Relevant bibliographies by topics / Protein based delivery systems

Academic literature on the topic 'Protein based delivery systems'

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Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Protein based delivery systems"

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Hong, Seyoung, Dong Wook Choi, Hong Nam Kim, Chun Gwon Park, Wonhwa Lee, and Hee Ho Park. "Protein-Based Nanoparticles as Drug Delivery Systems." Pharmaceutics 12, no. 7 (June 29, 2020): 604. http://dx.doi.org/10.3390/pharmaceutics12070604.

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Nanoparticles have been extensively used as carriers for the delivery of chemicals and biomolecular drugs, such as anticancer drugs and therapeutic proteins. Natural biomolecules, such as proteins, are an attractive alternative to synthetic polymers commonly used in nanoparticle formulation because of their safety. In general, protein nanoparticles offer many advantages, such as biocompatibility and biodegradability. Moreover, the preparation of protein nanoparticles and the corresponding encapsulation process involved mild conditions without the use of toxic chemicals or organic solvents. Protein nanoparticles can be generated using proteins, such as fibroins, albumin, gelatin, gliadine, legumin, 30Kc19, lipoprotein, and ferritin proteins, and are prepared through emulsion, electrospray, and desolvation methods. This review introduces the proteins used and methods used in generating protein nanoparticles and compares the corresponding advantages and disadvantages of each.
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Dahiya, Sunita, and Rajiv Dahiya. "BIOAVAILABILITY ENHANCEMENT AND LIPID NANOCARRIER BASED DELIVERY OF PEPTIDES AND PROTEINS." Bulletin of Pharmaceutical Research 10, no. 1-3 (2020): 1–10. http://dx.doi.org/10.21276/bpr.2020.10.3.

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Peptides and proteins are vital biomacromolecules that perform several bodily functions in various physiological and biological processes. Being biocompatible and biodegradable, these macromolecules are considered promising platforms for delivery of drugs and genes. However, peptides and proteins suffer from major limitations including enzymatic degradation, short circulation half-lives, and poor membrane permeability that leads to poor bioavailability, challenging their effective delivery. This article briefly discusses the inherent challenges in peptide and protein delivery along with strategies for bioavailability enhancement and lipid nanocarriers as prospective systems for peptide and protein drug delivery.
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Numata, Keiji, Balajikarthick Subramanian, Heather A. Currie, and David L. Kaplan. "Bioengineered silk protein-based gene delivery systems." Biomaterials 30, no. 29 (October 2009): 5775–84. http://dx.doi.org/10.1016/j.biomaterials.2009.06.028.

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Chen, Yingzhi, Meng Zhang, Kyoung Ah Min, Huiyuan Wang, Meong Cheol Shin, Feng Li, Victor C. Yang, and Yongzhuo Huang. "Improved Protein Toxin Delivery Based on ATTEMPTS Systems." Current Drug Targets 19, no. 4 (February 19, 2018): 380–92. http://dx.doi.org/10.2174/1389450118666170302094758.

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Chen, Lingyun, Gabriel E. Remondetto, and Muriel Subirade. "Food protein-based materials as nutraceutical delivery systems." Trends in Food Science & Technology 17, no. 5 (May 2006): 272–83. http://dx.doi.org/10.1016/j.tifs.2005.12.011.

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Berillo, Dmitriy, Adilkhan Yeskendir, Zharylkasyn Zharkinbekov, Kamila Raziyeva, and Arman Saparov. "Peptide-Based Drug Delivery Systems." Medicina 57, no. 11 (November 5, 2021): 1209. http://dx.doi.org/10.3390/medicina57111209.

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Peptide-based drug delivery systems have many advantages when compared to synthetic systems in that they have better biocompatibility, biochemical and biophysical properties, lack of toxicity, controlled molecular weight via solid phase synthesis and purification. Lysosomes, solid lipid nanoparticles, dendrimers, polymeric micelles can be applied by intravenous administration, however they are of artificial nature and thus may induce side effects and possess lack of ability to penetrate the blood-brain barrier. An analysis of nontoxic drug delivery systems and an establishment of prospective trends in the development of drug delivery systems was needed. This review paper summarizes data, mainly from the past 5 years, devoted to the use of peptide-based carriers for delivery of various toxic drugs, mostly anticancer or drugs with limiting bioavailability. Peptide-based drug delivery platforms are utilized as peptide–drug conjugates, injectable biodegradable particles and depots for delivering small molecule pharmaceutical substances (500 Da) and therapeutic proteins. Controlled drug delivery systems that can effectively deliver anticancer and peptide-based drugs leading to accelerated recovery without significant side effects are discussed. Moreover, cell penetrating peptides and their molecular mechanisms as targeting peptides, as well as stimuli responsive (enzyme-responsive and pH-responsive) peptides and peptide-based self-assembly scaffolds are also reviewed.
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Guan, Tongwei, Zhiheng Zhang, Xiaojing Li, Shaoning Cui, David Julian McClements, Xiaotian Wu, Long Chen, et al. "Preparation, Characteristics, and Advantages of Plant Protein-Based Bioactive Molecule Delivery Systems." Foods 11, no. 11 (May 26, 2022): 1562. http://dx.doi.org/10.3390/foods11111562.

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As a renewable resource, the market trend of plant protein has increased significantly in recent years. Compared with animal protein, plant protein production has strong sustainability factors and a lower environmental impact. Many bioactive substances have poor stability, and poor absorption effects limit their application in food. Plant protein-based carriers could improve the water solubility, stability, and bioavailability of bioactive substances by different types of delivery systems. In this review, we present a detailed and concise summary of the effects and advantages of various plant protein-based carriers in the encapsulation, protection, and delivery of bioactive substances. Furthermore, the research progress of food-grade bioactive ingredient delivery systems based on plant protein preparation in recent years is summarized, and some current challenges and future research priorities are highlighted. There are some key findings and conclusions: (i) plant proteins have numerous functions: as carriers for transportation systems, a shell or core of a system, or food ingredients; (ii) plant protein-based carriers could improve the water solubility, stability, and bioavailability of bioactive substances by different types of delivery systems; and (iii) plant protein-based carriers stabilize bioactive substances with potential applications in the food and nutrition fields.
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Amidi, Maryam, Enrico Mastrobattista, Wim Jiskoot, and Wim E. Hennink. "Chitosan-based delivery systems for protein therapeutics and antigens." Advanced Drug Delivery Reviews 62, no. 1 (January 2010): 59–82. http://dx.doi.org/10.1016/j.addr.2009.11.009.

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Chatterjee, Shayeri, Beduin Mahanti, Subhabrota Majumdar, and Rana Mazumder. "APPROACHES AND ROLE OF PROTEIN BASED NANOPARTICLES IN DRUG DELIVERY SYSTEM: A REVIEW." Indian Research Journal of Pharmacy and Science 6, no. 2 (June 2019): 1879–87. http://dx.doi.org/10.21276/irjps.2019.6.2.8.

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Ittig, Simon J., Christoph Schmutz, Christoph A. Kasper, Marlise Amstutz, Alexander Schmidt, Loïc Sauteur, M. Alessandra Vigano, et al. "A bacterial type III secretion-based protein delivery tool for broad applications in cell biology." Journal of Cell Biology 211, no. 4 (November 23, 2015): 913–31. http://dx.doi.org/10.1083/jcb.201502074.

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Methods enabling the delivery of proteins into eukaryotic cells are essential to address protein functions. Here we propose broad applications to cell biology for a protein delivery tool based on bacterial type III secretion (T3S). We show that bacterial, viral, and human proteins, fused to the N-terminal fragment of the Yersinia enterocolitica T3S substrate YopE, are effectively delivered into target cells in a fast and controllable manner via the injectisome of extracellular bacteria. This method enables functional interaction studies by the simultaneous injection of multiple proteins and allows the targeting of proteins to different subcellular locations by use of nanobody-fusion proteins. After delivery, proteins can be freed from the YopE fragment by a T3S-translocated viral protease or fusion to ubiquitin and cleavage by endogenous ubiquitin proteases. Finally, we show that this delivery tool is suitable to inject proteins in living animals and combine it with phosphoproteomics to characterize the systems-level impact of proapoptotic human truncated BID on the cellular network.
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Dissertations / Theses on the topic "Protein based delivery systems"

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Squire, Marie A. "Protein-based drug delivery systems." Thesis, University of Canterbury. Chemistry, 2004. http://hdl.handle.net/10092/6518.

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The targeted delivery of drugs is one of the most actively pursued goals in anti-HIV and anti-cancer chemotherapy. This project takes a proof-of-concept approach to the development of protein-based drug delivery systems - delivery systems that would package, target, and deliver cytotoxins to diseased cells. Primarily, this project explores the use of the potent anti-HN protein, cyanovirin-N (CV-N), to actively target and deliver cytotoxic natural products to HN-infected cells. This project also investigates the use of human serum albumin (HSA), a 66 kDa protein, as a macromolecular carrier to passively target and deliver cytotoxic natural products to cancerous cells. To facilitate release of the toxin within infected cells, an enzymatically-cleavable tetra peptide was incorporated in the conjugates. Maleimido-activated tetra peptide toxin constructs were prepared in readiness for selective reaction with proteins carrying thiol functionalities. Release of the toxin, norhomohalichondrin B, was demonstrated in vitro. Native CV -N conjugates were prepared by thiolation of the lysine ε-amino groups, and the subsequent reaction with maleimido-activated compounds. Reaction across all lysine residues was demonstrated. A singly-substituted tyrosinamide conjugate of CV-N was prepared. Two recombinantly produced mutant CV-N proteins allowed for the production of selectively modified, double- and single-norhomohalichondrin B conjugates of CV-N. The conjugates retained the anti-HN activity of the parent protein. Homohalichondrin B, doxorubicin, and tyrosinamide conjugates of HSA were prepared. The syntheses exploited the availability of a free thiolmoiety at cysteine-34 of HSA, and the specific and selective reaction of this thiol with the maleimido-activated tetra peptide derivatives. All toxin conjugates demonstrate excellent cell toxicity. Further research to investigate whether this is targeted toxicity is currently underway.
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Jørgensen, Lene. "Lipid based drug delivery systems for parenteral delivery of proteins /." Cph. : Department of Pharmaceutics, the Danish University of Pharmaceutical Sciences, 2004. http://www.dfh.dk/phd/defences/lenejoergensen.htm.

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Konovalova, M. V., D. S. Khramova, A. V. Ilyina, and D. V. Kurek. "Polysaccharides Based Nanoparticles as Protein Oral Delivery System." Thesis, Sumy State University, 2013. http://essuir.sumdu.edu.ua/handle/123456789/35483.

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Ovalbumin loaded chitosan and pectin based composite nanoparticles were obtained. The mean size, morphology and zeta potential of the nanoparticles were determined by dynamic light scattering (DLS) and atomic force microscopy (AFM). Effect oral administration of obtained nanoparticles on the immune response was studied. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/35483
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Schwab, Martin. "Degradation of lipid based drug delivery systems and characterization of semi-synthetic spider silk proteins for the application in pharmaceutical technology." Diss., Ludwig-Maximilians-Universität München, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-165238.

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Schwab, Martin [Verfasser], and Gerhard [Akademischer Betreuer] Winter. "Degradation of lipid based drug delivery systems and characterization of semi-synthetic spider silk proteins for the application in pharmaceutical technology / Martin Schwab. Betreuer: Gerhard Winter." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2014. http://d-nb.info/1046785257/34.

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游, 晧欣. "Development of novel systems promoting intracellular protein delivery." 京都大学 (Kyoto University), 2012. http://hdl.handle.net/2433/157905.

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Heffernan, Michael John. "Biodegradable polymeric delivery systems for protein subunit vaccines." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/24787.

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Thesis (Ph.D.)--Biomedical Engineering, Georgia Institute of Technology, 2008.
Committee Chair: Dr. Niren Murthy; Committee Member: Dr. Carson Meredith; Committee Member: Dr. Julia Babensee; Committee Member: Dr. Mark Prausnitz; Committee Member: Dr. Ravi Bellamkonda.
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Pape, Valerie Elizabeth. "Methotrexate-protein conjugates as soluble drug delivery systems." Thesis, University of Bath, 1990. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.277877.

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Liu, Weipeng. "Biopolymer-based ocular drug delivery systems." Diss., Connect to online resource - MSU authorized users, 2008.

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Estey, Tia Brie. "Protein instability associated with PLGA delivery systems and UV-induced protein oxidation /." Connect to full text via ProQuest. IP filtered, 2006.

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Thesis (Ph.D. in Pharmaceutical Sciences) -- University of Colorado, 2006.
Typescript. Includes bibliographical references (leaves 144-161). Free to UCDHSC affiliates. Online version available via ProQuest Digital Dissertations;
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Books on the topic "Protein based delivery systems"

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1961-, McNally Eugene J., ed. Protein formulation and delivery. New York: M. Dekker, 2000.

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Bari, Elia, Sara Perteghella, and Maria Luisa Torre, eds. Silk-based Drug Delivery Systems. Cambridge: Royal Society of Chemistry, 2020. http://dx.doi.org/10.1039/9781839162664.

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Cheng, Yiyun, ed. Dendrimer-Based Drug Delivery Systems. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9781118275238.

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Lu, Wan-Liang, and Xian-Rong Qi, eds. Liposome-Based Drug Delivery Systems. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-49231-4.

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1956-, Svenson Sönke, American Chemical Society. Division of Colloid and Surface Chemistry, and American Chemical Society Meeting, eds. Carrier-based drug delivery. Washington, D.C: American Chemical Society, 2004.

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Smadar, Cohen, and Bernstein Howard, eds. Microparticulate systems for the delivery of proteins and vaccines. New York: Marcel Dekker, 1996.

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Nayak, Amit Kumar, Md Saquib Hasnain, and Dilipkumar Pal, eds. Ionically Gelled Biopolysaccharide Based Systems in Drug Delivery. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-2271-7.

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Desai, Murli. Rights-based Integrated Child Protection Service Delivery Systems. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-13-8534-6.

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NATO Advanced Research Workshop on Advanced Drug Delivery Systems for Peptides and Proteins (1986 Copenhagen, Denmark). Delivery systems for peptide drugs. New York: Plenum Press, 1986.

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Dendrimer-based drug delivery systems: From theory to practice. Hoboken, N.J: John Wiley & Sons, 2012.

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Book chapters on the topic "Protein based delivery systems"

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Ding, Dan, and Xiqun Jiang. "Drug Delivery from Protein-Based Nanoparticles." In Bioinspired and Biomimetic Polymer Systems for Drug and Gene Delivery, 149–70. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2014. http://dx.doi.org/10.1002/9783527672752.ch6.

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Soonpaa, Mark H., and Loren J. Field. "Cell-Based Myocardial Protein Delivery." In Gene Transfer in the Cardiovascular System, 333–53. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-6277-1_15.

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Mansor, Muhammad Haji, Emmanuel Garcion, Bathabile Ramalapa, Nela Buchtova, Clement Toullec, Marique Aucamp, Jean Le Bideau, et al. "Polymer-Based Protein Delivery Systems for Loco-Regional Administration." In Green Synthesis in Nanomedicine and Human Health, 249–70. First edition. | Boca Raton, FL : CRC Press, [2021]: CRC Press, 2021. http://dx.doi.org/10.1201/9781003023197-17.

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Prokop, Ales, Evgenii Kozlov, Gianluca Carlesso, and Jeffrey M. Davidson. "Hydrogel-Based Colloidal Polymeric System for Protein and Drug Delivery: Physical and Chemical Characterization, Permeability Control and Applications." In Filled Elastomers Drug Delivery Systems, 119–73. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/3-540-45362-8_3.

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Fonte, Pedro, José Carlos Andrade, Vítor Seabra, and Bruno Sarmento. "Chitosan-Based Nanoparticles as Delivery Systems of Therapeutic Proteins." In Methods in Molecular Biology, 471–87. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-921-1_28.

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Dhanasooraj, Dhananjayan, R. Ajay Kumar, and Sathish Mundayoor. "Subunit Protein Vaccine Delivery System for Tuberculosis Based on Hepatitis B Virus Core VLP (HBc-VLP) Particles." In Vaccine Design, 377–92. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3389-1_26.

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Niazi, Sarfaraz K. "Therapeutic Protein Delivery Systems." In The Future of Pharmaceuticals, 345–80. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003146933-10.

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Shoyele, Sunday A. "Engineering Protein Particles for Pulmonary Drug Delivery." In Drug Delivery Systems, 149–60. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-210-6_7.

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Vannucci, Luca, Elisabetta Falvo, and Pierpaolo Ceci. "Multifunctional Protein-Based Nanoparticles for Cancer Theranosis." In Intracellular Delivery II, 231–53. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-8896-0_12.

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Emtage, Spencer. "Biotechnology and Protein Production." In Delivery Systems for Peptide Drugs, 23–33. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4757-9960-6_2.

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Conference papers on the topic "Protein based delivery systems"

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Blanco, Letia, Panos S. Shiakolas, Pranesh B. Aswath, Christopher B. Alberts, Chris Grace, Kyle Godfrey, and Drew Patin. "A Thermoresponsive Hydrogel Based Controlled Drug Delivery Device." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-88564.

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Thermoresponsive hydrogels exhibit the unique property of volume change as a function of change in temperature as they transition between hydrophilic and hydrophobic states. These hydrogels can be loaded with drug/protein and serve as reservoirs for drug/protein delivery applications. A hydrogel based device for controlled drug delivery is designed with a number of subsystems that are interfaced with LabVIEW for development of a functional device. The device was designed using analytical and finite element analysis procedures and fabricated. In this manuscript, the device design will be reviewed and discussed. A parametric study was performed to examine the device operation and performance as function of hydrogel heating/cooling temperature profiles. Subsequently, the device was employed in a series of experiments to examine the delivery of a protein as a function of thermal stimuli. The matrix used in this study was poly(ethylene glycol) diacrylate (PEGDA) and the drug delivery nanoparticles carriers were poly(N-isopropylacrylamide-co-acrylamide (PNIPAM) with a lower critical solution temperature (LCST) around 40°C. The protein of choice was bovine serum albumin (BSA). The results of this study illustrate that the development of a multi-drug or therapeutic delivery device is possible and that individual drugs can be delivered on demand using a closed loop control system.
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Zheng, Zhuoyuan, Akash Singh, and Yumeng Li. "Molecular Dynamic Simulation Study on Soy Protein As Drug Delivery Vehicle." In ASME 2020 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/imece2020-23590.

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Abstract Protein-based drug carriers are promising candidates for efficient drug delivery among the available potential colloidal carrier systems, due to their low cytotoxicity, abundance, renewability, diverse functional groups and interactions, and high drug loading capacity, etc. In this study, molecular dynamics (MD) simulations are performed to study the mechanisms of 11S molecule of soy protein as drug delivery vehicle to attach allyl isothiocyanate (AITC) and doxorubicin (DOX) drugs. The intermolecular interactions between protein and drugs are investigated; and the loading capacities of the protein molecules are calculated and compared with experiments. It is found that, for the AITC system, both nonpolar and polar residues of protein have the ability to adsorb AITCs; particularly, the polar residues serve as the primary active sites for the stable attachment of the drug molecules through the electrostatic (dipole-dipole) interactions. For the DOX system, however, the main driving force become the π-π stacking (the van der Waals interactions) among the aromatic rings of DOX and protein. In addition to pristine protein, different denaturation processes are found to be able to increase the exposure of active sites, therefore, enhance the loading efficiency of the protein carriers.
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Osaki, Toshihisa, and Shoji Takeuchi. "Dielectrophoresis-based liposome delivery to a planar lipid membrane for efficient membrane protein reconstitution." In 2010 IEEE 23rd International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2010. http://dx.doi.org/10.1109/memsys.2010.5442360.

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Priyoatmojo, Dadang, Tri Handayani, Afi Candra Trinugraha, Teguh Wahyono, and Nina Herlina. "Soy Protein Isolate (SPI) Based Delivery System as Promising Mastitis Vaccine Carrier Candidate." In International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021). Paris, France: Atlantis Press, 2022. http://dx.doi.org/10.2991/absr.k.220309.035.

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Shi, Xiaolei. "3D Printing of Gelatin/Alginate Based Hydrocolloids as Delivery Systems for Food and Pharmaceutical Applications." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/oyjy1031.

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3D printing technology has been applied in bioprinting to fabricate three-dimensional matrices to upload living cells, biomaterials, and active ingredients, thus protecting the encapsulated active compounds. Food-grade, protein-based hydrocolloids such as gelatin, collagen, and carrageenan have been used as bioprinting materials and thickening/gelling agents commonly used in the food industry; however, the research of this area is still in its infancy. The objective of this series of studies was to investigate the feasibility of developing a 3D printed, hydrocolloid-based delivery system for active ingredients in the areas of food and pharmaceutical applications. Hydrogels were prepared using alginate and gelatin (A/G) with total solids (w/w%) of 3%, 5%, and 7% at A/G ratios of 1/2, 1/1, and 2/1. The 3D printability was assessed by flow ramp test and frequency sweep. After 3D printing, freeze-drying was conducted to solidify and dehydrate the hydrogels. Hydrogels with formulations of 3% A/G 1/2, 5% A/G 1/1, and 7% A/G 2/1 demonstrated shear-thinning flow behavior, and viscoelasticity of storage modulus (Gʹ) higher than loss modulus (Gʺ), with a loss factor (tan= Gʺ/Gʹ) in the range of 0.50-0.60 at the frequency sweep of 15-40 rad/s. The freeze-dried matrices demonstrated significantly increased hardness and crunchiness, which indicated that the novel matrix had distinguished texture properties. In one study, Bifidobacterium lactis at 10^11 CFU/g was encapsulated within alginate/gelatin hydrogels, 3D printed into tear-drop shapes, and followed by freeze-drying. The results demonstrated that the probiotics encapsulated in the novel matrix have the potential to maintain > 10^6 CFU/g during an 8-week shelf-life test conducted at ambient temperature. This study validated that this 3D-printed, hydrogel-based matrix has the potential to be used as a convenient, shelf-stable delivery system for active ingredients.
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Kluge, Jonathan A., Rudra A. Pampati, Mara L. Schenker, Daniel J. Zhou, John E. Esterhai, David L. Kaplan, and Robert L. Mauck. "Delivery of Active FGF-2 From Mechanically-Stable Biological Nanofibers Accelerates Cell Ingress Into Multifiber Composites." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53955.

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Fibrocartilaginous tissues such as the meniscus and annulus fibrosus serve critical load-bearing roles, relying on arrays of highly organized collagen fibers to resist tensile loads [1]. As these specialized structures are often injured, there exists great demand for engineered tissues for repair or replacement. Cell-laden aligned nanofibrous scaffolds formed from poly(ε-caprolactone) (PCL) have shown promise in achieving tissuelike mechanical and biochemical properties and can direct cellular and matrix organization in vitro [2]. A current limitation of nanofibrous scaffolds, however, is a slow rate of cellular infiltration, particularly in thick scaffolds. To address this, dynamic composite nanofibrous scaffolds have been fabricated via multi-fiber spinning [3], which can offer tunable modes of degradation depending on the polymer sources. For example, water-soluble polyethylene oxide (PEO) fibers can be co-spun with PCL to improve porosity and hasten cell ingress [4]. Incorporation of additional tunable and bioactive polymer sources may add greater versatility to these composite systems. For example, aqueous-based silk fibroin can be used as a slow-degrading, mechanically strong composite fiber component [5] into which active biologic factors (drugs, growth factors) can be incorporated [6]. Variably-degradable silk fibers can be formed by modulating post-spinning treatments, and protein release kinetics can likewise be manipulated by the physical crosslinking method [7]. We hypothesized that incorporation of robust and tunable silk protein-based fibers into a composite of slow-degrading synthetic fibers would provide mechanical function while delivering active biologic factors to expedite cell proliferation and encourage more rapid construct colonization. To test this hypothesis, we characterized the release kinetics of recombinant FGF-2 from silk fibers and its bioactivity in vitro and in a rat subcutaneous implant model.
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Matveev, Konstantin I., Thomas T. Goodman, Jingyang Chen, and Suzie H. Pun. "Parametric Modeling Study of Nanoparticle Penetration Into Spherical Cell Clusters." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-41153.

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Nanoparticle-based drug delivery is a promising cancer treatment method due to the ability to target tumor sites by preferential extravasation and to deliver higher loads of therapeutics. Although nanoparticle penetration in tumor tissue is limited due to diffusional restrictions, delivery can be improved by enzymatic degradation of extracellular matrix proteins at the tumor site. Here, a mathematical model describing transport of nanoparticles in non-uniformly porous spheroids is developed, accounting for binding of particles with cells and endocytosis. Results of parametric simulations for nanoparticle concentration inside spheroids highlight the influence of various system parameters. Preliminary experimental data show qualitative agreement with the theory. These results are useful for understanding nanoparticle delivery and for designing drug delivery strategies.
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Nan, Xing, Tian Feng, Shengjun Liu, Hong Liu, Changjun Liu, Jian Yang, and Chunnian He. "Chitosan Polyelectrolyte Nanoparticles As Protein Delivery Systems." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2009). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5162501.

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Abioye, Raliat, Caleb Acquah, Chibuike Udenigwe, Nico Huttmann, and Pei Chun Queenie Hsu. "Self-assembly and hydrogelation properties of egg white-derived peptides." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/jzku2300.

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Functional foods are gaining traction as a source of peptides possessing hydrogelation properties. Analysis of peptides (n=429) in egg white protein hydrolysates resulted in the identification of six peptides: IFYCPIAIM, NIFYCPIAIM, VLVNAIVFKGL, YCPIAIMSA, MMYQIGLF, and VYSFSLASRL as prominent self-assembly candidates based on prediction of their aggregation-prone segments. The objective of this study was to characterize the hydrogel formed via self-assembly of the peptides. Of the six peptides studied, NIFYCPIAIM and MMYQIGLF showed promising self-assembly and hydrogelation properties. Thioflavin T kinetics indicated that NIFYCPIAIM possesses the strongest self-assembly property, confirmed by dynamic light scattering which indicated the largest average particle diameter was achieved after 24 hours. Rheological characterization indicated that all six peptides possessed viscoelastic pseudoplastic properties and some were able to regain some level of viscosity following the exertion of shear stress. Finally, transmission electron microscopy of the six peptides showed the development of fibrillar structures of varying morphologies after 24 hours. The remarkable difference in self-assembly and hydrogelation properties of NIFYCPIAIM, IFYCPIAIMSA and YCPIAIMSA, which share a common sequence YCPIAIM, indicate the importance of amino acid sequence in the formation and property of peptide hydrogels. Identification of the egg white-derived peptides with hydrogelation properties shows a promising future for the use of functional foods in applications of drug delivery systems and tissue engineering, in the food, pharmaceutical, cosmetics, and biomedical sectors.
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Wei, Chen, Shen Ruili, Cheng Xiang, Wan Tianbao, Zhao Jun, and Zhou Zhubing. "Design and Dehumidification Effect of Dry Air Dehumidification System Inside the Main Cable." In IABSE Congress, Nanjing 2022: Bridges and Structures: Connection, Integration and Harmonisation. Zurich, Switzerland: International Association for Bridge and Structural Engineering (IABSE), 2022. http://dx.doi.org/10.2749/nanjing.2022.1677.

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<p>To improve the durability of the main cable of the suspension bridge, a new dehumidification system for delivering dry air from the interior of the main cable is proposed. The dehumidification system delivers dry air through the dry air supply conduct embedded inside the main cable, which can improve the dry air delivery efficiency. Based on the 1560m long-span suspension bridge of Longtan Yangtze River Bridge, the overall design of the new dehumidification system of the main cable is carried out. The components of the new dehumidification system are designed, the key technologies for the design of the new dehumidification system are clarified, and the corresponding solutions are proposed. To verify the dehumidification effect of the new dehumidification system, the main cable dehumidification test was carried out to test the relative humidity change pattern inside the main cable during the dry air delivering process. The test results show that the new dehumidification system has a good dehumidification effect and can be used to protect the main cable of the long-span suspension bridge against corrosion.</p>
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Reports on the topic "Protein based delivery systems"

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Sampson, James, Jill Lumsden, Darrin Carr, and Elisa Rudd. A Differential Feature-Cost Analysis of Internet-Based Career Information Delivery Systems (CIDS). Florida State University Libraries, December 1999. http://dx.doi.org/10.17125/fsu.1525966258.

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Popova, Teodora, Borislav Tzankov, Christina Voycheva, Krassimira Yoncheva, and Nikolai Lambov. Development of Advanced Drug Delivery Systems with Bicalutamide Based on Mesoporous Silica Particles. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, December 2019. http://dx.doi.org/10.7546/crabs.2019.12.08.

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Palmer, Guy, Varda Shkap, Wendy Brown, and Thea Molad. Control of bovine anaplasmosis: cytokine enhancement of vaccine efficacy. United States Department of Agriculture, March 2007. http://dx.doi.org/10.32747/2007.7695879.bard.

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Anaplasmosis an arthropod-born disease of cattle caused by the rickettsia Anaplasma marginale and is an impediment to efficient production of healthy livestock in both Israel and the United States. Currently the only effective vaccines are derived from the blood of infected cattle. The risk of widespread transmission of both known and newly emergent pathogens has prevented licensure of live blood-based vaccines in the U.S. and is a major concern for their continued use in Israel. Consequently development of a safe, effective vaccine is a high priority. In this collaborative project we focused on two approaches to vaccine development. The first focused o n improving antigen delivery to livestock and specifically examined how DNA vaccines could be improved to enhance priming and expansion of the immune response. This research resulted in development and testing of two novel vaccine delivery systems--one that targeted antigen spread among dendritic cells (the key cell in priming immune responses and a follow-on construct that also specifically targeted antigen to the endosomal-lysosomal compartment the processing organelle within the dendritic cell that directs vaccine antigen to the MHC class ll-CD4* T cell priming pathway). The optimized construct targeting vaccine antigen to the dendritic cell MHC class II pathway was tested for ability to prime A. marginale specific immune responses in outbred cattle. The results demonstrated both statistically significant effects of priming with a single immunization, continued expansion of the primary immune response including development of high affinity lgG antibodies and rapid recall of the memory response following antigen challenge. This portion of the study represented a significant advance in vaccine delivery for livestock. Importantly the impact of these studies is not limited to A. marginale a s the targeting motifs are optimized for cattle and can be adapted to other cattle vaccinations by inserting a relevant pathogen-specific antigen. The second approach (which represented an addition to the project for which approval was requested as part of the first annual report) was a comparative approach between A . marginale and the Israel A . centrale vaccines train. This addition was requested as studies on Major Surface Protein( MSP)- 2 have shown that this antigen is highly antigenically variable and presented solely as a "static vaccine" antigen does not give cross-strain immunity. In contrast A. . centrale is an effective vaccine which Kimron Veterinary institute has used in the field in Israel for over 50 years. Taking advantage of this expertise, a broad comparison of wild type A. marginale and vaccine strain was initiated. These studies revealed three primary findings: i) use of the vaccine is associated with superinfection, but absence of clinical disease upon superinfection with A. marginale; ii) the A. centrale vaccine strain is not only less virulent but transmission in competent in Dermacentor spp. ticks; and iii) some but not all MSPs are conserved in basic orthologous structure but there are significant polymorphisms among the strains. These studies clearly indicated that there are statistically significant differences in biology (virulence and transmission) and provide a clear path for mapping of biology with the genomes. Based on these findings, we initiated complete genome sequencing of the Israel vaccine strain (although not currently funded by BARD) and plant to proceed with a comparative genomics approach using already sequenced wild-type A. marginale. These findings and ongoing collaborative research tie together filed vaccine experience with new genomic data, providing a new approach to vaccine development against a complex pathogen.
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Sampson, James. Current Status and Future Potential for Evaluating the Design and Use of Computer-Based Career Information Delivery Systems in the United States. Florida State University Libraries, December 1993. http://dx.doi.org/10.17125/fsu.1525960033.

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Brice, Jeremy. Investment, power and protein in sub-Saharan Africa. Edited by Tara Garnett. TABLE, October 2022. http://dx.doi.org/10.56661/d8817170.

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The place of protein in sub-Saharan Africa’s food system is changing rapidly, raising complex international development, global health and environmental sustainability issues. Despite substantial growth in the region’s livestock agriculture sector, protein consumption per capita remains low, and high levels of undernourishment persist. Meanwhile sub-Saharan Africa’s population is growing and urbanising rapidly, creating expectations that demand for protein will increase rapidly over the coming decades and triggering calls for further investment in the expansion and intensification of the region’s meat and dairy sector. However, growing disquiet over the environmental impacts of further expansion in livestock numbers, and growing sales of alternative protein products in the Global North, has raised questions about the future place of plant-based, insect and lab-grown proteins in African diets and food systems. This report examines financial investment in protein production in sub-Saharan Africa. It begins from the position that investors play an important role in shaping the development of diets and food systems because they are able to mobilise the financial resources required to develop new protein products, infrastructures and value chains, or to prevent their development by withholding investment. It therefore investigates which actors are financing the production in sub-Saharan Africa of: a) animal proteins such as meat, fish, eggs and dairy products; b) ‘protein crops’ such as beans, pulses and legumes; and c) processed ‘alternative proteins’ derived from plants, insects, microbes or animal cells grown in a tissue culture. Through analysing investment by state, philanthropic and private sector organisations – as well as multilateral financial institutions such as development banks – it aims to establish which protein sources and stages of the value chain are financed by different groups of investors and to explore the values and goals which shape their investment decisions. To this end, the report examines four questions: 1. Who is currently investing in protein production in sub-Saharan Africa? 2. What goals do these investors aim to achieve (or what sort of future do they seek to bring about) through making these investments? 3. Which protein sources and protein production systems do they finance? 4. What theory of change links their investment strategy to these goals? In addressing these questions, this report explores what sorts of protein production and provisioning systems different investor groups might be helping to bring into being in sub-Saharan Africa. It also considers what alternative possibilities might be marginalised due to a lack of investment. It thus seeks to understand whose priorities, preferences and visions for the future of food might be informing the changing place of protein in the region’s diets, economies and food systems.
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Barash, Itamar, and Robert E. Rhoads. Translational Mechanisms that Govern Milk Protein Levels and Composition. United States Department of Agriculture, November 2004. http://dx.doi.org/10.32747/2004.7586474.bard.

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Original objectives: The long term objective of the project is to achieve higher content of protein in the milk of ruminants by modulating the translational machinery in the mammary gland. The first specific aim of the BARD proposal was to characterize responsiveness of various experimental systems to combination of lactogenic hormones and amino acids with particular emphasis on discrimination between the control of total protein synthesis and milk protein synthesis. Based on the results, we planned to proceed by characterizing the stage of protein synthesis in which the stimulation by lactogenic hormones and amino acid occur and finally we proposed to identify which components of the translation machinery are modified. Background to the topic: Milk protein is the most valuable component in milk, both for direct human consumption and for manufacturing cheese and other protein-based products. Attempts to augment protein content by the traditional methods of genetic selection and improved nutritional regimes have failed. The proposal was based on recent results suggesting that the limiting factor for augmenting protein synthesis in the bovine mammary gland is the efficiency of converting amino acids to milk proteins. Major conclusions, solutions, achievements: Insulin and prolactin synergistically stimulate â-casein mRNA translation by cytoplasmatic polyadenylation. The interaction between insulin and prolactin was demonstrated two decades ago as crucial for milk-protein synthesis, but the molecular mechanisms involved were not elucidated. We found in differentiated CID 9 mouse mammary epithelial cells line that insulin and prolactin synergistically increases the rate of milk protein mRNA translation. We focused on â-casein, the major milk protein, and found that the increase in â-casein mRNA translation was reflected in a shift to larger polysomes, indicating an effect on translational initiation. Inhibitors of the PI3K, mTOR, and MAPK pathways blocked insulin-stimulated total protein and â-casein synthesis but not the synergistic stimulation. Conversely, cordycepin, a polyadenylation inhibitor, abolished synergistic stimulation of protein synthesis without affecting insulin-stimulated translation. The poly(A) tract of â-casein mRNA progressively increased over 30 min of treatment with insulin plus prolactin. The 3’-untranslated region of â-casein mRNA was found to contain a cytoplasmic polyadenylation element (CPE), and in reporter constructs, this was sufficient for the translational enhancement and mRNA-specific polyadenylation. Furthermore, insulin and prolactin stimulated phosphorylation of cytoplasmic polyadenylation element binding protein (CPEB) but did not increase cytoplasmic polyadenylation.
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Zamorano, Natalia, and Cristian Herrera. Can community-based intervention packages reduce maternal and neonatal morbidity and mortality? SUPPORT, 2017. http://dx.doi.org/10.30846/170115.

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In the last three decades, rates of neonatal mortality in low-income countries have declined much more slowly than the rates of infant and maternal mortality. A significant proportion of these deaths could potentially be addressed by community-based intervention packages, which are defined as delivering more than one intervention via different sets of strategies that include additional training of outreach workers, building community-support, community mobilization, antenatal and postnatal home visitation, training of traditional birth attendants, antenatal and delivery home visitation, and home-based neonatal care and treatment; usually supplemented by strengthening linkages with local health systems.
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Cook, Stephen, and Loyd Hook. Developmental Pillars of Increased Autonomy for Aircraft Systems. ASTM International, January 2020. http://dx.doi.org/10.1520/tr2-eb.

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Increased automation for aircraft systems holds the promise to increase safety, precision, and availability for manned and unmanned aircraft. Specifically, established aviation segments, such as general aviation and light sport, could utilize increased automation to make significant progress towards solving safety and piloting difficulties that have plagued them for some time. Further, many emerging market segments, such as urban air mobility and small unmanned (e.g., small parcel delivery with drones) have a strong financial incentive to develop increased automation to relieve the pilot workload, and/or replace in-the-loop pilots for most situations. Before these advances can safely be made, automation technology must be shown to be reliable, available, accurate, and correct within acceptable limits based on the level of risk these functions may create. However since inclusion of these types of systems is largely unprecedented at this level of aviation, what constitutes these required traits (and at what level they must be proven to) requires development as well. Progress in this domain will likely be captured and disseminated in the form of best practices and technical standards created with collaboration from regulatory and industry groups. This work intends to inform those standards producers, along with the system designers, with the goal of facilitating growth in aviation systems toward safe, methodical, and robust inclusion of these new technologies. Produced by members of the manned and unmanned small aircraft community, represented by ASTM task group AC 377, this work strives to suggest and describe certain fundamental principles, or “pillars”, of complex aviation systems development, which are applicable to the design and architectural development of increased automation for aviation systems.
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Houzer, Ella, and Ian Scoones. Are Livestock Always Bad for the Planet? Rethinking the Protein Transition and Climate Change Debate. Institute of Development Studies (IDS), September 2021. http://dx.doi.org/10.19088/steps.2021.003.

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Urgent climate challenges have triggered calls for radical, widespread changes in what we eat, pushing for the drastic reduction if not elimination of animal-source foods from our diets. But high-profile debates, based on patchy evidence, are failing to differentiate between varied landscapes, environments and production methods. Relatively low-impact, extensive livestock production, such as pastoralism, is being lumped in with industrial systems in the conversation about the future of food. This report warns that the dominant picture of livestock’s impacts on climate change has been distorted by faulty assumptions that focus on intensive, industrial farming in rich countries. Millions of people worldwide who depend on extensive livestock production, with relatively lower climate impacts, are being ignored by debates on the future of food. The report identifies ten flaws in the way that livestock’s climate impacts have been assessed, and suggests how pastoralists could be better included in future debates about food and the climate.
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Phuong, Vu Tan, Nguyen Van Truong, and Do Trong Hoan. Commune-level institutional arrangements and monitoring framework for integrated tree-based landscape management. World Agroforestry, 2021. http://dx.doi.org/10.5716/wp21024.pdf.

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Governance is a difficult task in the context of achieving landscape multifunctionality owing to the multiplicity of stakeholders, institutions, scale and ecosystem services: the ‘many-multiple’ (Cockburn et al 2018). Governing and managing the physical landscape and the actors in the landscape requires intensive knowledge and good planning systems. Land-use planning is a powerful instrument in landscape governance because it directly guides how actors will intervene in the physical landscape (land use) to gain commonly desired value. It is essential for sustaining rural landscapes and improving the livelihoods of rural communities (Bourgoin and Castella 2011, Bourgoin et al 2012, Rydin 1998), ensuring landscape multifunctionality (Nelson et al 2009, Reyers et al 2012) and enhancing efficiency in carbon sequestration, in particular (Bourgoin et al 2013, Cathcart et al 2007). It is also considered critical to the successful implementation of land-based climate mitigation, such as under Nationally Determined Contributions (NDCs), because the Land Use, Land-Use Change and Forestry (LULUCF) sector is included in the mitigation contributions of nearly 90 percent of countries in Sub-Saharan and Southern Asia countries and in the Latin American and Caribbean regions (FAO 2016). Viet Nam has been implementing its NDC, which includes forestry and land-based mitigation options under the LULUCF sector. The contribution of the sector to committed national emission reduction is significant and cost-effective compared with other sectors. In addition to achieving emission reduction targets, implementation of forestry and land-based mitigation options has the highest benefits for social-economic development and achieving the Sustainable Development Goals (MONRE 2020). Challenges, however, lie in the way national priorities and targets are translated into sub-national delivery plans and the way sub-national actors are brought together in orchestration (Hsu et al 2019) in a context where the legal framework for climate-change mitigation is elaborated at national rather than sub-national levels and coordination between government bodies and among stakeholders is generally ineffective (UNDP 2018). In many developing countries, conventional ‘top–down’, centralized land-use planning approaches have been widely practised, with very little success, a result of a lack of flexibility in adapting local peculiarities (Amler et al 1999, Ducourtieux et al 2005, Kauzeni et al 1993). In forest–agriculture mosaic landscapes, the fundamental question is how land-use planning can best conserve forest and agricultural land, both as sources of economic income and environmental services (O’Farrell and Anderson 2010). This paper provides guidance on monitoring integrated tree-based landscape management at commune level, based on the current legal framework related to natural resource management (land and forest) and the requirements of national green-growth development and assessment of land uses in two communes in Dien Bien and Son La provinces. The concept of integrated tree based landscape management in Viet Nam is still new and should be further developed for wider application across levels.
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