Journal articles on the topic 'Province of Australasia'

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1

Liu, Ke-Ke, Yuan-hao Ying, Alexander A. Fomichev, Dan-chen Zhao, Wen-hui Li, Yong-hong Xiao, and Xiang Xu. "Crab spiders (Araneae, Thomisidae) of Jinggang Mountain National Nature Reserve, Jiangxi Province, China." ZooKeys 1095 (April 13, 2022): 43–74. http://dx.doi.org/10.3897/zookeys.1095.72829.

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A list of 34 thomisid species belonging to 21 genera collected in Jangxi Province of China is provided. Five new species are described: Angaeus xieluae Liu, sp. nov. (♂♀), Lysiteles subspirellus Liu, sp. nov. (♀), Oxytate mucunica Liu, sp. nov. (♀), Pharta lingxiufengica Liu, sp. nov. (♀), Stephanopis xiangzhouica Liu, sp. nov. (♀). A new combination is proposed: Ebelingia forcipata (Song & Zhu, 1993) comb. nov. (ex. Ebrechtella Dahl, 1907). Previously unknown females of E. forcipata (Song & Zhu, 1993), Oxytate bicornis Liu, Liu & Xu, 2017, and Xysticus lesserti Schenkel, 1963 are described for the first time. Stephanopis O Pickard-Cambridge, 1869, a genus previously known from Australasia and South America, is recorded from the Asian mainland for the first time.
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2

Jacob, W. M. "George Augustus Selwyn, First Bishop of New Zealand and the Origins of the Anglican Communion." Journal of Anglican Studies 9, no. 1 (September 14, 2010): 38–55. http://dx.doi.org/10.1017/s1740355310000070.

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AbstractThis article aims to identify the significance of George Augustus Selwyn, the first Bishop of New Zealand, for the development of the Anglican Communion. It is based on evidence derived from secondary sources, most obviously the two-volume life of Selwyn written shortly after his death by his former chaplain, and on recent studies of the development of the Anglican Communion, especially the development of provincial synodical government in Australasia, and on the constitution of the Episcopal Church in the United States.The article concludes that Selwyn had ideal qualities and experiences to enable him to achieve a constitution for a new Anglican province independent of the state, and with self-government, including elected representatives of laity and clergy, as well as bishops meeting together. His commitment to creating a constitutional framework for the dioceses and provinces of the Anglican Communion, including the Episcopal Church, enabled a second Lambeth Conference to happen.
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3

Hendrich, Lars, Helena Shaverdo, Jiří Hájek, and Michael Balke. "Taxonomic revision of Australian Copelatus Erichson, 1832 (Coleoptera, Dytiscidae, Copelatinae)." ZooKeys 889 (November 14, 2019): 81–152. http://dx.doi.org/10.3897/zookeys.889.39090.

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The genus Copelatus in Australia is revised and nine species are recognised. One new species, Copelatus martinbaehrisp. nov., is described from Papua New Guinea (Central Province) and Cape York Peninsula (Iron Range NP and Mt Tozer). Copelatus divisus Watts, 1978, syn. nov., is considered a junior synonym of C. portior Guignot, 1956, described from New Guinea. Species delimitation is based on the morphological characters and Cox1 data. All species are (re)described, and their important species characters (median lobes, parameres, habitus and colour patterns) are illustrated. A key to all nine species is provided. The known distribution and habitat preferences of each species are outlined briefly. In Australia, all nine species are distributed in the northern half of the continent. Four species are also reported from New Guinea: in addition to C. martinbaehrisp. nov., we record C. clarki Sharp, 1882 for the first time from southern New Guinea, and consider literature records of C. irregularis W.J. Macleay, 1871 and C. marginatus Sharp, 1882 from New Guinea as doubtful. Copelatus portior is widely distributed in Australasia, while C. tenebrosus is widely distributed in the Indomalayan and Australasian realms. All Australian Copelatus are confirmed to be lentic, found in a large variety of stagnant water, mainly in lowland areas up to 250 m.
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4

Heinsohn, TE. "Possum extinctions at the marsupial frontier: the status of the northern common cuscus Phalanger orientalis on Santa Ana Island, Makira Province, Solomon Islands." Australian Mammalogy 24, no. 2 (2002): 247. http://dx.doi.org/10.1071/am02247.

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ON zoogeographic maps, the Solomon Islands are shown as the north-eastern limit of Australidelphian marsupial distribution in Australasia. This distinction is due to the presence of a single New Guinean marsupial, the northern common cuscus Phalanger orientalis, which was probably introduced via the Bismarck Archipelago by prehistoric human agency (Flannery 1995; Spriggs 1997; Heinsohn 1998; Wickler 2001). P. orientalis is found across most of the principal Solomon Islands, with the exception of the remote far-eastern oceanic islands of Santa Cruz (Temotu) Province. In the scientific literature, the exact eastern limit of distribution for P. orientalis is generally given as San Cristobal (Makira) Island in Makira Province (Laurie and Hill 1954; Flannery 1995), the eastern most peninsula of which extends to 162� 23' E. The next landmass to the east is the small 5 km diameter and 143 m high limestone atoll of Santa Ana (Owa Rafa) which lies across a 7.5 km open water crossing.
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5

Zinsmeister, William J., and Miguel Griffin. "Late Cretaceous and Tertiary aporrhaid gastropods from the southern rim of the Pacific Ocean." Journal of Paleontology 69, no. 4 (July 1995): 692–702. http://dx.doi.org/10.1017/s0022336000035216.

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The new subfamily Struthiopterinae is proposed for the aporrhaid gastropods occurring in the Late Cretaceous-early Tertiary Weddellian Province along the southern margin of the Pacific. The following genera are placed within the Struthiopterinae: Struthioptera Finlay and Marwick, 1937; Austroaporrhais n. gen.; and Struthiochenopus n. gen. The temporal and biogeographic distribution of members of Struthiopterinae show a similar pattern to other Southern Hemisphere groups of Late Cretaceous and early Cenozoic molluscs with initial disappearance from the western Australasia of the Weddellian Province by the Paleocene while surviving in Antarctica until the late Eocene and eventually disappearing in southern South America during the early Miocene.Also included in this paper is a reappraisal of the species assignable to these genera from Late Cretaceous and early Tertiary of New Zealand, Antarctica, and southern South America together with the description of five new species. The following new species of the Struthiopterinae are described: Austroaporrhais larseni n. sp., A. stilwelli n. sp., A. dorotensis n. sp., Struthiochenopus antarcticus n. sp., and S. philippii n. sp.
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6

Parnaby, Harry E., Peter D. Dwyer, and Kristofer M. Helgen. "Notes on mammals collected on the 1885 Geographical Society of Australasia’s Expedition to New Guinea." Records of the Australian Museum 75, no. 2 (April 26, 2023): 79–86. http://dx.doi.org/10.3853/j.2201-4349.75.2023.1828.

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In 1885 the Geographical Society of Australasia sent the steam launch Bonito to chart the Fly and Strickland Rivers (now in Western Province, Papua New Guinea). The Expedition spent five months in New Guinea with the primary objectives of survey and biological exploration. The type locality of the murid rodent Melomys muscalis froggatti Troughton, 1937, described from a single specimen obtained during the Expedition, is localized to the region of the base camp at Observation Bend, Strickland River, based on the original Expedition maps and the unpublished diary reminiscence of the collector W. W. Froggatt. A review of the mammal collection obtained during that Expedition has not been reported previously, perhaps due to the poor surviving documentation. Several months after the Expedition returned to Sydney, Australian Museum curator E. P. Ramsay prepared a list of 22 mammal specimens received by the Museum. Twenty specimens attributed to the Expedition were not entered into the collection registers until 1913, half of which are likely to be incorrectly associated with the Expedition. Most specimens were registered with very limited data about collection date and locality. Of the 22 specimens originally received by Ramsay, eight rodents and two flying foxes (Pteropus spp.) have not been located in the Collection, but might remain unrecognized as specimens with no data. It is possible that additional mammal specimens were sent by the Geographical Society of Australasia to other institutions at the conclusion of the Expedition.
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7

USTA, Mustafa, Abdullah GÜLLER, and Hikmet Murat SİPAHİOĞLU. "First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye." Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 33, no. 1 (March 31, 2023): 54–63. http://dx.doi.org/10.29133/yyutbd.1205091.

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Phytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, the identification of phytoplasmas infecting tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Five plants, two symptomatic and three asymptomatic, were tested to verify phytoplasma infection. Total DNA isolated from 5 leaf samples was used as a template for PCR reactions. The phytoplasma agents were confirmed in the two symptomatic samples. BLASTn search of 16S rRNA of two sequences shared identity similarity of 99.84% with ‘Candidatus Phytoplasma australasia’. Computer-simulated virtual RFLP profiles show that the 16S rRNA sequences is identical to the reference pattern of the 16SrII-D subgroup, with a similarity coefficient of 1.00. Based on BLAST, virtual RFLP, and phylogenetic dendrogram, the identified phytoplasma strains are enclosed in the 16SrII-D subgroup. This is the first report of tomato witches' broom disease related to 16SrII-D subgroup phytoplasma strains in the Antalya province of Türkiye.
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8

S.P. Singh and P. Rethinam. "Coconut hispine beetle Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae)." CORD 20, no. 01 (December 1, 2004): 34. http://dx.doi.org/10.37833/cord.v20i01.376.

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Coconut hispine beetle, Brontispa longissima (Gestro) was originally described from the Aru Islands (Maluku Province). It is native to Indonesia (Aru Islands, Maluku Province and possibly to Papua Province formerly known as Irian Jaya), and also to Papua New Guinea, including the Bismarck Archipelago, where it seldom causes serious problems. It has now spread widely in Asia, Australasia and Pacific Islands attacking not only coconut palm but also several other cultivated and wild palms. In recent times it has spread to Singapore, Vietnam, Nauru, Thailand, Maldives and Hainan Island (China). In the absence of natural antagonists it has become a very serious and devastating pest in new areas of its spread. It is feared that B. longissima will find its way from Maldives to Sri Lanka and southern parts of India to derail the economy of these important coconut-growing regions of the world. Thus emergency operations are necessary to try to decimate it down in the Maldives. A number of natural enemies such as Hispidophila (Haeckeliania) brontispae Ferriere, Ooncyrtus podontiae Gahan, Trichogrammatoidea nana Zehntner, Tetrastichus brontispae Ferriere, Asecodes hispinarum Boucek, Chrysonotomyia sp., Metarhizium anisopliae (Metchnikoff) Sorokin, Chelisoches morio Fabricius, Oecophylla smaragdina (Fabricious), mites on adults (Anoplocelaeno sp. and Celaenopsis sp.), geckoes, skinks, tree frog and unidentified bacterial pathogen have been recorded. Biological control by introduction and enhancement of parasitoids- A. hispinarum and T. brontispae has proved very effective. Similarly spray of improved strains of entomopathogenic fungus, M. anisopliae has proved effective. Exploratory surveys for parasitoids in the original home of B. longissima are suggested.
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9

Li, Tian, Chuankuo Zhao, Yuxin Guo, Jinze Dong, Fanshu Du, Yong Zhou, Sicheng Shu, et al. "Genetic and Biological Characteristics of Duck-Origin H4N6 Avian Influenza Virus Isolated in China in 2022." Viruses 16, no. 2 (January 30, 2024): 207. http://dx.doi.org/10.3390/v16020207.

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The interaction between migratory birds and domestic waterfowl facilitates viral co-infections, leading to viral reassortment and the emergence of novel viruses. In 2022, samples were collected from duck farms around Poyang Lake in Jiangxi Province, China, which is located within the East Asia–Australasia flyway. Three strains of H4N6 avian influenza virus (AIV) were isolated. Genetic and phylogenetic analyses showed that the isolated H4N6 avian influenza viruses (AIVs) belonged to new genotypes, G23 and G24. All isolated strains demonstrated dual receptor binding properties. Additionally, the isolated strains were able to replicate efficiently not only in avian cells but also in mammalian cells. Furthermore, the H4N6 AIV isolates could infect chickens, with viral replication detected in the lungs and extrapulmonary organs, and could transmit within chicken flocks through contact, with viral shedding detected only in oropharyngeal swabs from chickens in the contact group. Notably, the H4N6 AIV could infect mice without prior adaptation and replicate in the lungs with high viral titers, suggesting that it is a potential threat to humans. In conclusion, this study provides valuable insight into the characteristics of H4N6 strains currently circulating in China.
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10

Xu, Chen, Ni Yu-Nan, Charles E. Mitchell, Qiao Xin-Dong, and Zhan Shi-Gao. "Graptolites from the Qilang and Yingan formations (Caradoc, Ordovician) of Kalpin, western Tarim, Xinjiang, China." Journal of Paleontology 74, no. 2 (March 2000): 282–300. http://dx.doi.org/10.1017/s0022336000031498.

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Late Ordovician rocks of the Qilang and Yingan formations from the Kalpin area in the Tarim region of western Xinjiang, China (Tarim palaeoplate) contain a moderately diverse graptolite fauna. The fauna from the Qilang Formation containsCorynoides calicularisNicholson, 1867;Dicranograptus clingani resicisWilliams and Bruton, 1983;Lasiograptus costatusLapworth, 1873;Pseudoclimacograptus scharenbergi(Lapworth, 1876); andGlossograptussp. among other species. This assemblage most likely corresponds to the upperClimacograptus (Climacograptus) bicornisZone? to lowerC. (Diplacanthograptus) lanceolatusZone of Australia. The Qilang Formation also yields the new taxon,Amplexograptus maxwelli spinoususnew subspecies. The overlying Yingan Formation yields a more diverse assemblage that includesClimacograptus (Diplacanthograptus) spiniferusRuedemann, 1912;C. (D.) lanceolatusVandenBerg, 1990;Orthograptus quadrimucronatus(Hall, 1865);Amplexograptus praetypicalisRiva, 1987;Dicellograptus pumilusLapworth, 1876; andD. morrisiHopkinson, 1871. This assemblage most likely corresponds to theCorynoides americanusZone through theC. (D.) spiniferusZone of eastern Laurentia, or to theD. clinganiZone of Scotland and central Newfoundland. The presence of aC. (D.) lanceolatusandC. (D.) spiniferussuccession in the Yingan Formation also suggests correlation with the Eastonian 1 and 2 of Australasia. The Yingan Formation faunas represent an offshore Pacific Province assemblage dominated by cosmopolitan epipelagic species. The fauna is most similar, both in terms of species composition and relative abundance patterns, to those of the Appalachian Basin. The Yingan faunas differ from the latter in the absence of Laurentian endemic species (except forAmplexograptus praetypicalis), and in the common occurrence of dicellograptids.
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Georges, Arthur, Fiorenzo Guarino, and Biatus Bito. "Freshwater turtles of the TransFly region of Papua New Guinea - notes on diversity, distribution, reproduction, harvest and trade." Wildlife Research 33, no. 5 (2006): 373. http://dx.doi.org/10.1071/wr05087.

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The Chelidae is a family of side-necked turtles restricted in distribution to South America and Australasia. While their biology in Australia is reasonably well known, species in New Guinea are very poorly known despite high diversity, especially in the southern lowlands. In this paper, we report on the diversity, distribution, habitat and reproductive biology of the freshwater turtles of the TransFly region of the Western Province of Papua New Guinea, with special emphasis on reproduction of Emydura subglobosa, Elseya branderhorsti and Elseya novaeguineae. Seven species were captured, with reliable records of an eighth. A key to the freshwater turtles of the TransFly region is provided. Harvest methods, consumption, and trade in turtles by the TransFly communities are documented. There is mounting pressure to take advantage of revenue opportunities afforded by the Asian turtle trade, but this is impeded by lack of transport infrastructure. There is also insufficient demographic information on any New Guinean turtle species to make a reasoned judgment on the level of harvest that would be sustainable. Nor is there sufficient information on captive rearing for most species, and where it is available it is not accessible by local villagers. These knowledge gaps need to be addressed and factored into a management plan that is implemented before local communities can capitalise on the commercial opportunities provided by the turtle fauna without risking collapse of the resource and the implications for their concurrent subsistence economy that would follow.
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GÉNIER, FRANÇOIS, and ADRIAN L. V. DAVIS. "Digitonthophagus gazella auctorum: an unfortunate case of mistaken identity for a widely introduced species (Coleoptera: Scarabaeidae: Scarabaeinae: Onthophagini)." Zootaxa 4221, no. 4 (January 19, 2017): 497. http://dx.doi.org/10.11646/zootaxa.4221.4.8.

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At risk of committing entomological heresy, we question the identity of a dung-burying beetle species that originates from Africa and has been introduced first into Hawaii and subsequently to Australasia, North America, and South America (Fincher 1986; Edwards 2007; Noriega et al. 2010) for pasture improvement and biological control of dung-breeding flies (Waterhouse 1974; Bornemissza 1979). Under the name Onthophagus gazella (Fabricius 1787), it was the first species selected for introduction into Australia by the CSIRO Dung Beetle Project (Bornemissza 1976; Edwards 2007). Firstly, in 1968, a "tropical strain" was introduced from Hawaii where it had become established after introduction from Zimbabwe in 1957 (Markin & Yoshioka 1998). Later, after establishment of the CSIRO Dung Beetle Research Unit in Pretoria in 1970, a "cold" or "even rainfall strain" was introduced into Australia directly from South Africa (Bornemissza 1976) (even rainfall region = south coast of Eastern Cape). The species was subsequently introduced into the southern continental United States of America (Victoria County, Texas) from Hawaii (Montes de Oca & Halffter 1998) then elsewhere into southeastern and southwestern states from Hawaii and breeding colonies from Australia (Anderson & Loomis 1978). It has since expanded its range through Mexico, Central America, and the Caribbean to coastal Colombia (Kohlmann 1994; Noriega 2002; Noriega et al. 2006, 2011). Expansion of its range within central southern South America (Noriega et al. 2010) has been assisted by introductions into Brazil from the United States of America since the 1980s (Bianchin et al. 1998), and others into Venezuela and Chile (Vidaurre et al. 2008). More recently, it has been introduced into quarantine and field trials in New Zealand (Forgie et al. 2013) using individuals originating from the south coast of the Eastern Cape and Northwest Province of South Africa (S. Forgie, personal communication).
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LOKTIONOV, VALERY M. "A review of the spider wasp genus Austrosalius Turner, 1917 (Hymenoptera, Pompilidae), with the description of a new species." Zootaxa 5006, no. 1 (July 29, 2021): 121–26. http://dx.doi.org/10.11646/zootaxa.5006.1.16.

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The Australasian spider wasp genus Austrosalius Turner, 1917 is reviewed for the first time. The genus comprises three species, of them Austrosalius mikhailovi Loktionov, sp. nov. is described and illustrated, based on female specimens from West Papua Province of Indonesia. The genus Austrosalius is newly reported from the island of New Guinea. The generic diagnosis and a key to the species are given.
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Klymko, John, Paul Catling, Jeffrey B. Ogden, Robert W. Harding, Donald F. McAlpine, Sarah L. Robinson, Denis A. Doucet, and Christoper I. G. Adam. "Orthoptera and allies in the Maritime provinces, Canada: new records and updated provincial checklists." Canadian Field-Naturalist 132, no. 4 (July 11, 2019): 319–29. http://dx.doi.org/10.22621/cfn.v132i4.1984.

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We provide an updated checklist of Orthoptera and their allies for each Maritime province of Canada with details for 21 new species records. Drumming Katydid (Meconema thalassinum), recorded from Nova Scotia (NS) and Prince Edward Island (PEI), and Sprinkled Grasshopper (Chloealtis conspersa), recorded from New Brunswick (NB) are reported for the first time from the Maritimes as a whole. We report range extensions in the Maritime region for Australian Cockroach (Periplaneta australasiae; NB), Treetop Bush Katydid (Scudderia fasciata; NS), Short-legged Camel Cricket (Ceuthophilus brevipes; PEI), Spotted Camel Cricket (Ceuthophilus maculatus; PEI), Roesel’s Shield-backed Katydid (Roeseliana roesellii; NS), and Black-horned Tree Cricket (Oecanthus nigricornis; PEI). Short-winged Mole Cricket (Neoscapteriscus abbreviatus; NB) and European Mole Cricket (Gryllotalpa gryllotalpa; NS) are reported as adventives (non-native species that are believed to be not yet established), new to Canada from the Maritimes. Other new records for species not known to be established are Lined Earwig (Doru taeniatum; NS), Australian Cockroach (Periplaneta australasiae; PEI), American Cockroach (Periplaneta americana; NB), Brown Cockroach (Periplaneta brunnea; PEI), Smooth Cockroach (Nyctibora laevigata; NB), West Indian Leaf Cockroach (Blaberus discoidalis; NB), an unidentified Parcoblatta species (NB), Brown-banded Cockroach (Supella longipalpa; PEI), Praying Mantis (Mantis religiosa; NB), and American Bird Grasshopper (Schistocerca americana; NS).
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SCHWEIZER, MANUEL, MARCEL GÜNTERT, and STEFAN T. HERTWIG. "Out of the Bassian province: historical biogeography of the Australasian platycercine parrots (Aves, Psittaciformes)." Zoologica Scripta 42, no. 1 (September 3, 2012): 13–27. http://dx.doi.org/10.1111/j.1463-6409.2012.00561.x.

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16

Lyons, Jessica Ann, Daniel James Deans Natusch, and Chris R. Shepherd. "The harvest of freshwater turtles (Chelidae) from Papua, Indonesia, for the international pet trade." Oryx 47, no. 2 (April 2013): 298–302. http://dx.doi.org/10.1017/s0030605312000932.

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AbstractThe international trade in wildlife is currently one of the greatest threats to biodiversity. One group, the turtles, has experienced far-reaching population declines and extinctions because the majority of animals are sourced from the wild. The island of New Guinea has the highest diversity of freshwater turtles (Chelidae) in the Australasian region and large numbers are harvested from the Indonesian province of Papua to supply the international pet trade. A total of 264 of these turtles representing six species were recorded between December 2010 and March 2011 while gathering information about wildlife trade in the Indonesian province of Papua. Most were juveniles, although a substantial number of large adults were also harvested. None of the species recorded are CITES-listed. Despite one species not being allocated an annual harvest quota, it was observed being traded. Illegal and unregulated trade coupled with a lack of basic ecological data for these species can have a severe impact on wild populations. We present recommendations for law enforcement and conservation of these species.
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Phuong-Thao, Nu Nguyen, Tran Truc-Xuan, Pham Thi-Hoa, and Nguyen Kim-Dung. "Livelihoods and human impacts in Tan Thanh mudflat, Tien Giang Province, Vietnam." Research Journal of Biotechnology 18, no. 8 (July 15, 2023): 46–53. http://dx.doi.org/10.25303/1808rjbt046053.

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Tan Thanh mudflat in Tien Giang Province is one of the important wetlands of the Mekong Delta in Southern Vietnam. International conservation organizations have proposed this area to be an important bird and biodiversity area – IBAs because of the near location to the core of the East Asian-Australasian Flyway of migratory birds and providing feeding habitats for local shorebirds and endangered migratory species such as the Spoon-billed Sandpiper (Calidris pygmaea). To understand the threats human beings have on this proposed protected wetland, we conducted a study in August 2022 to assess the impacts of local community activities on the area. We used structured interviews with a site survey and mapping as the main methods of the study. The result showed that anthropogenic causes including agrochemical overuse, solid waste pollution, frequent disturbances from clam harvesting activities and illegal wild bird catching to protect cultivated farms, caused adverse impacts on the area. The study also revealed that the local communities have not been fully aware of the responsibility of protecting wild birds and the coastal environment. We suggested that to conserve the wetland successfully, we need to do further studies to fully understand the values of the wetland services to the wild birds and local communities and then engage relevant stakeholders to find solutions for improving public awareness about the importance of the wetland and inclusively protecting the area for sustainable development.
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Liu, Yuan, Xianglin Ji, and Lizhi Zhou. "Assessment of Waterbird Habitat Importance and Identification of Conservation Gaps in Anhui Province." Animals 14, no. 7 (March 25, 2024): 1004. http://dx.doi.org/10.3390/ani14071004.

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Wetlands are among the most important habitats of highly wetland-dependent waterbirds but are subject to ongoing habitat loss and degradation owing to intensified anthropogenic activities. The scarcity of human and natural resources makes effective habitat protection an important concern. Here, we aimed to investigate waterbird habitat protection methods for Anhui Province, China, a critical stopover and wintering area on the East Asian-Australasian Flyway that features rich wetland resources subject to significant habitat loss and degradation. We evaluated the status and importance of 306 wintering waterbird habitats and identified the key environmental influences and current protection gaps using the entropy weights method and generalized additive modeling. We found 73 important habitats for waterbirds in Anhui Province, which were classified into levels of importance (descending from I to V) according to the natural discontinuity method. Level I and Level II habitats were mainly located in the Yangtze River floodplain and Level IV habitats in the Huaihe River floodplain. The gap analysis showed that 42 important waterbird habitats had protection gaps, accounting for 57.53% of the total area. Waterbird habitat importance was significantly correlated with elevation, normalized vegetation index, lake area, and lake circumference but not with distance from roads or population density. The results of this study provide scientific information for waterbird habitat conservation planning, which is crucial for maintaining wetland ecosystem functions.
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Ewart, A., R. W. Schon, and B. W. Chappell. "The Cretaceous volcanic-plutonic province of the central Queensland (Australia) coast—a rift related ‘calc-alkaline’ province." Earth and Environmental Science Transactions of the Royal Society of Edinburgh 83, no. 1-2 (1992): 327–45. http://dx.doi.org/10.1017/s0263593300008002.

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ABSTRACTSilicic and minor intermediate and mafic pyroclastics, lavas, and dykes occupy a NW-trending zone through the Whitsunday, Cumberland and Northumberland Island groups, and locally areas on the adjacent mainland, over a distance of more than 300 km along the central Queensland coast. K-Ar and Rb-Sr data indicate an age range of 95–132 Ma, with the main activity approximately between 105–120 Ma; there is, however, evidence for easterly increasing ages. Comagmatic granites, some clearly intrusive into the volcanics, occur together with two localised areas of Triassic potassic granites (229 Ma), that form the immediate basement.The volcanics are dominantly rhyolitic to dacitic lithic ignimbrites, with intercalated surge and bedded tuffs, accretionary lapilli tuffs, and lag deposits. Associated rock types include isolated rhyolitic and dacitic domes, and volumetrically minor andesite and rare basalt flows. The sequence is cut by abundant dykes, especially in the northern region and adjacent mainland, ranging from dolerite through andesite, dacite and rhyolite. Dyke orientations show maxima between NW-NNE. Isotope data, similarities in petrography and mineralogy, and alteration patterns all suggest dyke intrusion to be broadly contemporaneous with volcanism. The thickness of the volcanics is unconstrained, although in the Whitsunday area, minimum thicknesses of >1 km are inferred. Eruptive centres are believed to occur throughout the region, and include at least two areas of caldera-style collapse. The sequences are thus considered as predominantly intracaldera.The phenocryst mineralogy is similar to modern “orogenic” volcanics. Phases include plagioclase, augite, hypersthene (uralitised), magnetite, ilmenite, with less common hornblende, and even rarer quartz, sanidine, and biotite. Fe-enriched compositions only develop in some high-silica rhyolites. The granites range from quartz diorite to granite s.s., and some contain spectacular concentrations of partially disaggregated dioritic inclusions.Chemically, the suite ranges continuously from basalt to high-silica rhyolite, with calc-alkali to high-K affinities, and geochemical signatures similar to modern subduction-related magmas. Only the high-silica rhyolites and granites exhibit evidence of extensive fractional crystallisation (e.g. pronounced Eu anomalies). Variation within the suite can only satisfactorily be modelled in terms of two component mixing, with superimposed crystal fractionation. Nd and Sr isotope compositions are relatively coherent, with εNd + 2·2 to +7·3, and ISr (calculated at 110 and 115 Ma) 0·7031-0·7044. These are relatively primitive, and imply mantle and/or newly accreted crustal magma sources.The two end-members proposed are within-plate tholeiitic melt, and ?low-silica rhyolitic melts generated by partial fusion of Permian (to ?Carboniferous) arc and arc basement. The arc-like geochemistry is thus considered to be source inherited. The tectonic setting for Cretaceous volcanism is correlated with updoming and basin rifting during the early stages of continental breakup, culminating in the opening of the Tasman Basin. Cretaceous volcanism is also recognised in the Maryborough Basin (S Queensland), the Lord Howe Rise, and New Caledonia, indicating the regional extent of volcanism associated with the complex breakup of the eastern Australasian continent margin.
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WILLIAMS, DAVID J., MARK O'SHEA, ROLAND L. DAGUERRE, CATHARINE E. POOK, WOLFGANG WÜSTER, CHRISTOPHER J. HAYDEN, JOHN D. MCVAY, et al. "Origin of the eastern brownsnake, Pseudonaja textilis (Dumeril, Bibron and Dumeril) (Serpentes: Elapidae: Hydrophiinae) in New Guinea: evidence of multiple dispersals from Australia, and comments on the status of Pseudonaja textilis pughi Hoser 2003." Zootaxa 1703, no. 1 (February 13, 2008): 47. http://dx.doi.org/10.11646/zootaxa.1703.1.3.

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Pseudonaja textilis is a widespread and common snake in eastern parts of Australia, but its distribution in New Guinea is poorly understood, and the origin of the New Guinea populations and its timing have been the subject of much speculation. Phylogenetic analysis of mitochondrial DNA sequences from three New Guinea populations of P. textilis indicates that New Guinea was colonised from two independent eastern and western migration routes most likely in the Pleistocene. One dispersal event from northern Queensland led to the populations in eastern New Guinea (Milne Bay, Oro and Central Provinces, Papua New Guinea), whereas another, from Arnhem Land to central southern New Guinea, led to the populations from the Merauke area, Indonesian Papua. The results are consistent with the effects of Pleistocene sea level changes on the physical geography of Australasia, and are thus suggestive of a natural rather than anthropogenic origin of the New Guinea populations. The taxonomic status of the New Guinean populations is discussed.
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Esmaeilzadeh-Hosseini, Seyyed Alireza, Ghobad Babaei, Francesco Pacini, and Assunta Bertaccini. "Multilocus Gene Analyses Indicate Tamarix aphylla as Reservoir Host of Diverse Phytoplasmas Associated with Witches’ Broom and Yellowing Symptomatology." Plants 13, no. 9 (April 30, 2024): 1248. http://dx.doi.org/10.3390/plants13091248.

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Tamarisk witches’ broom, yellowing, and little leaf symptoms were observed during 2018–2023 surveys of rural deserts in central regions of Iran with the highest disease incidence up to 72% in Chah Afzal (Yazd province). A verification of the presence and identity of phytoplasmas associated with these symptoms was then performed. Tamarisk tree branch cuttings obtained from symptomatic plants sprouted up to 90.3% but with 15–25 days’ delay compared to the asymptomatic ones and showed internode shortening and witches’ broom, while the branch cuttings from asymptomatic plants had normal growth and sprouted up to 97.8%. Phytoplasma transmission by dodder bridges to periwinkle did not succeed, while nested polymerase chain reaction on the phytoplasma ribosomal gene followed by RFLP and phylogenetic analyses revealed the presence of ‘Candidatus Phytoplasma asteris’, ‘Ca. P. australasiae=australasiaticum’, and ‘Ca. P. trifolii’ (ribosomal subgroups 16SrI-B, 16SrII-D, and 16SrVI-A, respectively) in the samples from symptomatic plants only. Further amplifications were performed on selected phytoplasma-positive samples on tuf and secA genes, and the produced sequences indicated the presence of mixed phytoplasma infection in some of the samples. In particular, in the tuf gene, a mixed infection of ‘Ca. P. australasiae=australasiaticum’ and ‘Ca. P. trifolii’ was detected, while in the secA gene, the presence of ‘Ca. P. asteris’ or ‘Ca. P. tritici’ strains was identified. The first-time detection of diverse phytoplasma strains in symptomatic T. aphylla suggests that this species represent a relevant source of infection for the agricultural crops and for landscape plants especially when temperature allows insect vector transmission, and therefore, it represents a risk in every environment especially in the frame of climatic changes.
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Hooper, JNA, and C. Levi. "Axinellida (Porifera : Demospongiae) from the New Caledonia lagoon." Invertebrate Systematics 7, no. 6 (1993): 1395. http://dx.doi.org/10.1071/it9931395.

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Sixteen species of axinellid demosponges, including seven new species and seven new locality records, are described from the shallow-water New Caledonian lagoon and reefs [families Axinellidae (12 species, Cyrnbastela, Reniochalina, Axinella, Phakellia, Stylissa, Ptilocaulis, Pseudaxinella, Rhaphoxya) and Desmoxyidae (4 species, Myrrnekioderrna, Higginsia)], bringing the total number of described axinellid species in this region to 25. Brief revisions are provided for several of these genera, based primarily on the Indo-west Pacific fauna, in order to place these New Caledonian species. Non-endemic New Caledonian axinellids belong predominantly to the north-eastern Australian (Solanderian province) and Indo-Malay fauna, usually representing the easternmost extent of these species' distributions in the Indo-west Pacific. Two species, Axinella carteri (Dendy) and Astrosclera willeyana Lister, were found to be truly widely distributed throughout the Indo-Pacific, typically associated with coral reefs; other previously suspected widely distributed species were generally found to be allopatric, cryptic sibling species related to the tropical Australasian fauna.
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23

Eesa A Fredericks. "Contractual Capacity and the Conflict of Laws in Common-Law Jurisdictions (Part 2): Australasia, North America, Asia and Africa." Obiter 41, no. 1 (April 1, 2020): 10–44. http://dx.doi.org/10.17159/obiter.v41i1.10546.

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This series of two articles provides a comparative overview of the position in common-law jurisdictions on the conflict of laws in respect of the contractual capacity of natural persons. The comparative study is undertaken in order to provide guidelines for the future development of South African private international law. Reference is primarily made to case law and the opinions of academic authors. The legal position in the law of the United Kingdom, as the mother jurisdiction in Europe, was investigated in part 1.1 Although Scotland is a mixed civil/common-law jurisdiction, the situation in that part of the United Kingdom was also discussed.Part 2 deals with the rules and principles of private international law in respect of contractual capacity in Australasia (Australia and New Zealand), North America (the common-law provinces of Canada and the United States of America), Asia (India, Malaysia and Singapore) and Africa (Ghana and Nigeria). This part also contains a comprehensive summary of the legal position in the common-law countries, followed by ideas for the reform of South African private international law in this regard.
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24

Tolmacheva, T. Yu, K. E. Degtyarev, and K. N. Shatagin. "Middle ordovician conodonts from the Chingiz Ridge (Kazakhstan): taxonomy of the Naiman Formation assemblage and its biogeographic affinity." Стратиграфия 27, no. 1 (March 4, 2019): 12–30. http://dx.doi.org/10.31857/s0869-592x27112-30.

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Conodonts from carbonate rocks of the Naiman Formation (Chingiz Ridge, East Kazakhstan) have been studied in detail for the first time. The mixed conodont complex is characterized by a high taxonomic diversity and includes species of the stratigraphic interval from the upper part of the Dapingian to the middle of the Darriwilian of the Middle Ordovician. The conodont assemblage includes oceanic/deep-sea species of wide geographic distribution, in particular, abundant Periodon macrodentatus and P. zgierzensis, which are typical for siliceous deposits of Kazakhstan. For the first time, the occurrence of species Scolopodus? mufushanensis, Drepanoistodus latus and Anodontus longus is noted in Kazakhstan. This indicates the existence of the biogeographic climatic zones typical for pelagic biota. The species Protopanderodus? nogamii indicates that the Kazakhstan conodont fauna belongs to the Australasian biogeographic province of the Eastern Gondwana. The conodont assemblage includes a number of regionally endemic taxa that characterize the Kazakhstan fauna as an independent biogeographic unit and confirm the relative remoteness of water basins of Kazakhstan from the eastern margin of Gondwana as well as from other paleocontinents in the Ordovician.
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ZENG, ZHI-YU, JIA WANG, RONALD SLUYS, ZHI-PENG GUO, TING SUN, XIA-ZI HUANG, SHUANG-FEI LI, and AN-TAI WANG. "Integrative description of a new species of Dugesia (Platyhelminthes, Tricladida, Dugesiidae) from Shennongjia, Central China." Zootaxa 5406, no. 4 (February 8, 2024): 535–50. http://dx.doi.org/10.11646/zootaxa.5406.4.3.

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A new species of the genus Dugesia (Platyhelminthes, Tricladida, Dugesiidae) from Xiangxi River, Shennongjia Forestry District, Hubei Province, China, is described on the basis of an integrative approach, involving morphology, and molecular systematics. The new species Dugesia saccaria A-T. Wang & Sluys, sp. nov. is characterized by the following features: a dumb-bell-shaped, muscularized hump located just anterior to the knee-shaped bend in the bursal canal; a ventrally displaced ejaculatory duct, which, however, opens terminally through the dorsal portion of the blunt tip of the penis papilla; a ventrally located seminal vesicle, giving rise to a vertically running duct that eventually curves downwards to communicate with the ejaculatory duct via a small diaphragm; oviducts opening asymmetrically into the dorsal portion of the common atrium and at the knee-shaped part of the bursal canal. The phylogenetic position of the new species was determined using four molecular markers (18S rDNA; ITS-1; 28S rDNA; COI), which suggested that it groups with other species of Dugesia from the Australasian and Oriental biogeographical regions.
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26

Harvey, Mark S., Andrew D. Austin, and Mark Adams. "The systematics and biology of the spider genus Nephila (Araneae:Nephilidae) in the Australasian region." Invertebrate Systematics 21, no. 5 (2007): 407. http://dx.doi.org/10.1071/is05016.

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Five species of the nephilid genus Nephila Leach are found in the Australasian region, which for the purposes of this study was defined as Australia and its dependencies (including Lord Howe I., Norfolk I., Christmas I., Cocos (Keeling) Is), New Guinea (including Papua New Guinea and the Indonesian province of West Papua), Solomon Is, Vanuatu, New Caledonia, Fiji, Tonga, Niue, New Zealand and other parts of the south-west Pacific region. All species are redescribed and illustrated. Nephila pilipes (Fabricius) occurs in the closed forests of eastern and northern Australia, New Guinea, Solomon Is and Vanuatu (through to South-East Asia); N. plumipes (Latreille) is found in Australia (including Lord Howe I. and Norfolk I.), New Guinea, Vanuatu, Solomon Is and New Caledonia; N. tetragnathoides (Walckenaer) inhabits Fiji, Tonga and Niue; N. antipodiana (Walckenaer) occurs in northern Australia (as well as Christmas I.), New Guinea and Solomon Is (through to South-East Asia); and N. edulis (Labillardière) is found in Australia (including Cocos (Keeling) Is), New Guinea, New Zealand and New Caledonia. Epeira (Nephila) walckenaeri Doleschall, E. (N.) hasseltii Doleschall, N. maculata var. annulipes Thorell, N. maculata jalorensis Simon, N. maculata var. novae-guineae Strand, N. pictithorax Kulczyński, N. maculata var. flavornata Merian, N. pictithorax Kulczyński, N. maculata var. flavornata Merian, N. maculata piscatorum de Vis, and N. (N.) maculata var. lauterbachi Dahl are proposed as new synonyms of N. pilipes. Nephila imperialis var. novaemecklenburgiae Strand, N. ambigua Kulczyński, N. sarasinorum Merian and N. celebesiana Strand are proposed as new synonyms of N. antipodiana. Meta aerea Hogg, N. meridionalis Hogg, N. adelaidensis Hogg and N. meridionalis hermitis Hogg are proposed as new synonyms of N. edulis. Nephila picta Rainbow is removed from the synonymy of N. plumipes and treated as a synonym of N. edulis, and N. nigritarsis insulicola Pocock is removed from the synonymy of N. plumipes and treated as a synonym of N. antipodiana. Allozyme data demonstrate that N. pilipes is distinct at the 80% FD level from N. edulis, N. plumipes and N. tetragnathoides. Nephila plumipes and N. tetragnathoides, deemed to represent sister-taxa owing to the shared presence of a triangular protrusion of the male pedipalpal conductor, were found to differ at 15% FD in the genetic study. No genetic differentiation was found between 10 populations of N. edulis sampled across mainland Australia. Species of the genus Nephila have been extensively used in ecological and behavioural studies, and the biology of Nephila species in the Australasian region is extensively reviewed and compared with studies on Nephila species from other regions of the world.
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Tang, Siqi, Bing Han, Chaofan Su, Hailing Li, Shiyuchen Zhao, Haoyu Leng, Yali Feng, and Ying Zhang. "Wild Bird-Origin H6N2 Influenza Virus Acquires Enhanced Pathogenicity after Single Passage in Mice." Viruses 16, no. 3 (February 25, 2024): 357. http://dx.doi.org/10.3390/v16030357.

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The H6 subtype of avian influenza viruses (AIVs) has emerged as one of the predominant subtypes in both wild and domestic avian species. Currently, H6 AIVs have acquired the ability to infect a wide range of mammals, though the related molecular mechanisms have yet to be fully investigated. In this study, a wild bird-origin H6N2 AIV was isolated from the East Asian–Australasian migratory flyway region located in Liaoning Province. This H6N2 virus initially expressed limited replication in mice. However, after one passage in mice, the virus acquired two mutations, PB2 E627K and HA A110V. The mutant displayed enhanced replication both in vitro and in vivo, proving lethal to mice. But the mutant retained the α-2, 3-linked sialic acid binding property and failed to transmit in guinea pigs. We explored the molecular mechanisms underlying the pathogenicity difference between the wild type and the mutant. Our findings revealed that PB2 E627K dramatically enhanced the polymerase activity of the H6N2 virus, while the HA A110V mutation decreased the pH of HA activation. This study demonstrated that the H6N2 subtype wild bird-origin AIV easily acquired the mammalian adaptation. The monitoring and evaluation of H6 wild bird-origin AIV should be strengthened.
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Wijaya, I. Made Saka, and Purnomo Purnomo. "List of Orchid Species along the Northern Trekking Route of Mount Binaiya, Seram Island, Maluku - Indonesia." International Journal of Environment and Geosciences 5, no. 1 (March 12, 2024): 23. http://dx.doi.org/10.24843/ijeg.2024.v05.i01.p03.

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Orchidaceae is widely distributed on Earth with major distribution in the tropical regions, including Indonesia. Seram Island located in Maluku Province Indonesia has a high potential to become a habitat for various species of Australasian orchids. Orchid diversity tends to be related to altitude variations in the area that indirectly contribute to creating microclimate variations. One area on Seram Island with various variations in altitude is Mount Binaiya within the Manusela National Park area. This study aims to identify orchid species found on Mount Binaiya. The exploration method was used on the northern trekking route of Mount Binaiya, divided into Waisamata, Kanikeh, Waiansela, and Waihuhu areas. Based on the result, 47 species of orchids were obtained, which belong to the subfamilies Epidendroideae (35 species), Orchidoideae (11 species), and Vanilloideae (1 species). Based on the life form, 25 species were epiphytes, while 22 species were terrestrial orchids. Waisamata had the highest number of species (23 species), followed by Waiansela (14 species), Waihuhu (14 species), and Kanikeh (8 species). Of all these species, some that need further research are Corybas spp., Cyrtosia nana, Pterostylis papuana, Glomera papuana, and Mediocalcar pygmaeum. Some species are new records in their distribution or rediscoveries of existing records.
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29

Hamli, Hadi, and Abdulla Al Asif. "Trends of bivalve (Bivalvia) research in East Malaysia: a systematic review." Asian-Australasian Journal of Bioscience and Biotechnology 6, no. 2 (August 31, 2021): 50–59. http://dx.doi.org/10.3329/aajbb.v6i2.56140.

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The unique biodiversity in Malaysia makes this country a global hotspot for different aspects of research. Many European and non-native research activities are conducting by different research groups, whereas local institutions also collaborate with them to explore the pristine nature of west (peninsular Malaysia) and East Malaysia (Sarawak and Sabah province). East Malaysia is located in Borneo Island, and the biodiversity of this area are huge. The current review work scrutinized the information about the research activities on aquatic bivalve species and their different aspects of the investigation. This investigation revealed, to date 28 research publications were published on aquatic bivalves from eastern Malaysia, where biodiversity, conservation and ecology was the major aspect of research. The other aspects were aquaculture, natural history and taxonomy, nutritional study, reproduction of bivalve, morphology, and pollution can be mentionable. The major portion of this eastern Malaysia are not investigated and it is assumed that many species are still not reported. Further studies demand to explore the vast bivalve biodiversity of this part of Malaysia. Asian Australas. J. Biosci. Biotechnol. 2021, 6 (2), 50-59
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Setiawan, Agus, Wisnu Nurcahyo, Dwi Priyowidodo, Rina Tri Budiati, and Desy Sylvia Ratna Susanti. "Genetic and parasitological identification of Trypanosoma evansi infecting cattle in South Sulawesi, Indonesia." January-2021 14, no. 1 (2021): 113–19. http://dx.doi.org/10.14202/vetworld.2021.113-119.

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Background and Aim: Sulawesi is an Indonesian island located within the Wallacea region that contains a distinctive mix of Asian and Australasian species. This distinctiveness extends to parasites, including Trypanosoma evansi, the cause of surra. Surra has non-specific clinical signs such as anemia, anorexia, weight loss, drop in milk production, and reproductive disorders which cause economic losses. Due to the trade of livestock, surra has spread in Indonesia from one island to another. The aim of this study was to investigate the trypanosomes infecting cattle in South Sulawesi, using internal transcribed spacer (ITS2) ribosomal DNA (rDNA) sequencing. Materials and Methods: A total of 100 whole blood samples were collected from cattle in Makassar, South Sulawesi Province, Indonesia. All samples were tested using conventional parasitological methods (CPT), namely, thin blood smear, buffy coat smears, and polymerase chain reaction (PCR) testing. Positive PCR results were sequenced and phylogenetically analyzed. Results: Only one of the 100 samples was found to be positive with microscopic observation; however, PCR analysis revealed that 3% (3/100) of samples were positive. Sequencing identified the positive samples as T. evansi, China isolate (KU552344), with a homology of 99%. Two out of three sequences showed variations in ITS2 region. Conclusion: Based on CPT and molecular analysis, T. evansi isolates from infected cattle in South Sulawesi demonstrate genetic diversity of ITS2 sequences.
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31

Nowlan, Godfrey S., Alexander D. McCracken, and Malcolm J. McLeod. "Tectonic and paleogeographic significance of Late Ordovician conodonts in the Canadian Appalachians." Canadian Journal of Earth Sciences 34, no. 11 (November 1, 1997): 1521–37. http://dx.doi.org/10.1139/e17-124.

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Conodonts of Late Ordovician age are well represented in Laurentian parts of eastern Canada, but are rare in the Appalachian orogenic belt. They are known from the Anticosti Basin, the Matapedia Belt, and the Exploits Subzone of the Dunnage Zone. Two new discoveries are described: one from unnamed strata on Markey Brook, west-central New Brunswick, and one from the Goss Point Formation (Avalon Zone, southwestern New Brunswick). Faunas from the Anticosti Basin are of undoubted Midcontinent Faunal Region affinity. Those from the Matapedia Belt are highly mixed both paleoecologically and provincially, containing elements of shallow- and deep-water Midcontinent affinity and elements typical of the Atlantic Faunal Region. Faunas from the Exploits Subzone and Markey Brook are also mixed, suggesting endemic faunas of Atlantic affinity with local influx of Midcontinent faunas from Laurentia. The Goss Point Formation, previously believed to belong to the Mascarene Group (a Silurian cover sequence on the Avalon Zone), also yields mixed faunas. Provincial mixing of all faunas east of the Anticosti Basin suggests that Late Ordovician faunas around Iapetus Ocean may have been more homogeneous than earlier Ordovician faunas. Therefore, the Iapetus Ocean may have been smaller or current patterns may have changed to permit trans-Iapetan migration. A strong global pattern of provincialism for Late Ordovician conodonts is recognized and a new Australasian Province is proposed. Conodonts permit identification of a previously unrecognized Ordovician volcano-sedimentary succession that has economic and tectonic implications for the region.
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Bartholomew, Bruce, Kate E. Armstrong, Rong Li, and Peter W. Fritsch. "Perrottetia taronensis B.M.Barthol. & K.Armstr., sp. nov. (Dipentodontaceae), a new species from northwestern Yunnan Province, China and northern Kachin State, Myanmar and a re-examination of the Asian and Australasian taxa of Perrottetia." PhytoKeys 183 (October 14, 2021): 67–76. http://dx.doi.org/10.3897/phytokeys.183.71505.

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Perrottetia taronensis from the Dulong Jiang valley in northwestern Yunnan Province, China and the Babulongtan mountain range in northern Kachin State, Myanmar is here described as a new species of the Dipentodontaceae. It is the third species of the genus to be recognized for China and the first to be reported for Myanmar. It is similar to P. alpestriss.s. but differs by characters of its leaf margins, inflorescences, and fruit. The three subspecies of P. alpestris recognized by Hou in “Flora Malesiana” are here recognized as three distinct species, i.e., P. alpestris, P. moluccana, and P. philippinensis on the basis of differences in diagnostic characters and distribution. The report in the “Flora of China” of the Taiwan species P. arisanensis from Yunnan is determined to be incorrect due to misidentification of two specimens at KUN.
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33

Vanhoutte, Koenraad, Elie Verleyen, Cathy Kilroy, Koen Sabbe, Renaat Dasseville, and Wim Vyverman. "Catchment characteristics and chemical limnology of small lakes, tarns and mire pools in New Zealand (South Island) and Tasmania." Marine and Freshwater Research 57, no. 1 (2006): 83. http://dx.doi.org/10.1071/mf04276.

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Small alpine water bodies can play a large role in defining patterns of biological and landscape diversity, and may be particularly sensitive to climate change. A large limnological dataset, consisting of 65 and 6 water bodies, respectively, on South Island and Stewart Island (New Zealand) and 76 and 12 water bodies, respectively, in the Tasmanian highlands and coastal areas (Australia), was constructed to assess patterns of variation in alpine and subalpine lakes in the Australasian region. With the exception of the coastal systems, most lakes were very dilute. In general, lake water chemistry resembled world average seawater cationic ratios (WASW). In addition, some New Zealand lakes fell close to the world average freshwater cationic ratios (WAFW), due to relatively high calcium concentrations, and some were dominated by magnesium due to the presence of serpentine bedrock in the catchment area. Multivariate analyses of the joint dataset revealed that the variation in chemical limnological variables was dominated by gradients in conductivity, pH and gilvin. The concurrent relationships between pH, calcium and gilvin, which enabled the differentiation of Tasmanian water bodies into limnological provinces, were absent in New Zealand. In the latter, pH and gilvin contents were not coincident, as clear-water acidic systems occurred in New Zealand. The higher diversity of freshwater bodies in New Zealand will enable independent assessment of the effects of pH and gilvin on the distribution and diversity of biota.
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Suwannapoom, Chatmongkon, Montri Sumontha, Jitthep Tunprasert, Thiti Ruangsuwan, Parinya Pawangkhanant, Dmitriy V. Korost, and Nikolay A. Poyarkov. "A striking new genus and species of cave-dwelling frog (Amphibia: Anura: Microhylidae: Asterophryinae) from Thailand." PeerJ 6 (February 23, 2018): e4422. http://dx.doi.org/10.7717/peerj.4422.

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We report on a discovery ofSiamophryne troglodytesGen. et sp. nov.,a new troglophilous genus and species of microhylid frog from a limestone cave in the tropical forests of western Thailand. To assess its phylogenetic relationships we studied the 12S rRNA–16S rRNA mtDNA fragment with final alignment comprising up to 2,591 bp for 56 microhylid species. Morphological characterization of the new genus is based on examination of external morphology and analysis of osteological characteristics using microCT-scanning. Phylogenetic analyses place the new genus into the mainly Australasian subfamily Asterophryinae as a sister taxon to the genusGastrophrynoides, the only member of the subfamily known from Sundaland. The new genus markedly differs from all other Asterophryinae members by a number of diagnostic morphological characters and demonstrates significant mtDNA sequence divergence. We provide a preliminary description of a tadpole of the new genus. Thus, it represents the only asterophryine taxon with documented free-living larval stage and troglophilous life style. Our work demonstrates thatS. troglodytesGen. et sp. nov.represents an old lineage of the initial radiation of Asterophryinae which took place in the mainland Southeast Asia. Our results strongly support the “out of Indo-Eurasia” biogeographic scenario for this group of frogs. To date, the new frog is only known from a single limestone cave system in Sai Yok District of Kanchanaburi Province of Thailand; its habitat is affected by illegal bat guano mining and other human activities. As such,S. troglodytesGen. et sp. nov.is likely to be at high risk of habitat loss. Considering high ecological specialization and a small known range of the new taxon, we propose a IUCN Red List status of endangered for it.
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Kwiatkowski, Avril. "Enabling quality data reporting: National implementation of standardization pathology reporting." Journal of Clinical Oncology 32, no. 30_suppl (October 20, 2014): 122. http://dx.doi.org/10.1200/jco.2014.32.30_suppl.122.

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122 Background: The Canadian Partnership Against Cancer (the Partnership) launched its Electronic Synoptic Pathology Reporting Initiative (ESPRI) to advance the implementation and promote the wide adoption of standardized synoptic pathology reporting tools in Canada. This presentation will highlight strategies to ensure successful implementation of this program across the Canadian health system as well as lessons learned. Methods: Upon consultation with clinical and system leaders, clear and measureable program targets (such as 90% completeness rates against mandatory elements of the College of American Pathology cancer protocols and disease-specific clinical indicators) were agreed upon. Thorough provincial planning to understand the variances across provincial current states, establish strategies and solidify plans was supported by the Partnership; followed by implementation of those plans. Key approaches to enable adoption and implementation include coordination of Pathologist and Vendor-led education sessions, establishment of clinical expert panels and assignment of Canadian representatives to the College of American Pathologists’ (CAP) Cancer Protocol Review Panels. The Partnership continues to collaborate with national/international standards organizations such as NAACCR, CAP, and Canada Health Infoway to support standards maintenance. Results: As a result of ESPRI, approximately 80% of Canadian pathologists will be reporting synoptically by 2017, across 7 of 10 provinces. It is expected that this will reflect approximately 25,000 reports per year for the five cancer types of focus (breast, colorectal, lung, prostate and endometrial) from about 950 pathologists. The Partnership, with CAP-ACP, has partnered with pathology communities from the US, UK, and Australasia to form the International Collaboration on Cancer Reporting (ICCR) to collaborate on internationally-harmonized core datasets for cancer pathology. Conclusions: Adoption of structured pathology reporting in Canada will enable better patient care, improve data quality, create efficiencies and enable interoperability. Through adoption and implementation efforts, this will impact patient care.
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Pádua, Diego G., Andrés Moreira-Muñoz, Vanezza Morales-Fierro, and Rodrigo O. Araujo. "Chilean Darwin Wasps (Ichneumonidae): Biogeographic Relationships and Distribution Patterns." Insects 15, no. 6 (June 4, 2024): 415. http://dx.doi.org/10.3390/insects15060415.

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Ichneumonidae, or Chilean Darwin wasps, are an important component of South American hymenopteran diversity, but the taxonomic and distributional knowledge on this insect is still deficient. Taking advantage of recently updated taxonomic knowledge, we assessed biogeographic relationships at the genus level and biodiversity spatial patterns along the latitudinal gradient. The results show the presence of 264 species in Chile, arranged in 102 genera and 22 subfamilies. Biogeographic relationships are based on six elements (cosmopolitan (n = 50; 36%), endemic (n = 29; 21%), Neotropical (n = 22; 16%), Holarctic–Oriental (n = 19; 14%), south-temperate (n = 16; 11%) and Australasian) and composed of just three genera: Anacis, Labena, and Meringops. Species and genera show a bimodal distribution along the latitudinal gradient: around 34° and 38° S. From an ecoregional perspective, richness is concentrated in the Valdivian temperate forests, but when assessed at a 0.5 × 0.5 cell scale, several outstanding cells are in the contact zone between the temperate forests and the Chilean Matorral. On the other hand, the Atacama Desert shows little or no presence of Darwin wasps. The results agree with Charles Porter, who identified a northern province composed of Neotropical and cosmopolitan genera with their own representatives in the far north (11 genera), a distributional gap in the core of the Atacama Desert, and around 128 genera in Porter’s Neantarctic realm, covering all of Chile from 25° S to Cape Horn, including the Juan Fernandez islands. These results reinforce knowledge gaps and the need for more sampling and studies of available collections. Due to sampling gaps at this stage, identifying a continued increase or decrease in richness towards higher latitudes is not possible. More taxonomic and distributional information is also needed to assess potential threats to endemic genera and species.
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Luo, Z. W., F. He, H. Y. Fan, X. H. Wang, M. Hua, F. C. Hu, X. H. Li, Z. X. Liu, and N. T. Yu. "First Report of Leaf Spot Disease Caused by Exserohilum rostratum on Pineapple in Hainan Province, China." Plant Disease 96, no. 3 (March 2012): 458. http://dx.doi.org/10.1094/pdis-11-11-0979.

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Pineapple (Ananas comosus (L.) Merr.) is an important perennial monocotyledonous plant that serves as an important fruit crop globally and is also produced in the Hainan Province of China where production in 2009 was 296,600 t. In July 2009, atypical symptoms of a leaf spot disease were observed on mature pineapple leaves in Chengmai County; approximately 15% of plants propagated from suckers became symptomatic after 150 to 300 days, eventually causing a 3 to 10% yield loss. In the initial infection stage, grayish white-to-yellowish white spots emerged on the leaf surfaces that ranged from 1.0 to 2.4 × 0.3 to 0.7 cm; black specks were not always present in the spots. Leaf spots also had distinctive light brown-to-reddish brown banding pattern on the edges. Several spots would often merge to form large lesions, 6.5 to 15.4 × 2.5 to 5.6 cm, covering more than 67% of the leaf surface, which can lead to death of the plant. Infected pineapple leaves collected from an orchard of Chengmai County were surface sterilized (75% ethanol for 30 s, 0.1% HgCl2 for 2 min, and rinsed three times in sterile distilled water). Leaf pieces were placed on potato dextrose agar medium and then incubated at 25°C. The emerging fungal colonies were grayish white to brown. Similar strains were obtained from Qionghai City and Wanning City subsequently. Two isolates, ITF0706-1 and ITF0706-2, were used in confirmation of the identity of the pathogen and in pathogenicity tests. Colonies were fast growing (more than 15 mm per day at 25 to 30°C) with dense aerial mycelia. Conidia were fusiform, pyriform to oval or cylindrical, olive brown to dark brown, 3 to 10 septate (typically 5 to 8), 33.2 to 102.5 × 9.0 to 21.3 μm, with a strongly protruding hilum bulged from the basal cell, which were similar to the Type A conidia described by Lin et al. (3). The strains were subjected to PCR amplification of the internal transcribed spacer (ITS)1-5.8S-ITS2 regions with universal primer pair ITS1/ITS4. The ITS sequence comparisons (GenBank Accession Nos. JN711431 and JN711432) shared between 99.60 and 99.83% identity with the isolate CATAS-ER01 (GenBank Accession No. GQ169762). According to morphological and molecular analysis, the two strains were identified as Exserohilum rostratum (Drechs.) Leonard & Suggs. Pathogenicity experiments were conducted five times and carried out by spraying a conidial suspension (105 CFU/ml) on newly matured leaves of healthy pineapple plants; plants sprayed with sterile water served as the negative control. Plants were incubated in the growth chamber at 20 to 25°C. Symptoms of leaf spot developed on test plants 7 days after inoculation while the control plants remained asymptomatic. Koch's postulates were fulfilled with the reisolation of the two fungal strains. Currently, E. rostratum is one of the most common pathogens on Bromeliads in Florida (2) and has been reported on Zea mays (4), Musa paradisiacal (3), and Calathea picturata (1) in China, but to our knowledge, this is the first report of leaf spot disease caused by E. rostratum on pineapple in Hainan Province of P.R. China. References: (1) L. L. Chern et al. Plant Dis. 95:1033, 2011. (2) R. M. Leahy. Plant Pathol. Circ. No. 393. Florida Department of Agriculture and Consumer Services Division of Plant Industry, 1999. (3) S. H. Lin et al. Australas. Plant Pathol. 40:246, 2011. (4) J. N. Tsai et al. Plant Pathol. Bull. 10:181, 2001.
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Yang, S. Y., S. C. Su, T. Liu, G. Fan, J. Wang, and P. S. Leng. "First Report of Anthracnose Caused by Colletotrichum gloeosporioides on Pistachio (Pistacia vera) in China." Plant Disease 95, no. 10 (October 2011): 1314. http://dx.doi.org/10.1094/pdis-04-11-0269.

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In the 1990s, pistachio (Pistacia vera L. ‘Kerman’ and ‘Peters’) was introduced in China. They are found in many orchards in Beijing and Gansu and Hebei provinces, northern China. In 2009, a new disease was observed on leaves, stems, and fruits in pistachio orchards in Gansu Province. Disease incidence in 8- to 12-year-old orchards was 30%. Yield losses reached 25%. Symptoms began as discrete, sunken, black spots, approximately 10 mm in diameter, followed by circular lesions that eventually coalesced with tissue death recorded and orange fructifications developed on lesions. Pieces of diseased leaves, stems, and fruits were surfaced sterilized and placed on 2% potato dextrose agar (PDA) at 25°C. A fungus was consistently isolated. After 10 days, cultures on PDA showed aerial, white mycelium that turned gray to grayish black with a salmon-to-orange conidial mass at 25°C and a 12-h photoperiod. Brown, 80 to 120 μm long setae were observed in the acervulus. Conidia were hyaline, fusiform to nearly straight, and averaged 12 to 18 × 3 to 5 μm. On the basis of morphological characteristics, the fungus was identified as Colletotrichum gloeosporioides (Penz.) Sacc. (2). On PDA, 0.5 μg/ml of benomyl was applied for the sensitivity test (3). Benomyl completely inhibited the growth of the fungus. Mycelial DNA was extracted, PCR amplified using ITS1 and ITS4 primers for the ribosomal DNA internal transcribed spacers 1 and 2, and sequenced. The DNA sequence was recorded in GenBank as No. HQ631378. The DNA sequence was blasted showing 99% identity with Accession Nos. GQ144454 and GU004376, for C. gloeosporioides. Pathogenicity tests were conducted under greenhouse conditions at 25°C. Three replicates of 2-year-old ‘Kerman’ plants were inoculated with mycelial PDA plugs placed on 0.5-cm2 stem wounds and then wrapped with Parafilm. Controls were inoculated with PDA plugs without the fungus. After 3 weeks, stem cankers were observed on inoculated plants. Control plants remained healthy. Pathogenicity was also tested on injured leaves and fruits. A 10-μl drop of a spore suspension of 104 conidia/ml was applied on ‘Kerman’ and ‘Peters’ leaves and ‘Kerman’ fruits and placed on plates with a wet filter paper at 25°C. Small, black lesions were observed at 2 days after inoculation. At 7 days, necrotic lesions covered the entire surface. C. gloeosporioides was reisolated from necrotic lesions. Controls did not develop symptoms. C. acutatum has been reported on pistachio in Australia (1), but to our knowledge, this is the first report of anthracnose caused by C. gloeosporioides on pistachio. References: (1) G. J. Ash and V. M. Lanoiselet. Australas. Plant Pathol. 30:365, 2001. (2) J. Y. Lu. Plant Pathogenic Mycology. China Agricultural Press, Beijing, 2001. (3) N. A. R. Peres et al. Plant Dis. 86:620, 2002.
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Wu, Entao, Hongchang Wang, Huaxiang Lu, Wenqi Zhu, Yifei Jia, Li Wen, Chi-Yeung Choi, et al. "Unlocking the Potential of Deep Learning for Migratory Waterbirds Monitoring Using Surveillance Video." Remote Sensing 14, no. 3 (January 21, 2022): 514. http://dx.doi.org/10.3390/rs14030514.

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Estimates of migratory waterbirds population provide the essential scientific basis to guide the conservation of coastal wetlands, which are heavily modified and threatened by economic development. New equipment and technology have been increasingly introduced in protected areas to expand the monitoring efforts, among which video surveillance and other unmanned devices are widely used in coastal wetlands. However, the massive amount of video records brings the dual challenge of storage and analysis. Manual analysis methods are time-consuming and error-prone, representing a significant bottleneck to rapid data processing and dissemination and application of results. Recently, video processing with deep learning has emerged as a solution, but its ability to accurately identify and count waterbirds across habitat types (e.g., mudflat, saltmarsh, and open water) is untested in coastal environments. In this study, we developed a two-step automatic waterbird monitoring framework. The first step involves automatic video segmentation, selection, processing, and mosaicking video footages into panorama images covering the entire monitoring area, which are subjected to the second step of counting and density estimation using a depth density estimation network (DDE). We tested the effectiveness and performance of the framework in Tiaozini, Jiangsu Province, China, which is a restored wetland, providing key high-tide roosting ground for migratory waterbirds in the East Asian–Australasian flyway. The results showed that our approach achieved an accuracy of 85.59%, outperforming many other popular deep learning algorithms. Furthermore, the standard error of our model was very small (se = 0.0004), suggesting the high stability of the method. The framework is computing effective—it takes about one minute to process a theme covering the entire site using a high-performance desktop computer. These results demonstrate that our framework can extract ecologically meaningful data and information from video surveillance footages accurately to assist biodiversity monitoring, fulfilling the gap in the efficient use of existing monitoring equipment deployed in protected areas.
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Pho, Nguyen Van, Pham Tich Xuan, and Pham Thanh Dang. "Occurrence of supergene nickel ores in the Ha Tri Massive, Hoa An District, Cao Bang Province." VIETNAM JOURNAL OF EARTH SCIENCES 40, no. 2 (January 19, 2018): 154–65. http://dx.doi.org/10.15625/0866-7187/40/2/11676.

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Nickel (Ni) laterites are regolith materials derived from ultramafic rocks and play an important role in the world's Ni production. Ni-laterite deposits are the supergene enrichment of Ni formed from the intense chemical and mechanical weathering of ultramafic parental rocks. In Vietnam, the weathering profile containing Ni laterite was first discovered in the Ha Tri massive (Cao Bang). This profile develops on the Ha Tri serpentinized peridotite rocks classified to the Cao Bang mafic-ultramafic complex (North Vietnam) and exhibits thick weathered zone (10 - 15m). This work carried out a detailed study of the weathering profile at the center of Ha Tri massive. Samples from different horizons of the profile were collected and analyzed in detail by XRF, XRD and SEM-EDX methods to establish the relationship between the Ni-rich supergene products and the parental peridotites (lherzolite) rocks in Ha Tri massive. The results show that the saprolite horizon is most Ni-rich in the weathering profile in Ha Tri. In this horizon, Ni-silicate minerals of garnierite group such as pimelite, nepouite and other Mg-Ni silicates have been found. The appearance of minerals of garnierite group is due to the exchange of Mg by Ni during weathering of peridotite minerals, especially olivine, which leads to the enrichment of the supergene Ni. The occurrence of Ni silicates suggests the existence of the supergene Ni ore in the weathering profile of the Ha Tri massive.References Bosio N.J., Hurst J.V., Smith R.L., 1975. Nickelliferousnontronite, a 15 Å garnierite, at Niquelandia, Goias Brazil. Clays Clay Miner., 23, 400-403. Brand N.W., Butt C.R.M., Elias M., 1998. Nickel Laterites: Classification and features. AGSO Journal of Australian Geology & Geophysics, 17(4), 81-88. Bricker O.P., Nesbitt H.W. and Gunter W.D., 1973. The stability of talc. American Mineralogist, 58, 64-72. Brindley G.W. and Hang P.T., 1973. The nature of garnierites. Structures, chemical composition and color characteristics. Clay and Clay Minerals, 21, 27-40. Brindley G.W. and Maksimovic Z., 1974. The nature and nomenclature of hydrous nickel-containing silicates. Clay Minerals, 10, 271-277. Brindley G.W. and Wan H.M., 1975. Composition structures and thermal behavior of nickel containing minerals in thelizardite-ne´pouite series. American Mineralogist, 60, 863-871. Brindley G.W., Bish D.L. and Wan H.M., 1979. Compositions, structures and properties of nickel containing minerals in the kerolite-pimelite series. American Mineralogist, 64, 615-625. Cluzel D. and Vigier B., 2008. Syntectonic mobility of supergene nickel ores from New Caledonia (Southwest Pacific). Evidence from faulted regolith and garnierite veins. Resource Geology, 58, 161-170. Colin F., Nahon D., Trescases J.J., Melfi A.J., 1990. Lateritic weathering of pyroxenites at Niquelandia, Goais, Brazil: The supergene behavior ofnickel: Economic Geology, 85, 1010-1023. Das S.K., Sahoo R.K., Muralidhar J., Nayak B.K., 1999. Mineralogy and geochemistry of profilesthrough lateritic nickel deposits at Kansa,Sukinda, Orissa. Joural of Geoogical. SocietyIndia, 53, 649-668. Decarreau A., Colin F., Herbillon A., Manceau A., Nahon D., Paquet H., Trauth-Badaud D.,Trescases J.J., 1987. Domain segregation in NiFe-Mg-Smectites. Clay Minerals, 35, 1-10. Freyssinet P., Butt C.R.M. and Morris R.C., 2005. Oreforming processes related to lateritic weathering. Economic Geology, 100th aniversary volume, 681-722.Garnier J., Quantin C., Martins E.S., Becquer T., 2006. Solid speciation and availability of chromium in ultramafic soils from Niquelandia, Brazil. Journal of Geochemical Exploration, 88, 206-209. Garnier J., Quantin C., Guimarães E., Becquer T., 2008. Can chromite weathering be a source of Cr in soils? Mineralogy Magazine, 72, 49-53. Gleeson S.A., Butt C.R. and Elias M., 2003. Nickel laterites: A review. SEG Newsletter, 54, 11-18. Gleeson S.A., Butt C.R., Wlias M., 2003. Nickellaterites: a review. SEG Newsletter, Society of Economic Geology, 54. Available from www.segweb.org. Golightly J.P., 1981. Nickeliferous laterite deposits. Economic Geology, 75th Anniversary volume, 710-735. Golightly J.P., 2010. Progress in understanding the evolution of nickel laterite. Society of Economic Geology, In Special Publication, 15, 451-485. Manceau A. and Calas G., 1985. Heterogeneous distribution of nickel in hydrous silicates from New Caledonia ore deposits. American Mineralogist, 70, 549-558. Nguyen Van Pho, 2013. Tropic weathering in Vietnam (in Vietnamese). Pubisher Science and Technology, 365p.Ngo Xuan Thanh, Tran Thanh Hai, Nguyen Hoang, Vu Quang Lan, S. Kwon, Tetsumaru Itaya, M. Santosh, 2014. Backarc mafic-ultramafic magmatism in Northeastern Vietnam and its regional tectonic significance. Journal of Asian Earth Sciences, 90, 45-60.Pelletier B., 1983. Localisation du nickel dans les minerais ‘‘garnieritiques’’ de Nouvelle-Caledonie. Sciences Ge´ologique: Me´moires, 73, 173-183.Pelletier B., 1996. Serpentines in nickel silicate ores from New Caledonia. In Grimsey E.J., and Neuss I. (eds): Nickel ’96, Australasian Institute of Miningand Metallurgy, Melbourne, Publication Series 6(9), 197-205. Proenza J.A., Lewis J.F., Galı´ S., Tauler E., Labrador M., Melgarejo J.C., Longo F. and Bloise G., 2008. Garnierite mineralization from Falcondo Ni-laterite deposit (Dominican Republic). Macla, 9, 197-198. Soler J.M., Cama J., Galı´ S., Mele´ndez W., Ramı´rez, A., andEstanga, J., 2008. Composition and dissolution kinetics ofgarnierite from the Loma de Hierro Ni-laterite deposit,Venezuela. Chemical Geology, 249, 191-202. Springer G., 1974. Compositional and structural variations ingarnierites. The Canadian Mineralogist, 12, 381-388. Springer G., 1976. Falcondoite, nickel analogue of sepiolite. The Canadian Mineralogist, 14, 407-409.Svetlitskaya T.V., Tolstykh N.D., Izokh A.E., Phuong Ngo Thi, 2015. PGE geochemical constraints on the origin of the Ni-Cu-PGE sulfide mineralization in the Suoi Cun intrusion, Cao Bang province, Northeastern Vietnam. Miner Petrol, 109, 161-180.Tran Trong Hoa, Izokh A.E., Polyakov G.V., Borisenko A.S., Tran Tuan Anh, Balykin P.A., Ngo Thi Phuong, Rudnev S.N., Vu Van Van, Bui An Nien, 2008. Permo-Triassic magmatism and metallogeny of Northern Vietnam in relation to the Emeishan plume. Russ. Geol. Geophys., 49, 480-491.Trescases J.J., 1975. L'évolution supergene des roches ultrabasiques en zone tropicale: Formation de gisements nikelifères de Nouvelle Caledonie. Editions ORSTOM, Paris, 259p.Tri T.V., Khuc V. (eds), 2011. Geology and Earth Resources of Vietnam. Publishing House for Science and Technology, 645p (in English). Villanova-de-Benavent C., Proenza J.A., GalíS., Tauler E., Lewis J.F. and Longo F., 2011. Talc- and serpentine-like ‘‘garnierites’’ in the Falcondo Ni-laterite deposit, Dominican Republic. ‘Let’s talk ore deposits’, 11th Biennial Meeting SGA 2011, Antofagasta, Chile, 3p.Wells M.A., 2003. Goronickel laterite deposit. New Caledonia. CRC LEME, p.3.
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Wu, Lan, Yuyu Wang, Xunqiang Mo, Qian Wei, Chaohong Ma, Hao Wang, Terry Townshend, Yifei Jia, Wenjia Hu, and Guangchun Lei. "Shifted to the South, Shifted to the North, but No Expansion: Potential Suitable Habitat Distribution Shift and Conservation Gap of the Critically Endangered Baer’s Pochard (Aythya baeri)." Remote Sensing 14, no. 9 (April 30, 2022): 2171. http://dx.doi.org/10.3390/rs14092171.

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There are many challenges in biodiversity conservation, especially for migratory waterbirds because their survival depends on the availability of a suite of interconnected sites at different stages of their annual cycle. Due to habitat loss and degradation, the population of Baer’s Pochard (Aythya baeri), a migratory diving-duck, has declined to an extent that it has been listed as a critically endangered species by the IUCN Red List. To better understand the habitat requirements of this threatened waterbird for its effective conservation, we conducted seven years of field surveys within its historical distribution range in the East Asian–Australasian Flyway including China and neighbouring countries, covering 563 observation sites at 185 locations. Twelve new locations were identified as habitats for this species. By combining our surveys with literature and citizen science birding records, 171 Baer’s Pochard’s presence sites have classified as migratory stopovers, wintering grounds and breeding and potential breeding habitats. We then used Maxent model to estimate the potential distribution range and updated and refined the current IUCN distribution map. Finally, we identified the key conservation gaps by overlaying the distribution with a recent remotely acquired global landcover map. Our results show that: (1) The southernmost breeding site is about 1400 km south of its current IUCN breeding range; (2) the northern most wintering site is 800 km north of the IUCN wintering range; (3) Six newly discovered sites in Hebei, Henan, Shandong, Jiangxi and Hubei provinces, China are confirmed to be used all year round; (4) Most sites (81.8%) are not located in protected areas (PAs), and the majority of the suitable habitats (90%) are not protected by the current PA network. Our findings reveal that great changes have taken place in the distribution of Baer’s Pochard and that there are many distribution overlaps throughout its annual migration circle (e.g., many historical stopover sites become breeding habitats). Moreover, the key habitats have retreated into eastern Asia, and most of the habitats overlap with urban developed areas and are outside of current PA network. Our study suggests that the existing PA network may be less effective for the conservation of this critically endangered species under predicted global climate change, and other effective area-based conservation measures should be part of the conservation strategy. More importantly, as the distribution of Baer’s Pochard covers at least 15 countries, closely coordinated cross-border cooperation would be critical for its future survival.
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42

Yan, J. Y., Y. L. Peng, Y. Xie, X. H. Li, S. W. Yao, M. L. Tang, and Z. Y. Wang. "First Report of Grapevine Trunk Disease Caused by Botryosphaeria obtusa in China." Plant Disease 95, no. 5 (May 2011): 616. http://dx.doi.org/10.1094/pdis-11-10-0821.

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In September 2010, grapevine (Vitis vinifera) trunk diseases were observed in several vineyards of Yantai District in Shandong Provinces and Changli County of Hebei Provinces of China. Characteristic symptoms of Botryosphaeria canker were apparent, including dark brown discoloration on the trunk (visible in cross-section), cob base shriveling, drying of fruit clusters, and berry falling (2). To identify the causal pathogen, culturing of fungi was attempted from 387 small pieces of tissue from the canker margins of 43 diseased plants. Samples were surface disinfected by placing them in 75% ethanol for 1 min and rinsing with sterilized water three times before culturing on potato dextrose agar (PDA) at 28°C for 7 to 10 days. Fungi isolated were single spored to obtain pure cultures. On the basis of colony characteristics on PDA, 18 isolates from the 387 tissue pieces were eventually identified as Botryosphaeria obtusa (1), Most of the other fungi isolated were B. dothidea. B. obtusa colonies were grayish white, becoming dark brown with age, and pycnidia were formed after incubation for approximately 7 days. Conidia measured 8 to 11 × 17 to 26 μm (n= 50). Two isolates were used for rDNA internal transcribed spacer (ITS) sequence analysis with primers ITS1 and ITS4 (3). PCR products were separated by electrophoresis and bands were purified for legation with PMD-18T (Takara Company, Dalian, China) vector for sequencing. BLAST searches of two ITS sequences had 99 to 100% identity to B. obtusa. EF1-α and β-tubulin sequence analysis gave similar results. Koch's postulates were completed in the greenhouse on grape shoots inoculated with two isolates of B. obtusa originally isolated from diseased plants in the field. Inoculations were made on green shoots of V. vinifera cv. Dunkelfelder T. Six shoots were inoculated per isolate by wounding with a 4-mm cork borer (2 mm deep) and placing a colonized agar plug from a 5-day-old culture on the wound and wrapping it with Parafilm. Controls were mock inoculated with an agar plug from sterile PDA. Inoculated shoots were incubated in the dark under moist conditions in the laboratory for 8 to 10 days at 25°C. Inoculated shoots had necrotic cankers after 8 to 10 days and B. obtusa was recovered from each canker margin. The results indicated that some grapevines in China with symptoms of Botryosphaeria canker were infected by B. obtusa. To our knowledge, this is the first report of this pathogen causing trunk disease on grapevine in China. References: (1) A. Taylor et al. Australas. Plant Pathol. 34:187, 2005. (2) J. R. Úrbez-Torres et al. Plant Dis. 92:519, 2008. (3) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.
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Li, H. L., H. X. Yuan, J. W. Sun, B. Fu, G. L. Nian, X. S. Hou, X. P. Xing, and B. J. Sun. "First Record of the Cereal Cyst Nematode Heterodera filipjevi in China." Plant Disease 94, no. 12 (December 2010): 1505. http://dx.doi.org/10.1094/pdis-04-10-0301.

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Cereal cyst nematode (CCN) is now recognized as a widespread and often damaging parasite of wheat in China. Only Heterodera avenae has been reported in China (4). However, molecular analysis of four samples from Beijing and one from Shanxi Province indicated genetic differences from H. avenae and other named species (3). Here we report the detection of H. filipjevi at a site in Henan Province that was not included in any previous study or report. The infested crop was rainfed winter wheat (Triticum aestivum) cv. Wenmai 19 in a field near Banpopu Village in Xuchang County (34.0447°N, 113.7415°E) with a long-established maize-wheat semiannual crop rotation. During the winter growing season, the crop was patchy with uneven growth and cyst nematode females were observed on the roots. In June 2009, soil was collected and mature cysts were extracted for morphological and molecular identification. Cysts were also kept at 4°C for 2 months and then incubated in shallow water at 15°C for a month to obtain second-stage juveniles (J2). Measurements (range; mean ± sd) of 10 cysts were body length including neck (569 to 786 μm; 699 ± 56), body width (403 to 600 μm; 523 ± 55), length:width ratio (1.3 to 1.5; 1.3 ± 0.1), neck length (61 to 125 μm; 106 ± 19) and width (49 to 83 μm; 69 ± 13), fenestra length (52 to 59 μm; 57 ± 2.9) and width (24.5 to 34.4; 27.9 ± 3.5), underbridge (64 to 101 μm; 85 ± 10), and vulval slit (7.4 to 10.0 μm; 9.6 ± 1.0). Lemon-shaped cysts were brown with a surface zigzag pattern. The vulval cone was bifenestrate with horseshoe-shaped semifenestra, with heavy underbridge and many bullae. The J2 (n = 22) measurements were body length (496 to 590 μm; 552 ± 24), body width (20.0 to 23.8; 21.5 ± 0.9), stylet (22.8 to 25.3; 24.0 ± 1.0) with anchor-shaped basal knobs, tail (47 to 64; 61.6 ± 4.4), and hyaline tail terminus (32 to 43; 40.2 ± 3.0). The J2 had up to four lateral lines, but the inner two were often the only lines clearly visible, and the shape of the stylet knobs, tail, and tail terminus were consistent with H. filipjevi. All morphological data and characters were consistent with H. filipjevi (1). Specimens have been lodged with the Australian National Insect Collection. DNA from single cysts was extracted to amplify the internal transcribed spacer region of rDNA by PCR with forward primer TW81 (5′-GTTTCCGTAGGTGAACCTGC-3′) and reverse primer AB28 (5′-ATATGCTTAAGTTCAGCGGGT-3′) (2). The PCR product was sequenced (Genbank Accession No. HM027892) and digested by restriction enzymes (AluI, CfoI, HaeI, HinfI, PstI, RsaI, TaqI, and Tru9I) to obtain restriction fragment length polymorphism profiles (2). Profiles for the Xuchang population consistently matched those published for H. filipjevi and were distinct from those of H. avenae and other species (3). Phylogenic analysis of the sequence further indicated conspecificity with H. filipjevi. These morphological and molecular data confirmed that the specimens from Xuchang were H. filipjevi, which represents the first detection of H. filipjevi in China, and extends the known distribution of the species from Europe, North America, South Asia, and West Asia to East Asia. This finding adds complexity to the management of CCN in China, especially for control by host resistance, which now must consider both species and pathotype diversity. References: (1) Z. A. Handoo. J. Nematol. 34:250, 2002. (2) S. A. Subbotin et al. Nematology 2:153, 2000. (3) S. A. Subbotin et al. Nematology 5:515, 2003. (4) H. X. Yuan et al. Australas. Plant Pathol. 39:107, 2010.
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44

Wang, Y., K. D. Xu, Y. Zhang, K. Liu, F. L. Zhang, J. Zhang, G. X. Tan, and C. W. Li. "First Report of Powdery Mildew Caused by Erysiphe heraclei on Carrot in Central China." Plant Disease 98, no. 2 (February 2014): 277. http://dx.doi.org/10.1094/pdis-04-13-0439-pdn.

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Carrot (Daucus carota) is an important root vegetable crop in China, which accounted for 46% of global production in 2011. Carrot was grown in Henan Province on >20,000 ha/year, which ranks first in China for area of carrots harvested. In October 2012, a powdery mildew outbreak was observed in 16 investigated carrot production fields in Zhoukou, Henan Province, in central China. White colonies typical of powdery mildew were seen on leaves of affected plants. The colonies enlarged and finally coalesced. Small, scattered fruiting bodies found on the adaxial and abaxial leaf surfaces were determined microscopically to be chasmothecia. Examining the pathogen morphologically revealed that appressoria were lobed, conidiophores were straight and bore single conidia, and cylindrical foot cells were followed by one to three shorter cells in the conidiophores. Conidiophores were subhyaline and 54.1 to 66.1 × 6.1 to 8.1 μm. Conidia were barrel-cylindrical and 28.8 to 38.6 × 11.4 to 14.8 μm. Chasmothecia were subspherical, dark brown to black, formed hyphoid appendages, and 110 to 122 μm in diameter. Appendages typically had one to five branches, which were nearly dichotomous or irregular, flexuous or almost straight, and 30 to 165 μm long. Each chasmothecium contained multiple asci that were saccate, multiguttulate, short-stipitate or not, 62.5 to 63.8 × 43.2 to 45.9 μm, and each contained two to six ascospores. Ascospores were subhyaline, ovoid to ellipsoid, and 16.5 to 17.7 × 11.2 to 12.7 μm. Based on characteristics of the anamorphic and teleomorphic stages, the fungus was identified as Erysiphe heraclei (2,4). To verify the identity, the internal transcribed spacer (ITS) region of ribosomal DNA was amplified with universal primers ITS1 and ITS4, and sequenced. The ITS sequence was assigned GenBank Accession No. KC480605, and showed 100% similarity to ITS sequences of E. heraclei on carrot in GenBank (EU371725 and GU252368). Koch's postulates were completed by using detached infected leaves from 10-week-old carrot plants growing in a field to inoculate 10 healthy, 5-week-old plants of the carrot cultivar Dinghong, growing in a growth chamber under 22/16°C (day/night) cycle at 50% relative humidity with 120 μmol/m2/s light and a 14-h photoperiod. Ten non-inoculated plants served as replicates of a control treatment. Symptoms consistent with those in the field were observed on inoculated plants 20 days post-inoculation. No symptoms were observed on the control plants. Microscopic observation of the pathogen growing on the inoculated plants revealed that it was the same as the original fungus. Powdery mildew on carrot has been observed in many countries including Australia (1), Mexico (3), and the United States (2). To our knowledge, this is the first report of E. heraclei infection on carrot in central China, a major region of carrot production, although the disease has previously been observed in northwestern China (4). Further research should help to reduce losses in carrot crops caused by E. heraclei in central China. References: (1) J. H. Cunnington et al. Australas. Plant Dis. Notes 3:38, 2008. (2) D. A. Glawe et al. Plant Health Progress doi: 10.1094/PHP-2005-0114-01-HN, 2005. (3) G. Rodríguez-Alvarado et al. Plant Dis. 94:483, 2010. (4) R. Zheng and G. Chen. Pp. 97-99 in: Flora Fungorum Sinicorum Vol. 1. Erysiphales. R. Zheng et al., eds. Science Press, Beijing, 1987.
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45

Zhang, M. X., L. F. Zhai, W. X. Xu, N. Hong, and G. P. Wang. "First Report of Valsa leucostoma Causing Valsa Canker of Pyrus communis (cv. Duchess de' Angouleme) in China." Plant Disease 98, no. 3 (March 2014): 422. http://dx.doi.org/10.1094/pdis-07-13-0704-pdn.

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Pear is a popular fruit in the world market, and has been widely cultivated in China. Since 2008, a severe canker disease has consistently been observed on 20-year-old pear trees (Pyrus communis cv. Duchess de' Angouleme) grown in a nursery in Xingcheng, Liaoning Province, China. Observed symptoms include brown elongated ulcerative lesions (more than 20 cm in length in general), with red brown conidia produced on wet lesions. Reductions in tree vigor and yield were observed for infected trees. Tree mortality was observed for severe infections. To diagnose the pathogen, 15 canker samples were collected from five pear trees in April, 2012. Bark pieces (3 to 5 mm) taken from the border of healthy and diseased tissue were surface-disinfected with 0.1% mercury bichloride and 75% ethanol for 45 s, and placed on potato dextrose agar (PDA) medium at 25°C in darkness. Fungal colonies with a common colony morphology were consistently recovered from three samples. These fungal colonies were initially white, becoming olive green in 3 days. Conidia produced on colonies were hyaline, allantoid, and single-celled with average length × width of 6.04 (5.43 to 6.59) × 0.65 (0.51 to 0.73) μm, which were consistent with descriptions of Valsa leucostoma (1). Genomic DNA was extracted from a representative isolate F-LN-32b, and subjected to PCR amplification of the internal transcribed spacer region (ITS), β-tubulin gene, and EF1 gene using the primer pairs ITS1/ITS4, Bt2a/Bt2b and EF1-728F/EF1-986R (3), respectively. Sequence alignment of the amplified fragments with the deposited data in NCBI showed that sequences of EF1, ITS, and β-tubulin (GenBank Accession No. KF293296 to KF293298, respectively) of isolate F-LN-32b had the highest similarity of 99% to those of V. leucostoma strain 32-2w (JQ900340, JN584644, and JQ900374), and suggested that isolate F-LN-32b is a V. leucostoma strain. Pathogenicity tests was carried out by placing a 5-mm-diameter, 2-day-old mycelium agar plug of isolate F-LN-32b onto a punched bark hole of a detached 1-year-old pear shoot after it was surface disinfested with ethanol. Inoculated shoots were incubated at 25°C in plastic containers covered with plastic film. Pathogenicity assays were conducted on 18 pear varieties (cvs. Qiuyue, Jinshui 2, Hohsui, Huali 1, Cuiguan, Shinseiki, Xuehua, Dangshansu, Zaosu, Hongxiangsu, Yuluxiang, Nanguoli, Xizilv, Bartlett, Huanghua, Huashan, Duchess de' Angouleme, and Packham's) collected from a nursery in Wuhan, Hubei Province, China. Six shoots were inoculated for each variety and the assay was conducted three times. All inoculated shoots developed the typical canker symptoms after 6 days post inoculation (dpi) and sporulated at 25 dpi while the control shoots inoculated with non-colonized PDA plugs remained asymptomatic. Isolates recovered from inoculated samples were of the same morphology and ITS sequence as F-LN-32b. Based on these results, V. leucostoma was determined as the pathogen responsible for the Valsa canker disease on pear. Valsa mali var. pyri was identified as the only pathogen causing Valsa canker disease on pear in China (2). To our knowledge, this is the first report of V. leucostoma causing a canker disease on pear in China. References: (1) G. C. Adams et al. Australas. Plant Pathol. 35:521, 2006. (2) X. L. Wang et al. Mycologia 103:317, 2011. (3) T. J. White et al. Pages 315-322 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.
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46

Pérez, C. A., R. Reyna, L. Montanari, D. Torres-Dini, N. Nikichuk, and S. Simeto. "First Report of Rust Caused by Puccinia psidii on Eucalyptus dunnii in Uruguay." Plant Disease 98, no. 10 (October 2014): 1444. http://dx.doi.org/10.1094/pdis-07-14-0700-pdn.

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Eucalypt rust caused by Puccinia psidii Winter represents a major disease affecting eucalypt production in South America, and is threatening myrtaceous hosts worldwide. In Uruguay, it was first detected infecting Eucalyptus globulus in 2001 (4) and later on E. grandis, Myrrhinium atropurpureum, and Myrcianthes pungens, two myrtaceae hosts native to Uruguay (3). Over the summer 2013, bright orange-yellowish pustules were detected on stock plants of E. dunnii in a nursery located in western Uruguay, province of Paysandú. A severe and explosive epidemic was readily observed. Affected leaves were taken to the laboratory and examined under the microscope. Uredinia and urediniospores were similar with those described previously in Uruguay on other hosts (3,4). Uredioniospores were 18 to 25 × 15 to 21 μm, yellow, unicellular, spherical to elliptical, base truncate, finely and uniformly echinulate with spines up to 1 μm long, with an evident bald patch without spines. To confirm identity of this fungus, genomic DNA was extracted from single-pustule urediniospores, and ITS region was amplified using primers PR1 and PR2 (1) with PCR conditions previously described (3). Forward and reverse sequences from three single pustules were obtained, assembled, and compared with those available in GenBank using BLAST searches. Obtained sequences showed to be identical to those analyzed by Pérez et al. (2011) collected from E. grandis and E. globulus, with no variation found in the analyzed region. A 100% identity was found with isolates UY1374 and UY1375 obtained from E. globulus, and with isolate UY1731 obtained from E. grandis (FJ710805, FJ710806, and FJ710807, respectively). Sequences were deposited in GenBank with accession numbers KM083129, KM083130, and KM083131. Even though this pathogen is known to occur in a wide variety of myrtaceous species, to our knowledge, this is the first natural infection record of P. psidii on E. dunnii in Uruguay and worldwide. This report confirms previous studies that indicated the susceptibility of E. dunnii when inoculated under controlled conditions (2,5). E. dunnii is being widely planted in Uruguay, eastern Argentina, and southern Brazil, with increasing significance to the pulp industry. Although the consequences of this finding have yet to be realized, scouting plantations is essential to estimate the real impact of this pathogen on this tree species. Our study contributes to expand the host range known for this globally important pathogen. References: (1) S. R. H. Langrell et al. Plant Pathol. 57:687, 2008. (2) L. Morin et al. PLoS ONE 7:e35434, 2012. (3) C. A. Pérez et al. Mycol. Progress 10:273, 2011. (4) N. Telechea et al. Plant Pathol. 52:427, 2003. (5) E. A. V. Zauza et al. Australas. Plant Pathol. 39:406, 2010.
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47

Icoz, S. M., I. Polat, G. Sulu, M. Yilmaz, A. Unlu, S. Soylu, I. A. Bozkurt, and Ö. Baysal. "First Report of Bacterial Blight of Pomegranate Caused by Xanthomonas axonopodis pv. punicae in Turkey." Plant Disease 98, no. 10 (October 2014): 1427. http://dx.doi.org/10.1094/pdis-06-14-0656-pdn.

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Pomegranate (Punica granatum L.) is an increasingly important fruit crop that is widely cultivated in Turkey. Typical bacterial blight symptoms were observed since spring of 2011 in pomegranate orchards located in Antalya Province. Symptoms were characterized by dark brown, angular to irregularly shaped spots on leaves and fruit; cankers on stems, branches, and trunks; and split trunks. The pathogen was isolated from leaf spots on naturally infected plants showing typical symptoms onto yeast dextrose chalk agar. Bright yellow bacterial colonies were consistently isolated. Bacterial strains were characterized as gram negative, oxidase negative, catalase positive, tobacco hypersensitivity positive, and able to produce acid from L-arabinose, D-galactose, D-glucose, and D-mannitol but not from D-xylose. Pathogenicity of the representative bacterial strain Serik-4 was performed on 2-year-old pomegranate plants cv. Hicaz. Leaves were sprayed until runoff with bacterial cell suspensions containing 107 CFU/ml. Inoculated plants were covered with transparent plastic bags to maintain moisture for 48 h. Negative control plants were inoculated with sterile distilled water. Plants were then incubated in a greenhouse at 30°C for 14 days. Symptoms on leaves included dark brown, angular to irregularly shaped water soaked lesions along the veins of the inoculated plants 10 days after inoculation. No lesions developed on the control plants. The symptoms on inoculated plants were similar to those on naturally infected plants. Yellow bacterial colonies were re-isolated from the inoculated plants and identified as the same as the original strain by conventional tests and FAME analysis, thus fulfilling Koch's postulates. Fatty acid methyl ester profiling of the representative strain Serik-4 using GC-MIDI (Microbial Identification Inc, Newark, DE) identified the genus of the bacterium as Xanthomonas. The identity of Serik-4 was further confirmed by amplifying the 16S rRNA gene with the universal primers 27F and 1492R (3) and sequence analysis (GenBank Accession No. KM007073). The 16S rRNA gene sequences of Serik-4 was 99% identical to the corresponding gene sequences of the Xanthomonas axonopodis pv. punicae strain present in the NCBI database (JQ067629.1). High incidence of bacterial blight caused by X. axonopodis pv. punicae on pomegranate has been previously reported in India (2), Pakistan (1), and South Africa (4). To our knowledge, this is the first report of bacterial blight on pomegranate caused by X. axonopodis pv. punicae in Turkey. References: (1) M. A. Akhtar and M. H. R. Bhatti. Pakistan J. Agric. Res. 13:95, 1992. (2) R. Chand and R. Kishun. Indian Phytopathol. 44:370, 1991. (3) D. J. Lane. Page 115 in: Nucleic Acid Techniques in Bacterial Systematics, 1991. (4) Y. Petersen et al. Australas. Plant Pathol. 39:544, 2010.
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48

Dong, W. H., and Y. B. Bian. "Stipe Canker Caused by Trichothecium roseum on the Edible Shaggy Mane Coprinus comatus in China." Plant Disease 97, no. 11 (November 2013): 1507. http://dx.doi.org/10.1094/pdis-03-13-0344-pdn.

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Coprinus comatus, the shaggy mane, is one of the most popular and widely cultivated edible mushrooms in China. Its young fruiting body has good nutritional and medicinal value as well as a special flavor. In July 2010, an unusual stipe rot symptom was observed in cultivation tunnels in Pingyin county of Shandong Province. The lower part of the stipe was infected and water soaked scab occurred. The scab then expanded, a few mycelia and pink spores emerged on the scab surface, and finally, the stipe decayed and the fruiting body became wilted. The pathogen was isolated from infected tissues of C. comatus and the colonies on CYM were whitish at first, then pink sorus emerged, later forming concentric rings of sporulation. Mycelia were floccose, colorless, slender, and septate. Conidiophores bore upright, nonbranched, and colorless sporogenous cells, and slightly rounded spores were borne on the top and aggregated in pink cephaloid. Conidia were obovoid or obpyriform. Almost all spores were two-celled and one septate, while one was nearly round and the other bore an apex (1). The size of spores ranged from 10 to 21 μm long and 5 to 7 μm wide, which was consistent with the characteristics of Trichothecium roseum (1). The species identification was confirmed by sequencing the ribosomal ITS sequences. The ribosomal ITS1-5.8S-ITS2 region was amplified from the isolated strain using primers ITS1 and ITS4. A BLAST search in GenBank revealed the highest similarity (99%) to T. roseum (JQ434580). Pathogenicity was tested on different parts of 20 fruiting bodies of C. comatus with or without the wound treatment. One inoculum was prepared by flooding the agar surface with sterilized double distilled water for spore suspension (6.5 × 103 conidia/ml), and the other was by 0.2 × 0.2 cm mycelial plugs without spore production on CYM at 25°C for 5 days. After 1 to 2 days, only inoculated stipes showed water soaked and slight decay on the injured surface of all 20 fruiting bodies, while control fruiting bodies remained healthy. The symptoms were similar to those observed in the cultivation tunnels. No symptom was observed on the pileus, either with or without wound treatment. Pathogens reisolated from the inoculated stipes were confirmed to be T. roseum based on morphological characteristics. Because T. roseum is generally regarded as a postharvest disease of fruits and vegetables such as apple, pear, and muskmelon (2), apples and pears were inoculated with this fungus as well using the same methods. The parts inoculated were sunken, wettish, and decayed with brown stain at 25°C and 90% relative humidity after 5 days. Thus, it was confirmed that the T. roseum from the C. comatus stipe canker could infect the fruits of apple and pear. To our knowledge, this is the first report that T. roseum can cause disease on agaric. References: (1) G. Dal Bello. Australas. Plant Dis. Notes 3:103, 2008. (2) J.-H. Kwon et al. Plant Pathol. J. 26:296, 2010.
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49

Tan, Z. W., C. S. Wang, and G. L. Wang. "First Report of Bark Cracking of Koelreuteria bipinnata var integrifoliola Caused by Lasiodiplodia theobromae in China." Plant Disease 96, no. 10 (October 2012): 1579. http://dx.doi.org/10.1094/pdis-04-12-0337-pdn.

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Koelreuteria bipinnata var integrifoliola is becoming a popular urban green tree in Ningbo City, Zhejiang Province, China, because of its adaptation ability to local conditions, fast growth, and beautiful appearance. A survey conducted from 2007 to 2010 revealed serious bark cracking on greenbelt trees approximately 15 to 16 years old that had been transplanted 5 to 6 years ago. Bark cracks increased in size over time, extending into the phloem and leading to extensive areas of bark loss with discoloration of the underlying xylem. Symptomatic trees had fewer new shoots in spring; many wilted and died in summer. Root rot was not observed in the withered trees but large light brown lesions were observed on cross sections of the main stem, each with a dark brown outer margin. In a September 2009 survey, 95% of symptomatic trees had stem lesions more than 50 cm long. Pieces of xylem (2 × 2 × 1 mm thick) were obtained from the margin of lesions surface sterilized using 0.1% mercuric chloride for 30 s, washed in sterile distilled water, and placed on 2% potato dextrose agar (PDA) at 28°C for 2 days. The fungus was then isolated and 12 colonies were obtrained. Three isolates KL-1-2, KL-3-2, and KL-4-3 were incubated on 2% PDA at 28°C for 30 days to produce spores. On PDA, the colonies were circular or near circular with irregular gray edges turning black green or black. The fungus also produced abundant aerial hyphae that were villous, septate, and irregular branched. Conidia were elliptical (or rounded) and hyaline when immature, becoming dark brown and septate longitudinally when mature and ranged from 23.2 to 27.0 × 10.8 to 16.2 μm (average 25.3 × 13.6 μm), similar to Lasiodiplodia theobromae (Patouillard) Griffon =Botryodiplodia theobromae Pa.t, Botryosphaeria rhodina (Berkeley & Curtis) von Arx (2). DNA extraction directly from the mycelium of KL-1-2, KL-3-2, and KL-4-3 was performed after 10 days' growth on PDA (1). The identities of the three isolates were confirmed by ITS1-5.8S-ITS2 rDNA sequence (GenBank Accession Nos. JN681172, JQ894322, and JQ894323, respectively) analysis that showed 99%, 100%, and 100% sequence similarity to L. theobromae xsd08006 (Accession No. FJ478102), L. theobromae PD20 (Accession No. GU251120), and L. theobromae xsd08008 (Accession No. EU918707), respectively. Pathogenicity tests were performed on 20 five-year-old K. bipinnata var integrifoliola plants by placing mycelia plugs of isolate KL-1-2 (10 × 10 mm) on the main trunk after wounding with a metal needle. Control plants received PDA plugs without mycelium. After inoculation, humidity was maintained using wet absorbent cotton and PE wrap film. Stem bark and phloem cracking was observed after 60 days on 85% of inoculated plants; 30% of those trees also had xylem discoloration. Symptoms were similar to those with natural infection. Control plants remained symptomless. The same fungus was reisolated from the brown xylem of inoculated plants. To our knowledge, this is the first report of bark cracking of K. bipinnata var integrifoliola caused by L. theobromae in China. References: (1) M.-J. Côté et al. Plant Dis. 88:1219, 2004. (2) G. Fu et al. Australas. Plant Dis. Notes 2:75, 2007.
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50

Park, C. Y., M. A. Lee, M. Nam, E. H. Park, Y. S. Bae, S. H. Lee, J. S. Kim, and D. Y. Won. "First Report of Clover yellow vein virus on White Clover (Trifolium repens) in South Korea." Plant Disease 98, no. 10 (October 2014): 1450. http://dx.doi.org/10.1094/pdis-05-14-0540-pdn.

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White clover (Trifolium repens L.) is a herbaceous, perennial plant that has become one of the most widely distributed legumes in the world. It is extensively used in grass-legume pastures, but also has the potential to invade agricultural lands and natural ecosystems. White clover is a well-known natural host for Alfalfa mosaic virus (AMV), Clover yellow vein virus (ClYVV), Soybean dwarf virus (SbDV), Beet western virus (BWYV), Tomato spotted wilt virus (TSWV), Zucchini yellow mosaic virus (ZYMV), etc (1). In July 2013, during a survey to determine the presence of different viruses infecting weed plants in South Korea, three white clover leaf samples showing yellow mosaic symptoms were collected from Taean County, South Chungcheong Do Province, South Korea. In order to identify the infecting virus, total RNA from three leaf samples was extracted using the Tri-reagent (MRC Reagent, Inc., OH) as described by the manufacturer, and was applied to the large-scale oligonucleotide (LSON) chip (3), wherein probes specific to a ClYVV isolate produced a positive reaction. All three samples tested were positive for ClYVV. To confirm this result, ClYVV-specific primers were designed using the sequences of four ClYVV isolates from NCBI (GenBank Accession Nos. AF185959, AF203536, DQ333346, and NC003536). Total RNA was extracted from symptomatic white clover samples using Easy-Spin Total RNA Extraction Kit (iNtRon, Daejeon, Korea) and used as template for RT-PCR. The positive control RNA was used from ClYVV GM isolate (KF975894) and negative control RNA used symptomless white clover plants. The ClYVV coat protein (CP) gene was amplified by RT-PCR using the specific primer pairs ClYVV-CP-F / ClYVV-CP-R (5′-CAAGAGCAGCACGATGAG-3′ and 5′-CTCGCTCTATAAAGATCAGAT-3′). DNA fragments of the expected size (1,042 bp) were obtained from the white clover Korea isolate (AB930132), and the PCR product was cloned into a T&A cloning vector (RBC Bioscience, Taipei, Taiwan) and sequenced directly in both directions. BLAST analyses of the nucleotide sequence CP gene fragments revealed the highest identity with 98% with other ClYVV isolates (AF203536). To determine the experimental host range of the ClYVV Korea isolate, we inoculated five species (Chenopodium amaranticolor, C. quinoa, Nicotiana clevelandii, N. benthamiana, and Trifolium repens) in three families using this isolate. All test plants were mechanically inoculated with 0.1 M phosphate buffered saline (Takara, Tokyo, Japan). Each test plant was inoculated nine times and grown in a greenhouse maintained at 27 to 33°C. Necrotic local lesions were produced on inoculated leaves of C. amaranticolor, C. quinoa, and N. clevelandii 4 to 6 days post-inoculation. After 10 to 14 days, C. amaranticolor and C. quinoa showed systemic chlorotic spot symptoms, and N. clevelandii, N. benthamiana, and T. repens showed chlorotic spot, mild mosaic, and mosaic in the upper leaves, respectively. Up to now, in South Korea, ClYVV has been detected in gladiolus (Gladiolus gandavensis) (3) and soybean (Glycine max) (4). ClYVV can be easily transmitted by insect, aphid, or mechanical inoculation and has a host range including tobacco, soybean, etc. The presence of ClYVV could become an important threat to crop production in South Korea. To our knowledge, this is the first report of a ClYVV infection of the white clover plant in South Korea. References: (1) B. L. Denny and P. L. Guy. Australas. Plant Pathol. 38:270, 2009. (2) M. Nam et al. Plant Pathol. J. 30:51, 2014. (3) I. S. Park et al. Korean J. Plant Pathol. 14:74, 1998. (4) J. C. Shin et al. Plant Dis. 98:1283, 2014.
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