Dissertations / Theses on the topic 'Proximity analysis'
To see the other types of publications on this topic, follow the link: Proximity analysis.
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Proximity analysis.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
1
Nong, Rachel Yuan. "Proximity Ligation Assays for Disease Biomarkers Analysis." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-158634.
Full textAbstract:
One of the pressing needs in the field of disease biomarker discovery is new technologies that could allow high performance protein analysis in different types of clinical material, such as blood and solid tissues. This thesis includes four approaches that address important limitations of current technologies, thus enabling highly sensitive, specific and parallel protein measurements. Paper I describes a method for sensitive singleplex protein detection in complex biological samples, namely solid phase proximity ligation assay (SP-PLA). SP-PLA exhibited improved sensitivity compared to conventional sandwich immunoassays. We applied SP-PLA to validate the potential of GDF-15 as a biomarker for cardiovascular disease. Paper II describes ProteinSeq, a multiplexed immunoassay based on the principle of SP-PLA, for parallel detection of 36 proteins using next-generation sequencing as readout. ProteinSeq exhibited improved sensitivity compared to multiplexed sandwich immunoassays, and the potential to achieve even higher levels of multiplexing while preserving a high sensitivity and specificity. We applied ProteinSeq to analyze 36 proteins, including one internal control, in 5 μl of plasma samples in a cohort of patients with cardiovascular disease and healthy controls. Paper III describes PLA-DTM, a strategy for recording all possible interactions between sets of proteins in clinical samples. Individual proteins and their interactions are first encoded to dual barcoded DNA by PLA, and the barcodes are interrogated by a method named dual tag microarray (DTM). We applied the method for studying interactions among protein members of the NFκB signaling pathway. Paper IV describes a novel probing strategy for analyzing individual biomolecules in solution or in situ. The technique employs a new class of probes for unfolding proximity ligation assays - uPLA probes. The probes are designed so that each probe set is sufficient in forming and replicating circular DNA reporter, without interactions among themselves when incubated with the sample. The uPLA probing strategy provides ease in the design of multiple probe sets in parallelized assays while enhancing the specificity of detection. We used the uPLA probes to detect various targets, including synthetic DNA and cancer-related transcripts in situ.
2
Huaytia, Fernandez Ricardo. "Skin and proximity effect analysis of traction motor." Thesis, KTH, Elektrisk energiomvandling, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-96302.
Full textAbstract:
Traction motors are thermally pressed to obtain a high torque per unit volume at the same time as a relatively high frequency is used. Because of the high frequency the phenomenon skin effect and proximity effects appear, this is explored in the thesis. To find a link between the parasitic and thermal effects on the insulation is important. In this thesis, a finite element model of the Flux 2D suitable for skin effect analysis is developed; also a thermal network has been developed to find thermal hotspots in the stator slot. In this thesis, sinusoidal supplies for two operating points are studied and triangular sinusoidal PWM supply is as well studied for the nominal operating point.
3
Gokce, Mersin. "Coupled stability analysis of close proximity ship towing." Thesis, Monterey California. Naval Postgraduate School, 2002. http://hdl.handle.net/10945/6060.
Full textAbstract:
Approved for public release, distribution is unlimitedThe scope of this thesis is to study the stability of two ships in close proximity towing. Unlike previous studies in the past, the lateral dynamics of both ships are included in the formulation. The equations of motion of the system consist of the sway and yaw motions of the two ships and a control law for the leading ship. An eigenvalue stability analysis of the coupled system confirms the results that are obtained through numerical simulations. It is shown that it is possible for the system to be unstable even through the classical criteria for the towing stability are satisfied. A series of parametric studies is conducted in order to analyze the sensitivity of the system for different towline lengths, tension, and control time constant.
4
Fredriksson, Simon. "Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders." Doctoral thesis, Uppsala University, Department of Genetics and Pathology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2691.
Full textAbstract:
A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. As pairs of so-called proximity probes bind the individual target protein molecules at distinct sites, these reagents are brought in close proximity. The probes consist of a protein specific binding part coupled to an oligonucleotide with either a free 3’- or 5’-end capable of hybridizing to a common connector oligonucleotide. When the probes are in proximity, promoted by target binding, then the DNA strands can be joined by enzymatic ligation. The nucleic acid sequence that is formed can then be amplified and quantitatively detected in a real-time monitored polymerase chain reaction. This convenient assay is simple to perform and allows highly sensitive protein detection. Parallel analysis of multiple proteins by DNA microarray technology is anticipated for proximity ligation and enabled by the information carrying ability of nucleic acids to define the individual proteins. Assays detecting cytokines using SELEX aptamers or antibodies, monoclonal and polyclonal, are presented in the thesis.
Microarrays synthesized in situ using photolithographic methods generate impure products due to damaged molecules and interrupted synthesis. Through a molecular inversion mechanism presented here, these impurities may be removed. At the end of synthesis, full-length oligonucleotides receive a functional group that can then be made to react with the solid support forming an arched structure. The 3’-ends of the oligonucleotides are then cleaved, removing the impurities from the support and allowing the liberated 3’-hydroxyl to prime polymerase extension reactions from the inverted oligonucleotides. The effect of having pure oligonucleotides probes compared to ones contaminated with shorter variants was investigated in allele specific hybridization reactions. Pure probes were shown to have greater ability to discriminate between matched and singly mismatched targets at optimal hybridization temperatures.
5
Gu, Gucci Jijuan. "Proximity Ligation Assay for High Performance Protein Analysis in Medicine." Doctoral thesis, Uppsala universitet, Institutionen för immunologi, genetik och patologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-179827.
Full textAbstract:
High quality reagents are preconditions for high performance protein analyses. But despite progress in some techniques, e.g. mass spectrometry, there is still a lack of affinity-based detection techniques with enhanced precision, specificity, and sensitivity. Building on the concept of multiple affinity recognition reactions and signal amplification, a proximity ligation assay (PLA) was developed as a molecular tool for analyzing proteins and their post-translational modification and interactions. PLA enhanced the analysis of protein expression levels and post-translational modifications in western blotting (Paper I), which had elevated sensitivity and specificity, and an ability to investigate protein phosphorylation. A general and straightforward method was established for the functionalization of affinity reagents through adding DNA strands to protein domains for protein analysis in medicine (Paper II). A method for protein domain-mediated conjugation was developed to simplify the use of recombinant affinity reagents, such as designed ankyrin repeat protein (DARPin), in DNA-mediated protein analyses. Alzheimer’s disease (AD) is characterized by progressive cognitive decline and memory impairment, and amyloid-beta plaques and neurofibrillary tangles (NFT) in the brain are clinical hallmarks of the disease. In order to understand the mechanisms underlying the formation of NFT, in situ PLA was used to explore the role of microtubule affinity related kinase 2 (MARK2) in phosphorylating tau protein during the pathological progress of AD (Paper III). The analyses of roles of MARK proteins 1-4 in phosphorylating tau protein in cells and in post-mortem human brains were performed in Paper IV. The focus of this thesis was the study of post-translational modifications and interactions of proteins in medicine. Procedures for high performance protein analysis in western blotting via proximity ligation were developed, and a functionalization method for recombinant affinity reagents in DNA-mediated protein analysis was established. These and other techniques were used to investigate the roles of tau-phosphorylating MARK family proteins in AD.
6
Leuchowius, Karl-Johan. "High Content Analysis of Proteins and Protein Interactions by Proximity Ligation." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-119530.
Full textAbstract:
Fundamental to all biological processes is the interplay between biomolecules such as proteins and nucleic acids. Studies of interactions should therefore be more informative than mere detection of expressed proteins. Preferably, such studies should be performed in material that is as biologically and clinically relevant as possible, i.e. in primary cells and tissues. In addition, to be able to take into account the heterogeneity of such samples, the analyses should be performed in situ to retain information on the sub-cellular localization where the interactions occur, enabling determination of the activity status of individual cells and allowing discrimination between e.g. tumor cells and surrounding stroma. This requires assays with an utmost level of sensitivity and selectivity. Taking these issues into consideration, the in situ proximity-ligation assay (in situ PLA) was developed, providing localized detection of proteins, protein-protein interactions and post-translational modifications in fixed cells and tissues. The high sensitivity and selectivity afforded by the assay's requirement for dual target recognition in combination with powerful signal amplification enables visualization of single protein molecules in intact single cells and tissue sections. To further increase the usefulness and application of in situ PLA, the assay was adapted to high content analysis techniques such as flow cytometry and high content screening. The use of in situ PLA in flow cytometry offers the possibility for high-throughput analysis of cells in solution with the unique characteristics offered by the assay. For high content screening, it was demonstrated that in situ PLA can enable cell-based drug screening of compounds affecting post-translational modifications and protein-protein interactions in primary cells, offering superior abilities over current assays. The methods presented in this thesis provide powerful new tools to study proteins in genetically unmodified cells and tissues, and should offer exciting new possibilities for molecular biology, diagnostics and drug discovery.
7
de, Oliveira Felipe Marques Souza. "Development and Application of Proximity Assays for Proteome Analysis in Medicine." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-334536.
Full textAbstract:
Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. In paper I, we present an adaptation of the solid-phase proximity ligation assay (SP-PLA) for the detection of post-translational modification of proteins (PTMs). The assay was adapted for the detection of two of the most commons PTMs present in proteins, glycosylation and phosphorylation, offering the encouraging prospect of using detection of PTMs in a diagnostic or prognostic capacity. In paper II, we developed a variant of the proximity ligation assay using micro titer plate for detection and quantification of protein using optical density as readout in the fluorometer, termed PLARCA. With a detection limit considerably lower than ELISA, PLARCA detected femtomolar levels of these proteins in patient samples. In paper III, we aim to compare detection values of samples collected from earlobe capillary, venous plasma, as well as capillary plasma stored in dried plasma spots (DPS) assessed with a 92-plex inflammation panel using multiplex proximity extension assay (PEA). Despite the high variability in protein measurements between the three sample sources, we were able to conclude that earlobe capillary sampling is a suitable less invasive alternative, to venipuncture. In paper IV, we describe the application of PLARCA and proximity extension assay (PEA) for the detection of GAD65 autoantibodies (GADA). Thus, offering highly sensitive and specific autoimmunity detection.
8
Okan, Orhan Barbaros. "A design procedure for seakeeping analysis of close proximity ship towing." Thesis, Monterey, Calif. Naval Postgraduate School, 2002. http://hdl.handle.net/10945/6039.
Full textAbstract:
Approved for Public Release; Distribution is Unlimited.The purpose of this thesis is to develop an efficient analysis and design procedure for assessing the seakeeping behavior of surface ships in close proximity towing. The problem is formulated by using the heave and pitch equations of motion in regular waves. The vertical plane relative motions between the trailing and the leading ships are matched through the speed-resistance characteristics of the trailing ship. A sea state degradation factor is introduced. This factor characterizes the expected seakeeping performance penalty resulting from the connection. A series of parametric studies is conducted for various geometric properties and environmental characteristics. The results can be used to evaluate the response of the system and provide insight into parameter selection for motion minimization.
9
Nelson, Evan, Charles D. Creusere, Thomas Critz, and Eric Butcher. "Analysis of Communication Rates in the Proximity of Near-Earth Asteroids." International Foundation for Telemetering, 2013. http://hdl.handle.net/10150/579571.
Full textAbstract:
ITC/USA 2013 Conference Proceedings / The Forty-Ninth Annual International Telemetering Conference and Technical Exhibition / October 21-24, 2013 / Bally's Hotel & Convention Center, Las Vegas, NVIn this paper we analyze fundamental local-area communication issues related to proximity operations around near-earth asteroids. We are motivated by NASA's plan to send robotic spacecraft to numerous such asteroids in the coming years in preparation for an eventual manned mission. We consider here the case where multiple probes are deposited on the surface of an asteroid and must communicate the data they collect to each other and to earth by using the orbiting `mothership' as a relay. With respect to this scenario, we statistically analyze the ability of surface probes in various locations to communicate with the mothership as well as their abilities to network with one another. For the purposes of this analysis, we assume the simplest possible communications scenario: a surface probe can communicate with the mothership only when it has an unobstructed line of sight. At the frequencies of interest here, line of sight is a necessary condition but it is obviously not sufficient - the end-to-end link margins of our communications system must be high enough to support the desired/required data rates. The work presented in this paper extends our previous research in which we only analyzed the visibility of the locations on the surface of the asteroid. Here, we consider how visibility affects the required communications bandwidth and buffer sizes for both surface-to-spacecraft and surface-to-surface scenarios.
10
Creusere, Charles D., Evan Nelson, Thomas Critz, and Eric Buther. "Analysis of Communication Interconnectedness in the Proximity of Near-Earth Asteroids." International Foundation for Telemetering, 2012. http://hdl.handle.net/10150/581638.
Full textAbstract:
ITC/USA 2012 Conference Proceedings / The Forty-Eighth Annual International Telemetering Conference and Technical Exhibition / October 22-25, 2012 / Town and Country Resort & Convention Center, San Diego, CaliforniaIn this paper we analyze fundamental local-area communication issues related to proximity operations around near-earth asteroids. We are motivated by NASA's plan to send robotic spacecraft to numerous such asteroids in the coming years in preparation for an eventual manned mission. We consider here the case where multiple probes are deposited on the surface of an asteroid and must communicate the data they collect to each other and to earth by using the orbiting `mothership' as a relay. With respect to this scenario, we statistically analyze the ability of surface probes in various locations to communicate with the mothership as well as their abilities to network with one another. For the purposes of this analysis, we assume the simplest possible communications scenario: a surface probe can communicate with the mothership only when it has an unobstructed line of sight. At the frequencies of interest here, line of sight is a necessary condition but it is obviously not sufficient - the end-to-end link margins of our communications system must be high enough to support the desired/required data rates. Nonetheless, this simplistic analysis represents the first step in characterizing the communication system requirement for the asteroid-local portion of the system.
11
Rusch, Thomas, Patrick Mair, and Kurt Hornik. "COPS Cluster Optimized Proximity Scaling." WU Vienna University of Economics and Business, 2015. http://epub.wu.ac.at/4465/1/COPS.pdf.
Full textAbstract:
Proximity scaling (i.e., multidimensional scaling and related methods) is a versatile statistical
method whose general idea is to reduce the multivariate complexity in a data set by employing suitable proximities between the data points and finding low-dimensional
configurations where the fitted distances optimally approximate these proximities. The ultimate goal, however, is often not only to find the optimal configuration but to infer statements about the similarity of objects in the high-dimensional space based on the the similarity in the configuration. Since these two goals are somewhat at odds it can happen that the resulting optimal configuration makes inferring similarities rather difficult. In that case the solution lacks "clusteredness" in the configuration (which we call "c-clusteredness"). We present a version of proximity scaling, coined cluster optimized
proximity scaling (COPS), which solves the conundrum by introducing a more clustered appearance into the configuration while adhering to the general idea of multidimensional scaling. In COPS, an arbitrary MDS loss function is parametrized by monotonic transformations
and combined with an index that quantifies the c-clusteredness of the solution. This index, the OPTICS cordillera, has intuitively appealing properties with respect to measuring c-clusteredness. This combination of MDS loss and index is called "cluster optimized loss" (coploss) and is minimized to push any configuration towards a more clustered appearance. The effect of the method will be illustrated with various examples: Assessing
similarities of countries based on the history of banking crises in the last 200 years, scaling Californian counties with respect to the projected effects of climate change and their social vulnerability, and preprocessing a data set of hand written digits for subsequent classification by nonlinear dimension reduction. (authors' abstract)Series: Discussion Paper Series / Center for Empirical Research Methods
12
Clausson, Carl-Magnus. "Making Visible the Proximity Between Proteins." Doctoral thesis, Uppsala universitet, Science for Life Laboratory, SciLifeLab, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-217772.
Full textAbstract:
Genomic DNA is the template of life - the entity which is characterized by a self-sustaining anatomical development, regulated signaling processes, the ability to reproduce and to respond to stimuli. Through what is classically known as the central dogma, the genome is transcribed into mRNA, which in turn is translated into proteins. The proteins take part in most, if not all, cellular processes, and it is by unraveling these processes that we can begin to understand life and disease-causing mechanisms. In vitro and in vivo assays are two levels at which protein communication may be studied, and which permit manipulation and control over the proteins under investigation. But in order to retrieve a representation of the processes as close to reality as possible, in situ analysis may instead be applied as a complement to the other two levels of study. In situ PLA offers the ability to survey protein activity in tissue samples and primary cell lines, at a single cell level, detecting single targets in their natural unperturbed environment. In this thesis new developments of the in situ PLA are described, along with a new technique offering in situ enzyme-free detection of proximity between biomolecules. The dynamic range of in situ PLA has now been increased by several orders of magnitude to cover analogous ranges of protein expression; the output signals have been modified to offer a greater signal-to-noise ratio and to limit false-positive-rates while also extending the dynamic range further; simultaneous detection of multiple protein complexes is now possible; proximity-HCR is presented as a robust and inexpensive enzyme-free assay for protein complex detection. The thesis also covers descriptions on how the techniques may be simultaneously applied, also together with other techniques, for the multiple data-point acquisition required by the emerging realm of systems biology. A future perspective is presented for how much more information may be simultaneously acquired from tissue samples to describe biomolecular interactions in a new manner. This will allow new types of biomarkers and drugs to be discovered, and a new holistic understanding of life.
13
Lagarde, Ethan. "Using Suitability and Proximity Analysis to Discover Houston's Accessibility via Roadways and Public Transportation." Thesis, The University of Arizona, 2015. http://hdl.handle.net/10150/613073.
Full textAbstract:
Houston is one of the fastest growing metropolis’ in the country. Driving this growth is the oil and gas industry and also the Texas Medical Center, the world’s largest medical center. With such growth comes various problems. One of the leading problems according to its citizens in 2014 was traffic and the lack of access to public transportation.
This project aims to help find solutions to this problem by locating areas that could help improve public transportation access and take a look at Houston’s accessibility via roadways. Using datasets from various Houston agencies such as the City of Houston and the Houston-Galveston Area Council, overlay analysis was used to help find prime areas that could be improved. Using ESRI ArcMap, models were completed in order to automate the analysis process. Tools such as raster conversion, Euclidean distance, zonal stats as table, and reclassify were used. In order to analyze Houston accessibility via the roadways, ArcGIS Online was used. Several Proximity analyses were run in order to view various types of dating dealing with the accessibility of Houston using roadways.
The results show areas that do not currently have access to public transportation and areas that would be suitable locations for improvements based on different criteria. For roadway access, the results show average commute times, drive-time accessibility, and freeway access. This will allow for the accessibility of Houston to be shown whether it is by public transportation or by roadway.
14
Peper, Jonas [Verfasser]. "Proximity and Affinity based Analysis of Cardiac Caveolin Protein Interactions / Jonas Peper." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1228364419/34.
Full text
15
Li, Chi. "Impedance-Based Stability Analysis in Power Systems with Multiple STATCOMs in Proximity." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/85053.
Full textAbstract:
Multiple STATCOM units in proximity have been adopted in power transmission systems in order to obtain better voltage regulation and share burdens. Throughout stability assessment in this dissertation, it is shown, for the first time, that STATCOMs could interact with each other in a negative way in the small-signal sense due to their control, causing voltage instability, while loads and transmission lines showed small effects. Since this voltage stability problem is induced by STATCOMs, d-q frame impedance-based stability analysis was used, for the first time, to explore the inherent power system instability problem with presence of STATCOMs as it provides an accurate understanding of the root cause of instability within the STATCOM control system.
This dissertation first proposes the impedance model in d-q frame for STATCOMs, including dynamics from synchronization, current and voltage loops and reveals the significant features compared to other types of grid-tied converters that 1) impedance matrix strongly coupled in d and q channel due to nearly zero power factor, 2) different behaviors of impedances at low frequency due to inversed direction of reactive power and 3) coupled small-signal propagation paths on the voltage at point of common coupling from synchronization and ac voltage regulation.
Using the proposed impedance model, this dissertation identifies the frequency range of interactions in a viewpoint of d-q frame impedances and pinpointed that the ac voltage regulation was the main reason of instability, masking the effects of PLL in power transmission systems. Due to the high impedance of STATCOMs compared to that of transmission lines around the frequency range of interactions, STATCOMs were seen to interact with each other through the transmission lines. A scaled-down 2-STATCOM power grid was built to verify the conclusions experimentally.Ph. D.
16
Figueiro, Thiago Rosa. "Modélisation des procédés pour la correction des effets de proximity en lithographie électronique." Thesis, Université Grenoble Alpes (ComUE), 2015. http://www.theses.fr/2015GREAT011/document.
Full textAbstract:
Depuis l'apparition du premier circuit intégré, le nombre de composants constituant une puce électronique n'a cessé d'augmenter tandis que les dimensions des composants ont continuellement diminué. Pour chaque nouveau nœud technologique, les procédés de fabrication se sont complexifiés pour permettre cette réduction de taille. L'étape de lithographie est une des étapes la plus critique pour permettre la miniaturisation. La technique de lithographie qui permet la production en masse est la lithographie optique par projection. Néanmoins cette technologie approche de ses limites en résolution et l'industrie cherche de nouvelles techniques pour continuer à réduire la taille des composants. Les candidats sont l'écriture en plusieurs passes, la lithographie EUV, l'écriture directe, la nano-impression ou l'auto-organisation dirigée. Même si ces alternatives reposent sur des principes très différents, chacune a en commun l'utilisation de la lithographie électronique à un moment ou à un autre de leur réalisation. La lithographie électronique est sujette à des phénomènes spécifiques qui impactent la résolution finale, tels la diffusion des électrons, le « fogging », la diffusion d'acide, la CMP etc… La solution choisie par l'industrie pour tenir compte de tous ces phénomènes est de les prévoir puis de les compenser. Cette correction nécessite de les prédire à l'aide de modélisation, la précision de ces modèles décrivant les procédés étant primordiale. Dans cette thèse, les concepts de base permettant de développer un modèle sont présentés. L'évaluation de la qualité des données, la méthodologie de choix d'un modèle ainsi que la validation de ce model sont introduites. De plus, les concepts d'analyse de sensibilité locale et globale seront définis. L'état de l'art des stratégies utilisées ou envisagées pour les procédés lithographiques actuels ou futurs sont énoncés, chacune des principales étapes lithographiques étant détaillée. Les modèles tenant compte de la physique et de la chimie impactant sur la résolution après écriture par e-beam sont étudiés. De plus, les modèles compacts permettant de prédire les résultats obtenus par e-beam seront détaillés, pour finalement décrire les limitations des stratégies actuelles. De nouveaux modèles compactes sont proposés en introduisant de nouvelles familles de fonctions telles que les fonctions Gamma ou les fonctions de Voigt. De plus, l'utilisation des fonctions d'interpolations de type Spline sont également proposés. Un modèle résine d'utilisation souple a également été développé pour tenir compte de la plupart des comportements expérimentaux observés en évaluant les dimensions de motifs d'un dessin en utilisant des métriques appropriés. Les résultats obtenus en utilisant de telles méthodes montrent une amélioration de la précision de la modélisation, notamment en ce qui concerne les motifs critiques. D'autres modèles spécifiques permettant de décrire les effets d'extrême longue portée ou permettant de compenser les déviations entre deux procédés sont également décrits dans ce travail. Le choix du jeu de motifs de calibration est critique pour permettre à l'algorithme de calibration d'obtenir des valeurs robustes des paramètres du modèle. Plusieurs stratégies utilisées dans la littérature sont brièvement décrites avant l'introduction d'une technique qui utilise l'analyse de sensibilité globale basée sur la variance afin de sélectionner les types de géométries optimales pour la calibration. Une stratégie permettant la sélection de ces motifs de calibration est détaillée. L'étude de l'impact du procédé et des incertitudes de mesures issue de la métrologie est également abordée, ce qui permet d'énoncer les limites à attendre du modèle sachant que les mesures peuvent être imprécises. Finalement, des techniques permettant de s'assurer de la qualité d'un modèle sont détaillées, telle l'utilisation de la validation croisée. La pertinence de ces techniques est démontrée pour quelques cas réelSince the development of the first integrated circuit, the number of components fabricated in a chip continued to grow while the dimensions of each component continued to be reduced. For each new technology node proposed, the fabrication process had to cope with the increasing complexity of its scaling down. The lithography step is one of the most critical for miniaturization due to the tightened requirements in both precision and accuracy of the pattern dimension printed into the wafer. Current mass production lithography technique is optical lithography. This technology is facing its resolution limits and the industry is looking for new approaches, such as Multi-patterning (MP), EUV lithography, Direct Write (DW), Nano-imprint or Direct Self-Assembly (DSA). Although these alternatives present significant differences among each other, they all present something in common: they rely on e-beam writers at some point of their flow. E-beam based lithography is subject to phenomena that impact resolution such as are electron scattering, fogging, acid diffusion, CMP loading, etc. The solution the industry adopted to address these effects is to predict and compensate for them. This correction requires predicting the effects, which is achieved through modeling. Hence the importance of developing accurate models for e-beam process. In this thesis, the basic concepts involving modeling are presented. Topics such as data quality, model selection and model validation are introduced as tools for modeling of e-beam lithography. Moreover, the concepts of local and global sensitivity analysis were also presented. Different strategies of global sensitivity analysis were presented and discussed as well as one of the main aspects in its evaluation, which is the space sampling approach. State-of-the-art strategies for todays and future lithography processes were presented and each of their main steps were described. First Principle models that explain the physics and chemistry of the most influential steps in the process resolution were also discussed. Moreover, general Compact models for predicting the results from e-beam lithography were also presented. Finally, some of the limitations of the current approach were described. New compact models described as Point-Spread-Function (PSF) are proposed based on new distributions, such as Gamma and Voigt. Besides, a technique using Splines for describing a PSF is also proposed. Moreover, a flexible resist model able to integrate most of the observed behavior was also proposed, based on evaluating any pattern on the layout using metrics. Results using such method further improved the any of the PSF distribution approach on the critical features that were limiting the future technology nodes. Other specific models and strategies for describing and compensating for extreme-long-range effects and for matching two different fabrication processes are also proposed and described in this work. The calibration layout is a key factor for providing the calibration algorithm with the experimental data necessary to determine the values of each of the parameters of the model. Several strategies from the literature were briefly described before introducing one of the main propositions of this thesis, which is employing variance-based global sensitivity analysis to determine which patterns are more suitable to be used for calibration. A complete flow for selecting patterns for a calibration layout was presented. A study regarding the impact of process and metrology variability over the calibration result was presented, indicating the limits one may expect from the generated model according to the quality of the data used. Finally, techniques for assuring the quality of a model such as cross-validation were also presented and demonstrated in some real-life situations
17
Anbumony, Kasi Lakshman Karthi Lee Soo-Young. "Analysis and correction of three-dimensional proximity effect in binary E-beam nanolithography." Auburn, Ala., 2007. http://repo.lib.auburn.edu/2006%20Fall/Theses/ANBUMONY_KASI_19.pdf.
Full text
18
Wu, Di. "Proximity Ligation and Barcoding Assays : Tools for analysis of proteins and protein complexes." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-220070.
Full textAbstract:
Proteins are fundamental structural, enzymatic and regulatory components of cells. Analysis of proteins, such as by measuring their concentrations, characterizing their modifications, and detecting their interactions, provides insights in how biological systems work physiologically or pathologically at the molecular level. To perform such analysis, molecular tools with good sensitivity, specificity, high multiplexing and throughput capacity are needed. In this thesis, four different assays were developed and applied to detect and profile proteins and protein complexes in human body fluids, and in cells or tissues. These assays are based on targeting proteins or protein complexes by oligonucleotide-conjugated antibodies, and subsequent proximity dependent enzymatic reactions involving the attached DNA reporter sequences. In paper I, a solid-phase proximity ligation assay (SP-PLA) was applied to detect synthetic and endogenous amyloid beta protofibrils. The SP-PLA provided better sensitivity and increased dynamic range than a traditional enzyme-linked immunosorbent assay (ELISA). In paper II, in situ PLA was applied to investigate the correlation between MARK2-dependent phosphorylation of tau and Alzheimer’s disease. Greater numbers of MARK2-tau interactions and of phosphorylated tau proteins were observed in brain tissues from Alzheimer’s patients than in healthy controls. In paper III, a multiplex SP-PLA was applied to identify protein biomarker candidates in amyotrophic lateral sclerosis (ALS) disease and in the analgesic mechanism of spinal cord stimulation (SCS). Among 47 proteins in human cerebrospinal fluid (CSF) samples, four were found at significantly lower concentrations (p-values < 0.001) in the samples from ALS patients compared to those from healthy controls (follistatin, IL-1α, IL-1β, and KLK5). No significant changes of the analyzed proteins were found in the CSF samples of neuropathic pain patients in the stimulated vs. non-stimulated condition using SCS. In paper IV, a new technology termed the proximity barcoding assay (PBA) was developed to profile individual protein complexes. The performance of PBA was demonstrated on artificially assembled streptavidin-biotin oligonucleotide complexes. PBA was also proven to be capable of profiling transcriptional pre-initiation complexes from nuclear extract of a hepatic cell line.
19
Hung, Victoria. "Live cell proximity biotinylation enables proteomic analysis of mitochondria-endoplasmic reticulum contact sites." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/105026.
Full textAbstract:
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 2016.Cataloged from PDF version of thesis.
Includes bibliographical references.
The cell is able to carry out an astounding number of biological functions in part due to its ability to compartmentalize chemical reactions to different cellular regions and organelles. In order to understand the biological pathways that occur in a specific cellular region, one needs to know the identity of the protein players and how they behave spatially and temporally in the context of a living cell. Obtaining complete protein inventories is a recurring challenge in cell biology, and although mass spectrometry-based proteomics has made great strides in cataloging all proteins, traditional protocols are limited because they rely on a purification of the cellular entity of interest. This purification step is imperfect, and furthermore, many cellular regions cannot be purified. Prior work in the Ting lab developed a methodology to bypass organelle and complex purification by using an engineered ascorbate peroxidase (APEX) to tag proximal endogenous proteins with biotin for subsequent purification and identification by mass spectrometry. Although this method worked well in membrane-enclosed compartments, it was insufficiently specific when applied to unbounded or partially bounded regions such as the mitochondrial intermembrane space. This thesis presents a ratiometric tagging strategy that can achieve high spatial specificity in unbounded cellular regions. We use this strategy to map the proteomes of mitochondrial subcompartments to understand how the mitochondrion communicates with the rest of the cell, and in particular, with the endoplasmic reticulum. These proteomes led to the identification of a protein that may be a component of a tethering complex between mitochondria and the endoplasmic reticulum.
by Victoria Hung.
Ph. D.
20
Aldwihe, Ramez. "Computer vision for driving support systems: automatic traffic signs detection and proximity analysis." Master's thesis, Universidade de Évora, 2018. http://hdl.handle.net/10174/23063.
Full textAbstract:
The future of the automotive industry in the coming years will depend heavily on artificial intelligence techniques. This thesis proposes a technique for automatic detection and recognition of traffic signs from images, to provide a driver alert system. The system developed in this work includes algorithms to detect, classify and recognize traffic signs, based on a set belonging to a German database. The main signs are circular and triangular, which have two different colors, namely red and blue. Several examples of images, in different scenarios, are taken from the German roads, and are used to test the effectiveness of the proposed system. Traffic signs are detected by analyzing the color and shape information. The detected signs are classified accordingtotheCNNMachineLearningtechnique,andcanbeclassifiedinto43differentclassesaccording to previous classification already existing in the reference database. After detecting the presence of a traffic signs, the traffic signs is detected by comparing the traffic signs detected in the images with the signs in the database. The overall recognition accuracy is 75 % and processing is normally done in 1.6 seconds. This project is implemented with the OpenCV tool and the Python programming language; Sumário:
Visão Computacional aplicada a sistemas de apoio à condução: deteção automática de sinalização de trânsito e análise de proximidade
O futuro da indústria automóvel nos próximos anos irá depender fortemente das técnicas de inteligência artificial. Esta tese propõe uma técnica para a deteção automática e o reconhecimento de sinais de trânsito a partir de imagens, para proporcionar um sistema de alerta ao condutor. O sistema desenvolvido neste trabalho inclui algoritmos para detetar, classificar e reconhecer sinais de trânsito, nomeadamente um conjunto pertencente a uma base de dados alemã. Osprincipaissinaissãocircularesetriangulares,osquaistêmduascoresdiferentes,nomeadamentevermelho e azul. Vários exemplos de imagens, em diferentes cenários, são tirados das estradas alemãs, e são usados para testar a eficácia do sistema proposto. Os sinais de trânsito são detetados analisando a informação de cor e forma. Os sinais detetados são classificadosapartirdatécnicaCNNMachineLearning, podendoserclassificadosem43classesdiferentes, de acordo com classificação prévia já existente na base de dados de referência. Após deteção da presença de um sinal de trânsito, o reconhecimento do mesmo é feito comparando os sinais de trânsito detetados nas imagens com os sinais existentes na base de dados. Oacertodoreconhecimentogeraléde75%eoprocessamentoéfeitonormalmenteem1.6segundos. Este projeto for implementado com a ferramenta OpenCV e a linguagem de programação Python.
21
Anim-Appiah, Kofi D. "Analysis and design of ferrite cores for eddy current-killed oscillator inductive proximity sensors." Thesis, This resource online, 1991. http://scholar.lib.vt.edu/theses/available/etd-08252008-162029/.
Full text
22
Ebai, Tonge. "Development of Enhanced Molecular Diagnostic Tools for Protein Detection and Analysis." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-320380.
Full textAbstract:
Improved diagnosis, prognosis and disease follow-up is a fundamental procedure and a constant challenge in medicine. Among the different molecular biomarkers, proteins are the essential regulatory component in blood; hence, by developing enhanced specific and sensitive molecular tools will gives great insight into the different processes in disease treatment. In this thesis, we build on the proximity ligation assay to develop and apply new adaptable methods to facilitate protein detection. In paper I, I present a variant of the proximity ligation assay (we call PLARCA) using micro titer plate for detection and quantification of protein using optical density as readout in the fluorometer. PLARCA detected femtomolar levels of these proteins in patient samples, which was considerably below the detection threshold for ELISA. In paper II, we developed and adapted a new method into the in situ PLA methods for detection and identification of extracellular vesicles (EVs) using flow cytometry as readout (a method we call ExoPLA). We identified five target proteins on the surface of the Evs and using three colors, we identified the EV using flow cytometer. In paper III, we aim to improve the efficiency of in situ PLA by creating and developing new designs and versions of the assay we called Unfold probes Through comparison of detection of protein using in situ PLA versus Unfold probes, we observed considerable decrease in non-specific signals, and also a lower detection threshold. In paper IV, we describe the development of a solid phase proximity extension (sp-PEA) assay for protein detection and quantification. We compared detection of IL-8, TNF-alpha, IL-10 and IL-6 using spPEA and PEA; spPEA demonstrations over 2 orders of magnitudes in the lower detection concentrations by decreased in background noise.
23
Furuhashi, Takeshi, Tomohiro Yoshikawa, and Minh Tuan Pham. "Questionnaire Data Analysis Based on a New Dendrogram for Visualization of Hierarchical Structure of Proximity." Korean Institute of Intelligent Systems, 2009. http://hdl.handle.net/2237/20684.
Full text
24
Arngården, Linda. "Analysis of signaling pathway activity in single cells using the in situ Proximity Ligation Assay." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-281716.
Full textAbstract:
A cell that senses signals from its environment uses proteins for signal transduction via post translational modifications (PTMs) and protein- protein interactions (PPIs) from cell membrane into the nucleus where genes controlling cell proliferation, differentiation and apoptosis can be turned on or off, i.e. changing the phenotype or fate of the cell. Aberrations within such proteins are prone to cause diseases, such as cancer. Therefore, it is important so study aberrant signaling to be able to understand and treat diseases. In this thesis, signaling aberrations of PTMs and PPIs were analyzed with the use of the in situ proximity ligation assay (in situ PLA), and the thesis also contain method development of rolling circle amplification (RCA), which is the method used for signal amplification of in situ PLA reaction products. Paper I considers the integrity of RCA products. Here, the aim was to generate a smaller and more compact RCA product, for more accurate either visual or automated analysis. This was achieved with the use of an additional so called compaction oligonucleotide that during RCA was able to bind and pull segments of RCA products closer together. The compaction oligonucleotide served to increase the signal to noise ratio and decrease the number of false positive signals. The crosstalk between the Hippo and TGFβ signaling pathways were studied in paper II. Activity of the Hippo signaling pathway is regulated by cell density sensing and tissue control. We found differences in amounts and localization of interactions between the effector proteins of the two pathways depending on cell density and TGFβ stimulation. In paper III the NF-кB signaling pathway constitutively activated in chronic lymphocytic leukemia (CLL) was studied. A 4 base-pair frameshift deletion within the NFKBIE gene, which encodes the negative regulator IкBε, was found among 13 of a total 315 cases by the use of targeted deep sequencing. We found reduced levels of IкBε protein, decreased p65 inhibition, and increased phosphorylation, along with increased nuclear localization of p65 in NFKBIE deleted cases compared to healthy cases. Crosstalk between the Hippo and Wnt signaling pathway are studied within paper IV. Here, we found differences in cellular localization of TAZ/β-catenin interactions depending on colon cancer tumor stage and by further investigate Hippo/WNT crosstalk in cell line model systems we found an increase of complex formations involved in the crosstalk in sparse growing HEK293 cells compared to dense growing cells. Also, active WNT3a signaling was affected by cell density. Since cell density showed to have a big effect on Hippo/WNT crosstalk we continued to investigated the effect of E-cadherin, which has a function in cell junctions and maintenance of epithelial integrity on Hippo/WNT crosstalk. Interestingly, we found that E-cadherin is likely to regulate Hippo/WNT crosstalk.
25
ROSE, EMILY JESSICA. "AN ANALYSIS OF THE HEALTH OF GARDEN SOILS IN CLOSE PROXIMITY TO TUCSON INTERNATIONAL AIRPORT." Thesis, The University of Arizona, 2016. http://hdl.handle.net/10150/613569.
Full textAbstract:
There are many known health effects linked to lead exposure, such as impaired neurological development in children. Despite this, piston-engine airplanes still contain tetraethyl lead in their gasoline, as there is currently no safe alternative. Because it is known that close exposure to lead can influence the public health of the community, this study researches if there is a link between proximity to an airport and soil health in gardens. Five gardens near the Tucson International Airport were studied, along with two control gardens. The results indicate that there is no correlation between leaded gasoline in airplanes and lead contamination in garden soils, with each soil in the study containing between 7.19-12.99 milligrams per kilogram (mg/kg) lead. This is minimal, compared to the EPA's recommended exposure limit of 400 mg/kg lead and more stringent state guidelines of 100 mg/kg, indicating that there is not a strong public health impact. In addition, four other metals were analyzed. Beryllium was present in amounts of 0.24-0.41 mg/kg, zinc between 42.40-130.05 mg/kg, arsenic between 2.04-5.25 mg/kg, and cadmium between 0.17-1.04 mg/kg. All of these results were below recommended guidelines and were in the category of healthy, uncontaminated soils.
26
Walsh, Patrick. "Hedonic Property Value Modeling of Water Quality, Lake Proximity, and Spatial Dependence in Central Florida." Doctoral diss., University of Central Florida, 2009. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/3212.
Full textAbstract:
Hedonic property value analysis is one of the leading methods of environmental valuation. This non-market technique uses variation in home sales to infer the values of amenities or disamenities. While there have been numerous studies about air quality and hazardous waste, the number of papers focusing on water quality is much smaller. Consequently, there are still many unanswered questions about the proper handling of water quality through hedonic methods. Furthermore, estimates from hedonic property price analyses are rarely used in government cost benefit analyses. This dissertation investigates several important hedonic issues in a large analysis of water quality in central Florida. The first chapter of this paper explores the extent of water quality benefits. Almost all past studies have focused exclusively on waterfront homes. The present paper includes non-waterfront homes and investigates three hypotheses about the marginal impact of water quality. The first hypothesis is that non-waterfront homes are positively affected by water quality, but by a smaller amount than waterfront homes. The second hypothesis is about the effect of lake distance on the relationship between water quality and property prices: this relationship should be negative. The third hypothesis states that properties near larger lakes have a higher implicit price for water quality than homes around smaller lakes, all else constant. These three hypotheses are investigated in each chapter of the dissertation, and provide a unifying theme to the paper. Results from Chapter 1 support all three hypotheses. Most importantly, the empirical estimates indicate that water quality benefits extend beyond the waterfront in a declining gradient. Excluding non-lakefront homes from the analysis can therefore substantially underestimate the total benefits of a water quality improvement. Estimates of the total property price benefits from a one foot increase in water quality were found to double with the addition of non-waterfront homes. The second chapter examines the sensitivity of results to several spatial specifications. Spatial issues can be a problem in analyses of real estate data because of spatially correlated variables, unobservable neighborhood codes and covenants, identical or similar builders, and property appraisal valuation techniques. The focus of the chapter is on the spatial weights matrix (SWM). Six different SWM's are constructed, which are based on popular specifications encountered in the current spatial hedonic literature. An out-of-sample forecasting exercise is used to compare multiple spatial specifications. Results indicate that certain spatial models may be sensitive to the specification of the weights matrix. Furthermore, many popular models currently used in the literature could be improved by allowing more non-zero elements in the SWM. The third chapter investigates the definition of "water quality" and uses several additional quality indicators. Choosing the proper pollution indicator is an issue that has plagued many areas of the valuation literature. While clarity indicators have become popular in hedonic property price analysis, they are not used for the purposes of regulation by many state environmental departments. This chapter uses several indicators that are used by the state of Florida to classify lakes and implement policy. Implicit prices are computed for all of the indicators and issues of benefit extent and total benefits are explored. Instead of finding an optimal indicator for all situations, results indicate that the use of at least two types of indicators may capture a larger range of the true total benefits. The final chapter uses a repeat sales model to address potential problems with omitted variable bias. Due to the size of the data set in this paper, there are a substantial number of homes that have sold more than once. The repeat sales model analyzes differences in property sales prices for the same home over time. The three hypotheses of the first chapter are explored in this alternative model. The implicit price obtained from the repeat sales model is much larger than the regular hedonic model. However, there are some concerns with the smaller population of repeat sales.Ph.D.
Department of Economics
Business Administration
Economics PhD
27
Liang, Robert, Bruce Kwan, and Cedric Florens. "ON THROUGHPUT ANALYSIS OF THE MARS IN-SITU ARQ PROTOCOL." International Foundation for Telemetering, 2000. http://hdl.handle.net/10150/607709.
Full textAbstract:
International Telemetering Conference Proceedings / October 23-26, 2000 / Town & Country Hotel and Conference Center, San Diego, CaliforniaCombating harsh and unpredictable channel environments is a part of the design of any in-situ communication system (i.e. rover to lander, rover to orbiter, etc.). Channel characteristics can range from simple additive white Gaussian noise (AWGN) channels to more bursty fading channels found in rover to orbiter links (i.e. canyon scenarios and typical orbiter passes around mountain ranges). A combination of forward error correction and automatic repeat request (ARQ) schemes are commonly used to provide a more robust communications link. ARQ enhances the communication link particularly for bursty fading channels. Go-Back-N is a commonly used ARQ scheme and is an option in the newly developed Consultative Committee for Space Data Systems (CCSDS) Proximity-1 Link protocol [7], a data link layer protocol targeted specifically for in-situ applications. Optimization of frame sizes and retransmission persistence of the ARQ scheme require a good analytical model of how the scheme performs over various channel conditions. In this paper, an analytical framework for modeling the COP-1 protocol is presented for both AWGN channels along with bursty fading channels. A Gilbert-Elliot two-state Markov model is used to model a bursty fading channel.
28
Zieba, Agata. "Application of Proximity Ligation Assay for Multidirectional Studies on Transforming Growth Factor-β Pathway." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-171952.
Full textAbstract:
A comprehensive understanding of how the body and all its components function is essential when this knowledge is exploited for medical purposes. The achievements in biological and medical research during last decades has provided us with the complete human genome and identified signaling pathways that governs the cellular processes that facilitates the development and maintenance of higher order organisms. This has brought about the realization that diseases such as cancer is a consequence of genomic aberrations that effects these signaling pathways, endowing cancer cells with the capacity to circumvent homeostasis by acquiring features like self-sustained proliferation and insensitivity to apoptosis. The increased understanding of biology and medicine has been made possible by the development of advanced methods to carry out biological and clinical analyses. The demands of a method often differ regarding in what context it will be applied. It may be acceptable for method to be laborious and time consuming if it is used in basic research, but for medical purposes molecular methods need to be fast and straightforward to perform. Innovative technologies should preferentially address the demands of both researchers and clinicians and provide data not possible to obtain by other methods. An example of such a method is the in situ proximity ligation assay (in situ PLA). In this thesis I have used this method to determine the activity status, at the single-cell level, of the transforming growth factor-β (TGF-β) signaling pathway and activating protein-1 (AP-1) family of transcription factors. Both of these pathways are frequently involved in cancer development and progression. In addition to this research I herein also present further modifications of in situ PLA, and analyses thereof, to increase the utility and resolution of this assay.
29
Askarzadeh, Fardad. "Diffraction Analysis with UWB Validation for ToA Ranging in the Proximity of Human Body and Metallic Objects." Digital WPI, 2017. https://digitalcommons.wpi.edu/etd-dissertations/339.
Full textAbstract:
"The time-of-arrival (ToA)-based localization technique performs superior in line-of-sight (LoS) conditions, and its accuracy degrades drastically in proximity of micro-metals and human body, when LoS conditions are not met. This calls for modeling and formulation of Direct Path (DP) to help with mitigation of ranging error. However, the current propagation tools and models are mainly designed for telecommunication applications via focus on delay spread of wireless channel profile, whereas ToA-based localization strive for modeling of DP component. This thesis provides a mitigation to the limitation of existing propagation tools and models to computationally capture the effects of micro-metals and human body on ToA-based indoor localization. Solutions for each computational technique are validated by empirical measurements using Ultra-Wide-Band (UWB) signals. Finite- Difference-Time-Domain (FDTD) numerical method is used to estimate the ranging errors, and a combination of Uniform-Theory-of-Diffraction (UTD) ray theory and geometrical ray optics properties are utilized to model the path-loss and the ToA of the DP obstructed by micro- metals. Analytical UTD ray theory and geometrical ray optics properties are exploited to model the path-loss and the ToA of the first path obstructed by the human body for the scattering scenarios. The proposed scattering solution expanded to analytically model the path-loss and ToA of the DP obstructed by human body in angular motion for the radiation scenarios."
30
Stilwell, Justin Daniel Lawrence. "The importance of air transportation to the U.S. economy : analysis of industry use and proximity to airports." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/81129.
Full textAbstract:
Thesis (S.M. in Technology and Policy)--Massachusetts Institute of Technology, Engineering Systems Division, 2013.Cataloged from PDF version of thesis.
Includes bibliographical references (p. 109-113).
This thesis investigates broader impacts of air transportation on U.S. economic productivity, as well as market access and business location, in order to help identify how aviation supports the national economy. More traditional economic impacts are reviewed before turning to enabling impacts. Mechanisms by which air transportation might enhance economic productivity are proposed and a production model is constructed as a framework for exploring the validity of these mechanisms. Two analyses are conducted which should provide new insights to the FAA on the importance of air transportation to the U.S. economy. Focusing on the demand side of the economy, a detailed analysis of input-output (1-0) data from the Bureau of Economic Analysis (BEA) identifies where air transportation appears to be especially critical to economic production. On the supply side, U.S. Census Bureau data is used to map distributions of population, business establishments, and Fortune 500 headquarters from hub airports. Additional distribution analyses are performed for cargo airports and for select metropolitan areas. Analyses of intermediate use of air transportation provide weaker evidence than initially hypothesized as to aviation's role in supporting productivity growth. Both sets of analyses confirm that the importance of air transportation to industry is not uniform and that the government and services sectors appear to benefit from and take advantage of access to aviation more than other industry sectors. In particular, the analyses of business location relative to airports provide evidence that many service and high-value economic sectors are more concentrated near hub airports than are other industry sectors for which air transportation adds less value.
by Justin Daniel Lawrence Stilwell.
S.M.in Technology and Policy
31
Tulukcuoglu, Güneri Ezgi. "Development of microfluidic device for high content analysis of circulating tumor cells." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066583/document.
Full textAbstract:
Le cancer est l'une des principales causes de décès dans le monde. D'après la société américaine contre le cancer; en 2015, un quart des décès aux Etats-Unis est du au cancer du poumon avant même les maladies cardiaques. Cette situation nous incite et bien d'autres scientifiques dans le monde à développer des moyens plus efficaces de traitement, le diagnostic et le dépistage de la maladie. Parce que près de 90% des décès par cancer sont dus à des métastases, de nombreuses études se sont concentrées sur le mécanisme de métastases et sur son impact clinique. Les cellules tumorales circulantes (CTC) sont les cellules s’échappent de tumeurs primaires ou métastatiques pour rejoindre le flux sanguin périphérique, ces cellules sont un élément de transition dans le processus métastatique et portent ainsi des informations cruciales sur ce mécanisme encore mal compris. Les CTCs ont déjà montré leur potentiel comme biomarqueur de pronostic de la progression de la maladie et de l'indicateur de l'efficacité du traitement en fonction l’augmentation ou de la diminution de leur nombre. Leur caractérisation moléculaire peut également donner des informations vis à vis de cibles thérapeutiques possibles et des mécanismes de progression de la maladie ou de la résistance aux médicaments. Leur comptage au cours du traitement combiné avec leur caractérisation moléculaire devrait améliorer la prise en charge des patients dans le cadre de la médecine personnalisée. Cependant CTCs sont extrêmement rares, 1 à 10 cellules / ml de sang parmi les 106 globules blancs et 109 globules rouges, leur capture à partir du sang reste donc un challenge analytique. Dans les dernières décennies, Une grande variété de techniques d'enrichissement et de capture a été mise au point et l'approche microfluidique est l'une des méthodes efficaces, flexibles et à haut débit. Au sein de notre équipe, un dispositif microfluidique (système Ephesia) puissant pour la capture et l'analyse des cellules tumorales circulantes a déjà été mis au point précédemment. Le principe de capture est basé sur l'auto-assemblage de billes magnétiques greffées par des anticorps, grâce aux quelles les cellules sont enrichies via l’interaction Ab- l'antigène de surface EpCAM que l'on trouve communément dans les cellules cancéreuses d'origine épithéliale. Ce système a déjà été validé avec des lignées cellulaires et des échantillons de patients. Cependant, le système n'a pas permis l'isolement / détection des sous-populations de CTCs ou d'effectuer une caractérisation moléculaire très poussée. Par conséquent, mon projet de thèse vise à améliorer encore les capacités du système sur les deux principaux aspects: le ciblage sous-populations de CTC et à l'étude des interactions des protéines à la surface des CTCs dans le Système EphesiaMetastasis is the advanced stage of cancer progression and is the cause of 90% of deaths in cancer disease. During metastatic cascade, it is suggested that the successful metastatic initiation depends on the survival of circulating tumor cells (CTCs). CTCs are the cells that shed from the primary or secondary tumor sites into the blood circulation. it is now widely recognized as potential biomarker for companion diagnostics in which high number of CTCs in blood can indicate association with poor survival or high risk of disease progression. Besides, following the number of CTCs during the course of treatment can help to adapt the selected therapy and predict the treatment efficacy. On the other hand molecular characterization can provide patient stratification and identifying the therapeutic targets. However they are extremely rare in the bloodstream, estimated between 1-10 CTC among 6×106 leukocytes, 2×108 platelets and 4×109 erythrocytes per one mL of blood which makes their isolation very challenging. A very attractive way of isolation of CTCs is to integrate microfluidics. Microfluidics offers great advantages such as low volume of reagent consumption and short analysis times with automation as well as isolation and detection analysis can be integrated resulting in highly efficient biomedical devices for diagnostics. As parallel to state of the art, a powerful microfluidic device for circulating tumor cells capture and analysis had already been developed previously in our laboratory. The principle of capture is based on self-assembly of antibody-coated (EpCAM) magnetic beads in which the cells are enriched by EpCAM surface antigen which is found commonly in epithelial origin cancer cells. This system was already validated with cell lines and patients samples. However, the system did not allow isolation/detection of subpopulations of CTCs or performing high content molecular characterization. Therefore, my PhD project aimed at further improving the capabilities of the system on the main two aspects: targeting subpopulations of CTC and studying of protein interactions of CTCs in Ephesia System
32
Boyce, Jeremy Dewayne Tomasini Nathan. "An analysis of the NCAA Division III football playoff system and its use of the geographic proximity rule." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,1042.
Full textAbstract:
Thesis (M.A.)--University of North Carolina at Chapel Hill, 2007.Title from electronic title page (viewed Mar. 27, 2008). "... in partial fulfillment of the requirements for the degree of Master of Arts in the Department of Exercise and Sport Science Sport Administration." Discipline: Exercise and Sports Science; Department/School: Exercise and Sport Science.
33
Esparza, Timothy R. A. "Crafting clusters: an analysis of the craft beer industry in northern Colorado." Kansas State University, 2016. http://hdl.handle.net/2097/32643.
Full textAbstract:
Master of Regional and Community PlanningDepartment of Landscape Architecture/Regional and Community Planning
Katherine Nesse
The following report examines the craft beer industry as a major component of the beverage production cluster in Northern Colorado. By using the four locational determinants derived by Porter (1990) as a framework, this study evaluates the role of geography as a key component in an industry’s ability to foster a competitive advantage. Despite his focus on national competitiveness, Porter's diamond model has influenced strategic thinking on a regional scale (Stimson, Stough & Roberts, 2006). In turn, it can help us to understand the interactions that underlie localized cluster dynamics. The cluster conception in economic development literature assumes that each of Porter's components is equally spatially connected. Resources are focused towards building assets in a region defined by analyzing the cluster. However, factors of the craft beer industry in Northern Colorado did not completely adhere to the traditional parameters of regional cluster geography. Personal interviews with key actors involved in the craft beer industry, along with economic data revealed that local factors are not always the driving force behind the development of the craft beer industry. In addition, the data analysis indicates that determinants of cluster success may be significant at various geographic scales. Locational determinants may not operate within the same area as defined by cluster analysis. Thus, this report closes with a recommendation to consider the significance of proximity when looking to increase the competitiveness of a given industry cluster—for the relationship between locational determinants and geography varies between factors.
34
LaBouve, Eric. "Relevance Analysis for Document Retrieval." DigitalCommons@CalPoly, 2019. https://digitalcommons.calpoly.edu/theses/1976.
Full textAbstract:
Document retrieval systems recover documents from a dataset and order them according to their perceived relevance to a user’s search query. This is a difficult task for machines to accomplish because there exists a semantic gap between the meaning of the terms in a user’s literal query and a user’s true intentions. Even with this ambiguity that arises with a lack of context, users still expect that the set of documents returned by a search engine is both highly relevant to their query and properly ordered. The focus of this thesis is on document retrieval systems that explore methods of ordering documents from unstructured, textual corpora using text queries. The main goal of this study is to enhance the Okapi BM25 document retrieval model. In doing so, this research hypothesizes that the structure of text inside documents and queries hold valuable semantic information that can be incorporated into the Okapi BM25 model to increase its performance. Modifications that account for a term’s part of speech, the proximity between a pair of related terms, the proximity of a term with respect to its location in a document, and query expansion are used to augment Okapi BM25 to increase the model’s performance. The study resulted in 87 modifications which were all validated using open source corpora. The top scoring modification from the validation phase was then tested under the Lisa corpus and the model performed 10.25% better than Okapi BM25 when evaluated under mean average precision. When compared against two industry standard search engines, Lucene and Solr, the top scoring modification largely outperforms these systems by upwards to 21.78% and 23.01%, respectively.
35
Li, Xinbao Wilson. "The interaction effects of social presence, recipient availability, urgency, relationship, and proximity on media selection : a cost minimization analysis." HKBU Institutional Repository, 2004. http://repository.hkbu.edu.hk/etd_ra/520.
Full text
36
Almgren, Anders, and Viktor Haggren. "Market shares of regional shopping centres with proximity to an IKEA warehouse : IKEA Centres Case Study." Thesis, KTH, Fastigheter och byggande, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-211206.
Full textAbstract:
This master thesis focuses on regional shopping centre’s market shares. It is written in cooperation with IKEA Centres that provided data regarding their shopping centres and funds for the gathering of statistics. The research question for the study is: What level of market share is valid for regional shopping centres in close proximity to an IKEA warehouse? In order to answer this question as accurate as possible the study is designed as a case study. The case is implemented on three different shopping centres, owned by IKEA Centres, with similar locations and market areas. The three shopping centres that are selected as subject centres in the study are Birsta City in Sundsvall, I-Huset in Linköping and Erikslund Shopping Center in Västerås. The case is focused on the regional shopping centres isolated and do not include the IKEA furniture store. The first part of the study concerns the delineation of the subject centres’ primary- and secondary market area. This is done using Reilly’s Law based on the prerequisites of the specific shopping centre. By using this method breaking points or borders of the market areas can be defined and located based on the calculated driving time. To be able to calculate the potential market shares for the centres, the buying power segmentation method is used. The statistics are bought from Statistics Sweden and concerns mainly the number of households in the market areas and their disposable income. The market share is calculated by comparing the potential buying power of the households and the actual sales in the shopping centres. The results of the conducted study regarding the market share in the total market area is that Birsta City has a significant larger market share (60%) than the two other centres that the study concerns. I-Huset (17% market share) and Erikslund Shopping (25% market share) are located in regions with a higher population and more competition, the authors see this as the main factor to the difference in the market share. Results regarding market shares in different categories of goods are also presented. The three subjects’ centres offer several different collections of items. All centres have a large market share in the fashion segments that are offered, a wide tenant mix in combination with the target groups is seen to be an effecting factor.Denna masteruppsats fokuserar på ämnet regionala shoppingcenters och dess marknadsandel. Uppsatsen är skriven i samarbete med IKEA Centres vilka har bidragit med data angående köpcentren samt medel för inköp av statistik. Studiens frågeställning är: Vilken marknadsandel har regionala köpcenter där ett Ikeavaruhus finns i närheten? För att besvara denna frågeställning på bästa sätt är studien utformad som en fallstudie. Fallstudierna är implementerade på tre olika shopping center i liknande läge och med likvärdiga marknadsområden. De tre köpcentrumen vilket är utvalda som ämnescenter i studien är Birsta City i Sundsvall, I-Huset i Linköping och Erikslund Shopping Center i Västerås. Forskningen är baserad på shopping centret och berör inte IKEAs möbelvaruhus. Den första delen av studien behandlar avgränsningen av köpcentrumens primära och sekundära marknadsområden. Avgränsningen är utförd med Reilly’s Law baserad på de specifika köpcentrets förutsättningar. Genom att använda den här metoden definieras brytpunkter och gränser för marknadsområdet kan avgränsas med hjälp av den beräknade körtiden. För att sedan beräkna köpcentrets marknadsandel används metoden ”Buying power segmentation method”. Statistiken som används är köpt från SCB och berör huvudsakligen antalet hushåll i marknadsområdena samt dess disponibla inkomst. Marknadsandelen är beräknad genom att jämföra den potentiella köpkraften hos hushållen med den faktiska försäljningen i köpcentren. Resultatet av den utförda studien rörande marknadsandelar av den totala marknaden är dels att Birsta City har en betydligt större marknadsandel (60%) än de två andra undersökta köpcentren. I-Huset (17% marknadsandel) samt Erikslund Shopping (25% marknadsandel) är belägna i regioner med högre invånarantal och större konkurrens. Författarna ser detta som en avgörande faktor till skillnaden i marknadsandel jämfört med Birsta City. Resultat angående marknadsandelar i olika kategorier av varor är också presenterade. De tre studerade köpcentren erbjuder ett brett utbud av produkter. Samtliga center har en stor marknadsandel i kategorier rörande kläder & mode. Den breda hyresgästmixen samt målgruppen för köpcentren antas vara påverkande faktorer till detta.
37
Xu, Xingya. "The Effects of Anticipated Feedback Proximity on Performance: Exploring the Moderating Role of Self-Efficacy and Task Type." TopSCHOLAR®, 2015. http://digitalcommons.wku.edu/theses/1502.
Full textAbstract:
The present study investigated the effect of anticipated feedback proximity (immediately after completing the task or one week later) on performance and the moderating role of self-efficacy and task types (analytical or creative). I hypothesized that expecting rapid feedback should yield better performance than expecting delayed feedback, for people with high self-efficacy or those who receive analytical tasks. For those who receive creative tasks or have low self-efficacy, expecting rapid feedback may produce negative impact on performance. The results indicated a trend of main effect of anticipated feedback proximity. Specifically, participants performed better when they expected immediate feedback relative to expecting delayed feedback, regardless of the task type. There was also a main effect of task type. Performance on the analytical tasks was better than performance on the creative tasks. However, neither self-efficacy nor task type moderated the effects of anticipated feedback proximity. The implications for these findings are discussed herein.
38
Magdic, Matthew James. "Assessment of Soil Properties in Proximity to Abandoned Oil Wells usingRemote Sensing and Clay X-ray Analysis, Wood County, Ohio." Bowling Green State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1462537679.
Full text
39
Pienaar, D. van V. "Longitudinal stability and control analysis and parameter sensitivity investigation of fixed wing aircraft in ground proximity using various aerodynamic approaches." Doctoral thesis, University of Cape Town, 2000. http://hdl.handle.net/11427/5486.
Full textAbstract:
Summary in English.The investigation of the stability of longitudinal motion of fixed wing aircraft in ground proximity presented here focuses on rectangular wing and tail configurations similar to that of the Piper Cherokee PA-28-180. The majority of our results pertain to the height range down to one quarter of the wing span, and th main objectives of the investigation were the following: to reveal the physical nature and causes of the instability encountered in the close ground proximity; to assess the influence the design parameters such as the centre of the mass placement, tail length, tail height and wing aspect ration have on the stability of longitudinal motion; and to suggest a feedback control strategy that would alleviate the instability.
40
Mukherjee, Joyita [Verfasser]. "Identification and comparative analysis of the β-actin mRNA interactome by RNA-proximity labeling in mouse embryonic fibroblast / Joyita Mukherjee." Tübingen : Universitätsbibliothek Tübingen, 2020. http://d-nb.info/1219064599/34.
Full text
41
Frank, Sebastian [Verfasser], Arjan [Akademischer Betreuer] Kuijper, Dieter W. [Akademischer Betreuer] Fellner, and Laerhoven Kristof [Akademischer Betreuer] Van. "Analysis of capacitive proximity sensing as basis for human vehicle interfaces / Sebastian Frank ; Arjan Kuijper, Dieter W. Fellner, Kristof Van Laerhoven." Darmstadt : Universitäts- und Landesbibliothek, 2021. http://d-nb.info/1236344936/34.
Full text
42
Kawakami, Tetsu, and Eri Yamada. "Assessing Dynamic Externalities from a Cluster Perspective: The Case of the Motor Metropolis in Japan." 名古屋大学大学院経済学研究科附属国際経済政策研究センター, 2013. http://hdl.handle.net/2237/18512.
Full text
43
Razafindramanana, Octavio. "Low-dimensional data analysis and clustering by means of Delaunay triangulation." Thesis, Tours, 2014. http://www.theses.fr/2014TOUR4033/document.
Full textAbstract:
Les travaux présentés et discutés dans cette thèse ont pour objectif de proposer plusieurs solutions au problème de l’analyse et du clustering de nuages de points en basse dimension. Ces solutions s’appuyent sur l’analyse de triangulations de Delaunay. Deux types d’approches sont présentés et discutés. Le premier type suit une approche en trois-passes classique: 1) la construction d’un graphe de proximité contenant une information topologique, 2) la construction d’une information statistique à partir de ce graphe et 3) la suppression d’éléments inutiles au regard de cette information statistique. L’impact de différentes measures sur le clustering ainsi que sur la reconnaissance de caractères est discuté. Ces mesures s’appuyent sur l’exploitation du complexe simplicial et non pas uniquement sur celle du graphe. Le second type d’approches est composé d’approches en une passe extrayant des clusters en même temps qu’une triangulation de Delaunay est construiteThis thesis aims at proposing and discussing several solutions to the problem of low-dimensional point cloudanalysis and clustering. These solutions are based on the analysis of the Delaunay triangulation.Two types of approaches are presented and discussed. The first one follows a classical three steps approach:1) the construction of a proximity graph that embeds topological information, 2) the construction of statisticalinformation out of this graph and 3) the removal of pointless elements regarding this information. The impactof different simplicial complex-based measures, i.e. not only based on a graph, is discussed. Evaluation is madeas regards point cloud clustering quality along with handwritten character recognition rates. The second type ofapproaches consists of one-step approaches that derive clustering along with the construction of the triangulation
44
Tasoglu, Ali Ozgur. "Analysis And Design Of Cylindrically Conformal Microstrip Antennas." Master's thesis, METU, 2011. http://etd.lib.metu.edu.tr/upload/12613441/index.pdf.
Full textAbstract:
Cylindrically conformal microstrip antennas are investigated. Two different structures, namely proximity coupled and E-shaped microstrip antennas are analyzed and information about the design parameters is obtained by means of parametric study. With these structures, cylindrical arrays, having omnidirectional radiation in the circumferential plane of the cylinder, are designed. Proximity coupled cylindrical arrays operate in the 2.3-2.4 GHz aeronautical telemetry band with approximately 4% bandwidth. On the other hand, more than 30% bandwidth is obtained by E-Shaped cylindrical array
antenna structure, which also includes the commercial telemetry band. In order to verify the simulation method, a fabricated antenna in literature is simulated and acceptable agreement with simulation and fabrication results obtained.
45
Jarvius, Jonas. "DNA Tools and Microfluidic Systems for Molecular Analysis." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7079.
Full text
46
Jordaan, Jacob A. "Foreign direct investment, externalities and geography : an analysis of the effects of geographic proximity on the externalities from FDI in Mexican manufacturing industries." Thesis, London School of Economics and Political Science (University of London), 2004. http://etheses.lse.ac.uk/2125/.
Full textAbstract:
Contemporary research on externalities from FDI suffers from two central problems. First, estimates of such externalities may be biased when FDI is endogenous to the empirical model. Second, there is an important lack in empirical research regarding the identification of structural factors influencing the type and level of FDI-induced externalities. The goal of the thesis is to address both issues. It starts with a theoretical discussion of FDI and externalities, followed by an overview of contemporary empirical research, highlighting the main estimation problems. Next, theories of agglomeration economies are discussed, in an attempt to identify a determinant of FDI-induced externalities in the form of geographical proximity of manufacturing activities. This discussion, supported by an overview of the limited related available empirical evidence, indicates that this concept is a likely candidate to be such a determinant. The next two chapters use unpublished and thus far unexplored data from the 1993 Mexican economic census to estimate FDI-induced externalities in Mexican manufacturing industries. In this part, the main empirical model is developed and estimated. In addition, the robustness of the initial findings of this empirical model is assessed. Furthermore, the estimation issues that are identified in the first part of the thesis are addressed. Most importantly, I introduce an instrumental variable estimation that controls for the problem of endogenous FDI. This instrumental variable estimation functions satisfactorily; as such, it represents the first successful empirical unbiased estimation of FDI-induced externalities in a cross-sectional setting. Finally, the last part of the thesis offers empirical evidence of the effects of geographical proximity on FDI-induced externalities. The findings indicate that geographical proximity does influence such externalities in a multi-faceted fashion. First, the level of geographical concentration of an industry enhances the occurrence of positive externalities within an industry. Second, from a regional point of view, geographical proximity enhances the occurrence of externalities that arise within and between industries in a region. Third, FDI-induced externalities that arise between industries also appear to spill over between neighbouring regions.
47
Feng, Minggang. "How Will Proximity to Higher Education Affect the rental Housing Market? A Case in Beijing : A Regression Analysis of Rent Level in Beijing." Thesis, KTH, Fastigheter och byggande, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-254741.
Full textAbstract:
Education would always be an important issue in China. Capitalization of education wouldhave impact on housing price and rent price and affect the housing market, this can bereflected from the phenomenon of ‘over-priced school district house’. While thecapitalization of higher education on rent price is always be neglected.In that case, the propose of this study is to estimate the relationship between highereducation and rental market price level in China based on the rental market in Beijing.And the study would focus four zone in Beijing, each of them contains one to twouniversity district and surrounding non-university district.Hedonic pricing model would be used in this study to determine the impact of independentvariables on the rent price development.The result of this study indicates a complex relationship, it finds that the relationshipbetween higher education and rent price in different zones while most zones shows apositive relationship, and the overall result is insignificant. And according to our research,some target suggestions were pointed out.Utbildning är alltid en viktig fråga i Kina. Kapitalisering av utbildningen skulle fåinverkan på bostadspris och hyrespris och påverka bostadsmarknaden, vilket kanåterspeglas från fenomenet "dyrt skolhus". Medan kapitalisering av akademisk utbildningpå hyreskostnaden alltid försummas.I det fallet är förslaget till denna undersökning att uppskatta förhållandet mellan akademiskutbildning och hyresmarknadsprisnivå i Kina baserat på hyresmarknaden i Peking. Ochstudien skulle fokusera på fyra zoner i Peking, var och en av dem innehåller ett till tvåuniversitetsdistrikt och omgivande icke-universitetsdistrikt.Hedoniska prissättningsmodell skulle användas i denna studie för att bestämma effekternaav oberoende variabler på hyresprisutvecklingen.Resultatet av denna studie indikerar ett komplicerat förhållande, det konstaterar attförhållandet mellan akademisk utbildning och hyrespris i olika zoner medan de flesta zonervisar ett positivt förhållande och det övergripande resultatet är obetydligt. Enligt vårforskning pekades några målförslag ut.
48
Schmidt, Jason Knudsen. "Analysis of Square-Root Kalman Filters for Angles-Only Orbital Navigation and the Effects of Sensor Accuracy on State Observability." DigitalCommons@USU, 2010. https://digitalcommons.usu.edu/etd/627.
Full textAbstract:
Angles-only navigation is simple, robust, and well proven in many applications. However, it is sometimes ill-conditioned for orbital rendezvous and proximity operations because, without a direct range measurement, the distance to approaching satellites must be estimated by firing thrusters and observing the change in the target's bearing. Nevertheless, the simplicity of angles-only navigation gives it great appeal. The viability of this technique for relative navigation is examined by building a high-fidelity simulation and evaluating the sensitivity of the system to sensor errors. The relative performances of square-root filtering methods, including Potter, Carlson, and UD factorization filters, are compared to the conventional and Joseph formulations. Filter performance is evaluated during closed-loop "station keeping" operations in simulation.
49
Sinha, Tanay Kumar. "Validation and optimization of multiplexInSitu PLA for signalling pathway analysis." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-450392.
Full textAbstract:
With the advent of Tyrosine kinase inhibitors (TKI) as a therapy for Chronic myeloid Leukemia (CML), the patients now enjoy a life expectancy close to that of the general population. But some patients do get unresponsive to the TKI treatment over time due to several mutations in the kinase domain of the BCR-ABL fusion protein, which further leads to activation of multiple signaling cascades within the leukemic cell, helping it survive and proliferate. This project validates and optimizes a new method of In situ PLA that incorporates the usage of different padlocks and template oligos. Multiple cross-reactivity tests and interaction assays in multiple cancer cell lines will further optimize this system as a robust multiplex protein-protein interaction detection tool. Proteins associated with the MAP-K, PI3-K, and Jak-STAT signaling pathways were the main detection targets.
50
Cheng, Tao. "Automated safety analysis of construction site activities using spatio-temporal data." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/47564.
Full textAbstract:
During the past 10 years, construction was the leading industry of occupational fatalities when compared to other goods producing industries in the US. This is partially attributed to ineffective safety management strategies, specifically lack of automated construction equipment and worker monitoring. Currently, worker safety performance is measured and recorded manually, assessed subjectively, and the resulting performance information is infrequently shared among selected or all project stakeholders. Accurate and emerging remote sensing technology provides critical spatio-temporal data that has the potential to automate and advance the safety monitoring of construction processes.
This doctoral research focuses on pro-active safety utilizing radio-frequency location tracking (Ultra Wideband) and real-time three-dimensional (3D) immersive data visualization technologies. The objective of the research is to create a model that can automatically analyze the spatio-temporal data of the main construction resources (personnel, materials, and equipment), and automatically measure, assess, and visualize worker's safety performance. The research scope is limited to human-equipment interaction in a complex construction site layout where proximities among construction resources are omnipresent. In order to advance the understanding of human-equipment proximity issues, extensive data has been collected in various field trials and from projects with multiple scales. Computational algorithms developed in this research process the data to provide spatio-temporal information that is crucial for construction activity monitoring and analysis. Results indicate that worker's safety performance of selected activities can be automatically and objectively measured using the developed model.
The major contribution of this research is the creation of a proximity hazards assessment model to automatically analyze spatio-temporal data of construction resources, and measure, evaluate, and visualize their safety performance. This research will significantly contribute to transform safety measures in construction industry, as it can determine and communicate automatically safe and unsafe conditions to various project participants located on the field or remotely.