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Academic literature on the topic 'Propionibacterium'

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Published: 4 June 2021
Last updated: 26 January 2023

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Journal articles on the topic "Propionibacterium"

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CHAIA, ADRIANA PEREZ, AIDA PESCE de RUIZ HOLGADO, and GUILLERMO OLIVER. "Peptide Hydrolases of Propionibacteria: Effect of pH and Temperature." Journal of Food Protection 53, no. 3 (March 1, 1990): 237–40. http://dx.doi.org/10.4315/0362-028x-53.3.237.

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A comparative study of the activity of peptidases belonging to the four classic propionibacteria species cultured in milk was carried out at different values of pH and temperature. Leucine aminopeptidase and proline iminopeptidase showed greater activity in Propionibacterium freudenreichii than in the other species studied. With the single exception of Propionibacterium jensenii, the propionibacteria peptidase tested exhibited greater affinity for proline than for leucine-p-nitroanilide. Optimum temperature and pH in relation to the activity of both substrates varied according to the species under consideration.
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Șandru, Daniela Maria, and Magda Panaitescu. "THE ANTIBACTERIAL POTENTIAL OF ESSENTIAL OILS ON PROPIONIBACTERIUM GRANULOSUM STRAINS." Management of Sustainable Development 12, no. 2 (December 1, 2020): 21–24. http://dx.doi.org/10.54989/msd-2020-0008.

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Gram-positive bacteria Propionibacterium granulosum lives on human skin along with other propionibacteria on the skin, these bacteria are especially important to have healthy skin and occupy some ecological niches. These niches are populated by some pathogenic bacteria. Propionibacterium granulosum bacteria produce some fatty acids that have low molecular weight, bacteriocins and other substances that inhibit some bacteria. The aim of this research is to investigate the microbiological evolution of some essential oils on Propionibacterium granulosum strains. It is observed that the highest values are recorded when 20 µL of essential oil is used.
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Vyas, D., A. Alazzeh, S. M. McGinn, T. A. McAllister, O. M. Harstad, H. Holo, and K. A. Beauchemin. "Enteric methane emissions in response to ruminal inoculation of Propionibacterium strains in beef cattle fed a mixed diet." Animal Production Science 56, no. 7 (2016): 1035. http://dx.doi.org/10.1071/an14801.

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The objective of this study was to test the efficacy of Propionibacterium strains to mitigate enteric methane (CH4) emissions in beef heifers fed a mixed diet. An experiment was conducted with 16 ruminally cannulated beef heifers fed a basal diet consisting of 60 : 40 barley silage : barley grain (DM basis). Treatments included: (1) Control, (2) Propionibacterium freudenreichii T114, (3) P. thoenii T159, and (4) P. freudenreichii T54. Strains (1 × 1011 colony forming units) were administered daily directly into the rumen before feeding. No treatment effects were observed for DM intake (P = 0.90), mean ruminal pH (P = 0.50) and total volatile fatty acids (P = 0.44). However, compared with the Control, proportions of individual volatile fatty acids changed with acetate being less with Propionibacterium T159 (P = 0.02), whereas ruminal isobutyrate (P < 0.01) and acetate : propionate ratio (P = 0.04) were greater with Propionibacterium T114. Total daily enteric CH4 production averaged 188 g/day and was not affected by Propionbacterium strains (P = 0.51). Methane yield averaged 22 g/kg of DMI intake and tended to be greater with Propionibacterium strains (P = 0.08). The relative abundance of total Propionibacteria was greater with the inoculation of Propionibacterium T159 relative to the Control heifers (P = 0.04). In conclusion, inoculation of Propionibacterium T159 decreased ruminal acetate proportion and Propionibacterium T114 increased acetate : propionate ratio. However, inoculated strains failed to lower total CH4 emissions possibly due to the inability of Propionibacterium strains to elevate ruminal propionate concentrations.
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Farrar, Mark D., Karen M. Howson, Richard A. Bojar, David West, James C. Towler, James Parry, Katharine Pelton, and Keith T. Holland. "Genome Sequence and Analysis of a Propionibacterium acnes Bacteriophage." Journal of Bacteriology 189, no. 11 (March 30, 2007): 4161–67. http://dx.doi.org/10.1128/jb.00106-07.

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ABSTRACT Cutaneous propionibacteria are important commensals of human skin and are implicated in a wide range of opportunistic infections. Propionibacterium acnes is also associated with inflammatory acne vulgaris. Bacteriophage PA6 is the first phage of P. acnes to be sequenced and demonstrates a high degree of similarity to many mycobacteriophages both morphologically and genetically. PA6 possesses an icosahedreal head and long noncontractile tail characteristic of the Siphoviridae. The overall genome organization of PA6 resembled that of the temperate mycobacteriophages, although the genome was much smaller, 29,739 bp (48 predicted genes), compared to, for example, 50,550 bp (86 predicted genes) for the Bxb1 genome. PA6 infected only P. acnes and produced clear plaques with turbid centers, but it lacked any obvious genes for lysogeny. The host range of PA6 was restricted to P. acnes, but the phage was able to infect and lyse all P. acnes isolates tested. Sequencing of the PA6 genome makes an important contribution to the study of phage evolution and propionibacterial genetics.
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DARILMAZ, DERYA ONAL, and YESIM GUMUSTEKIN. "Research on Some Factors Influencing Acid and Exopolysaccharide Produced by Dairy Propionibacterium Strains Isolated from Traditional Homemade Turkish Cheeses." Journal of Food Protection 75, no. 5 (May 1, 2012): 918–26. http://dx.doi.org/10.4315/0362-028x.jfp-11-510.

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In this study, a total of 32 isolated strains and 5 reference strains of dairy propionibacteria were analyzed for acid and exopolysaccharide (EPS) production in skim milk and yeast extract–lactate broth (YEL) media in order to investigate the physiological background and preservative role of acid and EPS. The effects of final culture pH and optical density on acid and EPS production were also determined. On average, all strains produced more acid and reached lower final pH values in skim milk than in YEL medium. While the correlations obtained between the acid produced by propionibacterium strains and their final culture pH in skim milk medium were significant (P &lt; 0.01), no correlations were found between optical density, final pH, and produced acid in YEL medium. Sixteen isolated and five reference strains of propionibacteria were tested further for the ability to produce propionic and acetic acids. On average, Propionibacterium freudenreichii subsp. shermanii and P. freudenreichii subsp. freudenreichii strains produced higher amounts of propionic and acetic acids than did Propionibacterium jensenii in YEL medium. The acid produced by these strains may be used as a preservative in the food industry for replacement or reduction of the increasing use of chemical additives. The EPS production by propionibacterium strains during growth in YEL medium was 72 to 168 mg/liter, while in skim milk it was 94 to 359 mg/liter. The monomer compositions of the EPSs formed by the six selected dairy propionibacteria strains were analyzed. The EPSs may have applications as food grade additives and viscosity-stabilizing agents.
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CHAIA, A. PEREZ, A. M. STRASSER DE SAAD, A. PESCE DE RUIZ HOLGADO, and G. OLIVER. "Competitive Inhibition of Propionibacterium acidipropionici by Mixed Culturing with Lactobacillus helveticus." Journal of Food Protection 57, no. 4 (April 1, 1994): 341–44. http://dx.doi.org/10.4315/0362-028x-57.4.341.

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Lactobacillus helveticus and Propionibacterium acidipropionici were grown in pure and mixed cultures in a complex medium to assess the associative interaction. The specific growth rates, substrate consumption coefficient, substrate utilization and product formation rates were determined in each case. Propionibacterium acidipropionici utilized glucose preferably when it grew in a medium containing a mixture of glucose and lactate. Its growth rate was higher on glucose than on lactate in pure culture. However, lactic acid was the substrate utilized by propionibacteria in the associative growth. The fast pH reduction produced by the growth of lactobacilli and the slow lactate utilization by propionibacteria in mixed culture determined the inhibition of propionic acid bacteria in associative growth.
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Faye, Therese, Thor Langsrud, Ingolf F. Nes, and Helge Holo. "Biochemical and Genetic Characterization of Propionicin T1, a New Bacteriocin from Propionibacterium thoenii." Applied and Environmental Microbiology 66, no. 10 (October 1, 2000): 4230–36. http://dx.doi.org/10.1128/aem.66.10.4230-4236.2000.

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ABSTRACT A collection of propionibacteria was screened for bacteriocin production. A new bacteriocin named propionicin T1 was isolated from two strains of Propionibacterium thoenii. This bacteriocin shows no sequence similarity to other bacteriocins. Propionicin T1 was active against all strains of Propionibacterium acidipropionici, Propionibacterium thoenii, andPropionibacterium jensenii tested and also againstLactobacillus sake NCDO 2714 but showed no activity againstPropionibacterium freudenreichii. The bacteriocin was purified, and the N-terminal part of the peptide was determined with amino acid sequencing. The corresponding gene pctA was sequenced, and this revealed that propionicin T1 is produced as a prebacteriocin of 96 amino acids with a typical sec leader, which is processed to give a mature bacteriocin of 65 amino acids. An open reading frame encoding a protein of 424 amino acids was found 68 nucleotides downstream the stop codon of pctA. The N-terminal part of this putative protein shows strong similarity with the ATP-binding cassette of prokaryotic and eukaryotic ABC transporters, and this protein may be involved in self-protection against propionicin T1. Propionicin T1 is the first bacteriocin from propionibacteria that has been isolated and further characterized at the molecular level.
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Jamil, Khanssa Badie. "Synthesis of Environmental Bioplastic Polyhydroxyalkanoate (PHA) from Waste Glycerol, Palm Oil and Different Concentrations of Glucose by A New Strain Propionibacterium Sp." Iraqi Journal of Industrial Research 9, no. 2 (October 20, 2022): 175–86. http://dx.doi.org/10.53523/ijoirvol9i2id203.

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Propionibacteria have mainly been found in dairy products and on the skin of humans and animals. This genus classified as non-producing PHA but in this study we show that Propionibacterium sp. can produce PHA. A member of the genus Propionibacterium was isolated from soil. The isolate was identified based on its gram positive pleomorphic rods. Cells occur short chains, singly or in pairs, in V or Y configurations. On mineral salt agar colonies are semi-opaque, convex, glittering and often pigmented in orange in color, biochemical tests and 16SrDNA also done. The 16SrDNA analysis confirmed 85% identity to Propionibacterium (accession number NR 074675.1). The presence of 3HB was identified, based on the analysis of NMR. 13C and 1H NMR analysis confirmed that Propionibacterium sp was able to produce PHA.polymer. These confirm the results of GC. The isolate was then grown on media with waste glycerol (WG), palm oil, different concentration of glucose and one stage and two stages of cultivation. This lead to the conclusion that Propionibacterium is able to grow utilizing waste glycerol, palm oil and different concentration of glucose as the sole carbon source under limited conditions. The PHA content 3 (wt%) and the composition (mol%) and (86% 3HB) when used glucose 2% at frist stage cultivation. while the PHA content 13 (wt %) when used palm oil and 5 (wt%) when utilized (WG), as sole carbon source.
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McCubbin, Tim, R. Axayacatl Gonzalez-Garcia, Robin W. Palfreyman, Chris Stowers, Lars K. Nielsen, and Esteban Marcellin. "A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity." Genes 11, no. 10 (September 23, 2020): 1115. http://dx.doi.org/10.3390/genes11101115.

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Propionibacteria have been studied extensively since the early 1930s due to their relevance to industry and importance as human pathogens. Still, their unique metabolism is far from fully understood. This is partly due to their signature high GC content, which has previously hampered the acquisition of quality sequence data, the accurate annotation of the available genomes, and the functional characterization of genes. The recent completion of the genome sequences for several species has led researchers to reassess the taxonomical classification of the genus Propionibacterium, which has been divided into several new genres. Such data also enable a comparative genomic approach to annotation and provide a new opportunity to revisit our understanding of their metabolism. Using pan-genome analysis combined with the reconstruction of the first high-quality Propionibacterium genome-scale metabolic model and a pan-metabolic model of current and former members of the genus Propionibacterium, we demonstrate that despite sharing unique metabolic traits, these organisms have an unexpected diversity in central carbon metabolism and a hidden layer of metabolic complexity. This combined approach gave us new insights into the evolution of Propionibacterium metabolism and led us to propose a novel, putative ferredoxin-linked energy conservation strategy. The pan-genomic approach highlighted key differences in Propionibacterium metabolism that reflect adaptation to their environment. Results were mathematically captured in genome-scale metabolic reconstructions that can be used to further explore metabolism using metabolic modeling techniques. Overall, the data provide a platform to explore Propionibacterium metabolism and a tool for the rational design of strains.
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Moroder, Philipp, Andrej Trampuz, and Markus Scheibel. "Propionibacterium." Journal of Bone and Joint Surgery 98, no. 24 (December 2016): e112. http://dx.doi.org/10.2106/jbjs.16.00838.

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Dissertations / Theses on the topic "Propionibacterium"

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Perry, Alexandra L. "Characterisation of propionibacterium acnes." Thesis, Aston University, 2004. http://publications.aston.ac.uk/11015/.

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This thesis has sought to investigate the phenotypic, genetic and antigenic properties of P. acnes strains isolated from sciatica patients undergoing microdiscectomy, normal skin, blood cultures, prosthetic hips and acne lesions. Isolates’ phenotype was examined by determining their biotype by analytical profile index, antimicrobial susceptibility, virulence factor expression and serotype. A molecular typing method for P. acnes was developed using random amplification of polymorphic DNA (RAPD). Patent serum was used to screen P. acnes strains for antigens expressed in vivo and the chemical composition determined. The serodiagnostic potential and inflammatory properties of identified antigens were assessed. The optimised and reproducible RAPD protocol classified strains into three major clusters and was found to distinguish between the serotypes I and II for a large number of clinical isolates. Molecular typing by RAPD also enabled the identification of a genotype that did not react with the type I or II monoclonal antibodies and these strains may therefore constitute a previously undiscovered subspecies of P. acnes with a genetic background different from the type I and II serotypes. A major cell-associated antigen produced by all strains as identified and characterised. A serological assay based on the antigen was used to measure IgG and IgM levels in serum from patients with acne, sciatica and controls. No difference in levels of antibodies was detected. Inflammatory properties of the antigen were measured by exposing murine macrophage-like cells and measuring the release of nitric oxide and tumour necrosis factor-alpha (TNF-a). Only TNF-a was elicited in response to the antigen. The phenotypic, genotypic and antigenic properties of this organism may provide a basis for future studies on P. acnes virulence and provide an insight into its mechanisms of pathogenesis.
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Glenn, J. V. "Propionibacterium acnes and medical device infection." Thesis, Queen's University Belfast, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273034.

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Valanne, M. S. "The pathogenic potential of Propionibacterium acnes." Thesis, Queen's University Belfast, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273147.

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Fougnot, Sébastien Lozniewski Alain. "Etude de l'activité des antibiotiques sur propionibacterium acnes impliqué dans les infections neuro-méningées." [S.l] : [s.n], 2003. http://www.scd.uhp-nancy.fr/docnum/SCDMED_T_2003_FOUGNOT_SEBASTIEN.pdf.

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Lan, Annaïg. "Survie et potentialités probiotiques de Propionibacterium freudenreichii." Rennes 1, 2006. http://www.theses.fr/2006REN1S125.

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Afin d’étudier les potentialités probiotiques de P. Freudenreichii, une bactérie propionique laitière principalement étudiée pour ses propriétés technologiques en industrie fromagère, plusieurs souches ont été sélectionnées par criblages in vitro puis in vivo pour tester leur survie et leurs activités métaboliques dans le tractus digestif. Une des souches, présentant des aptitudes probiotiques in vitro et in vivo, a été administrée à des rats à microbiote humain afin de tester son influence sur la carcinogenèse colique à court et à moyen termes. Cette souche s’est révélée être protectrice dans les stades précoces de carcinogenèse en augmentant significativement l’apoptose des cellules épithéliales coliques mais est sans effet sur la réduction du nombre de lésions prénéoplasiques coliques. Par ailleurs, les scénarii létaux induits par les métabolites des propionibactéries in vitro ont été identifiés et précisés en tenant compte du gradient de pH, variable physiologique intestinale.
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Fachin, Luciano. "Contagem de Bifidobacterium animalis Bb 12 e efeito da adição de Propionibacterium freudenreichii PS-1 e do tratamento termico do leite sobre o desenvolvimento de Bifidobacterium animalis Bb 12 em iogurte." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/255805.

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Orientadores: Walkiria Hanada Viotto, Mieko Kimura
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
Made available in DSpace on 2018-08-04T02:47:09Z (GMT). No. of bitstreams: 1 Fachin_Luciano_D.pdf: 623874 bytes, checksum: 39038138708fcb9d94eb3e5320272cbb (MD5) Previous issue date: 2005
Resumo: A produção de iogurtes com Bifidobacterium spp. tem crescido muito nas últimas décadas visando a produção de alimentos funcionais. Contudo, para que o produto possa apresentar tais propriedades, tem sido alegado que o número mínimo de células viáveis de Bifidobacterium spp. presentes no momento do consumo deva ser de 106 UFC/g de produto. Entretanto, vários estudos têm mostrado produtos comerciais com contagens menores do que as recomendadas, durante a estocagem do iogurte. Esse fato é reflexo tanto da dificuldade de incorporação destes microrganismos ao iogurte, devido às condições de processo que não são favoráveis ao desenvolvimento da bifidobactéria, bem como pela dificuldade de enumeração destes microrganismos na presença das culturas do iogurte. Este trabalho avaliou o uso dos meios M-MRS, MRS-NNLP, MRS-LP, RCPB pH5 e RCPB pH5 enriquecido com extrato de fígado, visando a contagem seletiva ou diferencial de Bifidobacterium animalis Bb 12 na presença das culturas do iogurte e estudou o efeito do tratamento térmico do leite, visando o aumento do teor de lactulose e, da adição de Propionibacterium freudenreichii PS-1, sobre o desenvolvimento e manutenção do número de células viáveis de B. animalis Bb 12 durante a fermentação e estocagem do iogurte. Dos meios estudados, o meio RCPB pH5, enriquecido com 150mL/L de extrato de fígado, foi o mais indicado para a contagem de B. animalis Bb 12 em iogurte por ter apresentado uma excelente diferenciação deste microrganismo, após a estocagem refrigerada do iogurte. O tratamento térmico do leite de 142°C/15 segundos não afetou o desenvolvimento de B. animalis Bb 12 durante a fermentação do iogurte e também não influenciou a sua resistência à estocagem refrigerada. Entretanto, o tratamento térmico alterou significativamente a textura, diminuindo consideravelmente a dureza, gomosidade e adesividade do iogurte, resultando em um produto com menor separação de soro. A adição de P. freudenreichii PS-1 aumentou em aproximadamente duas vezes o número de células de B. animalis Bb 12 ao final da fermentação e melhorou a resistência da bifidobactéria à estocagem refrigerada. A presença da propionibactéria também alterou significativamente a textura final do iogurte, aumentando consideravelmente a gomosidade e adesividade do produto final, bem como resultou em um iogurte com menor separação de soro durante a estocagem refrigerada
Abstract: Bifidobacterium spp. has been used to produce probiotic yoghurts due to its therapeutic properties. However, it has been claimed that the number of bifidobactéria in yoghurt at the time of consuming must be 106 CFU /g of product to perform their therapeutic functions. Many studies found a low recovery of bifidobactéria from commercial products during shelf life. The low viability of bifidobactéria can be attributed to the process conditions of yoghurt, which is not favorable to the growth of bifidobactéria, and to the difficulty for enumeration of bifidobactéria in the presence of yoghurt bacteria. The objectives of this work were to evaluate the following media: M-MRS, MRS-NNLP, MRS-LP, RCPB pH5 and fortified RCPB pH5 to enumerate B. animalis Bb 12 in yoghurt, and to evaluate the heat treatment of milk (142°C/15 seconds) and the addition of P. freudenreichii PS-1 on the growth of B. animalis Bb 12 during yoghurt fermentation and during shelf life. RCPB pH5 fortified with 150 mL/L of liver extract was the best media due to its excellent differentiation during refrigerated storage of yoghurt. The heat treatment of milk (142°C/15 seconds) did not have effect on the growth of bifidobactéria during fermentation and it also did not improve the bifidus viability during storage. Heat treatment, however, had a strong effect on yoghurt texture, decreasing the sineresis and some parameters of TPA (hardness, gumminess and adhesiveness). The addition of P. freudenreichii PS-1 increased two fold the bifidobactéria growing during yoghurt fermentation and it also increased the viability of bifidobactéria during yoghurt shelf life. The addition of P. freudenreichii PS-1 also decreased the sineresis and increased TPA parameters of hardness, gumminess and adhesiveness. Addition of P. freudenreichii PS-1 to the yoghurt seems to be a good growth promoter for B. animalis Bb 12
Doutorado
Tecnologia de Alimentos
Doutor em Tecnologia de Alimentos
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Curiche, Natalia. "Visualization of Propionibacterium acnes in Patients Diagnosed with Acne Vulgaris. - Propionibacterium acnes Detected with Immuno­fluorescence and Fluorescence in situ Hybridization." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-58623.

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Van, der Merwe Iansha (Iansha Rosalia) 1975. "Characterization of thoeniicin 447 produced by Propionibacterium thoenii." Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52729.

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Thesis (MSc)--University of Stellenbosch, 2002.
ENGLISH ABSTRACT: Antimicrobial peptides continue to be one of the most important classes of food additives. The food industry is especially interested in the application of naturally occuring and biologically derived preservatives. Among the metabolites of industrial importance produced by propionibacteria are peptides called bacteriocins. Bacteriocins are ribosomally synthesized peptides with antagonistic activity against closely related microorganisms. Many microorganisms associated with food produce bacteriocins, which have stimulated interest in the use of these peptides as natural food preservatives. Numerous bacteriocins are produced by lactic acid bacteria, but only a few have been reported for propionibacteria. Since propionic acid bacteria have GRAS (generally regarded as safe) status, their metabolic compounds should be safe for human consumption. Propionibacterium thoenii 447, isolated from Emmentaler cheese, produces a bacteriocin-like peptide, named thoeniicin 447, with a narrow spectrum of activity. The peptide displays a bactericidal mode of action against Lactobacillus delbrueckii subsp. bulgaricus and a bacteriostatic action against Propionibacterium acnes. Optimal bacteriocin production was detected during the early stationary growth phase. The peptide is resistant to heat treatments of 60°C and 80°C for 15 and 30 min and to 100°C for 15 min, but loses 80% of its activity after autoclaving (10 min at 121°C). Thoeniicin 447 remains active after incubation in buffers with pH values ranging from 1-10. The peptide is inactivated by pepsin, pronase, a-chymotrypsin, trypsin and Proteinase K. Thoeniicin 447 was partially purified by ammonium sulfate precipitation, followed by SP-Sepharose cation exchange chromatography. The estimated size of thoeniicin 447, according to tricine-SDSPAGE, is approximately 6 kDa. Based on DNA sequencing, the mature peptide is 7130 Da in size and homologous to propionicin Tl produced by P. thoenii strain 419. Thoeniicin 447 is a relatively small, cationic and heat-stable peptide and can therefor be classified as a member of class II bacteriocins. These features are very similar to those of bacteriocins produced by lactic acid bacteria. However, no unique classification system has been proposed for bacteriocins of propionibacteria. As a member of the genus Propionibacterium, P. thoenii 447 is generally regarded as safe. This, together with the narrow spectrum of activity, particularly the action against P. acnes, heat tolerance of thoeniicin 447 and its activity over a wide pH range renders the peptide suitable for possible pharmaceutical applications.
AFRIKAANSE OPSOMMING: Antimikrobiese middels sal deurgaans beskou word as een van die belangrikste klasse van voedsel bymiddels. Die voedselindustrie is veral geïnteresseerd in die toepassing van preserveermiddels van 'n meer natuurlike en biologiese oorsprong. Onder die metaboliese produkte van industriële belang wat deur propionibakterieë geproduseer word is antimikrobiese peptiede (bakteriosiene). Bakteriosiene is ribosomaal-gesintetiseerde peptiede met 'n antagonistiese aktiwiteit teenoor naverwante bakterieë. Verskeie bakteriosiene word deur melksuurbakterieë geproduseer, terwyl slegs enkele vir propionibakterieë beskryf is. Baie van hierdie propionibakterieë word in die algemeen as veilig beskou en het GRAS status. Die metaboliete wat hulle produseer behoort dus veilig vir menslike gebruik te wees. Propionibacterium thoenii 447 is uit Emmentaler kaas geisoleer en produseer 'n bakteriosien-agtige peptied, naamlik thoeniicin 447 met 'n beperkte spektrum van aktiwiteit. Die peptied het 'n bakteriosidiese werking teenoor Lactobacillus delbrueckii subsp. bulgaricus en 'n bakteriostatiese werking teenoor Propionibacterium acnes. Optimum bakteriosien produksie is verkry tydens die vroeë stationêre groeifase. Die peptied is bestand teen hittebehandelings van 60°C en 80°C vir 15 en 30 min, asook 100°C vir 15 min, maar verloor 80% van sy aktiwiteit na outoklavering (lOmin by 121°C). Die peptied blyaktief na inkubasie in buffers van pH 1-10. Die peptied word deur pepsien, pronase, uchymotripsien, tripsien en Proteinase K geïnaktiveer. Thoeniicin 447 is met behulp van ammoniumsulfaat-presipitasie, gevolg deur SPSepharose katioon-uitruilchromatografie gedeeltelik gesuiwer. Skeiding op "n trisien-SDS poliakrielarnied-jel het 'n aktiewe band van ongeveer 6 kDa getoon. Volgens die DNA volgorde bepaling is thoeniicin 447, 7130 Da in grootte en homoloog aan Propionicin Tl, geisoleer vanaf P. thoenii stam 419. Thoeniicin 447 is 'n relatiewe klein, kationiese en hitte-bestande peptied en kan op grond hiervan as 'n lid van die klas II bakteriosiene geklassifiseer word. Hierdie eienskappe is soortgelyk aan die eienskappe van bakteriosiene geproduseer deur melksuurbakterieë. Tot op hede is geen klassifikasiesisteem vir die bakteriosiene van propionibakterieë voorgestel nie. As 'n lid van die genus Propionibacterium, word P. thoenii 447 in die algemeen as veilig beskou. Dit, tesame met die nou spektrum van aktiwiteit, veral teenoor P. acnes, die hittetoleransie van thoeniicin 447, asook die aktiwiteit oor 'n wye pH-grens, maak die peptied geskik vir moontlike farmaseutiese toepassings.
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9

Grassi, Maria Carolina de Barros 1984. "Estudo genético e metabólico da bactéria Propionibacterium acidipropionici." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316784.

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Orientadores: Gonçalo Amarante Guimarães Pereira, Johana Rincones Perez
Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Doutorado
Genetica de Microorganismos
Doutor em Genetica e Biologia Molecular
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10

Vieira, Machado Eduardo Souza. "Culture de propionibacterium freudenreichii et production d'arôme emmental." Compiègne, 1994. http://www.theses.fr/1994COMP734S.

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La biomasse de propionibacterium freudenreichii a été préparée en vue de la production d'arome emmental. Dans une première partie nous avons étudié l'effet de l'aération, de l'agent de neutralisation et de l'addition d'extrait de levure sur la croissance des bactéries propioniques cultivées sur lactoserum proteolyse a 32c et ph 6,8. Les résultats obtenus démontrent que la souche répond favorablement à une faible aération (1,0 vvm/200 rpm), et la vitalité mesurée en termes de ufc/ml passe de 0,2 a 1,2. 10#1#0 ufc/ml. Des conditions d'aération plus énergiques sont nuisibles pour les cellules. L'utilisation de nh#4oh a la place de naoh a permis d'augmenter de 11,4% la matière sèche de la culture et la vitalité en termes d'ufc/ml. L'hydroxyde de calcium n'a pas d'effet important sur le dénombrement maximal mais les cultures produites avec son utilisation sont plus stables. Une fois déterminées les conditions optimales, des cultures ont été conduites pour l'obtention de la biomasse pour la production de l'arome emmental sur caille granulaire. L'addition d'extrait de levure s'est montrée favorable à la production de biomasse et à la vitalité, mais réduit le rapport acide propionique/acide acétique des cultures. Cinq types de caille ont été testés, tout en variant les cultures d'inoculation: bactéries propioniques pures, et additionnées de l. Helveticus, s. Thermophilus ou mélangé des deux souches à 0,1% ou 1,0% de chaque souche lactique. Le meilleur caille a été obtenu après l'addition des souches lactiques a 0,1%. Ce caille soumis a un panel de dégustation a été considéré comme type et de force 5.
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Books on the topic "Propionibacterium"

1

Khamagaeva, I. S. Biotekhnologii Ła zakvasok propionovskikh bakterii. Ulan-Ude: Izd-vo VSGTU, 2006.

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Adlam, C. Structure and function of selected antigens from the pathogenic bacteria Propionibacterium acnes, Staphylococcus aureus and Pasteurella haemolytica. Birmingham: University of Birmingham, 1987.

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Vorobjeva, Lena I. Propionibacteria. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-017-2803-4.

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Vorobjeva, L. I. Propionibacteria. Springer, 2014.

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Hutchinson, Jonathan A. Antibiotic resistance in propionibacterium acnes. 1995.

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Vorobjeva, L. I. Propionibacteria. Springer, 1999.

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Vorobjeva, L. I. Propionibacteria. Springer, 2010.

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Salih, Mohammed Ali. Studies on growth metabolites produced by Propionibacterium shermanii. 1985.

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Vorobjeva, L. I. Propionibacteria. Springer London, Limited, 2013.

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Teo, Alex Yeow-Lim. Effects of propionibacterial metabolites on spoilage and pathogenic bacteria in dairy products. 1993.

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Book chapters on the topic "Propionibacterium"

1

Johnson, Douglas I. "Propionibacterium spp." In Bacterial Pathogens and Their Virulence Factors, 121–25. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-67651-7_8.

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Vorobjeva, Lena I. "The genus Propionibacterium." In Propionibacteria, 4–46. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-017-2803-4_2.

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Bährle-Rapp, Marina. "Propionibakterie, auch: Propionibacterium." In Springer Lexikon Kosmetik und Körperpflege, 454. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_8531.

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Stackebrandt, Erko, Cecil S. Cummins, and John L. Johnson. "Family Propionibacteriaceae: The Genus Propionibacterium." In The Prokaryotes, 400–418. New York, NY: Springer New York, 2006. http://dx.doi.org/10.1007/0-387-30743-5_19.

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Brüggemann, H. "Skin: Acne and Propionibacterium acnes Genomics." In Handbook of Hydrocarbon and Lipid Microbiology, 3215–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-77587-4_244.

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Stackebrandt, Erko. "The Family Propionibacteriaceae: Genera other than Propionibacterium." In The Prokaryotes, 725–41. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-30138-4_194.

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Clark, J. E. "Purification of Polyphosphate Glucokinase from Propionibacterium Shermanii." In Novel Biodegradable Microbial Polymers, 213–21. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-2129-0_18.

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Brüggemann, Holger. "Skin: Cutibacterium (formerly Propionibacterium) acnes and Acne Vulgaris." In Health Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids, 225–43. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-15147-8_20.

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Brüggemann, Holger. "Skin: Cutibacterium (formerly Propionibacterium) acnes and Acne Vulgaris." In Health Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids, 1–20. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-72473-7_20-1.

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Patrick, Sheila, and Andrew McDowell. "Propionibacterium acnes: An Emerging Pathogen in Biomaterial-Associated Infection." In Biomaterials Associated Infection, 87–105. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-1031-7_4.

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Conference papers on the topic "Propionibacterium"

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Koenig, Karsten, M. Teschke, Stephen G. Eick, W. Pfister, Herbert Meyer, and Karl-Juergen Halbhuber. "Photodynamic-induced inactivation of Propionibacterium acnes." In BiOS '98 International Biomedical Optics Symposium, edited by Thomas J. Dougherty. SPIE, 1998. http://dx.doi.org/10.1117/12.308137.

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Tuchina, Elena S., and Valery V. Tuchin. "In vitro LED and laser light photoinactivation of Propionibacterium acnes." In SPIE Proceedings, edited by Valery V. Tuchin. SPIE, 2008. http://dx.doi.org/10.1117/12.803986.

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Zhang, Yongguang, Yin Liu, Fan Zhang, Rubing Zhang, and Jianhang Zhu. "The effect of ethanol on the growth and propionic acid formation of propionibacterium acidipropionici." In 2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2010. http://dx.doi.org/10.1109/bmei.2010.5639469.

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Kusuma, Irawan Wijaya, Rahmini, Enih Rosamah, Supomo, and Harlinda Kuspradini. "Antibacterial Activity Against Propionibacterium acnes of n-Hexane Fractions from Siam Weed Leaves (Chromolaena odorata)." In Joint Symposium on Tropical Studies (JSTS-19). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/absr.k.210408.033.

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Meinisasti, Resva, Wenti Puspita, and Raden Sunita. "Test Effectiveness Antimicrobial Extract Etanol Leaves Melinjo (Gnetum gnemon L.) On Growth Of Bacteria Propionibacterium Acnes." In Proceedings of the 1st International Conference on Inter-professional Health Collaboration (ICIHC 2018). Paris, France: Atlantis Press, 2019. http://dx.doi.org/10.2991/icihc-18.2019.25.

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Weng, D., J. Song, and H. Li. "The Role of IL-17A in the Mouse Model of Sarcoid Granulomatous Mice Induced by Propionibacterium Acnes." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a4504.

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Enwemeka, Chukuka, Violet Bumah, Daniela Masson-Meyers, Dawn Castel, and Chris Castel. "Optimizing the antimicrobial efficacy of pulsed 450-nm light on Propionibacterium acnes through correlation with fluorescence spectroscopy." In Photonics in Dermatology and Plastic Surgery 2019, edited by Bernard Choi and Haishan Zeng. SPIE, 2019. http://dx.doi.org/10.1117/12.2510796.

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Tuchina, Elena S., and Valery V. Tuchin. "Low-intensity LED (625 and 405 nm) and laser (805 nm) killing of Propionibacterium acnes and Staphylococcus epidermidis." In SPIE BiOS: Biomedical Optics, edited by Michael R. Hamblin, Ronald W. Waynant, and Juanita Anders. SPIE, 2009. http://dx.doi.org/10.1117/12.814812.

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Desrini, Sufi, and Hifzhan Maulana Ghiffary. "Comparison of antibacterial activity of Talok (Muntingia calabura L) leaves ethanolic and n-hexane extracts on Propionibacterium acnes." In PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON APPLIED SCIENCES (ICAS-2). Author(s), 2018. http://dx.doi.org/10.1063/1.5033382.

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Fitri, Noor, Ifat Fatimah, Lutfi Chabib, and Febi Indah Fajarwati. "Formulation of antiacne serum based on lime peel essential oil and in vitro antibacterial activity test against Propionibacterium acnes." In PROCEEDINGS FROM THE 14TH INTERNATIONAL SYMPOSIUM ON THERAPEUTIC ULTRASOUND. Author(s), 2017. http://dx.doi.org/10.1063/1.4978196.

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Reports on the topic "Propionibacterium"

1

Jain, M. K., R. Narayan, and O. Han. Anaerobic bioprocessing of low-rank coals. [Veillonella alcalescens and Propionibacterium acidipropionici]. Office of Scientific and Technical Information (OSTI), January 1992. http://dx.doi.org/10.2172/5520833.

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Barefoot, Susan F., Bonita A. Glatz, Nathan Gollop, and Thomas A. Hughes. Bacteriocin Markers for Propionibacteria Gene Transfer Systems. United States Department of Agriculture, June 2000. http://dx.doi.org/10.32747/2000.7573993.bard.

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The antibotulinal baceriocins, propionicin PLG-1 and jenseniin G., were the first to be identified, purified and characterized for the dairy propionibaceria and are produced by Propionibacterium thoenii P127 and P. thoenii/jensenii P126, respectively. Objectives of this project were to (a) produce polyclonal antibodies for detection, comparison and monitoring of propionicin PLG-1; (b) identify, clone and characterize the propionicin PLG-1 (plg-1) and jenseniin G (jnG) genes; and (3) develop gene transfer systems for dairy propionibacteria using them as models. Polyclonal antibodies for detection, comparison and monitoring of propionicin PLG-1 were produced in rabbits. Anti-PLG-1 antiserum had high titers (256,000 to 512,000), neutralized PLG-1 activity, and detected purified PLG-1 at 0.10 mg/ml (indirect ELISA) and 0.033 mg/ml (competitive indirect ELISA). Thirty-nine of 158 strains (most P. thoenii or P. jensenii) yielded cross-reacting material; four strains of P. thoenii, including two previously unidentified bacteriocin producers, showed biological activity. Eight propionicin-negative P127 mutants produced neither ELISA response nor biological activity. Western blot analyses of supernates detected a PLG-1 band at 9.1 kDa and two additional protein bands with apparent molecular weights of 16.2 and 27.5 kDa. PLG-1 polyclonal antibodies were used for detection of jenseniin G. PLG-1 antibodies neutralized jenseniin G activity and detected a jenseniin G-sized, 3.5 kDa peptide. Preliminary immunoprecipitation of crude preparations with PLG-1 antibodies yielded three proteins including an active 3-4 kDa band. Propionicin PLG-1 antibodies were used to screen a P. jensenii/thoenii P126 genomic expression library. Complete sequencing of a cloned insert identified by PLG-1 antibodies revealed a putative response regulator, transport protein, transmembrane protein and an open reading frame (ORF) potentially encoding jenseniin G. PCR cloning of the putative plg-1 gene yielded a 1,100 bp fragment with a 355 bp ORF encoding 118 amino acids; the deduced N-terminus was similar to the known PLG-1 N-terminus. The 118 amino acid sequence deduced from the putative plg-1 gene was larger than PLG-1 possibly due to post-translational processing. The product of the putative plg-1 gene had a calculated molecular weight of 12.8 kDa, a pI of 11.7, 14 negatively charged residues (Asp+Glu) and 24 positively charged residues (Arg+Lys). The putative plg-1 gene was expressed as an inducible fusion protein with a six-histidine residue tag. Metal affinity chromatography of the fused protein yielded a homogeneous product. The fused purified protein sequence matched the deduced putative plg-1 gene sequence. The data preliminarily suggest that both the plg-1 and jnG genes have been identified and cloned. Demonstrating that antibodies can be produced for propionicin PLG-1 and that those antibodies can be used to detect, monitor and compare activity throughout growth and purification was an important step towards monitoring PLG-1 concentrations in food systems. The unexpected but fortunate cross-reactivity of PLG-1 antibodies with jenseniin G led to selective recovery of jenseniin G by immunoprecipitation. Further refinement of this separation technique could lead to powerful affinity methods for rapid, specific separation of the two bacteriocins and thus facilitate their availability for industrial or pharmaceutical uses. Preliminary identification of genes encoding the two dairy propionibacteria bacteriocins must be confirmed; further analysis will provide means for understanding how they work, for increasing their production and for manipulating the peptides to increase their target species. Further development of these systems would contribute to basic knowledge about dairy propionibacteria and has potential for improving other industrially significant characteristics.
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Barefoot, Susan, Benjamin Juven, Thomas Hughes, Avraham Lalazar, A. B. Bodine, Yitzhak Ittah, and Bonita Glatz. Characterization of Bacteriocins Produced by Food Bioprocessing Propionobacteria. United States Department of Agriculture, August 1992. http://dx.doi.org/10.32747/1992.7561061.bard.

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Objectives were to further characterize activity spectra of dairy propionibacteria bacteriocins, jenseniin G and propionicin PLG-1, purify them, examine the role of cell walls in resistance, examine their interactions with cytoplasmic membrane, explain producer immunity, and clone the responsible genes. Inhibitory spectra of both bacteriocins were further characterized. Propionicin was most effective in controlling Gram-positive, rather than Gram-negative organisms; it controlled growth of sensitive cells both in a culture medium and a model food system. Jenseniin inhibited yogurt cultures and may help prevent yogurt over-acidification. Both were active against botulinal spores; jenseniin was sporostatic; propionicin was sporicidal. Jenseniin was produced in broth culture, was stable to pH and temperature extremes, and was purified. Its molecular mass (3649 Da) and partial amino acid composition (74%) were determined. A blocked jenseniin N-terminus prevented sequencing. Methods to produce propionicin in liquid culture were improved, and large scale culture protocols to yield high titers were developed. Methods to detect and quantify propionicin activity were optimized and standardized. Stability of partially purified propionicin was demonstrated and an improved purification scheme was developed. Purified propionicin had a 9328-Da molecular mass, contained 99 amino acids, and was significantly hydrophobic; ten N-terminal amino acids were identified. Propionicin and Jenseniin interacted with cytoplasmic membranes; resistance of insensitive species was cell wall-related. Propionicin and jenseniin acted similarly; their mode of action appeared to differ from nisin. Spontaneous jenseniin-resistant mutants were resistant to propionicin but nisin-sensitive. The basis for producer immunity was not resolved. Although bacteriocin genes were not cloned, a jenseniin producer DNA clone bank and three possible vectors for cloning genes in propionibacteria were constructed. In addition, transposon Tn916 was conjugatively transferred to the propionicin producer from chromosomal and plasmid locations at transfer frequencies high enough to permit use of Tn916 for insertional mutagenesis or targeting genes in propionibacteria. The results provide information about the bacteriocins that further supports their usefulness as adjuncts to increase food safety and/or quality.
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