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Dissertations / Theses on the topic 'Pyruvate decarboxylase'

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Published: 4 June 2021
Last updated: 25 July 2025

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1

Blalock, LeeAnn Talarico. "Expression of pyruvate decarboxylase in a Gram positive host Sarcina ventriculi pyruvate decarboxylase versus other known pyruvate decarboxylases /." [Gainesville, Fla.] : University of Florida, 2003. http://purl.fcla.edu/fcla/etd/UFE0002366.

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2

Cheung, Wing Yee. "A yeast pyruvate decarboxylase regulatory gene." Thesis, Imperial College London, 1985. http://hdl.handle.net/10044/1/37659.

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3

Brown, Audrey Elaine. "Constructing a recombinant model of the human pyruvate dehydrogenase complex." Thesis, University of Glasgow, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248119.

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4

Rose, Janet Elizabeth. "Mechanistic studies on glutamate decarboxylase and serine hydroxmethyltransferase." Thesis, University of St Andrews, 1993. http://hdl.handle.net/10023/14295.

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(2S)- and (2R)-Serine O-sulphate have been synthesised and shown to inactivate glutamate decarboxylase (GAD) from E. Coli. Novel methodology was developed to enable the stereospecific synthesis of (2S) and (2R)-deuteriated serine in order to probe the mechanism of inactivation. The rates of inactivation of glutamate decarboxylase by (2S)-, (2S)-[2-2H]-, (2R)- and (2R)-[2-2H]-serine O-sulphate have been measured for each of the isotopomers at a range of concentrations. From the data obtained the deuterium isotope effects were determined for each enantiomer. The inactivation by the (2S)-enantiom
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5

Green, J. B. A. "Control of pyruvate decarboxylase and phospho-glucose isomerase in yeast." Thesis, Imperial College London, 1988. http://hdl.handle.net/10044/1/47087.

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6

Buddrus, Lisa. "Creation and evaluation of a pyruvate decarboxylase dependent ethanol fermentation pathway in Geobacillus thermoglucosidasius." Thesis, University of Bath, 2017. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715253.

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Bioethanol, produced from organic waste as a second-generation biofuel, is an important renewable energy source. Here, recalcitrant carbohydrate sources, such as municipal and agricultural waste, and plants grown on land not suitable for food crops, are exploited. The thermophilic, Gram-positive bacterium Geobacillus thermoglucosidasius is naturally very flexible in its growth substrates and produces a variety of fermentation products, including lactate, formate, acetate and ethanol. TMO Renewables Ltd. used metabolic engineering to enhance ethanol production, creating the production strain TM
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7

Alcover, Fortuny Natàlia. "Asymmetric synthesis of chiral amines using transaminases: a multienzymatic approach by pyruvate decarboxylase coupling." Doctoral thesis, Universitat Autònoma de Barcelona, 2021. http://hdl.handle.net/10803/671815.

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La present tesi es centra en el desenvolupament i optimització d’una estratègia basada en la biocatàlisi per a la síntesi d’amines quirals, les quals són compostos òpticament actius de gran valor que poden ésser utilitzats per a la síntesi de nombrosos productes, especialment en les indústries farmacèutica i agroquímica. Més concretament, es pretén sintetitzar 3-amino-1-fenilbutà (3-APB) i 1-feniletilamina (1-PEA) a través de la reacció en cascada de la transaminasa (TA) i la piruvat decarboxilasa (PDC). Aquesta cascada es basa en una síntesi asimètrica que parteix de les seves corresponents c
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8

Bornemann, Stephen. "Studies on pyruvate decarboxylase-catalysed acyloin formation and the effects of surfactants on lipase-catalysed hydrolysis of esters." Thesis, University of Warwick, 1992. http://wrap.warwick.ac.uk/110304/.

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The effect of surfactants on the hydrolysis of achiral and chiral substrates by crude and purified porcine pancreatic lipase (PPL; EC 3.1.1.3) has been studied. Rather than accelerating the reactions, surfactants slowed down ("inhibited") the reactions relative to the rate in the absence of surfactant, despite effective emulsification of the substrate. Surfactants varied in the extent to which the reaction was inhibited and inhibition occurred below the critical micelle concentration of surfactants. Inhibition was accompanied by a loss of enantioselectivity with the crude enzyme but not the pu
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9

Leksawasdi, Noppol Biotechnology &amp Biomolecular Sciences (BABS) UNSW. "Kinetics and modelling of enzymatic process for R-phenylacetylcarbinol (PAC) production." Awarded by:University of New South Wales. Biotechnology and Biomolecular Sciences (BABS), 2004. http://handle.unsw.edu.au/1959.4/20846.

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R-phenylacetylcarbinol (PAC) is used as a precursor for production of ephedrine and pseudoephedrine, which are anti-asthmatics and nasal decongestants. PAC is produced from benzaldehyde and pyruvate mediated by pyruvate decarboxylase (PDC). A strain of Rhizopus javanicus was evaluated for its production of PDC. The morphology of R. javanicus was influenced by the degree of aeration/agitation. A relatively high specific PDC activity (328 U decarboxylase g-1 mycelium) was achieved when aeration/agitation were reduced significantly in the latter stages of cultivation. The stability of partially
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10

Acar, Seyda. "Biochemical And Genetic Studies On The Pyruvate Branch Point Enzymes Of Rhizopus Oryzae." Phd thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12604762/index.pdf.

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Rhizopus oryzae is a filamentous fungi which produces lactic acid and ethanol in fermentations. R. oryzae has numerous advantages for use industrial production of L-(+)-lactic acid but the yield of lactic acid produced on the basis of carbon consumed is low. Metabolic flux analysis of R. oryzae has shown that most of the pyruvate produced at the end of the glycolysis is channelled to ethanol, acetyl-CoA and oxaloacetate production. This study aimed to answer some questions addressed on the regulation of pyruvate branch point in R. oryzae and for this purpose biochemical characterisation of the
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11

Vançan, Susan Ienne da Silva. "Phytomonas serpens: caracterização da piruvato/indolpiruvato descarboxilase e funcionalidade da auxina produzida." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-20092012-085846/.

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Um gene que codifica uma piruvato/indolpiruvato descarboxilase (PDC/IPDC) está presente no tripanossomatídeo de plantas Phytomonas serpens. A PDC atua na fermentação alcoólica, enquanto que a IPDC atua na biossíntese do fitormônio ácido indol-3-acético (AIA). Análises filogenéticas indicam que a PDC/IPDC de P. serpens é monofilética com IPDCs de gama-proteobactérias, sugerindo um evento de transferência horizontal gênica. A análise de meios de cultura de P. serpens confirma a produção de etanol e AIA. A funcionalidade do fitormônio foi confirmada em ensaios de alongamento de hipocótilos de tom
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12

Paulikat, Mirko. "Computational Studies of ThDP-Dependent Enzymes." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E5EB-D.

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13

Stevenson, Bradley James. "Directed evolution of pyruvate decarboxylase for in vitro glycolysis." Phd thesis, 2006. http://hdl.handle.net/1885/151112.

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14

Eram, Seyed Mohammad. "Investigation of enzymes catalyzing the production of acetaldehyde from pyruvate in hyperthermophiles." Thesis, 2012. http://hdl.handle.net/10012/7029.

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Extreme thermophiles and hyperthermophiles are microorganisms capable of growing optimally at 65-79??C and 80??C plus, respectively. Many of the enzymes isolated from them are thermostable, which makes them a potential resource for research and industrial applications. An increasing number of hyper/thermophiles is shown to be able to produce ethanol as an end-metabolite. Despite characterization of many alcohol dehydrogenases (ADHs) with a potential role in the production of ethanol, to date there has been no significant progress in identifying the enzymes responsible for the production of ace
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15

Rahman, Musrur. "Manipulation of the levels of pyruvate decarboxylase and alcohol dehydrogenase for submergence tolerance in rice." Phd thesis, 2000. http://hdl.handle.net/1885/12538.

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Rice plants are partially or completely submerged when fields are flooded. During submergence, rice plants encounter anaerobic conditions, and suffer severe injury, often death, leading to major crop losses in countries affected by monsoonal flooding. Pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) catalyse thanol fermentation (EF), the major energy-producing pathway under conditions of low oxygen and are among the anaerobic polypeptides induced under such conditions. The importance of EF is emphasised by the reduced survival and germination of the ADH null mutants of maize, bar
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16

Rogers, Megan P. "Investigation of the Evolutionary Aspects of Thiamin Diphosphate-Dependent Decarboxylases." Thesis, 2015. http://hdl.handle.net/1805/7920.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>Thiamin diphosphate (ThDP)-dependent enzymes catalyze a wide range of reactions including the oxidative and nonoxidative decarboxylation of 2-keto acids, carboligation reactions, the cleavage of C-C bonds, and the formation of C-S, C-N, and C-O bonds. Surprisingly, given this diversity, all ThDP-dependent enzyme catalyzed reactions proceed through essentially the same intermediate. This suggests that these enzymes share a common ancestry and have evolved to become the diverse group of enzymes seen today. Sequence alignments have r
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17

Taylor, Stephanie Michelle 1985. "Biosynthesis of coenzyme M and the catabolism of halogenated aromatic compounds." Thesis, 2012. http://hdl.handle.net/2152/28462.

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Methanogens, members of the domain Archaea, are unique in their ability to reduce carbon substrates to methane. Coenzyme M (CoM) is required in all methanogenic pathways. The biosynthesis of this coenzyme has been well studied in Class I Methanogens, but in Class II Methanogens, such as Methanosarcina acetivorans, little is known. The first step in the biosynthetic pathway might be catalyzed by cysteate synthase (CS), which converts phosphoserine to cysteate by the addition of sulfite. The 46 kDa enzyme was successfully purified from inclusion bodies and characterized. The identity of the p
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18

Lagoas, Catarina Marreiros. "A broad evolutionary perspective of alcoholic fermentation in a non-conventional yeast clade." Master's thesis, 2021. http://hdl.handle.net/10362/132847.

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The evolution of alcoholic fermentation in the non-conventional Wickerhamiella/Starmerella (W/S) yeast clade is characterized by the loss of native pyruvate decarboxylase (PDC1) and alcohol dehydrogenase (ADH) genes. In some species, the reacquisition of this via was achieved through horizontal gene transfer (HGT) of ADH and co-option of a native decarboxylase, Aro10. This work aimed to build on the previous knowledge regarding alcoholic fermentation in the W/S clade, by combining in silico data obtained from newly sequenced genomes, with phenotypic assays, aiming to assess alcoholi
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19

Guo, Youzhong 1974. "A snapshot of the unity and diversity of biological systems at the level of chemistry : structural and mechanistic studies of Cg10062, a homologue of cis-3-chloroacrylic acid dehalogenase, FG41 malonate semialdehyde decarboxylase and the catalytic domain of pyruvate dehydrogenase phosphatase 1." Thesis, 2010. http://hdl.handle.net/2152/ETD-UT-2010-05-756.

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The tautomerase superfamily is composed of a group of proteins characterized by two key features: the N-terminal proline and a beta-alpha-beta-motif. This superfamily has been divided into five families represented by 4-oxalocrotonate tautomerase (4-OT), 5-(carboxymethyl)-2-hydroxymuconate isomerase (CHMI), cis-3-chloroacrylic acid dehalogenase (cis-CaaD), malonate semialdehyde decarboxylase (MSAD), and macrophage migration inhibitory factor (MIF). Cg10062 is a homologue of cis-CaaD, but has several distinct biochemical properties from cis-CaaD. For example, Cg10062 can be irreversibly inhibit
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