Relevant bibliographies by topics / Pseudomonas savastanoi

Academic literature on the topic 'Pseudomonas savastanoi'

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Published: 10 March 2023

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Journal articles on the topic "Pseudomonas savastanoi"

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Pérez-Martínez, Isabel, Youfu Zhao, Jesús Murillo, George W. Sundin, and Cayo Ramos. "Global Genomic Analysis of Pseudomonas savastanoi pv. savastanoi Plasmids." Journal of Bacteriology 190, no. 2 (November 9, 2007): 625–35. http://dx.doi.org/10.1128/jb.01067-07.

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ABSTRACT Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of information about the gene content of P. savastanoi pv. savastanoi plasmids and their role in the interaction of this pathogen with olive plants. We designed a DNA macroarray containing 135 plasmid-borne P. syringae genes to conduct a global genetic analysis of 32 plasmids obtained from 10 P. savastanoi pv. savastanoi strains. Hybridization results revealed that the number of PFPs per strain varied from one to four. Additionally, most strains contained at least one plasmid (designated non-PFP) that did not hybridize to the repA gene of pPT23A. Only three PFPs contained genes involved in the biosynthesis of the virulence factor indole-3-acetic acid (iaaM, iaaH, and iaaL). In contrast, ptz, a gene involved in the biosynthesis of cytokinins, was found in five PFPs and one non-PFP. Genes encoding a type IV secretion system (T4SS), type IVA, were found in both PFPs and non-PFPs; however, type IVB genes were found only on PFPs. Nine plasmids encoded both T4SSs, whereas seven other plasmids carried none of these genes. Most PFPs and non-PFPs hybridized to at least one putative type III secretion system effector gene and to a variety of additional genes encoding known P. syringae virulence factors and one or more insertion sequence transposase genes. These results indicate that non-PFPs may contribute to the virulence and fitness of the P. savastanoi pv. savastanoi host. The overall gene content of P. savastanoi pv. savastanoi plasmids, with their repeated information, mosaic arrangement, and insertion sequences, suggests a possible role in adaptation to a changing environment.
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Matas, Isabel M., Isabel P�rez-Mart�nez, Jos� M. Quesada, Jos� J. Rodr�guez-Herva, Ram�n Penyalver, and Cayo Ramos. "Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat." Applied and Environmental Microbiology 75, no. 4 (December 19, 2008): 1030–35. http://dx.doi.org/10.1128/aem.01572-08.

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ABSTRACT In this study, Pseudomonas savastanoi pv. savastanoi isolates were demonstrated to contain two iaaL paralogs, which are both chromosomally located in most strains. Comparative analysis of iaaL nucleotide sequences amplified from these two paralogs revealed that one paralog, iaaL Psn, is 100% identical to iaaL from P. savastanoi pv. nerii, while the other paralog, iaaL Psv, exhibited 93% identity to iaaL from Pseudomonas syringae pv. tomato (iaaL Pto). A 3-nucleotide motif (TAC) comprised of 3 to 15 repeats, which remained stable after propagation of the strains in olive plants, was found in iaaL Psv. Based on the observed nucleotide sequence variations, a restriction fragment length polymorphism assay was developed that allowed differentiation among iaaL Psn, iaaL Psv, and iaaL Pto . In addition, reverse transcriptase PCR on total RNA from P. savastanoi pv. savastanoi strains demonstrated that both iaaL Psv and iaaL Psn containing 14 or fewer TAC repeats are transcribed. Capillary electrophoresis analysis of PCR-amplified DNA fragments containing the TAC repeats from iaaL Psv allowed the differentiation of P. savastanoi pv. savastanoi isolates.
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Rodríguez-Moreno, Luis, Antonio J. Jiménez, and Cayo Ramos. "Endopathogenic lifestyle of Pseudomonas savastanoi pv. savastanoi in olive knots." Microbial Biotechnology 2, no. 4 (March 18, 2009): 476–88. http://dx.doi.org/10.1111/j.1751-7915.2009.00101.x.

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Penyalver, Ramón, Amparo García, Amparo Ferrer, Edson Bertolini, and María M. López. "Detection of Pseudomonas savastanoi pv. savastanoi in Olive Plants by Enrichment and PCR." Applied and Environmental Microbiology 66, no. 6 (June 1, 2000): 2673–77. http://dx.doi.org/10.1128/aem.66.6.2673-2677.2000.

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ABSTRACT The sequence of the gene iaaL of Pseudomonas savastanoi EW2009 was used to design primers for PCR amplification. The iaaL-derived primers directed the amplification of a 454-bp fragment from genomic DNA isolated from 70 strains of P. savastanoi, whereas genomic DNA from 93 non-P. savastanoi isolates did not yield this amplified product. A previous bacterial enrichment in the semiselective liquid medium PVF-1 improved the PCR sensitivity level, allowing detection of 10 to 100 CFU/ml of plant extract. P. savastanoi was detected by the developed enrichment-PCR method in knots from different varieties of inoculated and naturally infected olive trees. Moreover,P. savastanoi was detected in symptomless stem tissues from naturally infected olive plants. This enrichment-PCR method is more sensitive and less cumbersome than the conventional isolation methods for detection of P. savastanoi.
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P�rez-Mart�nez, Isabel, Luis Rodr�guez-Moreno, Lotte Lambertsen, Isabel M. Matas, Jes�s Murillo, Stefania Tegli, Antonio J. Jim�nez, and Cayo Ramos. "Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants (Olea europaea L.)." Applied and Environmental Microbiology 76, no. 11 (April 2, 2010): 3611–19. http://dx.doi.org/10.1128/aem.00133-10.

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ABSTRACT Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS-to-hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructed a mutant of NCPPB 3335, termed T3, which carries a deletion from the 3′ end of the hrpS gene to the 5′ end of the hrpZ operon. Despite its inability to multiply in olive tissues and to induce tumor formation in woody olive plants, P. savastanoi pv. savastanoi T3 can induce knot formation on young micropropagated olive plants. However, the necrosis and formation of internal open cavities previously reported in knots induced by the wild-type strain were not observed in those induced by P. savastanoi pv. savastanoi T3. Tagging of P. savastanoi pv. savastanoi T3 with green fluorescent protein (GFP) allowed real-time monitoring of its behavior on olive plants. In olive plant tissues, the wild-type strain formed aggregates that colonized the intercellular spaces and internal cavities of the hypertrophic knots, while the mutant T3 strain showed a disorganized distribution within the parenchyma of the knot. Ultrastructural analysis of knot sections revealed the release of extensive outer membrane vesicles from the bacterial cell surface of the P. savastanoi pv. savastanoi T3 mutant, while the wild-type strain exhibited very few vesicles. This phenomenon has not been described before for any other bacterial phytopathogen during host infection.
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Moreno-Pérez, Alba, Cayo Ramos, and Luis Rodríguez-Moreno. "HrpL Regulon of Bacterial Pathogen of Woody Host Pseudomonas savastanoi pv. savastanoi NCPPB 3335." Microorganisms 9, no. 7 (July 5, 2021): 1447. http://dx.doi.org/10.3390/microorganisms9071447.

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The Pseudomonas savastanoi species comprises a group of phytopathogenic bacteria that cause symptoms of disease in woody hosts. This is mediated by the rapid activation of a pool of virulence factors that suppress host defences and hijack the host’s metabolism to the pathogen’s benefit. The hrpL gene encodes an essential transcriptional regulator of virulence functions, including the type III secretion system (T3SS), in pathogenic bacteria. Here, we analyzed the contribution of HrpL to the virulence of four pathovars (pv.) of P. savastanoi isolated from different woody hosts (oleander, ash, broom, and dipladenia) and characterized the HrpL regulon of P. savastanoi pv. savastanoi NCPPB 3335 using two approaches: whole transcriptome sequencing (RNA-seq) and the bioinformatic prediction of candidate genes containing an hrp-box. Pathogenicity tests carried out for the P. savastanoi pvs. showed that HrpL was essential for symptom development in both non-host and host plants. The RNA-seq analysis of the HrpL regulon in P. savastanoi revealed a total of 53 deregulated genes, 49 of which were downregulated in the ΔhrpL mutant. Bioinformatic prediction resulted in the identification of 50 putative genes containing an hrp-box, 16 of which were shared with genes previously identified by RNA-seq. Although most of the genes regulated by HrpL belonged to the T3SS, we also identified some genes regulated by HrpL that could encode potential virulence factors in P. savastanoi.
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Martin, J. L., S. D. Comfort, P. J. Shea, R. A. Drijber, and T. A. Kokjohn. "Denitration of 2,4,6-trinitrotoluene byPseudomonas savastanoi." Canadian Journal of Microbiology 43, no. 5 (May 1, 1997): 447–55. http://dx.doi.org/10.1139/m97-063.

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Past disposal of wastewaters containing 2,4,6-trinitrotoluene (TNT) at the former Nebraska Ordnance Plant has resulted in numerous acres of TNT-contaminated soil. Examining the microbial population of these soils revealed several TNT-tolerant Pseudomonas spp. We selected one species, P. savastanoi, to determine its ability to transform TNT. Pure culture experiments were performed in pseudomonas minimal medium containing 0.31 mM TNT (70 mg TNT∙L−1) under varied nutrient and cell density regimes. Experiments with TNT as a sole C or N source showed that P. savastanoi has the ability to denitrate TNT, as evidenced by production of 2,4-dinitrotoluene (2,4-DNT) and NO2−with time. TNT denitration and formation of 2,4-DNT were enhanced by removing NH4+and adding NO2−to the growth medium. In all experiments, 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT) appeared as incidental reduction products. Glucose addition to the medium enhanced 2-ADNT and 4-ADNT production and decreased denitration of TNT. Mid-log phase cells rapidly transformed [ring-14C(U)]TNT but were unable to mineralize significant quantities of TNT, as evidenced by conversion of less than 1% of the label to14CO2. These results indicate that P. savastanoi is a TNT-tolerant pseudomonad that can promote TNT degradation through reductive denitration and nitro moiety reduction.Key words: TNT, biodegradation, transformation, reduction, nitrite.
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Rodríguez-Moreno, Luis, Araceli Barceló-Muñoz, and Cayo Ramos. "In Vitro Analysis of the Interaction of Pseudomonas savastanoi pvs. savastanoi and nerii with Micropropagated Olive Plants." Phytopathology® 98, no. 7 (July 2008): 815–22. http://dx.doi.org/10.1094/phyto-98-7-0815.

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This study assessed the use of in vitro olive plants to evaluate the virulence of Pseudomonas savastanoi pv. savastanoi strains isolated from olive and P. savastanoi pv. nerii strains isolated from oleander knots. First, different olive isolates were inoculated into stem wounds and differences in knot formation and weight of overgrowths were observed for the selected strains. Tissue proliferation was clearly visible in all inoculated plants 30 days after inoculation. Virulence of P. savastanoi pv. nerii mutants with defects in regard to biosynthesis of indole-3-acetic acid and/or cytokinins was tested using this system. In agreement with data previously reported, all mutant strains multiplied in olive but induced attenuated symptoms. To analyze the virulence of P. savastanoi pv. savastanoi affected in their ability to grow in olive tissue, a trpE tryptophan auxotroph mutant was generated using a collection of signature tagged mutagenesis transposons. Virulence of this mutant was clearly reduced as evidenced by swelling of the olive tissue that evolved into attenuated knots. Furthermore, mixed infections with its parental strain revealed that the wild-type strain completely out-competed the trpE mutant. Results shown here demonstrate the usefulness of in vitro olive plants for the analysis of P. savastanoi pvs. savastanoi and nerii virulence. In addition, this system offers the possibility of quantifying virulence differences as weight of overgrowths. Moreover, we established the basis for the use of mixed infections in combination with signature tagged mutagenesis for high-throughput functional genomic analysis of this bacterial pathogen.
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Marchi, G., A. Sisto, A. Cimmino, A. Andolfi, M. G. Cipriani, A. Evidente, and G. Surico. "Interaction between Pseudomonas savastanoi pv. savastanoi and Pantoea agglomerans in olive knots." Plant Pathology 55, no. 5 (October 2006): 614–24. http://dx.doi.org/10.1111/j.1365-3059.2006.01449.x.

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Quesada, Jose M., Isabel Pérez-Martínez, Cayo Ramos, María M. López, and Ramón Penyalver. "IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi." Research in Microbiology 159, no. 3 (April 2008): 207–15. http://dx.doi.org/10.1016/j.resmic.2007.12.010.

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Dissertations / Theses on the topic "Pseudomonas savastanoi"

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Abu-Ghorrah, Mahmoud. "Taxonomie et pouvoir pathogène de Pseudomonas syringae PV. Savastanoi." Grenoble 2 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb37611126s.

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Noble, Thomas J. "Molecular characterisation and identification of Pseudomonas savastanoi pv. Phaseolicola, infecting mungbeans in Australia." Thesis, Queensland University of Technology, 2020. https://eprints.qut.edu.au/205533/1/Thomas_Noble_Thesis.pdf.

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This research explores population genetics for the bacterial pathogen Pseudomonas savastanoi pv. phaseolicola, which causes halo blight disease in its host mungbeans (Vigna radiata). The pathogen and host populations were investigated at a board scale using field and glasshouse studies and in detail using molecular biology techniques including qPCR and next-generation sequencing. The study found both the bacterial pathogen and host (mungbean) to have highly conserved genetic backgrounds. This will make it easier for breeders to target critical resistance genes to prevent the infection of the halo blight pathogen in future cultivars.
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Ammouneh, Hassan. "Molecular characterisation of virulence genes on a pathogenicity island in Pseudomonas savastanoi pv. phaseolicola." Thesis, Imperial College London, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.405032.

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Correia, Petra Karina Morais. "Diversidade molecular em estirpes de Pseudomonas savastanoi isoladas de nódulos de Olea europaea L. portuguesa." Master's thesis, Universidade de Évora, 2007. http://hdl.handle.net/10174/16350.

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Pseudomonas savastanoi provoca a doença dos nódulos de oliveira. Neste estudo aplicou‐se uma abordagem polifásica na caracterização de isolados (produtores de IAA) obtidos a partir de nódulos de três variedades de oliveira. Aplicaram‐se testes fenotípicos clássicos e moleculares, nomeadamente para deteção do gene iaaL, PCR‐RFLPs do rDNA 16S e 'fingerprinting' genómico por BOX‐, ERIC‐ e REP‐PCR. Obteve‐se uma grande heterogeneidade fenética entre isolados identificados como P. savastanoi. Recorreu‐se à sequenciação do rDNA 168 e verificou‐se que apenas um isolado apresentava homologia com P. savastanoi, sendo os restantes homólogos de Pantoea agglomerans, Pantoea oleae e Erwinia toletana, comprovando a falta de fiabilidade dos outros métodos de identificação utilizados. Ficou também claramente demonstrado que o gene iaaL associado à patogenicidade não é exclusivo de P. savastanoi, levantando a hipótese de esta espécie não ser a única responsável pelo aparecimento de nódulos em oliveira. /ABSTRACT ‐ Pseudomonas savastanoi cause a disease known a olive knot. ln this study a polyphasic approach was applied for the characterization of strains (IAA producers) isolated from three varieties of olive trees. Both classic phenotypic and molecular tests were applied, in particular for detection of the iaaL gene, PCR‐RFLPs of 16S rDNA and genomic fingerprinting using BOX‐, ERIC‐ and REP‐PCR. High fenetic heterogeneity was obtained among isolated strains identified as P. savastanoi. Sequencing of 168 rDNA revealed that only one isolated strain showed homology with P. savastanoi, while the remaining strains were homologous to Pantoea agglomerans, Pantoea oleae and Erwinia toletana, proving the lack of fidelity of the other identification methods used. lt was also clearly demonstrated that iaaL gene associated with the disease is not exclusive of P. savastanoi, which suggests that this species is not the only one responsible for the “olive knot".
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Goulart, Marcela Cristina 1988. "Desenvolvimento de metodologia de detecção e identificação de fitobactérias em sementes de soja [Glycine max (L.) Merril] por primers espécie-específicos." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317295.

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Orientador: Suzete Aparecida Lanza Destéfano
Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-24T19:29:47Z (GMT). No. of bitstreams: 1 Goulart_MarcelaCristina_M.pdf: 1843141 bytes, checksum: 317126844277b642b5edc69d405b000b (MD5) Previous issue date: 2014
Resumo: A soja é considerada uma das culturas mais importantes no Brasil, em função de seu alto valor sócio-econômico, determinado pelas inúmeras aplicações de seus produtos e subprodutos e consequente expressão no mercado interno e externo. No entanto, a cultura desta oleaginosa é frequentemente ameaçada com a ocorrência de um vasto número de doenças, que podem acarretar depreciação do produto, redução no rendimento e perdas econômicas para os produtores. Dentre as principais doenças bacterianas que afetam a cultura da soja, destacam-se a pústula bacteriana, causada por Xanthomonas axonopodis pv. glycines (Xag); o crestamento bacteriano, causado por Pseudomonas savastanoi pv. glycines (Psg); e a murcha de Curtobacterium causada por Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), ocasionando perdas na produção de até 40%. A condição sanitária das sementes é extremamente importante se considerarmos que elas são veículos desses agentes fitopatogênicos que nelas podem se alojar e serem levados ao campo, provocando redução na germinação e vigor, e originando focos primários de infecção de doenças. O presente trabalho teve por objetivo desenvolver nova metodologia de diagnóstico com o uso das técnicas moleculares que permitissem detectar e identificar a presença de Psg, Xag e Cff em sementes de soja por meio do desenvolvimento de primers espécie específicos. Os primers desenhados a partir de sequências da região espaçadora 16S-23S RNAr, mostraram-se altamente específicos e sensíveis. O par de primers Curto2f/p322anti gerou um fragmento de 675 pb e capacidade de detecção a partir de 0,01 ng de DNA genômico e aproximadamente 5x103 UFC/PCR; o par de primers Psgl/p322anti gerou um fragmento de 500 pb e o grau mínimo de sensibilidade foi de 1 pg de DNA genômico e cerca de 80 UFC/PCR; o par de primers Xanth2f/p322anti gerou um fragmento de 545 pb e capacidade de detecção a partir de 1 ng de DNA genômico e cerca de 700 UFC/PCR. Posteriormente, as sementes de soja foram infectadas artificialmente nas condições de 1; 0,5 e 0,1% de infecção. Nas amplificações com os primers espécie-específicos desenvolvidos, foi possível detectar as fitobactérias em todos os níveis de infecção testados diretamente do extrato bruto, e nas amplificações após o enriquecimento do extrato (BIO-PCR) o sinal positivo foi potencializado
Abstract: Soybean is considered one of the most important crops in Brazil, due to its high socio-economic value, determined by its several products and sub products and its significant expression on the internal and external market. However, this oleaginous plant is often affected by the occurrence of different diseases, which cause depreciation of the product, reduction in yield and substantial economic losses. Among the main bacterial diseases, the bacterial pustule, caused by Xanthomonas axonopodis pv. glycines (Xag), the bacterial blight caused by Pseudomonas savastanoi pv. glycines (Psg), and bacterial tan spot caused by Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), producing yield losses of up to 40%. The seed health is extremely important since they are considered vehicles of pathogenic agents which can be led to the field, causing germination reduction and vigor; and yielding primary infection of the diseases. This study aimed to develop a new method of diagnosis using molecular tools to detect and identify Psg, Xag or Cff in soybean seeds, through species-specific primers. The primers were designed from sequences of the 16S-23S rRNA and they were highly specific and sensitive. The pair of primers Curto2f/p322anti generated a fragment of 675 bp and was able of detecting down to 0.01 ng of genomic DNA and about 5x103 CFU/PCR; the primer set Psgl/p322anti produced a fragment of 500 bp and reached a detection limit of 1 pg of genomic DNA and about 80 CFU/PCR; and Xanth2f/p322anti yielded a fragment of 545 bp and could detect up to 1 ng of genomic DNA and about 700 CFU/PCR. Subsequently, soybean seeds were artificially infected in the following conditions: 1, 0,5% and 0,1% infection. In the amplifications using the species-specific primers, it was possible to detect the three different phytobacteria at all tested levels of infection directly of the crude extract and in the amplifications after enrichment of the extract (BIO-PCR), the positive signal was enhanced
Mestrado
Genetica de Microorganismos
Mestra em Genética e Biologia Molecular
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CERBONESCHI, MATTEO. "Chiavi molecolari per la caratterizzazione e la comprensione delle interazioni ospite patogeno in Pseudomonas savastanoi." Doctoral thesis, 2009. http://hdl.handle.net/2158/780682.

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SANTILLI, ELENA. "Sviluppo di metodi molecolari per la determinazione e l’identificazione di batteri fitopatogeni." Doctoral thesis, 2006. http://hdl.handle.net/2158/590097.

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L’agente causale della “Rogna” dell’olivo, oggi classificato come Pseudomonas savastanoi pv. savastanoi, è un batterio fitopatogeno da lungo tempo oggetto di studi e ricerche a livello mondiale. Paradossalmente ancora poco è noto sui determinanti biochimici e molecolari della sua patogenicità e virulenza. A tutt’oggi infatti non sono state del tutto decodificate le basi molecolari della specificità d’ospite della specie P. savastanoi, che permettono alle tre pathovar savastanoi, nerii e fraxini di attaccare rispettivamente l’Olivo, l’Oleandro e il Frassino. Il lavoro svolto nella presente tesi di Dottorato è partito dalla convinzione che attraverso l’approccio biotecnologico è possibile, ed in parte lo è già stato, conoscere le basi molecolari del dialogo biochimico tra il fitopatogeno e la pianta, ottenendo indicazioni essenziali su “come” e “dove” interrompere il ciclo di malattia, attraverso lo sviluppo e l’applicazione di strategie di lotta, possibilmente di tipo preventivo o che comunque consentano una drastica riduzione nell’uso dei pesticidi chimici convenzionali. Tra le strategie preventive che garantiscono risultati eccellenti nella gestione delle malattie in generale, e quindi anche nel campo della Patologia vegetale, è sicuramente da citare la corretta e tempestiva identificazione/diagnosi della presenza dell’agente infettivo causa di malattia, abnche e soprattutto in una fase precoce quando i sintomi non siano ancora presenti. Ciò riteniamo sia particolarmente importante nel caso della Rogna dell'Olivo, poiché il controllo di questa malattia presenta molte difficoltà e la lotta finora si e' basata per lo più su criteri preventivi, agronomico-colturali, oltre ai consueti trattamenti con sali di rame. Peraltro, esistono dati fondati sulla biologia di questo batterio e che hanno dimostrato la sua presenza anche su materiale vegetale asintomatico, poiché è stato accertato che P. savastanoi può sopravvivere come epifita sulla pianta e così essere trasportato anche a lunghe distanze attraverso il movimento del materiale vegetale destinato alla propagazione vegetativa della pianta. Pertanto, con i risultati ottenuti in questo lavoro di tesi si è voluto portare un contributo nel campo della diagnostica molecolare, sviluppando dei metodi molecolari per la determinazione e l'identificazione di alcune delle pathovar della specie P. savastanoi, applicabile anche direttamente su matrice vegetale. La strategia adottata per il raggiungimento di tale obiettivo è stata quella di cercare di individuare “impronte genomiche”, peculiari di ciascuna delle pathovar di P. savastanoi considerate e che fossero o che potessero essere implicate nella loro patogenicità e virulenza, nonché nella determinazione della cerchia d’ospite, a garanzia del fatto che l’identificazione del batterio fosse indissolubilmente legata alla sua capacità di causare malattia sull’ospite.
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Mina, João Diogo Calado Martins. "Endo- and epiphytic bacteria from olive tree phyllosphere with biocontrol abilities against olive knot." Doctoral thesis, 2020. http://hdl.handle.net/1822/76667.

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Tese de Doutoramento em Ciências (Especialidade em Biologia)
A tuberculose da oliveira, causada pela bactéria Pseudomonas savastanoi pv. savastanoi (Pss), é uma importante doença do olival ainda sem tratamento conhecido. Esta doença afeta a parte aérea das oliveiras e caracteriza-se por um crescimento anormal dos tecidos, principalmente no tronco e ramos. Neste trabalho foi caracterizada a comunidade bacteriana que habita a filosfera da oliveira, de modo a elucidar o seu possível papel na defesa da planta contra a tuberculose da oliveira. Uma abordagem dependente de cultivo foi usada para descrever as populações da superfície (epífitos) e do interior (endófitos) de folhas, caules e nódulos de duas cultivares com diferentes suscetibilidades a esta doença. Para alguns dos isolados obtidos foi testada a sua capacidade antagonista contra Pss em ensaios in vitro, tendo os mecanismos associados a este antagonismo sido também avaliados. A eficácia do isolado mais promissor, Bacillus amyloliquefaciens P41, na redução do desenvolvimento da doença e na melhoria do fitness da planta foi avaliada através de ensaios in planta. No geral, a comunidade bacteriana da filosfera da oliveira inclui membros pertencentes principalmente a Proteobacteria, em particular a Gammaproteobacteria. A composição bacteriana foi principalmente afetada pela cultivar do hospedeiro e em menor grau pelo órgão, que teve um maior impacto nos epífitos. Adicionalmente, cada cultivar/órgão foi aparentemente seletiva através de OTUs bacterianos específicos. A tuberculose da oliveira revelou ter também um impacto na estrutura da comunidade bacteriana, mas com diferentes efeitos, dependentes da cultivar e do habitat na planta hospedeira. Na verdade, o seu efeito foi mais notório na cultivar mais suscetível à doença e nos endófitos. Um total de 27 isolados inibiram significativamente o crescimento de Pss, tendo os isolados com uma maior capacidade antagonista sido isolados da cultivar suscetível. Esta capacidade antagonista deveu-se provavelmente à produção de compostos voláteis, enzimas líticas e sideróforos. B. amyloliquefaciens P41 reduziu a severidade da doença em até 43.7% e a população de Pss em até 26.8%, e simultaneamente melhorou o fitness da planta hospedeira, podendo ser possivelmente considerado um candidato promissor no controlo da tuberculose da oliveira. Estudos adicionais são necessários para identificar o papel funcional destas bactérias e dos mecanismos envolvidos na proteção da planta hospedeira contra a tuberculose da oliveira.
Olive knot (OK), caused by Pseudomonas savastanoi pv. savastanoi (Pss), is an important olive orchard disease with still no treatment known. This disease affects the aerial part of the olive trees and is characterized by overgrowth formations (knots) mainly on trunk and branches. In this work was characterized the bacterial community inhabiting the olive tree phyllosphere, in order to elucidate its possible role on plant defense against OK disease. A culture-dependent approach was used to describe the bacterial populations in (epiphytes) and on (endophytes) leaves, twigs and knots of two cultivars with different susceptibility to OK disease. Some of the isolates obtained were screened for their antagonistic effect against Pss in in vitro assays, and their mechanisms were also evaluated. The efficacy of the most promising isolate, Bacillus amyloliquefaciens P41, in reducing OK development and improving plant fitness was evaluated through in planta assays. Overall, the bacterial community of olive tree phyllosphere comprised members belonging mainly to Proteobacteria, in particular Gammaproteobacteria. Bacterial composition was primarily impact by host cultivar, and, to a lesser extent, by plant organ which had a more control over epiphytes. In addition, each cultivar/organ apparently was selective towards specific bacterial OTUs. OK disease showed also to have an impact on the structure of bacterial communities, but with variable effects depending on the host cultivar and plant habitat. Indeed, its effect was most notorious in OK-susceptible cultivar and within endophytes. A total of 27 isolates inhibited significantly Pss growth, being the ones with the greatest antagonistic activity from the tissues surface of OK-susceptible cultivar. Such ability was potentially due to the production of volatile compounds, lytic enzymes and siderophores. B. amyloliquefacients P41 reduced OK disease’s severity up to 43.7% and Pss population size up to 26.8% and simultaneously increased plant fitness, suggesting to be a promising candidate for controlling OK disease. More research are still required to identify the functional role of these bacteria and the mechanisms involved in conferring host plant protection to OK disease.
This research was partially supported by FEDER funds through COMPETE (Programa Operacional Factores de Competitividade), national funds through FCT (Fundacao para a Ciencia e a Tecnologia) and by Horizon 2020, the European Union's Framework Programme for Research and Innovation, within the project PRIMA/0002/2018 INTOMED - Innovative tools to combat crop pests in the Mediterranean, and PTDC/A5P-PLA/31133/2017 MicOlives - Exploiting plant induced resistance by beneficial fungi as a new sustainable approach to olive crop protection. D. Mina thanks FCT, POPH-QREN and FSE for SFRH-BD-105341/2014 grant and also the COST Action FA1405 for two short-term scientific mission (51-5M) grant.
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Book chapters on the topic "Pseudomonas savastanoi"

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Hassani, D., R. Buonaurio, and A. Tombesi. "Response of Some Olive Cultivars, Hybrid and Open Pollinated Seedlings to Pseudomonas savastanoi pv. savastanoi." In Pseudomonas syringae and related pathogens, 489–94. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0133-4_54.

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López, M. M., E. Bertolini, P. Caruso, R. Penyalver, A. Olmos, J. M. Quesada, and M. Cambra. "Optimising PCR Detection of Ralstonia solanacearum and Pseudomonas savastanoi pv. savastanoi: Two Models, Two Approaches." In Pseudomonas syringae and related pathogens, 523–29. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0133-4_58.

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Kosuge, Tsune, Curtis J. Palm, Steven V. Hutcheson, N. Louise, E. Glass, and Tetsuji Yamada. "pIAA, A Virulence Plasmid in Pseudomonas Savastanoi." In Plasmids in Bacteria, 807–13. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_56.

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Bella, P., V. Catara, L. Sutra, G. Guarino, G. Cirvilleri, and L. Gardan. "Phenotypic Characteristics of Pseudomonas savastanoi Strains from Various Hosts." In Pseudomonas syringae and related pathogens, 681–86. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0133-4_75.

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Moretti, C., P. Ferrante, T. Hosni, F. Valentini, A. D'Onghia, M'Barek Fatmi, and R. Buonaurio. "Characterization of Pseudomonas savastanoi pv. Savastanoi Strains Collected from Olive Trees in Different Countries." In Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics, 321–29. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6901-7_33.

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Quesada, J. M., R. Penyalver, and M. M. López. "Epidemiological Basis for an Efficient Control of Pseudomonas savastanoi pv. savastanoi on Olive Trees." In Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics, 57–64. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6901-7_5.

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Penyalver, R., E. Bertolini, A. Olmos, A. García, M. Cambra, and M. M. López. "Detection of Pseudomonas savastanoi pv. savastanoi (Pss) on Asymptomatic Olive Plant Tissues by Enrichment-PCR." In Plant Pathogenic Bacteria, 421–24. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0003-1_94.

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Sisto, A., M. G. Cipriani, and M. Morea. "Sequence Analysis of the hrpC Operon and the hrpE Gene of Pseudomonas syringae subsp. savastanoi." In Pseudomonas syringae and related pathogens, 405–10. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0133-4_44.

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Ott, P. G., Z. Klement, I. Nagy, and A. L. Ádám. "Lanthanum Inhibits Programmed Cell Death but not Resistance in the Tobacco — Pseudomonas savastanoi pv. phaseolicola Incompatible Interaction." In Pseudomonas syringae and related pathogens, 335–44. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0133-4_36.

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Sisto, A., M. G. Cipriani, M. Morea, S. L. Lonigro, and P. Lavermicocca. "Antagonistic Activity of Pseudomonas syringae subsp. savastanoi: Preliminary Results on the Identification of a Plasmid-located Genetic Determinant." In Pseudomonas syringae and related pathogens, 117–24. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0133-4_13.

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Conference papers on the topic "Pseudomonas savastanoi"

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Tarakanov., R. I., A. N. Ignatov, and F. S. Dzhalilov. "Development of bacteriophage agent for soyean bacterial blight control." In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-136.

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Abstract:
There is a description for the method for development of bacteriophage agent to control bacterial blight of soybean caused by Pseudomonas savastanoi pv. glycinea. Biological effectiveness of bacteriophage application was about 75% that was approximately the same as for test chemical bactericides.
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