Journal articles: 'Pseudoalteromonas'

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Wang, Xiyan, Thomas Isbrandt, Mikael Lenz Strube, Sara Skøtt Paulsen, Maike Wennekers Nielsen, Yannick Buijs, Erwin M. Schoof, Thomas Ostenfeld Larsen, Lone Gram, and Sheng-Da Zhang. "Chitin Degradation Machinery and Secondary Metabolite Profiles in the Marine Bacterium Pseudoalteromonas rubra S4059." Marine Drugs 19, no. 2 (February 12, 2021): 108. http://dx.doi.org/10.3390/md19020108.

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Genome mining of pigmented Pseudoalteromonas has revealed a large potential for the production of bioactive compounds and hydrolytic enzymes. The purpose of the present study was to explore this bioactivity potential in a potent antibiotic and enzyme producer, Pseudoalteromonas rubra strain S4059. Proteomic analyses (data are available via ProteomeXchange with identifier PXD023249) indicated that a highly efficient chitin degradation machinery was present in the red-pigmented P. rubra S4059 when grown on chitin. Four GH18 chitinases and two GH20 hexosaminidases were significantly upregulated under these conditions. GH19 chitinases, which are not common in bacteria, are consistently found in pigmented Pseudoalteromonas, and in S4059, GH19 was only detected when the bacterium was grown on chitin. To explore the possible role of GH19 in pigmented Pseudoalteromonas, we developed a protocol for genetic manipulation of S4059 and deleted the GH19 chitinase, and compared phenotypes of the mutant and wild type. However, none of the chitin degrading ability, secondary metabolite profile, or biofilm-forming capacity was affected by GH19 deletion. In conclusion, we developed a genetic manipulation protocol that can be used to unravel the bioactive potential of pigmented pseudoalteromonads. An efficient chitinolytic enzyme cocktail was identified in S4059, suggesting that this strain could be a candidate with industrial potential.

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Lim, Su-Jin, Dae-jin Min, Sohee Kim, Jihye Lee, Eun-Soo Lee, Hyuk Kim, Sung-Yoen Cho, et al. "Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis." Marine Drugs 19, no. 11 (October 28, 2021): 612. http://dx.doi.org/10.3390/md19110612.

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An ubiquinone derivative, pseudoalteromone A (1), has been isolated from two marine-derived Pseudoalteromonas spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrated by assessing the intracellular and extracellular melanin content and cellular tyrosinase activity in the B16 cell line, Melan-a mouse melanocyte cell line, and MNT-1 human malignant melanoma cell line. Treatment with pseudoalteromone A (40 μg/mL) for 72 h reduced α-melanocyte-stimulating hormone (α-MSH)-induced intracellular melanin production by up to 44.68% in B16 cells and 38.24% in MNT-1 cells. Notably, pseudoalteromone A induced a concentration-dependent reduction in cellular tyrosinase activity in B16 cell, and Western blot analyses showed that this inhibitory activity was associated with a significant decrease in protein levels of tyrosinase and tyrosinase-related protein 1 (Tyrp-1), suggesting that pseudoalteromone A exerts its anti-melanogenesis activity through effects on melanogenic genes. We further evaluated the skin-whitening effect of pseudoalteromone A in the three-dimensional (3D) pigmented-epidermis model, MelanoDerm, and visualized the 3D distribution of melanin by two-photon excited fluorescence imaging in this human skin equivalent. Collectively, our findings suggest that pseudoalteromone A inhibits tyrosinase activity and expression and that this accounts for its anti-melanogenic effects in melanocytes.

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Nam, Young-Do, Ho-Won Chang, Ja Ryeong Park, Hyuk-Yong Kwon, Zhe-Xue Quan, Yong-Ha Park, Jung-Sook Lee, Jung-Hoon Yoon, and Jin-Woo Bae. "Pseudoalteromonas marina sp. nov., a marine bacterium isolated from tidal flats of the Yellow Sea, and reclassification of Pseudoalteromonas sagamiensis as Algicola sagamiensis comb. nov." International Journal of Systematic and Evolutionary Microbiology 57, no. 1 (January 1, 2007): 12–18. http://dx.doi.org/10.1099/ijs.0.64523-0.

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Two Gram-negative, motile and strictly aerobic marine bacteria were isolated from a tidal flat sediment sample obtained from Dae-Chun, Chung-Nam, Korea. They were preliminarily identified as Pseudoalteromonas-like bacteria, based on 16S rRNA gene sequence analysis showing nearly identical sequences (>99.7 % sequence similarity) and the highest similarity (98.4 %) to the species Pseudoalteromonas undina. Some phenotypic features of the newly isolated strains were similar to those of members of the genus Pseudoalteromonas, but several physiological and chemo-taxonomical properties readily distinguished the new isolates from previously described species. DNA–DNA hybridization with type strains of phylogenetically closely related species demonstrated that the isolates represent a novel Pseudoalteromonas species, for which the name Pseudoalteromonas marina sp. nov. is proposed, with the type strain mano4T (=KCTC 12242T=DSM 17587T). In addition, on the basis of this study and polyphasic data obtained from previous work, it is proposed that the species Pseudoalteromonas sagamiensis should be reclassified as Algicola sagamiensis comb. nov. and that strain B-10-31T (=DSM 14643T=JCM 11461T) be designated the type strain.

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Park, Yoon-Dong, Keun Sik Baik, Hana Yi, Kyung Sook Bae, and Jongsik Chun. "Pseudoalteromonas byunsanensis sp. nov., isolated from tidal flat sediment in Korea." International Journal of Systematic and Evolutionary Microbiology 55, no. 6 (November 1, 2005): 2519–23. http://dx.doi.org/10.1099/ijs.0.63750-0.

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A Gram-negative, motile, strictly aerobic, violet-pigment-producing bacterium, designated strain FR1199T, was isolated from tidal flat sediment of Byunsan, South Korea. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain FR1199T represents a distinct line of descent within the genus Pseudoalteromonas. The phenotypic features of strain FR1199T were similar to those of Pseudoalteromonas phenolica and Pseudoalteromonas luteoviolacea, but several physiological and chemotaxonomical properties readily distinguished strain FR1199T from these species. Major fatty acids were straight-chain saturated (C16 : 0) and monounsaturated C18 : 1 ω7c fatty acids. The DNA G+C content was 39 mol%. On the basis of polyphasic evidence, it is concluded that the isolate represents a novel species within the genus Pseudoalteromonas, for which the name Pseudoalteromonas byunsanensis sp. nov. is proposed. The type strain is FR1199T (=JCM 12483T=KCTC 12274T).

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Oh, Yong-Sik, A.-Rum Park, Je-Kwan Lee, Chae-Sung Lim, Jae-Soo Yoo, and Dong-Hyun Roh. "Pseudoalteromonas donghaensis sp. nov., isolated from seawater." International Journal of Systematic and Evolutionary Microbiology 61, no. 2 (February 1, 2011): 351–55. http://dx.doi.org/10.1099/ijs.0.022541-0.

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A Gram-negative, rod-shaped, motile and aerobic bacterium, designated strain HJ51T, was isolated from a seawater sample from the East Sea, near South Korea. The isolate grew slowly at 4 °C, was able to grow at 40 °C, required NaCl and grew optimally at pH 6.5–7.0. The G+C content of the genomic DNA was 41.8 mol%. The major fatty acids were summed feature 4 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and summed feature 7 (C18 : 1 ω7c, C18 : 1 ω9t and/or C18 : 1 ω12t). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HJ51T belonged to the genus Pseudoalteromonas and had 91.7–98.9 % 16S rRNA gene sequence similarity with type strains of species of the genus Pseudoalteromonas. Strain HJ51T had 7.2 % DNA–DNA relatedness with Pseudoalteromonas mariniglutinosa DSM 15203T and 12.9 % with Pseudoalteromonas prydzensis DSM 14232T. On the basis of the phenotypic, phylogenetic and genomic data, strain HJ51T represents a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas donghaensis sp. nov. is proposed. The type strain is HJ51T (=KCTC 22219T=LMG 24469T).

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Zhao, Chang-Hui, Jing-Jing Luo, Ting Gong, Xiang-Ling Huang, De-Zan Ye, and Zhu-Hua Luo. "Pseudoalteromonas xiamenensis sp. nov., a marine bacterium isolated from coastal surface seawater." International Journal of Systematic and Evolutionary Microbiology 64, Pt_2 (February 1, 2014): 444–48. http://dx.doi.org/10.1099/ijs.0.050229-0.

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A Gram-negative, oxidase- and catalase-positive, rod-shaped, non-spore-forming, motile, aerobic bacterium, designated Y2T, was isolated from surface seawater of Yundang Lake, Xiamen, China. The strain was able to grow in the presence of 0.5–6.0 % NaCl (optimum 1.0–1.5 %), at pH 5–10 (optimum pH 8) and at 10–40 °C (optimum 25 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Y2T belongs to the genus Pseudoalteromonas , with the highest sequence similarity of 94.9 % to Pseudoalteromonas tunicata D2T; within the genus Pseudoalteromonas , it showed the lowest similarity of 92.8 % to Pseudoalteromonas denitrificans ATCC 43337T. The G+C content of the chromosomal DNA of strain Y2T was 45.1 mol%. The predominant fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, C12 : 0 3-OH and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The only respiratory quinone detected was Q-8. Based on the phylogenetic and phenotypic characteristics, strain Y2T represents a novel species of the genus Pseudoalteromonas , for which the name Pseudoalteromonas xiamenensis sp. nov. is proposed; the type strain is Y2T ( = CGMCC 1.12157T = JCM 18779T).

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Yu, Zhiliang, Yajuan Ding, Jianhua Yin, Dongliang Yu, Jiadi Zhang, Mengting Zhang, Mengdan Ding, Weihong Zhong, Juanping Qiu, and Jun Li. "Dissemination of Genetic Acquisition/Loss Provides a Variety of Quorum Sensing Regulatory Properties in Pseudoalteromonas." International Journal of Molecular Sciences 19, no. 11 (November 18, 2018): 3636. http://dx.doi.org/10.3390/ijms19113636.

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A bstract: Quorum sensing (QS) enables single-celled bacteria to communicate with chemical signals in order to synchronize group-level bacterial behavior. Pseudoalteromonas are marine bacteria found in versatile environments, of which QS regulation for their habitat adaptation is extremely fragmentary. To distinguish genes required for QS regulation in Pseudoalteromonas, comparative genomics was deployed to define the pan-genomics for twelve isolates and previously-sequenced genomes, of which acyl-homoserine lactone (AHL)-based QS traits were characterized. Additionally, transposon mutagenesis was used to identify the essential QS regulatory genes in the selected Pseudoalteromonas isolate. A remarkable feature showed that AHL-based colorization intensity of biosensors induced by Pseudoalteromonas most likely correlates with QS regulators genetic heterogeneity within the genus. This is supported by the relative expression levels of two of the main QS regulatory genes (luxO and rpoN) analyzed in representative Pseudoalteromonas isolates. Notably, comprehensive QS regulatory schema and the working model proposed in Pseudoalteromonas seem to phylogenetically include the network architectures derived from Escherichia coli, Pseudomonas, and Vibrio. Several associated genes were mapped by transposon mutagenesis. Among them, a right origin-binding protein-encoding gene (robp) was functionally identified as a positive QS regulatory gene. This gene lies on a genomic instable region and exists in the aforementioned bioinformatically recruited QS regulatory schema. The obtained data emphasize that the distinctly- and hierarchically-organized mechanisms probably target QS association in Pseudoalteromonas dynamic genomes, thus leading to bacterial ability to accommodate their adaption fitness and survival advantages.

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Kim, Dockyu, Ha Ju Park, and Hyun Park. "Construction of Pseudoalteromonas - Escherichia coli shuttle vector based on a small plasmid from the marine organism Pseudoalteromonas." Korean Journal of Microbiology 52, no. 1 (March 31, 2016): 110–15. http://dx.doi.org/10.7845/kjm.2016.5054.

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9

Navarro-Torre, Salvadora, Lorena Carro, Ignacio D. Rodríguez-Llorente, Eloísa Pajuelo, Miguel Ángel Caviedes, José Mariano Igual, Hans-Peter Klenk, and Maria del Carmen Montero-Calasanz. "Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth-promoting bacterium with potential use in phytoremediation." International Journal of Systematic and Evolutionary Microbiology 70, no. 5 (May 1, 2020): 3287–94. http://dx.doi.org/10.1099/ijsem.0.004167.

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Strain RA15T was isolated from the rhizosphere of the halophyte plant Arthrocnemum macrostachyum growing in the Odiel marshes (Huelva, Spain). RA15T cells were Gram stain-negative, non-spore-forming, aerobic rods and formed cream-coloured, opaque, mucoid, viscous, convex, irregular colonies with an undulate margin. Optimal growth conditions were observed on tryptic soy agar (TSA) plates supplemented with 2.5 % NaCl (w/v) at pH 7.0 and 28 °C, although it was able to grow at 4–32 °C and at pH values of 5.0–9.0. The NaCl tolerance range was from 0 to 15 %. The major respiratory quinone was Q8 but Q9 was also present. The most abundant fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C17 : 1 ω8c and C16 : 0. The polar lipids profile comprised phosphatidylglycerol and phosphatidylethanolamine as the most abundant representatives. Phylogenetic analyses confirmed the well-supported affiliation of strain RA15T within the genus Pseudoalteromonas , close to the type strains of Pseudoalteromonas neustonica , Pseudoalteromonas prydzensis and Pseudoalteromonas mariniglutinosa . Results of comparative phylogenetic and phenotypic studies between strain RA15T and its closest related species suggest that RA15T could be a new representative of the genus Pseudoalteromonas , for which the name Pseudoalteromonas rhizosphaerae sp. nov. is proposed. The type strain is RA15T (=CECT 9079T=LMG 29860T). The whole genome has 5.3 Mb and the G+C content is 40.4 mol%.

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Doghri, Ibtissem, Emilie Portier, Florie Desriac, Jean Michel Zhao, Alexis Bazire, Alain Dufour, Vincent Rochette, Sophie Sablé, and Isabelle Lanneluc. "Anti-Biofilm Activity of a Low Weight Proteinaceous Molecule from the Marine Bacterium Pseudoalteromonas sp. IIIA004 against Marine Bacteria and Human Pathogen Biofilms." Microorganisms 8, no. 9 (August 25, 2020): 1295. http://dx.doi.org/10.3390/microorganisms8091295.

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Pseudoalteromonas bacteria are known as potential bioactive metabolite producers. Because of the need to obtain natural molecules inhibiting the bacterial biofilms, we investigated the biofilm inhibitory activity of the marine bacterium Pseudoalteromonas sp. IIIA004 against the pioneer surface colonizer Roseovarius sp. VA014. The anti-biofilm activity from the culture supernatant of Pseudoalteromonas sp. IIIA004 (SNIIIA004) was characterized in microtiter plates (static conditions/polystyrene surface) and in flow cell chambers (dynamic conditions/glass surface). The Pseudoalteromonas exoproducts exhibited an inhibition of Roseovarius sp. VA014 biofilm formation as well as a strong biofilm dispersion, without affecting the bacterial growth. Microbial adhesion to solvent assays showed that SNIIIA004 did not change the broad hydrophilic and acid character of the Roseovarius strain surface. Bioassay-guided purification using solid-phase extraction and C18 reverse-phase-high-performance liquid chromatography (RP-HPLC) was performed from SNIIIA004 to isolate the proteinaceous active compound against the biofilm formation. This new anti-biofilm low weight molecule (< 3kDa), named P004, presented a wide spectrum of action on various bacterial biofilms, with 71% of sensitive strains including marine bacteria and human pathogens. Pseudoalteromonas sp. IIIA004 is a promising source of natural anti-biofilm compounds that combine several activities.

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Matsuyama, Hidetoshi, Kazuya Sawazaki, Hideki Minami, Hirokazu Kasahara, Keiji Horikawa, and Isao Yumoto. "Pseudoalteromonas shioyasakiensis sp. nov., a marine polysaccharide-producing bacterium." International Journal of Systematic and Evolutionary Microbiology 64, Pt_1 (January 1, 2014): 101–6. http://dx.doi.org/10.1099/ijs.0.055558-0.

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A novel exopolysaccharide-producing bacterium, designated strain SE3T, was isolated from Pacific Ocean sediment. The strain was Gram-stain-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na+ for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The DNA G+C content was 46.9 mol%. 16S rRNA gene sequence analysis suggested that strain SE3T is a member of the genus Pseudoalteromonas . Strain SE3T exhibited close phylogenetic affinity to Pseudoalteromonas arabiensis JCM 17292T (99.0 % 16S rRNA gene sequence similarity), Pseudoalteromonas lipolytica LMEB 39T (98.39 %) and Pseudoalteromonas donghaensis HJ51T (97.65 %). The DNA–DNA reassociation values between strain SE3T and P. arabiensis JCM 17292T, P. lipolytica JCM 15903T and P. donghaensis LMG 24469T were 31, 26 and 44 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA relatedness data, the new isolate merits classification as a representative of novel species, for which the name Pseudoalteromonas shioyasakiensis is proposed. The type strain is SE3T ( = JCM 18891T = NCIMB 14852T).

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Chen, Yu-Hsin, Jimmy Kuo, Jui-Hsin Su, Tsong-Long Hwang, Yung-Husan Chen, Chia-Hung Lee, Ching-Feng Weng, and Ping-Jyun Sung. "Pseudoalteromone B: A Novel 15C Compound from a Marine Bacterium Pseudoalteromonas sp. CGH2XX." Marine Drugs 10, no. 12 (July 20, 2012): 1566–71. http://dx.doi.org/10.3390/md10071566.

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Xu, Xue-Wei, Yue-Hong Wu, Chun-Sheng Wang, Xiao-Hui Gao, Xiao-Gu Wang, and Min Wu. "Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary." International Journal of Systematic and Evolutionary Microbiology 60, no. 9 (September 1, 2010): 2176–81. http://dx.doi.org/10.1099/ijs.0.017673-0.

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A strictly aerobic, Gram-negative, non-pigmented bacterial strain, designated LMEB 39T, was isolated from a seawater sample collected from the Yangtze River estuary near the East China Sea and was examined physiologically, chemotaxonomically and phylogenetically. The novel isolate was motile by a single polar flagellum and positive for nitrate reduction and decomposition of casein, gelatin, Tween 20 and Tween 80, but negative for indole production. Chemotaxonomic analysis revealed ubiquinone-8 as the predominant respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. The major fatty acids were C16 : 1 ω7c/iso-C15 : 0 2-OH, C16 : 0, C18 : 1 ω7c, C12 : 0 3-OH, C17 : 1 ω8c and C17 : 0. The genomic DNA G+C content was 42.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Pseudoalteromonas. Strain LMEB 39T exhibited the closest phylogenetic affinity to Pseudoalteromonas byunsanensis JCM 12483T (97.4 % sequence similarity). The DNA–DNA reassociation values between strain LMEB 39T and P. byunsanensis JCM 12483T and Pseudoalteromonas undina DSM 6065T (97.2 % sequence similarity) were 31.7 and 30.3 %, respectively. On the basis of phenotypic and genotypic data, strain LMEB 39T represents a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas lipolytica sp. nov. is proposed; the type strain is LMEB 39T (=CGMCC 1.8499T=JCM 15903T).

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Ivanova, Elena P., Ludmila S. Shevchenko, Tomoo Sawabe, Anatolii M. Lysenko, Vasilii I. Svetashev, Nataliya M. Gorshkova, Masataka Satomi, Richard Christen, and Valery V. Mikhailov. "Pseudoalteromonas maricaloris sp. nov., isolated from an Australian sponge, and reclassification of [Pseudoalteromonas aurantia] NCIMB 2033 as Pseudoalteromonas flavipulchra sp. nov." International Journal of Systematic and Evolutionary Microbiology 52, no. 1 (January 1, 2002): 263–71. http://dx.doi.org/10.1099/00207713-52-1-263.

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Skovhus, Torben L., Niels B. Ramsing, Carola Holmström, Staffan Kjelleberg, and Ingela Dahllöf. "Real-Time Quantitative PCR for Assessment of Abundance of Pseudoalteromonas Species in Marine Samples." Applied and Environmental Microbiology 70, no. 4 (April 2004): 2373–82. http://dx.doi.org/10.1128/aem.70.4.2373-2382.2004.

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ABSTRACT A real-time quantitative PCR (RTQ-PCR) method for measuring the abundance of Pseudoalteromonas species in marine samples is presented. PCR primers targeting a Pseudoalteromonas-specific region of the 16S rRNA gene were tested at three different levels using database searches (in silico), a selection of pure cultures (in vitro), and a combined denaturing gradient gel electrophoresis and cloning approach on environmental DNA (in situ). The RTQ-PCR method allowed for the detection of SYBR Green fluorescence from double-stranded DNA over a linear range spanning six orders of magnitude. The detection limit was determined as 1.4 fg of target DNA (1,000 gene copies) measured in the presence of 20 ng of nontarget DNA from salmon testes. In this study, we discuss the importance of robust post-PCR analyses to overcome pitfalls in RTQ-PCR when samples from different complex marine habitats are analyzed and compared on a nonroutine basis. Representatives of the genus Pseudoalteromonas were detected in samples from all investigated habitats, suggesting a widespread distribution of this genus across many marine habitats (e.g., seawater, rocks, macroalgae, and marine animals). Three sample types were analyzed by RTQ-PCR to determine the relative abundance of Pseudoalteromonas ribosomal DNA (rDNA) compared to the total abundance of eubacterial rDNA. The rDNA fractions of Pseudoalteromonas compared to all Eubacteria were 1.55% on the green alga Ulva lactuca, 0.10% on the tunicate Ciona intestinalis, and 0.06% on the green alga Ulvaria fusca.

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Chen, Yu-Hsin, Mei-Chin Lu, Yu-Chia Chang, Tsong-Long Hwang, Wei-Hsien Wang, Ching-Feng Weng, Jimmy Kuo, and Ping-Jyun Sung. "Pseudoalteromone A: a novel bioactive ubiquinone from a marine bacterium Pseudoalteromonas sp. CGH2XX (Pseudoalteromonadaceae)." Tetrahedron Letters 53, no. 13 (March 2012): 1675–77. http://dx.doi.org/10.1016/j.tetlet.2012.01.104.

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Setiyono, Edi, Marcelinus Alfasisurya Setya Adhiwibawa, Matheus Randy Prabowo, and Tatas H. P. Brotosudarmo. "Characterization of Tambjamines Pigment from Marine Bacterium Pseudoalteromonas sp. PM2 Indigenous from Alor Island, Indonesia." Indonesian Journal of Natural Pigments 3, no. 1 (February 26, 2021): 16–23. http://dx.doi.org/10.33479/ijnp.2021.03.1.16-23.

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Pigments from marine bacteria have attracted the attention for scientists because of their extensive applications and currently exploration of new pigment sources from marine bacteria is still ongoing. Recently, we have successfully isolated six new yellow-pigmented marine bacteria, strain PS2, PM2, SB11, SB13, SB21, and SB23, isolated from seawater from different sampling sites on Alor Island, Indonesia. The UV−Vis and FTIR spectra of the crude pigment extracts of the six strains showed the characteristics of tambjamines, a group of yellow pigments commonly found in nudibranchs and bryozoans. Moreover, separation and characterization of crude tambjamines extract resulted in five different types of tambjamine with maximum absorbance at the wavelength of 374−392 nm. Based on the analysis of 16S rRNA gene sequences, strain PM2 was closely related to several species in genus Pseudoalteromonas with a similarity of more than 99%. Strain PM2 was designed as Pseudoalteromonas sp. PM2 with accession number LC505058. So far, only two marine bacteria have been known to produce tambjamine and they are from genus Pseudoalteromonas. Our new finding indicated that in the group of marine bacteria, tambjamine might be only synthesized by members from genus Pseudoalteromonas.

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Matsuyama, Hidetoshi, Hideki Minami, Hirokazu Kasahara, Yoshihisa Kato, Masafumi Murayama, and Isao Yumoto. "Pseudoalteromonas arabiensis sp. nov., a marine polysaccharide-producing bacterium." International Journal of Systematic and Evolutionary Microbiology 63, Pt_5 (May 1, 2013): 1805–9. http://dx.doi.org/10.1099/ijs.0.043604-0.

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A novel exopolysaccharide-producing bacterium, designated strain k53T, was isolated from sediment from the Arabia Sea, Indian Ocean. The strain was Gram-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na+ for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The DNA G+C content was 43 mol%. 16S rRNA gene sequence analysis suggested that strain k53T is a member of the genus Pseudoalteromonas . Strain k53T exhibited close phylogenetic affinity to Pseudoalteromonas lipolytica LMEB 39T (98.0% 16S rRNA gene sequence similarity) and Pseudoalteromonas donghaensis HJ51T (97.3 %).The DNA–DNA reassociation values between strain k53T and P. lipolytica JCM 15903T and P. donghaensis LMG 24469T were 17 % and 12 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analysis based on the 16S rRNA gene sequence and DNA–DNA relatedness data, the isolate merits classification as a representative of a novel species, for which the name Pseudoalteromonas arabiensis is proposed. The type strain of this species is k53T ( = JCM 17292T = NCIMB 14688T).

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Lau, Stanley C. K., Mandy M. Y. Tsoi, Xiancui Li, Sergey Dobretsov, Yulia Plakhotnikova, Po-Keung Wong, and Pei-Yuan Qian. "Pseudoalteromonas spongiae sp. nov., a novel member of the γ-Proteobacteria isolated from the sponge Mycale adhaerens in Hong Kong waters." International Journal of Systematic and Evolutionary Microbiology 55, no. 4 (July 1, 2005): 1593–96. http://dx.doi.org/10.1099/ijs.0.63638-0.

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A Gram-negative, non-spore-forming, short rod-shaped bacterium (UST010723-006T) was isolated from the surface of the sponge Mycale adhaerens in Hong Kong waters. Cells of UST010723-006T did not have flagella and were non-motile. Colonies were pale orange in colour, 2–4 mm in diameter, convex with a smooth surface and an entire translucent margin. Gas bubbles were observed in the colonies and also in the agar matrix underneath and adjacent to the colonies. UST010723-006T was heterotrophic, strictly aerobic and required NaCl for growth (2·0–6·0 %). It grew at pH 5·0–10·0 and between 12 and 44 °C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST010723-006T within the genus Pseudoalteromonas of the γ-subclass of the Proteobacteria. The DNA G+C content is 40·6 mol% and the dominant fatty acids were 12 : 0 3-OH, 14 : 0, 15 : 0 iso 2-OH, 16 : 0, 16 : 1ω7, 17 : 1ω8 and 18 : 1ω7 (altogether representing 75·9 % of the total).These data supported the affiliation of UST010723-006T to the genus Pseudoalteromonas. The closest relatives were Pseudoalteromonas luteviolacea, P. phenolica, P. rubra and P. ruthenica with similarity values ranging from 95·4 to 96·8 %. UST010723-006T differed from these closest relatives by 9–19 traits. Molecular evidence, together with phenotypic characteristics, suggests that UST010723-006T constitutes a novel species within the genus Pseudoalteromonas. The name Pseudoalteromonas spongiae sp. nov. is proposed for this bacterium. The type strain is UST010723-006T (=NRRL B-41100T=JCM 12884T).

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Andreo-Vidal, Andrés, Antonio Sanchez-Amat, and Jonatan Campillo-Brocal. "The Pseudoalteromonas luteoviolacea L-amino Acid Oxidase with Antimicrobial Activity Is a Flavoenzyme." Marine Drugs 16, no. 12 (December 12, 2018): 499. http://dx.doi.org/10.3390/md16120499.

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The marine environment is a rich source of antimicrobial compounds with promising pharmaceutical and biotechnological applications. The Pseudoalteromonas genus harbors one of the highest proportions of bacterial species producing antimicrobial molecules. For decades, the presence of proteins with L-amino acid oxidase (LAAO) and antimicrobial activity in Pseudoalteromonas luteoviolacea has been known. Here, we present for the first time the identification, cloning, characterization and phylogenetic analysis of Pl-LAAO, the enzyme responsible for both LAAO and antimicrobial activity in P. luteoviolacea strain CPMOR-2. Pl-LAAO is a flavoprotein of a broad substrate range, in which the hydrogen peroxide generated in the LAAO reaction is responsible for the antimicrobial activity. So far, no protein with a sequence similarity to Pl-LAAO has been cloned or characterized, with this being the first report on a flavin adenine dinucleotide (FAD)-containing LAAO with antimicrobial activity from a marine microorganism. Our results revealed that 20.4% of the sequenced Pseudoalteromonas strains (specifically, 66.6% of P. luteoviolacea strains) contain Pl-laao similar genes, which constitutes a well-defined phylogenetic group. In summary, this work provides insights into the biological significance of antimicrobial LAAOs in the Pseudoalteromonas genus and shows an effective approach for the detection of novel LAAOs, whose study may be useful for biotechnological applications.

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Donovan, Carrie J., Rafael A. Garduño, Martin Kalmokoff, John C. Ku, Michael A. Quilliam, and Tom A. Gill. "Pseudoalteromonas Bacteria Are Capable of Degrading Paralytic Shellfish Toxins." Applied and Environmental Microbiology 75, no. 21 (August 28, 2009): 6919–23. http://dx.doi.org/10.1128/aem.01384-09.

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ABSTRACT Marine bacterial isolates cultured from the digestive tracts of blue mussels (Mytilus edulis) contaminated with paralytic shellfish toxins (PSTs) were screened for the ability to reduce the toxicity of a PST mixture. Seven isolates reduced the overall toxicity of the algal extract by ≥90% within 3 days. These isolates shared at least 99% 16S rRNA gene sequence similarity with five Pseudoalteromonas spp. Phenotypic tests suggested that all are novel strains of Pseudoalteromonas haloplanktis.

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Kristiana, Rhesi, Gilles Bedoux, Gerard Pals, I. Wayan Mudianta, Laure Taupin, Christel Marty, Meezan Ardhanu Asagabaldan, et al. "Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia." PeerJ 8 (January 2, 2020): e8093. http://dx.doi.org/10.7717/peerj.8093.

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The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500–1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5–1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant.

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Ivanova, E. P. "Pseudoalteromonas translucida sp. nov. and Pseudoalteromonas paragorgicola sp. nov., and emended description of the genus." INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 52, no. 5 (September 1, 2002): 1759–66. http://dx.doi.org/10.1099/ijs.0.02097-0.

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Ivanova, Elena P., Nataliya M. Gorshkova, Natalia V. Zhukova, Anatolii M. Lysenko, Elena A. Zelepuga, Nina G. Prokof’eva, Valery V. Mikhailov, Dan V. Nicolau, and Richard Christen. "Characterization of Pseudoalteromonas distincta-like sea-water isolates and description of Pseudoalteromonas aliena sp. nov." International Journal of Systematic and Evolutionary Microbiology 54, no. 5 (September 1, 2004): 1431–37. http://dx.doi.org/10.1099/ijs.0.03053-0.

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Seven melanogenic Pseudoalteromonas distincta-like strains, KMM 3562T, KMM 3536, KMM 3537, KMM 3538, KMM 3539, KMM 3615 and KMM 3629, which expressed tyrosinases were isolated from sea-water samples collected from different locations in Amursky Bay (Sea of Japan, Pacific Ocean) and characterized to clarify their taxonomic position. By 16S rRNA gene sequence analysis, the bacteria were shown to belong to the genus Pseudoalteromonas. The G+C content of the DNAs of the strains was 41–43 mol%. The level of DNA similarity among these strains was conspecific (92–97 %), indicating that they represented a single genospecies. However, DNA from the strains isolated from sea water showed only 63–65 % genetic relatedness with the DNA of the type strain P. distincta. The novel organisms grew mainly between 4 and 30 °C, were neutrophilic and slightly halophilic (four strains had a narrow range of growth between 3 and 6 % NaCl, w/v), were haemolytic and cytotoxic and were able to degrade starch, gelatin and Tween 80. The predominant fatty acids, including 16 : 0, 16 : 1ω7, 17 : 1ω7 and 18 : 1ω7, were typical of the genus Pseudolateromonas. The phylogenetic, genetic and physiological properties of the seven strains placed them within a novel species, Pseudoalteromonas aliena sp. nov., the type strain of which is SW19T (=KMM 3562T=LMG 22059T).

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Ivanova, Elena P., Tomoo Sawabe, Anatolii M. Lysenko, Nataliya M. Gorshkova, K. Hayashi, Natalia V. Zhukova, Dan V. Nicolau, Richard Christen, and Valery V. Mikhailov. "Pseudoalteromonas translucida sp. nov. and Pseudoalteromonas paragorgicola sp. nov., and emended description of the genus." International Journal of Systematic and Evolutionary Microbiology 52, no. 5 (September 1, 2002): 1759–66. http://dx.doi.org/10.1099/00207713-52-5-1759.

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Kallies, René, Bärbel Kiesel, Matthias Schmidt, Nawras Ghanem, Jakob Zopfi, Jörg Hackermüller, Hauke Harms, Lukas Y. Wick, and Antonis Chatzinotas. "Complete genome sequence of Pseudoalteromonas virus vB_PspP-H6/1 that infects Pseudoalteromonas sp. strain H6." Marine Genomics 47 (October 2019): 100667. http://dx.doi.org/10.1016/j.margen.2019.03.002.

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Dheilly, Alexandra, Emmanuelle Soum-SoutÃ¯Â¿Â½ra, GÃ¯Â¿Â½raldine L. Klein, Alexis Bazire, Chantal CompÃ¯Â¿Â½re, Dominique Haras, and Alain Dufour. "Antibiofilm Activity of the Marine Bacterium Pseudoalteromonas sp. Strain 3J6." Applied and Environmental Microbiology 76, no. 11 (April 2, 2010): 3452–61. http://dx.doi.org/10.1128/aem.02632-09.

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ABSTRACT Biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. In two-species biofilms of marine bacteria grown under dynamic conditions, Pseudoalteromonas sp. strain 3J6 formed mixed biofilms with Bacillus sp. strain 4J6 but was largely predominant over Paracoccus sp. strain 4M6 and Vibrio sp. strain D01. The supernatant of Pseudoalteromonas sp. 3J6 liquid culture (SN3J6) was devoid of antibacterial activity against free-living Paracoccus sp. 4M6 and Vibrio sp. D01 cells, but it impaired their ability to grow as single-species biofilms and led to higher percentages of nonviable cells in 48-h biofilms. Antibiofilm molecules of SN3J6 were able to coat the glass surfaces used to grow biofilms and reduced bacterial attachment about 2-fold, which might partly explain the biofilm formation defect but not the loss of cell viability. SN3J6 had a wide spectrum of activity since it affected all Gram-negative marine strains tested except other Pseudoalteromonas strains. Biofilm biovolumes of the sensitive strains were reduced 3- to 530-fold, and the percentages of nonviable cells were increased 3- to 225-fold. Interestingly, SN3J6 also impaired biofilm formation by three strains belonging to the human-pathogenic species Pseudomonas aeruginosa, Salmonella enterica, and Escherichia coli. Such an antibiofilm activity is original and opens up a variety of applications for Pseudoalteromonas sp. 3J6 and/or its active exoproducts in biofilm prevention strategies.

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Kivelä, Hanna M., Stefania Madonna, Mart Krupovìč, M. Luisa Tutino, and Jaana K. H. Bamford. "Genetics for Pseudoalteromonas Provides Tools To Manipulate Marine Bacterial Virus PM2." Journal of Bacteriology 190, no. 4 (December 14, 2007): 1298–307. http://dx.doi.org/10.1128/jb.01639-07.

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ABSTRACT The genetic manipulation of marine double-stranded DNA (dsDNA) bacteriophage PM2 (Corticoviridae) has been limited so far. The isolation of an autonomously replicating DNA element of Pseudoalteromonas haloplanktis TAC125 and construction of a shuttle vector replicating in both Escherichia coli and Pseudoalteromonas enabled us to design a set of conjugative shuttle plasmids encoding tRNA suppressors for amber mutations. Using a host strain carrying a suppressor plasmid allows the introduction and analysis of nonsense mutations in PM2. Here, we describe the isolation and characterization of a suppressor-sensitive PM2 sus2 mutant deficient in the structural protein P10. To infect and replicate, PM2 delivers its 10-kbp genome across the cell envelopes of two gram-negative Pseudoalteromonas species. The events leading to the internalization of the circular supercoiled dsDNA are puzzling. In a poorly understood process that follows receptor recognition, the virion capsid disassembles and the internal membrane fuses with the host outer membrane. While beginning to unravel the mechanism of this process, we found that protein P10 plays an essential role in the host cell penetration.

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Sonnenberg, Cecilie Bækkedal, and Peik Haugen. "Bipartite Genomes in Enterobacterales: Independent Origins of Chromids, Elevated Openness and Donors of Horizontally Transferred Genes." International Journal of Molecular Sciences 24, no. 5 (February 21, 2023): 4292. http://dx.doi.org/10.3390/ijms24054292.

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Multipartite bacteria have one chromosome and one or more chromid. Chromids are believed to have properties that enhance genomic flexibility, making them a favored integration site for new genes. However, the mechanism by which chromosomes and chromids jointly contribute to this flexibility is not clear. To shed light on this, we analyzed the openness of chromosomes and chromids of the two bacteria, Vibrio and Pseudoalteromonas, both which belong to the Enterobacterales order of Gammaproteobacteria, and compared the genomic openness with that of monopartite genomes in the same order. We applied pangenome analysis, codon usage analysis and the HGTector software to detect horizontally transferred genes. Our findings suggest that the chromids of Vibrio and Pseudoalteromonas originated from two separate plasmid acquisition events. Bipartite genomes were found to be more open compared to monopartite. We found that the shell and cloud pangene categories drive the openness of bipartite genomes in Vibrio and Pseudoalteromonas. Based on this and our two recent studies, we propose a hypothesis that explains how chromids and the chromosome terminus region contribute to the genomic plasticity of bipartite genomes.

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Park, Sooyeon, Seo Yeon Lee, Wonyong Kim, and Jung-Hoon Yoon. "Pseudoalteromonas caenipelagi sp. nov., isolated from a tidal flat." International Journal of Systematic and Evolutionary Microbiology 70, no. 12 (December 1, 2020): 6301–6. http://dx.doi.org/10.1099/ijsem.0.004532.

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A Gram-stain-negative, aerobic, non-spore-forming, motile by single polar flagellum and ovoid or rod-shaped bacterial strain, designated JBTF-M23T, was isolated from tidal flat sediment collected from the Yellow Sea, Republic of Korea. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M23T fell within the clade comprising the type strains of Pseudoalteromonas species, clustering with the type strains of P. byunsanensis and P. amylolytica . Strain JBTF-M23T exhibited the highest 16S rRNA gene sequence similarity value (98.6 %) to the type strain of P. rubra and sequence similarities of 98.3 and 97.7 % to the type strains of P. byunsanensis and P. amylolytica, respectively. The DNA G+C content of strain JBTF-M23T from genomic sequence data was 41.98 %. The ANI and dDDH values between strain JBTF-M23T and the type strains of P. rubra , P. byunsanensis and P. amylolytica were 71.3–76.6 and 19.4–19.9 %, respectively. Strain JBTF-M23T contained Q-8 as the predominant ubiquinone and C16 : 1 ω7c and/or C16 : 1 ω6c, C16 : 0 and C18 : 1 ω7c as the major fatty acids. The major polar lipids of strain JBTF-M23T were phosphatidylethanolamine and one unidentified aminolipid. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain JBTF-M23T is separated from recognized Pseudoalteromonas species. On the basis of the data presented, strain JBTF-M23Tis considered to represent a novel species of the genus Pseudoalteromonas , for which the name Pseudoalteromonas caenipelagi sp. nov. is proposed. The type strain is JBTF-M23T(=KACC 19900T=NBRC 113647T).

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Patrauchan, M. A., S. Sarkisova, K. Sauer, and M. J. Franklin. "Calcium influences cellular and extracellular product formation during biofilm-associated growth of a marine Pseudoalteromonas sp." Microbiology 151, no. 9 (September 1, 2005): 2885–97. http://dx.doi.org/10.1099/mic.0.28041-0.

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Bacteria undergo a variety of physiological changes following a switch from planktonic growth to surface-associated biofilm growth. Here, it is shown that biofilm development of a marine isolate, Pseudoalteromonas sp. 1398, results in global changes in its cytosolic and extracellular proteomes. Calcium influences these proteome responses, and affects the amount of surface-associated biomass and extracellular matrix material produced by Pseudoalteromonas sp. 1398. Four extracellular proteins, characterized by N-terminal sequencing, showed increased abundances, while one protein, flagellin, showed reduced abundance at higher [Ca2+]. Immunoblotting and transmission-electron-microscopy analysis confirmed that higher [Ca2+] and surface-associated growth results in the repression of flagella production. Two-dimensional gel electrophoresis (2DGE) studies combined with cluster analysis of global proteome responses demonstrated that Ca2+ had a greater regulatory influence on Pseudoalteromonas sp. growing in biofilms than on planktonic cultures. Approximately 22 % of the total cytosolic proteins resolved by 2DGE had differing abundances in response to a switch from planktonic growth to surface-associated growth when the cells were cultivated in 1 mM Ca2+. At higher [Ca2+] this number increased to 38 %. Fifteen cellular proteins that were differentially expressed in response to biofilm growth and/or Ca2+ were analysed by N-terminal sequencing and/or MS/MS. These proteins were identified as factors involved in cellular metabolic functions, putative proteases and transport proteins, although there were several proteins that had not been previously characterized. These results indicate that Ca2+ causes global changes in matrix material, as well as in cellular and extracellular protein profiles of Pseudoalteromonas sp. 1398. These changes are more pronounced when the bacterium grows in biofilms than when it grows in planktonic culture.

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Garrido, Anette, Librada A. Atencio, Rita Bethancourt, Ariadna Bethancourt, Héctor Guzmán, Marcelino Gutiérrez, and Armando A. Durant-Archibold. "Antibacterial Activity of Volatile Organic Compounds Produced by the Octocoral-Associated Bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327." Antibiotics 9, no. 12 (December 18, 2020): 923. http://dx.doi.org/10.3390/antibiotics9120923.

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The present research aimed to evaluate the antibacterial activity of volatile organic compounds (VOCs) produced by octocoral-associated bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327. The volatilome bioactivity of both bacteria species was evaluated against human pathogenic antibiotic-resistant bacteria, methicillin-resistant Staphylococcus aureus, Acinetobacter baumanni, and Pseudomonas aeruginosa. In this regard, the in vitro tests showed that Bacillus sp. BO53 VOCs inhibited the growth of P. aeruginosa and reduced the growth of S. aureus and A. baumanni. Furthermore, Pseudoalteromonas sp. GA327 strongly inhibited the growth of A. baumanni, and P. aeruginosa. VOCs were analyzed by headspace solid-phase microextraction (HS-SPME) joined to gas chromatography-mass spectrometry (GC-MS) methodology. Nineteen VOCs were identified, where 5-acetyl-2-methylpyridine, 2-butanone, and 2-nonanone were the major compounds identified on Bacillus sp. BO53 VOCs; while 1-pentanol, 2-butanone, and butyl formate were the primary volatile compounds detected in Pseudoalteromonas sp. GA327. We proposed that the observed bioactivity is mainly due to the efficient inhibitory biochemical mechanisms of alcohols and ketones upon antibiotic-resistant bacteria. This is the first report which describes the antibacterial activity of VOCs emitted by octocoral-associated bacteria.

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Atencio, Librada A., Cristopher A. Boya P., Christian Martin H., Luis C. Mejía, Pieter C. Dorrestein, and Marcelino Gutiérrez. "Genome Mining, Microbial Interactions, and Molecular Networking Reveals New Dibromoalterochromides from Strains of Pseudoalteromonas of Coiba National Park-Panama." Marine Drugs 18, no. 9 (September 3, 2020): 456. http://dx.doi.org/10.3390/md18090456.

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The marine bacterial genus Pseudoalteromonas is known for their ability to produce antimicrobial compounds. The metabolite-producing capacity of Pseudoalteromonas has been associated with strain pigmentation; however, the genomic basis of their antimicrobial capacity remains to be explained. In this study, we sequenced the whole genome of six Pseudoalteromonas strains (three pigmented and three non-pigmented), with the purpose of identifying biosynthetic gene clusters (BGCs) associated to compounds we detected via microbial interactions along through MS-based molecular networking. The genomes were assembled and annotated using the SPAdes and RAST pipelines and mined for the identification of gene clusters involved in secondary metabolism using the antiSMASH database. Nineteen BGCs were detected for each non-pigmented strain, while more than thirty BGCs were found for two of the pigmented strains. Among these, the groups of genes of nonribosomal peptide synthetases (NRPS) that code for bromoalterochromides stand out the most. Our results show that all strains possess BGCs for the production of secondary metabolites, and a considerable number of distinct polyketide synthases (PKS) and NRPS clusters are present in pigmented strains. Furthermore, the molecular networking analyses revealed two new molecules produced during microbial interactions: the dibromoalterochromides D/D’ (11–12).

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Song, Won-Suk, Seong-Min Kim, Sung-Hyun Jo, Jae-Seung Lee, Hyo-Jin Jeon, Byoung Joon Ko, Kwon-Young Choi, Yung-Hun Yang, and Yun-Gon Kim. "Multi-omics characterization of the osmotic stress resistance and protease activities of the halophilic bacterium Pseudoalteromonas phenolica in response to salt stress." RSC Advances 10, no. 40 (2020): 23792–800. http://dx.doi.org/10.1039/d0ra04034g.

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Mazzotta, Michael G., Matthew R. McIlvin, and Mak A. Saito. "Characterization of the Fe metalloproteome of a ubiquitous marine heterotroph, Pseudoalteromonas (BB2-AT2): multiple bacterioferritin copies enable significant Fe storage." Metallomics 12, no. 5 (2020): 654–67. http://dx.doi.org/10.1039/d0mt00034e.

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Chellaram, C., T. Prem Anand, C. Felicia Shanthini, B. Arvind Kumar, and Siddath P. Sarma. "Bioactive Peptides from Epibiotic Pseudoalteromonas Strain P1." APCBEE Procedia 2 (2012): 37–42. http://dx.doi.org/10.1016/j.apcbee.2012.06.008.

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Yu, Zi-Chao, Bai-Lu Tang, Dian-Li Zhao, Xiuhua Pang, Qi-Long Qin, Bai-Cheng Zhou, Xi-Ying Zhang, Xiu-Lan Chen, and Yu-Zhong Zhang. "Development of a Cold-Adapted Pseudoalteromonas Expression System for the Pseudoalteromonas Proteins Intractable for the Escherichia coli System." PLOS ONE 10, no. 9 (September 2, 2015): e0137384. http://dx.doi.org/10.1371/journal.pone.0137384.

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CASTRO, Arizaldo E., and Cristina C. SALIBAY. "Anti-Methicillin Resistant Staphylococcus aureus (MRSA) Activity of Pseudoalteromonas flavipulchra Isolated from Marine Waters of Batangas, Philippines." Walailak Journal of Science and Technology (WJST) 17, no. 6 (June 1, 2020): 570–78. http://dx.doi.org/10.48048/wjst.2020.4789.

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The ocean boasts untapped novel producers of antibiotic substances in the form of unicellular organisms. One of the newest bioproducers of pharmacologically-significant substances studied for its potential against clinically-significant pathogens is the genus Pseudoalteromonas, a gammaproteobacterial group. This study is a preliminary report detailing the isolation of a Pseudoalteromonas flavipulchra species from Philippine marine waters. The isolate coded as PAM-003 was identified as 100 % similar to P. flavipulchra strain NCIMB2033 through 16s rRNA gene amplification and sequencing. PAM-003 was allowed to produce bioactives for 12 days. Afterwards, non-polar products were isolated from the base medium through membrane filtration, organic solvent extraction and rotary evaporation. The crude solution of bioactives injected in sterile discs was used for disc-diffusion assay against Methicillin Resistant Staphylococcus aureus (MRSA). Results indicate that PAM-003 demonstrated visually-appreciable zones of inhibition with a mean value of 8 mm. To further describe the antibacterial activity of the isolate, minimum inhibitory concentration (MIC) of the bacterial extract was determined through broth microdilution technique. Results indicate that PAM-003 demonstrated a MIC of 1000 µg/mL against MRSA. Additional investigation on the bioactivity of Philippine isolates from the genus Pseudoalteromonas isolated from highly diverse regions of the country is a considerable initiative for increasing the pipeline of new molecular entities for drug discovery.

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Pernthaler, Annelie, Jakob Pernthaler, Heike Eilers, and Rudolf Amann. "Growth Patterns of Two Marine Isolates: Adaptations to Substrate Patchiness?" Applied and Environmental Microbiology 67, no. 9 (September 1, 2001): 4077–83. http://dx.doi.org/10.1128/aem.67.9.4077-4083.2001.

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ABSTRACT During bottle incubations of heterotrophic marine picoplankton, some bacterial groups are conspicuously favored. In an earlier investigation bacteria of the genusPseudoalteromonas rapidly multiplied in substrate-amended North Sea water, whereas the densities ofOceanospirillum changed little (H. Eilers, J. Pernthaler, and R. Amann, Appl. Environ. Microbiol. 66:4634–4640, 2000). We therefore studied the growth patterns of two isolates affiliating withPseudoalteromonas and Oceanospirillum in batch culture. Upon substrate resupply, Oceanospirillum lagged threefold longer than Pseudoalteromonas but reached more than fivefold-higher final cell density and biomass. A second, mobile morphotype was present in the starved Oceanospirillumpopulations with distinctly greater cell size, DNA and protein content, and 16S rRNA concentration. Contrasting cellular ribosome concentrations during stationary phase suggested basic differences in the growth responses of the two strains to a patchy environment. Therefore, we exposed the strains to different modes of substrate addition. During cocultivation on a single batch of substrates, the final cell densities of Oceanospirillum were reduced three times as much as those Pseudoalteromonas, compared to growth yields in pure cultures. In contrast, the gradual addition of substrates to stationary-phase cocultures was clearly disadvantageous for the Pseudoalteromonas population. Different growth responses to substrate gradients could thus be another facet affecting the competition between marine bacteria and may help to explain community shifts observed during enrichments.

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Beurmann, Silvia, Blake Ushijima, Christina M. Svoboda, Patrick Videau, Ashley M. Smith, Stuart P. Donachie, Greta S. Aeby, and Sean M. Callahan. "Pseudoalteromonas piratica sp. nov., a budding, prosthecate bacterium from diseased Montipora capitata, and emended description of the genus Pseudoalteromonas." International Journal of Systematic and Evolutionary Microbiology 67, no. 8 (August 1, 2017): 2683–88. http://dx.doi.org/10.1099/ijsem.0.001995.

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Ivanova, Elena P., Sébastien Flavier, and Richard Christen. "Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov." International Journal of Systematic and Evolutionary Microbiology 54, no. 5 (September 1, 2004): 1773–88. http://dx.doi.org/10.1099/ijs.0.02997-0.

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The phylogenetic relationships among marine Alteromonas-like bacteria of the genera Alteromonas, Pseudoalteromonas, Glaciecola, Thalassomonas, Colwellia, Idiomarina, Oceanimonas, Oceanisphaera, Shewanella, Moritella, Ferrimonas, Psychromonas and several other genera of the ‘Gammaproteobacteria’ were studied. Results of 16S rRNA gene sequence analyses revealed that some members of these genera formed several coherent groups at the family level. Characteristic signature oligonucleotides for studied taxa were defined. Signature positions are divided into three classes: (i) single compensatory mutations, (ii) double compensatory mutations and (iii) mutations affecting nucleotides not paired in the secondary structure. The 16S rRNA gene sequence similarity level within genera was 93 % or above. This value can be a useful additional criterion for genus discrimination. On the basis of this work and previous polyphasic taxonomic studies, the circumscription of the family Alteromonadaceae is limited to the genera Alteromonas and Glaciecola and the creation is proposed of the families Pseudoalteromonadaceae fam. nov. to accommodate bacteria of the genera Pseudoalteromonas and Algicola gen. nov. (formerly Pseudoalteromonas bacteriolytica) and Colwelliaceae fam. nov. to accommodate bacteria of the genera Colwellia and Thalassomonas. Bacteria of the genera Oceanimonas and Oceanisphaera formed a robust cluster and shared common signature oligonucleotides. Because of deep branching and lack of association with any other genus, the following families are proposed that include single genera: Idiomarinaceae fam. nov., Psychromonadaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov. and Shewanellaceae fam. nov. Finally, this study also revealed that [Hyphomicrobium] indicum should be reclassified as Photobacterium indicum comb. nov.

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Gutierrez, Tony, Tracy Shimmield, Cheryl Haidon, Kenny Black, and David H. Green. "Emulsifying and Metal Ion Binding Activity of a Glycoprotein Exopolymer Produced by Pseudoalteromonas sp. Strain TG12." Applied and Environmental Microbiology 74, no. 15 (June 13, 2008): 4867–76. http://dx.doi.org/10.1128/aem.00316-08.

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ABSTRACT In this study, we describe the isolation and characterization of a new exopolymer that exhibits high emulsifying activities against a range of oil substrates and demonstrates a differential capacity to desorb various mono-, di-, and trivalent metal species from marine sediment under nonionic and seawater ionic-strength conditions. This polymer, PE12, was produced by a new isolate, Pseudoalteromonas sp. strain TG12 (accession number EF685033), during growth in a modified Zobell's 2216 medium amended with 1% glucose. Chemical and chromatographic analysis showed it to be a high-molecular-mass (>2,000 kDa) glycoprotein composed of carbohydrate (32.3%) and protein (8.2%). PE12 was notable in that it contained xylose as the major sugar component at unusually high levels (27.7%) not previously reported for a Pseudoalteromonas exopolymer. The polymer was shown to desorb various metal species from marine sediment—a function putatively conferred by its high content of uronic acids (28.7%). Seawater ionic strength (simulated using 0.6 M NaCl), however, caused a significant reduction in PE12's ability to desorb the sediment-adsorbed metals. These results demonstrate the importance of electrolytes, a physical parameter intrinsic of seawater, in influencing the interaction of microbial exopolymers with metal ions. In summary, PE12 may represent a new class of Pseudoalteromonas exopolymer with a potential for use in biotechnological applications as an emulsifying or metal-chelating agent. In addition to the biotechnological potential of these findings, the ecological aspects of this and related bacterial exopolymers in marine environments are also discussed.

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Beardsley, Christine, Jakob Pernthaler, Werner Wosniok, and Rudolf Amann. "Are Readily Culturable Bacteria in Coastal North Sea Waters Suppressed by Selective Grazing Mortality?" Applied and Environmental Microbiology 69, no. 5 (May 2003): 2624–30. http://dx.doi.org/10.1128/aem.69.5.2624-2630.2003.

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ABSTRACT We studied the growth of six culturable bacterial lineages from coastal North Sea picoplankton in environmental samples under different incubation conditions. The grazing pressure of heterotrophic nanoflagellates (HNF) was reduced either by double prefiltration through 0.8-μm-pore-size filters or by 10-fold dilutions with 0.2-μm (pore-size) prefiltered seawater. We hypothesized that those γ-proteobacterial genera that are rapidly enriched would also be most strongly affected by HNF regrowth. In the absence of HNF, the mean protein content per bacterial cell increased in both treatments compared to environmental samples, whereas the opposite trend was found in incubations of unaltered seawater. Significant responses to the experimental manipulations were observed in Alteromonas, Pseudoalteromonas, and Vibrio populations. No treatment-specific effects could be detected for members of the Roseobacter group, the Cytophaga latercula-C. marinoflava lineage, or the NOR5 clade. Statistical analysis confirmed a transient increase in the proportions of Alteromonas, Pseudoalteromonas, and Vibrio cells at reduced HNF densities only, followed by an overproportional decline during the phase of HNF regrowth. Cells from these genera were significantly larger than the community average in the dilution treatments, and changes in their relative abundances were negatively correlated with HNF densities. Our findings suggest that bacteria affiliated with frequently isolated genera such as Alteromonas, Pseudoalteromonas, and Vibrio might be rare in coastal North Sea picoplankton because their rapid growth response to changing environmental conditions is counterbalanced by a higher grazing mortality.

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Hwang, Chung Yeon, Byung Cheol Cho, Jin Kyeong Kang, Jihye Park, and Stephen C. Hardies. "Genomic Analysis of Two Cold-Active Pseudoalteromonas Phages Isolated from the Continental Shelf in the Arctic Ocean." Viruses 15, no. 10 (October 7, 2023): 2061. http://dx.doi.org/10.3390/v15102061.

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Cold-active bacteriophages are bacterial viruses that infect and replicate at low temperatures (≤4 °C). Understanding remains limited of how cold-active phage–host systems sustain high viral abundance despite the persistently low temperatures in pelagic sediments in polar seas. In this study, two Pseudoalteromonas phages, ACA1 and ACA2, were isolated from sediment core samples of the continental shelf in the western Arctic Ocean. These phages exhibited successful propagation at a low temperature of 1 °C and displayed typical myovirus morphology with isometric icosahedral heads and contractile tails. The complete genome sequences of phages ACA1 and ACA2 were 36,825 bp and 36,826 bp in size, respectively, sharing almost the same gene content. These are temperate phages encoding lysogeny-related proteins such as anti-repressor, immunity repressor and integrase. The absence of cross-infection between the host strains, which were genomically distinct Pseudoalteromonas species, can likely be attributed to heavy divergence in the anti-receptor apparently mediated by an associated diversity-generating retroelement. HHpred searching identified genes for all of the structural components of a P2-like phage (family Peduoviridae), although the whole of the Peduoviridae family appeared to be divided between two anciently diverged tail modules. In contrast, Blast matching and whole genome tree analysis are dominated by a nonstructural gene module sharing high similarity with Pseudoalteromonas phage C5a (founder of genus Catalunyavirus). This study expands the knowledge of diversity of P2-like phages known to inhabit Peudoalteromonas and demonstrates their presence in the Arctic niche.

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Männistö, Riina H., A. Marika Grahn, Dennis H. Bamford, and Jaana K. H. Bamford. "Transcription of Bacteriophage PM2 Involves Phage-Encoded Regulators of Heterologous Origin." Journal of Bacteriology 185, no. 11 (June 1, 2003): 3278–87. http://dx.doi.org/10.1128/jb.185.11.3278-3287.2003.

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ABSTRACT Bacteriophage PM2 is the only described member of the Corticoviridae family. It is an icosahedral dsDNA virus with a membrane residing underneath the protein coat. PM2 infects some gram-negative Pseudoalteromonas spp. In the present study, we mapped the viral promoters and showed that the PM2 genome consists of three operons. Four new virus genes were assigned based on their function in transcription. Proteins P15 and P16 are shown to repress early transcription, and proteins P13 and P14 are shown to activate late transcription events. The early regulatory region, containing genes for proteins P15 and P16, as well as the newly identified early promoter region in PM2, has significant sequence similarity with the Pseudoalteromonas pAS28 plasmid. P14, the transcription activator for the structural genes, has a zinc finger motif homologous to archaeal and eukaryotic TFIIS-type regulatory factors.

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Estiningtyas, Rizki. "Keragaman Bakteri Pendegradasi Polietilena di Hutan Mangrove Ambon." BERITA BIOLOGI 21, no. 3 (December 26, 2022): 221–30. http://dx.doi.org/10.14203/beritabiologi.v21i3.4331.

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Peningkatan sampah plastik di lingkungan merupakan ancaman bagi permukaan bumi apabila tidak dikelola dengan baik. Jenis plastik yang banyak digunakan oleh masyarakat yaitu polietilena. Pengelolaan polietilena dapat dilakukan dengan biodegradasi. Penelitian ini bertujuan mengetahui keragaman bakteri pendegradasi polietilena dari hutan mangrove Ambon. Isolasi bakteri pendegradasi polietilena dilakukan menggunakan media pengayaan dengan penambahan bubuk plastik linear low-density polyethylene (LLDPE) sebagai sumber karbon. Hasil penelitian ini didapatkan sebanyak 26 isolat bakteri pendegradasi polietilena berhasil diisolasi dari tanah mangrove. Berdasarkan analisis 16s rRNA, semua isolat termasuk dalam filum Proteobacteria dengan subfilum gamma proteobacteria dan berasal dari 5 genus, yaitu Microbulbifer, Zobellella, Pseudoalteromonas, Vibrio, dan Pseudomonas. Terdapat 13 spesies teridentifikasi dari ke-26 isolat tersebut, yaitu Microbulbifer pacificus, Microbulbifer okinawensis, Microbulbifer arenaceous, Microbulbifer elongatus, Microbulbifer hydrolyticus, Microbulbifer salipaludis, Zobellella aerophila, Pseudoalteromonas profundi, Vibrio tritonius, Vibrio alginolyticus, Pseudomonas alcaligenes, Pseudomonas marincola, dan Pseudomonas benzenivorans.

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Pentekhina, Iuliia, Olga Nedashkovskaya, Aleksandra Seitkalieva, Vladimir Gorbach, Lubov Slepchenko, Natalya Kirichuk, Anna Podvolotskaya, Oksana Son, Liudmila Tekutyeva, and Larissa Balabanova. "Chitinolytic and Fungicidal Potential of the Marine Bacterial Strains Habituating Pacific Ocean Regions." Microorganisms 11, no. 9 (September 8, 2023): 2255. http://dx.doi.org/10.3390/microorganisms11092255.

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Screening for chitinolytic activity in the bacterial strains from different Pacific Ocean regions revealed that the highly active representatives belong to the genera Microbulbifer, Vibrio, Aquimarina, and Pseudoalteromonas. The widely distributed chitinolytic species was Microbulbifer isolated from the sea urchin Strongylocentrotus intermedius. Among seventeen isolates with confirmed chitinolytic activity, only the type strain P. flavipulchra KMM 3630T and the strains of putatively new species Pseudoalteromonas sp. B530 and Vibrio sp. Sgm 5, isolated from sea water (Vietnam mollusc farm) and the sea urchin S. intermedius (Peter the Great Gulf, the Sea of Japan), significantly suppressed the hyphal growth of Aspergillus niger that is perspective for the biocontrol agents’ development. The results on chitinolytic activities and whole-genome sequencing of the strains under study, including agarolytic type strain Z. galactanivorans DjiT, found the new functionally active chitinase structures and biotechnological potential.

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Higgins, Brian P., Adam C. Popkowski, Peter R. Caruana, and Anna C. Karls. "Site-Specific Insertion of IS492 in Pseudoalteromonas atlantica." Journal of Bacteriology 191, no. 20 (August 14, 2009): 6408–14. http://dx.doi.org/10.1128/jb.00771-09.

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ABSTRACT Reversible insertion of IS492 at a site within epsG on the Pseudoalteromonas atlantica chromosome controls peripheral extracellular polysaccharide production and biofilm formation by P. atlantica. High-frequency precise excision of IS492 from epsG requires 5 and 7 bp of flanking DNA, suggesting that IS492 transposition involves a site-specific recombination mechanism. The site specificity of IS492 insertion was examined in P. atlantica and shown to be specific for a 7-bp target, 5′-CTTGTTA-3′. Characterization of numerous insertion events at the target site in epsG indicated that insertion is also orientation specific. The frequency of IS492 insertion at the epsG target site (2.7 × 10−7/cell/generation), determined by quantitative PCR, is 4 to 5 orders of magnitude lower than the frequency of IS492 precise excision from the same site. Comparison of insertion sites for IS492 and the highly related ISPtu2 from Pseudoalteromonas tunicata suggests DNA sequence and/or structural features that may contribute to site recognition and recombination by the transposase of IS492.

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Kim, Jong-Oh, Seok-Ryel Kim, Jae-Myung Lim, Soo-Wan Nam, and Hyeung-Rak Kim. "Overexpression of Recombinant Arylsulfatase Cloned from Pseudoalteromonas carrageenovora." Fisheries and aquatic sciences 8, no. 3 (September 1, 2005): 118–21. http://dx.doi.org/10.5657/fas.2005.8.3.118.

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Yan, Jing, Yue-Hong Wu, Fan-Xu Meng, Chun-Sheng Wang, Shang-Ling Xiong, Xi-Ying Zhang, Yu-Zhong Zhang, Xue-Wei Xu, and De-Min Zhang. "Pseudoalteromonas gelatinilytica sp. nov., isolated from surface seawater." International Journal of Systematic and Evolutionary Microbiology 66, no. 9 (September 1, 2016): 3538–45. http://dx.doi.org/10.1099/ijsem.0.001224.

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Journal articles on the topic 'Pseudoalteromonas'

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