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Moussavi Nik, Seyyed Hani, Tenielle Porter, Morgan Newman, Benjamin Bartlett, Imran Khan, Miheer Sabale, Melissa Eccles, et al. "Relevance of a Truncated PRESENILIN 2 Transcript to Alzheimer’s Disease and Neurodegeneration." Journal of Alzheimer's Disease 80, no. 4 (April 20, 2021): 1479–89. http://dx.doi.org/10.3233/jad-201133.
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Background: The PRESENILIN genes (PSEN1, PSEN2) encoding for their respective proteins have critical roles in many aspects of Alzheimer’s disease (AD) pathogenesis. The PS2V transcript of PSEN2 encodes a truncated protein and is upregulated in AD brains; however, its relevance to AD and disease progression remains to be determined. Objective: Assess transcript levels in postmortem AD and non-AD brain tissue and in lymphocytes collected under the Australian Imaging Biomarker and Lifestyle (AIBL) study. Methods: Full length PSEN2 and PS2V transcript levels were assessed by quantitative digital PCR in postmortem brain tissue (frontal cortex and hippocampus) from control, AD, frontotemporal dementia (FTD), and Lewy body dementia (LBD). Transcript levels were also assessed in lymphocytes obtained from the Perth subset of the AIBL study (n = 160). Linear regression analysis was used to assess correlations between transcript copy number and brain volume and neocortical amyloid load. Results: PS2V levels increased in AD postmortem brain but PS2V was also present at significant levels in FTD and LBD brains. PS2V transcript was detected in lymphocytes and PS2V/PSEN2 ratios were increased in mild cognitive impairment (p = 0.024) and AD (p = 0.019) groups compared to control group. Increased ratios were significantly correlated with hippocampal volumes only (n = 62, β= –0.269, p = 0.03). Conclusion: Taken together, these results suggest that PS2V may be a marker of overall neurodegeneration.
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Chu, Chishih, Cheng-Hsun Chiu, Chi-Hong Chu, and Jonathan T. Ou. "Nucleotide and Amino Acid Sequences of oriT-traM-traJ-traY-traA-traL Regions and Mobilization of Virulence Plasmids of Salmonella enterica Serovars Enteritidis, Gallinarum-Pullorum, and Typhimurium." Journal of Bacteriology 184, no. 11 (June 1, 2002): 2857–62. http://dx.doi.org/10.1128/jb.184.11.2857-2862.2002.
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ABSTRACT The virulence plasmid of Salmonella enterica serovar Gallinarum-Pullorum (pSPV) but not those of Salmonella enterica serovars Enteritidis (pSEV) and Typhimurium (pSTV) can be readily mobilized by an F or F-like conjugative plasmid. To investigate the reason for the difference, the oriT-traM-traJ-traY-traA-traL regions of the three salmonella virulence plasmids (pSVs) were cloned and their nucleotide and deduced amino acid sequences were examined. The cloned fragments were generally mobilized more readily than the corresponding full-length pSVs, but the recombinant plasmid containing the oriT of pSPV was, as expected, more readily mobilized, with up to 100-fold higher frequency than the recombinant plasmids containing the oriT of the other two pSVs. The nucleotide sequences of the oriT-traM-traJ-traY-traA-traL region of pSEV and pSTV were almost identical (only 4 bp differences), but differed from that of pSPV. Major nucleotide sequence variations were found in traJ, traY, and the Tra protein binding sites sby and sbm. sby of pSPV showed higher similarity than that of pSEV or pSTV to that of the F plasmid. The reverse was true for sbm: similarity was higher with pSEV and pSTV than with pSPV. In the deduced amino acid sequences of the five Tra proteins, major differences were found in TraY: pSEV's TraY was 75 amino acids, pSTV's was 106 amino acids, and pSPV's was 133 amino acids; and there were duplicate consensus βαα fragments in the TraY of pSPV and F plasmid, whereas there was only a single βαα fragment in that of pSEV and pSTV.
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Nishikawa, Atsuko, Takayuki Manabe, Taiichi Katayama, Takashi Kudo, Shinsuke Matsuzaki, Takeshi Yanagita, Hiroaki Okuda, Yoshio Bando, and Masaya Tohyama. "Novel function of PS2V: change in conformation of tau proteins." Biochemical and Biophysical Research Communications 318, no. 2 (May 2004): 435–38. http://dx.doi.org/10.1016/j.bbrc.2004.04.048.
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Ebrahimie, Esmaeil, Seyyed Hani Moussavi Nik, Morgan Newman, Mark Van Der Hoek, and Michael Lardelli. "The Zebrafish Equivalent of Alzheimer’s Disease-Associated PRESENILIN Isoform PS2V Regulates Inflammatory and Other Responses to Hypoxic Stress." Journal of Alzheimer's Disease 52, no. 2 (May 10, 2016): 581–608. http://dx.doi.org/10.3233/jad-150678.
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Gudauskas, R. T., K. B. Burch, P. Jin, A. K. Hagan, and J. R. Weeks. "Identification of Viruses Infecting Peanut in Alabama1." Peanut Science 20, no. 2 (July 1, 1993): 71–73. http://dx.doi.org/10.3146/i0095-3679-20-2-1.
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Abstract Leaf samples collected from 1,883 peanut plants in 158 fields in 14 counties during July-August, 1990 and 1991, were tested for peanut mottle (PMV), peanut stripe (PStV), peanut stunt (PSV), and tomato spotted wilt (TSWV) viruses by sap inoculations onto indicator plants and/or by enzyme-linked immunosorbent assay (ELISA). Of 889 plants showing virus-like symptoms, 58% were infected with TSWV alone or mixed with PMV or PSV, 36% with PMV alone or mixed with TSWV or PSV, and 5% with PSV alone or mixed with TSWV or PMV. Double infections of PMV with TSWV, PSV with TSWV, and PMV with PSV were detected in 24%, 3%, and 1% of the symptomatic plants. Of 994 apparent asymptomatic plants, 16% were infected with PMV alone or mixed with TSWV, 8% with TSWV alone or mixed with PMV or PSV, and 5% with PSV alone or mixed with TSWV. Double infections of PMV with TSWV and PSV with TSWV were detected in 2% and 1% of the asymptomatic plants. PMV and TSWV were found in at least one field in every county sometime during the two seasons; PSV was found in 13 counties. The most notable difference in virus incidence between the two years was that TSWV was found in 20% of the asymptomatic plants in 1990 as compared to 6% in 1991. PStV was not detected by ELISA in 1990 or 1991, and no reactions suggestive of PStV or any virus other than PMV, PSV, or TSWV were observed on indicator plants used to assay the 1990 collections.
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Moussavi Nik, Seyyed Hani, Morgan Newman, Lachlan Wilson, Esmaeil Ebrahimie, Simon Wells, Ian Musgrave, Giuseppe Verdile, Ralph N. Martins, and Michael Lardelli. "Alzheimer's disease-related peptide PS2V plays ancient, conserved roles in suppression of the unfolded protein response under hypoxia and stimulation of γ-secretase activity." Human Molecular Genetics 24, no. 13 (March 26, 2015): 3662–78. http://dx.doi.org/10.1093/hmg/ddv110.
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de Breuil, S., M. S. Nievas, F. J. Giolitti, L. M. Giorda, and S. L. Lenardon. "Occurrence, Prevalence, and Distribution of Viruses Infecting Peanut in Argentina." Plant Disease 92, no. 8 (August 2008): 1237–40. http://dx.doi.org/10.1094/pdis-92-8-1237.
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This is the first survey to determine the occurrence, prevalence, and distribution of peanut (Arachis hypogaea) viral diseases in Argentina. It was conducted in the province of Córdoba, which has 92% of the country's peanut production. It included the main peanut viruses Peanut mottle virus (PeMoV), Peanut stripe virus (PStV), Cucumber mosaic virus (CMV), Peanut stunt virus (PSV), Tomato spotted wilt virus (TSWV), and Groundnut ringspot virus (GRSV). Leaf samples from 1,028 individual peanut plants with virus-like symptoms and 986 samples from asymptomatic plants were collected in six counties of Córdoba over 3 years and serologically tested for the presence of viruses. PeMoV was the most frequently detected virus, found in 58.8, 34.2, and 23.4% of samples from the 2003–04, 2004–05, and 2005–06 growing seasons, respectively, and it was found in all sampled counties. Also, it was the only virus detected in asymptomatic plants. Less than 4% of symptomatic plants were infected with CMV or GRSV; 0.5, 3.6, and 2% of samples were positive for CMV; and 0.5, 3.1, and 1.6% were positive for GRSV in the 2003–04, 2004–05 and 2005–06 seasons, respectively. Some mixed infections were found: CMV-PeMoV and GRSV-PeMoV. During this survey, PSV, PStV, and TSWV were not detected in any peanut samples.
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Schrader, David M. "Dipositronium and other Two-Positronium Compounds." Materials Science Forum 607 (November 2008): 25–29. http://dx.doi.org/10.4028/www.scientific.net/msf.607.25.
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Dipositronium, Ps2, was recently prepared [1]. This is significant because: • It is the first laboratory observation of a molecule that contains more than one positron; • It is the most symmetrical molecule possible; • It is the most non-rigid (floppiest) molecule possible; • The interval between the theoretical establishment of its existence [2] and its laboratory observation [1] is inordinately long – 60 years; and • An extension of the technology developed for the Ps2 observation may soon lead to an observation of the Bose-Einstein condensation of positronium and the development of a gamma ray laser. We briefly discuss the symmetry of Ps2 and how an understanding of it will underlie its characterization in the future. Ps2O and CPs2 might be the next two-positron compounds to be prepared and characterized in the laboratory. A discussion of the contrasting eigenstates of these two molecules is given. An understanding of these states is required in order to identify them.
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Sboeva, Ya, and S. Boronnikova. "Genetic Structure and Interpopulation Differentiation of Eight Pinus sylvestris L. Populations in the Eastern European Plain." Bulletin of Science and Practice 5, no. 12 (December 15, 2019): 89–97. http://dx.doi.org/10.33619/2414-2948/49/10.
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A study of the genetic structure and differentiation of eight populations of Pinus sylvestris L. on the Russian Plain showed that the populations of PsI and PsII (D=0.066) are located at the smallest genetic distance, and between the populations of PsI and PsIV (D=0.308) at the greatest genetic distance. On the dendrogram, the studied populations formed four clusters: PsI and PsII; PsIII and PsIV; PsV and PsVI; PsVII and PsVIII. Analysis of the genetic structure of eight populations of P. sylvestris showed that the expected proportion of heterozygous genotypes (HT) for the total sample was 0.320, the expected proportion of heterozygous genotypes in a single population for all loci (HS) was 0.170, therefore, the population subdivision (GST) was high and amounted to 0.468. The studied populations are highly differentiated, since the interpopulation component accounts for 46.8% of the genetic diversity. In all studied populations, the indicator h has values less than 0.3. An analysis of the fraction of rare alleles showed that the genetic structure is less balanced in the populations PsIII (h=0.254) and PsIV (h=0.273). The most balanced genetic structure in the populations of PsVII (h=0.112) and PsVIII (h=0.127). Data on the genetic structure and differentiation of the common pine populations should be taken into account when developing recommendations for preserving their gene pools.
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Saito, Shiro L. "Stability of atomic systems containing two positrons, Ps2H+, Ps2F+, and Ps2Cl+: A multireference configuration interaction approach." Chemical Physics Letters 419, no. 4-6 (February 2006): 589–94. http://dx.doi.org/10.1016/j.cplett.2005.12.030.
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Hung, Huynh. "Suppression of ps20 Expression in the Rat Uterus by Tamoxifen and Estrogens." Endocrinology 146, no. 5 (May 1, 2005): 2388–96. http://dx.doi.org/10.1210/en.2004-1525.
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Abstract Using differential display methodology, we isolated a tamoxifen-regulated cDNA. This cDNA was identical to the ps20 cDNA isolated from urogenital sinus mesenchymal cells. ps20 expression was detected in various female rat tissues, with the highest expression in lung and heart. ps20 transcripts were low during estrus and proestrus, but high during the diestrous stage of the estrous cycle coincident with estrogen-induced uterine cell proliferation. Treatment of ovary-intact or ovariectomized rats with estrogens or tamoxifen resulted in increased uterine weight and decreased ps20 expression. Uterine involution associated with ovariectomy or antiestrogen treatment led to up-regulation of ps20. Antibody against rat ps20 recognized the native rat ps20 in conditioned medium of primary rat uterine cells and stable ps20-transfected MCF-7 cells with molecular masses of approximately 24, 27, and 29 kDa. In primary rat uterine cells, ps20 secretion was enhanced by ICI 182,780, but was inhibited by estrogens and tamoxifen. Immunohistochemistry revealed that ps20 was localized to smooth muscle and luminal epithelial cells as well as the glandular population of uterine tissue. Conditioned medium derived from ps20-transfected MCF-7 cells, but not Escherichia coli recombinant ps20, exhibited mild growth suppression on PC-3 cells. The data indicate that ps20 expression is negatively regulated by estrogens and tamoxifen and suggest that ps20 may function as a mediator of local growth.
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Not Available, Not Available. "Bado PSGV." Psychotherapeut 44, no. 1 (January 15, 1999): 46–50. http://dx.doi.org/10.1007/s002780050147.
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Alvarez, R., J. Reading, D. F. L. King, M. Hayes, P. Easterbrook, F. Farzaneh, S. Ressler, F. Yang, D. Rowley, and A. Vyakarnam. "WFDC1/ps20 Is a Novel Innate Immunomodulatory Signature Protein of Human Immunodeficiency Virus (HIV)-Permissive CD4+ CD45RO+ Memory T Cells That Promotes Infection by Upregulating CD54 Integrin Expression and Is Elevated in HIV Type 1 Infection." Journal of Virology 82, no. 1 (October 17, 2007): 471–86. http://dx.doi.org/10.1128/jvi.00939-07.
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ABSTRACT Understanding why human immunodeficiency virus (HIV) preferentially infects some CD4+ CD45RO+ memory T cells has implications for antiviral immunity and pathogenesis. We report that differential expression of a novel secreted factor, ps20, previously implicated in tissue remodeling, may underlie why some CD4 T cells are preferentially targeted. We show that (i) there is a significant positive correlation between endogenous ps20 mRNA in diverse CD4 T-cell populations and in vitro infection, (ii) a ps20+ permissive cell can be made less permissive by antibody blockade- or small-interference RNA-mediated knockdown of endogenous ps20, and (iii) conversely, a ps20low cell can be more permissive by adding ps20 exogenously or engineering stable ps20 expression by retroviral transduction. ps20 expression is normally detectable in CD4 T cells after in vitro activation and interleukin-2 expansion, and such oligoclonal populations comprise ps20positive and ps20low/negative isogenic clones at an early differentiation stage (CD45RO+/CD25+/CD28+/CD57−). This pattern is altered in chronic HIV infection, where ex vivo CD4+ CD45RO+ T cells express elevated ps20. ps20 promoted HIV entry via fusion and augmented CD54 integrin expression; both of these effects were reversed by anti-ps20 antibody. We therefore propose ps20 to be a novel signature of HIV-permissive CD4 T cells that promotes infection in an autocrine and paracrine manner and that HIV has coopted a fundamental role of ps20 in promoting cell adhesion for its benefit. Disrupting the ps20 pathway may therefore provide a novel anti-HIV strategy.
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Hapsoro, Dwi, Hajrial Aswidinnoor, Rusmilah Suseno, Jumanto Jumanto, and Sudarsono Sudarsono. "INHERITANCE OF RESISTANCE TO PStV IN TRANSGENIC PEANUTS CONTAINING cp PStV GENE." Jurnal Hama dan Penyakit Tumbuhan Tropika 8, no. 1 (November 4, 2011): 31–38. http://dx.doi.org/10.23960/j.hptt.1831-38.
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Inheritance of Resistance to PStV in Transgenic Peanuts Containing cp PStV Gene. We have obtained transgenic peanut lines containing coat protein gene of PStV. To get maximal use of the transgenic character in a breeding program, it is necessary that the transgene is also stably inherited and expressed. This experiment was conducted from June 2002 – January 2005 at Plant Molecular Biology Laboratory, Bogor Agricultural University, and Queensland Agricultural Biotechnology Center, The University of Queensland, Australia. The research aimed (1) to test whether PStV cp transgene was functional in progenies derived from crosses between transgenic peaanut plants containing PStV cp gene and non-transgenic ones and (2) to determine pattern of inheritance of resistance to PStV as a result of PStV cp gene action. Several crosses were made between trangenic peanut cv.Gajah resistant to PStV (T4 generation) and non-transgenic peanut line WS susceptible to PStV. The F1 and F2 populations were mechanically inoculated with PStV two weeks after planting. The experiment showed that all plants in the F1 population were less susceptible to PStV, suggesting that the transgene was partially dominant. Phenotipic segregation in F2 population was not Mendelian with the appearance of quick and slow recovery plants and the number of resistant plants being more than expected. However, the proportion of transgenic and non-transgenic plants followed 3:1 ratio, which was Mendelian.
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Wan, Anmin, Xianming Chen, and Jonathan Yuen. "Races of Puccinia striiformis f. sp. tritici in the United States in 2011 and 2012 and Comparison with Races in 2010." Plant Disease 100, no. 5 (May 2016): 966–75. http://dx.doi.org/10.1094/pdis-10-15-1122-re.
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Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases on wheat in the United States. In 2011, severe wheat stripe rust caused extensive application of fungicides in the western United States, and the disease was more widespread and caused more yield loss in the eastern United States in 2012. In this study, we characterized virulences and identified races of P. striiformis f. sp. tritici by testing the stripe rust samples collected throughout the United States in 2011 and 2012 on a set of 18 Yr single-gene differentials. In 2011, 35 races were identified from 349 viable samples collected from 19 states of the United States and Ontario province of Canada, with PSTv-11 (35.5%), PSTv-37 (12.6%), PSTv-14 (11.8%), PSTv-4 (5.4%), and PSTv-34 (3.4%) as the top five predominant races. In 2012, 23 races were identified from 341 viable samples collected from 24 states of the United States and Ontario of Canada, with PSTv-37 (47.5%), PSTv-11 (11.7%), PSTv-14 (10.0%), PSTv-52 (9.4%), and PSTv-48 (4.4%) as the top five predominant races. Nationally, PSTv-37, PSTv-52, and PSTv-34 were most widely distributed, while PSTv-11, PSTv-14, PSTv-4, and PSTv-48 were mostly detected in the western United States. High frequencies (>80%) were detected for virulences to Yr6, Yr7, Yr8, Yr9, Yr17, Yr27, Yr44, and YrExp2; moderate frequencies (20 to 80%) for virulences to Yr1, Yr43, YrTr1, and YrTye; low frequencies (<10%) for virulences to Yr10, Yr24, Yr32, and YrSP; and virulences to Yr5 and Yr15 were not detected, indicating that these two genes are still effective against the P. striiformis f. sp. tritici population in the United States. Both positive and negative associations were identified between some of the virulences. In total, 55 races identified from 2010 to 2012 in the United States were clustered into two major virulence groups, and dynamics of predominant races and virulence frequencies for the 3 years were presented and discussed. This information is useful for making decisions when screening wheat germplasm for developing stripe-rust-resistant wheat cultivars and managing the disease by growing cultivars with adequate and durable resistance. The severe epidemics and the occurrence of the large number of races in the 3 years indicate that efforts should be made to use diverse resistance genes, especially to combine effective all-stage resistance genes with genes for high-temperature adult-plant resistance.
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Wan, Anmin, and Xianming Chen. "Virulence Characterization of Puccinia striiformis f. sp. tritici Using a New Set of Yr Single-Gene Line Differentials in the United States in 2010." Plant Disease 98, no. 11 (November 2014): 1534–42. http://dx.doi.org/10.1094/pdis-01-14-0071-re.
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Puccinia striiformis f. sp. tritici causes stripe rust (yellow rust) of wheat and is highly variable in virulence toward wheat with race-specific resistance. During 2010, wheat stripe rust was the most widespread in the recorded history of the United States, resulting in large-scale application of fungicides and substantial yield loss. A new differential set with 18 yellow rust (Yr) single-gene lines was established and used to differentiate races of P. striiformis f. sp. tritici, which were named as race PSTv in distinction from the PST races identified in the past. An octal system was used to describe the virulence and avirulence patterns of the PSTv races. From 348 viable P. striiformis f. sp. tritici isolates recovered from a total of 381 wheat and grass stripe rust samples collected in 24 states, 41 races, named PSTv-1 to PSTv-41, were identified using the new set of 18 Yr single-gene differentials, and their equivalent PST race names were determined on the previous set of 20 wheat cultivar differentials. The frequencies and distributions of the races and their virulences were determined. The five most predominant races were PSTv-37 (34.5%), PSTv-11 (17.5%), PSTv-14 (7.2%), PSTv-36 (5.2%), and PSTv-34 (4.9%). PSTv-37 was distributed throughout the country while PSTv-11 and PSTv-14 were almost restricted to states west of the Rocky Mountains. The races had virulence to 0 to 13 of the 18 Yr genes. Frequencies of virulences toward resistance genes Yr6, Yr7, Yr8, Yr9, Yr17, Yr27, Yr43, Yr44, YrTr1, and YrExp2 were high (67.0 to 93.7%); those to Yr1 (32.8%) and YrTye (31.3%) were moderate; and those to Yr10, Yr24, Yr32, and YrSP were low (3.4 to 5.7%). All of the isolates were avirulent to Yr5 and Yr15.
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Li, Chin-Hao, Tai-Ying Chen, Chien-Chen Wu, Shih-Hsuan Cheng, Min-Yu Chang, Wei-Hong Cheng, Shih-Hau Chiu, et al. "Safety Evaluation and Anti-Inflammatory Efficacy of Lacticaseibacillus paracasei PS23." International Journal of Molecular Sciences 24, no. 1 (December 31, 2022): 724. http://dx.doi.org/10.3390/ijms24010724.
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Lacticaseibacillus paracasei strain PS23 (PS23) exhibits some probiotic properties. In this study, a genomic analysis of PS23 revealed no genes related to virulence or antibiotic resistance. Moreover, ornithine decarboxylase activity was not detected in vitro. In addition, PS23 was sensitive to the tested antibiotics. Genotoxicity tests for PS23 including the Ames test and chromosomal aberrations in vitro using Chinese hamster ovary cells and micronuclei in immature erythrocytes of ICR mice were all negative. Moreover, following a 28-day study involving repeated oral dose toxicity tests (40, 400, and 4000 mg/kg equal 1.28 × 1010, 1.28 × 1011, and 1.28 × 1012 CFU/kg body weight, respectively) using an ICR mouse model, no adverse effects were observed from any doses. In addition, supplementation with live or heat-killed PS23 ameliorates DSS-induced colonic inflammation in mice. Our findings suggest that PS23 is safe and has anti-inflammatory effects and may therefore have therapeutic implications.
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Culver, J. N., J. L. Sherwood, and M. R. Sanborn. "Use of Monoclonal Antibodies in Detection and Serological Classification of Peanut Stripe Virus1." Peanut Science 16, no. 2 (July 1, 1989): 63–66. http://dx.doi.org/10.3146/i0095-3679-16-2-1.
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Abstract Monoclonal antibodies (MABs) to peanut stripe virus (PStV) were obtained by fusing spleen cells from mice immunized with PStV to a mouse myeloma cell line. Two IgG2a subclass MABs, each binding with different antigenic sites, and rabbit polyclonal antibodies (PABs) were compared for their reaction to PStV and other serologically related plant viruses. One MAB reacted to PStV but not to other serologically related viruses. The other MAB reaction of the MABs and PABs to PStV in peanut leaf and seed tissues were compared by enzyme-linked immunosorbent assay (ELISA), dot-immunobinding assay, and Western blotting. MABs were found to be equivalent to or better than PABs in detecting PStV by ELISA and dot-immunobinding assay, but one MAB was not suitable for Western blotting.
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Uzair, Bushra, Rehana Kausar, Syeda Asma Bano, Sammer Fatima, Malik Badshah, Ume Habiba, and Fehmida Fasim. "Isolation and Molecular Characterization of a Model Antagonistic Pseudomonas aeruginosa Divulging In Vitro Plant Growth Promoting Characteristics." BioMed Research International 2018 (2018): 1–7. http://dx.doi.org/10.1155/2018/6147380.
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The use of microbial technologies in agriculture is currently expanding quite rapidly with the identification of new bacterial strains, which are more effective in promoting plant growth. In the present study 18 strains of Pseudomonas were isolated from soil sample of Balochistan coastline. Among isolated Pseudomonas strains four designated as SP19, SP22, PS24, and SP25 exhibited biocontrol activities against phytopathogenic fungi, that is, Rhizopus microsporus, Fusarium oxysporum, Aspergillus niger, Alternaria alternata, and Penicillium digitatum; PS24 identified as Pseudomonas aeruginosa by 16srRNA gene bank accession number EU081518 was selected on the basis of its antifungal activity to explore its potential as plant growth promotion. PS24 showed multiple plant growth promoting attributes such as phosphate solubilization activity, indole acetic acid (IAA), siderophore, and HCN production. In order to determine the basis for antifungal properties, antibiotics were extracted from King B broth of PS24 and analyzed by TLC. Pyrrolnitrin antibiotic was detected in the culture of strain PS24. PS24 exhibited antifungal activities found to be positive for hydrogen cyanide synthase Hcn BC gene. Sequencing of gene of Hcn BC gene of strain PS24 revealed 99% homology with the Pseudomonas aeruginosa strain PA01. The sequence of PS24 had been submitted in gene bank accession number KR605499. Ps. aeruginosa PS24 with its multifunctional biocontrol possessions can be used to bioprotect the crop plants from phytopathogens.
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Brooks, James D., Elissa C. Brown, Matthew R. Cooperberg, Lisa F. Newcomb, Peter Carroll, Ziding Feng, Martin Edwin Gleave, et al. "Association of declining PSA values with a lower risk of progression in the Canary Prostate Cancer Active Surveillance Study (PASS)." Journal of Clinical Oncology 31, no. 6_suppl (February 20, 2013): 88. http://dx.doi.org/10.1200/jco.2013.31.6_suppl.88.
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88 Background: Active Surveillance of men with low risk prostate cancer entails uncertainty for the patient and physician in determining risk of progression. While PSA determinations are frequently measured in men on active surveillance, no study thus far has found PSA velocity (PSAV) or PSA doubling times that identify patients at risk for clinical progression. However, based on observations in PASS, we hypothesized that men with negative PSAV might be at decreased risk for progression. Methods: From 723 PASS participants, we identified 396 who had at least 5 PSA values over 12-24 months after their diagnosis and prior to progression or last follow-up. Of the 396 patients, 56 progressed as defined by increase in Gleason score or increase in tumor involvement of the core biopsies to ≥ 34%, or increase in clinical stage while 340 men had no progression. PSAV is the slope of the log(PSA) values over time. ROC analysis was used with PSAV as the predictor of biopsy/clinical progression. Results: PSAV was mildly associated with clinical/biopsy progression with an AUC of 0.62 (95% CI: 0.54-0.70). Interestingly, by thresholding PSAV at 0, 150 of 396 men had negative a PSAV. Subjects with with a negative PSAV had a much lower rate of progression while on active surveillance. PSAV<0 had a negative predictive value for progression of 0.93 (95% CI: 0.88-0.97). The progression rate for those with negative PSA velocity was 11/150 ≈ 0.07, or 7% and 18% (45/246) for those with positive PSA velocity. Viewed another way, men with PSAV<0 had a 0.4 fold relative risk of progression compared to men with a positive PSAV or were 60% less likely to have clinical/grade progression than those with positive PSA velocity (95% CI: 0.21-0.75). Conclusions: Declining PSAV in men on active surveillance for clinically localized prostate cancer is associated with a lower risk of clinical progression. Clinical trial information: NCT00756665.
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Jiang, Nan, and D. M. Schrader. "Positronic Water,Ps2O." Physical Review Letters 81, no. 23 (December 7, 1998): 5113–16. http://dx.doi.org/10.1103/physrevlett.81.5113.
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Prasada Rao, R. D. V. J., G. Pio-Ribeiro, R. Pittman, D. V. R. Reddy, and J. W. Demski. "In Vitro Culture to Eliminate Peanut Stripe Virus from Peanut Seed." Peanut Science 22, no. 1 (January 1, 1995): 54–56. http://dx.doi.org/10.3146/pnut.22.1.0011.
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Abstract When excised seed axes of peanut seed, which tested serological positive for peanut stripe virus (PStV), were grown in vitro, 37.5% of the developing plants were PStV-negative. When plants were regenerated from shoot-tips (1 cm long) taken from in vitro-grown, virus-infected plants, 29.4% of them were virus-negative. PStV-positive plants obtained from virus-infected seed were grown for 12 wk on Murashige and Skoog (MS) medium supplemented with 40 mg/L ribavirin (MSR) resulted in 78.6% which tested negative for PStV. All plants from seed which tested positive for PStV were 100% identified as negative if grown on MSR for 16 wk. Similarly all plants were PStV negative when obtained from 1 cm long infected shoot tips (from plants grown on MS or MSR medium) if subsequently grown for 12 wk on MSR medium.
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Lee, Kai-Yi, Ying-Chieh Tsai, Sheng-Yao Wang, Yen-Po Chen, and Ming-Ju Chen. "Coculture Strategy for Developing Lactobacillus paracasei PS23 Fermented Milk with Anti-Colitis Effect." Foods 10, no. 10 (September 30, 2021): 2337. http://dx.doi.org/10.3390/foods10102337.
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Few studies have documented the effects of fermented milk on intestinal colitis, which are mediated by regulating various microbial and inflammatory processes. Here, we investigated the effects of fermented milk with Lactobacillus paracasei PS23 on intestinal epithelial cells in vitro and dextran sulfate sodium (DSS)-induced colitis in vivo. As L. paracasei PS23 grew poorly in milk, a coculture strategy with yogurt culture was provided to produce fermented milk (FM). The results indicated that the coculture exhibited a symbiotic effect, contributing to the better microbial and physicochemical property of the fermented milk products. We further evaluated the anti-colitis effect of fermented milk with L. paracasei PS23 in vitro. Both PS23-fermented milk (PS23 FM) and its heat-killed counterpart (HK PS23 FM) could protect or reverse the increased epithelial permeability by strengthening the epithelial barrier function in vitro by increasing transepithelial electrical resistance (TEER). In vivo analysis of the regulation of intestinal physiology demonstrated that low-dose L. paracasei PS23-fermented ameliorated DSS-induced colitis, with a significant attenuation of the bleeding score and reduction of fecal calprotectin levels. This anti-colitis effect may be exerted by deactivating the inflammatory cascade and strengthening the tight junction through the modification of specific cecal bacteria and upregulation of short-chain fatty acids. Our findings can clarify the role of L. paracasei PS23 in FM products when cocultured with yogurt culture and can elucidate the mechanisms of the anti-colitis effect of L. paracasei PS23 FM, which may be considered for therapeutic intervention.
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Li, Zhaolei, Zhihao Jiang, Xinxin Yang, Ning Yue, Xueting Wang, Kun Zhang, Andrew O. Jackson, Dawei Li, and Yongliang Zhang. "Construction of an Infectious Poa semilatent virus cDNA Clone and Comparisons of Hordeivirus Cytopathology and Pathogenicity." Phytopathology® 110, no. 1 (January 2020): 215–27. http://dx.doi.org/10.1094/phyto-06-19-0221-fi.
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Poa semilatent virus (PSLV), Lychnis ringspot virus (LRSV), and Barley stripe mosaic virus (BSMV) are members of the genus Hordeivirus in the family Virgaviridae. However, the biological properties and molecular genetics of PSLV have not been compared with other hordeiviruses. Here, we have constructed an infectious cDNA clone of the PSLV Canadian strain and provided evidence that PSLV differs from BSMV and LRSV. First, unlike the other two hordeiviruses that replicate in chloroplasts, PSLV induces dramatic structural changes in peroxisome during its infection in barley. The αa replication protein also localizes to peroxisomes, suggesting that PSLV replication occurs in peroxisomes. Second, PSLV encodes a γb protein that shares 19 to 23% identity with those of other hordeiviruses, and its activity as a viral suppressor of RNA (VSR) silencing is distinct from those of BSMV and LRSV. Substitution of the BSMV γb protein with that of PSLV or LRSV revealed a negative correlation between VSR activity and symptom severity of the recombinant BSMV derivatives. Intriguingly, the Ser-Lys-Leu (SKL) peroxisome-targeting signals differ among γb proteins of various hordeiviruses, including some BSMV strains. The presence of the C-terminal SKL motif in the γb protein impairs its silencing suppressor activity and influences symptoms. Finally, we developed a PSLV-based virus-induced gene silencing vector that induced strong and effective silencing phenotypes of endogenous genes in barley, wheat, and millet. Our results shed new light on hordeivirus pathogenesis and evolution, and provide an alternative tool for genomics studies of model hosts and economically important monocots.
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Hapsoro, Dwi, Hajrial Aswidinnoor, Rusmilah Suseno, Jumanto Jumanto, and Sudarsono Sudarsono. "TRANSGENE IDENTITY AND NUMBER OF INTEGRATION SITES AND THEIR CORRELATION WITH RESISTANCE TO PStV IN TRANSGENIC PEANUTS CARRYING PEANUT STRIPE VIRUS (PStV) COAT PROTEIN GENE." Jurnal Hama dan Penyakit Tumbuhan Tropika 7, no. 1 (March 4, 2007): 39–47. http://dx.doi.org/10.23960/j.hptt.1739-47.
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Transgene Identity and Number of Integration Sites and Their Correlation with Resistance To PStV in Transgenic Peanuts Carrying Peanut Stripe Virus (PStV) Coat Protein Gene. This research aimed to determine (1) the identity and copy number of PStV cp gene in transgenic peanut plants carrying PStV cp gene and (2) correlation between the identity and the number of integration sites and resistance to PStV infection. One T0 transgenic peanut was selfed up to five generations. T2, T3, and T5 plants were mechanically inoculated with PStV. Samples of T5 plants derived from several different T4 plants were subjected to Southern analysis to confirm the integration of PStV cp gene and to determine its identity and copy number. The Southern analysis showed three bands of different size, i.e. 1.1 kb, 1.3 kb, and 5.8 kb. Most of the lines of T5 generation have one insertion site, suggesting that the three insertion sites were located in different loci. Based on the phenotypic data, the transgenes of 1.1 kb and 1.3 kb were functional, resulting in resistant or recovery phenotype, while that of 5.8 kb was not functional. Copy number apparently had no effects on the phenotypes.
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Ressler, Steven J., and David R. Rowley. "The WFDC1 gene: role in wound response and tissue homoeostasis." Biochemical Society Transactions 39, no. 5 (September 21, 2011): 1455–59. http://dx.doi.org/10.1042/bst0391455.
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The present evaluates the key features of the WFDC1 [WAP (whey acidic protein) four disulfide core 1] gene that encodes ps20 (20 kDa prostate stromal protein), a member of the WAP family. ps20 was first characterized as a growth inhibitory activity that was secreted by fetal urogenital sinus mesenchymal cells. Purified ps20 exhibited several activities that centre on cell adhesion, migration and proliferation. The WFDC1 gene was cloned, contained seven exons, and was mapped to chromosome 16q24, suggesting that it may function as a tumour suppressor; however, direct evidence of this has not emerged. In vivo, ps20 stimulated angiogenesis, although expression of WFDC1/ps20 was down-regulated in the reactive stroma tumour microenvironment in prostate cancer. WFDC1 expression is differential in other cancers and inflammatory conditions. Recent studies point to a role in viral infectivity. Although mechanisms of action are not fully understood, WFDC1/ps20 is emerging as a secreted matricellular protein that probably affects response to micro-organisms and tissue repair homoeostasis.
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Ciatto, S., R. Bonardi, C. Lombardi, M. Zappa, G. Gervasi, and G. Cappelli. "Analysis of PSA Velocity in 1666 Healthy Subjects Undergoing Total PSA Determination at Two Consecutive Screening Rounds." International Journal of Biological Markers 17, no. 2 (April 2002): 79–83. http://dx.doi.org/10.1177/172460080201700201.
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The study purpose was to assess PSA velocity (PSAV) in healthy subjects in order to establish a reliable cutoff for the differential diagnosis of prostate cancer in a screening setting. We studied a series of 1666 healthy men aged 55 to 74 years undergoing two total PSA determinations at a four-year interval within a population-based randomized screening trial at the Centro per lo Studio e la Prevenzione Oncologica of Florence. First and second screening round PSA assays (PSA1 and PSA2) were carried out with the same method and by the same laboratory. PSAV (PSA1–PSA2/year) was determined in non-cancer subjects in the overall series or in specific age and PSA subgroups, and in subjects with cancer detected at the second screening round. Average PSAV in 1648 non-cancer subjects was 0.07 ng/mL/year (range −2.18+5.99, 95% CI 0.05–0.09); at least one third of subjects showed a decrease in PSA (negative PSAV), mostly of limited magnitude and in the low PSA range. Average PSAV in the 18 cancer patients was 1.16 ng/mL/year (range 0.10–5.6, 95% CI 0.56–1.77), which is significantly higher (p<0.01) than in non-cancer subjects. None of the cancer patients showed a PSA decrease over time. Whatever cutoff was taken for PSAV, its power to discriminate cancer was limited: in particular the previously used PSAV cutoff of 0.75 ng/mL/year would have included only 42 of the 1648 non-cancer subjects (specificity 97.5%) but excluded eight of the 18 cancer patients (sensitivity 55.5%). At best, with the adopted screening protocol PSAV (cutoff 0.10 ng/mL/year) could have spared 27.9% of non-cancer subjects with PSA ≥2.5 ng/mL further diagnostic assessment and 22.7% of non-cancer subjects with PSA ≥4 ng/mL random sextant biopsy, while missing no cancers. This study provides a reliable estimate of PSAV based on a large unbiased population sample. PSAV is widely variable over time, particularly at low PSA values. PSAV might be of value as an indicator for diagnostic assessment and random sextant biopsy in a screening setting.
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Lee, Mon-Chien, Chin-Shan Ho, Yi-Ju Hsu, and Chi-Chang Huang. "Live and Heat-Killed Probiotic Lactobacillus paracasei PS23 Accelerated the Improvement and Recovery of Strength and Damage Biomarkers after Exercise-Induced Muscle Damage." Nutrients 14, no. 21 (October 30, 2022): 4563. http://dx.doi.org/10.3390/nu14214563.
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Excessive, high-intensity or inappropriate exercise may cause muscle damage. How to speed up recovery and reduce exercise discomfort are currently very important issues for athletes and sports people. Past research has shown that probiotics can improve inflammation and oxidative stress, as well as improve exercise performance and antifatigue. However, further research is needed to confirm the recovery benefits for muscle damage. In this double-blind design study, all subjects were randomly assigned to placebo, a live Lactobacillus paracasei group (L-PS23, 2 × 1010 colony forming unit (CFU)/day), or a heat-killed L. paracasei group (HK-PS23, 2 × 1010 cells/day), and supplemented for six consecutive weeks. Afterwards, subjects completed 100 maximal vertical jumps to bring about exercise-induced muscle damage (EIMD). Countermovement jump (CMJ), isometric mid-thigh pull (IMTP), and Wingate anaerobic test (WAnT), as well as blood tests for markers of muscle damage and inflammation were made pre-exercise and 3, 24, 48 h post exercise. The results show that both L-PS23 and HK-PS23 supplementation significantly slowed the loss of muscle strength after muscle injury, and they significantly reduced the production of markers of muscle damage and inflammation (p < 0.05). In addition, L-PS23 and HK-PS23 had the benefits of accelerating the recovery and improvement of muscle strength, the blood markers of muscle injury and inflammation, and slowing the decline in testosterone concentrations (p < 0.05). Especially in the HK-PS23 supplemented group, there was a better trend. In conclusion, we found that L-PS23 or HK-PS23 supplementation for six weeks prevented strength loss after muscle damage and improved blood muscle damage and inflammatory markers, with protective, accelerated recovery and anti-fatigue benefits.
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Tabler, Martin, Martina Schnölzer, and Heinz L. Sänger. "Molecular cloning of potato spindle tuber viroid (PSTV) cDNA synthesized by enzymatic elongation of PSTV-specific DNA primers: A general strategy for viroid cloning." Bioscience Reports 5, no. 2 (February 1, 1985): 143–58. http://dx.doi.org/10.1007/bf01117061.
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Different cDNAs were synthesized by primer extension from the RNA of the severe strain KF 440 of potato spindle tuber viroid (PSTV) with the aid of reverse transcriptase using three PSTV-specific DNA molecules as primers. The cDNAs were made double-stranded and cloned into plasmid pBR 322. Various overlapping subgenomic DNA fragments were prepared from these clones and recombined in two different ways. In both cases a PSTV DNA copy was obtained which represented the entire PSTV RNA genome. The sequence of the DNA of one of the resulting full-length clones was identical with the original PSTV isolate, whereas the other clone showed one nucleotide change. On the basis of these results the advantages and problems of different strategies for the molecular cloning of the circular viroid RNA genome are discussed.
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Ross, L. F., R. E. Lynch, E. J. Conkerton, J. W. Demski, D. J. Daigle, and C. McCombs. "The Effect of Peanut Stripe Virus Infection on Peanut Composition." Peanut Science 16, no. 1 (January 1, 1989): 43–45. http://dx.doi.org/10.3146/i0095-3679-16-1-9.
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Abstract Peanuts (Arachis hypogaea L.), cultivar Florunner, from plants inoculated with peanut stripe virus (PStV) were evaluated for chemical composition in comparison with peanuts from uninoculated plants. At harvest, seed were collected from plants which had been mechanically inoculated with PStV at emergence, or 20, 40, or 60 days after emergence and from uninoculated plants. The seed from PStV-infected plants had increases in manganese, selenium, zinc, iron, tartaric acid, raffinose, glucose, fructose, and total carbohydrate contents as compared to seed from uninoculated plants. Sucrose was increased in seed from plants inoculated with PStV at time of emergence. There was a decrease in the concentration of potassium, magnesium, protein, and total soluble phenolics of seed from plants inoculated with PStV. There were no changes in the concentration of stachyose, inositol, phosphorus, sulfur, calcium, copper, and oil.
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Shao, Yichuan, Xingjia Yao, Liwei Tian, and Hanning Chen. "A Multiswarm Optimizer for Distributed Decision Making in Virtual Enterprise Risk Management." Discrete Dynamics in Nature and Society 2012 (2012): 1–24. http://dx.doi.org/10.1155/2012/904815.
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We develop an optimization model for risk management in a virtual enterprise environment based on a novel multiswarm particle swarm optimizer called PS2O. The main idea of PS2O is to extend the single population PSO to the interacting multiswarms model by constructing hierarchical interaction topology and enhanced dynamical update equations. With the hierarchical interaction topology, a suitable diversity in the whole population can be maintained. At the same time, the enhanced dynamical update rule significantly speeds up the multiswarm to converge to the global optimum. With five mathematical benchmark functions, PS2O is proved to have considerable potential for solving complex optimization problems. PS2O is then applied to risk management in a virtual enterprise environment. Simulation results demonstrate that the PS2O algorithm is more feasible and efficient than the PSO algorithm in solving this real-world problem.
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Zeyong, Xu, Colleen M. Higgins, Chen Kunrong, Ralf G. Dietzgen, Zhang Zhongyi, Yang Liying, and Fang Xiaoping. "Evidence for a Third Taxonomic Subgroup of Peanut Stunt Virus from China." Plant Disease 82, no. 9 (September 1998): 992–98. http://dx.doi.org/10.1094/pdis.1998.82.9.992.
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On the basis of host reactions and serology, six Chinese peanut stunt virus (PSV) strains were found to be distinct from PSV-E and PSV-W, two type strains representing distinct serological subgroups. Chinese PSV strains were characterized by infecting Chenopodium amaranticolor and C. quinoa systemically. All Chinese strains were serologically closely related to each other, but distinct from PSV-E and more distant from PSV-W. Using two PSV-specific primers designed from conserved regions of the PSV RNA3 nucleotide sequence, cDNA transcribed from RNA3 of two Chinese PSV strains, Mi and S, was amplified by PCR and cloned. The sequenced cDNA of the two PSV strains included 654 nt of the coat protein (CP) gene. The identity of the CP gene nucleotide sequence between PSV-Mi and PSV-S was 99.0%, with 99.5% amino acid identity. Identity of the CP gene nucleotide sequence was 75.6 to 77.8% between PSV-Mi and -S (the two Chinese strains) and PSV-ER and -J in PSV subgroup I; and 74.1 to 74.4% between PSV-Mi and -S, and PSV-W in subgroup II. Based on these results, we propose placing PSV Chinese strains into a new PSV subgroup III.
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Thureen, Dean R., and Calvin L. Keeler. "Psittacid Herpesvirus 1 and Infectious Laryngotracheitis Virus: Comparative Genome Sequence Analysis of Two Avian Alphaherpesviruses." Journal of Virology 80, no. 16 (August 15, 2006): 7863–72. http://dx.doi.org/10.1128/jvi.00134-06.
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ABSTRACT Psittacid herpesvirus 1 (PsHV-1) is the causative agent of Pacheco's disease, an acute, highly contagious, and potentially lethal respiratory herpesvirus infection in psittacine birds, while infectious laryngotracheitis virus (ILTV) is a highly contagious and economically significant avian herpesvirus which is responsible for an acute respiratory disease limited to galliform birds. The complete genome sequence of PsHV-1 has been determined and compared to the ILTV sequence, assembled from published data. The PsHV-1 and ILTV genomes exhibit similar structural characteristics and are 163,025 bp and 148,665 bp in length, respectively. The PsHV-1 genome contains 73 predicted open reading frames (ORFs), while the ILTV genome contains 77 predicted ORFs. Both genomes contain an inversion in the unique long region similar to that observed in pseudorabies virus. PsHV-1 is closely related to ILTV, and it is proposed that it be assigned to the Iltovirus genus. These two avian herpesviruses represent a phylogenetically unique clade of alphaherpesviruses that are distinct from the Marek's disease-like viruses (Mardivirus). The determination of the complete genomic nucleotide sequences of PsHV-1 and ILTV provides a tool for further comparative and functional analysis of this unique class of avian alphaherpesviruses.
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Flores-Fraile, María-Carmen, Bárbara Yolanda Padilla-Fernández, Sebastián Valverde-Martínez, Magaly Marquez-Sanchez, María-Begoña García-Cenador, María-Fernanda Lorenzo-Gómez, and Javier Flores-Fraile. "The Association between Prostate-Specific Antigen Velocity (PSAV), Value and Acceleration, and of the Free PSA/Total PSA Index or Ratio, with Prostate Conditions." Journal of Clinical Medicine 9, no. 11 (October 23, 2020): 3400. http://dx.doi.org/10.3390/jcm9113400.
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Introduction: Prostate-specific antigen velocity (PSAV) is used to monitor men with clinical suspicion of prostate cancer (PCa), with a normal cut-off point of 0.3–0.5 ng/mL/year. The aim of the study is to establish the predictive capacity of PSAV (value and acceleration) and of the free PSA/total PSA index or ratio. Method: Prospective multicentre observational study in 2035 men of over 47 years of age. Inclusion criteria: men who wished to be informed on the health of their prostate. Exclusion criteria: men with a previously diagnosed prostate condition. Groups: GA: (n = 518): men with serum PSA equal to or greater than 2.01 ng/mL. GB: (n = 775): men with serum PSA greater than or equal to 0.78 ng/mL and less than 2.01 ng/mL. GC: (n = 742): men with serum PSA less than 0.78 ng/mL. Variables: prostate-specific antigen (PSA); age; body mass index (BMI); PSA velocity (PSAV) (ng/mL per year); free PSA/total PSA index (iPSA); PSAV acceleration (increasing: positive, or decreasing: negative); prostate diagnosis (benign prostatic hyperplasia (BPH), prostatic intraepithelial neoplasia (PIN), or infectious and non-infectious prostatitis and prostatic adenocarcinoma (PCa)); de novo diagnoses of urinary tract diseases or conditions; concomitant treatments, diseases and conditions; final diagnosis of prostate health. Results: Mean age 62.35 years (SD 8.12), median 61 (47–94); age was lowest in GC. Mean BMI was 27.89 kg/m2 (SD 3.96), median 27.58 (18.56–57.13); no differences between groups. Mean PSAV was 0.69, SD 2.16, median 0.13 (0.001–34.46); PSAV was lowest in GC. Mean iPSA was 27.39 u/L (SD 14.25), median 24.29 (3.7–115); iPSA was lowest in GA. PSAV had more positive acceleration in GA and more negative acceleration in GC. There were 1600 (78.62%) cases of normal prostate or BPH, 322 (15.82%) cases of PIN or non-infectious prostatitis, and 113 (5.55%) cases of PCa. There were more cases of BPH in GC and more cases of PIN or prostatitis and cancer in GA (p = 0.00001). De novo diagnoses: 15 cases of urinary incontinence (UI), 16 discomfort/pain in LUT, 112 cases of voiding disorders, 12 urethral strictures, 19 hematuria, 51 cystitis, 3 pyelonephritis, 4 pelvic inflammatory disease; no differences were found between groups. In the multivariate analysis, PSAV and the direction of PSAV acceleration (positive or negative) were the variables which were correlated most strongly with prostate health. iPSA was associated with the presence of prostatitis, PCa, and BPH. Men in GA had more prostatitis, PCa, treatment with alpha blockers, and history of previous smoking. GB had more cases of BPH and more positive acceleration of PSAV. GC had more normal prostates, more BPH, more use of ranitidine, and more PSAV with negative acceleration. Conclusions: PSAV, direction of PSAV acceleration, and iPSA in PSA cut-off points of 0.78 ng/mL and 2.01 ng/mL in a priori healthy men over 47 predict the probability of benign or malignant pathology of the prostate.
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Froud, K. J., N. Cogger, and R. M. Beresford. "Relationships between kiwifruit bacterial canker disease and kiwifruit productivity." New Zealand Plant Protection 67 (January 8, 2014): 34–40. http://dx.doi.org/10.30843/nzpp.2014.67.5719.
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Bacterial canker disease caused by a virulent strain of Pseudomonas syringae pv actinidiae (PsaV) has affected kiwifruit vines in New Zealand since 2010 This study investigated the association of PsaV with productivity within Hayward and Hort16A varieties PsaV infection status and date of diagnosis for 3309 infected orchards were provided by Kiwifruit Vine Health while Zespri provided productivity data Linear regression models were constructed to determine the relationship between production and PsaV infection in Hayward and Hort16A orchards Results showed a significant relationship between the numbers of weeks PsaV was detected in Hort16A orchards and a reduction in productivity This was likely due to the removal of Hort16A vines or productive areas of canopy in response to the presence of severe symptoms within an orchard A similar significant relationship was also found in Hayward orchards although the reduction in productivity was smaller and took longer to develop than in Hort16A
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Van Bressem, Marie-Françoise, Patricia Cassonnet, Annabel Rector, Christian Desaintes, Koen Van Waerebeek, Joanna Alfaro-Shigueto, Marc Van Ranst, and Gérard Orth. "Genital warts in Burmeister's porpoises: characterization of Phocoena spinipinnis papillomavirus type 1 (PsPV-1) and evidence for a second, distantly related PsPV." Journal of General Virology 88, no. 7 (July 1, 2007): 1928–33. http://dx.doi.org/10.1099/vir.0.82694-0.
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We identified sequences from two distantly related papillomaviruses in genital warts from two Burmeister's porpoises, including a PV antigen-positive specimen, and characterized Phocoena spinipinnis papillomavirus type 1 (PsPV-1). The PsPV-1 genome comprises 7879 nt and presents unusual features. It lacks an E7, an E8 and a bona fide E5 open reading frame (ORF) and has a large E6 ORF. PsPV-1 L1 ORF showed the highest percentage of nucleotide identity (54–55 %) with human papillomavirus type 5, bovine papillomavirus type 3 (BPV-3) and Tursiops truncatus papillomavirus type 2 (TtPV-2). This warrants the classification of PsPV-1 as the prototype of the genus Omikronpapillomavirus. PsPV-1 clustered with TtPV-2 in the E6 and E1E2 phylogenetic trees and with TtPV-2 and BPV-3 in the L2L1 tree. This supports the hypothesis that PV evolution may not be monophyletic across all genes.
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Casquero León, Jorge Luis, Giancarlo Andreé Valle González, Juan Carlos Ávila Alegría, José Raúl Paredes Salas, and Luis Arturo Pedro Saona Ugarte. "Relación entre la pérdida sanguíneaestimada y la pérdida sanguínea calculada en partos por cesárea en nulíparas." Revista Peruana de Ginecología y Obstetricia 58, no. 2 (February 16, 2014): 115–21. http://dx.doi.org/10.31403/rpgo.v58i83.
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Objetivo: Correlacionar la pérdida sanguínea estimada visualmente (PSev) con la pérdida sanguínea calculada (PSc) que se produce durante el parto por cesárea en pacientes nulíparas. Diseño: Estudio descriptivo de correlación. Institución: Hospital Nacional Cayetano Heredia, Lima, Perú. Participantes: Gestantes nulíparas con parto por cesárea. Intervenciones: Se revisó 160 historias clínicas de pacientes nulíparas atendidas de parto por cesárea a término, en el año 2011. Los datos obtenidos fueron analizados estadísticamente según el programa STATA V10,1 para Windows. Principales medidas de resultados: Pérdida sanguínea. Resultados: Las medianas de la PSev y de la PSc fueron 500 mL (500 a 600 mL) y 421,4 mL (319,85 a 559,65 mL), siendo estadísticamente diferentes, con p < 0,001. Para PSc < 500 mL, la mediana fue 351,95 mL (283,60 a 419,20 mL) y la mediana de PSev 500mL (500 a 600 mL), siendo estadísticamente diferente, con p < 0,001. Para PSc >1 000 mL, la mediana fue 1 207,80 mL (1 039,60 a 1 419,00 mL) y la mediana de PSev 500mL (500 a 600 mL), siendo estadísticamente diferente, con p < 0,001. La mediana de la caída del hematocrito fue 3% (3 a 4%). El coeficiente de correlación de Spearman para PSev y PSc fue 0,302 (p < 0,001). Conclusiones: Se halló correlación débil entre la PSev y la PSc, estadísticamente significativa. Para sangrado intraparto menor a 500 mL, la PSev fue sobreestimada, y para sangrado intraparto mayor a 1 000mL (hemorragia posparto), PSev fue subestimada. La estimación visual del sangrado intraparto es un método inexacto para calcular volúmenes sanguíneos, por lo cual se requiere métodos alternativos efectivos, que incluyen el uso de una fórmula que utiliza la estimación del volumen sanguíneo materno y la variación del hematocrito.
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Pappu, S. S., H. R. Pappu, C. A. Chang, A. K. Culbreath, and J. W. Todd. "Differentiation of Biologically Distinct Peanut Stripe Potyvirus Strains by a Nucleotide Polymorphism-Based Assay." Plant Disease 82, no. 10 (October 1998): 1121–25. http://dx.doi.org/10.1094/pdis.1998.82.10.1121.
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A necrotic strain of peanut stripe potyvirus (PStV-Ts) was used to design and test strain-differentiating oligonucleotides. The 3′ region of PStV-Ts, including a part of the NIb region, the complete coat protein (CP) gene, and the 3′-untranslated region, was cloned and sequenced. PStV-Ts had a high degree of sequence identity (92 to 95%) to the known non-necrotic (blotch) strains both at the nucleotide and amino acid sequence levels. Nucleotide sequence differences unique to the necrotic strain were identified when compared to the available non-necrotic isolates of PStV. Nucleotide polymorphism in the CP gene sequences was utilized in designing oligonucleotides that were specific to the necrotic strain, and were employed in an assay to differentiate the necrotic strain from non-necrotic. The 3′ end mismatch in the oligonucleotides contributed in particular to the differentiation of the strains. This approach facilitated rapid, sensitive, and reliable detection and differentiation of PStV strains.
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SINGH, R. P., T.-L. DeHAAN, and A. S. JASWAL. "A SURVEY OF THE INCIDENCE OF POTATO SPINDLE TUBER VIROID IN PRINCE EDWARD ISLAND USING TWO TESTING METHODS." Canadian Journal of Plant Science 68, no. 4 (October 1, 1988): 1229–36. http://dx.doi.org/10.4141/cjps88-152.
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Return-polyacrylamide gel electrophoresis (R-PAGE) and nucleic acid hybridization (dot-blot) were used for the detection of potato spindle tuber viroid (PSTV) from potato leaves. Both methods detected potato plants experimentally infected in the first season or those produced from infected tubers (secondarily infected). PSTV concentration was lower in the first-season infected plants than those in the second. Both methods detected PSTV in a single leaf disc from field-grown infected plants combined with 399 – 499 discs from field-grown healthy plants. The sensitivity of detection by R-PAGE was lower for certain cultivars and increased with the age of plants. About 85 000 leaf samples collected from 123 tablestock fields, 170 seed fields, and 63 cultivars from the Fox Island Elite Seed Farm in Prince Edward Island were found to be free from PSTV infection. Reasons for PSTV absence in the potato crop are discussed.Key words: Diagnosis, dot-blot, return polyacrylamide gel electrophoresis, nucleic acid hybridization, Solanum tuberosum, potato
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Lynch, R. E., J. W. Demski, W. D. Branch, C. C. Holbrook, and L. W. Morgan. "Influence of Peanut Stripe Virus on Growth, Yield, and Quality of Florunner Peanut1." Peanut Science 15, no. 2 (July 1, 1988): 47–52. http://dx.doi.org/10.3146/i0095-3679-15-2-2.
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Abstract The influence of peanut stripe virus (PStV) on growth, yield, and grade of Florunner peanut and percent seed transmissions was determined under field conditions during 1985 and 1986. Plants were artificially inoculated with PStV and infection was confirmed by enzyme-linked immunosorbent assays. Under the conditions of these tests, PStV did not significantly influence growth, yield, or grade of Florunner peanut, and seed infection averaged less than 2 percent.
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Gu, Z., X. Wang, J. Yu, F. Shao, and Y. Zhang. "PSIV-1 microRNA-122." Journal of Animal Science 96, suppl_3 (December 2018): 128. http://dx.doi.org/10.1093/jas/sky404.283.
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Ze-Yong, Xu, Yan Li-Ying, Chen Kun-Rong, and Marcel Prins. "RNA3 nucleotide sequence analyses ofpeanut stunt virusMi strain." Chinese Journal of Agricultural Biotechnology 2, no. 1 (April 2005): 33–38. http://dx.doi.org/10.1079/cjb200552.
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AbstractNucleotide sequence of full-length cDNA ofpeanut stunt virus(PSV) Mi strain RNA3 was determined and compared with those of PSV-ER and -J (subgroup I) and PSV-W (subgroup II), strains ofcucumber mosaic virus(CMV) andtomato aspermy virus(TAV). PSV-Mi RNA3 consists of 2170 nt and has two open reading frames, encoding a putative movement protein (3a protein) and a coat protein (CP). PSV-Mi RNA3 is 77.7% and 78.5% identical to those of PSV-ER and -J, whereas it shares 76.6% identity with PSV-W. Nucleotide identity of3aandcpgenes between PSV strains Mi and ER, J and W was 78.3–79.3% and 74.4–77.8%, respectively. Amino acid identity of 3a and CP between PSV-Mi and -ER, -J and -W was 73.9–77.4% and 64.8–77.5%, respectively. RNA3 of PSV-Mi (GenBank accession no. AY775057) had a varied intercistronic and 5′-untranslated region compared with those of PSV strains ER, J and W. Results indicate that PSV-Mi represents a new PSV subgroup from China, designated as subgroup III.
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Liao, J. F., C. C. Hsu, G. T. Chou, J. S. Hsu, M. T. Liong, and Y. C. Tsai. "Lactobacillus paracasei PS23 reduced early-life stress abnormalities in maternal separation mouse model." Beneficial Microbes 10, no. 4 (April 19, 2019): 425–36. http://dx.doi.org/10.3920/bm2018.0077.
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Maternal separation (MS) has been developed as a model for inducing stress and depression in studies using rodents. The concept of the gut-brain axis suggests that gut health is essential for brain health. Here, we present the effects of administration of a probiotic, Lactobacillus paracasei PS23 (PS23), to MS mice against psychological traits including anxiety and depression. The administration of live and heat-killed PS23 cells showed positive behavioural effects on MS animals, where exploratory tendencies and mobility were increased in behavioural tests, indicating reduced anxiety and depression compared to the negative control mice (P<0.05). Mice administered with both live and heat-killed PS23 cells also showed lower serum corticosterone levels accompanied by higher serum anti-inflammatory interleukin 10 (IL-10) levels, compared to MS separated mice (P<0.05), indicating a stress-elicited response affiliated with increased immunomodulatory properties. Assessment of neurotransmitters in the brain hippocampal region revealed that PS23 affected the concentrations of dopaminergic metabolites differently than the control, suggesting that PS23 may have improved MS-induced stress levels via neurotransmitter pathways, such as dopamine or other mechanisms not addressed in the current study. Our study illustrates the potential of a probiotic in reversing abnormalities induced by early life stress and could be an alternative for brain health along the gut-brain axis.
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Chu, Chishih, Cheng-Hsun Chiu, Wan-Yu Wu, Chi-Hong Chu, Tsui-Ping Liu, and Jonathan T. Ou. "Large Drug Resistance Virulence Plasmids of Clinical Isolates of Salmonella enterica Serovar Choleraesuis." Antimicrobial Agents and Chemotherapy 45, no. 8 (August 1, 2001): 2299–303. http://dx.doi.org/10.1128/aac.45.8.2299-2303.2001.
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ABSTRACT Salmonella enterica serovar Choleraesuis generally causes systemic human salmonellosis without diarrhea, and therefore, antimicrobial treatment is essential for such patients. The drug resistance information on this organism is thus of high value. Serovar Choleraesuis usually harbors a virulence plasmid (pSCV) of 50 kb in size. Of the 16 clinical isolates identified to be serovar Choleraesuis, all except one harbored a pSCV and seven of them carried a pSCV of more than 125 kb in size. A pSCV was defined as a plasmid carrying spvC and characteristic deletions detected by PCR and by DNA-DNA hybridization (for the former criterion). The results of PCR, restriction fragment profiles, and Southern DNA-DNA hybridizations of the profiles all indicated that such larger pSCVs were derived from the 50-kb plasmid recombined with non-pSCVs found in some clinical isolates. Fifteen of the 17 strains, including a laboratory strain, were then tested for drug resistance against 16 antibiotics with E-test and the dilution method. The laboratory strain, which harbored a 50-kb pSCV and a 6-kb non-pSCV, was resistant only to sulfonamides (SUL), and its resistance gene, sulII, checked with PCR and DNA-DNA hybridization, was located on the 6-kb non-pSCV. All 14 clinical strains were resistant to multiple drugs. Of the 14, 7 were resistant to SUL, and the resistance gene was located on a plasmid. ThesulII gene, but not bla TEM-1, was carried only on the 6-kb non-pSCV. Of the remaining six large plasmids, three of 90 kb, two of 136 kb, and one of 140 kb, the last three were pSCVs and carried the other SUL gene (sulI) and thebla TEM-1 gene. The six strains were also resistant to trimethoprim-sulfamethoxazole. None of the 50-kb pSCVs carried resistance genes. These drug resistance genes on the large pSCVs were apparently also acquired through recombination.
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Hu, C. C., and S. A. Ghabrial. "Molecular Evidence That Strain BV-15 of Peanut Stunt Cucumovirus Is a Reassortant Between Subgroup I and II Strains." Phytopathology® 88, no. 2 (February 1998): 92–97. http://dx.doi.org/10.1094/phyto.1998.88.2.92.
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In Northern hybridization assays, RNA1 of peanut stunt virus (PSV) strain BV-15 hybridized strongly with a cloned cDNA probe to RNA1 from strain PSV-W (subgroup II). Cloned probes to PSV-W RNA2 and RNA3, however, did not hybridize with the corresponding RNAs from strain BV-15. The complete nucleotide sequence of PSV-BV-15 RNA2 has been determined, and sequence comparison analysis showed that it is closely related to PSV subgroup I strains; the percent nucleotide sequence identity between PSV-BV-15 RNA2 and RNA2 sequences from PSV subgroup I and II strains were 90.5 and 75%, respectively. The possibility that PSV-BV-15 RNA2 may contain short regions derived from a subgroup II strain (i.e., represent a mosaic structure indicative of recombination) was investigated. Results indicated, however, that the entire PSV-BV-15 RNA2 sequence is derived from a subgroup I strain. PSV-BV-15 RNA3 has previously been shown to belong to subgroup I strains. These results thus establish that PSV strain BV-15 is a natural reassortant between PSV subgroups I and II strains. A reverse transcription-po-lymerase chain reaction assay is proposed for differentiating between this reassortant strain and PSV strains in subgroups I and II.
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Hajimorad, M. R., A. L. Eggenberger, and J. H. Hill. "Loss and Gain of Elicitor Function of Soybean Mosaic Virus G7 Provoking Rsv1-Mediated Lethal Systemic Hypersensitive Response Maps to P3." Journal of Virology 79, no. 2 (January 15, 2005): 1215–22. http://dx.doi.org/10.1128/jvi.79.2.1215-1222.2005.
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ABSTRACT Rsv1, a single dominant resistance gene in soybean PI 96983 (Rsv1), confers extreme resistance against all known American strains of Soybean mosaic virus (SMV), except G7 and G7d. SMV-G7 provokes a lethal systemic hypersensitive response (LSHR), whereas SMV-G7d, an experimentally evolved variant of SMV-G7, induces systemic mosaic. To identify the elicitor of Rsv1-mediated LSHR, chimeras were constructed by exchanging fragments between the molecularly cloned SMV-G7 (pSMV-G7) and SMV-G7d (pSMV-G7d), and their elicitor functions were assessed on PI 96983 (Rsv1). pSMV-G7-derived chimeras containing only P3 of SMV-G7d lost the elicitor function, while the reciprocal chimera of pSMV-G7d gained the function. The P3 regions of the two viruses differ by six nucleotides, of which two are translationally silent. The four amino acid differences are located at positions 823, 915, 953, and 1112 of the precursor polypeptide. Analyses of the site-directed point mutants of both the viruses revealed that nucleotide substitutions leading to translationally silent mutations as well as reciprocal amino acid substitution at position 915 did not influence the loss or gain of the elicitor function. pSMV-G7-derived mutants with amino acid substitutions at any of the other three positions lost the ability to provoke LSHR but induced SHR instead. Two concomitant amino acid substitutions at positions 823 (V to M) and 953 (K to E) abolished pSMV-G7 elicitor function, provoking Rsv1-mediated SHR. Conversely, pSMV-G7d gained the elicitor function of Rsv1-mediated LSHR by a single amino acid substitution at position 823 (M to V), and mutants with amino acid substitutions at position 953 or 1112 induced SHR instead of mosaic. Taken together, the data suggest that strain-specific P3 of SMV is the elicitor of Rsv1-mediated LSHR.
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Carvalho, Alysson R., Peter M. Spieth, Andreas Güldner, Maximilano Cuevas, Nadja C. Carvalho, Alessandro Beda, Stephanie Spieth, et al. "Distribution of regional lung aeration and perfusion during conventional and noisy pressure support ventilation in experimental lung injury." Journal of Applied Physiology 110, no. 4 (April 2011): 1083–92. http://dx.doi.org/10.1152/japplphysiol.00804.2010.
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In acute lung injury (ALI), pressure support ventilation (PSV) may improve oxygenation compared with pressure-controlled ventilation (PCV), and benefit from random variation of pressure support (noisy PSV). We investigated the effects of PCV, PSV, and noisy PSV on gas exchange as well as the distribution of lung aeration and perfusion in 12 pigs with ALI induced by saline lung lavage in supine position. After injury, animals were mechanically ventilated with PCV, PSV, and noisy PSV for 1 h/mode in random sequence. The driving pressure was set to a mean tidal volume of 6 ml/kg and positive end-expiratory pressure to 8 cmH2O in all modes. Functional variables were measured, and the distribution of lung aeration was determined by static and dynamic computed tomography (CT), whereas the distribution of pulmonary blood flow (PBF) was determined by intravenously administered fluorescent microspheres. PSV and noisy PSV improved oxygenation and reduced venous admixture compared with PCV. Mechanical ventilation with PSV and noisy PSV did not decrease nonaerated areas but led to a redistribution of PBF from dorsal to ventral lung regions and reduced tidal reaeration and hyperinflation compared with PCV. Noisy PSV further improved oxygenation and redistributed PBF from caudal to cranial lung regions compared with conventional PSV. We conclude that assisted ventilation with PSV and noisy PSV improves oxygenation compared with PCV through redistribution of PBF from dependent to nondependent zones without lung recruitment. Random variation of pressure support further redistributes PBF and improves oxygenation compared with conventional PSV.
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Chadwick, M. P., F. E. B. May, and B. R. Westley. "Production and comparison of mature single-domain ‘trefoil’ peptides pNR-2/pS2 Cys58 and pNR-2/pS2 Ser58." Biochemical Journal 308, no. 3 (June 15, 1995): 1001–7. http://dx.doi.org/10.1042/bj3081001.
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The preparation and purification of recombinant mature pNR-2/pS2, a single-domain member of the ‘trefoil’ family of cysteine-rich secreted proteins, is described. Analysis of recombinant pNR-2/pS2 by ion-exchange chromatography showed that it was heterogeneous. The heterogeneity was reduced by treatment with thiol-group-containing reagents, suggesting that it is caused by the odd number of cysteine residues in mature pNR-2/pS2, and this view was reinforced by mutation of the extra-trefoil domain cysteine residue, Cys58, to a serine residue. Electrophoresis of recombinant pNR-2/pS2 Cys58 and pNR-2/pS2 Ser58 proteins under non-denaturing conditions confirmed that the Ser58 mutant is much more homogeneous, and showed that most of pNR-2/pS2 Ser58 co-migrates as a single band with pNR-2/pS2 secreted from breast-cancer cells in culture. Treatment of recombinant pNR-2/pS2 proteins with various thiol-group-reactive reagents indicated that cysteine is the most effective at producing recombinant pNR-2/pS2 that co-migrates with pNR-2/pS2 secreted by breast-cancer cells. Dithiothreitol appeared to denature the proteins, and GSH was relatively ineffective. pNR-2/pS2 Cys58 treated with cysteine and untreated pNR-2/pS2 Ser58 had the same apparent molecular mass, measured by gel filtration, as pNR-2/pS2 secreted from breast-cancer cells. This is the first report of the production of a recombinant mature single-domain trefoil peptide and should greatly facilitate elucidation of the structure and function of pNR-2/pS2.
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Hill, M. G. "Does Psa affect kiwifruit susceptibility to leafrollers." New Zealand Plant Protection 66 (January 8, 2013): 162–69. http://dx.doi.org/10.30843/nzpp.2013.66.5587.
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Analysis of kiwifruit industry pest monitoring and spray diary data showed increases in the incidence of leafroller larvae on fruit during crop monitoring in the 2011/12 season in regions with the virulent strain of the disease Pseudomonas syringae pv actinidiae (PsaV) Four factors were considered as explanations PsaV infection and its effects on plant defences leafroller sprays PsaV sprays and leafroller sampling The most plausible cause of the increased leafroller incidence is PsaV infection A putative mechanism is hormonal crosstalk resulting in a tradeoff between pest and pathogen resistance The susceptibility of vines to leafrollers in PsaVinfected regions appears to persist for several weeks longer after fruit set than in uninfected vines The paper discusses the need to develop a better understanding of the interactions of biotic and abiotic stressors on plant defence responses to pests and diseases and to incorporate these into a plantdefencecentred approach to Integrated Pest Management
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Katagiri, Takayuki, Kohei Fujita, Shusaku Ikeyama, and Yutaka Amao. "Visible light-induced reduction system of diphenylviologen derivative with water-soluble porphyrin for biocatalytic carbon–carbon bond formation from CO2." Pure and Applied Chemistry 90, no. 11 (November 27, 2018): 1723–33. http://dx.doi.org/10.1515/pac-2018-0402.
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Abstract From the view point of green chemistry, CO2 utilization technologies with solar energy including the photoredox system have been received a lot of attention. As one of them, photoredox system containing a photosensitizer and a catalyst catalyzing a reaction of a carbon–carbon bond formation from CO2 as a feed stock were constructed. In a recent study, we reported the visible light-induced malate (C4 compound) production from pyruvate (C3 compound) and CO2 due to carbon–carbon bond formation with the system consisting an electron donor, a photosensitizer, diphenylviologen (PV2+) derivative as an electron mediator in the presence of malic enzyme (ME). However, the interaction between a photosensitizer and PV2+ derivative has not been clarified yet. In this study, water-soluble PV2+ derivative, 1,1′-bis(p-sulfonatophenyl)-4,4′-bipyridinium salt (PSV2+) was synthesized, and its electro-, photochemical properties were evaluated. Moreover, the photoredox properties of PSV2+ with water-soluble Zn porphyrin were studied using fluorescence spectroscopy and steady state irradiation. The fluorescence of Zn porphyrin was quenched by PSV2+ and the two-electron reduced form of PSV2+ were produced with Zn porphyrin with steady state irradiation. In addition, reaction solution containing triethanolamine, tetraphenylporphyrin tetrasulfonate, pyruvate, ME, Mg2+ and PSV2+ in CO2 saturated bis-tris buffer (pH 7.4) was irradiated with visible light, the oxaloacetate and malate were produced. This result indicates that PSV2+ is an efficient electron mediator in the visible light-induced redox system for carbon–carbon bond formation with ME from CO2 as a feedstock.