Dissertations / Theses on the topic 'Protein secretion'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Protein secretion.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Bruns, Caroline 1984. "GRh1-dependent unconventional protein secretion." Doctoral thesis, Universitat Pompeu Fabra, 2013. http://hdl.handle.net/10803/125070.
Full textBesides conventional secretion, in which proteins are transported through the endoplasmic reticulum (ER) and the Golgi complex, unconventional secretion routes bypassing the Golgi complex have been described for different proteins in several organisms. How-ever, the mechanisms of their release remain poorly understood. It was reported that the unconventional secretion of the acyl-CoA binding protein Acb1 from Saccharomyces cerevisiae requires a diverse group of proteins including the GRASP ortholog Grh1, autophagy-related proteins, proteins involved in fusion of membranes with endosomes, members of the ESCRT-machinery, and the plasma membrane t-SNARE Sso1. How these proteins work together for Acb1 secretion remains elusive. Our findings indicate that upon nutrient starvation, the condition known to induce unconventional secretion of Acb1, Grh1 is concentrated in a phosphatidylinositol 3-kinase-dependent manner to unique membrane structures near the ER exit sites. These membranes –shaped like cups– are enriched in PI(3)P and contain the ESCRT-I protein Vps23 as well as the autophagy-related proteins Atg8 and Atg9 thereby bringing together different proteins required for Acb1 secretion. We have named these structures CUPS (Compartment for Unconventional Protein Secretion), based on their shape and content. CUPS, we propose, are the starting point for the formation of Acb1-containing vesicular intermediates dedicated for unconventional secretion.
Finnie, Christine. "Protein secretion by Rhizobium leguminosarum." Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361420.
Full textPayne, Thomas. "Protein secretion in Saccharomyces cerevisiae." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.438772.
Full textDe, Obeso Fernandez Del Valle Alvaro. "Protein secretion and encystation in Acanthamoeba." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31483.
Full textGarrison, Jennifer L. "Small molecule modulation of protein secretion." Diss., Search in ProQuest Dissertations & Theses. UC Only, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3261264.
Full textRavindran, Sandeep. "Effector protein secretion by toxoplasma gondii /." May be available electronically:, 2009. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.
Full textJohnson, Conkright Juliana j. "SORTING AND SECRETION OF SURFACTANT PROTEIN C." University of Cincinnati / OhioLINK, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=ucin990723467.
Full textAhmad, Asma. "Protein-protein interactions in the bacterial type VI secretion system." Thesis, University of Sheffield, 2013. http://etheses.whiterose.ac.uk/4811/.
Full textFu, Zhibiao. "Studies of protein secretion in escherichia coli /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BICH%202006%20FU.
Full textFitchen, Nicola. "Protein localisation and secretion in Helicobacter pylori." Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273120.
Full textWilson, Michael P. "Protein secretion and quorum sensing in Salmonella." Thesis, University of Newcastle Upon Tyne, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275515.
Full textSrinivasan, Supriya. "Developmentally regulated protein secretion in Dictyostelium discoideum /." free to MU campus, to others for purchase, 2000. http://wwwlib.umi.com/cr/mo/fullcit?p9999318.
Full textKrehenbrink, Martin. "Protein secretion in Rhizobium leguminosarum biovar viciae 3841." Thesis, University of East Anglia, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.432434.
Full textAli, Tehmeena. "Characterisation of protein secretion systems of Actinobacillus pleuropneumoniae." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440122.
Full textMoss, Catherine Elizabeth. "G-protein coupled receptors modulating incretin hormone secretion." Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648611.
Full textPalomäki, Tiina. "Identification of a secretion signal for the type II protein secretion pathway in Erwinia carotovora." Helsinki : University of Helsinki, 2003. http://ethesis.helsinki.fi/julkaisut/mat/bioti/vk/palomaki/.
Full textNash, Kevin T. "KISS1 matastasis suppressor secretion is required for metastasis suppression." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2006. https://www.mhsl.uab.edu/dt/2008r/nash.pdf.
Full textTan, Yu Pei. "The development of Lactococcus lactis as an antimicrobial agent." Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/39143/1/Yu_Pei_Tan_Thesis.pdf.
Full textHolland, Alexandria. "Optimisation of feedstock utilisation by Geobacillus thermoglucosidasius." Thesis, University of Bath, 2017. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.723323.
Full textKeller, Martin. "The inflammasome : a key regulator of unconventional protein secretion /." Zürich : ETH, 2008. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17640.
Full textGill, Bhupinder. "Approaches to the study of protein secretion in yeast." Thesis, University of Leicester, 1997. http://hdl.handle.net/2381/30352.
Full textTian, Ya-Min. "The involvement of protein kinase C in insulin secretion." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337551.
Full textColley, Alan D. "Studies of Golgi organization and protein secretion in yeasts." Thesis, University of Edinburgh, 1995. http://hdl.handle.net/1842/19639.
Full textKouwen, Thijs R. H. M. "Protein secretion and disulfide bond handling in bacillus subtilis." [S.l. : [Groningen : s.n.] ; University Library Groningen] [Host], 2009. http://irs.ub.rug.nl/ppn/315686960.
Full textGokoo, Suzanne. "Secretion of GBP, an infective stage-specific protein of Leishmania major." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265838.
Full textMilward, Kelly. "Molecular studies using the Aspergillus nidulans #alpha#-COP homologue." Thesis, Bangor University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367360.
Full textStafford, William Herbert Lee. "Analysis of the merozoite surface protein-1 complex and protein secretion in plasmodium falciparum." Thesis, University College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338464.
Full textAltman, Elliot Emr Scott. "Characterization of the SecB protein, a chaperone that facilitates protein secretion in Escherichia coli /." Diss., Pasadena, Calif. : California Institute of Technology, 1991. http://resolver.caltech.edu/CaltechETD:etd-06152007-080238.
Full textPryde, James Grant. "Biogenesis of secretory granules in the bovine adrenal medulla." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/24238.
Full textSutherland, J. L. "Studies on the patterns of secretion of extracellular proteins by Staphylococcus aureus." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380019.
Full textThwaite, Joanne E. "Factors influencing the production of Bacillus anthracis protective antigen in Bacillus subtilis." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369784.
Full textHousby, J. Nicholas. "The isolation and characterisation of conditional (Out's) and null (Out'-) secretion mutants of Erwinia carotovora subspecies carotovora (SCRI193)." Thesis, University of Warwick, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387335.
Full textSmith, Kenneth. "A conserved Inner Membrane Protein of Aggregatibacter actinomycetemcomitans is integral for membrane function." ScholarWorks @ UVM, 2015. http://scholarworks.uvm.edu/graddis/417.
Full textKornacker, Michael Gilbert. "Analysis of pullulanase secretion from Klebsiella pneumoniae strain K21." Thesis, University of Leicester, 1988. http://hdl.handle.net/2381/34437.
Full textEntwistle, Joanna. "Isolation of genes involved in protein secretion in Aspergillus niger." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.311669.
Full textHuppert, Laura Ann. "Characterization of the Bacillus subtilis ESX-Type Protein Secretion System." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:15821590.
Full textYan, J. "Mass spectrometric studies of proteins and protein complexes involved in bacterial secretion and regulatory systems." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1426517/.
Full textMaldonado, Rodrigo. "Erythropoietin provides a molecular model for protein burden during intracellular processing and secretion of recombinant proteins." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/erythropoietin-provides-a-molecular-model-for-protein-burden-during-intracellular-processing-and-secretion-of-recombinant-proteins(79ca36c2-75f2-4426-8ab9-d74994fa8ed9).html.
Full textAlzahrani, Ashwag. "Identification of Human Proteins Interacting with the Protein IcsB of Shigella flexneri." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38333.
Full textWei, Peter. "The structural characterization of the Saccharomyces cerevisiae alpha mating factor secretion signal for recombinant protein secretion in Pichia pastoris." Scholarly Commons, 2015. https://scholarlycommons.pacific.edu/uop_etds/177.
Full textGhanem, Simona S. "A Role for CEACAM2 protein in Insulin Secretion, Clearance and Action." University of Toledo Health Science Campus / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=mco1491497714470047.
Full textSibanda, Ntsako. "Evaluation of high recombinant protein secretion phenotype of saccharomyces cerevisiae segregant." Thesis, University of Limpopo, 2016. http://hdl.handle.net/10386/1803.
Full textThe ever increasing cost of fossil-based fuels and the accompanying concerns about their impact on the environment is driving research towards clean and renewable sources of energy. Bioethanol has the potential to be a replacement for liquid transportation fuels. In addition to its near zero nett carbon dioxide emissions, bio-ethanol has a high energy to weight ratio and can easily be stored in high volumes. To produce bioethanol at economically competitive prices, the major cost in the production process needs to be addressed. The addition of enzymes to hydrolyse the lignocellulosic fraction of the agricultural waste to simple sugars is considered to be the major contributor to high production cost. A consolidated bioprocess (CBP) which ideally combines all the steps that are currently accomplished in different reactors by different microorganisms into a single process step would be a more economically feasible solution. In this study the potential of yeast hybridization with a CBP approach was used. In order to evaluate the reduction or elimination of the addition of cellulolytic and hemi-cellulolytic enzymes to the ethanol production process. High cellobiohydrolase I secreting progeny from hybridization of an industrial bioethanol yeast strain, S. cerevisiae M0341, and a laboratory strain S. cerevisiae Y294 were isolated. In order to determine if this characteristic was specific to cellobiohydrolase I secretion, these strains were evaluated for their ability to secrete other relevant recombinant hydrolase enzymes for CBP-based ethanol production. A total of seven S. cerevisiae strains were chosen from a progeny pool of 28 supersecreting hybrids and reconstructed to create two parental strains; S. cerevisiae M0341 and S. cerevisiae Y294, together with their hybrid segregants strains H3M1, H3M28, H3H29, H3K27 and H3O23. Three episomal plasmids namely pNS201, pNS202 and pNS203 were constructed; these plasmids together with two already available plasmids, namely pRDH166 and pRDH182 contained genes for different reporter enzymes, namely β-glucosidase I, xylanase II, endoglucanase lll, cellobiohydrolase l and α-glucuronidase. To allow for selection of the episomal plasmids, homologous recombination was used to replace the functional URA3 gene of selected strains, with the non-functional ura3 allele from the Y294 strain. Enzyme activity was used as an indicator of the amount of enzyme secreted. Fermentation studies in a bioreactor were used to determine the metabolic burden imposed on the segregants expressing the cellobiohydrolase at high levels. In addition all segregants were tested for resistance to inhibitors commonly found in pre-treated lignocellulosic material. The M28_Cel7A was found to be the best secretor of Cel7A (Cellobiohydrolase l); however it seems as though this phenomenon imposes a significant metabolic burden on the yeast. The supersecreting hybrid strains cannot tolerate lignocellulosic inhibitors at concentrations commonly produced during pretreatment
The National Research Foundation - Renewable Energy Scholarship (NRF-RSES)
Komar, Joanna. "Activity of the holo-translocon and its individual components in protein secretion and membrane protein insertion." Thesis, University of Bristol, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.702120.
Full textUpritchard, Hamish Graeme, and n/a. "Host interactions with Pseudomonas aeruginosa : a proteomic approach." University of Otago. Department of Biochemistry, 2005. http://adt.otago.ac.nz./public/adt-NZDU20060804.101030.
Full textLi, Zhiguo. "Structure, secretion, and proteolysis study of MBP-containing heterologous proteins in Pichia pastoris." Scholarly Commons, 2010. https://scholarlycommons.pacific.edu/uop_etds/2415.
Full textGuo, Dongni Lily Centre for Vascular Research Faculty of Medicine UNSW. "Protein kinase A and related pathways in the regulation of apolipoprotein E secretion and catalase activity." Awarded By:University of New South Wales. Centre for Vascular Research, 2009. http://handle.unsw.edu.au/1959.4/41512.
Full textChen, Chen. "Studies on Selective Protein Loading onto Extracellular Membrane Vesicles of a Novel Cold-Adapted Bacterium, Shewanella vesiculosa HM13." Kyoto University, 2020. http://hdl.handle.net/2433/253331.
Full text0048
新制・課程博士
博士(農学)
甲第22495号
農博第2399号
新制||農||1076(附属図書館)
学位論文||R2||N5275(農学部図書室)
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 栗原 達夫, 教授 小川 順, 教授 木岡 紀幸
学位規則第4条第1項該当
Miller, Paul E. "Differential secretion from prestored heterogeneous protein sources is the basis of regulated nonparallel digestive enzyme secretion by the exocrine pancreas." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=74316.
Full textKriel, Johan Hendrik. "Development of synthetic signal sequences for heterologous protein secretion from Saccharomyces cerevisiae." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53364.
Full textENGLISH ABSTRACT: Protein secretion and intracellular transport are highly regulated processes and involve the interplay of a multitude of proteins. A unique collection of thermosensitive secretory mutants allowed scientists to demonstrate that the secretory pathway of the yeast Saccharomyces cerevisiae is very similar to that of the higher eukaryotes. All proteins commence their journey in the endoplasmic reticulum, where they undergo amino-linked core glycosyl modification. After passage through the Golgi apparatus, where the remodelling of the glycosyl chains is completed, proteins are transported to their final destinations, which are either the cell surface, periplasmic space or the vacuole. Proteins destined for secretion are usually synthesised with a transient amino-terminal secretion leader of varying length and hydrophobicity, which plays a crucial role in the targeting and translocation of their protein cargo. Considerable effort has been made to elucidate the molecular mechanisms involved in these processes, especially due to their relevance in a rapidly expanding biotech industry. The advantages of S. cerevisiae as a host for the expression of recombinant proteins are well documented. Unfortunately, S. cerevisiae is also subject to a number of drawbacks, with a relative low product yield being one of the major disadvantages. Bearing this in mind, different secretion leaders were compared with the aim of improving the secretion of the LKA 1 and LKA2 a-amylase enzymes from the S. cerevisiae secretion system. The yeast Lipomyces kononenkoae is well known for its ability to degrade raw starch and an improved secretion of its amylase enzymes from S. cerevisiae paves the way for a potential one-step starch utilisation process. Three sets of constructs were prepared containing the LKA 1 and LKA2 genes separately under secretory direction of either their native secretion leader, the S. cerevisiae mating pheromone a-factor (MFa1) secretion leader, or the MFa1 secretion leader containing a synthetic C-terminal spacer peptide (EEGEPK). The inclusion of a spacer peptide in the latter set of constructs ensured improved Kex2p proteolytic processing of the leader/protein fusion. Strains expressing the amylase genes under their native secretion leaders resulted in the highest saccharolytic activity in the culture medium. In contrast to this, strains utilising the synthetic secretion leader produced the highest fermentation yield, but had a lower than expected extracellular activity. We hypothesise that the native amylase leaders may function as intramolecular chaperones in the folding and processing of their passenger proteins, thereby increasing processing efficiency and concomitant enzyme activity.
AFRIKAANSE OPSOMMING: Proteïensekresie en intrasellulêre transport is hoogs gereguleerde prosesse en betrek die onderlinge wisselwerking van 'n verskeidenheid proteïene. 'n Unieke versameling van temperatuur-sensitiewe sekresiemutante het wetenskaplikes in staat gestelom die ooreenkoms tussen die sekresiepad van die gis Saccharomyces cerevisiae en dié van komplekser eukariote aan te toon. Alle proteïene begin hul reis in die endoplasmiese retikulum, waartydens hulle ook amino-gekoppelde kernglikosielveranderings ondergaan. Nadat die proteïene deur die Golgi-apparaat beweeg het, waar die laaste veranderings aan die glikosielkettings plaasvind, word hulle na hul finale bestemmings, waaronder die seloppervlak, die periplasmiese ruimte of die vakuool, vervoer. Proteïene wat vir sekresie bestem is, word gewoonlik met 'n tydelike, amino-eindpuntsekresiesein, wat 'n kritiese rol in die teiken en translokasie van hul proteïenvrag speel, gesintetiseer. Heelwat pogings is in hierdie studie aangewend om die molekulêre meganismes betrokke by hierdie prosesse te ontrafel, veral as gevolg van hul toepaslikheid in 'n vinnig groeiende biotegnologiebedryf. Die voordele van S. cerevisiae as 'n gasheer vir die uitdruk van rekombinante proteïene is alombekend. S. cerevisiae het egter ook verskeie nadele, waaronder die relatiewe lae produkopbrengs die belangrikste is. Teen hierdie agtergrond, is verskillende sekresieseine met mekaar vergelyk met die doelom die sekresie van die LKA 1 en LKA2 a-amilasegene vanuit die S. cerevisiae-uitdrukkingsisteem te verbeter. Die gis Lipomyces kononenkoae is bekend vir sy vermoeë om rou stysel af te breek en 'n verbeterde sekresie van sy amilasegene vanuit S. cerevisiae baan die weg vir 'n moontlike een-stap styselgebruiksproses. Drie stelle konstrukte is gemaak wat die LKA 1- en LKA2- gene onafhanklik onder sekresiebeheer van onderskeidelik hul inheemse sekresiesein, die S. cerevisiae paringsferomoonsekresiesein (MFa1) of die MFa1-sekresiesein met 'n sintetiese koppelingspeptied aan die C-eindpunt (EEGEPK), plaas. Die insluiting van 'n koppelingspeptied in die laasgenoemde stel konstrukte verseker verbeterde Kex2p proteolitiese prosessering van die sein/proteïenfusie. Rasse wat die amilasegene onder beheer van hul inheemse sekresieseine uitdruk, het die beste saccharolitiese aktiwiteit in die kultuurmedia getoon. In teenstelling hiermee, het rasse wat van die sintetiese sekresiesein gebruik maak, die beste fermentasie-opbrengs getoon, maar met 'n laer as verwagte ekstrasellulêre aktiwiteit. Ons vermoed dat die inheemse amilaseseine as intramolekulêre begeleiers optree in die vou en prosessering van hul proteïenpassasiers, wat lei tot verbeterde prosessering en ensiemaktiwiteit.
Creasey, Elizabeth Anne. "Protein interactions in the Type III secretion system of Enteropathogenic Escherichia Coli." Thesis, Imperial College London, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.410342.
Full text