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1

Yu, Longtao, Xinxin Shi, Xujiang He, Zhijiang Zeng, Weiyu Yan, and Xiaobo Wu. "High-Quality Queens Produce High-Quality Offspring Queens." Insects 13, no. 5 (May 23, 2022): 486. http://dx.doi.org/10.3390/insects13050486.

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Honey bees, rather than rear queens with eggs and larvae from worker cells, prefer to rear new queens with eggs form queen cells, if available. This may be a result of long-term evolutionary process for honey bee colonies. However, the exact mechanism of this phenomenon is unclear. In this study, queens were reared with eggs from queen cells (F1-QE), eggs from worker cells (F1-WE), and two-day-old larvae from worker cells (F1-2L). Physiological indexes and the expression of the development-related genes ((Hexamerin (Hex110, Hex70b), Transferrin (Trf), and Vitellogenin (Vg)) of reared F1 generation queens were measured and compared. Furthermore, F2 generation queens were reared with one-day-old larvae from F1 queens, and the weight and ovariole count of reared F2 generation daughter queens were examined. Meanwhile, the expression of the development- and reproduction-related genes (Hex110, Hex70b, Trf, Vg, and Juvenile Hormone (Jh)) and immune detoxication-related genes (Hymenoptaecin, Abeacin, and CytP450) of reared F2 queens were further explored. We found that the F1-QE queens had the highest physiological indexes and higher Hex110 and Trf expression levels, while no significant difference was found in the expression of Hex70b and Vg among the three groups of F1 queens. In addition, the reared queens of F2-QE had the highest quality, with the highest development, reproduction, immune-detoxication genes’ expression levels. Our results revealed that the quality of reared offspring queens from high-quality mother queens was also high. These findings inform methods for rearing high-quality queens and highlight that a high-quality queen is essential for offspring colony growth and survival.
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2

Wu, Xiaobo, Linbin Zhou, Chuibin Zou, and Zhijiang Zeng. "Effects of Queen Cell Size and Caging Days of Mother Queen on Rearing Young Honey Bee Queens Apis mellifera L." Journal of Apicultural Science 62, no. 2 (December 1, 2018): 215–22. http://dx.doi.org/10.2478/jas-2018-0025.

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Abstract This study aims to investigate the effect of queen cell size (9.4 mm, 9.6 mm, 9.8 mm and 10.0 mm) and mother queen caged time (0 day, 2 days and 4 days) on rearing young queens without grafting larvae. The birth weight, ovarian tubes, thorax length and width were significantly increased with the increasing diameter of queen cell size. The expression level of Vitellogenin (Vg) in young queen ovaries was also up-regulated with the increased queen cell size diameter. These results indicate that the queen cell size can strongly affect the rearing queen quality and reproductive ability. Moreover, the weight, length and width of laying eggs rose with the mother queen caging time, and young queens reared with the hatched larvae from these eggs were also increased in terms of birth weight, ovarian tubes, thorax length and width. Furthermore, the expression level of Vg in reared queen ovaries was also up-regulated with the caged time. These results reveal that the caged time of queens could significantly influence egg size and their relative queen quality.
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Lee, Kathleen V., Michael Goblirsch, Erin McDermott, David R. Tarpy, and Marla Spivak. "Is the Brood Pattern within a Honey Bee Colony a Reliable Indicator of Queen Quality?" Insects 10, no. 1 (January 8, 2019): 12. http://dx.doi.org/10.3390/insects10010012.

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Failure of the queen is often identified as a leading cause of honey bee colony mortality. However, the factors that can contribute to “queen failure” are poorly defined and often misunderstood. We studied one specific sign attributed to queen failure: poor brood pattern. In 2016 and 2017, we identified pairs of colonies with “good” and “poor” brood patterns in commercial beekeeping operations and used standard metrics to assess queen and colony health. We found no queen quality measures reliably associated with poor-brood colonies. In the second year (2017), we exchanged queens between colony pairs (n = 21): a queen from a poor-brood colony was introduced into a good-brood colony and vice versa. We observed that brood patterns of queens originally from poor-brood colonies significantly improved after placement into a good-brood colony after 21 days, suggesting factors other than the queen contributed to brood pattern. Our study challenges the notion that brood pattern alone is sufficient to judge queen quality. Our results emphasize the challenges in determining the root source for problems related to the queen when assessing honey bee colony health.
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4

Güneşdoğdu, Mustafa, and Ahmet Şekeroğlu. "Kraliçe (Ana) Arı Kalitesini Etkileyen Faktörler." Turkish Journal of Agriculture - Food Science and Technology 8, sp1 (December 14, 2020): 197–202. http://dx.doi.org/10.24925/turjaf.v8isp1.197-202.4095.

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The basic rule in honeybee (Apis mellifera L.) rearing is to work with qualified queens. Quality queen means strong and healthy colonies. Honey bee colonies are a social community, each managed by a single queen bee. The queen bee is the only female that produces eggs in the colony under normal conditions, and the quality and healthy queen is important for the continuity of the colony. Many factors affect the quality of the queen bee, such as the rearing colony, the age of the transferred larva and the transfer medium, the season, cell emergence weight, spermatheca charged, the characteristics of the starter and finisher hives. In this paper, the issues to be considered in raising a quality queen are emphasized.
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5

Mahbobi, Ali, Mohammadbagher Farshineh-Adl, Jerzy Woyke, and Saeed Abbasi. "Effects of the Age of Grafted Larvae and the Effects of Supplemental Feeding on Some Morphological Characteristics of Iranian Queen Honey Bees (Apis mellifera meda Skorikov, 1929)." Journal of Apicultural Science 56, no. 1 (June 1, 2012): 93–98. http://dx.doi.org/10.2478/v10289-012-0010-1.

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Effects of the Age of Grafted Larvae and the Effects of Supplemental Feeding on Some Morphological Characteristics of Iranian Queen Honey Bees (Apis mellifera medaSkorikov, 1929)The research was conducted at the apiary of the Faculty of Agriculture, Zanjan University in Zanjan, Iran. Queens were reared in 24Apis mellifera medahoney bee rearing colonies. The colonies were assigned to 4 grafting larvae age groups; 1 day old larvae, 2 day old larvae, and 3 day old larvae, and the last group reared emergency queen cells. The groups were divided into the 2 feeding groups: fed additionally and no fed. The effects of the age of the grafted larvae and the effects of supplemental feeding on 9 morphological characteristics of queens were measured. The results showed that the age of the larvae significantly affected the morphological characteristics of reared queens, and thus, their quality. Queens reared from 1 day old larvae were of the highest quality. These queens were significantly heavier (158.83 mg) and had significantly larger spermatheca (0.99 mm3) than queens reared from larvae 2 and 3 days old. Queens from emergency queen cells were of lower quality than queens reared from 1 day old larvae. However, queens from emergency queen cells were of higher quality than queens reared from 3 day old larvae. The supplemental feeding significantly increased most morphological characteristics of the reared queens. The different ages of the larvae did not significantly affect the wing length nor did supplemental feeding affect the wing length.
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6

Gregorc, Aleš, and Maja I. Smodiš Škerl. "Characteristics of Honey Bee (Apis Mellifera Carnica, Pollman 1879) Queens Reared in Slovenian Commercial Breeding Stations." Journal of Apicultural Science 59, no. 2 (December 1, 2015): 5–12. http://dx.doi.org/10.1515/jas-2015-0016.

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AbstractIn this three-year-trial study, we examined the quality of mated queens based on morphological and physiology traits. At each location, sister queen bees were reared each year from one Apis mellifera carnica breeder queen. Queens were also reared and mated in different locations. Altogether, we sampled and analysed 324 queens from 27 apiaries in 2006, 288 queens from 24 apiaries in 2008, and 276 queens from 23 apiaries in 2010. Nine queens from each apiary were sampled and dissected for morphological analyses and Nosema ceranae (N. ceranae) spores, if present, were quantified. Three queens from each apiary were prepared and tested for four viruses: acute bee paralysis virus (ABPV), black queen cell virus (BQCV), deformed wing virus (DWV), and sacbrood virus (SBV). The highest average queen weight of 209.49 ± 9.82 mg was detected in 2008. The highest average ovary weight of 78.67 ± 11.86 mg was detected in 2010, and the highest number of ovarioles was 161.59 ± 8.70 in 2006. The average number of spermatozoa in queens ranged from 3.30 x 106 in 2006 to 5.23 x 106 in 2010. Nosema ceranae spores were found in queens sampled in 2008 and 2010. Viruses were discovered sporadically during the queen testing periods from 2006 - 2010. This study importantly demonstrates that queens from rearing stations require regular evaluation for morphological and physiological changes as well as for infection from harmful pathogens. These results could also be used in establishing relevant commercial standards for rearing quality queens.
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7

Gregorc, Aleš, and Tamás Bakonyi. "Viral infections in queen bees (Apis mellifera carnica) from rearing apiaries." Acta Veterinaria Brno 81, no. 1 (2012): 15–19. http://dx.doi.org/10.2754/avb201281010015.

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Viral infection could have an impact on the success of queen rearing and a potential effect on reduced queen quality. Newly mated honey bee (Apis mellifera carnica) queens were collected from mating nuclei in queen rearing operations in Slovenia. Altogether, 81 queens were sampled from 27 rearing apiaries in 2006 and 72 queens from 24 apiaries in 2008. Queens were analysed for the presence of four viruses: acute bee paralysis virus (ABPV), black queen cell virus (BQCV), sacbrood virus (SBV) and deformed wing virus (DWV) by using reverse transcription polymerase chain reaction (RT-PCR). In 2006, 12%, 9% and 1% prevalence was found for ABPV, DWV and SBV, respectively; BQCV was not detected. Two years later, DWV, BQCV, SBV and ABPV were detected in 58%, 24%, 11% and 10% bee queens, respectively. In 2006, fourteen out of twenty-seven apaiaries were virus free, whereas in 2008 only three out of twenty-four apiaries were virus free. This is the first evidence of virus infection occurring in newly mated queens from mating nuclei in rearing apiaries. The possible impacts of queen rearing technology and epidemiological influences on virus infection are discussed in this study.
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8

Wyborn, M. H., M. L. Winston, and P. H. Laflamme. "MASS STORAGE OF HONEY BEE (HYMENOPTERA: APIDAE) QUEENS DURING THE WINTER." Canadian Entomologist 125, no. 1 (February 1993): 113–28. http://dx.doi.org/10.4039/ent125113-1.

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AbstractMass storage of honey bee (Apis mellifera L.) queens over the winter was investigated in colony banks, with each queen held in her own cage within a colony. The major treatments included: (I) a single queen wintered in a small nucleus colony (control); and colony banks with 24 or 48 queens, each held individually in (II) screen cages that prevented workers from entering the cage, but allowed access for queen tending, (III) queen-excluder cages (queen-excluder material has openings of about 55 mm that prevent the larger queen but not the smaller workers from passing through the material), or (IV) screen cages until January and subsequent transfer to mini-nuclei until late March. Queens held in excluder cages showed poor survival in all 3 years of testing, and this system was not viable for commercial use; survival for any 1 year, or any excluder treatment, was never greater than 25%. In contrast, a 2-year average of 60% queen survival was found for queens that were stored in individual screened wooden cages within queenless colony banks. We found no differences in survival of banked queens that were moved between colonies monthly and those that remained in the same colony for 6 months. The success of these systems required the (a) preparation of colony banks that contained large numbers of adult workers produced by maintaining colonies with two queens during the previous summer, (b) removal of laying queen(s) during the storage period, (c) feeding of colonies well, and (d) insulation of colonies in groups of four, to preserve heat and reduce worker clustering in the winter. Surviving queens from winter storage systems were virtually identical in quality and colony performance to control queens the subsequent season. The annual profit for a commercial beekeeper who does his/her own labour for storage and hires workers for queen production was calculated as $16,625 when 4800 queens are stored over the winter, and sold for $10 each in the spring. Thus, mass queen storage using our successful systems is both biologically and economically viable.
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9

He, Xu Jiang, Hao Wei, Wu Jun Jiang, Yi Bo Liu, Xiao Bo Wu, and Zhijiang Zeng. "Honeybee (Apis mellifera) Maternal Effect Causes Alternation of DNA Methylation Regulating Queen Development." Sociobiology 68, no. 1 (March 30, 2021): 5935. http://dx.doi.org/10.13102/sociobiology.v68i1.5935.

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Queen-worker caste dimorphism is a typical trait for honeybees (Apis mellifera). We previously showed a maternal effect on caste differentiation and queen development, where queens emerged from queen-cell eggs (QE) had higher quality than queens developed from worker cell eggs (WE). In this study, newly-emerged queens were reared from QE, WE, and 2-day worker larvae (2L). The thorax size and DNA methylation levels of queens were measured. We found that queens emerging from QE had significantly larger thorax length and width than WE and 2L. Epigenetic analysis showed that QE/2L comparison had the most different methylated genes (DMGs, 612) followed by WE/2L (473), and QE/WE (371). Interestingly, a great number of DMGs (42) were in genes belonging to mTOR, MAPK, Wnt, Notch, Hedgehog, FoxO, and Hippo signaling pathways that are involved in regulating caste differentiation, reproduction and longevity. This study proved that honeybee maternal effect causes epigenetic alteration regulating caste differentiation and queen development.
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10

McAfee, Alison, David R. Tarpy, and Leonard J. Foster. "Queen honey bees exhibit variable resilience to temperature stress." PLOS ONE 16, no. 8 (August 11, 2021): e0255381. http://dx.doi.org/10.1371/journal.pone.0255381.

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Extreme temperature exposure can reduce stored sperm viability within queen honey bees; however, little is known about how thermal stress may directly impact queen performance or other maternal quality metrics. Here, in a blind field trial, we recorded laying pattern, queen mass, and average callow worker mass before and after exposing queens to a cold temperature (4°C, 2 h), hot temperature (42°C, 2 h), and hive temperature (33°C, control). We measured sperm viability at experiment termination, and investigated potential vertical effects of maternal temperature stress on embryos using proteomics. We found that cold stress, but not heat stress, reduced stored sperm viability; however, we found no significant effect of temperature stress on any other recorded metrics (queen mass, average callow worker mass, laying patterns, the egg proteome, and queen spermathecal fluid proteome). Previously determined candidate heat and cold stress biomarkers were not differentially expressed in stressed queens, indicating that these markers only have short-term post-stress diagnostic utility. Combined with variable sperm viability responses to temperature stress reported in different studies, these data also suggest that there is substantial variation in temperature tolerance, with respect to impacts on fertility, amongst queens. Future research should aim to quantify the variation and heritability of temperature tolerance, particularly heat, in different populations of queens in an effort to promote queen resilience.
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11

Brutscher, Laura M., Boris Baer, and Elina L. Niño. "Putative Drone Copulation Factors Regulating Honey Bee (Apis mellifera) Queen Reproduction and Health: A Review." Insects 10, no. 1 (January 8, 2019): 8. http://dx.doi.org/10.3390/insects10010008.

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Honey bees are major pollinators of agricultural and non-agricultural landscapes. In recent years, honey bee colonies have exhibited high annual losses and commercial beekeepers frequently report poor queen quality and queen failure as the primary causes. Honey bee colonies are highly vulnerable to compromised queen fertility, as each hive is headed by one reproductive queen. Queens mate with multiple drones (male bees) during a single mating period early in life in which they obtain enough spermatozoa to fertilize their eggs for the rest of their reproductive life span. The process of mating initiates numerous behavioral, physiological, and molecular changes that shape the fertility of the queen and her influence on the colony. For example, receipt of drone semen can modulate queen ovary activation, pheromone production, and subsequent worker retinue behavior. In addition, seminal fluid is a major component of semen that is primarily derived from drone accessory glands. It also contains a complex mixture of proteins such as proteases, antioxidants, and antimicrobial proteins. Seminal fluid proteins are essential for inducing post-mating changes in other insects such as Drosophila and thus they may also impact honey bee queen fertility and health. However, the specific molecules in semen and seminal fluid that initiate post-mating changes in queens are still unidentified. Herein, we summarize the mating biology of honey bees, the changes queens undergo during and after copulation, and the role of drone semen and seminal fluid in post-mating changes in queens. We then review the effects of seminal fluid proteins in insect reproduction and potential roles for honey bee drone seminal fluid proteins in queen reproduction and health. We finish by proposing future avenues of research. Further elucidating the role of drone fertility in queen reproductive health may contribute towards reducing colony losses and advancing honey bee stock development.
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12

Okuyan, Samet, and Paul Cross. "The Effects of Co-Enzyme Q10 and Caffeine on Morphometric Characteristics of Queen Honey Bees." Turkish Journal of Agriculture - Food Science and Technology 9, no. 2 (February 23, 2021): 321–25. http://dx.doi.org/10.24925/turjaf.v9i2.321-325.3903.

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Honey bee queen quality is a critical factor of colony performance. Indications of such qualities can manifest themselves through morphological traits such as wet weight and thorax width. Improving such characteristics is driven in part by nutritional provision in queen-cell-builder hives. We investigated the potential to improve queen quality by adding coenzyme Q10 (endogenous antioxidant) and caffeine (central nervous system stimulator) to feeder syrup in queen-cell-builder colonies for 15 and 20 days prior to grafting, two sets of queens were reared. We recorded subsequent wet weight, body length, head width and length, thorax and wing width and length, and spermathecae diameter. The queen-cell acceptance rate was not affected by either treatment or graft period. Coenzyme Q10 increased wet weight, body and wing length in the first graft, and thorax width, wing length and spermathecae diameter in the second graft. The caffeine treatment increased head and thorax length in first graft and thorax width in the second. A mix of the two substances (coenzyme Q10 and caffeine) increased head width in the first graft and spermathecae diameter in the second graft. This study suggests that the application of coenzyme Q10 to cell-builder colonies at least 15 days prior to grafting can increase reared wet weight (the most significant quality indicator) and thorax width of queen bees.
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Mukenov, S. K. "The influence of feed additives on the acceptance and quality of queen bees." Kormlenie sel'skohozjajstvennyh zhivotnyh i kormoproizvodstvo (Feeding of agricultural animals and feed production), no. 7 (July 1, 2021): 66–71. http://dx.doi.org/10.33920/sel-05-2107-07.

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Currently, many scientists have proven the positive influence of feed additives on the productivity of different types of farm animals including bees since feed additives have a positive effect on the honey productivity of bee colonies and the quality of queen bees. The purpose of our work was to identify the effect of probiotics on the background of the dietary supplement Eracond on the acceptance of larvae and the weight of infertile queens. The scientific and economic experiment has been carried out on bees of Bashkir breed in an apiary located in the village of Nekrasovka in the Kartalinsky district in the Chelyabinsk region. Three groups of queen-rearing colony have been formed three in each. In the 2nd and 3rd experimental groups against the background of dietary supplement Erakond (50 mg/ kg) have been given: in the 2nd experimental group sugar syrup with Lactobacterin three doses per family, in the 3rd experimental group sugar syrup with Vetom 1,1 at the dose of 50 mg/kg. As a result, all the additives in the 2nd and 3rd experimental groups gave a positive result. The greatest effect on all indicators has been obtained with the combined use of 50 % sugar syrup with the addition of Eracond phytopreparation (50 mg/kg) and Vetom 1,1 probiotic (50 mg/kg). Feeding these drugs to queen rearing colonies contributed to the increase in the acceptance of larvae by 23,3 %, compared with the control and by 7,8 % compared with the 2nd experimental group, also had the positive influence on the weight of infertile queens by 11,4 % compared with the control group. The use of Vetom and Eracond contributed to the increase in the egg production of queens by 64,2, 54,9 and 57,0 % compared with the control for three accounting periods as well as the increase in the honey collection by 33,83 % or 4,5 kg.
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14

Hatjina, Fani. "Greek Honey Bee Queen Quality Certification." Bee World 89, no. 1 (January 2012): 18–20. http://dx.doi.org/10.1080/0005772x.2012.11417453.

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15

Goras, Georgios, Chrysoula Tananaki, Sofia Gounari, Elissavet Lazaridou, Dimitrios Kanelis, Vasileios Liolios, Emmanouel Karazafiris, and Andreas Thrasyvoulou. "Rearing Drones in Queen Cells of Apis mellifera Honey Bees." Journal of Apicultural Science 60, no. 2 (December 1, 2016): 119–28. http://dx.doi.org/10.1515/jas-2016-0033.

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Abstract We investigated the rearing of drone larvae grafted in queen cells. From the 1200 drone larvae that were grafted during spring and autumn, 875 were accepted (72.9%) and reared as queens. Drone larvae in false queen cells received royal jelly of the same composition and of the same amounts as queen larvae. Workers capped the queen cells as if they were drones, 9-10 days after the egg laying. Out of 60 accepted false queen cells, 21 (35%) were capped. The shape of false queen cells with drone larvae is unusually long with a characteristically elongate tip which is probably due to the falling of larvae. Bees start the destruction of the cells when the larvae were 3 days old and maximised it before and after capping. Protecting false queen cells in the colony by wrapping, reversing them upside down, or placing in a horizontal position, did not help. The only adult drones that emerged from the false queen cells were those protected in an incubator and in push-in cages. Adult drones from false queen cells had smaller wings, legs, and proboscis than regular drones. The results of this study verify previous reports that the bees do not recognise the different sex of the larvae at least at the early stage of larval development. The late destruction of false queen cells, the similarity in quality and quantity of the produced royal jelly, and the bigger drone cells, allow for the use of drone larvae in cups for the production of royal jelly.
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16

Yi, Yao, Yi Bo Liu, Andrew B. Barron, and Zhi Jiang Zeng. "Transcriptomic, Morphological, and Developmental Comparison of Adult Honey Bee Queens (Apis mellifera) Reared From Eggs or Worker Larvae of Differing Ages." Journal of Economic Entomology 113, no. 6 (August 31, 2020): 2581–87. http://dx.doi.org/10.1093/jee/toaa188.

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Abstract Queens and workers are very distinct phenotypes that develop from the same genome. Larvae from worker cells up to 3.5 d old can be transferred to larger queen cells and will subsequently be reared as queens and develop into functional queens. This has become a very popular queen rearing practice in contemporary apiculture. Here we used RNA-Seq to study the consequences of rearing queens from transplanted worker larvae on the transcriptome of the adult queens. We found that queens reared from transferred older larvae developed slower, weighted less, and had fewer ovarioles than queens reared from transferred eggs, indicating queens were cryptically intercaste. RNA-Seq analysis revealed differentially expressed genes between queens reared from transferred larvae compared with queens reared from transferred eggs: the older the larvae transferred, the greater the number of differentially expressed genes. Many of the differentially expressed genes had functions related to reproduction, longevity, immunity, or metabolism, suggesting that the health and long-term viability of queens was compromised. Our finds verify the previous studies that adult queens reared from older transferred larvae were of lower quality than queens reared from transferred eggs or younger larvae.
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Slater, Garett P., George D. Yocum, and Julia H. Bowsher. "Diet quantity influences caste determination in honeybees ( Apis mellifera )." Proceedings of the Royal Society B: Biological Sciences 287, no. 1927 (May 27, 2020): 20200614. http://dx.doi.org/10.1098/rspb.2020.0614.

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In species that care for their young, provisioning has profound effects on offspring fitness. Provisioning is important in honeybees because nutritional cues determine whether a female becomes a reproductive queen or sterile worker. A qualitative difference between the larval diets of queens and workers is thought to drive this divergence; however, no single compound seems to be responsible. Diet quantity may have a role during honeybee caste determination yet has never been formally studied. Our goal was to determine the relative contributions of diet quantity and quality to queen development. Larvae were reared in vitro on nine diets varying in the amount of royal jelly and sugars, which were fed to larvae in eight different quantities. For the middle diet, an ad libitum quantity treatment was included. Once adults eclosed, the queenliness was determined using principal component analysis on seven morphological measurements. We found that larvae fed an ad libitum quantity of diet were indistinguishable from commercially reared queens, and that queenliness was independent of the proportion of protein and carbohydrate in the diet. Neither protein nor carbohydrate content had a significant influence on the first principle component 1 (PC1), which explained 64.4% of the difference between queens and workers. Instead, the total quantity of diet explained a significant amount of the variation in PC1. Large amounts of diet in the final instar were capable of inducing queen traits, contrary to the received wisdom that queen determination can only occur in the third instar. These results indicate that total diet quantity fed to larvae may regulate the difference between queen and worker castes in honeybees.
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Rousseau, Andrée, Valérie Fournier, and Pierre Giovenazzo. "Apis mellifera (Hymenoptera: Apidae) drone sperm quality in relation to age, genetic line, and time of breeding." Canadian Entomologist 147, no. 6 (April 24, 2015): 702–11. http://dx.doi.org/10.4039/tce.2015.12.

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AbstractA honey bee (Apis mellifera Linnaeus; Hymenoptera: Apidae) queen’s life expectancy is strongly dependent on the number of sperm she obtains by mating with drones during nuptial flights. Unexplained replacement of queens by the colony and young queens showing sperm depletions have been reported in North America, and reduced drone fertility has been a suspected cause. The aim of this study was to evaluate drone reproductive qualities during the queen-rearing season, from May to August. Drones from two different genetic lines were reared six times during the 2012 beekeeping season at our research centre in Québec (Canada). Semen volume as well as sperm number and viability were assessed at the ages of 14, 21, and 35 days. Results showed (1) a greater proportion of older drones with semen at the tip of the genitalia after eversion; (2) an influence of rearing date on semen production; and (3) no influence of drone genetic line, age or time of breeding on sperm viability. These results highlight the necessity of better understanding drone rearing and how it can be improved to ensure optimum honey-bee queen mating.
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Tarpy, David R., Shanti Hatch, and David J. C. Fletcher. "The influence of queen age and quality during queen replacement in honeybee colonies." Animal Behaviour 59, no. 1 (January 2000): 97–101. http://dx.doi.org/10.1006/anbe.1999.1311.

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20

Walsh, Elizabeth, and Juliana Rangel. "Local Honey Bee Queen Production and Quality." Bee World 93, no. 2 (October 24, 2016): 30–32. http://dx.doi.org/10.1080/0005772x.2016.1211501.

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21

Liebig, Jürgen, Thibaud Monnin, and Stefano Turillazzi. "Direct assessment of queen quality and lack of worker suppression in a paper wasp." Proceedings of the Royal Society B: Biological Sciences 272, no. 1570 (June 15, 2005): 1339–44. http://dx.doi.org/10.1098/rspb.2005.3073.

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Assessing a conspecific's potential is often crucial to increase one's fitness, e.g. in female choice, contests with rivals or reproductive conflicts in animal societies. In the latter, helpers benefit from accurately assessing the fertility of the breeder as an indication of inclusive fitness. There is evidence that this can be achieved using chemical correlates of reproductive activity. Here, we show that queen quality can be assessed by directly monitoring her reproductive output. In the paper wasp Polistes dominulus , we mimicked a decrease in queen fertility by regularly removing brood. This triggered ovarian development and egg-laying by many workers, which strongly suggests that brood abundance is a reliable cue of queen quality. Brood abundance can be monitored when workers perform regular brood care in small size societies where each brood element is kept in a separate cell. Our results also show that although the queen was not manipulated, and thus remained healthy and fully fertile, she did not control worker egg-laying. Nevertheless, when workers laid eggs, the queen secured a near reproductive monopoly by selectively destroying these eggs, a mechanism known as ‘queen policing’. By contrast, workers destroyed comparatively few queen-laid eggs, but did destroy each other's eggs.
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22

Hensler, K., T. Waschulzik, S. P. Mönig, K. Maruyama, A. H. Hölscher, and E. Bollschweiler. "Quality-assured Efficient Engineering of Feedforward Neural Networks (QUEEN)." Methods of Information in Medicine 44, no. 05 (2005): 647–54. http://dx.doi.org/10.1055/s-0038-1634021.

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Summary Objectives: Lymph node metastasis (LNM) is an important prognostic indicator in patients with gastric carcinoma. However, the methods that have been established for preoperative diagnosis of LNM show insufficient accuracy. Methods: This study describes the use of the QUality Assured Efficient Engineering of Feedforward Neural Networks with Supervised Learning (QUEEN) technique to attempt optimization of the preoperative diagnosis of lymph node metastasis in patients with gastric carcinoma. The results were compared with the Maruyama Diagnostic System (MDS) for preoperative prediction of LNM, established at the National Cancer Center in Tokyo. Results: QUEEN is able to extract predictive variables from a case-based database. The combination of a development method, a special type of neural network and the corresponding encoding yielded an accuracy of 72.73%, which is notably higher than that of the MDS. Our system produced a nearly ten per cent higher sensitivity and around eighteen per cent higher specificity than MDS. Conclusion: Our results show that QUEEN is a reasonable method for the development of ANNs. We used the QUEEN system for prediction of LNM in gastric cancer. This system may allow more meaningful preoperative planning by gastric surgeons.
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Holman, Luke, Charlotte G. Jørgensen, John Nielsen, and Patrizia d'Ettorre. "Identification of an ant queen pheromone regulating worker sterility." Proceedings of the Royal Society B: Biological Sciences 277, no. 1701 (June 30, 2010): 3793–800. http://dx.doi.org/10.1098/rspb.2010.0984.

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The selective forces that shape and maintain eusocial societies are an enduring puzzle in evolutionary biology. Ordinarily sterile workers can usually reproduce given the right conditions, so the factors regulating reproductive division of labour may provide insight into why eusociality has persisted over evolutionary time. Queen-produced pheromones that affect worker reproduction have been implicated in diverse taxa, including ants, termites, wasps and possibly mole rats, but to date have only been definitively identified in the honeybee. Using the black garden ant Lasius niger , we isolate the first sterility-regulating ant queen pheromone. The pheromone is a cuticular hydrocarbon that comprises the majority of the chemical profile of queens and their eggs, and also affects worker behaviour, by reducing aggression towards objects bearing the pheromone. We further show that the pheromone elicits a strong response in worker antennae and that its production by queens is selectively reduced following an immune challenge. These results suggest that the pheromone has a central role in colony organization and support the hypothesis that worker sterility represents altruistic self-restraint in response to an honest quality signal.
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Sheremeta, V. I., I. V. Vedmid, and V. G. Kaplunenko. "THE QUALITY OF QUEEN BEES BY FEEDING THE QUEEN BEE FAMILIES WITH BIOLOGICALLY ACTIVE SUBCORTEXES." Animal Biology 18, no. 2 (July 2016): 144–51. http://dx.doi.org/10.15407/animbiol18.02.144.

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Vedmed, I. V., V. I. Sheremeta, and V. G. Kaplunenko. "MELLIFEROUS CAPACITY OF BEE FAMILIES DEPENDING ON THE QUALITY OF QUEEN BEES, DERIVED WITH USING OF BIOLOGICALLY ACTIVE FEEDING." Animal Breeding and Genetics 51 (March 28, 2018): 296–302. http://dx.doi.org/10.31073/abg.51.40.

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An experiment was conducted in order to detect the influence of quality of queen bees of the Carpathian bee breed derived with using of biologically active substances on melliferous capacity of bee family, it was formed two experimental and control groups of twenty-five breed of bees. Bees breeds formed in June by the generally accepted rules were used. For the formation of group, analogical couples of bees breeds in strength, the number of sealed brood, honey and bee cerago were taken. The best queen bees’ sisters by development obtained from spring 2015 were sat to the experimental group. It means that they were genotype analogues and analogical couples by weight and body length. Queen bees derived from queen bees families which were fed with biologically active feeding Apistimulin BM and Nanostimulin were introduced to the first and second research groups. Queen bees derived from queen bees families which were fed only with sugar syrup were introduced into breeds of control group. Introducing queen bees in breeds was carried out by using conventional method of Titov’s cage. The experimental bee families were in the beehives of the similar construction in equal conditions of care and feeding. Bee families were taken to honey harvest of linden and sunflower during the season. After finishing of honey harvest from each plant, pumping honey was conducted. Determination of the amount received from each of honey bee families was performed by the method of conducting experiments in beekeeping. The influence of queen bees quality on melliferous capacity was judged by indicators of biometric processing of data from the output of trade honey during the season 2015, both separately for each breeds and overall. Honey harvest of linden from June 26 to July 5 was held under very unfavourable weather conditions for the allocation of nectar caused by high temperatures. Also unfavourable weather conditions for honey harvest were during sunflower blooming from July 19 to August 11. During flowering sunflowers, especially in the beginning, there were heavy rains, with the air temperature significantly reduced, and since the middle to the end of honey harvest it has become too high. All climatic factors influenced the quality and quantity of honey harvest. Analysis of the data showed that bees breeds formed on the basis of queen bees derived by using biologically active substances had more honey harvest than queen bees derived under usual conditions of feeding with sugar syrup. Thus, during honey harvest of linden, bees breeds of research groups have brought significantly more marketable honey by 16.4% and 51.5% than in the control. The best result was obtained in the first experimental group formed by the queen bees derived by using stimulating bioactive feeding Apistimulin BM. The results of this group were 51.5% and 23.2% higher than in the control group and second group and received an average 14.71 kg of honey of bee family. Bees breeds of the second experimental group increased honey harvest by 16.4% that is a good result for increasing the output of marketable honey in the apiary. The first experimental group had the lowest coefficient of variability, which indicates the similarity of queen bees and positive impact on their quality of honey harvest. The greatest individual features of queen bees were shown in families formed on the basis of queen bees derived by using stimulating bioactive feeding Nanostimulin. This group had the highest coefficient of variability. During honey harvest of sunflower, the number of honey exceeded the indicators of the control ones by 17.5% and 52.4%. The smallest effect was manifested in families of the second experimental group and was 17.5%. The largest melliferous capacity was showed by bee families of the first group and it was 52.4% higher, in average 16.78 kg of honey per each family. General results of marketable honey production by research bee families have confirmed significant influence of the quality of queen bees on the honey harvest. Thus, during the season 2015, the first and second research groups of bee families gathered 52% and 17% honey higher than the control ones. So, developed biotechnological method, the essence of which is feeding queen bees families with the bioactive feeding in the embryonic period, provided high-quality queen bees whose families under adverse weather conditions for honey harvest had the best melliferous capacity. It was established that bee families formed by using queen bees derived from queen bees families which were fed with bioactive feeding Nanostimulin and ApistimulinBM had 17% and 52% higher melliferous capacity under adverse weather conditions during honey harvest. Melliferous capacity of bee families depends upon the individual productive qualities of queen bees. At that, queen bees derived by using feeding ApistimulinBM are the most similar and their families have higher melliferous capacity than derived by using Nanostimulin and by generally accepted method.
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Rangel, Juliana, and Adrian Fisher. "Factors affecting the reproductive health of honey bee (Apis mellifera) drones—a review." Apidologie 50, no. 6 (September 12, 2019): 759–78. http://dx.doi.org/10.1007/s13592-019-00684-x.

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Abstract In the honey bee, Apis mellifera, colonies are composed of one queen, thousands of female workers, and a few thousand seasonal males (drones) that are reared only during the reproductive season when colony resources are plentiful. Despite their transient presence in the hive, drones have the important function of mating with virgin queens, transferring their colony’s genes to their mates for the production of fertilized, worker-destined eggs. Therefore, factors affecting drone health and reproductive competency may directly affect queen fitness and longevity, having great implications at the colony level. Several environmental and in-hive conditions can affect the quality and viability of drones in general and their sperm in particular. Here we review the extant studies that describe how environmental factors including nutrition, temperature, season, and age may influence drone reproductive health. We also review studies that describe other factors, such as pesticide exposure during and after development, that may also influence drone reproductive quality. Given that sperm development in drones is completed during pupation prior to adult emergence, particular attention needs to be paid to these factors during drone development, not just during adulthood. The present review showcases a growing body of evidence indicating that drones are very sensitive to environmental fluctuations and that these factors cause drones to underperform, potentially compromising the reproductive health of their queen mates, as well as the overall fitness of their colony.
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Chuda-Mickiewicz, Bożena, and Jerzy Samborski. "Effect of restricted pollen supply to colonies on the quality of reared queen bees." Acta Scientiarum Polonorum Zootechnica 18, no. 3 (January 15, 2020): 21–26. http://dx.doi.org/10.21005/asp.2019.18.3.04.

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Barton, Cheryl. "If patient safety is king, then quality is queen." Journal of Aesthetic Nursing 10, no. 10 (December 2, 2021): 439. http://dx.doi.org/10.12968/joan.2021.10.10.439.

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Parvu, Monica, Ioana Cristina Andronie, Violeta Elena Simion, Elena Mitranescu, and Laurentiu Tudor. "Quality of the queen bees raised by natural methods." Journal of Biotechnology 280 (August 2018): S40. http://dx.doi.org/10.1016/j.jbiotec.2018.06.127.

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Facchini, Elena, Maria Grazia De Iorio, Federica Turri, Flavia Pizzi, Daniela Laurino, Marco Porporato, Rita Rizzi, and Giulio Pagnacco. "Investigating Genetic and Phenotypic Variability of Queen Bees: Morphological and Reproductive Traits." Animals 11, no. 11 (October 26, 2021): 3054. http://dx.doi.org/10.3390/ani11113054.

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The quality of the honeybee queen has an important effect on a colony’s development, productivity, and survival. Queen failure or loss is considered a leading cause for colonies’ mortality worldwide. The queen’s quality, resulting from her genetic background, developmental conditions, mating success, and environment, can be assessed by some morphological measures. The study aims to investigate variability for traits that could assess the quality of the queen. Related animals were enrolled in this study. Variance components were estimated fitting a mixed animal model to collected data. Heritabilities of body and tagmata weights ranged from 0.46 to 0.54, whereas lower estimates were found for the tagmata width and wing length. Heritabilities estimated for the spermatheca diameter and volume, number of ovarioles, and number of sperms were 0.17, 0.88, 0.70, and 0.57, respectively. Many phenotypic correlations related to size were high and positive, while weak correlations were found between morphology and reproductive traits. Introducing a queen’s traits in a selection program could improve colonies’ survivability. Further research should focus on better defining the correlations between the individual qualities of a queen and her colony’s performance.
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Zhao, Huiyue, Shibonage K. Mashilingi, Yanjie Liu, and Jiandong An. "Factors Influencing the Reproductive Ability of Male Bees: Current Knowledge and Further Directions." Insects 12, no. 6 (June 7, 2021): 529. http://dx.doi.org/10.3390/insects12060529.

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Bumblebees and honeybees are very important pollinators and play a vital role in agricultural and natural ecosystems. The quality of their colonies is determined by the queens and the reproductive drones of mother colonies, and mated drones transmit semen, including half of the genetic materials, to queens and enhance their fertility. Therefore, factors affecting drone fecundity will also directly affect progeny at the colony level. Here, we review environmental and bee-related factors that are closely related to drone reproductive ability. The environmental factors that mainly affect the sperm count and the viability of males include temperature, nutrients and pesticides. In addition, the inherent characteristics of male bees, such as body size, weight, age, seminal fluid proteins and proteins of the spermathecal fluid, contribute to mating success, sperm quality during long-term storage in the spermathecae and the reproductive behaviors of queens. Based on the results of previous studies, we also suggest that the effects of somatotype dimorphism in bumblebee males on sperm quality and queen fecundity and the indispensable and exploitable function of gland proteins in the fecundity of males and queens should be given more attention in further studies.
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Hatch, S., D. R. Tarpy, and D. J. C. Fletcher. "Worker regulation of emergency queen rearing in honey bee colonies and the resultant variation in queen quality." Insectes Sociaux 46, no. 4 (November 1, 1999): 372–77. http://dx.doi.org/10.1007/s000400050159.

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De Souza, Daiana A., Klaus H. Hartfelder, and David R. Tarpy. "Effects of larval Age at Grafting and Juvenile Hormone on Morphometry and Reproductive Quality Parameters of in Vitro Reared Honey Bees (Hymenoptera: Apidae)." Journal of Economic Entomology 112, no. 5 (May 30, 2019): 2030–39. http://dx.doi.org/10.1093/jee/toz148.

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Abstract The honey bee queen plays a central role in the Apis mellifera L. (Hymenoptera: Apidae) colony, and her high reproductive capacity is fundamental for building up the workforce of a colony. Caste development in honey bee females involves elaborate physiological pathways unleashed at the beginning of the first larval instars, with juvenile hormone (JH) playing a crucial role. Here we took advantage of established in vitro rearing techniques to conduct a 2 × 2 experimental design and test initial rearing age (young vs old) and JH treatment (JH III vs solvent control) to enlighten the role of nutrient quality and JH in shaping honey bee female fertility, morphological features related to queenliness, and key physiological parameters (hemolymph vitellogenin/Vg, sugar levels, and Vg transcript levels). Our results show that while the age at initial larval rearing had major impacts on external morphology development, where younger larvae exhibited a higher probability to develop into queen-like adults morphotypes, the JH application during the larval stage improved physiological pathways related to ovary development and metabolism during the ontogenic development. We detected that the supplementation of queen larvae with JH promoted important benefits regarding queen fertility as the increase of ovariole number and vg levels at hemolymph, both crucial factors at eggs production. The data presented here provide guidance in efforts to improve honey bee queen quality, especially in light of frequent episodes of queen failures in the beekeeping industry.
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Simonne, Eric, Robert Boozer, and Amarat Simonne. "Yield, Ear Characteristics, and Consumer Acceptance of Selected White Sweet Corn Varieties in the Southeastern United States." HortTechnology 9, no. 2 (January 1999): 289–93. http://dx.doi.org/10.21273/horttech.9.2.289.

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White sweet corn (Zea mays L.) is widely grown in the southeastern United States. Although `Silver Queen' has been a popular variety in that region for over 20 years, many other varieties are now available. Selecting a variety for commercial or home production is a complex decision because varieties vary considerably with regard to field performance, ear characteristics, and eating quality. Because limited information is available on overall evaluation of sweet corn varieties, the objectives of this study were to 1) evaluate field performance, ear characteristics and eating quality of selected white sweet corn varieties, 2) globally compare varieties using an overall rank-sum index (ORSI), and 3) determine if `Silver Queen' is still the best variety or if it benefits from name recognition. Significant differences among varieties were found for most of the attributes evaluated. When a variety needs to be selected on the basis of a single group of attributes, our results suggest that the best varieties for field performance, ear characteristics and eating quality were `Even Sweeter' and `Treasure', `Silver Queen' and `Rising Star', and `Silverado', respectively. When ranks for all attributes were pooled together, the ORSI for all varieties fell within the 40 to 60 median range for ORSI. These results suggest that while marked differences between varieties can be found for a selected attribute, overall all selected varieties showed similar potential for commercial production. Panel response on sweet corn variety names and the rate of correct blind identification of `Silver Queen' suggested that while it is still among the best varieties, `Silver Queen' did benefit from name recognition.
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Sharma, Ajay, Kiran Rana, and Harish Kumar Sharma. "Selection of Apis mellifera L. Colonies for Quality Queen Rearing." International Journal of Current Microbiology and Applied Sciences 9, no. 1 (January 10, 2020): 2407–14. http://dx.doi.org/10.20546/ijcmas.2020.901.274.

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Gilley, David C., David R. Tarpy, and Benjamin B. Land. "Effect of queen quality on interactions between workers and dueling queens in honeybee ( Apis mellifera L.) colonies." Behavioral Ecology and Sociobiology 55, no. 2 (December 1, 2003): 190–96. http://dx.doi.org/10.1007/s00265-003-0708-y.

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Gülcan, Havva Nur, Halil Yeninar, and Salim Aktürk. "Effect of Number of Worker Bees in Queen Mating Nucleus on Queen Quality and Mating Nucleus Population Dynamics." Bee Studies- Apiculture Research Institute 12, no. 2 (December 31, 2020): 28–33. http://dx.doi.org/10.51458/bstd.2021.6.

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Sarate, Omprakash S. "Petrographic analysis and depositional environment of subsurface coal seams of Koyagudem area, Godavari Valley Coalfield, Telangana, India." Journal of Palaeosciences 64, no. (1-2) (December 31, 2015): 151–61. http://dx.doi.org/10.54991/jop.2015.110.

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The study area marks the south-eastern extremity of the Mulug Coal Belt of Godavari Valley Coalfield, Telangana. The Bore-hole No. KYG-353, containing Index above Queen and Queen seams, is located in north-western direction from Kothagudem Town of Khammam District. The petrographic analysis has been done to ascertain their rank by random vitrinite reflectance (Ro mean %) measurements and quality estimation through maceral analysis, in order to interpret the environment of deposition as well as economic significance. The Index above Queen Seam has vitrinite group of maceral in dominance therefore, contains vitric type of coal whereas; the top and a band in the middle part of the Queen Seam contain coal which is fusic (inertinite rich) in nature. The bottom part of this seam has shown vitrinite in abundance indicating its vitric nature. The random vitrinite reflectance (Ro mean %) study has revealed that the coals of Index above Queen and the Queen seams have attained high volatile bituminous C stage of rank, except for a coal band located between 161.44 m and 162.44 m depth range, which has reached high volatile bituminous B rank. It is also inferred that the vegetal resource of Index above Queen Seam has been deposited during oxic and anoxic moor conditions; however, a distinct change in the climatic conditions to oxic moor with intermittent moderate to high flood situation occurred in the depositional phase of the Queen Seam. The study also suggests that coal deposits in this region have significantly high economic potentials.
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Delmestri, Giuseppe, and Royston Greenwood. "How Cinderella Became a Queen." Administrative Science Quarterly 61, no. 4 (July 8, 2016): 507–50. http://dx.doi.org/10.1177/0001839216644253.

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Using a case study of the Italian spirit grappa, we examine status recategorization—the vertical extension and reclassification of an entire market category. Grappa was historically a low-status product, but in the 1970s one regional distiller took steps that led to a radical break from its traditional image, so that in just over a decade high-quality grappa became an exemplar of cultured Italian lifestyle and held a market position in the same class as cognac and whisky. We use this context to articulate “theorization by allusion,” which occurs through three mechanisms: category detachment—distancing a social object from its existing category; category emulation—presenting that object so that it hints at the practices of a high-status category; and category sublimation—shifting from local, field-specific references to broader, societal-level frames. This novel theorization is particularly appropriate for explaining change from low to high status because it avoids resistance to and contestation of such change (by customers, media, and other sources) as a result of status imperatives, which may be especially strong in mature fields. Unlike prior studies that have examined the status of organizations within a category, ours foregrounds shifts in the status and social meaning of a market category itself.
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Ma, Chuan, Buajiram Ahmat, and Jianke Li. "Effect of queen cell numbers on royal jelly production and quality." Current Research in Food Science 5 (2022): 1818–25. http://dx.doi.org/10.1016/j.crfs.2022.10.014.

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Kodagoda, Gethmini, Hung T. Hong, Tim J. O’Hare, Yasmina Sultanbawa, Bruce Topp, and Michael E. Netzel. "Effect of Storage on the Nutritional Quality of Queen Garnet Plum." Foods 10, no. 2 (February 7, 2021): 352. http://dx.doi.org/10.3390/foods10020352.

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Due to high perishability, plums are harvested at an early stage of maturity to extend postharvest storage life. Storage time and temperature can significantly affect the phytochemical and sugar composition of plums, altering their palatability and nutritional quality. In this study, variations in physiochemical properties (total soluble solids (TSS), titratable acidity (TA), color (chroma and hue angle)), phytochemical composition (total phenolic content (TPC), total anthocyanin content (TAC), and carotenoids), and sugars in three different tissues of the Queen Garnet plum (QGP) during storage at two common domestic storage temperatures (4 and 23 °C) were evaluated. There was an increase (p > 0.05) in TSS and a reduction (p < 0.05) in TA of the outer flesh at 23 °C. Chroma values of all the tissues reduced (p < 0.05) at 23 °C. At 4 °C, chroma values fluctuated between storage days. The TAC of the peel was the highest (p < 0.05) among the different tissues and continued to increase up to 10 days of storage at 23 °C (3-fold increase). At 4 °C, the highest (p < 0.05) TAC (peel) was observed after 14 days of storage (1.2-fold increase). TPC showed similar results. The highest (p < 0.05) TPC was recorded in the peel after 10 days of storage at 23 °C (2.3-fold increase) and after 14 days of storage at 4 °C (1.3-fold increase), respectively. Total carotenoids in the flesh samples at both storage temperatures were reduced (p < 0.05) after 14 days. Total sugars also decreased during storage. The results of the present study clearly showed that common domestic storage conditions can improve the nutritional quality of plums by increasing the content of bioactive anthocyanins and other phenolic compounds. However, the increase in phytochemicals needs to be counterbalanced with the decrease in total sugars and TA potentially affecting the sensory attributes of the plums.
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Ning, Ming, Fengxian Tang, Qin Zhang, Xinxin Zhao, Liping Yang, Wenchao Cai, and Chunhui Shan. "The quality of Gold Queen Hami melons stored under different temperatures." Scientia Horticulturae 243 (January 2019): 140–47. http://dx.doi.org/10.1016/j.scienta.2018.08.005.

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Philipson, Joakim. "The Red Queen in the Repository." International Journal of Digital Curation 15, no. 1 (July 22, 2020): 16. http://dx.doi.org/10.2218/ijdc.v15i1.646.

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One of the grand curation challenges is to secure metadata quality in the ever-changing environment of metadata standards and file formats. As the Red Queen tells Alice in Through the Looking-Glass: “Now, here, you see, it takes all the running you can do, to keep in the same place.” That is, there is some “running” needed to keep metadata records in a research data repository fit for long-term use and put in place. One of the main tools of adaptation and keeping pace with the evolution of new standards, formats – and versions of standards in this ever-changing environment are validation schemas. Validation schemas are mainly seen as methods of checking data quality and fitness for use, but are also important for long-term preservation. We might like to think that our present (meta)data standards and formats are made for eternity, but in reality we know that standards evolve, formats change (some even become obsolete with time), and so do our needs for storage, searching and future dissemination for re-use. Eventually, we come to a point where transformation of our archival records and migration to other formats will be necessary. This could also mean that even if the AIPs, the Archival Information Packages stay the same in storage, the DIPs, the Dissemination Information Packages that we want to extract from the archive are subject to change of format. Further, in order for archival information packages to be self-sustainable, as required in the OAIS model, it is important to take interdependencies between individual files in the information packages into account. This should be done already by the time of ingest and validation of the SIPs, the Submission Information Packages, and along the line at different points of necessary transformation/migration (from SIP to AIP, from AIP to DIP etc.), in order to counter obsolescence. This paper investigates possible validation errors and missing elements in metadata records from three general purpose, multidisciplinary research data repositories – Figshare, Harvard’s Dataverse and Zenodo, and explores the potential effects of these errors on future transformation to AIPs and migration to other formats within a digital archive.
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Pityurina, I. S., D. V. Vinogradov, F. A. Musaev, and D. V. Goncharuk. "Agro-ecological testing of varieties and qualitative characteristics of potato tubers in the conditions of the Ryazan region." IOP Conference Series: Earth and Environmental Science 979, no. 1 (February 1, 2022): 012031. http://dx.doi.org/10.1088/1755-1315/979/1/012031.

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Abstract The article presents the results of the analysis of the cultivation of potato varieties for yield and quality data. The aim of the study was to determine the technological and consumer characteristics of various varieties of potato grown in the Ryazan region. During the experiments were used potato varieties of domestic and foreign selection, which were approved for zoning in the Ryazan region. Potato was cultivated at the experimental agrotechnology station of Ryazan State Agrotechnological University in 2019-2020. As a result of research, it was revealed that only the varieties “Gala”, “Queen Anna” and “Kolobok” are classified as type C, which is suitable for cooking most culinary dishes, and accordingly they are universal. According to the quality indicators “taste and smell” and “darkening of the pulp”, the tubers of the “Gala”, “Queen Anna”, “Red Scarlett” and “Kolobok” varieties showed themselves worthily. According to the number of tubers on the bush, varieties “Queen Anna” and “Kolobok” also performed best. The highest average mass of tubers was given by the varieties “Rodrigo” and “Kolobok”.
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Purwanti, Siti, Bambang Sugiyanto, Nur Wening, and Bambang Moertono. "THE EFFECT OF QUALITY TANGIBLES, RELIABILITY, RESPONSIVENESS, ASSURANCE, EMPATY ON PATIENT SATISFACTION AT QUEEN LATIFA GENERAL HOSPITAL." International Journal of Business, Humanities, Education and Social Sciences (IJBHES) 1, no. 1 (June 26, 2019): 16–24. http://dx.doi.org/10.46923/ijbhes.v1i1.21.

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Background:Queen Latifa General Hospital is type D hospital that will be developed to be type C hospital. Thisdevelopment is done to further improve the service and claim value of BPJS to increase the welfareat Queen Latifa General Hospital, to fulfill the procurement of service facilities and to increase theconsumer satisfaction as well. It needs to do the research about how the level of patient satisfactiontowards the service of Queen Latifa General Hospital so that it is able to know about quality ofhospital service in order to be type C hospital in the future. Method:This research uses non-experimental quantitative design, with descriptive regression design, theresearch design used is cross sectional because the data collection of independent variables anddependent variable are done simultaneously at the same time, that is the quality of service towardsthe patient satisfaction at Queen Latifa General Hospital. Results and Discussion:From the results of F test in this study, it is obtained the F value is 12.32 with a significance number(P value) of 0.000. With 95% significance level, it means that variable of Tangibles, Reliability,Responsiveness, Assurance, Empathy have significant influence on patient satisfaction variable.The value of Adjusted R2 is 0.64 which means that the independent variable (Tangibles,Reliability, Responsiveness, Assurance, Empathy) can explain the dependent variable (Patientsatisfaction) of 64%, while the rest is explained by other factors which are not examined. Theresults of Multiple Regression Analysis Value of 0.29 on tangibles variable is positive, the valueof -0.24 in Reliability variable is negative, the value of -0.21 on responsiveness variable isnegative, the value of 0.64 on assurance variable is positive, and the value of 0.35 on the empathyvariable is positive, so that it can be concluded that the higher the quality of empathy, the higherthe patient satisfaction will be. Conclusion:The service quality at Queen Latifa General Hospital has been running well in terms of quality oftangibles, reliability, responsiveness, assurance, and empathy.
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Rosengren, Rainer, and Pekka Pamilo. "Sex Ratio Strategy as Related to Queen Number, Dispersal Behaviour and Habitat Quality in Formica Ants (Hymenoptera: Formicidae)." Entomologia Generalis 11, no. 3-4 (May 1, 1986): 139–51. http://dx.doi.org/10.1127/entom.gen/11/1986/139.

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Hunter, Philip. "Defence in depth — protecting the queen." Network Security 2003, no. 6 (June 2003): 17–18. http://dx.doi.org/10.1016/s1353-4858(03)00612-3.

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Munby, Julian. "Queen Elizabeth's Coaches: The Wardrobe on Wheels." Antiquaries Journal 83 (September 2003): 311–67. http://dx.doi.org/10.1017/s0003581500077726.

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The origins of the coach in England have never been clearly documented, but the very complete records of the royal wardrobe in the reign of Queen Elizabeth reveal her part in their introduction, and the extent of her lavish expenditure on the creation and repair of coaches from 1564 until her death four hundred years ago. Detailed descriptions of eleven new coaches, and the repairs to a number of gifts from her courtiers, record the astonishing richness and quality of these machines. This suggests that the furnishings, decoration and trappings of both horse and carriage were both an extension of the queen's costume, and also a significant part of the public persona of the monarch.
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49

Kovalskyi, Yu, B. Gutyj, V. Fedak, L. Kovalska, and A. Druzhbiak. "The influence of feed quality on the development and productivity of bee queens." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 23, no. 95 (December 11, 2021): 71–75. http://dx.doi.org/10.32718/nvlvet-a9510.

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Abstract:
The research aimed to study the effect of additional protein feeding of foster families with buckwheat stalks. The research was conducted during the summer period in 2020 and 2021. Ten foster families were selected for the experiment. All families are chosen by the method of analogs. Bees were kept in beehives. The uterus of the Carpathian breed took part in the investigation. Control families were fed 200 g of sugar syrup (1:1) twice daily for 8 and 21 hours. Feeding began three days before inoculation of queen cells and continued until the time of their sealing. The experimental families were fed the same amount of sugar syrup. Still, they received an additional 0.5 kg of protein feed in the form of bee pollen from buckwheat, the average protein content of which was 22.0 ± 0.32 %. Bee pollen is obtained by selection with the help of mounted on the auxiliary families of hinged pollen catchers with a diameter of the inlet of the pollen catcher lattice 5 mm. Pruning was carried out during the flowering of buckwheat in the period from morning to 13 o'clock in the afternoon. Adding bee pollination to the feed of foster families improves the growth and development of queens. Balancing the diet by nutrients increases the reproductive performance of the uterus, which is directly proportional to the level of their protein supply. When growing queens, the introduction of additional protein components of feed into the diet of foster families in the form of buckwheat leads to an increase in the maximum load of honeycomb by 11.4 %. The level of development of the pharyngeal glands in the researcher's families was 49 % higher. Balancing the diet by nutrients increases the reproductive performance of the queens of the Carpathian breed of bees by 16 % compared to the control.
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50

Bykov, Alexandr. "Influence of the bee breed of the queen-rearing colony on the variability of queen behavioural and morphological features." АгроЭкоИнфо 5, no. 53 (October 30, 2022): 31. http://dx.doi.org/10.51419/202125531.

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Abstract:
The adaptation of bees to the conditions of the climate in which they are located plays an important role in the preservation of the honeybee population. Numerous studies have shown that the exterior signs of bees carry not only the appearance, but are also closely related to the behavioral signs of bee colonies. Therefore, this study is necessary to determine the degree of transmission of pedigree traits of bee colonies. Keywords: HONEY BEE, APIS MELLIFERA, BEE, GENOTYPE, QUALITY FEATURES, PEACEFULNESS, LOYALTY, HONEY PRODUCTIVITY, PERSEVERANCE ON FRAME, SELECTION, MOTHERHOOD, BEE BREEDS, TEACHER FAMILY, MATERNAL FAMILY, FATHER’S FAMILY, SELECTION, ANALYSIS, LIPETSK REGION
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