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Journal articles on the topic 'Pseudechis'

Author: Grafiati

Published: 4 June 2021

Last updated: 9 February 2022

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1

Takasaki, C., and N. Tamiya. "Isolation and amino acid sequence of a short-chain neurotoxin from an Australian elapid snake, Pseudechis australis." Biochemical Journal 232, no. 2 (December 1, 1985): 367–71. http://dx.doi.org/10.1042/bj2320367.

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A short-chain neurotoxin Pseudechis australis a (toxin Pa a) was isolated from the venom of an Australian elapid snake Pseudechis australis (king brown snake) by sequential chromatography on CM-cellulose, Sephadex G-50 and CM-cellulose columns. Toxin Pa a has an LD50 (intravenous) value of 76 micrograms/kg body wt. in mice and consists of 62 amino acid residues. The amino acid sequence of Pa a shows considerable homology with those of short-chain neurotoxins of elapid snakes, especially of true sea snakes.

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2

Moore, Sara W. M., Vikas K. Bhat, Peter R. Flatt, Victor A. Gault, and Stephen McClean. "Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom." International Journal of Peptide Research and Therapeutics 22, no. 2 (November 30, 2015): 211–18. http://dx.doi.org/10.1007/s10989-015-9499-z.

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3

Jansen, Melanie, Monique McLeod, Julian White, and Geoffrey K. Isbister. "Spotted black snake (Pseudechis guttatus) envenoming." Medical Journal of Australia 186, no. 1 (January 2007): 41–42. http://dx.doi.org/10.5694/j.1326-5377.2007.tb00788.x.

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4

Isbister, G. K., M. R. Hooper, R. Dowsett, G. Maw, L. Murray, and J. White. "Collett's snake (Pseudechis colletti) envenoming in snake handlers." QJM: An International Journal of Medicine 99, no. 2 (January 24, 2006): 109–15. http://dx.doi.org/10.1093/qjmed/hcl007.

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5

Mengden, G. A., and M. Fitzgerald. "Captive Breeding and Oviparity in Pseudechis butleri (Serpentes: Elapidae)." Amphibia-Reptilia 8, no. 2 (1987): 165–69. http://dx.doi.org/10.1163/156853887x00423.

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AbstractThe recently described Australian elapid snake Pseudechis butleri is the least well known representative of the genus in terms of basic biology and reproductive mode. This report describes the reproductive behavior, oviparity and female defence of the egg clutch. Ontogenetic colour change and sexual size dimorphism from birth are demonstrated in the offspring. A review of the literature suggests that these conditions are relatively rare amongst elapids.

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6

Hart, Andrew J., Geoffrey K. Isbister, Paul O’Donnell, Nicholas A. Williamson, and Wayne C. Hodgson. "Species differences in the neuromuscular activity of post-synaptic neurotoxins from two Australian black snakes (Pseudechis porphyriacus and Pseudechis colletti)." Toxicology Letters 219, no. 3 (June 2013): 262–68. http://dx.doi.org/10.1016/j.toxlet.2013.03.026.

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7

Viala, Vincent L., Diana Hildebrand, Maria Trusch, Raghuvir K. Arni, Daniel C. Pimenta, Hartmut Schlüter, Christian Betzel, and Patrick J. Spencer. "Pseudechis guttatus venom proteome: Insights into evolution and toxin clustering." Journal of Proteomics 110 (October 2014): 32–44. http://dx.doi.org/10.1016/j.jprot.2014.07.030.

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8

Ponraj, Durairaj, and Ponnampalam Gopalakrishnakone. "Renal lesions in rhabdomyolysis caused by Pseudechis australis snake myotoxin." Kidney International 51, no. 6 (June 1997): 1956–69. http://dx.doi.org/10.1038/ki.1997.267.

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9

Thomson, Donald F. "Notes on Australian Snakes of the Genera Pseudechis and Oxyuranus." Proceedings of the Zoological Society of London 103, no. 4 (August 21, 2009): 855–60. http://dx.doi.org/10.1111/j.1096-3642.1933.tb01629.x.

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10

Goldenberg, Jonathan, Vittoria Cipriani, Timothy N. W. Jackson, Kevin Arbuckle, Jordan Debono, Daniel Dashevsky, Nadya Panagides, et al. "Proteomic and functional variation within black snake venoms (Elapidae: Pseudechis )." Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 205 (February 2018): 53–61. http://dx.doi.org/10.1016/j.cbpc.2018.01.001.

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11

Laing, G., A. Kamiguti, M. Wilkinson, G. Lowe, and D. Theakston. "Characterization of a phospholipase A2 fraction from Pseudechis papuanus venom." Toxicon 33, no. 3 (March 1995): 300. http://dx.doi.org/10.1016/0041-0101(95)99354-6.

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12

Ponraj, D., and P. Gopalakrishnakone. "Morphological characteristics of myoglobinuric renal damage after Pseudechis australis envenomation." Toxicon 34, no. 3 (March 1996): 317. http://dx.doi.org/10.1016/0041-0101(96)80947-0.

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13

Sethi, Moksh, Mark Cook, and Kenneth D. Winkel. "Persistent anosmia and olfactory bulb atrophy after mulga (Pseudechis australis) snakebite." Journal of Clinical Neuroscience 29 (July 2016): 199–201. http://dx.doi.org/10.1016/j.jocn.2015.12.019.

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14

Sanhajariya, Suchaya, Stephen B. Duffull, and Geoffrey K. Isbister. "Investigating myotoxicity following Australian red-bellied black snake (Pseudechis porphyriacus) envenomation." PLOS ONE 16, no. 9 (September 10, 2021): e0256653. http://dx.doi.org/10.1371/journal.pone.0256653.

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Background Myotoxicity is one of the common clinical manifestations of red-bellied black snake (Pseudechis porphyriacus) envenomation characterised by elevated creatine kinase (CK) concentrations of greater than 1000 U/L. This study aimed to investigate the occurrence of myotoxicity in patients following envenomation. Methods/Principal findings Patient characteristics and serial blood samples (timed venom concentrations and CK concentrations, pre- and post- antivenom) from 114 patients (median age 41, 2-90y; 80 male) were extracted from the Australian Snakebite Project database. Patients were categorised into three groups based on peak CK concentrations [no myotoxicity (<1000 U/L), mild (1000–10,000 U/L) and severe (>10,000 U/L)]. The odds of (mild or severe) myotoxicity was lower in patients that received early antivenom (within 6 hours post-bite) compared to those that received late or no antivenom (odd ratio was 0.186; 95% confidence interval, 0.052–0.664). A population pharmacokinetic-pharmacodynamic (PKPD) model was developed to describe the relationship between the time course of venom (a mixture of toxins) and effect (elevated CK). In addition, a kinetic-pharmacodynamic (KPD) model was developed to describe the relationship between time course of a theoretical toxin and effect. Model development and parameter estimation was performed using NONMEM v7.3. No single set of parameter values from either the PKPD or KPD models were found that could accurately describe the time course of different levels of severity of myotoxicity. The predicted theoretical toxin half-life from the KPD model was 11 ± 3.9 hours compared to the half-life of venom of 5.3 ± 0.36 hours. This indicates that the putative causative toxin’s concentration-time profile does not parallel that of venom. Conclusion Early antivenom administration reduces the incidence of myotoxicity. The venom concentration profile does not appear to be the driver for myotoxicity following envenomation. Additional factors that affect the sensitivity of the patient to snake venom/toxins must be explored to understand the relationship with myotoxicity.

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15

Portas, Timothy J., and Richard J. Montali. "SPOTTED BLACK SNAKE (PSEUDECHIS GUTTATUS) ENVENOMATION IN A MANED WOLF (CHRYSOCYON BRACHYURUS)." Journal of Zoo and Wildlife Medicine 38, no. 3 (September 2007): 483–87. http://dx.doi.org/10.1638/1042-7260(2007)38[483:sbspge]2.0.co;2.

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16

Lim, Adeline Y. L., Puneet N. Singh, and Geoffrey K. Isbister. "Severe rhabdomyolysis from red-bellied black snake (Pseudechis porphyriacus) envenoming despite antivenom." Toxicon 117 (July 2016): 46–48. http://dx.doi.org/10.1016/j.toxicon.2016.03.016.

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17

Ryan, Rachael Y. M., Viviana P. Lutzky, Volker Herzig, Taylor B. Smallwood, Jeremy Potriquet, Yide Wong, Paul Masci, et al. "Venom of the Red-Bellied Black Snake Pseudechis porphyriacus Shows Immunosuppressive Potential." Toxins 12, no. 11 (October 26, 2020): 674. http://dx.doi.org/10.3390/toxins12110674.

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Venoms act with remarkable specificity upon a broad diversity of physiological targets. Venoms are composed of proteins, peptides, and small molecules, providing the foundation for the development of novel therapeutics. This study assessed the effect of venom from the red-bellied black snake (Pseudechis porphyriacus) on human primary leukocytes using bead-based flow cytometry, mixed lymphocyte reaction, and cell viability assays. We show that venom treatment had a significant immunosuppressive effect, inhibiting the secretion of interleukin (IL)-2 and tumor necrosis factor (TNF) from purified human T cells by 90% or greater following stimulation with mitogen (phorbol 12-myristate 13-acetate and ionomycin) or via cluster of differentiation (CD) receptors, CD3/CD28. In contrast, venom treatment did not inhibit TNF or IL-6 release from antigen-presenting cells stimulated with lipopolysaccharide. The reduced cytokine release from T cells was not associated with inhibition of T cell proliferation or reduction of cell viability, consistent with an anti-inflammatory mechanism unrelated to the cell cycle. Deconvolution of the venom using reverse-phase HPLC identified four fractions responsible for the observed immunosuppressive activity. These data suggest that compounds from P. porphyriacus venom may be potential drug leads for T cell-associated conditions such as graft versus host disease, rheumatoid arthritis, and inflammatory bowel disease.

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18

Shine, R. "Reproductive mode may determine geographic distributions in Australian venomous snakes (Pseudechis, Elapidae)." Oecologia 71, no. 4 (March 1987): 608–12. http://dx.doi.org/10.1007/bf00379306.

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19

Laing, G. D., A. S. Kamiguti, M. Wilkinson, G. Lowe, and R. D. G. Theakston. "Studies on phospholipase A2 isolated from Papuan black snake (Pseudechis papunaus) venom." Toxicon 33, no. 5 (May 1995): 588. http://dx.doi.org/10.1016/0041-0101(95)90101-9.

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20

Mo, Matthew, Peter Hayler, and Antonia Hayler. "Observations of Australian Red-bellied Blacksnakes (Pseudechis porphyriacus) preying on freshwater eels." Reptiles & Amphibians 27, no. 1 (April 1, 2020): 91–93. http://dx.doi.org/10.17161/randa.v27i1.14471.

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21

Pearn, John, Bruce McGuire, Lynne McGuire, and Peter Richardson. "The envenomation syndrome caused by the Australian Red-bellied Black Snake Pseudechis porphyriacus." Toxicon 38, no. 12 (December 2000): 1715–29. http://dx.doi.org/10.1016/s0041-0101(00)00102-1.

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22

Zdenek, Christina N., Nicholas J. Youngman, Chris Hay, James Dobson, Nathan Dunstan, Luke Allen, Leontina Milanovic, and Bryan G. Fry. "Anticoagulant activity of black snake (Elapidae: Pseudechis) venoms: Mechanisms, potency, and antivenom efficacy." Toxicology Letters 330 (September 2020): 176–84. http://dx.doi.org/10.1016/j.toxlet.2020.05.014.

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23

Lincz, Lisa F., Fiona E. Scorgie, Christopher I. Johnston, Margaret O’Leary, Ritam Prasad, Michael Seldon, Emmanuel Favaloro, and Geoffrey K. Isbister. "Comparative sensitivity of commercially available aPTT reagents to mulga snake (Pseudechis australis) venom." Pathology 46, no. 5 (August 2014): 444–49. http://dx.doi.org/10.1097/pat.0000000000000120.

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24

Hart, Andrew J., Wayne C. Hodgson, Tracey Scott-Davey, and John B. Harris. "Neuromuscular toxicology of the venom of Collett's snake (Pseudechis colletti ): A histopathological study." Muscle & Nerve 43, no. 4 (February 8, 2011): 552–59. http://dx.doi.org/10.1002/mus.21916.

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25

Shine, Richard, and Robert Lambeck. "Seasonal shifts in the thermoregulatory behaviour of australian blacksnakes, Pseudechis porphyriacus (serpentes: elapidae)." Journal of Thermal Biology 15, no. 3-4 (July 1990): 301–5. http://dx.doi.org/10.1016/0306-4565(90)90016-b.

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26

Kelly-Bosma, Mirrim, Ellie Leister, Andrew Padula, Andrea Schaffer-White, Helle Bielefeldt-Ohmann, Mark Haworth, Joerg Henning, and Rachel Allavena. "Pathology of Fatal Australian Black Snake (Pseudechis sp) Envenomation in Two Adult Dogs." Journal of Comparative Pathology 186 (July 2021): 1–6. http://dx.doi.org/10.1016/j.jcpa.2021.04.009.

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27

Shine, Richard. "Intraspecific Variation in Thermoregulation, Movements and Habitat Use by Australian Blacksnakes, Pseudechis porphyriacus (Elapidae)." Journal of Herpetology 21, no. 3 (September 1987): 165. http://dx.doi.org/10.2307/1564479.

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28

HELLER, J., KL BOSWARD, DR HODGSON, and R. POTTIE. "Anuric renal failure in a dog after Red-bellied Black snake (Pseudechis porphyriacus) envenomation." Australian Veterinary Journal 84, no. 5 (May 2006): 158–62. http://dx.doi.org/10.1111/j.1751-0813.2006.tb12769.x.

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29

Georgieva, Dessislava, Jana Seifert, Michaela Öhler, Martin von Bergen, Patrick Spencer, Raghuvir K. Arni, Nicolay Genov, and Christian Betzel. "Pseudechis australisVenomics: Adaptation for a Defense against Microbial Pathogens and Recruitment of Body Transferrin." Journal of Proteome Research 10, no. 5 (May 6, 2011): 2440–64. http://dx.doi.org/10.1021/pr101248e.

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30

Lalloo, David, Andrew Trevett, Julie Black, James Mapao, Sirus Naraqi, Dale Owens, Ron Hutton, R. David G. Theakston, and David A. Warrell. "Neurotoxicity and haemostatic disturbances in patients envenomed by the Papuan black snake (Pseudechis papuanus)." Toxicon 32, no. 8 (August 1994): 927–36. http://dx.doi.org/10.1016/0041-0101(94)90371-9.

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31

Ponraj, D., and P. Gopalakrishnakone. "Ultrastructural changes induced by myotoxin from Pseudechis australis in murine skeletal muscle and nerve." Toxicon 33, no. 6 (June 1995): 719–20. http://dx.doi.org/10.1016/0041-0101(95)97383-y.

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32

Bernheimer, A. W., R. Linder, S. A. Weinstein, and K. S. Kim. "Isolation and characterization of a phospholipase B from venom of collett's snake, Pseudechis colletti." Toxicon 25, no. 5 (January 1987): 547–54. http://dx.doi.org/10.1016/0041-0101(87)90290-x.

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33

Maddock, Simon T., Aaron Childerstone, Bryan Grieg Fry, David J. Williams, Axel Barlow, and Wolfgang Wüster. "Multi-locus phylogeny and species delimitation of Australo-Papuan blacksnakes (Pseudechis Wagler, 1830: Elapidae: Serpentes)." Molecular Phylogenetics and Evolution 107 (February 2017): 48–55. http://dx.doi.org/10.1016/j.ympev.2016.09.005.

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34

Inagaki, Hidetoshi, Yoko Yamauchi, Michihisa Toriba, and Tai Kubo. "Regional divergence of phospholipase A2-like protein cDNAs between New Guinean and Australian Pseudechis australis." Toxicon 56, no. 4 (September 2010): 637–39. http://dx.doi.org/10.1016/j.toxicon.2010.04.018.

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35

Lane, J., M. A. O’Leary, and G. K. Isbister. "Coagulant effects of black snake (Pseudechis spp.) venoms and in vitro efficacy of commercial antivenom." Toxicon 58, no. 3 (September 2011): 239–46. http://dx.doi.org/10.1016/j.toxicon.2011.05.020.

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36

Inagaki, Hidetoshi, Hikari Kimoto, Yoko Yamauchi, Michihisa Toriba, and Tai Kubo. "Functional characterization of Kunitz-type protease inhibitor Pr-mulgins identified from New Guinean Pseudechis australis." Toxicon 59, no. 1 (January 2012): 74–80. http://dx.doi.org/10.1016/j.toxicon.2011.10.005.

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37

Du, Qianyun Sharon, Manuela Trabi, Renée Stirling Richards, Peter Mirtschin, Frank Madaras, Amanda Nouwens, Kong-Nan Zhao, et al. "Characterization and structural analysis of a potent anticoagulant phospholipase A2 from Pseudechis australis snake venom." Toxicon 111 (March 2016): 37–49. http://dx.doi.org/10.1016/j.toxicon.2015.12.017.

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38

Ponraj, D., and P. Gopalakrishnakone. "Glomerular changes after Pseudechis australis envenomalion, a light microscopic, transmission and scanning electron microscopic study." Toxicon 34, no. 6 (June 1996): 623. http://dx.doi.org/10.1016/0041-0101(96)89131-8.

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39

Nishida, Shigeru, and Nobuo Tamiya. "Tryptophan residues of phospholipase A2 from the venom of an Australian elapid snake (Pseudechis australis)." Toxicon 29, no. 4-5 (January 1991): 429–39. http://dx.doi.org/10.1016/0041-0101(91)90017-l.

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40

Herndon, A. M., A. T. Thompson, and C. Mack. "Diagnosis and Treatment of Lower Motor Neuron Disease in Australian Dogs and Cats." Journal of Veterinary Medicine 2018 (August 6, 2018): 1–11. http://dx.doi.org/10.1155/2018/1018230.

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Diseases presenting with lower motor neuron (LMN) signs are frequently seen in small animal veterinary practice in Australia. In addition to the most common causes of LMN disease seen world-wide, such as idiopathic polyradiculoneuritis and myasthenia gravis, there are several conditions presenting with LMN signs that are peculiar to the continent of Australia. These include snake envenomation by tiger (Notechis spp.), brown (Pseudonaja spp.), and black snakes (Pseudechis spp.), tick paralysis associated with Ixodes holocyclus and Ixodes coronatus, and tetrodotoxins from marine animals such as puffer fish (Tetraodontidae spp.) and blue-ring octopus (Hapalochlaena spp.). The wide range of differential diagnoses along with the number of etiological-specific treatments (e.g., antivenin, acetylcholinesterase inhibitors) and highly variable prognoses underscores the importance of a complete physical exam and comprehensive history to aid in rapid and accurate diagnosis of LMN disease in Australian dogs and cats. The purpose of this review is to discuss diagnosis and treatment of LMN diseases seen in dogs and cats in Australia.

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41

Hamer, Rowena, Francis L. Lemckert, and Peter B. Banks. "Adult frogs are sensitive to the predation risks of olfactory communication." Biology Letters 7, no. 3 (January 12, 2011): 361–63. http://dx.doi.org/10.1098/rsbl.2010.1127.

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Olfaction is a common sensory mode of communication in much of the Vertebrata, although its use by adult frogs remains poorly studied. Being part of an open signalling system, odour cues can be exploited by ‘eavesdropping’ predators that hunt by smell, making association with odour a high-risk behaviour for prey. Here, we show that adult great barred frogs ( Mixophes fasciolatus ) are highly attracted to odour cues of conspecifics and those of sympatric striped marsh frogs ( Limnodynastes peronii ). This attraction decreased significantly with the addition of odours of a scent-hunting predator, the red-bellied black snake ( Pseudechis porphyriacus ), indicating that frogs perceived predation risks from associating with frog odours. Male frogs, however, maintained some attraction to unfamiliar conspecific scents even with predator odours present, suggesting that they perceived benefits of odour communication despite the risk. Our results indicate that adult frogs can identify species and individuals from their odours and assess the associated predation risk, revealing a complexity in olfactory communication previously unknown in adult anurans.

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42

Phillips, Ben L., and Richard Shine. "An invasive species induces rapid adaptive change in a native predator: cane toads and black snakes in Australia." Proceedings of the Royal Society B: Biological Sciences 273, no. 1593 (March 21, 2006): 1545–50. http://dx.doi.org/10.1098/rspb.2006.3479.

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Rapid environmental change due to human activities has increased rates of extinction, but some species may be able to adapt rapidly enough to deal with such changes. Our studies of feeding behaviour and physiological resistance to toxins reveal surprisingly rapid adaptive responses in Australian black snakes ( Pseudechis porphyriacus ) following the invasion of a lethally toxic prey item, the cane toad ( Bufo marinus ). Snakes from toad-exposed localities showed increased resistance to toad toxin and a decreased preference for toads as prey. Separate laboratory experiments suggest that these changes are not attributable to learning (we were unable to teach naive snakes to avoid toxic prey) or to acquired resistance (repeated sub-lethal doses did not enhance resistance). These results strongly suggest that black snake behaviour and physiology have evolved in response to the presence of toads, and have done so rapidly. Toads were brought to Australia in 1935, so these evolved responses have occurred in fewer than 23 snake generations.

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43

Padula, Andrew M., and Kenneth D. Winkel. "Red-bellied black snake (Pseudechis porphyriacus) envenomation in the dog: Diagnosis and treatment of nine cases." Toxicon 117 (July 2016): 69–75. http://dx.doi.org/10.1016/j.toxicon.2016.03.022.

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44

Laing, Gavin D., Aura S. Kamiguti, Mark C. Wilkinson, Gordon M. Lowe, and R. David G. Theakston. "Characterisation of a purified phospholipase A2 from the venom of the Papuan black snake (Pseudechis papuanus)." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1250, no. 2 (July 1995): 137–43. http://dx.doi.org/10.1016/0167-4838(95)00081-5.

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45

Schmidt, James J., and John L. Middlebrook. "Purification, sequencing and characterization of pseudexin phospholipases A2 from Pseudechis porphyriacus (Australian red-bellied black snake)." Toxicon 27, no. 7 (January 1989): 805–18. http://dx.doi.org/10.1016/0041-0101(89)90048-2.

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46

Paulling, Kelsey, David Wilson, and Tasmin L. Rymer. "Olfactory recognition of snake cues by fawn-footed mosaic-tailed rats Melomys cervinipes." Behaviour 156, no. 12 (2019): 1235–53. http://dx.doi.org/10.1163/1568539x-00003563.

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Abstract Rodents rely on their sensitive olfactory systems to detect and respond to predators. We investigated the ability of a native Australian rodent, the fawn-footed mosaic-tailed rat Melomys cervinipes, to detect, recognise, and discriminate between two species of native snakes. We used snake sheds from a sympatric venomous red-bellied black snake Pseudechis porphyriacus and a non-sympatric non-venomous Stimson’s python Antaresia stimsoni. 20 mosaic-tailed rats each experienced three olfactory tests using a Y-maze. Rats were first exposed to one snake shed against a paper control, and then exposed to the other snake shed against a paper control. Which rat experienced which shed first was allocated randomly. Mosaic-tailed rats were then exposed to both sheds simultaneously. Rats could detect the snake sheds, spending longer investigating, and making more visits to, the sheds than the paper control. They also recognised the sheds as potentially dangerous, reducing their total investigation over time, but increasing their frequency of visits. However, rats did not discriminate between sheds, suggesting a general strategy for assessing the identity of reptilian predators.

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47

Shine, Richard. "The Evolution of Viviparity: Ecological Correlates of Reproductive Mode within a Genus of Australian Snakes (Pseudechis: Elapidae)." Copeia 1987, no. 3 (August 5, 1987): 551. http://dx.doi.org/10.2307/1445650.

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48

White, Julian, Scott Weinstein, and Sam Alfred. "244. Mulga snake (Pseudechis australis) Bites; A Review of Significant Cases Including an Exceptionally Severe Local Envenoming." Toxicon 60, no. 2 (August 2012): 221–22. http://dx.doi.org/10.1016/j.toxicon.2012.04.245.

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49

Hart, Andrew J., Geoffrey K. Isbister, and Wayne C. Hodgson. "In vitro neurotoxic effects of Pseudechis spp. venoms: A comparison of avian and murine skeletal muscle preparations." Toxicon 63 (March 2013): 112–15. http://dx.doi.org/10.1016/j.toxicon.2012.12.002.

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50

Chen, Samson Z. J., P. Gopalakrishnakone, and Matthew C. E. Gwee. "Pharmacological effects and pathological changes induced by the venom of Pseudechis australis in isolated skeletal muscle preparations." Toxicon 32, no. 3 (March 1994): 303–15. http://dx.doi.org/10.1016/0041-0101(94)90083-3.

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