildiz-Gulay, S. Güngör, A. Balic, and M. S. Gulay. "The effect of carob (Ceratonia siliqua) bean extract on male New Zealand White rabbit semen." World Rabbit Science 26, no. 3 (September 28, 2018): 209. http://dx.doi.org/10.4995/wrs.2018.10154.
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<p>The carob tree (Ceratonia siliqua) grows naturally in the Mediterranean region. The empiric use of carob cures for their aphrodisiac properties is very common in Turkey. Thus, the experiment was conducted to determine the effects of carob bean extracts on some reproductive parameters in male New Zealand White rabbits. During the adaptation period (stage 1), 6-8 mo old rabbits were trained in semen collection for 30 d. At the beginning of the treatment period (stage 2), rabbits were assigned randomly to 2 groups of 8 animals each. For a period of 49 d (1 spermatogenesis duration), one group was treated with a daily oral dose (10 mL) of carob extract and the other group received the corresponding volume of tap water. Semen was collected weekly. Semen samples taken at week 1 and 7 were analysed separately. At the beginning of stage 2, no differences were observed in the volume and pH of the ejaculate, sperm concentration, percentage of motility, percentage of live spermatozoa, percentage of sperm plasma membrane integrity, plasma concentration of testosterone, and seminal plasma protein levels between the control and carob extract treated animals. Similarly, at the end of stage 2, there were no differences in the volume and pH of the ejaculate, motility percentage, the percentage of live spermatozoa, percentage of sperm plasma membrane integrity, and the seminal plasma protein levels between the control and the carob extract treated animals. However, sperm concentration (P<0.05), plasma concentration of testosterone (P<0.05), and percentage of change in spermatozoa concentration (P<0.02) between groups were affected at the end of stage 2. The data suggested that the use of carob cures prepared by boiling carob fruit could have beneficial influences on sperm concentration in rabbits.</p>
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Theau-Clément, M., N. Michel, J. Esparbié, and G. Bolet. "Effects of artificial photoperiods on sexual behaviour and sperm output in the rabbit." Animal Science 60, no. 1 (February 1995): 143–49. http://dx.doi.org/10.1017/s1357729800008249.
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Male rabbits aged from 4 to 4·5 months were subjected to a 8L: 16D photoperiod for 4 weeks and were then split into two groups. One group of13 males was maintained at 8L: 16D (group 8). The other included 16 males and was submitted to 16L: 8D (group 16). Two successive ejaculates zvere collected and analysed once a week for 6 months. Animals from group 8 were sexually more active and ejaculated significantly larger volumes of semen than those from group 16. Performance of males from group 16 was better for all other measurements, whether these were qualitative (motility, percentage of live spermatozoa) or quantitative. Averages for the total number of spermatozoa and number of live spermatozoa per ejaculate were significantly greater in males submitted to 16L: 8D (509 and 408 v. 452 and 344 × 106 spermatozoa, respectively). However the testes of rabbits in group 8 had a significantly greater volume than those in group 16 (22·3 v. 17·4 cm3, P < 0·001). After the animals were slaughtered when 11 months of age, the volume and weight of the testes and the testis and epidydimis reserves did not differ significantly between groups. Under our experimental conditions, the sperm output of bucks exposed to 16 h of daylength was greater than that of bucks exposed to 8 h of daylength but in vivo and post-mortem measurements did not reflect this difference.
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Carvalho, M., L. Mateus, F. Afonso, S. Van Harten, L. Alfaro Cardoso, D. A. Redmer, and G. Ferreira-Dias. "Testicular angiogenic activity in response to food restriction in rabbits." REPRODUCTION 137, no. 3 (March 2009): 509–15. http://dx.doi.org/10.1530/rep-08-0199.
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The objective of this study was to evaluate the effects of two different levels of food restriction on testicular angiogenic activity, microvascularization, tissue growth, and regression, using the rabbit as a study model. The rabbits (Oryctolagus cuniculus cuniculus) were randomly assigned to a control group (A,n=5), fedad libitum, and to groups B (n=5) and C (n=5), with two different levels of food restriction. Food restriction was responsible for a 21.2% decrease in body weight in group B and 34.7% in group C. Testis explants were cultured for 24 h and conditioned media were tested for their ability to stimulate mitogenesis of bovine aortic endothelial cells (BAEC). There was an increase in testicular microvascular area and mitogenesis of BAEC in group C rabbits. Despite no change in testicular DNA concentration among groups, food restriction decreased both RNA and protein compared with control. No treatment differences in the percentage of seminiferous tubules filled with all stages of spermatogenesis (spermatogonia, spermatocytes, and spermatids) and spermatozoa, as well as the area occupied by seminiferous tubules, were observed. Nevertheless, serum testosterone was markedly less in group C compared with groups A and B. These results suggest that angiogenesis may play a role in overcoming testicular nutritional impairment in rabbits subjected to food restriction.
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Valdivia, M., T. Sillerico, A. De Ioannes, and C. Barros. "Proteolytic activity of rabbit perivitelline spermatozoa." Zygote 7, no. 2 (May 1999): 143–49. http://dx.doi.org/10.1017/s0967199499000507.
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Acrosin, an acrosomal serine protease, has been associated with binding of spermatozoa and their penetration through the zona pellucida. This study was aimed at determining whether the remaining proacrosin/acrosin system on rabbit perivitelline spermatozoa still has proteolytic activity and whether this activity is involved in further penetration of unfertilised rabbit eggs. Eight hundred and sixty-five rabbit perivitelline spermatozoa were evaluated by the gelatin-substrate film technique for the detection of acrosin on individual spermatozoan. Fifteen per cent of the studied spermatozoa showed small digestion halos on the gelatin film. The proteolytic activity of rabbit perivitelline spermatozoa was inhibited in the presence of 1 mg/ml of soybean trypsin inhibitor (SBTI) or with 20 μg/ml of a mixture of the monoclonal anti-proacrosin/acrosin antibody. In vitro fertilisation occurred in 21.8% of rabbit oocytes co-incubated with perivitelline spermatozoa and was completely inhibited when oocytes were incubated with 600 μg/ml of a mixture of three anti-acrosin monoclonal antibodies (ACRO-A8C10, ACRO-C2B10 and ACRO-C5F10). Inseminations in the presence of anti-cholera monoclonal antibody (irrelevant to spermatozoa) resulted in 17.6% fertilisation. These results support the idea that the residual proacrosin/acrosin system in perivitelline spermatozoa might be involved in spermatozoal binding and/or second penetration through the zona pellucida.
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Diaz-Fontdevila, M., W. Peña, and E. Bustos-Obregón. "Experimental hypercholesterolaemia in rabbits. Effect on lipid domains in homologous spermatozoa." Andrologia 30, no. 1 (April 24, 2009): 15–22. http://dx.doi.org/10.1111/j.1439-0272.1998.tb01377.x.
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YPSILANTIS (Π. ΥΨΗΛΑΝΤΗΣ), P., Ph SARATSIS (Φ. ΣΑΡΑΤΣΗΣ), and S. SAMOUILIDIS (Σ. ΣΑΜΟΥΗΛΙΔΗΣ). "Rabbit semen fertility after heterospermic insemination with spermatozoa of different capacitation time." Journal of the Hellenic Veterinary Medical Society 49, no. 2 (January 31, 2018): 143. http://dx.doi.org/10.12681/jhvms.15766.
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The influence of mixing heterogeneous sperm populations in rabbit semen fertility was studied. Eighty female rabbits which were divided in 4 groups (1,2,3 and 4) in = 20.) and 3 males of proven fertility were employed. The animals of group 1,2 and 3 were inseminated with semen from male A j, A2 and A3, respectively, while those of group 4 were inseminated with a mixture containing equal number of progressively motile spermatozoa from each of the above mentioned males ( A 1 + 2 + 3 ) (heterospermic insemination). Animals of each group were divided into 4 subgroups according to the insemination time (15,10, 5 and 0 hours prior the expected ovulation). In each group, differences were observed (P<0.05) between subgroups at the percentage of animals that delivered, indicating differences between males at the time and the duration of sperm capacitation in vivo. Based on the percentage of the animals that delivered and the litter size, semen fertility was improved after the application of heterospermic insemination, at all insemination times. This improvement was attributed to the extension of the time during which capacitated spermatozoa were present in the female genital tract due to the mixture of heterogeneous sperm populations of different capacitation time.
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Baccetti, B., A. G. Burrini, J. S. Chen, G. Collodel, D. Giachetti, F. Matteucci, M. G. Menesini-Chen, E. Moretti, P. Piomboni, and C. Sensini. "Evaluation of the antifertility activity of the broom Spartium junceum in the mammalian male." Zygote 1, no. 1 (February 1993): 71–78. http://dx.doi.org/10.1017/s0967199400001313.
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SummaryMale adult rabbits and rats treated with Spartium junceum showed a significant decrease in fertility, demonstrated by a lower number of pregnancies. The target of the drug seems to be the acrosomal protease system, the activity of which appears greatly reduced, while the morphology of testicular cells and epididymal spermatozoa is only partially affected. The antifertility effect is completely reversible.
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Togun, V. A., G. O. Farinu, O. O. Ojebiyi, S. A. Ayorinde, and C. O. Majaro. "Growth and Testicular Spermatozoa Reserve Potential Of Pre-pubertal Rabbits Fed With Diet Containing 16% Bovine Rumen Digesta-BIood Meal Mixture (50:50) In combination With 18% Cooked Macuna Bean Meal." Nigerian Journal of Animal Production 36, no. 1 (January 1, 2021): 175–87. http://dx.doi.org/10.51791/njap.v36i1.1393.
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The growth response of pre-pubertal rabbits and their gonadal sperm reserve potential at pubertal stage when fed diets containing a combination of 16% (50-50) bovine rumen digesta-blood meal (BRDBM) mixture and 18% cooked mucuna bean (CMBM). This was in replacement of 500/0 of the maize and 50% of the groundnut cake of a pre-pubertal control diet. Twenty-four male rabbits of mixed breed aged between 6-8 weeks and body weight range 900-915g were randomly allocated to two diets: the control (diet 1) and the experimental (diet 2). Daily feed intake (72.4±7.5g vs 64.0±9.1g), total weight gain (518±1g Vs 406±18g) and Terminal live weight (1427±48g vs 1317±410 were significantly (P <0.05) higher in the control than experimental group. Feed to gain ratio in group I was marginally lower (7.5±2.6g Vs 8.5±1.2g) than for diet 2. However, dressing percentage (68.6±7.00/6 Vs 75.4±9.0%) was significantly higher in the rabbits on diet 2 than diet l. The cost per kg of diet Vs as well as the cost per kg live weight gain (N352.6±O.l VsN344.5H).l) was significantly lower for diet 2 than diet l. The paired testes weight (1.8±0.6g Vs 1.9±0.40, daily sperm production (3.6x 108 Vs 3,8x 108) and testicular spermatozoa reserve were marginally higher in rabbit on diet 2 (experimental) than diet 1 (control). The paired epididymal weight for rabbits on diet 2 was significantly (P <0.05) higher (0.6±0.3g Vs 1.2±0.30 than those on diet l, indicating more efficient sperm storage. The hematological measured were not affected (P>O. 05) by the dietary treatments. The non-significant difference (P>O. 05) in the efficiency of sperm production and sperm reserve between the two diets confirmed that the inclusion of these materials, in the diet of rabbits supports the ventures Of increasing animal protein sources through cheaper rabbits production process in Nigeria
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Reddy, K. V. R., C. Aranha, S. M. Gupta, and R. D. Yedery. "Evaluation of antimicrobial peptide nisin as a safe vaginal contraceptive agent in rabbits: in vitro and in vivo studies." Reproduction 128, no. 1 (July 2004): 117–26. http://dx.doi.org/10.1530/rep.1.00028.
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In the midst of the global epidemics of both unwanted pregnancies and sexually transmitted infections (STIs), options that provide protection are ideal. In the present study, nisin, a known antimicrobial peptide, was evaluated for safety and contraceptive potentialin vitroandin vivoin the rabbit. A concentration of 400 μg nisin per ml was found to be spermicidalin vitro, and the effect was dose and time dependent.In vivostudies indicated that intravaginal application of 1 mg nisin blocked conception in rabbits. Repeated application of nisin (50 mg/animal per day) in rabbits for 14 consecutive days did not cause local inflammation or damage to the vaginal epithelium. In addition, the rate of diffusion of nisin into the blood via the vaginal mucosal epithelium, and its clearance from the circulation was found to be rapid. No treatment-related changes were observed in the reproductive performance of rabbits after cessation of treatment. Furthermore, no changes were observed in the gestation period, subsequent growth and survival of neonates in these animals. When male rats were given nisin orally for 13 consecutive weeks, no effect was observed on reproductive performance. The number of pups born, survival and growth of pups were unaltered. The affinity studies of nisin revealed that spermatozoa are more susceptible to nisin than red blood cells and vaginal epithelial cells. We suggest that nisin with spermicidal and antimicrobial properties could serve as a safe vaginal contraceptive for future therapeutic interventions in STIs.
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BESSEBOUA, Omar, and Abdelhanine AYAD. "EFFECT OF SACCHAROMYCES CEREVISIAE FEED SUPPLEMENTATION ON HAEMATOLOGY AND REPRODUCTIVE PARAMETERS FOR ALGERIAN RABBITS." Journal of Applied Life Sciences and Environment 186, no. 2 (June 22, 2021): 111–22. http://dx.doi.org/10.46909/journalalse-2021-011.
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This study aims at investigating the effect of Saccharomyces cerevisiae (SC) supplementation on reproductive performance, haematological parameters and fertility of rabbits under Algerian conditions. The animals were divided into three groups and received the same feed ration during the experimental period. The control group received a basal diet without feed additives (Group#0) and the two yeast SC groups received 0.3 and 0.6 g/day per head (Group#1 and Group#2, respectively). Semen and blood samples were collected for determination of semen parameters and haematology. The weights of rabbits treated with SC 0.3 g/day were statistically significantly different (P< 0.05) from the control groups and group treated with SC 0.6 g/day. There were significant differences between the treatment groups for (RBCs), haemoglobin (HGB), haematocrit (HCT) and mean corpuscular haemoglobin (MCH) values, with higher values in rabbits supplemented with SC 0.3 g/day and 0.6 g/day, compared to those in the control group. The scrotal diameter did not differ between the dietary treatments. When compared with the control group, feeding rabbits graded levels of SC resulted in an increase in the average semen volume, mass motility and individual motility at day 51 of the experiment. On the other hand, the sperm concentration was significantly lower (P< 0.05) in rabbits supplemented with SC 0.3 g/day and 0.6 g/day during the two months compared to that in the control group. The spermatozoa mortality rate was lower for the rabbits supplemented with SC 0.3 g/day and 0.6 g/day (15.7% and 11.4%, respectively), compared to that in the control group (24%). In conclusion, this study has shown that inclusion of SC 0.3 g/day and 0.6 g/day in the diets of rabbit has positive effects on body weight and sperm analysis. Moreover, it increases the level (RBCs), haemoglobin (HGB), haematocrit (HCT) and mean corpuscular haemoglobin (MCH).
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Vasicek, Jaromir, Juraj Pivko, and Peter Chrenek. "Reproductive Performance of New Zealand White Rabbits after Depletion of Apoptotic Spermatozoa." Folia Biologica 62, no. 2 (May 30, 2014): 109–17. http://dx.doi.org/10.3409/fb62_2.109.
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Chandra, Abhishek, Kokattam Rama Srinivasan, Farrukh Jamal, Puroshottam Kumar Mehrotra, Ram Lakhan Singh, and Archana Srivastav. "Post-translational modifications in glycosylation status during epididymal passage and significance in fertility of a 33 kDa glycoprotein (MEF3) of rhesus monkey (Macaca mulatta)." REPRODUCTION 135, no. 6 (June 2008): 761–70. http://dx.doi.org/10.1530/rep-07-0525.
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The present study reports data on post-translational modifications in the glycosylation status during epididymal passage and significance in fertility of a 33 kDa glycoprotein of rhesus monkey (Macaca mulatta), designated as MEF3 (monkey epididymal fluid protein 3). MEF3 exhibited strong affinity for N-linked α-d-mannose groups and O-linkedN-Ac-galactosamine linkages in epididymal fluids and exhibited moderate affinity forN-Ac-glucosaminylated (wheat germ agglutinin), fucosylated (Tetragonolotus purpurea), andN-Ac-galactosamine (peanut agglutinin) residues on more mature corpus and caudal spermatozoa in a maturation-dependent manner on Western blots probed with specific biotinylated lectins. Polyclonal antiserum raised against affinity-purified MEF3 from caudal epididymal fluid (CEF) cross-reacted specifically with CEF and caudal sperm membrane of macaque and with Triton X-100 extract of ejaculated human spermatozoa, suggesting the existence of antigenically related components in both species. The tangled agglutination caused by anti-33 kDa serum of human spermatozoa, along with localization of MEF3 on entire sperm surface of epididymal and testicular sperm of monkey and human spermatozoa, suggest the significance of MEF3 in sperm function. The 100% inhibition of fertility of immunized female rabbits with this proteinin vivoand inhibition of human sperm penetration in zona-free hamster eggsin vitrosuggests the functional significance of MEF3 in fertility. Together, these results clearly indicate that MEF3 has potential significance as a target for antibodies that inhibit sperm function and fertility.
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Roychoudhury, Shubhadeep, Jozef Bulla, Peter Massanyi, and Manabendra Dutta Choudhury. "Copper affects spermatozoa motility, morphology and cell membrane integrity in rabbits in vitro." Journal of Biotechnology 150 (November 2010): 451. http://dx.doi.org/10.1016/j.jbiotec.2010.09.655.
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Jimoh, O. A., E. O. Ewuola, and P. Acheneje. "Sexual urge, semen quality and seminal oxidative markers of rabbit bucks of different reproductive stages in response to peak of heat stress in Southwest of Nigeria." Nigerian Journal of Animal Production 48, no. 2 (March 2, 2021): 27–37. http://dx.doi.org/10.51791/njap.v48i2.2937.
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Rabbits of three reproductive stages were evaluated for their reproductive superiority during peak thermal comfort in Ibadan, southwest Nigeria. Within rabbit population in the study area, bucks were categorized into age groups from the parity records of the farm. Pubertal rabbits (4-5 months old), Mature rabbits (7-9 months old), Adult rabbits (above 1-year-old). Animals were housed individually and allotted randomly into experimental units. This investigation was carried out within February and March when the average daily temperature humidity index (THI) is at the peak. Thirty rabbit bucks per age group, housed individually and randomly allotted to experimental units were used for this study. All bucks were made to serve an artificial vagina, libido was evaluated, and semen collected were assessed for semen characteristics, seminal biochemical and oxidative stress indices weekly from the ninth week. The result obtained revealed that mature bucks had significantly (p<0.05) highest semen volume, mass activity, motility, sperm concentration, total motile and total live spermatozoa among the three age groups. The effect of heat stress was most severe in pubertal bucks as they recorded the least y (p<0.05) semen quality among the three age groups. Seminal lipid peroxidation was significantly (p<0.05) higher in mature and adult bucks compared with pubertal bucks. Seminal total antioxidant capacity was significantly (p<0.05) highest in pubertal bucks but the least value was obtained in mature bucks. It can be concluded that pubertal rabbits possess the best antioxidant stability among the three age group but possessed least semen quality compared with adult and mature buck. Des lapins de trois stages reproducteurs ont été évalués pour leur supériorité reproductrice pendant le maximum de confort thermique à Ibadan, au sud-ouest du Nigeria. Dans la population de lapins dans la zone d'étude, les mâles ont été classés en groupes d'âge à partir des registres de parité de la ferme. Lapins pubertés (4-5 mois), lapins matures (7-9 mois), lapins adultes (au-dessus de 1 an). Les animaux ont été logés individuellement et attribués au hasard dans des unités expérimentales. Cette enquête a été menée en février et mars, lorsque l'indice quotidien moyen d'humidité de la température (THI) est au sommet. Trente mâles de lapin par groupe d'âge, logés individuellement et aléatoirement attribués à des unités expérimentales ont été utilisés pour cette étude. Tous les mâles ont été faits pour servir un vagina artificiel, la libido a été évaluée, et le sperme recueilli ont été évalués pour des caractéristiques de sperme, des indices biochimiques et oxydants séminaux de stress hebdomadaires de la neuvième semaine. Le résultat obtenu a révélé que les mâles matures avaient significativement (p<0.05) le volume de sperme le plus élevé, l'activité de masse, la motilité, la concentration de sperme, le motile total et le spermatozoa vivant total parmi les trois groupes d'âge. L'effet du stress thermique a été le plus grave chez les mâles pubertés, car ils ont enregistré la moins de qualité de sperme y (p<0.05) parmi les trois groupes d'âge. La peroxydation séminale de lipide était sensiblement (p<0.05) plus élevée dans les mâles mûrs et adultes comparés aux mâles pubertals. La capacité antioxydante totale séminale était significativement (p<0.05) la plus élevée dans les mâles pubertals mais la valeur la moins élevée a été obtenue dans les mâles mûrs. On peut conclure que les lapins pubertés possèdent la meilleure stabilité antioxydante parmi le groupe d'âge trois mais ont possédé la qualité inférieure de sperme comparée à l'argent adulte et mûr.
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Hirabayashi, M., M. Kato, and S. Hochi. "314 FULL-TERM DEVELOPMENT OF RAT OOCYTES MICROINSEMINATED WITH FREEZE-DRIED SPERMATOZOA." Reproduction, Fertility and Development 17, no. 2 (2005): 307. http://dx.doi.org/10.1071/rdv17n2ab314.
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Since freeze-dried spermatozoa can be stored at ambient or refrigerated temperature, the costs required for maintenance and shipping of spermatozoa can be reduced. To date, viable offspring in mice (Wakayama and Yanagimachi 1998 Nat. Biotech. 16, 639) and rabbits (Liu et al. 2004 Biol. Reprod. 70, 1776) have been produced by intracytoplasmic sperm injection (ICSI) using freeze-dried samples. The objectives of the present study were to examine whether freeze-dried rat spermatozoa can participate in full-term development by ICSI, and whether sonication prior to freeze-drying of the spermatozoa influences the offspring rate. Spermatozoa from cauda epididymides of Sprague-Dawley (SD) rats were collected in 10 mM TRIS/HCl buffer supplemented with 50 mM NaCl and 50 mM EGTA. A 2 × 3 factorial-designed experiment was conducted. The sperm suspensions were either sonicated for 10 s using a 10% power output from an ultrasonic cell disruptor or not sonicated. The sperm suspensions were then processed for freeze-thawing (100-μL sample in 1.0-mL cryotube was cooled in liquid nitrogen vapor, stored at -196°C for 48 h, and thawed in a 25°C water bath) and freeze-drying (100-μL sample in 1.5-mL polypropylene tube was frozen in liquid nitrogen for 20 s, lyophilized for 6 h by a freeze-drying apparatus, stored at 4°C for 48 h, and rehydrated with 100 μL ultra pure water), or were subjected to immediate use for ICSI. The sperm heads were microinjected into denuded SD oocytes using a piezo-driven micropipette 2–4 μm in diameter, as described previously (Hirabayashi et al. 2002 Transgenic Res. 11, 221). The presumptive zygotes were transferred into oviducts of pseudopregnant Wistar female rats. The in vivo developmental potential of rat oocytes microinseminated with fresh, freeze-thawed, and freeze-dried spermatozoa is shown in the table below. Viable rat offspring were produced in all six experimental groups, with the offspring rates at 2.5–35.0%. Sonication treatment of rat spermatozoa to induce membrane disruption and tail/midpiece dissociation from the heads was effective in increasing the offspring rate after ICSI. The positive effect of sperm sonication may be explained as facilitating decondensation of sperm heads by membrane disruption in the spontaneously activating rat oocytes. Thus, successful participation of freeze-dried rat spermatozoa into full-term development was demonstrated by applying the ICSI. Table 1. In vivo development of rat oocytes microinseminated with fresh, freeze-thawed, and freeze-dried spermatozoa
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Ewuola, E. O., and G. N. Egbunike. "Effects of dietary fumonisin B1 on the onset of puberty, semen quality, fertility rates and testicular morphology in male rabbits." REPRODUCTION 139, no. 2 (February 2010): 439–45. http://dx.doi.org/10.1530/rep-09-0077.
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The influences of dietary fumonisin B1(FB1), a metabolite ofFusarium verticillioides, on the onset of puberty, semen quality, fertility rates and testicular morphology in male rabbits (bucks) were studied. Forty male rabbits were randomly assigned and fed four diets containing 0.13, 5.0, 7.5 and 10.0 mg FB1/kg, constituting diets 1 (control), 2, 3 and 4 respectively, for a period of 175 days in a completely randomized design. During the last week of the feeding trial, two untreated female rabbits were mated to each of the four treated bucks per treatment to assess the fertility rate of the treated bucks. Onset of puberty in animals fed diets 3 and 4 was significantly (P<0.05) delayed by some 9–12 days. The weight at puberty, sperm concentration and total sperm/ejaculate were not significantly influenced by the dietary FB1. Sperm mass activities, motility and live spermatozoa of the rabbits' semen significantly (P<0.05) declined with an increase in the dietary FB1. The highest sperm cell abnormalities were recorded in the animals fed 10.0 mg/kg FB1, while the least was observed in the control animals. The conception rate, litter size and embryo survival rate were statistically the same among the dietary treatments. Embryo mortality was significantly (P<0.05) higher in rabbits fed diets 3 and 4 than in others. Testicular elements were significantly (P<0.05) impaired by the toxin in rabbits fed 7.5 and 10.0 mg FB1/kg. This suggests that LOAEL of 7.50 mg/kg FB1delayed puberty, impaired semen quality and spermatogenesis and induced embryo mortality without a statistically adverse effect on the fertility rates of male rabbits.
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Imeer, Ali Thoulfikar A., and Abdul Amir H. Kadhum. "Morphological, Histological, and Ultrastructural Changes in Epididymis after a Vasectomy." Journal of Hunan University Natural Sciences 49, no. 4 (April 30, 2022): 182–92. http://dx.doi.org/10.55463/issn.1674-2974.49.4.18.
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The long-term effect of a vasectomy was investigated in this work. Twenty sexually mature New Zealand white male rabbits (Oryctolagus cuniculus) of proven fertility were used. The maturity of these rabbits was determined by the age of about 14 months from birth, palpation of genitalia, and examination of semen samples. The animals were divided into four groups: four rabbits were used as controls, and sixteen rabbits were vasectomized. After six months after vasectomy, we start taking a sample by exposing each group in one month after the previous one. The operated ducts revealed an increase in size in 7-8 months after vasectomy. The initial and terminal segments appeared greatly swollen and distended with fluid, resulting in the whole epididymis's increasing weight. Histological changes included a drop in the height of the lining epithelium and microvilli of the epididymis. After eight months of vasectomy, dilatation in the epididymal tubules increased its diameters, especially in the terminal segment. This caused an increase in the thickness of intertubular connective tissue. In addition, there was distension due to the intratubular pressure increase. This resulted in the tubular wall rupture and extravasation of spermatozoa into the interstitial tissue after 6-7 months from vasectomy. Eight months later, the epithelial lining was returned. The examination of morphological changes due to vasectomy was also investigated.
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Veeramachaneni, D. N. R., J. S. Palmer, R. P. Amann, C. M. Kane, T. T. Higuchi, and K.-Y. F. Pau. "Disruption of sexual function, FSH secretion, and spermiogenesis in rabbits following developmental exposure to vinclozolin, a fungicide." Reproduction 131, no. 4 (April 2006): 805–16. http://dx.doi.org/10.1530/rep.1.01048.
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We studied sequelae of prenatal plus infantile exposure of male rabbits to vinclozolin, because it is ingested by women and children. Female Dutch-Belted rabbits (7–10/group) were treated daily per orum from gestation day 15 through post-natal week 4 to provide 0, 7.2, or 72 mg vinclozolin/kg dam’s body weight/day. Vinclozolin had no effect on maintenance of pregnancy, growth of pups, age at testicular descent or weight of organs. Concentrations of serum LH or testosterone at 6, 12, or 24 weeks of age were unaffected. However, FSH was lower (P< 0.05) in both vinclozolin groups at all three ages. Following injection of GnRH at 12 or 24 weeks, the increase in FSH was less (P< 0.05) in both vinclozolin groups, as was testosterone at 12 weeks of age. After full sexual maturity, 2 of 7 low dose rabbits were uninterested in female or male teasers and never achieved erection or ejaculation. Overall, rates of ejaculation failure were: control 0% (0/48), low dose 29% (12/42), and high dose 5% (3/60). Daily sperm production per gram of testis and total number of sperm per ejaculate in both vinclozolin groups were similar (P> 0.1) to controls. However, semen from vinclozolin rabbits contained over two times more (P< 0.05) morphologically abnormal spermatozoa, mostly nuclear and acrosomal defects, than semen from controls. Seminiferous tubules with degenerative changes were more frequent (P< 0.05) in vinclozolin rabbits than in controls. Lesions included syncytia of spherical spermatids and desquamation of germ cells. Hence, developmental exposure to vinclozolin caused presumably permanent changes in copulatory ability, secretion of FSH, and spermiogenesis.
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Gliozzi, T. M., L. Zaniboni, A. Maldjian, F. Luzi, L. Maertens, and S. Cerolini. "Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets." Theriogenology 71, no. 6 (April 2009): 910–19. http://dx.doi.org/10.1016/j.theriogenology.2008.10.022.
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Wicher, Victoria, Konrad Wicher, and Ronald F. Gruhn. "Increased Production of Antibodies to Spermatozoa and Seminal Fluid in Rabbits Used as Semen Donors." International Archives of Allergy and Immunology 83, no. 1 (1987): 104–8. http://dx.doi.org/10.1159/000234339.
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Copello, M., A. Perez, S. Marquez, and M. Sansinena. "202 SEX PRE-SELECTION IN RABBITS: AN ATTEMPT TO SKEW OFFSPRING SEX THROUGH PERCOLL AND SWIM-UP SPERM PREPARATION TECHNIQUES." Reproduction, Fertility and Development 24, no. 1 (2012): 213. http://dx.doi.org/10.1071/rdv24n1ab202.
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Sperm preparation techniques could have an effect on the birth ratio (male vs female offspring) through enrichment of either X- or Y-bearing sperm populations, although studies have been poorly controlled and results have been inconclusive. In the rabbit, producers may be interested in producing a majority of males (meat producing systems) or females (hybrid producing systems). The objective of this study was to evaluate the effect of modified Percoll and Swim-up protocols on the enrichment of the male and female birth ratios in rabbits. Four hybrid mature bucks of adequate body condition score and proven fertility were used. The same four bucks were used throughout the study (8 replicates). Ejaculates were collected using a warmed, solid, artificial vagina. Progressive motility was assessed at 200× magnification under brightfield microscopy on a heated (37°C) stage. Sperm concentration for each buck was determined using a Neubaur chamber and adjusted (350 × 106 total spermatozoa mL–1); they were then combined (1 mL/buck) to form a heterospermic sample (final heteropsermic sample volume = 4 mL). The heteropsermic sample was then divided into 3 sperm preparation treatments: (1) diluted in commercial tri-buffered extender followed by immediate AI (control); (2) modified Percoll centrifugation in a 90 to 45% density gradient (diluted in human tubal fluid medium); and (3) Swim-up (in human tubal fluid medium). In total, 125 females were inseminated. Does were treated with PMSG (20 UI/doe, 48 h before AI) and inseminated with 30 to 40 × 106 spermatozoa; ovulation was induced with 20 μg of gonadotropin-releasing hormone (GnRH)/doe immediately after AI. Sex of newborn kits was determined 35 days after birth by an experienced technician through external visualization of genitalia. Results were statistically analyzed using ANOVA and Tukey test (Infostat). The insemination of does with heterospermic ejaculates (control) resulted in a progeny distribution of 49 ± 2% males and 51 ± 2% females, which is in good agreement with the expected theoretical 50:50 ratio. Sex ratios from modified Percoll and Swim-up treatments deviated significantly from the control (P < 0.01). Percoll gradient resulted in a progeny distribution of 32 ± 2% males and 68 ± 2% females; Swim-up resulted in a 64 ± 2% male and a 36 ± 2% female distribution (P < 0.01), whereas prolificacy, perinatal mortality and birth weight were not affected by treatment (Table 1). Our results indicate sperm preparation techniques could be used to skew birth ratios towards male or female offspring in rabbits; Percoll preparation could be used to increase proportion of female kits whereas Swim-up could be used to increase the proportion of males. Further studies are needed to determine the underlying mechanisms of action. Table 1.Progeny distribution after Percoll or Swim-up sperm preparation in rabbits
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Kovács, M., G. Tornyos, Zs Matics, L. Kametler, V. Rajli, Zs Bodnár, M. Kulcsár, Gy Huszenicza, Zs Keresztes, and S. Cseh. "Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits." Animal 5, no. 10 (2011): 1563–69. http://dx.doi.org/10.1017/s1751731111000644.
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Chen, J., X. Yang, Y. Chen, and R. H. Foote. "In vitro fertilization in rabbits with spermatozoa capacitated in defined medium with or without lysophosphatidyl choline." Theriogenology 29, no. 1 (January 1988): 237. http://dx.doi.org/10.1016/0093-691x(88)90065-9.
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Vintoniv, O. A., and O. M. Havrysh. "THE REPRODUCTIVE ABILITY OF MALE RABBITS DEPENDING ON THE INFLUENCE OF PARATYPICAL AND GENOTYPICAL FACTORS." Animal Breeding and Genetics 64 (December 26, 2022): 147–53. http://dx.doi.org/10.31073/abg.64.13.
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The article provides information on the study of the level of reproductive ability of male rabbits of various breeds under conditions of industrial technology and retro-technology.
The purpose of the work was to analyze the features of the reproductive ability of rabbits of different breeds depending on the technology of keeping and the season of the year.
Materials and methods. The research was conducted on Poltava silver, California and New Zealand white rabbits (n = 27 rabbits) on the basis of two farms in the Cherkasy region – the experimental rabbit farm of the Cherkasy research station of bioresources (industrial technology) and the SG PE "Marchuk N. V." (retro technology).
When studying the reproductive capacity of male rabbits, sperm from breeder rabbits was collected every decade during the year, which, after dilution, was used to artificially inseminate female rabbits. At the same time, the semen was evaluated by the volume of the ejaculate, the motility of the spermatozoa and their concentration. The volume of the ejaculate was measured with the help of a graduated sperm receiver, the concentration and mobility of sperm – on the special equipment Sperm Vision (Minitube, Germany). Males were also assessed for the fertilizing ability of sperm.
The received research materials were processed by statistical methods using the "Statistica-6.1" software package and Excel (Microsoft Office 2007).
Research results. A study of the level of reproductive capacity of male rabbits of different breeds was carried out, depending on the technology of keeping and the influence of paratypic factors.
It was established that the average indicators of male ejaculate had the following values: ejaculate volume – 1.3–1.6 ml, sperm concentration – 330–390 million, sperm motility – 7.1–7.6 points, total number of sperm in ejaculate – 330–440 million.
The indicator of fertilizing capacity varied between 78–88%, the maximum value of this indicator was registered in rabbits of the NB breed, respectively, and the minimum in PS rabbits. A statistically significant interbreeding difference was established for all the investigated indicators of male sperm productivity. Males of the NB breed turned out to be the best in terms of overall fertilizing ability (88%). They outnumbered males of other groups in terms of ejaculate volume and sperm concentration. However, the mobility and general activity of sperm in the ejaculate were better in males of breed K.
Study of the influence of the technology of maintenance on the indicators of sperm productivity of the breeding stock of the farm "Marchuk N. V." make it possible to assert that there is no significant difference in the studied parameters. It was established that the animals that were kept according to retro technology had an average value of the ejaculate volume indicator 0.05 ml lower compared to the group of analogues that were kept indoors (p > 0.05). On average, the concentration of spermatozoa in the selected ejaculate was 347 million for males kept according to retro technology and 358 million for those kept according to industrial technology. The difference between these indicators turned out to be insignificant (p > 0.05), similar values were also recorded for the indicator of the total number of active sperm in the ejaculate. The sperm motility index in rabbits of all studied groups was 7.45–7.50 points, with a higher value recorded in the group of males kept in cages in the yard. Using one-factor variance analysis, a low influence of the specified factor on the studied indicators of male sperm was established (ηx2 = 0.01–0.04, p > 0.05).
Studies of the influence of the season of the year on sperm productivity indicators of male rabbits of the ChDSB experimental farm of the National Academy of Sciences show that in the vast majority of cases, the highest ejaculate volume indicator was recorded in winter, and the lowest in summer (Table 3). A tendency towards its gradual seasonal decrease from winter to summer and increase in autumn was revealed.
When determining the total number of active spermatozoa in both ejaculates, probable seasonal variability was also detected (a gradual decrease from winter to summer). This indicator was the highest in winter, and the lowest in summer (p < 0.001). In the spring, the investigated indicator was in the range of 294.7–439.2 million, and in the autumn period – 292.1–444.1 million.
The maximum concentration of sperm in the ejaculate was noted in winter (438.0 million/ml), and the minimum in summer (351.3 million/ml), the difference was 21.6% (p < 0.001). The regularity of a gradual seasonal decrease of this indicator from winter to summer and a slight increase in autumn was noted.
When evaluating sperm motility in the ejaculate, it was established that, in the vast majority of cases, this indicator was the highest in winter, and the lowest in the summer, however, the difference was not statistically significant (p > 0.05). The trend of a gradual seasonal decrease of this indicator from winter to summer and an increase in autumn was revealed.
Visible. Fertilizing ability of sperm in autumn was 6% better than in summer.
Using univariate variance analysis, a statistically significant influence of the season on sperm productivity indicators of the studied males was established according to the following parameters: the volume of the first ejaculate sperm – 6% (p < 0.05), the second ejaculate – 10% (p < 0.001); sperm concentration in the first ejaculate – 8% (p < 0.001); sperm motility in the first ejaculate – 11% (p < 0.001), in the second ejaculate – 3% (p < 0.05); the total number of active sperm in both ejaculates is 10–14% (p < 0.001); the number of calculated conditional sperm doses in the ejaculate is 13–14% (p < 0.001).
Conclusions.
The results of studying the indicators of sperm productivity of rabbits from two farms proved the absence of a probable influence of the breed on the indicators of sperm productivity of rabbits (ηx2 = 0.02–0.07, p > 0.05).
The study of the influence of the technology of keeping on the indicators of the sperm productivity of the breeders of the farm makes it possible to assert that there is no significant difference in the studied parameters.
The results of the univariate variance analysis proved the existence of a probable influence of the season on the indicators of sperm productivity of the studied males and on the vast majority of indicators of the reproductive capacity of female rabbits (4–3.14%).
A study of the fertilizing ability of male sperm by season showed that this indicator was the highest in spring – 88%, and the lowest – in summer (78%). 82% of females were fertilized in winter and 84% in autumn. there is a similar seasonal variability (gradual decrease from winter to summer and increase in autumn) when determining the total number of sperm in the ejaculate. This indicator was probably the highest in winter (438.6 million), and the lowest in summer (313.5 million), the difference was 28.52% (p < 0.001). A study of the fertilizing ability of male sperm by seasons showed that this indicator was the highest in spring, and the lowest in summer; the difference was 10%. In winter, females fertilized worse, the proportion of fertilized animals was 6% less than the canopy
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Kenfack, A., JK Chombong, F. Ngoula, NB Vemo, AMM Tsambou, GMZ Zeukeng, B. Mama, and J. Tchoumboué. "Effect of feeding cottonseed cake on male fertility in rabbit." Bangladesh Journal of Animal Science 44, no. 1 (April 29, 2015): 16–20. http://dx.doi.org/10.3329/bjas.v44i1.23123.
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In order to study the effects of cottonseed cake on the fertility of the male rabbit, 60 animals (40 bucks and 20 does) were used. Males were 3 months old at the beginning of the essay, and the females were multiparous. Males were divided into 4 identical groups. Four diets containing 0, 6, 12 and 18% of cottonseed cake were formulated and randomly allotted to different groups of males. All the females received the same diet (6%). The treatment lasted for 90 days. At the end of that period, 5 males were sacrificed per lot and the rest were allowed to mate each with an untreated female before being sacrificed in turn. No significant difference (p>0.05) was observed among rations for the volume of testis and weight of reproductive organs. Histological study of the testes revealed no abnormality. In the presence of an untreated female, hundred percent of rabbits realized mounting and ejaculated whichever the cottonseed cake level. Nevertheless, the volume of the ejaculate was significantly (p>0.05) low in rabbit fed 6% cottonseed cake (0.86 vs 1.66, 12%). The spermatozoa concentration in the cauda epididymis was higher in rabbit given cottonseed cake than in control, although not significantly (p>0.05). The adverse effects of the toxicant contained in cottonseed cake occurred at 18% inclusion. The result showed that it is not possible to generalize the upper limit of inclusion of cottonseed cake in the ration formulation.DOI: http://dx.doi.org/10.3329/bjas.v44i1.23123 Bang. J. Anim. Sci. 2014. 44 (1): 16-20
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de Curtis, I., G. Fumagalli, and N. Borgese. "Purification and characterization of two plasma membrane domains from ejaculated bull spermatozoa." Journal of Cell Biology 102, no. 5 (May 1, 1986): 1813–25. http://dx.doi.org/10.1083/jcb.102.5.1813.
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Plasma membranes were detached from ejaculated bull spermatozoa by a brief sonication in a moderately hypotonic medium, and the released plasma membranes were partially purified by differential centrifugation. The resulting fraction was enriched 8- and 15-fold in alkaline phosphatase and 5' nucleotidase activities, respectively, compared with the starting sonicated spermatozoa. This total plasma membrane fraction was separated into two distinct fractions by equilibrium density centrifugation on a continuous linear sucrose gradient. Two peaks of light scattering material were formed at densities of 1.117 and 1.148 g/ml. The denser peak contained most of the protein of the plasma membrane fraction, whereas nearly all the concanavalin A binding activity was found in the lighter peak. The two bands had distinctly different polypeptide compositions when analyzed by SDS PAGE. Polyclonal antibodies were raised in rabbits against a major integral membrane glycoprotein of each fraction (Mr of 92,000 in the light peak and 98,000 in the dense peak). The two antigens were detected on the surface of intact spermatozoa by indirect immunofluorescence microscopy. The 92-kD protein (present in the lighter band) was detected only on the plasma membrane of the acrosomal and anterior postacrosomal regions of the head. The 98-kD antigen, present in the heavier band, was localized to the surface of the postacrosomal region of the head, to the principal piece of the tail, and to the connecting piece between the head and tail. The exclusive localization of the 92-kD polypeptide to the surface of the anterior portion of the head was confirmed by immunoelectron microscopy. These data show that the two fractions isolated on the sucrose gradient originate from different regions of the sperm cell plasma membrane.
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Bodnár, K., Z. Szendró, E. Németh, C. Eiben, and I. Radnai. "Comparative evaluation of abnormal spermatozoa in Pannon White, New Zealand White and Angora rabbit semen (short communication)." Archives Animal Breeding 43, no. 5 (October 10, 2000): 507–12. http://dx.doi.org/10.5194/aab-43-507-2000.
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Abstract. The connection between sperm cell abnormalities and the breeds and seasons was studied. Semen was obtained from 20 Pannon White (PW), 20 New Zealand White (NZW) and 20 Angora (A) males monthly throughout a year. 697 ejaculates were evaluated. The average abnormal spermatozoa content of the ejaculates was 16.07 ± 1.77, 16.50 ± 0.2I and 27.34 ± 0.30 % respectively. The incidence of head and tail abnormalities was higher than that of other abnormalities in every group, but in A rabbits acrosome abnormalities were also at a significantly high level. The ratio of deformations was higher (P < 0.05) in all seasons in the A breed than in PW or NZW. Under Hungarian climate conditions the summer heat causes both primary and secondary degradation in semen quality. The effect of heat stress on spermatogenesis can be observed in the decreased semen quality in autumn.
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Fadl, Aya Mohamed, Khaled Hafez El-Shahat, and Elshymaa Ahmed Abdelnaby. "Influence of alpha linolenic acid on the motility, viability, antioxidant activity and fertility of frozen-thawed New Zealand white rabbit buck semen." World Rabbit Science 30, no. 3 (September 30, 2022): 219–26. http://dx.doi.org/10.4995/wrs.2022.17042.
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Freezing and thawing processes result in production and accumulation of high concentrations of reactive oxygen species that are detrimental to spermatozoal motility and fertility. Therefore, supplementation of exogenous source of antioxidants to freezing diluent is crucial. The aim of the present study was to investigate for the first time whether supplementation of semen diluent with alpha linolenic acid (ALA) can improve motility, viability, membrane integrity, antioxidant status and fertility of post-thaw rabbit spermatozoa. Semen was collected and pooled from fifteen New Zealand white rabbit bucks. Semen samples were diluted with a tris-citrate-glucose (TCG) extender supplemented with ALA (0, 50, 75 and 100 μmol). Then, extended rabbit semen was cooled at 5°C and cryopreserved in liquid nitrogen. After thawing, spermatozoal quality parameters (individual motility %, viability %, osmotic resistance %, and acrosome integrity %), antioxidant activity (SOD, CAT, and GSH activities), lipid peroxidation (malondialdehyde) and fertility (conception and kindling rates) were evaluated. Results revealed that supplementation of rabbit semen extender with 50 μmol ALA significantly (P<0.05) increased spermatozoal characteristics including motility (56.54%), viability (60.01%), acrosome status (72.66%) and membrane integrity (59.13%). The activity of semen antioxidant enzymes (SOD, CAT, and GSH) showed a significant improvement with a marked decrease in lipid peroxidation. Moreover, the conception (73.30%) and kindling (70.00%) rates were significantly (P<0.05) higher in does inseminated with thawed semen treated with 50 μmol ALA in comparison with other concentrations (0, 75 and 100 μmol). In summary, supplementation of rabbit semen extender with 50 μmol ALA improved motility, viability, membrane integrity, acrosome integrity, antioxidant enzymes activity and fertility of post-thaw rabbit spermatozoa. Our findings suggested that higher concentrations of ALA are detrimental to post-thaw characteristics of New Zealand white rabbit buck spermatozoa. To achieve better results, the semen freezing extender should be supplemented with ALA at lower concentrations, especially 50 μmol.
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Díaz Cano, Jesús V., María-José Argente, and María-Luz García. "Effect of Postbiotic Based on Lactic Acid Bacteria on Semen Quality and Health of Male Rabbits." Animals 11, no. 4 (April 3, 2021): 1007. http://dx.doi.org/10.3390/ani11041007.
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The aim of this study was to evaluate the effect of lactic acid bacteria-based postbiotic supplementation on semen characteristics and hematological and biochemical profiles in rabbits. A total of 28 males were randomly allocated into two groups. Males received a Control diet and Enriched diet supplemented with postbiotic for 15 weeks (4 weeks of adaptation period and 11 weeks of experimental period). Body weight, feed intake and semen characteristics were recorded weekly. Hematological profile was recorded at the beginning and end of the experiment and biochemical profile at 0, 5, 10 and 15 weeks. Bayesian methodology was used for the statistical analysis. Feed intake was higher in Control diet (125.2 g) than in the Enriched diet (118.6 g, p = 1.00). The percentages of abnormal spermatozoa were higher in Control diet than in Enriched diet (30% and 22%; p = 0.93) and the acrosome integrity percentage was lower (97% and 96%; p = 0.87). The hematological profile was within the range for healthy rabbits. The plasmatic level of alanine aminotransferase was higher in Control diet than Enriched diet at 5 and 10 weeks (p = 0.93 and p = 0.94, respectively) and alkaline phosphatase was similar in Control diet throughout the experiment, but decreased in Enriched diet (p = 0.97). No difference was found in kidney parameters (uric nitrogen and creatinine). Enriched diet showed higher total protein and globulin than Control diet (p = 0.99). Phosphorus was lower (p = 0.92) in Control diet than in Enriched diet. In conclusion, the addition of the postbiotic based on lactic acid bacteria seems to improve the quality of the semen and the liver profile in rabbits.
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Roychoudhury, Shubhadeep, Jaroslav Jedlicka, Vladimir Parkanyi, Jan Rafay, Lubomir Ondruska, Peter Massanyi, and Jozef Bulla. "Influence of a 50 Hz extra low frequency electromagnetic field on spermatozoa motility and fertilization rates in rabbits." Journal of Environmental Science and Health, Part A 44, no. 10 (August 3, 2009): 1041–47. http://dx.doi.org/10.1080/10934520902997029.
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Slavov, M., T. Diemer, I. Schroeder-Printzen, R. Henkel, K. Steger, M. Bergmann, and W. Weidner. "523 Testicular histology, chromatin condensation, and expression of nuclear proteins in spermatozoa and round spermatids in vasectomised rabbits." European Urology Supplements 3, no. 2 (February 2004): 133. http://dx.doi.org/10.1016/s1569-9056(04)90520-9.
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Adeyemi, Adenike Abiodun, Oyinofeoluwa Wuraola Ibrahim, Oluwabusayomi Omotoyosi Ajayi, and Samuel Tobi Ayeni. "Semen indices, growth response and sperm reserve of male rabbits fed zinc supplemented diets." World Rabbit Science 28, no. 4 (December 30, 2020): 199. http://dx.doi.org/10.4995/wrs.2020.13507.
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<p>This study was carried out to assess the effect of dietary levels of zinc on semen indices, growth parameters and testicular sperm reserve of heterogeneous stock of male rabbits. Twenty male rabbits aged 6 mo old were randomly allotted to four groups of five males each and fed diets containing 0, 50, 100 and 150 mg of zinc gluconate per kg diet, respectively, for eight weeks. Semen was collected from the males weekly using an artificial vagina and semen volume (mL), spermatozoa motility (%), sperm concentration (×10<sup>8</sup>/mL) and live sperm cells (%) were assessed. Seminal plasma was separated from the semen by centrifugation, and its zinc concentration was determined using atomic absorption spectrometry. The weights of the males were taken weekly, and the daily feed intake was recorded. At the end of the feeding trial, the rabbits were stunned and slaughtered; the testes and epididymis were carefully removed and homogenised to determine sperm reserves using standard procedure. The result showed that semen indices were not significantly influenced by the dietary levels of zinc gluconate. Seminal zinc concentration was significantly higher in males fed 100 and 150 mg of zinc per kg compared to those in the control group. A higher body weight gain (454±50.3 g), testes weight (6.23±0.25 g) and epididymis weight (1.63±0.59 g) was recorded in males fed diet supplemented with 50 mg of Zinc gluconate per kg. No significant effect of dietary zinc supplementation was recorded in testicular sperm reserve. Epididymal sperm reserve was significantly higher in males fed the diets including 100 and 150 mg of zinc gluconate per kg. In conclusion, dietary levels of zinc gluconate did not improve semen quality and growth indices but increased seminal zinc concentration, which could result in improved prostate health in the heterogeneous stock of male rabbits in the tropics.</p>
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Soler-García, Ángel A., Rangan Maitra, Vasantha Kumar, Tomoko Ise, Satoshi Nagata, Richard Beers, Tapan K. Bera, and Ira Pastan. "The PATE gene is expressed in the accessory tissues of the human male genital tract and encodes a secreted sperm-associated protein." Reproduction 129, no. 4 (April 2005): 515–24. http://dx.doi.org/10.1530/rep.1.00576.
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ThePATEgene is expressed in prostate and testis. To determine if PATE is expressed in other accessory tissues of the male genital tract, RT-PCR of the epididymis and seminal vesicle was performed. PATE mRNA was highly expressed in the epididymis and seminal vesicle.In situhybridization of the testis showed PATE mRNA is strongly expressed in the spermatogonia. ThePATEgene encodes a 14-kDa protein with a predicted signal sequence and a cleavage site between residues G21 and S22. To determine if PATE is a secreted protein, 293T cells were transfected with a pcDNA-PATE-myc-His plasmid and protein immunoprecipitated with anti-myc monoclonal antibody. Western blot analysis showed the presence of PATE-myc-His protein was in the medium and the cell lysate. Confocal microscopy demonstrated that PATE-myc-His protein is found in the endoplasmic reticulum. The polyclonal antibody SOL-1 was generated by immunization of rabbits with recombinant PATE protein expressed and purified fromEscherichia coli.Western blots were performed on extracts of prostate, testis, seminal vesicle and ejaculated spermatozoa, but PATE protein was only detected in the spermatozoa. Immunostaining of sperm smears revealed that PATE is located in a band-like pattern in the sperm head. Our data indicate that PATE is made by various sexual accessory tissues and secreted into the semen where it becomes associated with sperm, suggesting that PATE is a novel sperm-associated protein with a possible role in mammalian sperm maturation.
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Massányi, Peter, Martin Massányi, Roberto Madeddu, Robert Stawarz, and Norbert Lukáč. "Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs." Toxics 8, no. 4 (October 29, 2020): 94. http://dx.doi.org/10.3390/toxics8040094.
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Reproductive organs are essential not only for the life of an individual but also for the survival and development of the species. The response of reproductive organs to toxic substances differs from that of other target organs, and they may serve as an ideal “barometer” for the deleterious effects of environmental pollution on animal and human health. The incidence of infertility, cancers, and associated maladies has increased in the last fifty years or more, while various anthropogenic activities have released into the environment numerous toxic substances, including cadmium, lead, and mercury. Data from epidemiological studies suggested that environmental exposure to cadmium, lead, and mercury may have produced reproductive and developmental toxicity. The present review focused on experimental studies using rats, mice, avian, and rabbits to demonstrate unambiguously effects of cadmium, lead, or mercury on the structure and function of reproductive organs. In addition, relevant human studies are discussed. The experimental studies reviewed have indicated that the testis and ovary are particularly sensitive to cadmium, lead, and mercury because these organs are distinguished by an intense cellular activity, where vital processes of spermatogenesis, oogenesis, and folliculogenesis occur. In ovaries, manifestation of toxicity induced by cadmium, lead, or mercury included decreased follicular growth, occurrence of follicular atresia, degeneration of the corpus luteum, and alterations in cycle. In testes, toxic effects following exposure to cadmium, lead, or mercury included alterations of seminiferous tubules, testicular stroma, and decrease of spermatozoa count, motility and viability, and aberrant spermatozoa morphology.
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Easa, Fadila, Amal Hekal, and T. Seleem. "INJECTION EFFECT OF GIBBERELLIC AND/ OR BORIC ACID TO RABBITS ON RESPONSE OF SPERMATOZOA TO HYPO-OSMOTIC SWELLING TEST." Journal of Animal and Poultry Production 4, no. 10 (October 1, 2013): 571–81. http://dx.doi.org/10.21608/jappmu.2013.71540.
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El-Menoufy, A. A., A. A. Radwan, and M. M. El-Habbak. "Effect of Prostaglandin F2α and its Synthetic Analogue on Retention of Spermatozoa in the Reproductive Tract of Estrous Rabbits." Reproduction in Domestic Animals 20, no. 3 (September 1985): 200–205. http://dx.doi.org/10.1111/j.1439-0531.1985.tb00414.x.
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Ermayanti, N. G. A. M., N. M. R. Suarni, and I. G. A. M. Widhyastini. "Effect of Cod Liver Oil Supplementation in Feed on the Haematological Values and Concentration of Epididymis Spermatozoa of Local Rabbits." IOP Conference Series: Earth and Environmental Science 709, no. 1 (March 1, 2021): 012057. http://dx.doi.org/10.1088/1755-1315/709/1/012057.
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Fodor, Kinga, L. Zöldág, S. Gy Fekete, A. Bersényi, A. Gáspárdy, Emese Andrásofszky, Margit Kulcsár, F. Eszes, and M. Shani. "Influence of feeding intensity on the growth, body composition and sexual maturity of male New Zealand White rabbits." Acta Veterinaria Hungarica 51, no. 3 (July 1, 2003): 305–19. http://dx.doi.org/10.1556/avet.51.2003.3.6.
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An experiment was carried out with young male New Zealand White (NZW) rabbits to establish live body weight changes, body measurements, body composition and sexual maturity as a function of feeding intensity. Animals in Group 1 ('AL', n = 10) were fed ad libitum, while those in Group 2 ('RS', n = 10) received restricted feeding corresponding to 70% of the ad libitum level. The starting liveweights were practically the same (0.907 ± 0.146 and 0.911 ± 0.147 kg in Group AL and Group RS, respectively). The feeding trial lasted from 6 to 22 weeks of age. The average body weight was significantly higher in Group AL from 7 to 22 weeks of age. At 22 weeks of age the body weight of RS rabbits was 85.64% of the weight of AL animals (3.22 ± 0.52 kg and 3.76 ± 0.33 kg, respectively). Average body weights of RS males at 8, 9, 11, 19 and 21 weeks of age were similar to those of ad libitum fed (AL) animals at 7, 8, 10, 15 and 16 weeks of age, respectively. The growth of bucks fed restricted tended to be allometric. The most significant difference was found at 16 and 18 weeks of age, while the lowest difference occurred at 6, 12, 15 and 19 weeks of age. It can be stated that low-intensity feeding up to slaughtering weight causes backwardness in rear cannon length and this backwardness remains also after the 15th week, which is well over the optimal slaughtering age. Based on the present data, the 70% restricted feeding cannot be recommended either for the future breeding bucks or for broiler males reared for slaughter. To determine the major chemical components of the body, rabbits were euthanised. Original dry matter and crude fat content of the body significantly (P < 0.05) decreased under restricted feeding (41.42%; 32.48% and 16.73%; 7.35%) while the percentage of protein within the dry matter increased (49.6%; 65.0%) and fat decreased (40.17%; 22.1%) significantly. Libido unambiguously decreases as a consequence of feed deprivation. The most conspicuous difference was found in the level of blood testosterone. Although a few RS bucks produced semen but only much later than the rabbits fed ad libitum. On the other hand, there was no difference in the motility of spermatozoa and ejaculate volume in comparison with AL animals. There was no relationship between the body fat content and the reproductive status of bucks in the present trial.
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Solomom, I. P., E. F. Istifanus, and E. J. Idang. "Effect of graded dietary levels of Roselle (Hibiscus sabdariffa) calyx meal supplementation on sperm profile of mongrel rabbit bucks." Nigerian Journal of Animal Production 49, no. 3 (June 9, 2022): 34–45. http://dx.doi.org/10.51791/njap.v49i3.3529.
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Oxidative stress has been identified as one of the factors contributing to poor quality semen. Antioxidants however have been found useful in reducing oxidative stress thus protecting spermatozoa from reactive oxygen species (ROS)-induced production of abnormal spermatozoa and prevent deoxyribonucleic acid(DNA) fragmentation, thereby, improving semen quality. Roselle calyx has been identified as a rich source of antioxidants prominent among which is vitamin C (ascorbic acid). This experiment was conducted to evaluate the effect of graded dietary levels of Roselle calyx meal supplementation on sperm profile of mongrel rabbit bucks. Twenty clinically screened mongrel rabbit bucks were used for the study. The rabbits were managed intensively and were provided with feed, water and forages ad-libitum. Four experimental diets were formulated to contain dietary levels of Roselle calyx meal at 0.0% (control), 2.0%, 4.0% and 6.0% and coded as T1 , T2 , T3 and T4 respectively. The four treatment groups were assigned to four experimental diets in a completely randomized design (CRD) each replicated three times with two rabbits per replicate. Each replicate received an assigned diet for 56 days. Parameters measured in the were semen volume, semen colour motility, sperm concentration, live sperm percentage, abnormal sperm cells percentage, total sperm cells per ejaculate and reaction time. The semen volume (mL) recorded in this study were 0.47, 1.73, 0.57 and 0.53 for T (0.0% RCM), 1 T (2.0% RCM), T (4.0% RCM) and T (6.0% RCM,) respectively. Sperm motility was higher 2 3 4 in T (2.0% RCM) group with a value of 82.6% while the control group (0.0% RCM) recorded 2 the least value of 68.6%. Rabbit bucks in T (4.0% RCM) and T (6.0% RCM) had higher 3 4 sperm motility values of 78.3% and 81.6% respectively above those in control group. From the findings of this study Roselle calyx meal supplementation at 2.0% improved sperm characteristics of mongrel rabbit bucks.
Le stress oxydatif a été identifié comme l'un des facteurs contribuant au sperme de mauvaise qualité. Des antioxydants ont toutefois été utiles pour réduire le stress oxydatif protégeant ainsi les spermatozoïdes provenant de la production réactive d'espèces d'oxygène (REO) à la production d'une spermatozoïque anormale et prévenir la fragmentation de l'acide désoxyribonucléique (ADN), améliorant ainsi la qualité du sperme. RoselleCalyx a été identifiée comme une source riche d'antioxydants importante dont la vitamine C est la vitamine C (acide ascorbique). Cette expérience a été menée pour évaluer l'effet des niveaux diététiques gradués de la supplémentation de repas RoselleCalyx sur le profil de sperme des daims de lapin de Mongrel. Vingt daims de lapin mongrel cliniquement criblés ont été utilisés pour l'étude. Les lapins ont été gérés de manière intensive et ont été fournis avec des aliments pour animaux, de l'eau et des forages ad-libitum. Quatre régimes expérimentaux ont été formulés pour contenir des niveaux alimentaires de farine de CalyxRoselle à 0,0% (contrôle), de 2,0%, 4,0% et 6,0% et codé sous T , T , T et T respectivement. Les quatre 1 2 3 4 groupes de traitement ont été attribués à quatre régimes expérimentaux dans une conception entièrement randomisée (CER) répliquée chacune trois fois avec deux lapins par réplication. Chaque réplique a reçu une alimentation assignée pendant 56 jours. Les paramètres mesurés dans le volume de sperme, la motilité de la couleur du sperme, la concentration de sperme, le pourcentage de sperme en direct, pourcentage de spermatozoïdes anormaux, cellules de sperme total par éjaculation et temps de réaction. Le volume de sperme (ml) enregistré dans cette étude était de 0,47, 1,73, 0,57 et 0,53 pour T1 (0,0% de la MRC), T2 (2,0% de la MRC), T (RCM de 4.0%) et T (6,0% de la MRC) respectivement. La motilité du 3 4 sperme était plus élevée dans le groupe T (2.0% de la MRC) d'une valeur de 82,6% tandis 2 que le groupe témoin (0,0% de la MRC) a enregistré la moindre valeur de 68,6%. Les billes de lapin en T (RCM de 4,0%) et T (6,0% de la MRC) avaient des valeurs de motilité de 3 4 sperme supérieur de 78,3% et 81,6% respectivement au-dessus de ceux du groupe témoin. Des conclusions de cette étude RoselleCalyx Supplémentation de repas à 2,0% des caractéristiques de sperme améliorées de Daims de lapin de Mongrel.
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Klepko, A. V., Yu A. Kondratova, and I. M. Gudkov. "The role of rabbit seminal plasma natural antioxidants in the realization of spermatozoid activity after ionizing irradiation." Faktori eksperimental'noi evolucii organizmiv 26 (September 1, 2020): 132–38. http://dx.doi.org/10.7124/feeo.v26.1255.
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Aim. To investigate the effects of X-rays on the content of rabbit seminal plasma L-carnitine and α-tocopherol, as well as physiological parameters of spermatozoid in 10, 30 and 90 days after whole body irradiation of animals in the dose range of 0.1–2.0 Gy. Methods. White rabbits-males Soviet Shinshila were irradiated by X-rays. Physiological parameters of sperm were evaluated by light microscopy, and the content of natural antioxidants was determined using liquid chromatograph “Agilent 1200”. Results. It was shown that low doses of ionizing radiation (0.1 Gy and 0.5 Gy) in 10 days after irradiation lead to the increase of the L-carnitine level in seminal plasma, whereas at 1.0 Gy and 2.0 Gy its concentration decreases. In additional, there is a decrease in the seminal plasma α-tocopherol content with a minimum of 2.0 Gy, although a dose of 0.1 Gy dose not cause changes in the index. The L-carnitine level is no longer different from the control for a dose 0.1 Gy in one month after rabbit irradiation, while the α-tocopherol concentration continues to decrease. Conclusions. The increasing of L-carnitine level in the rabbit’s seminal plasma at 0.1 Gy and 0.5 Gy contributes to increased motility and a radio-stimulating effect on sperm. Radiation-induced depletion of the α-tocopherol pool in semen disappears at a later period.
Keywords: ionizing radiation, rabbits, seminal plasma, natural antioxidants