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Published: 4 June 2021
Last updated: 6 September 2023

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1

Godfrey, S. A. C., S. A. Harrow, J. W. Marshall, and J. D. Klena. "Characterization by 16S rRNA Sequence Analysis of Pseudomonads Causing Blotch Disease of Cultivated Agaricus bisporus." Applied and Environmental Microbiology 67, no. 9 (September 1, 2001): 4316–23. http://dx.doi.org/10.1128/aem.67.9.4316-4323.2001.

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ABSTRACT Bacterial blotch of Agaricus bisporus has typically been identified as being caused by either Pseudomonas tolaasii (brown blotch) or Pseudomonas gingeri(ginger blotch). To address the relatedness of pseudomonads able to induce blotch, a pilot study was initiated in which pseudomonads were selectively isolated from mushroom farms throughout New Zealand. Thirty-three pseudomonad isolates were identified as being capable of causing different degrees of discoloration (separable into nine categories) of A. bisporus tissue in a bioassay. These isolates were also identified as unique using repetitive extragenic palindromic PCR and biochemical analysis. Relationships between these 33 blotch-causing organisms (BCO) and a further 22 selected pseudomonad species were inferred by phylogenetic analyses of near-full-length 16S rRNA gene nucleotide sequences. The 33 BCO isolates were observed to be distributed throughout thePseudomonas fluorescens intrageneric cluster. These results show that in addition to known BCO (P. tolaasii, P. gingeri, and Pseudomonas reactans), a number of diverse pseudomonad species also have the ability to cause blotch diseases with various discolorations. Furthermore, observation of ginger blotch discoloration of A. bisporus being independently caused by many different pseudomonad species impacts on the homogeneity and classification of the previously described P. gingeri.
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2

Rahman, Mizanur, Mohammad Jobayer, Nadira Akter, Farook Ahamed, SM Shamsuzzaman, and Kazi Zulfiquer Mamun. "Rapid detection of Pseudomonad at species level by multiplex PCR in surgical units and ICU of Dhaka Medical College Hospital." Bangladesh Journal of Medical Microbiology 10, no. 2 (July 28, 2016): 22–26. http://dx.doi.org/10.3329/bjmm.v10i2.51928.

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Pseudomonads are the most important gram negative organisms involved in various types of infection. This cross sectional study was conducted from January to December 2010 to isolate and identify Pseudomonad at species level in different clinical samples by culture and multiplex polymerase chain reaction (PCR) and to evaluate the efficacy of PCR in rapid detection of the bacteria at species level. Wound swab and tips of endotracheal tube were collected from hospitalized patients from different surgical units and intensive care unit (ICU) of Dhaka Medical College Hospital, Dhaka. Pseudomonads were isolated and identified at species level by culture, microscopy, different biochemical tests and PCR. Among 230 samples, 52.6% were surgical wound, 34.3% were burn wound and 9.6% were traumatic wound samples and 3.4% were tips of endotracheal tubes. Twenty six percent isolated organisms were Pseudomonas spp., 30.4% were Escherichia coli, and 13.5% were Staphylococcus aureus. Others were Proteus, Klebsiella pneumoniae, Acinetobacter baumannii and Enterobacter spp. In 19.67% samples mixed infections by other organism (Esch coli, Staph aureus, Proteus spp, Klebsiella spp) with Pseudomonas were detected and its distribution was highest in traumatic and burn wound. Multiplex PCR and different biochemical tests were used to identify 3 bacterial species of Pseudomonad. Among the species identified, 95.52% was Pseudomonas aeruginosa, 2.99% was Stenotrophomonas maltophilia and 1.49% was Burkholderia cepacia. The sensitivity of multiplex PCR was 95.08% and specificity 94.67%. PCR was the most rapid and more accurate method for detection of Pseudomonad at species level. Bangladesh J Med Microbiol 2016; 10 (2): 22-26
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3

FARRAG, SEHAM A., and ELMER H. MARTH. "Behavior of Listeria monocytogenes when Incubated Together with Pseudomonas Species in Tryptose Broth at 7 and 13°C." Journal of Food Protection 52, no. 8 (August 1, 1989): 536–39. http://dx.doi.org/10.4315/0362-028x-52.8.536.

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Tryptose broth (TB) was inoculated with Listeria monocytogenes (strain Scott A or California), Pseudomonas aeruginosa, Pseudomonas flourescens, or a combination of L. monocytogenes plus Pseudomonas species, and incubated at 7 or 13°C for 8 weeks. McBride Listeria Agar was used to determine numbers of L. monocytogenes and Pseudomonas Isolation Agar to enumerate Pseudomonas species at 0, 7, 14, 28, 42, or 56 d. At 13°C, presence of P. fluorescens had a slight negative effect on growth of L. monocytogenes strain Scott A, and was somewhat detrimental to its survival during the extended incubation. Growth of L. monocytogenes strain California was retarded by presence of P. fluorescens although the maximum population achieved by the pathogen was greater in the presence rather than absence of the pseudomonad; the pseudomonad did have a negative effect on survival of the pathogen. At the same temperature, P. aeruginosa had a negative effect on survival of L. monocytogenes strain California, but had essentially no effect on the other strain of the pathogen. Neither strain of L. monocytogenes affected growth of P. fluorescens nor P. aeruginosa. At 13°C the pH of TB generally decreased when L. monocytogenes grew by itself but increased when either pseudomonad grew by itself or together with the pathogen. At 7°C, growth of both pseudomonads was minimal. Presence of non-growing cells of P. fluorescens retarded somewhat growth of both L. monocytogenes strains early during the incubation. P. aeruginosa had no detectable effect on either strain of L. monocytogenes. The pH of TB decreased when L. monocytogenes grew by itself or together with either pseudomonad, and remained unchanged in TB inoculated with either pseudomonad.
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4

Meyer, Jean-Marie, Valérie A. Geoffroy, Nader Baida, Louis Gardan, Daniel Izard, Philippe Lemanceau, Wafa Achouak, and Norberto J. Palleroni. "Siderophore Typing, a Powerful Tool for the Identification of Fluorescent and Nonfluorescent Pseudomonads." Applied and Environmental Microbiology 68, no. 6 (June 2002): 2745–53. http://dx.doi.org/10.1128/aem.68.6.2745-2753.2002.

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ABSTRACT A total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. As a general rule, strains within a well-circumscribed taxonomic group, namely the species Pseudomonas brassicacearum, Pseudomonas fuscovaginae, Pseudomonas jessenii, Pseudomonas mandelii, Pseudomonas monteilii, “Pseudomonas mosselii,” “Pseudomonas palleronii,” Pseudomonas rhodesiae, “Pseudomonas salomonii,” Pseudomonas syringae, Pseudomonas thivervalensis, Pseudomonas tolaasii, and Pseudomonas veronii and the genomospecies FP1, FP2, and FP3 produced an identical pyoverdine which, in addition, was characteristic of the group, since it was structurally different from the pyoverdines produced by the other groups. In contrast, 28 strains belonging to the notoriously heterogeneous Pseudomonas fluorescens species were characterized by great heterogeneity at the pyoverdine level. The study of 23 partially characterized phenotypic clusters demonstrated that siderotyping is very useful in suggesting correlations between clusters and well-defined species and in detecting misclassified individual strains, as verified by DNA-DNA hybridization. The usefulness of siderotyping as a determinative tool was extended to the nonfluorescent species Pseudomonas corrugata, Pseudomonas frederiksbergensis, Pseudomonas graminis, and Pseudomonas plecoglossicida, which were seen to have an identical species-specific siderophore system and thus were easily differentiated from one another. Thus, the fast, accurate, and easy-to-perform siderotyping method compares favorably with the usual phenotypic and genomic methods presently necessary for accurate identification of pseudomonads at the species level.
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5

Mulet, Magdalena, María José Martínez, Margarita Gomila, Johanna Dabernig-Heinz, Gabriel E. Wagner, Clemens Kittinger, Gernot Zarfel, Jorge Lalucat, and Elena García-Valdés. "Genome-Based Species Diversity Assessment in the Pseudomonas chlororaphis Phylogenetic Subgroup and Proposal of Pseudomonas danubii sp. nov. Isolated from Freshwaters, Soil, and Rhizosphere." Diversity 15, no. 5 (May 2, 2023): 617. http://dx.doi.org/10.3390/d15050617.

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The Pseudomonas chlororaphis phylogenetic subgroup of species, within the Pseudomonas fluorescens group, currently includes seven bacterial species, all of which have environmental relevance. Phylogenomic analyses help clarify the taxonomy of strains in the group and allow for precise identification. Thirteen antibiotic-resistant strains isolated in a previous study from nine different sampling sites in the Danube River were suspected to represent a novel species and are investigated taxonomically in the present study, together with four other strains isolated from the Woluwe River (Belgium) that were phylogenetically closely related in their rpoD gene sequences. The strains were characterized phenotypically, chemotaxonomically (fatty acid composition and main protein profiles), and phylogenetically. They could not be assigned to any known Pseudomonas species. Three genomes of representative strains were sequenced and analyzed in the context of the genome sequences of closely related strains available in public databases. The phylogenomic analysis demonstrates the need to differentiate new genomic species within the P. chlororaphis subgroup and that Pseudomonas piscis and Pseudomonas aestus are synonyms. This taxonomic study demonstrates that 14 of the characterized isolates are members of the Pseudomonas_E protegens_A species in the GTDB taxonomy and that they represent a novel species in the genus Pseudomonas, for which we propose the name Pseudomonas danubii sp. nov. with strain JDS02PS016T (=CECT 30214T = CCUG 74756T) as the type strain. The other three strains (JDS08PS003, rDWA16, and rDWA64) are members of the species Pseudomonas_E protegens_B in the GTDB taxonomy and need further investigation for proposal as a new bacterial species.
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6

Reddy, Gundlapalli S. N., Genki I. Matsumoto, Peter Schumann, Erko Stackebrandt, and Sisinthy Shivaji. "Psychrophilic pseudomonads from Antarctica: Pseudomonas antarctica sp. nov., Pseudomonas meridiana sp. nov. and Pseudomonas proteolytica sp. nov." International Journal of Systematic and Evolutionary Microbiology 54, no. 3 (May 1, 2004): 713–19. http://dx.doi.org/10.1099/ijs.0.02827-0.

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Thirty-one bacteria that belonged to the genus Pseudomonas were isolated from cyanobacterial mat samples that were collected from various water bodies in Antarctica. All 31 isolates were psychrophilic; they could be divided into three groups, based on their protein profiles. Representative strains of each of the three groups, namely CMS 35T, CMS 38T and CMS 64T, were studied in detail. Based on 16S rRNA gene sequence analysis, it was established that the strains were related closely to the Pseudomonas fluorescens group. Phenotypic and chemotaxonomic characteristics further confirmed their affiliation to this group. The three strains could also be differentiated from each other and the closely related species Pseudomonas orientalis, Pseudomonas brenneri and Pseudomonas migulae, based on phenotypic and chemotaxonomic characteristics and the level of DNA–DNA hybridization. Therefore, it is proposed that strains CMS 35T (=MTCC 4992T=DSM 15318T), CMS 38T (=MTCC 4993T=DSM 15319T) and CMS 64T (=MTCC 4994T=DSM 15321T) should be assigned to novel species of the genus Pseudomonas as Pseudomonas antarctica sp. nov., Pseudomonas meridiana sp. nov. and Pseudomonas proteolytica sp. nov., respectively.
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7

Clark, Linda L., Joseph J. Dajcs, Celeste H. McLean, John G. Bartell, and David W. Stroman. "Pseudomonas otitidis sp. nov., isolated from patients with otic infections." International Journal of Systematic and Evolutionary Microbiology 56, no. 4 (April 1, 2006): 709–14. http://dx.doi.org/10.1099/ijs.0.63753-0.

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A novel Pseudomonas species, for which the name Pseudomonas otitidis sp. nov. is proposed, was identified from clinical specimens of infected human ears. Forty-one pseudomonads (34 from patients with acute otitis externa, six from patients with acute otitis media with otorrhoea and one from a patient with chronic suppurative otitis media) were recovered that did not match any known species. On the basis of genetic analyses and biochemical characterization, these isolates were shown to belong to the genus Pseudomonas. 16S rRNA gene sequence analysis and DNA–DNA hybridization studies indicated that this novel bacterium is closely related to, but different from, Pseudomonas aeruginosa. A description of this species is based on polyphasic studies of 11 clinical isolates. The type strain of Pseudomonas otitidis is MCC10330T (=ATCC BAA-1130T=DSM 17224T).
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8

Tryfinopoulou, P., E. Tsakalidou, and G. J. E. Nychas. "Characterization of Pseudomonas spp. Associated with Spoilage of Gilt-Head Sea Bream Stored under Various Conditions." Applied and Environmental Microbiology 68, no. 1 (January 2002): 65–72. http://dx.doi.org/10.1128/aem.68.1.65-72.2002.

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ABSTRACT The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolated from S. aurata stored under different temperatures (at 0, 10, and 20°C) and packaging conditions (air and a modified atmosphere of 40% CO2-30% N2-30% O2). Pseudomonas lundensis was the predominant species, followed by Pseudomonas fluorescens, while Pseudomonas fragi and Pseudomonas putida were detected less frequently. Fluorescent Pseudomonas strains dominated under air conditions, while proteolytic and less lipolytic strains dominated under modified-atmosphere packaging. Different storage conditions appear to govern the selection of pseudomonads in gilt-head sea bream fish.
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9

Ikram, Sadia, Saima Inam, Saba Shamim, Syed Zeeshan Haider, Muhammad Atif Qureshi, and Asma Inam. "Emerging Patterns in Antimicrobial Susceptibility Profiles of Pseudomonas SPP- Hospital Based Study." Pakistan Journal of Medical and Health Sciences 17, no. 2 (February 28, 2023): 129–31. http://dx.doi.org/10.53350/pjmhs2023172129.

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Background: There is an emerging trend of pseudomonas infections in immune-compromised patients, specifically in hospital settings. The flagship member of this family is Pseudomonas aeruginosa, which is a major infectious agent. This study assessed the distribution and susceptibility patterns of Pseudomonas species isolated from various specimens as a part of surveillance program, in order to devise antibiograms. Aim: To determine frequency & antimicrobial sensitivities of Pseudomonas species in a tertiary care hospital from Lahore. Study design: Retrospective, descriptive, cross sectional study Place & duration of study: Conducted in a tertiary care hospital in Lahore, from January 2021 to January 2022. Methods: Thirty two isolates of Pseudomonas species from different clinical specimens were isolated in microbiology section of a tertiary care hospital during a period of 13 months. MacConkey and blood agar were utilized for culturing of organisms. Gram staining, oxidase, and catalase testwere utilized for phenotypic characterization. Antibiotic susceptibility testing against anti-pseudomonal drugs was performed according to the Clinical and Laboratory Standards Institute guidelines (CLSI) 2021. Results: The inference drawn at the end of study was that, out of thirty two isolates of Pseudomonas species received and pus was the most common specimen (46.8%), followed by 18.75% from urine specimens. 62.5% of the pseudomonas species were obtained from male patients. The most affected age group was 40-60 years, followed by 1-20 years. The most sensitive options turned out to be Imipenem (65.6%) and Pipericillintazobactam (62.5%), followed by 59.3% sensitivity in Amikacin. The least sensitive options in the study isolates were Aztreonam (15.6%) and Ticarcillin clavulanic acid (25%). In 18% of pseudomonas species isolated from urine cultures, fosfomycin was 83.3%, whereas nitrofurantoin turned out to be 50% sensitive. Conclusions: The steadily rising resistance in Pseudomonas species againstavailable antibiotics optionsnecessitates their use inlife-threatening and hospital acquiredinfections. Keywords: Peudomonas, Flouroquinolones, Carbapenem
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10

Park, Yoon-Dong, Hana Yi, Keun Sik Baik, Chi Nam Seong, Kyung Sook Bae, Eun Young Moon, and Jongsik Chun. "Pseudomonas segetis sp. nov., isolated from soil." International Journal of Systematic and Evolutionary Microbiology 56, no. 11 (November 1, 2006): 2593–95. http://dx.doi.org/10.1099/ijs.0.63792-0.

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A Gram-negative, aerobic bacterium, designated strain FR1439T, was isolated from the soil of Dokdo in the Republic of Korea. The cells of strain FR1439T were catalase- and oxidase-positive, motile and rod-shaped. Phylogenetic analysis of the 16S rRNA gene sequence revealed that it represents a distinct line of descent within the genus Pseudomonas. The levels of DNA–DNA relatedness between strain FR1439T and type strains of phylogenetically related species, namely Pseudomonas flavescens, Pseudomonas mendocina, Pseudomonas pseudoalcaligenes and Pseudomonas straminea, ranged from 28 to 37 %. Several phenotypic characteristics, together with the cellular fatty acid composition, can be used to differentiate strain FR1439T from related pseudomonads. On the basis of the polyphasic taxonomic evidence presented in this study, strain FR1439T represents a novel species, for which the name Pseudomonas segetis sp. nov. is proposed. The type strain is FR1439T (=IMSNU 14101T=CIP 108523T=KCTC 12331T).
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11

Behrendt, Undine, Andreas Ulrich, Peter Schumann, Jean-Marie Meyer, and Cathrin Spröer. "Pseudomonas lurida sp. nov., a fluorescent species associated with the phyllosphere of grasses." International Journal of Systematic and Evolutionary Microbiology 57, no. 5 (May 1, 2007): 979–85. http://dx.doi.org/10.1099/ijs.0.64793-0.

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The taxonomic position of a group of fluorescent pseudomonad strains isolated from the phyllosphere of grasses was investigated through a polyphasic approach. Riboprinting analysis revealed highly similar patterns for the investigated strains which supported, together with the agreement of many phenotypic characteristics, their affiliation to the same species. A comparison of 16S rRNA gene sequences of strain P 513/18T, a representative strain from the grass isolates, revealed that it was affiliated to the cluster of the ‘Pseudomonas fluorescens group’, with Pseudomonas costantinii as the closest phylogenetic neighbour. However, DNA–DNA hybridization showed a clear demarcation at the species level between strain P 513/18T and P. costantinii. Furthermore, a comparison of riboprint patterns with Pseudomonas species clustering next to the novel grass isolates on the basis of 16S rRNA gene sequences supported their separate species status at the phylogenetic level. Based on phenotypic features, the novel isolates could also be differentiated from the other fluorescent Pseudomonas species that share positive arginine dihydrolase and oxidase reactions. As a consequence of these phenotypic and phylogenetic analyses, the isolates from the grass pyllosphere represent a novel species for which the name Pseudomonas lurida sp. nov. is proposed. The type strain is P 513/18T (=DSM 15835T=LMG 21995T).
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12

Kim, Hye-Eun, Maiko Shitashiro, Akio Kuroda, Noboru Takiguchi, and Junichi Kato. "Ethylene Chemotaxis in Pseudomonas aeruginosa and Other Pseudomonas Species." Microbes and Environments 22, no. 2 (2007): 186–89. http://dx.doi.org/10.1264/jsme2.22.186.

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13

Prince, Alice. "Antibiotic resistance of Pseudomonas species." Journal of Pediatrics 108, no. 5 (May 1986): 830–34. http://dx.doi.org/10.1016/s0022-3476(86)80753-3.

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14

Garcés, J. L. Gómez, J. M. Aguado, M. A. Fernández-Clúa, and F. Soriano. "Pseudomonas species Ve-2 septicemia." European Journal of Clinical Microbiology 5, no. 2 (April 1986): 168–69. http://dx.doi.org/10.1007/bf02013978.

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15

Rathore, Pragya. "Role of Pseudomonas Species in Bioremediation of Contaminated Soil." Global Journal For Research Analysis 3, no. 6 (June 15, 2012): 197–98. http://dx.doi.org/10.15373/22778160/june2014/68.

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16

Zechman, James M., and John N. Labows Jr. "Volatiles of Pseudomonas aeruginosa and related species by automated headspace concentration – gas chromatography." Canadian Journal of Microbiology 31, no. 3 (March 1, 1985): 232–37. http://dx.doi.org/10.1139/m85-045.

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The volatile metabolites of three strains of Pseudomonas aeruginosa and one strain each of Pseudomonas cepacia, Pseudomonas maltophilia, Pseudomonas fluorescens, and Pseudomonas putida were analyzed using an automated headspace concentrator incorporating a gas chromatograph. The procedure does not require sample preparation and automates the entire analytical sequence to yield reproducible profiles of volatile constituents. Gas chromatographic profiles of the volatile metabolites of each species were obtained using a 20-min concentration period and two fused silica capillary columns of different polarities. The production of headspace metabolites from trypticase soy broth was studied in relationship to culture incubation time and initial cell concentration. The volatiles identified after 24 h incubation consisted of 1-butanol, isopentanol, toluene, 1-undecene, 2-butanone, 2-heptanone, 2-nonanone, and 2-undecanone. Sufficient amounts of specific metabolites were produced after 5 h incubation to provide information of possible diagnostic value. In particular, all P. aeruginosa strains produced a distinctive series of 1-undecene and methyl ketones after 5 h incubation of media inoculated to provide 2 × 106 cells/mL. The results indicate that when growth and analytical conditions are held constant, P. aeruginosa and related species produce characteristic profiles of headspace metabolites. Since conventional bacteriological tests require 24 h or more for the identification of these pseudomonads, automated volatile analysis could provide an alternative means for the rapid detection of these bacteria.
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17

Hoffmann, Lena, Michael-Frederick Sugue, and Thomas Brüser. "A tunable anthranilate-inducible gene expression system for Pseudomonas species." Applied Microbiology and Biotechnology 105, no. 1 (December 3, 2020): 247–58. http://dx.doi.org/10.1007/s00253-020-11034-8.

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Abstract Pseudomonads are among the most common bacteria in soils, limnic ecosystems, and human, animal, or plant host environments, including intensively studied species such as Pseudomonas aeruginosa, P. putida, or P. fluorescens. Various gene expression systems are established for some species, but there is still a need for a simple system that is suitable for a wide range of pseudomonads and that can be used for physiological applications, i.e., with a tuning capacity at lower expression levels. Here, we report the establishment of the anthranilate-dependent PantA promoter for tunable gene expression in pseudomonads. During studies on P. fluorescens, we constructed an anthranilate-inducible AntR/PantA-based expression system, named pUCP20-ANT, and used GFP as reporter to analyze gene expression. This system was compared with the rhamnose-inducible RhaSR/PrhaB-based expression system in an otherwise identical vector background. While the rhamnose-inducible system did not respond to lower inducer concentrations and always reached high levels over time when induced, expression levels of the pUCP20-ANT system could be adjusted to a range of distinct lower or higher levels by variation of anthranilate concentrations in the medium. Importantly, the anthranilate-inducible expression system worked also in strains of P. aeruginosa and P. putida and therefore will be most likely useful for physiological and biotechnological purposes in a wide range of pseudomonads. Key points • We established an anthranilate-inducible gene expression system for pseudomonads. • This system permits tuning of gene expression in a wide range of pseudomonads. • It will be very useful for physiological and biotechnological applications.
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18

Bophela, Khumbuzile N., Yolanda Petersen, Carolee T. Bull, and Teresa A. Coutinho. "Identification of Pseudomonas Isolates Associated With Bacterial Canker of Stone Fruit Trees in the Western Cape, South Africa." Plant Disease 104, no. 3 (March 2020): 882–92. http://dx.doi.org/10.1094/pdis-05-19-1102-re.

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Bacterial canker is a common bacterial disease of stone fruit trees. The causal agents responsible for the disease include several pathovars in Pseudomonas syringae sensu lato and newly described Pseudomonas species. Pseudomonad strains were isolated from symptomatic stone fruit trees, namely apricot, peach, and plum trees cultivated in spatially separated orchards in the Western Cape. A polyphasic approach was used to identify and characterize these strains. Using a multilocus sequence typing approach of four housekeeping loci, namely cts, gapA, gyrB, and rpoD, the pseudomonad strains were delineated into two phylogenetic groups within P. syringae sensu lato: P. syringae sensu stricto and Pseudomonas viridiflava. These results were further supported by LOPAT diagnostic assays and analysis of clades in the rep-PCR dendrogram. The pseudomonad strains were pathogenic on both apricot and plum seedlings, indicative of a lack of host specificity between Pseudomonas strains infecting Prunus spp. This is a first report of P. viridiflava isolated from plum trees showing symptoms of bacterial canker. P. viridiflava is considered to be an opportunistic pathogen that causes foliar diseases of vegetable crops, fruit trees, and aromatic herbs, and thus the isolation of pathogenic P. viridiflava from twigs of plum trees showing symptoms of bacterial canker suggests that this bacterial species is a potentially emerging stem canker pathogen of stone fruit trees in South Africa.
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Lüneberg, Edeltraud, Dirk Müller, Ivo Steinmetz, and Matthias Frosch. "Monoclonal antibody against species-specific epitope of Pseudomonas aeruginosa Hsp60 protein cross-reacts with Pseudomonas stutzeri and other Pseudomonas species." FEMS Microbiology Letters 154, no. 1 (January 17, 2006): 131–37. http://dx.doi.org/10.1111/j.1574-6968.1997.tb12634.x.

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Lüneberg, E. "Monoclonal antibody against species-specific epitope of Pseudomonas aeruginosa Hsp60 protein cross-reacts with Pseudomonas stutzeri and other Pseudomonas species." FEMS Microbiology Letters 154, no. 1 (September 1, 1997): 131–37. http://dx.doi.org/10.1016/s0378-1097(97)00313-3.

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21

Bozal, Núria, M. Jesús Montes, and Elena Mercadé. "Pseudomonas guineae sp. nov., a novel psychrotolerant bacterium from an Antarctic environment." International Journal of Systematic and Evolutionary Microbiology 57, no. 11 (November 1, 2007): 2609–12. http://dx.doi.org/10.1099/ijs.0.65141-0.

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Two Gram-negative, cold-adapted, aerobic bacteria, designated strains M8T and M6, were isolated from soil collected from the South Shetland Islands. The organisms were rod-shaped, catalase- and oxidase-positive and motile by means of polar flagella. These two psychrotolerant strains grew between −4 and 30 °C. 16S rRNA gene sequence analysis placed strains M8T and M6 within the genus Pseudomonas. DNA–DNA hybridization experiments between the Antarctic isolate M8T and type strains of phylogenetically related species, namely Pseudomonas peli and Pseudomonas anguilliseptica, revealed levels of relatedness of 33 and 37 %, respectively. Strain M6 showed 99 % DNA similarity to strain M8T. Several phenotypic characteristics, together with data on cellular fatty acid composition, served to differentiate strains M8T and M6 from related pseudomonads. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains M8T and M6 belong to the same genospecies, representing a novel species of the genus Pseudomonas, for which the name Pseudomonas guineae sp. nov. is proposed. The type strain is M8T (=LMG 24016T=CECT 7231T).
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22

Baltrus, David A., Kevin Dougherty, Beatriz Diaz, and Rachel Murillo. "Evolutionary Plasticity of AmrZ Regulation in Pseudomonas." mSphere 3, no. 2 (April 18, 2018): e00132-18. http://dx.doi.org/10.1128/msphere.00132-18.

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ABSTRACT amrZ encodes a master regulator protein conserved across pseudomonads, which can be either a positive or negative regulator of swimming motility depending on the species examined. To better understand plasticity in the regulatory function of AmrZ, we characterized the mode of regulation for this protein for two different motility-related phenotypes in Pseudomonas stutzeri. As in Pseudomonas syringae, AmrZ functions as a positive regulator of swimming motility within P. stutzeri, which suggests that the functions of this protein with regard to swimming motility have switched at least twice across pseudomonads. Shifts in mode of regulation cannot be explained by changes in AmrZ sequence alone. We further show that AmrZ acts as a positive regulator of colony spreading within this strain and that this regulation is at least partially independent of swimming motility. Closer investigation of mechanistic shifts in dual-function regulators like AmrZ could provide unique insights into how transcriptional pathways are rewired between closely related species. IMPORTANCE Microbes often display finely tuned patterns of gene regulation across different environments, with major regulatory changes controlled by a small group of “master” regulators within each cell. AmrZ is a master regulator of gene expression across pseudomonads and can be either a positive or negative regulator for a variety of pathways depending on the strain and genomic context. Here, we demonstrate that the phenotypic outcomes of regulation of swimming motility by AmrZ have switched at least twice independently in pseudomonads, so that AmrZ promotes increased swimming motility in P. stutzeri and P. syringae but represses this phenotype in Pseudomonas fluorescens and Pseudomonas aeruginosa. Since examples of switches in regulatory mode are relatively rare, further investigation into the mechanisms underlying shifts in regulator function for AmrZ could provide unique insights into the evolution of bacterial regulatory proteins.
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Meyer, Jean-Marie, Christelle Gruffaz, Topi Tulkki, and Daniel Izard. "Taxonomic heterogeneity, as shown by siderotyping, of strains primarily identified as Pseudomonas putida." International Journal of Systematic and Evolutionary Microbiology 57, no. 11 (November 1, 2007): 2543–56. http://dx.doi.org/10.1099/ijs.0.65233-0.

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One hundred and forty-four fluorescent pseudomonad strains isolated from various environments (soil, water, plant rhizosphere, hospital) and received as Pseudomonas putida (83 strains), P. putida biovar A (49 strains), P. putida biovar B (10 strains) and P. putida biovar C (2 strains), were analysed by the pyoverdine-isoelectrofocusing and pyoverdine-mediated iron uptake methods of siderotyping. Both methods demonstrated a great diversity among these strains, which could be subdivided into 35 siderovars. Some siderovars specifically included strains that have subsequently been transferred to well-defined Pseudomonas species, e.g. Pseudomonas monteilii or Pseudomonas mosselii, or which could be related by their siderotype to Pseudomonas jessenii or Pseudomonas mandelii. Other siderovars included strains sharing a high level of DNA-DNA relatedness (>70 %), thus demonstrating that siderotyping could easily circumscribe strains at the species level. However, a group of seven strains, including the type strain, P. putida ATCC 12633T, were allocated into four siderovars, despite sharing DNA–DNA relatedness values of higher than 70 %. Interestingly, the strong genomic relationships between these seven strains were supported by the structural relationships among their pyoverdines, thus reflecting their phylogenetic affinities. These results strongly support the view that pyoverdine-based siderotyping could be used as a powerful tool in Pseudomonas taxonomy.
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Tohya, Mari, Shin Watanabe, Tatsuya Tada, Htay Htay Tin, and Teruo Kirikae. "Genome analysis-based reclassification of Pseudomonas fuscovaginae and Pseudomonas shirazica as later heterotypic synonyms of Pseudomonas asplenii and Pseudomonas asiatica, respectively." International Journal of Systematic and Evolutionary Microbiology 70, no. 5 (May 1, 2020): 3547–52. http://dx.doi.org/10.1099/ijsem.0.004199.

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This study was conducted to clarify the taxonomic status of the species Pseudomonas fuscovaginae and Pseudomonas shirazica . Whole genome sequences for the type strains of P. fuscovaginae and P. shirazica were compared against the closely related type strains of the Pseudomonas putida group and the Pseudomonas fluorescens group species. Average nucleotide identity and digital DNA–DNA hybridization values between P. fuscovaginae LMG 2158T and Pseudomonas asplenii ATCC 23835T were 98.4 and 85.5 %, and between P. shirazica VM14T and Pseudomonas asiatica RYU5T were 99.3 and 95.3 %. These values were greater than recognized thresholds for bacterial species delineation, indicating that they belong to the same genomospecies, respectively. Therefore, P. fuscovaginae and P. shirazica should be reclassified as later heterotypic synonyms of P. asplenii and P. asiatica , respectively.
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25

Hameed, Asif, Mariyam Shahina, Shih-Yao Lin, You-Cheng Liu, and Chiu-Chung Young. "Pseudomonas hussainii sp. nov., isolated from droppings of a seashore bird, and emended descriptions of Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis." International Journal of Systematic and Evolutionary Microbiology 64, Pt_7 (July 1, 2014): 2330–37. http://dx.doi.org/10.1099/ijs.0.060319-0.

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Two Gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strains that are motile by a monopolar flagellum, designated CC-AMH-11T and CC-AMHZ-5, were isolated from droppings of a seashore bird off the coast of Hualien, Taiwan. The strains showed 99.7 % mutual pairwise 16S rRNA gene sequence similarity, while exhibiting <96.2 % sequence similarity to strains of other species of the genus Pseudomonas (95.7–95.9 % similarity with type species, Pseudomonas aeruginosa LMG 1242T), and formed a distinct co-phyletic lineage in the phylogenetic trees. The common major fatty acids (>5 % of the total) were C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8), C16 : 1ω6c and/or C16 : 1ω7c (summed feature 3), C16 : 0 and C12 : 0. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine, an unidentified lipid and an unidentified phospholipid were detected as common polar lipids. The DNA G+C contents of strains CC-AMH-11T and CC-AMHZ-5 were 61.1 and 61.6 mol%, respectively. The common major respiratory quinone was ubiquinone 9 (Q-9), and the predominant polyamine was putrescine. The DNA–DNA hybridization obtained between the two strains was 79.0 % (reciprocal value 89.4 % using CC-AMHZ-5 DNA as the probe). The very high 16S rRNA gene sequence similarity and DNA–DNA relatedness and the poorly distinguishable phenotypic features witnessed between CC-AMH-11T and CC-AMHZ-5 suggested unambiguously that they are two distinct strains of a single genomic species. However, the strains also showed several genotypic and phenotypic characteristics that distinguished them from other closely related species of Pseudomonas . Thus, the strains are proposed to represent a novel species of Pseudomonas , for which the name Pseudomonas hussainii sp. nov. is proposed. The type strain is CC-AMH-11T ( = JCM 19513T = BCRC 80696T); a second strain of the same species is CC-AMHZ-5 ( = JCM 19512 = BCRC 80697). In addition, emended descriptions of the species Pseudomonas pohangensis , Pseudomonas benzenivorans and Pseudomonas segetis are also proposed.
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Ullah, Saadat, Ijaz Malook, Khair Ul Bashar, Mehvish Riaz, Muhammad Mudasar Aslam, Zia Ur Rehman, Irfan Malook, Muhammad Fayyaz, and Muhammad Jamil. "Purification and Application of Lipases from Pseudomonas Species." Biological Sciences - PJSIR 59, no. 2 (August 24, 2016): 111–16. http://dx.doi.org/10.52763/pjsir.biol.sci.59.2.2016.111.116.

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Lipases are important hydrolytic enzymes that hydrolyze long chain triacylglycerol intodiacylglycerol, monoacylglycerol, glycerol and fatty acids. Lipases are found in microorganisms, fungi,plants and animals. Commercially, useful extracellular lipases are isolated from different bacterial species,including Bacillus, Achromobacter, Alcaligenes, Arthrobacter, Pseudomonas, Staphylococcus andChromobacterium species. Among the Pseudomonas species, Pseudomonas aeruginosa, P. cepacia andP. fluorescence are the major producers of lipases. Bacterial lipases have great industrial applicationsbecause of their stability, selectivity and broad substrate specificity. Due to their large scale applicationin industrial sectors, attention is given to isolate Pseudomonas lipases. In this review, purification strategiesfor lipases isolated from Pseudomonas species have been focussed.
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27

Bultreys, Alain, Isabelle Gheysen, Mathias Schäfer, Herbert Budzikiewicz, and Bernard Wathelet. "The Pyoverdins of Pseudomonas syringae and Pseudomonas cichorii." Zeitschrift für Naturforschung C 59, no. 9-10 (October 1, 2004): 613–18. http://dx.doi.org/10.1515/znc-2004-9-1001.

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Abstract The structure elucidation of the cyclic (lactonic) forms of the pyoverdins with a succinamide side chain originally produced by the closely related species Pseudomonas syringae and P. cichorii is reported. Mass spectrometry and nuclear magnetic resonance analyses as well as the determination of the configuration of the amino acids after degradation indicate that these two pyoverdins differ only by the replacement of the first in-chain serine by glycine. The pyoverdins of P. syringae and P. cichorii and the dihydropyoverdin of P. syringae can be used by both species as siderophores.
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28

S. Joshi, Abhijeet, and M. P. Chitanand. "Metabolic Diversity of Rhizospheric Pseudomonas species of Bt Cotton Plant." Journal of Pure and Applied Microbiology 12, no. 4 (December 30, 2018): 1929–37. http://dx.doi.org/10.22207/jpam.12.4.29.

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29

Aprameya, IndumathiVrithamani. "Non-fermenters other than Pseudomonas species." Journal of The Academy of Clinical Microbiologists 15, no. 2 (2013): 62. http://dx.doi.org/10.4103/0972-1282.124589.

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30

George, Susan E., and Larry D. Claxton. "Biotransformation of chlordecone by Pseudomonas species." Xenobiotica 18, no. 4 (January 1988): 407–16. http://dx.doi.org/10.3109/00498258809041677.

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31

Wilson, R., and R. B. Dowling. "Pseudomonas aeruginosa and other related species." Thorax 53, no. 3 (March 1, 1998): 213–19. http://dx.doi.org/10.1136/thx.53.3.213.

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32

JOHNSON, J. L., and N. J. PALLERONI. "Deoxyribonucleic Acid Similarities among Pseudomonas Species." International Journal of Systematic Bacteriology 39, no. 3 (July 1, 1989): 230–35. http://dx.doi.org/10.1099/00207713-39-3-230.

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33

Chan, Yiu-Kwok. "Denitrification by a diazotrophic Pseudomonas species." Canadian Journal of Microbiology 31, no. 12 (December 1, 1985): 1136–41. http://dx.doi.org/10.1139/m85-214.

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Pseudomonas sp. type strain H8 is a N2-fixing H2-utilizing bacterium frequently isolated from the root of wetland rice. It was previously reported not to denitrify although [Formula: see text] was reduced to [Formula: see text]. In the present study it grew anaerobically with [Formula: see text], [Formula: see text], or N2O as electron acceptor. Its capability to denitrify was confirmed by using the C2H2-inhibition technique in both undefined and defined media with glucose as carbon source. Chemolithotrophic denitrification of [Formula: see text] with H2 and CO2 was also demonstrated. Its H2-uptake activity was found to be higher when grown initially in the presence of 10% O2 than 21% O2 or 5 mM[Formula: see text] Compared with Azospirillum brasilense, a N2-fixing bacterium capable of denitrification, H8 was not sensitive to [Formula: see text] toxicity under similar experimental conditions. Preliminary evidence suggested that N2O reduction by H8, but not by A. brasilense, was inhibited by yeast extract. The denitrification capability of H8 may prove to be an undesirable characteristic that leads to the removal of [Formula: see text] N from soils.
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34

Kim, Hyunhee, Seongjoon Moon, Soojeong Ham, Kihyun Lee, Ute Römling, and Changhan Lee. "Cytoplasmic molecular chaperones in Pseudomonas species." Journal of Microbiology 60, no. 11 (November 1, 2022): 1049–60. http://dx.doi.org/10.1007/s12275-022-2425-0.

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35

Sazinas, Pavelas, Morten Lindqvist Hansen, May Iren Aune, Marie Højmark Fischer, and Lars Jelsbak. "A Rare Thioquinolobactin Siderophore Present in a Bioactive Pseudomonas sp. DTU12.1." Genome Biology and Evolution 11, no. 12 (December 1, 2019): 3529–33. http://dx.doi.org/10.1093/gbe/evz267.

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Abstract Many of the soil-dwelling Pseudomonas species are known to produce secondary metabolite compounds, which can have antagonistic activity against other microorganisms, including important plant pathogens. It is thus of importance to isolate new strains of Pseudomonas and discover novel or rare gene clusters encoding bioactive products. In an effort to accomplish this, we have isolated a bioactive Pseudomonas strain DTU12.1 from leaf-covered soil in Denmark. Following genome sequencing with Illumina and Oxford Nanopore technologies, we generated a complete genome sequence with the length of 5,943,629 base pairs. The DTU12.1 strain contained a complete gene cluster for a rare thioquinolobactin siderophore, which was previously described as possessing bioactivity against oomycetes and several fungal species. We placed the DTU12.1 strain within Pseudomonas gessardii subgroup of fluorescent pseudomonads, where it formed a distinct clade with other Pseudomonas strains, most of which also contained a complete thioquinolobactin gene cluster. Only two other Pseudomonas strains were found to contain the gene cluster, though they were present in a different phylogenetic clade and were missing a transcriptional regulator of the whole cluster. We show that having the complete genome sequence and establishing phylogenetic relationships with other strains can enable us to start evaluating the distribution and evolutionary origins of secondary metabolite clusters.
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36

Mortensen, Joel E., Margaret C. Fisher, and John J. LiPuma. "Recovery of Pseudomonas cepacia and Other Pseudomonas Species from the Environment." Infection Control and Hospital Epidemiology 16, no. 1 (January 1995): 30–32. http://dx.doi.org/10.2307/30140998.

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37

Mortensen, Joel E., Margaret C. Fisher, and John J. LiPuma. "Recovery of Pseudomonas cepacia and Other Pseudomonas Species from the Environment." Infection Control and Hospital Epidemiology 16, no. 1 (January 1995): 30–32. http://dx.doi.org/10.1086/646999.

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38

Foght, J. M., and D. W. S. Westlake. "Degradation of polycyclic aromatic hydrocarbons and aromatic heterocycles by a Pseudomonas species." Canadian Journal of Microbiology 34, no. 10 (October 1, 1988): 1135–41. http://dx.doi.org/10.1139/m88-200.

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Enrichment cultures were established with the aromatic fraction of a crude oil and screened for aromatic-degrading pseudomonads, using a sprayed plate technique. One isolate identified as Pseudomonas sp. HL7b was chosen for further study because it oxidized several polycyclic aromatic hydrocarbons and aromatic heterocycles without an apparent lag. Using capillary gas chromatography, spectrophotometry, and radiorespirometry, it was found to be capable of mineralizing and (or) oxidizing a wide range of polycyclic aromatic hydrocarbons, S-, N-, and O-heterocyclic analogues, and alkyl polycyclic aromatic hydrocarbons, but not aliphatic hydrocarbons. The isolate displayed two colonial morphologies which correlated with variation in degradative phenotype and hydrophobicity as measured by polystyrene adherence. Four cryptic plasmids were observed in both colonial types. Pseudomonas sp. HL7b degraded dibenzothiophene co-metabolically by a recognized pathway, but this degradation was constitutive, rather than inducible as reported for other bacteria.
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39

Doszhanov, Ye O., Ye K. Ongarbaev, Z. A. Mansurov, A. A. Zhubanova, and Martin Hofrichter. "Bioremedation of Oil and Oil Products Bacterial Species of the Genus Pseudomonas." Eurasian Chemico-Technological Journal 12, no. 2 (January 11, 2010): 157. http://dx.doi.org/10.18321/ectj39.

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In the article the results of investigation of oxidation processes of crude-oil by bacteria of genus Pseudomonas: Pseudomonas mendocina H-3, Pseudomonas sp. H-7, Pseudomonas stutzeri H-10, Pseudomonas aeruginosa H-14, Pseudomonas alcaligenes H-15 and Pseudomonas sp H-16. It was shown that these microorganisms are capable to oxidize oil’s hydrocarbons and oil products. It means that the bacteria may be used for bioremediation of oil-pollutant soils.
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40

Elting, Linda S., and Gerald P. Bodey. "Septicemia Due to Xanthomonas Species and Non-Aeruginosa Pseudomonas Species." Medicine 69, no. 5 (September 1990): 296–306. http://dx.doi.org/10.1097/00005792-199009000-00003.

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41

Stevens, Joanne, Barry L. Fanburg, and Joseph J. Lanzillo. "Determination of peptidyl dipeptidase activity in 24 bacterial species." Canadian Journal of Microbiology 36, no. 1 (January 1, 1990): 56–59. http://dx.doi.org/10.1139/m90-010.

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Of 24 bacterial species examined for lisinopril refractive peptidyl dipeptidase activity, only 8 contained activity. Activity in Pseudomonas maltophilia was more than fourfold higher than that of any other species. Pseudomonas maltophilia may be unique among bacteria in possessing high peptidyl dipeptidase activity that is both EDTA inhibitable and lisinopril resistant. Key words: Pseudomonas maltophilia, peptidyl dipeptidase.
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42

Bueno-Gonzalez, Victoria, Carrie Brady, Sandra Denman, Joël Allainguillaume, and Dawn Arnold. "Pseudomonas kirkiae sp. nov., a novel species isolated from oak in the United Kingdom, and phylogenetic considerations of the genera Pseudomonas, Azotobacter and Azomonas." International Journal of Systematic and Evolutionary Microbiology 70, no. 4 (April 1, 2020): 2426–34. http://dx.doi.org/10.1099/ijsem.0.004055.

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As the current episode of Acute Oak Decline (AOD) continues to affect native British oak in the United Kingdom, ongoing isolations from symptomatic and healthy oak have yielded a large Pseudomonas species population. These strains could be divided into taxa representing three potential novel species. Recently, two of these taxa were described as novel Pseudomonas species in the Pseudomonas fluorescens lineage. Here, we demonstrate using a polyphasic approach that the third taxon represents another novel Pseudomonas species. The 16S rRNA gene sequencing assigned the strains to the Pseudomonas aeruginosa lineage, while multilocus sequence analysis (based on partial gyrB, rpoB and rpoD sequences) placed the 13 strains in a single cluster on the border of the Pseudomonas stutzeri group. Whole genome intra-species comparisons (based on average nucleotide identity and in silico DNA–DNA hybridization) confirmed that the strains belong to a single taxon, while the inter-species comparisons with closest phylogenetic relatives yielded similarity values below the accepted species threshold. Therefore, we propose these strains as a novel species, namely Pseudomonas kirkiae sp. nov., with the type strain FRB 229T (P4CT=LMG 31089T=NCPPB 4674T). The phylogenetic analyses performed in this study highlighted the difficulties in assigning novel species to the genus Pseudomonas due to its polyphyletic nature and close relationship to the genus Azotobacter . We further propose that a thorough taxonomic re-evaluation of the genus Pseudomonas is essential and should be performed in the near future.
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43

Bultreys, Alain, Isabelle Gheysen, Bernard Wathelet, Henri Maraite, and Edmond de Hoffmann. "High-Performance Liquid Chromatography Analyses of Pyoverdin Siderophores Differentiate among Phytopathogenic Fluorescent Pseudomonas Species." Applied and Environmental Microbiology 69, no. 2 (February 2003): 1143–53. http://dx.doi.org/10.1128/aem.69.2.1143-1153.2003.

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ABSTRACT The relationship of pyoverdins produced by 41 pathovars of Pseudomonas syringae and by phytopathogenic Pseudomonas species was investigated. A high-performance liquid chromatography method for analyzing the culture medium proved to be superior to isoelectric focusing for detecting pyoverdin production, for differentiating slightly different pyoverdins, and for differentiating atypical from typical Fe(III)-chelated pyoverdins. Nonfluorescent strains were found in Pseudomonas amygdali, Pseudomonas meliae, Pseudomonas fuscovaginae, and P. syringae. Pseudomonas agarici and Pseudomonas marginalis produced typical pyoverdins. Among the arginine dihydrolase-negative fluorescent Pseudomonas species, spectral, amino acid, and mass spectrometry analyses underscored for the first time the clear similarities among the pyoverdins produced by related species. Within this group, the oxidase-negative species Pseudomonas viridiflava and Pseudomonas ficuserectae and the pathovars of P. syringae produced the same atypical pyoverdin, whereas the oxidase-positive species Pseudomonas cichorii produced a similar atypical pyoverdin that contained a glycine instead of a serine. The more distantly related species Pseudomonas asplenii and Pseudomonas fuscovaginae both produced a less similar atypical pyoverdin. The spectral characteristics of Fe(III)-chelated atypical pyoverdins at pH 7.0 were related to the presence of two β-hydroxyaspartic acids as iron ligands, whereas in typical pyoverdins one of the ligands is always ornithine based. The peptide chain influenced the chelation of iron more in atypical pyoverdins. Our results demonstrated that there is relative pyoverdin conservation in the amino acids involved in iron chelation and that there is faster evolution of the other amino acids, highlighting the usefulness of pyoverdins in systematics and in identification.
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44

Girard, Léa, Cédric Lood, Monica Höfte, Peter Vandamme, Hassan Rokni-Zadeh, Vera van Noort, Rob Lavigne, and René De Mot. "The Ever-Expanding Pseudomonas Genus: Description of 43 New Species and Partition of the Pseudomonas putida Group." Microorganisms 9, no. 8 (August 18, 2021): 1766. http://dx.doi.org/10.3390/microorganisms9081766.

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The genus Pseudomonas hosts an extensive genetic diversity and is one of the largest genera among Gram-negative bacteria. Type strains of Pseudomonas are well known to represent only a small fraction of this diversity and the number of available Pseudomonas genome sequences is increasing rapidly. Consequently, new Pseudomonas species are regularly reported and the number of species within the genus is constantly evolving. In this study, whole genome sequencing enabled us to define 43 new Pseudomonas species and provide an update of the Pseudomonas evolutionary and taxonomic relationships. Phylogenies based on the rpoD gene and whole genome sequences, including, respectively, 316 and 313 type strains of Pseudomonas, revealed sixteen groups of Pseudomonas and, together with the distribution of cyclic lipopeptide biosynthesis gene clusters, enabled the partitioning of the P. putida group into fifteen subgroups. Pairwise average nucleotide identities were calculated between type strains and a selection of 60 genomes of non-type strains of Pseudomonas. Forty-one strains were incorrectly assigned at the species level and among these, 19 strains were shown to represent an additional 13 new Pseudomonas species that remain to be formally classified. This work pinpoints the importance of correct taxonomic assignment and phylogenetic classification in order to perform integrative studies linking genetic diversity, lifestyle, and metabolic potential of Pseudomonas spp.
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45

Syed Sajeed Ali, Syed Sajeed Ali. "FAME Analysis for Identification of Clinical Isolate Siderophore Producing Pseudomonas Species." Indian Journal of Applied Research 3, no. 11 (October 1, 2011): 424–26. http://dx.doi.org/10.15373/2249555x/nov2013/135.

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46

Dubuis, Christophe, and Dieter Haas. "Cross-Species GacA-Controlled Induction of Antibiosis in Pseudomonads." Applied and Environmental Microbiology 73, no. 2 (November 10, 2006): 650–54. http://dx.doi.org/10.1128/aem.01681-06.

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ABSTRACT Signal extracts prepared from culture supernatants of Pseudomonas fluorescens CHA0 and Pseudomonas aeruginosa PAO stimulated GacA-dependent expression of small RNAs and hence of antibiotic compounds in both hosts. Pseudomonas corrugata LMG2172 and P. fluorescens SBW25 also produced signal molecules stimulating GacA-controlled antibiotic synthesis in strain CHA0, illustrating a novel, N-acyl-homoserine lactone-independent type of interspecies communication.
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47

Dutta, Bhabesh, Ronald Gitaitis, Gaurav Agarwal, Teresa Coutinho, and David Langston. "Pseudomonas coronafaciens sp. nov., a new phytobacterial species diverse from Pseudomonas syringae." PLOS ONE 13, no. 12 (December 6, 2018): e0208271. http://dx.doi.org/10.1371/journal.pone.0208271.

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48

Cabrera, Ma Ángeles, Sebastián L. Márquez, and José M. Pérez-Donoso. "Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation." Genes 13, no. 8 (July 28, 2022): 1354. http://dx.doi.org/10.3390/genes13081354.

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The nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. Since physicochemical methods for remediation are poorly effective, the use of microorganisms has gained interest as an alternative to restore TNT-contaminated sites. We previously demonstrated the high TNT-transforming capability of three novel Pseudomonas spp. isolated from Deception Island, Antarctica, which exceeded that of the well-characterized TNT-degrading bacterium Pseudomonas putida KT2440. In this study, a comparative genomic analysis was performed to search for the metabolic functions encoded in the genomes of these isolates that might explain their TNT-transforming phenotype, and also to look for differences with 21 other selected pseudomonads, including xenobiotics-degrading species. Comparative analysis of xenobiotic degradation pathways revealed that our isolates have the highest abundance of key enzymes related to the degradation of fluorobenzoate, TNT, and bisphenol A. Further comparisons considering only TNT-transforming pseudomonads revealed the presence of unique genes in these isolates that would likely participate directly in TNT-transformation, and others involved in the β-ketoadipate pathway for aromatic compound degradation. Lastly, the phylogenomic analysis suggested that these Antarctic isolates likely represent novel species of the genus Pseudomonas, which emphasizes their relevance as potential agents for the bioremediation of TNT and other xenobiotics.
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49

Hester, Kathryn L., Jodi Lehman, Fares Najar, Lin Song, Bruce A. Roe, Carolyn H. MacGregor, Paul W. Hager, Paul V. Phibbs, and John R. Sokatch. "Crc Is Involved in Catabolite Repression Control of the bkd Operons of Pseudomonas putida andPseudomonas aeruginosa." Journal of Bacteriology 182, no. 4 (February 15, 2000): 1144–49. http://dx.doi.org/10.1128/jb.182.4.1144-1149.2000.

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ABSTRACT Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study, the role of Crc in catabolite repression control has been studied in Pseudomonas putida. The bkd operons of P. putida and P. aeruginosa encode the inducible multienzyme complex branched-chain keto acid dehydrogenase, which is regulated in both species by catabolite repression. We report here that this effect is mediated in both species by Crc. A 13-kb cloned DNA fragment containing the P. putida crc gene region was sequenced. Crc regulates the expression of branched-chain keto acid dehydrogenase, glucose-6-phosphate dehydrogenase, and amidase in both species but not urocanase, although the carbon sources responsible for catabolite repression in the two species differ. Transposon mutants affected in their expression of BkdR, the transcriptional activator of thebkd operon, were isolated and identified as crcand vacB (rnr) mutants. These mutants suggested that catabolite repression in pseudomonads might, in part, involve control of BkdR levels.
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Maier, Christopher, Katharina Hofmann, Christopher Huptas, Siegfried Scherer, Mareike Wenning, and Genia Lücking. "Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR." Applied Microbiology and Biotechnology 105, no. 4 (February 2021): 1693–708. http://dx.doi.org/10.1007/s00253-021-11109-0.

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Abstract The heat-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in raw milk, is a major cause of destabilization and premature spoilage of ultra-high temperature (UHT) milk and milk products. To enable rapid detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in raw milk, we developed two triplex qPCR assays taking into account species-dependent differences in AprX activity. Besides five species-specific hydrolysis probes, targeting the aprX gene, a universal rpoB probe was included in the assay to determine the total Pseudomonas counts. For all six probes, linear regression lines between Cq value and target DNA concentration were obtained in singleplex as well as in multiplex approaches, yielding R2 values of > 0.975 and amplification efficiencies of 85–97%. Moreover, high specificity was determined using genomic DNA of 75 Pseudomonas strains, assigned to 57 species, and 40 other bacterial species as templates in the qPCR. Quantification of the target species and total Pseudomonas counts resulted in linear detection ranges of approx. 103–107 cfu/ml, which correspond well to common Pseudomonas counts in raw milk. Application of the assay using 60 raw milk samples from different dairies showed good agreement of total Pseudomonas counts calculated by qPCR with cell counts derived from cultivation. Furthermore, a remarkably high variability regarding the species composition was observed for each milk sample, whereby P. lundensis and P. proteolytica/P. gessardii were the predominant species detected. Key points • Multiplex qPCR for quantification of seven proteolytic Pseudomonas species and total Pseudomonas counts in raw milk • High specificity and sensitivity via hydrolysis probes against aprX and rpoB • Rapid method to determine Pseudomonas contamination in raw milk and predict spoilage potential
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