Dissertations / Theses on the topic 'Propionibacterium'
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Perry, Alexandra L. "Characterisation of propionibacterium acnes." Thesis, Aston University, 2004. http://publications.aston.ac.uk/11015/.
Full textGlenn, J. V. "Propionibacterium acnes and medical device infection." Thesis, Queen's University Belfast, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273034.
Full textValanne, M. S. "The pathogenic potential of Propionibacterium acnes." Thesis, Queen's University Belfast, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273147.
Full textFougnot, Sébastien Lozniewski Alain. "Etude de l'activité des antibiotiques sur propionibacterium acnes impliqué dans les infections neuro-méningées." [S.l] : [s.n], 2003. http://www.scd.uhp-nancy.fr/docnum/SCDMED_T_2003_FOUGNOT_SEBASTIEN.pdf.
Full textLan, Annaïg. "Survie et potentialités probiotiques de Propionibacterium freudenreichii." Rennes 1, 2006. http://www.theses.fr/2006REN1S125.
Full textFachin, Luciano. "Contagem de Bifidobacterium animalis Bb 12 e efeito da adição de Propionibacterium freudenreichii PS-1 e do tratamento termico do leite sobre o desenvolvimento de Bifidobacterium animalis Bb 12 em iogurte." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/255805.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: A produção de iogurtes com Bifidobacterium spp. tem crescido muito nas últimas décadas visando a produção de alimentos funcionais. Contudo, para que o produto possa apresentar tais propriedades, tem sido alegado que o número mínimo de células viáveis de Bifidobacterium spp. presentes no momento do consumo deva ser de 106 UFC/g de produto. Entretanto, vários estudos têm mostrado produtos comerciais com contagens menores do que as recomendadas, durante a estocagem do iogurte. Esse fato é reflexo tanto da dificuldade de incorporação destes microrganismos ao iogurte, devido às condições de processo que não são favoráveis ao desenvolvimento da bifidobactéria, bem como pela dificuldade de enumeração destes microrganismos na presença das culturas do iogurte. Este trabalho avaliou o uso dos meios M-MRS, MRS-NNLP, MRS-LP, RCPB pH5 e RCPB pH5 enriquecido com extrato de fígado, visando a contagem seletiva ou diferencial de Bifidobacterium animalis Bb 12 na presença das culturas do iogurte e estudou o efeito do tratamento térmico do leite, visando o aumento do teor de lactulose e, da adição de Propionibacterium freudenreichii PS-1, sobre o desenvolvimento e manutenção do número de células viáveis de B. animalis Bb 12 durante a fermentação e estocagem do iogurte. Dos meios estudados, o meio RCPB pH5, enriquecido com 150mL/L de extrato de fígado, foi o mais indicado para a contagem de B. animalis Bb 12 em iogurte por ter apresentado uma excelente diferenciação deste microrganismo, após a estocagem refrigerada do iogurte. O tratamento térmico do leite de 142°C/15 segundos não afetou o desenvolvimento de B. animalis Bb 12 durante a fermentação do iogurte e também não influenciou a sua resistência à estocagem refrigerada. Entretanto, o tratamento térmico alterou significativamente a textura, diminuindo consideravelmente a dureza, gomosidade e adesividade do iogurte, resultando em um produto com menor separação de soro. A adição de P. freudenreichii PS-1 aumentou em aproximadamente duas vezes o número de células de B. animalis Bb 12 ao final da fermentação e melhorou a resistência da bifidobactéria à estocagem refrigerada. A presença da propionibactéria também alterou significativamente a textura final do iogurte, aumentando consideravelmente a gomosidade e adesividade do produto final, bem como resultou em um iogurte com menor separação de soro durante a estocagem refrigerada
Abstract: Bifidobacterium spp. has been used to produce probiotic yoghurts due to its therapeutic properties. However, it has been claimed that the number of bifidobactéria in yoghurt at the time of consuming must be 106 CFU /g of product to perform their therapeutic functions. Many studies found a low recovery of bifidobactéria from commercial products during shelf life. The low viability of bifidobactéria can be attributed to the process conditions of yoghurt, which is not favorable to the growth of bifidobactéria, and to the difficulty for enumeration of bifidobactéria in the presence of yoghurt bacteria. The objectives of this work were to evaluate the following media: M-MRS, MRS-NNLP, MRS-LP, RCPB pH5 and fortified RCPB pH5 to enumerate B. animalis Bb 12 in yoghurt, and to evaluate the heat treatment of milk (142°C/15 seconds) and the addition of P. freudenreichii PS-1 on the growth of B. animalis Bb 12 during yoghurt fermentation and during shelf life. RCPB pH5 fortified with 150 mL/L of liver extract was the best media due to its excellent differentiation during refrigerated storage of yoghurt. The heat treatment of milk (142°C/15 seconds) did not have effect on the growth of bifidobactéria during fermentation and it also did not improve the bifidus viability during storage. Heat treatment, however, had a strong effect on yoghurt texture, decreasing the sineresis and some parameters of TPA (hardness, gumminess and adhesiveness). The addition of P. freudenreichii PS-1 increased two fold the bifidobactéria growing during yoghurt fermentation and it also increased the viability of bifidobactéria during yoghurt shelf life. The addition of P. freudenreichii PS-1 also decreased the sineresis and increased TPA parameters of hardness, gumminess and adhesiveness. Addition of P. freudenreichii PS-1 to the yoghurt seems to be a good growth promoter for B. animalis Bb 12
Doutorado
Tecnologia de Alimentos
Doutor em Tecnologia de Alimentos
Curiche, Natalia. "Visualization of Propionibacterium acnes in Patients Diagnosed with Acne Vulgaris. - Propionibacterium acnes Detected with Immunofluorescence and Fluorescence in situ Hybridization." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-58623.
Full textVan, der Merwe Iansha (Iansha Rosalia) 1975. "Characterization of thoeniicin 447 produced by Propionibacterium thoenii." Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52729.
Full textENGLISH ABSTRACT: Antimicrobial peptides continue to be one of the most important classes of food additives. The food industry is especially interested in the application of naturally occuring and biologically derived preservatives. Among the metabolites of industrial importance produced by propionibacteria are peptides called bacteriocins. Bacteriocins are ribosomally synthesized peptides with antagonistic activity against closely related microorganisms. Many microorganisms associated with food produce bacteriocins, which have stimulated interest in the use of these peptides as natural food preservatives. Numerous bacteriocins are produced by lactic acid bacteria, but only a few have been reported for propionibacteria. Since propionic acid bacteria have GRAS (generally regarded as safe) status, their metabolic compounds should be safe for human consumption. Propionibacterium thoenii 447, isolated from Emmentaler cheese, produces a bacteriocin-like peptide, named thoeniicin 447, with a narrow spectrum of activity. The peptide displays a bactericidal mode of action against Lactobacillus delbrueckii subsp. bulgaricus and a bacteriostatic action against Propionibacterium acnes. Optimal bacteriocin production was detected during the early stationary growth phase. The peptide is resistant to heat treatments of 60°C and 80°C for 15 and 30 min and to 100°C for 15 min, but loses 80% of its activity after autoclaving (10 min at 121°C). Thoeniicin 447 remains active after incubation in buffers with pH values ranging from 1-10. The peptide is inactivated by pepsin, pronase, a-chymotrypsin, trypsin and Proteinase K. Thoeniicin 447 was partially purified by ammonium sulfate precipitation, followed by SP-Sepharose cation exchange chromatography. The estimated size of thoeniicin 447, according to tricine-SDSPAGE, is approximately 6 kDa. Based on DNA sequencing, the mature peptide is 7130 Da in size and homologous to propionicin Tl produced by P. thoenii strain 419. Thoeniicin 447 is a relatively small, cationic and heat-stable peptide and can therefor be classified as a member of class II bacteriocins. These features are very similar to those of bacteriocins produced by lactic acid bacteria. However, no unique classification system has been proposed for bacteriocins of propionibacteria. As a member of the genus Propionibacterium, P. thoenii 447 is generally regarded as safe. This, together with the narrow spectrum of activity, particularly the action against P. acnes, heat tolerance of thoeniicin 447 and its activity over a wide pH range renders the peptide suitable for possible pharmaceutical applications.
AFRIKAANSE OPSOMMING: Antimikrobiese middels sal deurgaans beskou word as een van die belangrikste klasse van voedsel bymiddels. Die voedselindustrie is veral geïnteresseerd in die toepassing van preserveermiddels van 'n meer natuurlike en biologiese oorsprong. Onder die metaboliese produkte van industriële belang wat deur propionibakterieë geproduseer word is antimikrobiese peptiede (bakteriosiene). Bakteriosiene is ribosomaal-gesintetiseerde peptiede met 'n antagonistiese aktiwiteit teenoor naverwante bakterieë. Verskeie bakteriosiene word deur melksuurbakterieë geproduseer, terwyl slegs enkele vir propionibakterieë beskryf is. Baie van hierdie propionibakterieë word in die algemeen as veilig beskou en het GRAS status. Die metaboliete wat hulle produseer behoort dus veilig vir menslike gebruik te wees. Propionibacterium thoenii 447 is uit Emmentaler kaas geisoleer en produseer 'n bakteriosien-agtige peptied, naamlik thoeniicin 447 met 'n beperkte spektrum van aktiwiteit. Die peptied het 'n bakteriosidiese werking teenoor Lactobacillus delbrueckii subsp. bulgaricus en 'n bakteriostatiese werking teenoor Propionibacterium acnes. Optimum bakteriosien produksie is verkry tydens die vroeë stationêre groeifase. Die peptied is bestand teen hittebehandelings van 60°C en 80°C vir 15 en 30 min, asook 100°C vir 15 min, maar verloor 80% van sy aktiwiteit na outoklavering (lOmin by 121°C). Die peptied blyaktief na inkubasie in buffers van pH 1-10. Die peptied word deur pepsien, pronase, uchymotripsien, tripsien en Proteinase K geïnaktiveer. Thoeniicin 447 is met behulp van ammoniumsulfaat-presipitasie, gevolg deur SPSepharose katioon-uitruilchromatografie gedeeltelik gesuiwer. Skeiding op "n trisien-SDS poliakrielarnied-jel het 'n aktiewe band van ongeveer 6 kDa getoon. Volgens die DNA volgorde bepaling is thoeniicin 447, 7130 Da in grootte en homoloog aan Propionicin Tl, geisoleer vanaf P. thoenii stam 419. Thoeniicin 447 is 'n relatiewe klein, kationiese en hitte-bestande peptied en kan op grond hiervan as 'n lid van die klas II bakteriosiene geklassifiseer word. Hierdie eienskappe is soortgelyk aan die eienskappe van bakteriosiene geproduseer deur melksuurbakterieë. Tot op hede is geen klassifikasiesisteem vir die bakteriosiene van propionibakterieë voorgestel nie. As 'n lid van die genus Propionibacterium, word P. thoenii 447 in die algemeen as veilig beskou. Dit, tesame met die nou spektrum van aktiwiteit, veral teenoor P. acnes, die hittetoleransie van thoeniicin 447, asook die aktiwiteit oor 'n wye pH-grens, maak die peptied geskik vir moontlike farmaseutiese toepassings.
Grassi, Maria Carolina de Barros 1984. "Estudo genético e metabólico da bactéria Propionibacterium acidipropionici." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316784.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O resumo poderá ser visualizado no texto completo da tese digital
Abstract: The abstract is available with the full electronic document
Doutorado
Genetica de Microorganismos
Doutor em Genetica e Biologia Molecular
Vieira, Machado Eduardo Souza. "Culture de propionibacterium freudenreichii et production d'arôme emmental." Compiègne, 1994. http://www.theses.fr/1994COMP734S.
Full textLin, Yu-fei. "Genome-wide analysis of Propionibacterium acnes gene regulation." Thesis, University of Leeds, 2013. http://etheses.whiterose.ac.uk/15231/.
Full textPruitt, Corunda T. "Effects of compatible solutes on cold tolerance of propionibacterium freudenreichii and the significance of propionibacterium cold tolerance in Swiss cheese manufacturing." Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1123184280.
Full textTitle from first page of PDF file. Document formatted into pages; contains xiv, 107 p.; also includes graphics. Includes bibliographical references (p. 90-97). Available online via OhioLINK's ETD Center
Dahlberg, Ida. "Förekomsten av Propionibacterium acnes är låg hos patienter med Rosacea : En studie av sambandet mellan Propionibacterium acnes och Rosacea med immunofluorescens." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-58624.
Full textGuérin, Philippe. "Osteomyelite multifocale a propionibacterium acnes : a propos d'un cas." Clermont-Ferrand 1, 1993. http://www.theses.fr/1993CLF1M031.
Full textMak, Tim Nam [Verfasser]. "Host modulating properties of Propionibacterium acnes / Tim Nam Mak." Berlin : Freie Universität Berlin, 2013. http://d-nb.info/1033306703/34.
Full textJoubert, Hannarine. "Optimisation of propionibacterial ECP production and the influence of propionibacteria on the UASB granulation process." Thesis, Stellenbosch : Stellenbosch University, 2000. http://hdl.handle.net/10019.1/51866.
Full textENGLISH ABSTRACT: The "classical" propionibacteria are used in a variety of natural dairy fermentations where they produce natural preservatives (propionic and acetic acids and bacteriocins) and large amounts of vitamin B12. The extracellular polysaccharide (ECP) producing ability of these bacteria also make them of special interest to the food and waste water management industries as the ECP has been illustrated to playa role in the initial granule formation in upflow anaerobic bioreactor systems. There is little known on the ECP production by propionibacteria and in this study different environmental conditions that influence ECP production were studied. Nineteen different Propionibacterium strains were examined in terms of ECP production and Propionibacterium strain 278 was identified as the best ECP producer. Further studies were only done on this strain because of its high ECP production and because it was originally isolated from an anaerobic digester. The influence of temperature, pH and sucrose concentration was determined through the measurement of ECP production and medium viscosity. It was found that more ECP was produced at temperatures lower than the optimum for growth with the optimum being between 22° and 25°C. Lower initial pH conditions of the growth medium (below pH 7.0) were found to inhibit ECP production and the influence when the initial pH values were between 7.0 and 8.5, was not significant. A higher carbon: nitrogen ratio, when 8% sucrose was added, was also found to enhance the ECP production. The upflow anaerobic sludge bed (UASB) bioreactor process depends on the upward movement of soluble matter through a blanket of active methanogenic granular sludge. The long start-up times as a result of the slow granulation process, as well as the need for a speedy replacement of granules once they have been washed out of the system, are limitations that restrict the general application of this excellent waste water treatment technology. Full exploitation of this biomass immobilisation technique can thus not be realised until the granule formation conditions are defined and optimised. The precise nature of the mechanisms involved in the formation of granules and the reason for their stability, is still not fully understood. It was hypothised by Britz et al. in 1999 that, through the implementation of environmental 'stress' conditions, a shift in the population dynamics of the anaerobic community can be obtained. This results in a concurrent increase in ECP formation that appears to enhance aggregate formation. In the second study it was found that, when 'stress' conditions were applied to already formed granules, the Gram-positive lactate-utilising acidogenic population gained an advantage and more propionic acid producing bacteria were present. The propionic and acetic acid concentrations were also found to increase, and concurrently, a decrease in the growth medium pH occurred. This confirms part of the granulation hypothesis that, when granules are 'stressed', the acidogenic population dynamics change and the lactate-utilising population responds to the gradual decrease in pH and the more acid-tolerant propionic acid producing bacteria gain a competitive advantage resulting in the increase in the propionic acid concentration. When propionibacteria were added to raw sludge during the granule production process, the granules were found to be more active than when nopropionibacteria had been added. This was probably due to the ECP formation by the propionibacteria that enhances the aggregation of the granules. Enhanced granulation was thus found in the batch systems with the fatty acids formed in correlation with the model for granulation. A good correlation was evident between the hypothesis and the experimental data and the hypothesis was partially verified in this study.
AFRIKAANSE OPSOMMING: Die "klassieke" propionibakterieë word in 'n verskeidenheid van natuurlike suiwel fermantasies gebruik waarin hulle verantwoordelik in vir die produksie van natuurlike voedsel preserveermiddels (propioonsuur, asynsuur en bakteriosiene) en groot hoeveelhede vitamiene B12. Die Ekstra Sellulêre Pollisakkaried (ESP) produserende eienskap van hierdie groep bakterieë maak hulle ook van belang in die voedsel en afvoerwater beheer industrieë, aangesien gevind is dat ESP 'n rol speel in die aanvanklike granule formasie in anaerobiese bioreaktor sisteme. Daar is nog baie min bekend oor die ESP produksie van propionibakterieë en in hierdie studie is verskeie omgewings faktore wat die ESP produksie beïnvloed, bestudeer. Negentien verskillende Propionibakterium stamme was bestudeer in terme van ESP produksie en Propionibakterium stam 278 was geïdentifiseer as die stam wat die meeste ESP produseer. Verdere studies was op hierdie stam gedoen na aanleiding van sy hoë ESP produksie en omdat dit oorspronklik uit 'n anaerobiese verteerder geisoleer is. Die invloed van termperatuur, pH en sukrose konsentrasie was bepaal deur die meting van die ESP produksie en die medium viskositeit. Dit was gevind dat meer ESP geproduseer was by temperature laer as die optimum vir groei, met die optimum temperatuur tussen 22° en 25°C. Dit is ook gevind dat laer aanvangs groei-medium pH (laer as pH 7.0), ESP produksie inhibeer. Die invloed van die aanvangs groei-medium pH tussen 7.0 en 8.5 was egter nie betekenisvol nie. Dit is ook gevind dat 'n hoër koolstof tot stikstof verhouding, verkry deur die byvoeging van 8% sukrose, die ESP produksie verhoog. Die "upflow anaerobic sludge blanket" (UASB) proses vind plaas as gevolg van die opwaarste beweging van opgeloste organiese materiaal deur 'n granule bed van aktiewe metanogeniese granulêre slyk. Die lang 'start-up' tye as gevolg van die stadige granulasie proses, en die nodigheid om 'n vinnige verplasing van granules te hê nadat dit uit die sisteem gewas is, is beperkings wat die algemene toepassing van hierdie fantastiese afvoerwater tegnologie, strem. Volle implementering van hierdie biomassa immobilisereings tegniek kan dus nie plaasvind voordat die granule formasie gedefinieer en geoptimiseer is nie. Die presiese eienskappe van die meganismes betrokke en die formasie van die granules en die rede vir hul stabiliteit word egter nog nie ten volle verstaan nie. Volgens 'n hipotese deur Britz et al. (1999), vind 'n verskuiwing in die populasie dinamika van die anaerobiese gemeenskap plaas tydens die implementasie van omgewings 'stress' toestande. Die resultaat is 'n verhoging in ESP produksie en 'n gevolglike verbetering in die granulasie proses. In die tweede studie was dit gevind dat, wanneer 'stress' toestande op die reeds gevormde granulasie toegepas word, die Gram-positiewe laktaat-benuttende asetogeniese populasie voordeel geniet en meer propioonsuur produserende bakterieë was teenwoordig. Die propioonsuur en asynsuur konsentrasies het ook verhoog en met 'n gevolglike daling in die groei-medium se pH. Dit bevestig 'n gedeelte van die hipotese dat, wanneer die granules onder 'stress' geplaas word, die asetogeniese populasie dinamika verander en die laktaat-benuttende populasie reageer tot die gedeeltelike afname in pH. Die meer suur-tolerante propioonsuur produserende bakterieë verkry 'n kompeterende voordeel en gevolglik is daar 'n verhoging in propioonsuur konsentrasie. Propionibakterieë was gevoeg by die onbehandelde slyk gedurende die granule produksie proses, en daar is gevind dat meer aktiewe granules gevorm word as andersins. Dit is moontlik as gevolg van die die ESP produksie van propionibakterieë wat die granulasie versnel het. Verbeterde granulasie was dus verkry in die sisteme waar propionibakterieë bygevoeg is. Vetsuur analises het gedui dat die gevormde vetsure ook in korrelasie was met die model van granulasie. Goeie korrelasie was dus verkry tussen die hipotese en die eksperimentele data en die hipotese is gedeeltelik bewys in hierdie studie.
Whale, Gary Anthony. "Purification and characterisation of the lipid macroamphiphiles of propionibacterium acnes." Thesis, Robert Gordon University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270026.
Full textBassalo, Marcelo Colika 1989. "Estudo do metabolismo aeróbico da bactéria anaeróbica facultativa Propionibacterium acidipropionici." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316761.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: A sociedade atual é fundamentalmente dependente do petróleo, recurso natural inserido na grande maioria dos setores da economia. Entretanto, fatores como a limitada disponibilidade deste recurso, sua instabilidade no mercado devido a problemas de natureza geopolítica e a emissão de dióxido de carbono ocasionada pela utilização deste combustível, acentuaram as iniciativas para substituir o petróleo por fontes alternativas e renováveis de matéria prima. A bactéria Propionibacterium acidipropionici surge como uma excelente candidata para a substituição de compostos petroquímicos, através da produção do ácido propiônico. No entanto, antes de transformar esta bactéria em uma plataforma industrial, é necessário aprofundar a compreensão do metabolismo deste microrganismo e desenvolver ferramentas de manipulação genética. No que diz respeito à compreensão do metabolismo, poucos estudos avaliaram o perfil aeróbico desta bactéria, considerada anaeróbica estrita até recentemente. No presente trabalho, foi identificada nesta bactéria a presença de todos os componentes de uma cadeia transportadora de elétrons. No entanto, a citocromo c oxidase identificada apresenta-se mutada e os testes realizados confirmaram a não funcionalidade deste complexo. A existência de uma oxidase alternativa, a citocromo bd oxidase, caracterizada pela alta afinidade ao oxigênio, surge então como uma hipótese promissora acerca da microaerofilia desta bactéria. O trabalho também avaliou o perfil fermentativo dessa bactéria em condições aeróbicas com diferentes fontes de carbono, o que ressaltou a enorme flexibilidade metabólica apresentada por P. acidipropionici, capaz de redirecionar o fluxo de carbono para diferentes produtos finais a depender da necessidade de manutenção do balanço redox. Este estudo também revelou uma propriedade bastante peculiar e industrialmente relevante do xarope de cana-de-açúcar. A fermentação aeróbica com este substrato, ao contrário de todas as outras fontes de carbono, apresentou um crescimento superior ao das condições anaeróbicas e, adicionalmente, exibiu um perfil fermentativo próximo ao observado em ausência de oxigênio. A identificação do composto presente no xarope de cana-de-açúcar, responsável por simular o metabolismo anaeróbico, poderia viabilizar a produção do ácido propiônico em dornas de fermentação aeróbicas, o que traria enormes benefícios para a produção economicamente viável do ácido propiônico e na implementação de P. acidipropionici como uma plataforma industrial
Abstract: The dependence of contemporary society on petroleum is axiomatic, and this natural resource could be found intrinsically embedded in the vast majority of economic sectors. Nonetheless, the limited availability of this natural resource, the instability in the stock market due to geopolitical problems, and also the carbon dioxide emissions associated with the use of fossil fuels have highlighted the need to search for renewable energy sources. The bacteria Propionibacterium acidipropionici arises as an excellent strategy for the substitution of petrochemical compounds, through the production of propionic acid. Before we could implement this bacterium as an industrial platform, however, it becomes necessary to enhance the knowledge regarding the metabolism of P. acidipropionici, and thus create a backbone for the development of genetic manipulation tools. Regarding the metabolism of this bacterium, there aren't comprehensive studies about its aerobic metabolism, thus being considered strict anaerobes until recently. In the present work, it was identified that P. acidipropionici has all required components for a functional electron transport chain. However, the cytochrome c oxidase of this bacterium has a frameshift mutation, and the functional studies proved that this complex is not operative. The presence of an alternative oxidase of high oxygen affinity, called cytochrome bd oxidase, is then suggested as a hypothesis to explain the microaerofilic habit of this bacterium. This work has also shed light into the fermentative profile showed by this bacterium under aerobic cultivation with different carbon sources, bringing attention to the highly flexible metabolism of P. acidipropionici. This bacterium has shown to be capable of completely changing its carbon flux to different end products, as a strategy to maintain the redox balance. In addition, this work has also unveiled an interesting and industrially-relevant property of the sugar cane syrup. It was demonstrated that the aerobic cultivation of P. acidipropionici with sugar cane syrup increased the culture growth, as well as it changed the fermentation end products in a way more similar to the anaerobic cultivation. It was hypothesized that this unusual property found in the sugar cane syrup was due to the presence of a mineral compound that could be used as a final electron acceptor by P. acidipropionici. The identification of this specific compound would allow the aerobic production of propionic acid in industrial conditions, and thus could be a major breakthrough to turn its industrial production into an economically viable process
Mestrado
Genetica de Microorganismos
Mestre em Genética e Biologia Molecular
Calabrese, Ana Paula Mazine. "Estudos da inativação de Propionibacterium acnes por fotodinamização de hipericina." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/82/82131/tde-04012013-161203/.
Full textOne of the prominent challenges in medical dermatology has been the treatment of acne. The acne affects 80 to 90% of teenagers. The search for alternative treatment has become important due to bacterial resistance of P. acnes to usually applied antibiotics against this etiologic agent and the side effects produced by these drugs. In order to overcome these limitations to inactivated the P. acnes, the photodynamic based protocols has been studied. Already, well established named photodynamic therapy has been used against many cancers and the use of light has shown promise in the esthetic area, the photodynamic protocols has been used to treat acne based on endogenous synthesis of hematoporphyrin and related compounds induced by -aminolevulinic acid, a long term treatment and using the blue light. The objective of this study was to evaluate the effectiveness of photodynamic inactivation of the P. acnes using hypericin, irradiated by a yellow LED (590 nm). The microorganism inactivation was achieved even at low concentration of hypericin (less than 1 \'mü\'g/mL), and was characterized by the short time bioaccumulation stationary state (around 2.5 minutes). In order to guarantee the reproducibility typically the incubation time was 10 minutes. The photodynamic efficiency was evaluated to be 4.55 ± 0.08 J \'CM POT.-2\' able to reduce 63% of the viable cells using 10 \'mü\'g/mL of hypericin. These results allow concluding that the hypericin is an effective FS to inactivate P. acnes.
Davis, Nigel. "The structural genes for methylmalonyl-CoA metabolism in Propionibacterium shermanii." Thesis, University of Cambridge, 1986. https://www.repository.cam.ac.uk/handle/1810/270400.
Full textLEMEE, RIWANON. "Systeme autolytique de propionibacterium freudenreichii cnrz 725 bacterie propionique laitiere." Rennes 1, 1994. http://www.theses.fr/1994REN10198.
Full textSuwannakham, Supaporn. "Metabolic engineering for enhanced propionic acid fermentation by Propionibacterium acidipropionici." Connect to this title online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1111728310.
Full textTitle from first page of PDF file. Document formatted into pages; contains xix, 258 p.; also includes graphics (some col.) Includes bibliographical references (p. 199-212). Available online via OhioLINK's ETD Center
Duarte, Juliana Canto 1984. "Produção de ácidos orgânicos C-3 e C-4 através da fermentação de diferentes substratos por Propionibacterium acidipropionici." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/266058.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Química
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Resumo: Foram realizadas fermentações em batelada do sorbitol, sacarose, glicerol e glicose por Propionibacterium acidipropionici livre em biorreatores. As fermentações do sorbitol forneceram a maior concentração final de ácido propiônico (39,5±5,2 g L-1) e as fermentações da glicose forneceram a menor concentração final de ácido propiônico (23,9±2,1 g L-1). Apenas as fermentações do glicerol produziram n-propanol e sua concentração final foi de 1,7±0,1 g L-1, além disso, o ácido acético não foi produzido nas fermentações do glicerol. As fermentações da sacarose forneceram a maior concentração final de ácido acético (10,9±0,0 g L-1). O maior valor de produtividade do ácido propiônico foi obtido nas fermentações do sorbitol (0,60 g L-1 h-1). Fermentações do sorbitol e da sacarose em batelada utilizando células de P. acidipropionici livres e imobilizadas em montmorilonita K10 foram realizadas em três ciclos sequenciais com reciclo celular. As concentrações finais de ácido propiônico para as fermentações do sorbitol com células livres foram 39,5±5,2; 35,8±1,4 e 34,4±1,9 g L-1 e para células imobilizadas foram 33,1±0,7; 37,2±0,6 e 36,6±0,4 g L-1, para o primeiro, segundo e terceiro ciclos sequenciais, respectivamente. O maior valor de produtividade e de rendimento do ácido propiônico nas fermentações do sorbitol foi obtido para o primeiro ciclo com células livres: 0,60 g L-1 h-1 e 0,613 g g-1, respectivamente. As concentrações finais de ácido propiônico para as fermentações da sacarose com células livres foram 33,4±0,3; 31,3±0,9 e 31,1±0,1 g L-1 e para células imobilizadas foram 26,9±0,6; 29,1±0,3 e 29,5±0,8 g L-1 para o primeiro, segundo e terceiro ciclos sequenciais, respectivamente. A produtividade e o rendimento do ácido propiônico foram maiores no primeiro ciclo para células livres: 0,48 g L-1 h-1 e 0,409 g g-1, respectivamente. Foram realizadas co-fermentações de glicose:glicerol em duas razões mássicas (1:1 e 2:1) em batelada utilizando P. acidipropionici livre e imobilizada em montmorilonita K10. Apenas nas co-fermentações foi observada a produção do ácido lático e trealose além dos ácidos acético, succínico e propiônico. A maior concentração final de ácido propiônico foi obtida para as co-fermentações glicose:glicerol de razão mássica 2:1 com células de P. acidipropionici livres (31,4±0,3 g L-1) e a menor concentração final foi obtida nas co-fermentações de razão mássica 1:1 para células imobilizadas (23,9±0,8 g L-1). O ácido acético foi produzido apenas nas co-fermentações de razão mássica 2:1 para células livres (0,3±0,4 g L-1) e imobilizadas (1,1±0,2 g L-1). A maior concentração final de ácido lático foi obtida nas co-fermentações de razão mássica 1:1 para células livres (10,5±0,3 g L-1) e a menor concentração final foi obtida nas co-fermentações de razão mássica 2:1 para células livres (0,9±0,6 g L-1). A maior concentração final de trealose foi obtida nas co-fermentações de razão mássica 2:1 para células imobilizadas (12,1±0,1 g L-1) e a menor concentração final foi obtidas nas co-fermentações de razão mássica 2:1 para células livres (6,7±1,0 g L-1). O maior valor de produtividade e de rendimento do ácido propiônico foram obtidos nas co-fermentações de razão mássica 2:1 para células livres: 0,45 g L-1 h-1 e 0,412 g g-1, respectivamente
Abstract: Batch fermentations of sorbitol, sucrose, glycerol and glucose by free cells of Propionibacterium acidipropionici were conducted in bioreactors. Sorbitol fermentations yield the major final propionic acid concentration (39.5±5.2 g L-1) while glucose fermentations yield the minor result (23.9±2.1 g L-1). Only glycerol fermentations yield n-propanol (1.7±0.1 g L-1), furthermore, acetic acid was not generated in glycerol fermentations. In the other hand, sucrose fermentations yield great acetic acid final concentration (10.9±0.0 g L-1). The major propionic acid productivity was got in sorbitol fermentations (0.60 g L-1 h-1). Sorbitol and sucrose batch fermentations by free and immobilized cells of P. acidipropionici in montmorillonite K10 were carried out in three sequential cycles with cell reuse. The final propionic acid concentrations to sorbitol with free cells were 39.5±5.2; 35.8±1.4 e 34.4±1.9 g L-1 and with immobilized cells were 33.1±0.7; 37.2±0.6 e 36.6±0.4 g L-1, to the first, second and third sequential cycles, respectively. The major propionic acid productivity was got in the first cycle with free cells to sorbitol fermentations (0.60 g L-1 h-1). The major propionic acid yield was 0.613 g g-1 in the first cycle to free cells. The final propionic acid concentrations to sucrose fermentations with free cells were 33.4±0.3; 31.3±0.9 e 31.1±0.1 g L-1 and to immobilized cells were 26.9±0.6; 29.1±0.3 e 29.5±0.8 g L-1 to the first, second and third sequential cycles, respectively. The propionic acid productivity was major in the first cycle to free cells (0.48 g L-1 h-1). The major propionic acid yield was 0.409 g g-1 in the first cycle to free cells. Batch co-fermentations of glucose and glycerol were also conducted in two different mass ratios (1:1 and 2:1 glucose:glycerol) by P. acidipropionici free and immobilized in montmorillonite K10. The major propionic acid final concentration was got in glucose:glycerol 2:1 mass ratio co-fermentations by free cells (31.4±0.3 g L-1) while the minor final concentration was got with co-fermentation 1:1 mass ratio to immobilized cells (23.9±0.8 g L-1). Acetic acid was got only in co-fermentations 2:1 mass ratio to free (0.3±0.4 g L-1) and immobilized (1.1±0.2 g L-1) cells. The major propionic acid productivity was got in co-fermentations 2:1 mass ratio to free cells (0.45 g L-1 h-1). The major propionic acid yield was got in co-fermentations 2:1 mass ratio to free cells (0.412 g g-1). Only co-fermentations yield lactic acid and trehalose. The major lactic acid final concentration was got in co-fermentations 1:1 mass ratio to free cells (10.5±0.3 g L-1) and the minor final concentration was got in co-fermentations 2:1 mass ratio to free cells (0.9±0.6 g L-1). The major trehalose final concentration was got in co-fermentations 2:1 mass ratio to immobilized cells (12.1±0.1 g L-1) and the minor final concentration was got in co-fermentations 2:1 mass ratio to free cells (6.7±1.0 g L-1)
Doutorado
Engenharia de Processos
Doutora em Engenharia Quimica
Liao, Yu-Hua. "Polymerase chain reaction based cloning of acetate kinase in Propionibacterium acidipropionici /." Connect to this title online, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1072778140.
Full textMeurice, Guillaume. "Reconstruction in silico de voies métaboliques : application aux voies glycolitiques de Propionibacterium freudenreichii subsp. shermanii." Rennes, Agrocampus Ouest, 2004. http://www.theses.fr/2004NSARI041.
Full textCousin, Fabien. "Le premier lait fermenté exclusivement par une bactérie propionique laitière : mise au point et potentiel probiotique." Rennes, Agrocampus Ouest, 2012. http://www.theses.fr/2012NSARI066.
Full textDairy propionibacteria are mainly used as ripening starters in Swiss-type cheeses such as Emmental cheese. Moreover, probiotic properties have been described for these bacteria. Some of these effects require high populations of live and metabolically active dairy propionibacteria in the colon. To date, no fermented dairy product exists to study specifically the beneficial effects of these bacteria in the colon. As dairy matrix protects probiotics from stresses undergone in the digestive tract, a milk exclusively fermented by dairy propionibacteria would allow to explore its probiotic potential. The aim of this thesis was to develop a milk exclusively fermented by a dairy propionibacterium and to test it as a probiotic vehicle to study the probiotic properties of this bacterium. A new milk exclusively fermented by dairy propionibacteria, in which the bacteria population reached 109 bacteria per milliliter, has been designed. It protected propionibacteria from digestive stresses in vitro and in vivo. It induced apoptosis (programmed cell death) in human colon and gastric cancer cells in vitro. This fermented milk also displayed an immunomodulatory potential in vitro and in vivo, in favor of an anti-inflammatory pattern. A powerful tool to study the potential benefits of dairy propionibacteria is now available for the scientific community. It will allow to explore the health benefits of these bacteria, suggested in vitro, in preclinical and clinical studies
Silva, José Bruno Nunes Ferreira da. "Avaliação da atividade imunomoduladora de Propionibacterium acnes em animais submetidos à sepse letal e perfuração do ceco (CLP)." Universidade Federal de Pernambuco, 2012. https://repositorio.ufpe.br/handle/123456789/12238.
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CAPES
Sepse é uma resposta inflamatória sistêmica que apresenta falha na resposta imune do hospedeiro associado à infecção. Propionibacterium acnes é uma bactéria conhecida por sua atividade imunomoduladora in vitro e in vivo. Neste estudo, foi avaliado o efeito da P. acnesinativada por fenol, na formulação comercial Imunoparvum®, sobre a infecção polimicrobiana induzida por ligadura e perfuração cecal (CLP). Camundongos albinos suíços machos (Mus musculus) foram divididos em 5 grupos (n=8-16/grupo). A administração de salina 0,9% (grupo controle S-CLP) ou Imunoparvum®(grupo tratado) foi realizada 1, 3, 5 e 7 dias antes da CLP. A CLP foi realizada no oitavo dia. A taxa de sobrevida foi analisada com oito animais de cada grupo.Para determinação do número de células peritoneais, citocinas, contagem bacteriana e MPO, os animais foram sacrificados 6 h após a indução de sepse e tiveram a cavidade peritoneal lavada com PBS + EDTA. O grupo tratado comImunoparvum®mostrou aumento da taxa de sobrevivência de 50% em 96 horas. O tratamento com Imunoparvum®casusou um aumento na migração celular para o peritôneo, induziu uma significante redução no lavado peritoneal da citocinas pró-inflamatórias TNF-α, MCP-1 e a citocina antiinflamatória IL-10 (TNF-α de 112,89±7,31pg/mL para 61,04±18,93 pg/mL, MCP-1 de 1321,98 ± 3,84 pg/mL para 778,89±1,24 pg/mL e IL-10 de 1837,41 ± 173,87 pg/mL para 718,80 ± 47,52 pg/mL). Por outro lado, houve um aumento nas concentrações de IL-6 (de 340,33 ± 11,48 pg/mL para 416,89 ± 8,14 pg/mL) no grupo tratado em relação ao controle. Não houve diferença nos níveis de mieloperoxidase (MPO) no pulmão dos animais do grupos Imunoparvum® e controle. O tratamento com Imunoparvum® reduziu o número de bactérias viáveis na cavidade peritoneal. De acordo com os resultados, Imunoparvum®promoveu o aumento da taxa de sobrevida de animais com sepse, em parte atribuída às suas propriedades imunomoduladoras, importantes no combate de microorganismos patogênicos, bem como ao melhor comtrole da infecção através da redução da contagem bacteriana.
Figueira, Aburjaile Flavia. "Mécanismes moléculaires de la survie à long terme chez Propionibacterium freudenreichii." Thesis, Rennes, Agrocampus Ouest, 2015. http://www.theses.fr/2015NSARB273/document.
Full textPropionibacterium freudenreichii is a dairy bacterium belonging to the Actinobacteria group, which is known to survive for long periods in harsh environmental conditions. In order to investigate the long-term survival phenomenon in P. freudenreichii, 8 strains were phenotypically characterized for a period of 11 days in nutrient shortage condition. Bacterial survival rate was assessed by optical density, CFU counting and live-dead cellular viability. In addition, the absence of cell lysis was evaluated by quantitative PCR. P. freudenreichii growth phases were classified as exponential, stationary, late stationary and long-term survival. Moreover, it was observed that bacterial viability was maintained during long-term survival.Phenotypical characterization indicated that P. freudenreichii CIRM-BIA138 was more resistant to nutrient shortage being able to enter into a viable but nonculturable dormant state. In addition, functional studies of this strain were conducted by RNA-Seq on cultures sampled in exponential and stationary growth phases. Concomitantly, several biochemical analyses were carried out on the culture supernatant. An integrative approach of metabolomic and transcriptomic data allowed us to infer strategies associated with the survival of this bacterium, such as preparation for the dormant state, slow down of metabolic activity and utilization of alternative sources of energy, which altogether might allow P. freudenreichii CIRM-BIA 138 to adapt and persist through nutrient shortage for long periods
Ryan-Kewley, Angela E. "Microbial ecology of Propionibacterium acnes in patients undergoing treatment with isotretinoin." Thesis, University of Lincoln, 2011. http://eprints.lincoln.ac.uk/6073/.
Full textDe, Annunzio Sarah Raquel. "Atividade antimicrobiana sinérgica da terapia fotodinâmica e tetraciclina contra Propionibacterium acnes /." Araraquara, 2017. http://hdl.handle.net/11449/152524.
Full textBanca: Ilana Lopes Baratella da Cunha Camargo
Banca: Juliana Cabrini Carmello
Resumo: A Acne vulgar é uma das mais comuns doenças dermatológicas atingindo adolescentes e adultos. A principal causadora da acne é a infecção pela bactéria Propionibacterium acnes (P. acnes). Atualmente, as opções de tratamento para acne vulgar são numerosas, porém muitas vezes com extensos efeitos colaterais. Fatores como resistência aos antibióticos, e a ação inicial lenta das terapias tópicas têm levado pesquisadores a buscar tratamentos alternativos. Desta forma, o presente estudo objetivou avaliar a eficácia da inativação fotodinâmica mediada por curcumina dissolvida em caldo (TrypcticSoyBroth) TSB e solução de sacarose 0,5% e a combinação dessa terapia com a tetraciclina, contra P. acnes em fase planctônica e biofilme. A viabilidade celular foi avaliada através da quantificação das colônias formadas por mililitro de amostra (UFC/mL). A concentração bactericida mínima (CBM) da tetraciclina sobre P. acnes foi determinada através do método de microdiluição em caldo. Foi realizada a avaliação da biomassa total e inibição da formação do biofilme através do método de coloração por cristal violeta 0,5%. A análise estatística dos dados foi feita através do teste de Análise de Variância (oneway ANOVA) com pós-teste de Tukey. Os resultados do presente estudo mostraram que a terapia fotodinâmica antimicrobiana (TFDa) mediada por curcumina foi eficiente na redução microbiana quando realizada a quantificação das colônias formadas, comparada ao grupo controle negativo em forma planctônica ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Acne vulgar is one of the most common dermatological and adult diseases. The main cause of acne is an infection by the bacterium Propionibacterium acnes (P. acnes).Currently there are many treatment options for acne vulgaris, although often with extensive side effects. Factors such antibiotics resistance and slow initial action of topical therapies that led researchers to seek alternative treatments. Thus, the present study aimed to evaluate the efficacy of curcumin-mediated photodynamic inactivation in broth (Tryptic Soy Broth) TSB and 0.5% sucrose solution and the combination of this therapy with tetracycline against planktonic phase P. acnes and biofilm. Cell viability was evaluated by quantification of the colonies formed per milliliter of sample (CFU / mL). The minimum bactericidal concentration (MBC) of tetracycline over P. acnes was determined by the broth microdilution method. The evaluation of the total biomass and inhibition of the formation of the biofilm was performed through the method of staining by violet crystal 0.5%. The statistical analysis of the data was done through the variance analysis (ANOVA one way) test with Tukey's post test. The results of this study showed that curcumin-mediated photodynamic antimicrobial therapy (TFDa) was efficient in microbial reduction when quantification of the colonies was performed, compared to the negative control group in planktonic form as well as in biofilm both diluted in TSB broth and in solution sucrose 0.5% being able to reduce all bacterial load. A reduction of more than 28% of the total biofilm biomass and an inhibition of more than 70% was achieved after treatment. It was possible to conclude that curcuminmediated TFDa dissolved in TSB broth and 0.5% sucrose solution was efficient for the total reduction of the planktonic and biofilm microbial load. Curcumin... (Complete abstract click electronic access below)
Mestre
Liao, Yu-Hua. "Polymerase chain reaction based cloning of acetate kinase in propionibacterium acidipropionici." The Ohio State University, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=osu1072778140.
Full textMurano, Elsa Alina. "Role of polymorphonuclear leukocytes in the tumoricidal activity of Propionibacterium acnes." Thesis, Virginia Polytechnic Institute and State University, 1987. http://hdl.handle.net/10919/50069.
Full textMaster of Science
incomplete_metadata
Jarrousse, Véronique. "Rôle de Propionibacterium acnes dans la différenciation et l'immunité innée kératinocytaire." Nantes, 2006. http://archive.bu.univ-nantes.fr/pollux/show.action?id=7bef751e-1ac8-4cb6-b817-0979934f8e0f.
Full textThe aim of this work was to determinate the effects of Propionibacterium acnes in keratinocytes differentiation and innate immunity. Our results suggest that P. Acnes play a part in abnormal keratinocytes differentiation indeed P. Acnes extract modulate integrins and filaggrin expression. We also show that FM P. Acnes extract induce TLR2 expression. Zinc could partially inhibit this expression. In conclusion, P. Acnes seems to play an important part in acne physiopathology from microcomedon to the inflammation. Concurrently, we have studied innate immunity modulation in cutaneous T cell lymphoma (CTCL). The origin of this pathology remains unknown but the implication of viruses is highly suspected. We show that TLRs 2, 4 and 9 expressions by keratinocytes was increase in CTCL epidermis compare to normal skin. Furthermore TLRs 2, 4 and 9 pattern expression in MF skin was different to that observed in SS skin. In conclusion, the activation of TLRs 2, 4 and 9 by an infectious antigen could play a role in the chronic activation of T lymphocytes in the skin of CTCL patients
Limpisathian, Patcharee. "Characterization of the interaction between Lactobacillus helveticus and Propionibacterium in Swiss Cheese." Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1123789201.
Full textTitle from first page of PDF file. Document formatted into pages; contains xvii, 143 p.; also includes graphics. Includes bibliographical references (p. 106-111). Available online via OhioLINK's ETD Center
Calderón, Luige Armando Llerena 1986. "Estudo de otimização das condições da fermentação para produção de ácido propiônico por Propionibacterium acidipropionici utilizando xarope de cana-de-açúcar." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314278.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O ácido propiônico possui grande importância para a economia devido às suas diversas aplicações nas indústrias químicas e alimentícias. Atualmente, ele é produzido a partir de derivados do petróleo, mas as atuais preocupações ambientais relacionadas às emissões de carbono provenientes de fontes fósseis tornam a sua produção por rotas biológicas e fontes renováveis um processo cada vez mais atraente. O ácido propiônico também pode ser produzido a partir do metabolismo heterofermentativo da Propionibacterium acidipropionici. Embora existam diversos estudos que visem à otimização do processo de produção de ácido propiônico por rotas biológicas, ainda não existe nenhum processo eficiente que possa ser escalonado a um nível industrial. Consequentemente, o foco deste trabalho está no uso de ferramentas de planejamento experimental para a produção de ácido propiônico a partir de xarope de cana-de-açúcar (caldo de cana-de-açúcar concentrado), levando-se em conta três respostas de extrema importância para este bioprocesso: rendimento, produtividade volumétrica e a resposta de menor formação de ácido succínico (RMS). Na primeira parte do trabalho foi realizada a adequação de um método cromatográfico para a quantificação dos açúcares presentes no xarope de cana-de-açúcar, a identificação da linhagem P. acidipropionici ATTC 4875 como a mais adequada para a produção de ácido propiônico e o estudo do papel do extrato de levedura como fonte de nitrogênio para o meio de cultura. Utilizando-se o delineamento experimental Plackett & Burman, foram estudados 8 fatores com o objetivo de avaliar a sua importância no processo. Dos 8 fatores, três (temperatura, concentração de extrato de levedura e concentração de xarope de cana-de-açúcar) foram selecionados por apresentarem influência significativa no processo de produção de ácido propiônico. Em uma segunda etapa, utilizando um delineamento composto central rotacional (DCCR), foram determinados os valores ótimos desses fatores como sendo 35 ºC de temperatura, 22 g/L de extrato de levedura e 54 g/L de concentração de xarope de cana-de-açúcar. Com estas condições, obteve-se um rendimento de 47% (m/m), uma produtividade volumétrica de 0,89 g/L. h e uma RMS de 94%. Estes resultados significaram um incremento de 5%, 196% e 8% no rendimento, na produtividade volumétrica e na RMS, respectivamente, quando comparados à condição de cultivo inicial
Abstract: Propionic acid is of great importance for economy because of its variety of applications in the chemical and food industries. It is currently produced from petroleum, but due to environmental concerns related to carbon emissions from fossil fuels the production based on renewable biological routes is becoming more attractive. Propionic acid can be produced through the heterofermentative metabolism of Propionibacterium acidipropionici. Despite the existence of several studies aimed at optimization of propionic acid production through biological routes, no efficient process that can be scaled up to an industrial level has been developed yet. Consequently, the goal of this project is the use of chemometric tools for the production of propionic acid from sugar cane syrup (concentrated sugar cane juice), taking into account three responses of extreme importance to an industry level process: yield, volumetric productivity and the response of lowest succinic acid formation (RMS). The first steps were the adaptation of a chromatography method for the quantification of sugars present in sugar cane syrup, the identification of strain P. acidipropionici ATCC 4875 as the most suitable organism for propionic acid production and the role of yeast extract as a nitrogen source in the culture medium. Using the experimental design method, developed by Plackett and Burman, 8 factors were evaluated to assess their importance in the process. Three of these eight factors (temperature, yeast extract concentration and sugar cane syrup concentration) were selected because of their significant influence on propionic acid production. In a second step, central composite design (CCD) was used to determine the optimum values of these factors, being 35°C for temperature, 22 g/L of yeast extract and 54 g/L of sugar cane syrup. Under these conditions, a yield of 47% (w/w), a volumetric productivity of 0.89 g/L. h and a RSM of 94% were obtained. These results represented an increase of 5%, 196% and 8% in yield, volumetric productivity and RMS, respectively, compared to the initial culture condition
Mestrado
Bioquimica
Mestre em Biologia Funcional e Molecular
Gardner, Nancy. "Production d'acide propionique sur un milieu riche en extraits de levure." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0006/MQ40583.pdf.
Full textCarpentier, Jean. "Variabilité moléculaire et mode évolutif du gène de virulence Gp-Rbp-A et du co-Facteur RanGap2 impliqués dans l’interaction incompatible entre le nématode Globodra pallida et la pomme de terre Gpa2 résistante." Rennes, Agrocampus Ouest, 2012. http://www.theses.fr/2012NSARC105.
Full textIn order to control the potato cyst nematode. Globodera pallida, using resistant varieties is now advocated. Nevertheless, most of the resistance genes, including ther major gene Gpa2 are efficient only against a lim ited number of nematode populations. Moreover Gpa2 needs the presence of a co-factor – RanGAP2 – to recognize the nematode avirulence protein coded by the Gp-RBP-1 gene and to trigger the plant defence mechanisms. The present work aims to characterize the efficiency spectrum of Gpa2. Our goals were to identify the Gp-Rbp-1 polymorphisms affecting the outcome of the interaction with Gpa2 and the polymorphisms in RanGAP2 that can be used to expand the range of G. Pallida populations controlled by Gpa2. We have shown tha susceptibility of a potato cultivar expressing Gpa2 to a G. Pallida population. Furthermore, among the eight sites of Gp-Rbp-1 found under positive selection, the sole variation at amino acid position 187 (proline/serine) remained sufficienet to explain the recognition of GP-RBP 1 by GPA2. Despite numerous sites found to have evolved under purifying selection, RanGAP2 have two polymorphic sites (amino acids 106 and 237) and one insertion/deletion of interest. Variability observed at these sites do not enable the recognition of virulent variants (non-recognized) of GP-RBP-1 by GPA2 but seems to affect intensity of the hypersensitive response triggered by the recognition of avirulent variants of Gp Rbp-1 GPA2
Aubin, Guillaume. "Phylogénie et tropisme de Cutibacterium acnes : impact sur la réaction inflammatoire et la réaction immunitaire lors des infections sur implant." Thesis, Nantes, 2017. http://www.theses.fr/2017NANT1006/document.
Full textUsing a Caenorhabditis elegans model, we identified two virulence groups of C. acnes not linked to their CC or clinical origin. Studies about C. acnes and bone cells interactions showed that CC36 C. acnes strains were significantly less internalized by osteoblasts and osteoclasts than CC18 and CC28 C. acnes strains. The CC18 C. acnes ATCC6919 isolate could survive intracellularly for at least 96 hours. C. acnes significantly decreased the resorption ability of osteoclasts with a major impact by the CC36 strain. Genome analysis revealed 27 genes possibly linked to these phenotypic behaviors. Studies about C. acnes interactions with immune cells revealed, thanks to a granuloma in vitro model, that isolate belonging to CC36 recruits more CD4+ lymphocytes inside the granuloma, whereas isolates belonging to CC18/28 recruit more CD4+ lymphocytes. The S8 strain isolated from the lymph node of a sarcoidosis patient generates more granulomas than other strains (acne and PJI isolates). Clinical strains of C. acnes resistant to rifampicin allowed us to characterize the mutations in the rpoB gene leading to this resistance. During this work, we identified and described a new species among the Actinobacteria phylum: Propionibacterium namnetense. Taken together, these studies provide new explanations about the development of C. acnes infections through the angle of the phylogeny of this bacteria. The aim of this thesis was to study the physiopathology of Cutibacterium (formerly Propionibacterium) acnes infections. From a phylogeny point of view, by MLST, we showed that C. acnes belonging to clonal complexes (CC) 36/53 were associated to prosthetic joint infections (PJI) and CC18/28 C. acnes isolates were linked to acne and spine material infections
Oprica, Cristina. "Characterisation of antibiotic-resistant Propionibacterium acnes from acne vulgaris and other diseases /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-755-3/.
Full textSutcliffe, Iain C. "The lipids and lipoglycan of Propionibacterium freudenreichii and other gram-positive bacteria." Thesis, University of Newcastle Upon Tyne, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328137.
Full textMcCafferty, Darragh. "Understanding the role of Propionibacterium acnes in the aetiology of prostate cancer." Thesis, Queen's University Belfast, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678939.
Full textDe, Canha Marco Nuno. "Antimicrobial and anti-inflammatory effect of southern African plants against Propionibacterium acnes." Diss., University of Pretoria, 2014. http://hdl.handle.net/2263/79786.
Full textDissertation (MSc)--University of Pretoria, 2014.
National Research Foundation (NRF)
Plant Science
MSc
Unrestricted
Dherbécourt, Julien. "Lipolyse dans le fromage : implication des enzymes de Propionibacterium freudenreichii dans l’emmental." Rennes, Agrocampus Ouest, 2008. http://www.theses.fr/2008NSARI049.
Full textLipolysis consists in the hydrolysis of fat by lipolytic esterases, usually named lipases, resulting in the subsequent release of free fatty acids. These fatty acids are essential aroma compounds in the flavour of numerous cheeses, including the economically important emmental cheese, in France. Propionibacteriuim freudenreichii a bacterial species used for ripening, plays a major role in the lipolysis of emmental cheese. However its direct involvement by action of its enzyme in this phenomenon remains to be confirmed. Moreover, esterase equipment of P. Freudenreichii was poorly documented. The aim of this PhD thesis was to identify the esterases of P. Freudenreichii,involved in the lipolyisis of emmental cheese. We elaborated the following strategy : (i) to characterise the lipolytic potential of the CIP103027T strain in pure culture and during ripening of emmental cheese, (ii) to seek as exhaustively and pertinently as possible, the putative esterases in the recently sequenced genome of this strain, by an innovative genomic approach we developed, and (iii) to determine, which of these putative esterases are the most susceptible to be involved in the lipolysis of emmental cheese. We confirmed that P. Freudenreichii is the main lipolytic agent in emmental cheese and showed that its role in lipolysis results from the direct action of one or more lipolytic esterases. We highlighted, in pure culture and in emmental cheese, that at least one of these enzymes is able to hydrolyse triacylglycerols. However this(ese) esterase(s) seem(s) to shom a preference for monoacylglycerols and/or diacylglycerols and would be rejioselective. We formulated, then confirmed the hypotheis that P. Freudenreichii possesses at least one extracellular esterase, involved in the lipolysis of emmental cheese. In the genome of CIP103027T strain, we identified 12 putative esterase genes
Johnson, Jessica Virginia. "Development of a Microbial Fuel Cell Cocatalyst with Propionibacterium freudenreichii ssp. shermanii." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38450.
Full textMerk, Kathrin. "In-vivo-Porphyrinproduktion von Propionibacterium acnes unter systemischer Aknetherapie mit Isotretinoin und Minozyklin." Diss., lmu, 2004. http://nbn-resolving.de/urn:nbn:de:bvb:19-31015.
Full textWalker, Michelle. "The prevention of spoilage in fruit juices by alicyclobacillus acidoterrestris and propionibacterium cyclohexanicum." Thesis, University of Northampton, 2006. http://nectar.northampton.ac.uk/2664/.
Full textSampaio, Romildo Martins. "Estudo da produção de vitamina B12 por bacterias dos generos propionibacterium e pseudomonas." [s.n.], 1999. http://repositorio.unicamp.br/jspui/handle/REPOSIP/255441.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: O presente trabalho trata do estudo da produção de vitamina B12, consistindo das seguintes etapas: seleção do melhor microrganismo produtor; seleção da melhor fonte de substrato, e do meia de cultura, avaliação, modelagem e otimização, das variáveis experimentais e fermentação em reator de bancada, para a obtenção das condições de operação e parâmetros cinéticos que influenciam no rendimento de produção da vitamina. A primeira fase consistiu da seleção do melhor microrganismo produtor a ser utilizado, nos estudos posteriores. Partiu-se das seguintes culturas tidas como boas produtoras de B12: Propionibacterium jensenii DSM 20274, Propionibacterium freudenreichii ATCC 9614, Propionibacterium shermanii ATCC 62Q1, Pseudomonas sp. ATCC 13867 e mais três mutantes isolados. Aqui como nas duas próximas etapas, os ensaios, foram realizados em frascos agitados, com base nos resultados, obtidos, decidiu-se pela seleção do mutante Pseudomonas P3, que chegou a uma produção máxima de 3,86 mg B12/1. A escolha da melhor fonte de substrato e do meio de cultura com composição mais indicada ocorreu paralelamente à escolha do microrganismo. Foram testadas lactose e sacarose e mais três composições de meio: meio 1, meio 2, e meio 3. A sacarose e o meio 1, associados ao mutante selecionado, proporcionaram os melhores rendimentos de B12, produção de biomassa e consumo de substrato. As culturas de. Propionibacterium mostraram maior um maior consumo de lactose do que as Pseudomonas. Na terceira etapa, estudou-se a influência de sete variáveis experimentais, previamente pesquisadas e selecionadas, na produção de B12. Foram elas: idade do inoculo, tempo de fermentação, temperatura, pH, concentração de substrato, de 5,6 DMI e de cobalto. Até a etapa de otimização, foram efetuados um planejamento fracionário, dois planejamentos com composto central e um caminho de ascendência máxima. As variáveis com seus respectivos níveis otimizados, que mais influenciaram a resposta, foram: concentração de 5,6 DMI (98,8 mg/l), temperatura (34,6 °C) e pH (7,14). O planejamento experimental permitiu um aumento de quase. 100% na produção de vitamina, alcançando um máximo de 7,57 mg BI2/1. Os ensaios no fermentador de bancada objetivaram verificar a influência do tempo de adição de 5,6 DMI, KLa e controle de pH no rendimento da vitamina. Concluiu-se que o tempo, ótima de adição, do precursor e a nível de Kls mais, indicado, foram respectivamente, 48 h e 36,8 h-1. Por outro lado, percebeu-se que o controle do pH não melhorou, a produção de vitamina nem aumentou, a velocidade de crescimento do microrganismo
Abstract: The present deals with the study of the production of vitamin B12, consisting of the following stages: selection of the best producing microorganism, selection of the best substrate, source, and the more, suitable medium of culture; evaluation, modeling and optimization of the experimental variables and; fermentation in a bench-fermentor for the obtaining of the operation, conditions and. kinetic parameters that, influence, the yield of the vitamin. The first phase consisted of the selection of the best producer microorganism to be used during the work. The following strains were used: Propionibacterium freudenreichii AICC 9614, Propiombacleruan jensenii. DSM 20274, Propianibacterium shermanii ATCC 6207, Pseudomonas sp. ATCC 13867 and more three mutants isolated. Here as in o stages, the experiments were, conducted in shake-flasks Based on the obtained results, the mutant Pseudomonas P3 was selected, producing a maximum of 3.86 mg BJ2/L. The selection of the best, substrate source, and medium composition was done parallelly to the microorganism selection. Were tested sucrose, lactose and three compositions of medium: medium 1 medium 2 and medium 3. The sucrose and medium 1, associated to the mutant P. P3, provided the best yields of B12, biomass production and substrate consumption. The strains of Propionibacterium showed larger ability in the lactose degradation than Pseudomonas and its mutants. In the third stage it was studied the influence of seven experimental variables in the production of vitamin B12: age of inoculum, time of fermentation, temperature, pH, substrate concentration, 5,6 Dimethylbenzimidazole (DMT> concentration and cobalt concentration. Up to the optimization step were realized a fractional factorial design, a steepest ascent path and two central composite design The most significant parameters, with respective optimized levels, were: 5,6 DMI (98.8 mg/L), temperature (34.6 °C) and pH (7-14)-. The experimental design allowed an. increase of almost 100% in. the Vitamin production, reaching a maximum of 7.57 mg B12/L. The experiments in the bench-fermentor were done to verify, the influence of the time of addition of 5,6 DMI, Kla and pH control in the yield of vitamin B12. The better time of addition and level of KLa, were respectively 48 hr and 36.8 hr-1. The control of pH did not improve the vitamin production
Doutorado
Doutor em Engenharia de Alimentos
Zhang, An. "Metabolic Engineering and Process Development for Enhanced Propionic acid Production by Propionibacterium acidipropionici." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1230919153.
Full textBergh, Drott Johanna. "The role of microorganisms in prostate cancer development." Doctoral thesis, Umeå universitet, Institutionen för klinisk mikrobiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-59987.
Full textBOKOBZA, PHILIPPE. "Infections neuromeningees a p. Acnes : a propos de 13 cas dont 9 porteurs de derivations de liquide cephalo-rachidien : etude des concentrations minimales inhibitrices de 8 antibiotiques sur ces souches." Reims, 1991. http://www.theses.fr/1991REIMMO97.
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