Dissertations / Theses on the topic 'PV string'

Xanthomonas oryzae pv. oryae (Xoo) est l'agent causal de la bactériose vasculaire du riz (BLB), maladie entraînant des pertes de rendement importantes dans la plupart des régions rizicoles, notamment en Afrique. La virulence des souches Asiatiques de Xoo dépend des effecteurs de la famille AvrBs3/PthA ou TAL (pour Transcription Activator-Like). Des études appro fondies sur le mode d'action des effecteurs TAL ont montré que l'activité de virulence ou d'avirulence que les TAL confèrent à la bactérie dépend essentiellement de leur interaction avec les gènes de sensibilité et/ou de résistance correspondants chez le riz. Les souches de Xoo originaires d'Afrique isolées récemment ne présentent que huit effecteurs de type TAL dans leur génome et leur rôle dans la virulence est jusqu'à présent inconnu. Les travaux de cette thèse ont porté sur la caractérisation des effecteurs de type TAL dans la souche africaine de Xoo BAI3. Une mutagenèse systématique par recombinaison homologue sur l'ensemble des huit gènes tal de la souche BAI3 a été effectuée et a conduit à l'identification et à la caractérisation du gène talC. TalC se caractérise par 21.5 répétitions et est phylogénétiquement proche des TAL de Xanthomonas oryzae pv. oryzicola (Xoc). Le mutant BAI3ΔtalC est incapable de développer les symptômes de la maladie sur plante sensible. Toutefois, les bactéries se multiplient très bien au niveau de l'apex foliaire proche du site d'inoculation, laissant supposer que TalC est requis pour la colonisation du système vasculaire. Parmi les cibles directes potentielles identifiées via l'analyse du transcriptome de feuilles de riz sensible (BAI3 versus BAI3ΔtalC), Os11N3 code une protéine de la famille des noduline-3 MtN3 et est fortement induit durant l'infection par la souche sauvage BAI3. Nous avons identifié dans la région promotrice de Os11N3 une séquence nucléotidique ciblée par TalC et démontré qu'elle était fonctionnelle via des expériences d'expression en trans dans N. benthamiana. Les travaux de cette thèse montrent pour la première fois que les effecteurs de type TAL contribuent de manière importante à la virulence de la souche africaine Xoo BAI3. Ces travaux contribueront à l'amélioration génétique des lignées de riz pour la résistance à la bactériose vasculaire du riz en Afrique
Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of Bacterial Leaf Blight (BLB) on rice, a serious disease causing important yield losses in the main rice growing regions including Africa. The virulence of Asian Xoo strains mainly depends on the type III effectors of avrBs3/pthA gene family, namely TAL (for Transcription Activator Like) effectors. In depth studies on the function of TAL effectors revealed that the virulence and/or the avirulence activities conferred by these effectors requires the binding and the induction of the corresponding S and/or R genes. African Xoo strains was shown to harbor 8 TAL effectors in their genomes. However, the contribution of these TAL effectors to Xoo virulence is still unknown. This work reports on the identification and characterization of TAL effectors in the African Xoo strain BAI3R. A random mutagenesis based on homologous recombination in the genes encoding TAL effector was conducted in Xoo str ain BAI3R and led to the identification of talC. TalC harbors 21.5 repeats in its central domain and is phylogenetically more related to TAL effectors of Xanthomonas oryzae pv. oryzicola (Xoc). The BAI3RΔtalC mutant is seriously impaired in its virulence on susceptible rice varieties. Interestingly, bacteria are still able to grow at wild-type levels in the apex of the leaf, suggesting a requirement of talc for vascular colonization. Potential direct host targets were identified by conducting a transcriptomic analysis of rice leaves challenged with Xoo strain BAI3R vs. BAI3RΔtalC. Among the identified targets, the rice gene Os11N3 was found to be highly induced upon infection by the wild type strain but not the mutant one. A DNA target box for TalC was located in the Os11N3 upstream region and proved to be functional using GUS assays. We also show that the Os11N3 341-bp upstream region is transcriptionnally activated by TalC. Our results demonstrated for the first time that TAL effectors play an important role in the virulence of Xoo strain BAI3R. Our work will contribute to better improve rice for resistance to bacterial leaf blight

Thesis (Ph. D.)--University of Florida, 1999.
Title from first page of PDF file. Document formatted into pages; contains vi, 121 p.; also contains graphics (some colored). Vita. Includes bibliographical references (p. 108-120).

An RND (resistance-nodulation-cell division) transporter, called the PseC protein, was identified at the left border of the syr-syp genomic island of Pseudomonas syringae pv. syringae strain B301D. The PseC protein exhibited amino acid homology to a putative RND transporter of Ralstonia solanacearum with identities of 61% (i.e., PseC). The pseC mutant strain showed a larger reduction in syringopeptin secretion (67%) than syringomycin secretion (41%). A β-glucuronidase assay with a pseA::uidA reporter construct indicated that the GacS/A two-component system controls expression of the pseA gene. Expression of the sypA gene by mutant strain B301D-HK4 corresponded to approximately 13% of that by parental strain B301D, whereas the syrB1 gene expression by mutant strain B301D-HK4 was nearly 61%. Mutant strain B301D-HK4 was reduced in virulence by about 58% as compared to parental strain B301D. A drug-supersensitive acrB mutant of E. coli showed increased resistance to acriflavine and tetracycline upon heterologous expression of the pseA, pseB, and pseC genes. Thus, the PseC protein, an RND transporter, has an important role in secretion of syringomycin and syringopeptin. An ATP-binding cassette (ABC) transporter, called the PseF protein, was identified at the left border of the syr-syp genomic island. The PseF protein exhibited amino acid homology to a putative ABC transporter of E. coli W3104 with identities of 57.6% (i.e., PseF to MacB). The pseF mutant strain showed significant reduction in secretion of syringomycin (74%) and syringopeptin (71%). Expression of the sypA gene by mutant strain B301D-HK7 was approximately 6.9% as compared to that of parental strain B301D, while the syrB1 gene expression by mutant strain B301D-HK7 was nearly 14.6%. Mutant strain B301D-HK7 was less virulent by approximately 67% than parental strain B301D. Expression of the pseF gene was induced approximately six times by strain B301D grown on SRMAF, as compared to that of strain B301D grown on SRM. During infection of bean plants by P. syringae pv. syringae strain B728a, expression of the pseF gene increased 3 days after inoculation. Thus, the PseF protein, an ABC transporter, responsible for secretion of syringomycin and syringopeptin is required for full virulence.

La bactériose vasculaire du riz (BLB) et à stries foliaires (BLS) causées respectivement par Xanthomonas oryzae pv. oryzae (Xoo) et X. oryzae pv. oryzicola (Xoc) sont deux maladies émergentes en Afrique de l'Ouest, suite à l'expansion de la culture du riz et à l'introduction de variétés à haut rendement au cours de ces dernières décennies. Trois nouvelles races de Xoo ont été caractérisées en Afrique dont on a montré, sur la base d'une analyse génétique, leur spécificité africaine. En revanche, une étude réalisée sur une dizaine de souches de Xoc isolées au Mali en 2003, démontre qu'elles sont apparentées à des souches de Xoc asiatiques. En Asie, plusieurs gènes de résistance à Xoo ont été identifiés et déployés dans les programmes de lutte contre BLB. Cependant aucun gène de résistance à Xoc n'a été encore identifié chez le riz. Les objectifs de notre étude étaient (i): d'implémenter les collections de souches de Xoo et Xoc Africaines disponibles mais incomplètes, à l'aide de nouvelles campagnes d'échantillonage réalisées de 2009 à 2012 dans différentes zones agroécologiques du Burkina Faso et du Mali, (ii) de déterminer la diversité génétique de ces souches, (iii) d'identifier et caractériser de nouvelles sources de résistance contre BLB et BLS au sein d'accessions de riz cultivées au Burkina Faso. Nos résultats ont montré que les souches africaines de Xoc sont hautement variables tant d'un point de vue génétique que du pouvoir pathogène. L'analyse par PCR de deux effecteurs de types III conservés (xopAJ et xopW) permet de différencier les souches de Xoc en deux groupes, xopAJ étant absent dans la majorité des souches et une insertion de 1050 bp étant détectée dans la séquence codante de xopW de certaines souches. Néanmoins, il apparait que la forte diversité génétique des Xoc n'est pas corrélée à leur origine géographique, ni à la période de collecte, ou à la nature de l'hôte. Les souches de Xoo caractérisées appartiennent toutes à la race A1 qui n'avait pas encore été signalée au Mali. Au regard de la diversité des souches et de leur évolution, il est important d'envisager un plan de surveillance épidémiologique à plus large échelle des populations de Xo dans les régions concernées en Afrique de l'Ouest. Enfin, nous avons montré que certaines variétés de riz cultivées au Burkina Faso présentent un phénotype de résistance spécifique des souches africaines de Xoo et ce, à tous les stades de développement de la plante. Ces données originales contrastent par rapport au phénotype des lignées de riz résistantes de référence (Xa4, xa5 et Xa7 efficaces uniquement au stade de tallage maximum). Eu égard à l'absence de gènes de résistance dans le riz efficaces contre Xoc, ces variétés qui constituent également une source de résistance efficaces contre la diversité des souches de Xoc africaines, offrent potentiellement un nouveau moyen pour assurer le contrôle du BLS au Burkina Faso et éventuellement dans d'autres pays Africains
Bacterial Leaf Blight (BLB) and Bacterial Leaf Streak (BLS) diseases respectively caused by Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae. pv oryzicola (Xoc) are two emerging diseases of rice in West Africa, due to the recent expansion of rice cultivation and introduction of improved rice varieties over the last decade. Three news Xoo races were characterized based on genetic analysis, demonstrating their african specificity. In contrast, a study achevied on about ten Xoc strains isolated in Mali in 2003, show that they are related to asian Xoc strains. In Asia, several R genes against Xoo have been identified and deployed in breeding program to control BLB. In contrast, no R gene against Xoc has been identified in rice. The objectives of this PhD thesis are to (i) complete the Xo collections of African isolates upon annual sampling operated from 2009 to 2012 in various agroecological areas of Burkina Faso and Mali, (ii) determine the genetic diversity of these strains , (iii) identify and characterize news sources of resistance genes to BLB and BLS within rice accessions cultivated in Burkina Faso.Our results showed that african Xoc are highly diverse genetically and phenotypically. PCR-based analyse of two conserved type III effector gene (xopAJ and xopW) differentiated two groups of Xoc strains, with xopAJ not detected in a majority of African Xoc strains and 1050 bp insertion detected in xopW gene for few strains. However, the high genetic diversity observed among the Xoc strains is not correlated to geographical origin, sampling data or host plant species. Xoo strains characterized belong all to race A1 previously reported by Gonzalez et al. (2007) in Burkina Faso. Given the diversity of X. oryzae strains and their evolution, it is essential to establish a large scale epidemiological monitoring of Xo populations in concerned regions in west Africa.At last, some accessions cultivated in Burkina Faso showed specific resistance to african Xoo strains at all plant development stages. These original data contrast with rice lines carring Xa4, xa5 and Xa7 resistance genes against BLB, which are only effective at maximun tillering stage.Given no sources of effective resistance genes against BLS is available in rice, these accessions which were also efficient against a set of Xoc strains representative of the diversity in Africa, represent a huge potential source for the control of BLS in Burkina Faso, and eventually in others african countries

Thesis (PhD)--Stellenbosch University, 2001.
ENGLISH ABSTRACT: Bacterial canker of stone fruit, caused by Pseudomonas syringae pv. syringae, is one of the most destructive crop diseases in South Africa. Chemical control has failed completely and effective long-term management strategies will have to rely on the breeding of resistant host trees. To assist in such breeding programmes, investigations into the molecular basis of the interaction between P. s. pv. syringae and stone fruit trees have been undertaken in collaboration with the ARC-Fruit, Wine and Vine Research Institute in Stellenbosch. The aim of this dissertation was to clone and identify genes that are involved in interaction between the bacterial canker pathogen and stone fruit trees. In the first part of the study, the harpin encoding gene of a local strain of the pathogen, P. s. pv. syringae NV, was amplified in a polymerase chain reaction (PCR) strategy with primers based on the hrpAZB sequences of the bean pathogen, P. s. pv. syringae 61. Sequencing of this hrpZpssNvgene revealed a high degree of homology (96%) between the harpin encoding genes and harpin proteins of the two strains. The hrpZpssNvgene was subsequently cloned into the pMAL-c2 expression vector and expressed in Escherichia co/i. This system was used for the production of purified, biologically active, recombinant HrpZpSSNV protein. In the second part of the study, differential display (DD) technology was used to identify genes that are induced in stone fruit trees in response to P. s. pv. syringae and/or its harpin elicitor. For this purpose, actively growing shoots of two Prunus sa/icina cultivars, the moderately resistant cv. 'Laetitia' and the highly susceptible cv. 'Songold' were treated with recombinant harpinpssNvprotein or live P. s. pv. syringae NV bacteria. An untreated control and wounding control was included in the experiment. Total RNA was isolated for comparative mRNA analysis 24 hours after treatment. DD profiles were generated with fifteen primer combinations. Eight candidate bands were re-amplified, cloned and sequenced. Reverse transcription PCR was employed to verify the expression patterns of the cloned bands in the original RNA sample set. Two bands, DDc and DD4 were shown to be differentially expressed between treatments and/or cultivars, while no differences in the expression levels of the remaining six bands (DDa, DDe, DD3, DD5, DD6 and DD7) were observed. BLAST similarity searches yielded significant matches for DDe, DD4 and DD7 with plant defense-related genes.
AFRIKAANSE OPSOMMING: Bakteriese kanker van steenvrugte, wat deur Pseudomonas syringae pv. syringae veroorsaak word, is een van die mees verwoestende siektes van landbougewasse in Suid-Afrika. Chemiese beheermaatreëls het geheel en al misluk en effektiewe langtermyn beheerstrategieë sal op die teling van weerstandbiedende gasheerbome moet staatmaak. Ondersoeke na die molekulêre basis van die interaksie tussen P. s. pv. syringae en steenvrugbome is in samewerking met die LNR-Vrugte-, Wyn- en Wingerdnavorsingsinstituut in Stellenbosch van stapel gestuur om tot sulke telingsprogramme by te dra. Die doelwit van hierdie proefskrif was om gene wat betrokke is in die interaksie tussen die bakteriese kanker patogeen en steenvrugbome te kloneer en te identifiseer. In die eerste gedeelte van die studie is die harpien-koderende geen van 'n plaaslike ras van die patogeen, P. s. pv. syringae NV, geamplifiseer in 'n polimerase kettingreaksie (PKR)-strategie met peilers wat op die hrpAZB-geenopeenvolgings van die boontjiepatogeen, P. s. pv. syringae 61, gebaseer is. Volgordebepaling van hierdie hrpZpssNv-geen het 'n hoë vlak van homologie (96%) tussen die harpien-koderende gene en harpien proteïene van die twee rasse getoon. Die hrpZpssNv-geen is vervolgens in die uitdrukkingsvektor pMAL-c2 gekloneer en uitgedruk in Escherichia coli. Hierdie sisteem is vir die produksie van suiwer, biologies-aktiewe, rekombinante HrpZpssNv-proteingebruik. In die tweede gedeelte van die studie is die differensiaalvertoon (DD) tegniek gebruik om gene te identifiseer wat deur P. s. pv. syringae en/of sy harpien elisitar in steenvrugbome geïnduseer word. Vir hierdie doel is aktief-groeiende lote van twee Prunus sa/icina kultivars, die matig weerstandbiedende kv. 'Laetitia' en die hoogs vatbare kv. 'Songold', met rekombinante harpienpssNvproteïen of lewende P. s. pv. syringae NV bakterieë behandel. 'n Onbehandelde- en verwondingskontrole is in die eksperiment ingesluit. Totale RNA is 24 uur na behandeling vir vergelykende mRNA-analise geïsoleer. DD-profiele is met vyftien peilerkombinasies gegenereer. Agt kandidaatbande is geheramplifiseer en gekloneer, waarna hul DNA-opeenvolgings bepaal is. Trutranskriptase-PKR is gebruik om die ekspressiepatrone van die gekloneerde bande in die oorspronklike RNA monsters na te gaan. Daar is vasgestel dat twee van die bande, DDc en DD4, differensieel tussen kultivars en/of behandelings uitgedruk is, terwyl geen verskille in die ekspressievlakke van die oorblywende ses bande (DDa, DOe, 003, DOS, 006 en DO7) waargeneem is nie. BLAST-soektogte het betekenisvolle ooreenkomste vir DDe, DD4 en DD7 met plant weerstandsgeassosieerde gene opgelewer.

abstract: Two major challenges in the transformer-less, single-phase PV string inverters are common mode leakage currents and double-line-frequency power decoupling. In the proposed doubly-grounded inverter topology with innovative active-power-decoupling approach, both of these issues are simultaneously addressed. The topology allows the PV negative terminal to be directly connected to the neutral, thereby eliminating the common-mode ground-currents. The decoupling capacitance requirement is minimized by a dynamically-variable dc-link with large voltage swing, allowing an all-film-capacitor implementation. Furthermore, the use of wide-bandgap devices enables the converter operation at higher switching frequency, resulting in smaller magnetic components. The operating principles, design and optimization, and control methods are explained in detail, and compared with other transformer-less, active-decoupling topologies. A 3 kVA, 100 kHz single-phase hardware prototype at 400 V dc nominal input and 240 V ac output has been developed using SiC MOSFETs with only 45 μF/1100 V dc-link capacitance. The proposed doubly-grounded topology is then extended for split-phase PV inverter application which results in significant reduction in both the peak and RMS values of the boost stage inductor current and allows for easy design of zero voltage transition. A topological enhancement involving T-type dc-ac stage is also developed which takes advantage of the three-level switching states with reduced voltage stress on the main switches, lower switching loss and almost halved inductor current ripple. In addition, this thesis also proposed two new schemes to improve the efficiency of conventional H-bridge inverter topology. The first scheme is to add an auxiliary zero-voltage-transition (ZVT) circuit to realize zero-voltage-switching (ZVS) for all the main switches and inherent zero-current-switching (ZCS) for the auxiliary switches. The advantages include the provision to implement zero state modulation schemes to decrease the inductor current THD, naturally adaptive auxiliary inductor current and elimination of need for large balancing capacitors. The second proposed scheme improves the system efficiency while still meeting a given THD requirement by implementing variable instantaneous switching frequency within a line frequency cycle. This scheme aims at minimizing the combined switching loss and inductor core loss by including different characteristics of the losses relative to the instantaneous switching frequency in the optimization process.
Dissertation/Thesis
Doctoral Dissertation Electrical Engineering 2017

碩士
國立中正大學
電機工程研究所
100
This thesis presents development of two maximum power point trackers (MPPTs) for two-string PV panels in a 5 kW DC distribution system. To deal with wide output voltage range of the PV panels, the proposed MPPT topology consists of buck and boost converters. For increasing the efficiency of PV panels, a perturbation and observation algorithm is employed to track the maximum output power of the panels. Two MPPT modules and a bi-directional inverter connected with the AC grid are utilized to establish the DC distribution system. Moreover, the two maximum power point trackers can be operated in independent mode or in parallel mode. In the parallel mode, a current balancing control is adopted for the two MPPT modules. In addition, the two MPPT modules are designed with a hot-swap feature and power derating to increase the application feasibility of the two MPPT modules in the DC distribution system. Finally, experimental results have verified that the maximum power tracking accuracy can reach 99%.

abstract: In the current photovoltaic (PV) industry, the O&M (operations and maintenance) personnel in the field primarily utilize three approaches to identify the underperforming or defective modules in a string: i) EL (electroluminescence) imaging of all the modules in the string; ii) IR (infrared) thermal imaging of all the modules in the string; and, iii) current-voltage (I-V) curve tracing of all the modules in the string. In the first and second approaches, the EL images are used to detect the modules with broken cells, and the IR images are used to detect the modules with hotspot cells, respectively. These two methods may identify the modules with defective cells only semi-qualitatively, but not accurately and quantitatively. The third method, I-V curve tracing, is a quantitative method to identify the underperforming modules in a string, but it is an extremely time consuming, labor-intensive, and highly ambient conditions dependent method. Since the I-V curves of individual modules in a string are obtained by disconnecting them individually at different irradiance levels, module operating temperatures, angle of incidences (AOI) and air-masses/spectra, all these measured curves are required to be translated to a single reporting condition (SRC) of a single irradiance, single temperature, single AOI and single spectrum. These translations are not only time consuming but are also prone to inaccuracy due to inherent issues in the translation models. Therefore, the current challenges in using the traditional I-V tracers are related to: i) obtaining I-V curves simultaneously of all the modules and substrings in a string at a single irradiance, operating temperature, irradiance spectrum and angle of incidence due to changing weather parameters and sun positions during the measurements, ii) safety of field personnel when disconnecting and reconnecting of cables in high voltage systems (especially field aged connectors), and iii) enormous time and hardship for the test personnel in harsh outdoor climatic conditions. In this thesis work, a non-contact I-V (NCIV) curve tracing tool has been integrated and implemented to address the above mentioned three challenges of the traditional I-V tracers. This work compares I-V curves obtained using a traditional I-V curve tracer with the I-V curves obtained using a NCIV curve tracer for the string, substring and individual modules of crystalline silicon (c-Si) and cadmium telluride (CdTe) technologies. The NCIV curve tracer equipment used in this study was integrated using three commercially available components: non-contact voltmeters (NCV) with voltage probes to measure the voltages of substrings/modules in a string, a hall sensor to measure the string current and a DAS (data acquisition system) for simultaneous collection of the voltage data obtained from the NCVs and the current data obtained from the hall sensor. This study demonstrates the concept and accuracy of the NCIV curve tracer by comparing the I-V curves obtained using a traditional capacitor-based tracer and the NCIV curve tracer in a three-module string of c-Si modules and of CdTe modules under natural sunlight with uniform light conditions on all the modules in the string and with partially shading one or more of the modules in the string to simulate and quantitatively detect the underperforming module(s) in a string.
Dissertation/Thesis
Masters Thesis Engineering 2020

abstract: The goal of any solar photovoltaic (PV) system is to generate maximum energy throughout its lifetime. The parameters that can affect PV module power output include: solar irradiance, temperature, soil accumulation, shading, encapsulant browning, encapsulant delamination, series resistance increase due to solder bond degradation and corrosion and shunt resistance decrease due to potential induced degradation, etc. Several PV modules together in series makes up a string, and in a power plant there are a number of these strings in parallel which can be referred to as an array. Ideally, PV modules in a string should be identically matched to attain maximum power output from the entire string. Any underperforming module or mismatch among modules within a string can reduce the power output. The goal of this project is to quickly identify and quantitatively determine the underperforming module(s) in an operating string without the use of an I-V curve tracer, irradiance sensor or temperature sensor. This goal was achieved by utilizing Radiovoltmeters (RVM). In this project, it is demonstrated that the voltages at maximum power point (Vmax) of all the individual modules in a string can be simultaneously and quantitatively obtained using RVMs at a single irradiance, single module operating temperature, single spectrum and single angle of incidence. By combining these individual module voltages (Vmax) with the string current (Imax) using a Hall sensor, the power output of individual modules can be obtained, quickly and quantitatively.
Dissertation/Thesis
Masters Thesis Engineering 2019

碩士
國立中興大學
植物病理學系
86
The recent taxonomic studies showed that strains of Xanthomonascampestris pv. vesicatoria (Xcv) were heterogeneous population and weredivided into two genetically distinct groups (group A and group B). In orderto understand the variation and grouping of Xcv from Taiwan, a total of 152strains of Xcv from various localities of Taiwan were tested for theirphysiological, cell composition and molecular characteristics. Among the 152Xcv strains, 113 strains were nonpectolytic and nonamylolytic (group A) and 39strains were pectolytic and amylolytic (group B). The analysis by the systemof Biolog GN Microplate showed that strains varied greatly in utilization of anumber of carbon compounds. Cis-aconitate was the only substrate that helpedto discriminate between strains of the group A and group B. The fatty acidprofiles of 12 strains tested were qualitatively similar but quantitativedifferent. The amount of the 15:0 ante-iso fatty acid for group A strains(except XVT36) was lower than group B strains. Analyzing the whole-cellprotein patterns, strains of the same group were similar and which of thedifferent group were dissimilar. Particularly, the group A strains shared a32-33 kDa and a 24- 25 kDa protein band, whereas the group B strains sharedanother proteins with molecular weight about 26-27 kDa and 23-24 kDa. Based onthe cluster analysis of the protein profiles, though the strains of the samegroups were polymorphic, the similarity of the strains of the same group washigher than the strains of the different group. DNAs of Xcv strains weredigested with restriction enzymes Xba I and Spe I and analyzed by PFGE, theresult showed that the similarity of the DNA restriction fragments of the samegroup strains was higher than the strains of the different group. With theprimer pairs designed by previous investigators based on the DNA fragmentinvolved in the expression of two antigens associated with thelipopolysaccharide for reacting in PCR, all group A strains amplified a 560 bpproduct and group B strains did not. The homology of DNA of 5 group A strainsand 5 group B strains were analyzed by dot blot, the result showed that thesimilarity was 72~148 ﹪ for group A strains, 70~186 ﹪ for group B strainsand 12~58 ﹪ between group A and group B. The results of this study revealedthat Xcv strains from Taiwan were heterogeneous population, and can bedistinguished into two groups ( group A and group B ) which were the same asthat reported in other country. The group A belongs to Xanthomonas axonopodispv. vesicatoria and the group B was placed in Xanthomonas vesicatoria.

碩士
國立中興大學
植物病理學系所
107
Bacterial leaf streak (BLS) disease of rice, elicited by Xanthomonas oryzae pv. oryzicola (Xoc), was originally discovered in the Philippines in 1918 and recently found in Taiwan (Nantou and Yulin counties) in 2007. To understand the diversity of the BLS pathogens in Taiwan, 48 Xoc strains isolated at different time and locations were differentiated by their pathological reactions on 18 rice varieties, i.e. IR24, 12 IR24-derived IRBB lines, and 5 Taiwan commercial lines, and by PCR-amplified DNA fingerprints that primed to repetitive sequences. The genotypes of Xoc strains were determined by BOX-PCR fingerprinting that grouped the 48 Xoc strains into 4 haplotypes. Rice inoculation tests in the fields and greenhouse showed the Xoc strains caused differential virulence on the 18 rice lines, and the rice cultivars IRBB5 and IRBB7 were more resistant to the majority of the 48 Xoc strains. Nevertheless, the bacterial haplotypes were not correlated with the geographical distribution or the pathological reactions on the rice cultivars. Additional rice inoculation tests on 2-month-old rice seedlings in the greenhouse confirmed that the rice resistance phenotypes were not affected by the inoculation methods (rubbing and infiltration) or the plant age. However, some rice cultivars, e.g. TK9, exhibited differential pathological reactions between greenhouse and field assays, suggesting the rice resistance against Xoc infection may be modulated by environmental factors. The results of this research revealed the genotypic and pathological diversity of Xoc strains in Taiwan, which can be applied to develop durable resistant cultivars to rice bacterial leaf streak disease.

碩士
亞洲大學
生物科技學系碩士班
95
Xanthomonas campestris pv. campestris (Xcc) is the pathogen causing black rot in cruciferous plants. It has previously demonstrated in our laboratory that a fleQ mutant is non-flagellated and immobile and expression of flagella genes fliE, fliQ, fliL, flgG, flgB, and flhF was depended on transcriptional activator, FleQ. In this work, it is shown that expression of fliA and fliC is also regulated by FleQ. In order to know if a plasmid-encoded fleQ gene can complement a mutation in fleQ, the fleQ gene was cloned into pBBad22K carrying an arabinose inducible promoter. The derived plasmid, pBadfleQ, was introduced into mutants and the parental strain. The phenotypic and Western blot analysis demonstrated that plasmid-encoded fleQ was expressed in the absence of inducer and the small amount of FleQ produced was sufficient to complement the mobility defect of a fleQ mutant. Addition of arabinose was increased the production of FleQ but decreased the effect of complementation. Induction to overexpress FleQ by 0.05% arabinose in Xc17fleQ::Gm (pBadfleQ ) and Xc17(pBadfleQ ) hindered their growth and severely reduced the biosynthesis of extracellular polysaccharide (EPS) and extracellular enzymes, such as amylases, proteinases, and pectinases. The promoter activity of the major EPS biosynthesis gene gumB is inhibited by the overexpressed FleQ protein. Since EPS and extracellular enzymes are important virulence factors in Xcc, Xc17fleQ::Gm(pBadfleQ) and Xc17(pBadfleQ), are less virulent than their parental wild-Type strain. It has previously been show in our laboratory that RpoN, FleQ, FleN, FlhF, FlgM and FliA can regulate flagellar genes expression. In this work, yeast two-hybrid system was used to further analyze the protein-protein interaction between these regulators. Our data revealed that proteins FleN and FliA and FlgM and FliA, RpoN2 and FlhF have interaction.

All members of the genus Xanthomonas are considered to be plant pathogenic, with specific pathovars infecting several high value agricultural crops. One of these pathovars, X. campestris pv. zeae (as this is only a proposed name it will further on be referred to as Xanthomonas BLSD) the causal agent of bacterial leaf steak of maize, has established itself as a widespread significant maize pathogen within South Africa. Insufficient information about the present distribution of the pathogen is available. The main aim of the study was thus to isolate and characterise the pathogen using molecular methods. Results demonstrated that the causal agent of bacterial leaf streak disease (Xanthomonas BLSD: potentially X. campestris pv. zeae) was widely distributed within the major maize cultivation regions of South Africa. Most of the isolates collected originated from the Highveld maize production provinces (North West, Free State, Gauteng and Mpumalanga provinces) as well as from irrigated maize fields in the Northern Cape province. The XgumD gene marker was used to determine if the isolates belonged to the genus Xanthomonas. The gumD gene fragment is located within the gumB-gumM region of the operon and is conserved among Xanthomonas species. This gene fragment is partially responsible for xanthan production. This marker was amplified from all isolates and a selected number were sequenced. The marker was only able to confirm that the causal agent was a member of the genus Xanthomonas. PCR methods were used for the characterisation of the isolates. This included PCR and sequencing of ribosomal RNA- gyraseB and gumD genes. A fingerprinting method BOX-PCR was also employed. Good quality DNA of sufficient quantities was obtained from the various isolates. Amplification produced no non-specific amplification products. This resulted in good quality sequences that could be analysed using bioinformatics tools. Phylogenetic analyses of the ribosomal RNA and gyraseB genes could not detect differences amongst the 47 Xanthomonas BLSD isolates. However, these genes were able to distinguish between the type strain of these isolates and various Xanthomonas species and pathovars. From all three neighbour joining trees the Xanthomonas BLSD isolates had close association with X. axonopodis pv. vasculorum strain ATCC 35938. For the 16S rRNA gene there exists no sequence differences between Xanthomonas BLSD and X. axonopodis pv. vasculorum strain ATCC 35938. A single nucleotide difference was observed between Xanthomonas BLSD and X. axonopodis pv. vasculorum strain ATCC 35938 for the 23S rRNA gene. The gyraseB gene detected a total of six nucleotide variations between these two Xanthomonas species. For all of the phylogenetic trees there was no clustering of Xanthomonas BLSD with X. campestris pathovars. Genetic profiling (via BOX-PCR) based on present/absent analysis revealed no variations amongst the Xanthomonas BLSD isolates. All isolates shared an identical pattern produced by 12 distinct PCR products. This profiling technique did differentiate between the isolates of Xanthomonas BLSD and X. axonopodis pv. vasculorum strain ATCC 35938. Their profiles shared common bands, but differed in the number and overall pattern of the bands. These results suggest two main conclusions: (i) Xanthomonas BLSD has a clonal origin with geographical separation not impacting genetic variation. The fact that all the isolates appear to be clonal may imply that when resistant maize cultivars are developed these should be resistant to all isolates of the pathovar irrespective of their geographical origin. This is a suggestion that will have to be corroborated using more isolates and additional genetic fingerprinting techniques (ii) the Xanthomonas BLSD isolates from this study may not belong to X. campestris. Further studies using other markers should be conducted to determine the real identity of Xanthomonas BLSD.
MSc Environmental Sciences, North-West University, Potchefstroom Campus, 2015