Dissertations / Theses on the topic 'Prolactin'
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Bernard, Valérie. "Rôle de la prolactine dans la tumorigenèse du prolactinome." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS280.
Full textIn this work, we investigated the role of prolactin (PRL) in prolactinoma tumorigenesis. We first described the natural and molecular history of lactotroph cell tumors developed by the Prlr-/- mouse model, globally invalidated for the PRL receptor (PRLR). The Prlr-/- females develop prolactinomas with 100% penetrance at 12 months of age. These tumors are highly secreting, invasive and proliferative. The comparative transcriptomic analysis of pituitaries from Prlr+/+ and Prlr-/- mice suggested new signaling pathways involved in lactotroph adenoma developement in this mouse model. The role of these novel candidate genes remains to be demonstated in Humans. Furthermore, by studying another mouse model developed during this work, deleted for Prlr only in lactotroph cells, we demonstrated for the first time that PRL exerts an autocrine feedback on lactotroph cell secretion and proliferation in vivo. Although we did not find any germline mutation of PRLR in a large cohort of patients with sporadic prolactinoma, our results suggest that somatic mutations of this gene cannot be excluded and may contribute to the onset of the human pathology
Gordon, Timothy Jason. "THE BIOLOGICAL, STRUCTURAL AND KINETIC PROPERTIES OF PROLACTIN, PROLACTIN RECEPTOR ANTAGONISTS, GROWTH HORMONE AND THE PROLACTIN RECEPTOR." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1366356833.
Full textUeda, Eric Kinnosuke Martins. ""Prolactina humana pseudofosforilada (S179D-hPRL) é um potente fator anti-angiogênico in vitro e in vivo"." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-28052007-162443/.
Full textS179D-prolactin (hPRL) is an experimentally useful mimic of naturally phosphorylated human prolactin. S179D-hPRL, but not unmodified PRL, was found to be anti-angiogenic in both the chorioallantoic membrane and corneal assays. Further investigation using human endothelial in vitro models showed reduced cell number, reduced tubule formation in Matrigel, and reduced migration and invasion, as a function of treatment with S179D-hPRL. Analysis of growth factors in human endothelial cells in response to S179D-hPRL showed a decreased expression or release of endogenous PRL, heme-oxygenase-1, basic fibroblast growth factor (bFGF), angiogenin, epidermal growth factor and vascular endothelial growth factor and an increased expression of inhibitors of matrix metalloproteases. S179D-hPRL also blocked signaling from bFGF in these cells. We conclude that this molecular mimic of a pituitary hormone is a potent anti-angiogenic protein, partly as a result of its ability to reduce utilization of several well-established endothelial autocrine growth loops, partly by its ability to block signaling from bFGF and partly because of its ability to decrease endothelial migration. We also examined the influence of S179D-hPRL on apoptosis in human endothelial cells, using procaspase-8 as a marker of the extrinsic pathway, and cytochrome C release as a marker of the intrinsic pathway. Both pathways converge at caspase-3, which cleaves DNA fragmentation factor (DFF45). A 3-day incubation with 50 ng/ml S179D-hPRL quadrupled the early apoptotic cells; this effect was doubled at 100 ng/ml and maximal at 500 ng/ml. DFF45 and pro-caspase 8 cleavage were detectable at 100 ng/ml. Cytochrome C, however, was unaffected until 500 ng/ml. p21 increased at 100 ng/ml, whereas a change in p53 activity required both triple the time and 500 ng/ml. p21 promoter activity was maximal at 50 ng/ml, whereas 500 ng/ml were required to see a significant change in the Bax promoter (a measure of p53 activity). As previously shown, S179D-hPRL blocked extracelular regulated kinase (ERK) phosphorylation in response to bFGF, but, in addition, continued co-incubation showed a delayed and prolonged activation of ERK. PD98059 [a specific mitogen-activated protein kinase (MAPkinase) inhibitor] inhibited this delayed activation of ERK and the effects of S179D-hPRL on all parameters except p53, or activity of the Bax promoter. We conclude that low doses of S179D-hPRL block bFGF-induced ERK signaling and yet activates ERK in a different time frame to elevate p21, and activate the extrinsic pathway. Longer incubations and higher concentrations, however, additionally activate the intrinsic pathway using an alternate intracellular signal. These findings suggest that circulating levels of phosphorylated hPRL may reduce the progression of cancer and, furthermore, that S179D-hPRL may be a useful anti-angiogenic therapeutic.
Furigo, Isadora Clivatti. "Estudo do mecanismo de ação da bromocriptina e de antagonistas de prolactina no tratamento do Diabetes Mellitus tipo 2 e da obesidade." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/42/42137/tde-16052017-145647/.
Full textType 2 Diabetes mellitus (T2DM) is a syndrome characterized by dysfunctions in the metabolism of glucose, amino acids and free fat acids. Although most of the drugs currently used to treat T2DM targets peripheral organs, a growing interest in studying the Central Nervous System (CNS) as a potential target of antidiabetic drugs is appearing. The CNS possesses insulin receptors and plays a critical role in regulating glucose homeostasis. In this sense, Cycloset® (quick release bromocriptine mesylate) a drug that acts on CNS, was recently approved in United States to treat T2DM. Previous studies have shown beneficial effects of bromocriptine (Bromo) on hyperglycemia and hyperlipidemia in obese animal models. As a dopaminergic agonist, a possible mechanism of action of this drug could be caused by a decreased prolactin (Prl) production and release. High serum prolactin levels, as observed in patients bearing prolactinomas or individuals using drugs that induce hyperprolactinemia, generate abnormalities in carbohydrate and lipid metabolism, which can lead to metabolic syndrome. In the current thesis, we tested the hypothesis that part of bromocriptine antidiabetic effects is due to an inhibition of prolactin secretion. We evaluated Bromo effects in genetically obese and insulin resistant male and female mouse (ob/ob), as well as we tested whether replacing Prl could reverse the beneficial effects of Bromo. Males treated with Bromo showed lower insulin resistence, whereas Prl replacement decreased insulin sensitivity. Females treated with Bromo showed tendency towards an improvement in their insulin sensitivity and glucose tolerance. Prl replacement also reversed the beneficial effects of Bromo in this group. Thus, we demonstrated that at least part of the antidiabetic effects of Bromo is due to inhibition of Prl secretion. In another set of experiments, we tested whether central or peripheral treatment with prolactin antagonists (G129R-hPrlR) causes antidiabetic effects in ob/ob male mice. Both peripheral and central treatment decreased the glycemic curve during glucose and insulin tolerance tests, although we still did not obtain statistically significant values with our sample size. Lastly, we investigated whether metabolic Prl action occurs due to a putative interaction with estrogen receptor alpha (ERα). We found a wide co-expression between Prl receptor and ERα in the CNS. Additionally, changes in estrogen levels decrease prolactin sensitivity. Therefore, in the present study we identified the possible mechanism by which bromocriptine promotes improvements in glycemic control, and for the first time, we obtained evidence that the use of prolactin antagonists can have a potential effect in the treatment of T2DM.
Amaral, Vinícius Cestari do. "Expressão gênica da prolactina e seus receptores na hipófise e no útero de camundongo fêmea tratado com metoclopramida." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/5/5139/tde-18092012-114713/.
Full textINTRODUCTION: Prolactin is a polypeptide hormone with a recognized systemic action mainly on reproductive physiology. However, prolactin imbalance, particularly hyperprolactinemia, is increasingly more frequent in clinical practice. Although it is a comparatively common disorder, there are still doubts about the molecular effects of hyperprolactinemia on the genital tract especially in the uterus and the pituitary. The present study aimed at verifying the effects of metoclopramide-induced hyperprolactinemia on the gene expression of prolactin and its receptors in the uterus and pituitary of the female mouse. METHODS: Forty-nine female Wistar mice were randomized to 7 equal-sized groups as follows: 1) SS nonoophorectomized mice treated with saline solution (vehicle); 2) M nonoophorectomized mice treated with metoclopramide; 3) OSS oophorectomized mice treated with saline solution (vehicle); 4) OM oophorectomized mice treated with metoclopramide; 5) OME oophorectomized mice treated with metoclopramide and 17-estradiol; 6) OMP oophorectomized mice treated with metoclopramide and micronized progesterone; 7) OMEP oophorectomized mice treated with metoclopramide, 17-estradiol, and micronized progesterone. The animals were sacrificed 50 days after the end of the treatment, and the uterus and pituitary of each animal were removed for extraction of total ribonucleic acid, which was then used for synthesizing complementary deoxyribonucleic acid and for evaluating the gene expression of prolactin and the different isoforms of its receptors by the real-time polymerase chain reaction. RESULTS: In the pituitary of the nonoophorectomized mice, the treatment with metoclopramide against that with vehicle alone increased the expression of the prolactin-encoding gene. In the castrated animals, progesterone by itself or in conjunction with estrogen determined a raise in prolactin messenger RNA as opposed to the two other treatments with different combinations. This effect was similar to that produced by metoclopramide in animals with intact ovaries. Estrogen and progesterone, acting independently of each other, were responsible for the increase in the S2 isoform of the prolactin receptor. In the uterus, there was heightened expression of prolactin messenger RNA under the effect of the treatment with metoclopramide or with estrogen and/or progesterone. Oophorectomy caused a greater reduction in expression of the prolactin receptor S1 and S2 isoforms than in the other isoforms. However, the combined estrogen plus progesterone treatment led to an increase in the S3 and L forms of the receptor, while progesterone alone resulted solely in a higher expression of the L form of the prolactin receptor in the endometrium of the castrated mice. CONCLUSION: Our data suggest that metoclopramide treatment induces different changes in the expression of prolactin and its receptors according to whether the effect occurs in the pituitary or the uterus of castrated female mice treated with sex steroids
Patmastan, Piyanuj. "Comparison of properties of wild-type human prolactin and a potent antagonist." Columbus, OH : Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1061248268.
Full textTitle from first page of PDF file. Document formatted into pages; contains xv, 163 p.: ill. (some col.). Includes abstract and vita. Advisor: Charles L. Brooks, Dept. of Veterinary Biosciences. Includes bibliographical references (p. 156-163).
Gould, David R. (David Ross). "Prolactin in human breast cancer." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41006.
Full textAmaya, Julieta Esperanza Ochoa. "Efeitos da hiperprolactinemia sobre a inflamação alérgica pulmonar em ratos Wistar." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/10/10133/tde-09052016-143242/.
Full textObjective: It was investigated if hyperprolactinemia has modulatory actions on lung allergic inflammatory response in male rats. Lactating female rats were tested for pulmonary allergy as well. Methods: In male rats, short-term (5 days) hyperprolactinemia was induced by domperidone (5.1 mg.kg-1 per day, ip). Allergic response was generated by sensitization and inhalation challenge with ovalbumin. Total and differential leukocytes bronchoalveolar lavage (BAL), femoral medullary lavage (BFL) and blood; the percentage of collagen and mucus production in the lungs, plasma levels of corticosterone and prolactin cytokines and TNF-α, IL-4, IL-6, IL-10, IFNg explants lung and BAL, were measured. Flow cytometry was used to evaluate prolactin receptor; Results: Short-term hyperprolactinemia made before the inhaled challenge reduced the pulmonary allergic response in white blood cell counts in BAL. This treatment reduced the cellularity in BFL and the percentage of mucus and increased expression of cytokines IL-4, IL-6, IL-10, TNFa and IFNg expression. High prolactin levels decreased the number of eosinophils to the lung in BAL. There were fewer granulocytes migrated to the lung. These granulocytes showed higher expression prolactin receptors in hyperprolactinemia animals. Similar changes were revealed in lactating females. In these animals, there was a reduction in BAL leukocyte, and the number of cells BFL. Prophylactic treatment decreased the allergic response in both hyperprolactinemic and vehicle groups. The treatment made after inhalational challenge did not induce significant changes in the variables measured in this study. Conclusions: Short-term hyperprolactinemia, made after sensitization and before inhalation, decreases the inflammatory response in the lung of rats. The results of this study demonstrate that hyperprolactinemia, induced before antigen challenge, decreases pulmonary allergic inflammation. Thus, it is probable that the endogenous prolactin has an important role as an immunomodulator of asthma. This study points out the prospect of a future use of domperidone for asthmatic patients. For various mammalian species, parturition occurs during springtime. Pollen in the air might be an abundant allergic factor during springtime. Thus, protecting lactating females against this type allergy might have high adaptive value
Arthuso, Fernanda dos Santos. "Adaptação de células CHO secretoras de prolactina humana e seus antagonistas para o crescimento em suspensão." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-20062011-110202/.
Full textThe Hormone Group of the Biotechnology Center of IPEN has developed different cells lines of genetically modified chinese hamster ovary cells (CHO) for the expression of heterologus protein like human prolactin (hPRL) and its analogs/antagonists (S179D-hPRL and G129R-hPRL). All cell lines for expression are however cultured in monolayer culture dish and depend on fetal bovine serum (FBS) in the medium for an efficient growth. Cells in suspension show a great industrial-pharmaceutical interest, especially for the cultivation facility and scale enlargement as well as for volumetric productivity. We developed a protocol for adapting CHO cells to suspension growth, in spinner flasks. The adaption of our cell lines producing hPRL; S179D-hPRL and G129R-hPRL to suspension growth and in serum-free medium was obtained. We also carried out laboratory scale production with the three suspension-adapted culture line cells and the corresponding purification and characterization of four heterologous proteins, including glycosylated human prolactin (G-hPRL).
Constantino, Flávia Bessi. "Relativa influência da prolactina, sua inibição e combinação com testosterona sobre a próstata de ratos castrados comparação entre recrescimento glandular, diferenciação celular e atividade secretora /." Botucatu, 2017. http://hdl.handle.net/11449/148973.
Full textResumo: A próstata é uma glândula do sistema genital masculino que possui grande importância na fertilidade. Estudos clássicos evidenciaram que para que se inicie o desenvolvimento prostático é necessário a presença de andrógeno produzido pelos testículos fetais. Porém, além da estimulação androgênica, estudos apontam para outros hormônios que também atuam na próstata como a Prolactina (PRL). PRL é um hormônio que é principalmente secretado por células lactotróficas da hipofise anterior e está envolvido em muitos processos biológicos, incluindo lactação e reprodução. Assim, investigamos se a modulação da sinalização PRL altera a morfofisiologia da próstata ventral (VP) em ratos castrados. Os ratos Sprague Dawley adultos (n = 6) foram castrados e após 21 dias divididos em 10 grupos experimentais: Castrado Controle (CC): animais castrados que não receberam tratamento; Castrado+testosterona (T): animais castrados que receberam T (4mg / kg); Castrado+PRL (PRL): animais castrados recebendo PRL (0,3 mg / kg); Castrado+T+PRL (TPRL): animais castrados que receberam associação de T e PRL; e Castrado+BR (BR): animais castrados que receberam Bromocriptina (BR) (0,4mg / kg). Grupo Controle: animais intactos (CTR). Os animais foram tratados durante 3 ou 10 dias consecutivos. Ao fim dos tratamentos, os animais foram anestesiados, eutanizados e a prostata ventral (VP) foi removida, pesada e processada para análise histológica e Western blot. O peso corporal não se alterou entre os grupos experiment... (Resumo completo, clicar acesso eletrônico abaixo)
Mestre
Glezer, Andrea. ""Estudo da atividade biológica da macroprolactina humana em células Nb2 e em células Ba/F-03 transfectadas com o receptor de prolactina humano forma longa"." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-12042006-085305/.
Full textMacroprolactinemia is a frequent finding in hyperprolactinemic individuals, usually without clinical impact. Data on biological activity of macroprolactin (bbPRL) is mostly based on a heterologous bioassay (Nb2 cell). Biological activity of bbPRL observed in vitro but not in vivo maybe due to its high molecular weight preventing its passage through capillary barrier. Alternatively, bbPRL bioactivity may differ depending on the PRL receptor species specificity. BbPRL from macroprolactinemic individuals and monomeric PRL (mPRL) from hyperprolactinemic patients without macroprolactinemia were tested in two bioassays: Nb2 and in Ba/F-LLP, which expresses human prolactin receptor. While both bioassays achieve similar results considering mPRL activity, our results indicate that bbPRL displays activity in a heterologous but not in a homologous bioassay, consistently with the apparent absence of bbPRL bioactivity in vivo
Albinsson, Agneta. "Serotonin receptors in the regulation of prolactin release and some behaviors in the rat." Lund : Dept. of Zoophysiology, University of Lund, 1995. http://catalog.hathitrust.org/api/volumes/oclc/38865664.html.
Full textBédécarrats, Grégoy. "Studies on variants of prolactin in the turkey." Thesis, McGill University, 1999. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=35854.
Full textA competitive reverse transcription polymerase chain reaction assay was developed in order to semi-quantify the PRL mRNA in individual pituitary glands from turkey embryos and poults. Pituitary content and plasma levels of PRL were also monitored, and the PRL isoforms present in the pituitary gland were detected. The levels of PRL mRNA remained low until five days before hatching, increased until the day of hatch, plateaued during the first three days of age and significantly increased at two weeks of age. Similar changes were observed in pituitary content and plasma concentrations of PRL which were highly correlated. Two immunoreactive bands corresponding to the NG- and G-PRL detected in adult pituitary gland were visualised on western blots. The percentage of G-PRL in pituitary glands were 31.5, 48.6, 48.0 and 56.2 at 22 and 27 days of incubation and at I and 7 days of age, respectively. Thus, higher percentages of G-PRL (27 kDa) were associated with higher levels of total PRL in the pituitary gland.
Suzuki, Miriam Fussae. "Síntese e caracterização de prolactina de camundongo (mPRL) e deu seu análogo (S177D-mPRL)." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-29062011-154905/.
Full textProlactin is a neurohormone included in cytokine superfamily and involved in innumerous biological processes. Due to its endocrine, autocrine and paracrine action, it is, frequently, related to development of human pathologies, such as carcinomas and autoimmune diseases. Considering some factors: a) the difference of 41% between the amino acid sequence of mouse prolactin in relation to the human one, b) glycosylation, phosphorylation and receptor ligation differences, and c) the fact that the animal model used for in vivo assays with human prolactin are generally rats or mice (heterologous), it is evident that these factors might interfere in the interpretation of results. Therefore, experiments with homologous systems are desirable. This work describes, for the first time, the expression of mouse prolactin in the periplasmic space of bacteria, in its authentic form, i. e., without initial metionine, showing expression levels of 0.1 ± 13.2% g/mL/A600. For this purpose, an expression vector based on PL promoter was constructed and used as a constitutive form, with activation at 37° C. A fermentation process in bioreactor, with expression yields up to 2.5 g/mL, and purification process with three steps: concentration and purification by hydrophobicity (Phenyl Sepharose CL-4B) followed by reverse phase HPLC and HPSEC, were also developed. The mPRL was purified and characterized by chemo physical and biological techniques compared to the recombinant standard reference from the National Institute of Health (NIH, USA). Its biological activity was analyzed and the calculated potency was 33.9 ± 1.4 UI/mg. The same vector was used for the expression of S177D-mPRL antagonist, but the low expression level obtained makes it impracticable to produce this protein in the periplasmic space of bacteria. As an alternative, the S177D-mPRL was produced in CHO cells and clones with expression level of about 1 g/mL/day were obtained. The expression of both proteins, the authentic mPRL and the S177D-mPRL, opens the door for developing studies with animal models if mPRL and its antagonist might be considered relevant factors.
Resalat-Panah, Nazila. "Characterization of Anp32 in Prolactin Signaling." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=114174.
Full textLa prolactin(PRL) et sa voie de signalisation en aval Jak2/Stat5a sont requies à la croissance de la glande mammaire, ainsi qu'à la différentiation des épithéliales mammaires. Cependant, le rôle de la PRL dans le développement du cancer du sein n'est pas encore clair. Quelques preuves récentes, obtenues par notre laboratoire, montrent que la PRL pourrait supprimer certains aspects de la carcinogenèse du sein. Par consequent, nous avons effectué une analyse de puce à ADN pour examiner les genes cibles de la en utilisant les cellules épithéliales mammaires de souris HC11. Sur la base de l'analyse micro-array, nous avons identifié la protèin Anp32a, une petite nuclèo- protein cytoplasmique acid comme une gene cible impliqué en aval de la voie de signalization de la PRL. Nous avons davantage confirmé ces résultats en utilisant la Reaction de polymerization en chaine (PCR) et l'analyse d'immunobuvardage de type western. En plus, nous avons examiné la contribution de cette protéine dans la régulation de la voie de signalisation de la PRL conduisant à l'activation de la voie Jak2/Stat5a. Les résultats ont montré que Anp32a est transloquée au noyau et deviant phosphorylée par la stimulation de la PRL. En plus, la kinase JAK2 est nécessaire pour la phosphorylation sur les tyrosines de Anp32a.De façon inattendue, nous avons constaté que la stimulation de la PRL conduit à la formation d'un complexe entre les protéines Anp32a/Stat5 dans les cellules épithéliales mammaires. En plus, nous avons confirmé cette interaction en observant la co-localisation par des études d'immunofluorescence par microscopie confocale. En outre, à l'aide des protéines de fusion de GST représentant différentes domains de Anp32 en utilisant des études d'association in vitro, nous avons illustré le fait que Stat5a nécessite les domains qui sont pré sent en N et C terminal. En parallèle, nous avons examiné le niveau d'expression de Anp32a dans les plusieurs lignées cellulaire de cancer du sein. Nos resultats preliminaries, présente un niveau d'expression réduite de Anp32a en cellules cancéreuse mammaires 4T1(hautement métastatiques) par rapport à les cellules épithéliales mammaires HC11.
歐惠連 and Wai-lin Au. "Molecular characterization of chicken prolactin gene." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31227119.
Full textSauvé, Danielle. "Neuroanatomical specificity of prolactin-induced hyperphagia and expression of Fos-like immunoreactivity following central administration of prolactin." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape9/PQDD_0001/NQ39020.pdf.
Full textBUCKLEY, ARTHUR RALPH JR. "EFFECTS OF PROLACTIN ON BIOCHEMICAL MARKERS OF HEPATIC PRENEOPLASIA IN THE RAT." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183834.
Full textPinaffi, Fábio Luís Valerio. "Dinâmica hormonal durante o processo luteolítico nas espécies equina e bovina; com ênfase sobre o papel da prolactina." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-16122013-113326/.
Full textThe aim of the present study was to characterize the PRL secretion and study the relationship between PRL and PGFM during preluteolysis, luteolysis and postluteolysis in mares (Experiment 1); evaluate the effect of PRL and PGF2α inhibition on luteolysis and define the synchrony between PRL and PGFM in heifers (Experiment 2); define the synchrony between PRL and PGFM in mares (Experiment 3); and evaluate the frequent stimulation of PRL during the estrous cycle in mares (Experiment 4). On experiment 1 in mares, blood samples were collected during the 24 h of preluteolysis, luteolysis and postluteolysis. Concentrations of PRL and PGFM were rhythmic. Prolactin pulses had 5h of duration, interval of 7,5 h between pulses, and 12 h between peaks. Pulses of PRL were more prominent during luteolysis and postluteolysis. Concentrations of PRL during PGFM pulses differ during luteolysis and postluteolysis, and were greater at the peak of PGFM. The synchrony between peaks of PRL and PGFM was greater during luteolysis and postluteolysis. On experiment 2 in heifers, the secretion of PRL and PGF2α were inhibited during luteolysis. The PRL inhibition was associated with greater concentrations of P4 and LH. The inhibition of PGF2α was associated with a decrease on PRL concentrations, but no effect on PGFM was observed after PRL inhibition. The CL area measurement was an efficient method to target luteolysis. On experiment 3 in mares, in summer and autumn, secretion of PGF2α and PRL were inhibited on Day 14. The inhibition of PGF2α reduced PGFM concentrations. No effect on PGFM was observed after PRL inhibition. Concentrations of PGFM were not different between summer and autumn, and PRL concentrations were low in the autumn. In the summer, PRL inhibition reduced PGF2α concentrations. On experiment 4 in mares, PRL was stimulated every 8 h. Blood samples were collected every 12 h from Day 13 to ovulation, and every hour for 12 h on Day 14. The frequent stimulation on PRL did not appear to maintain higher concentrations of PRL after Day 14. On hourly samples, concentrations of PRL reached maximum value 4 h after stimulation and pulses of PRL were increased. The increase on PRL did not affect PGFM, P4, and blood flow of the CL. The synchrony between PGFM and PRL was partially disrupted by PRL stimulation. This was the first report on characterization and rhythm of PRL pulses, synchrony between PRL and PGFM pulses, and greater PRL activity during luteolysis and postluteolysis. The inhibition of PRL interfered with P4 secretion in heifers, but was confounded by the LH increase. In mares and heifers, the synchrony between PGFM and PRL pulses represents a positive effect of PGF2α on PRL.
Sivaprasad, Umasundari. "The mechanism of lactogen receptor binding by human prolactin." Connect to this title online, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1054499303.
Full textTitle from first page of PDF file. Document formatted into pages; contains xiii, 133 p.; also includes graphics (some col.) Includes bibliographical references (p. 124-133). Available online via OhioLINK's ETD Center
Gaasenbeek, Michelle Lynn. "Regulation of non-pituitary prolactin gene expression." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ32480.pdf.
Full textZhou, Jiang Feng. "Characterization of prolactin receptor in Meleagris gallopavo." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0019/NQ44647.pdf.
Full textTurrone, Peter. "Prolactin response with typical and atypical antipsychotics." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0017/MQ54150.pdf.
Full textZhou, Jiang Feng 1964. "Characterization of prolactin receptor in meleagris gallopavo." Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=35661.
Full textRozakis-Adcock, Maria. "Structurefunction analysis of the rat prolactin receptor." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41027.
Full textHo, Ming-Kai 1978. "Characterization of glycosylation of prolactin in galliformes." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98724.
Full textLaPensee, Christopher Ryan. "METABOLIC FUNCTIONS OF PROLACTIN IN THE MOUSE." University of Cincinnati / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1172587296.
Full textChristensen, Heather R. "Molecular and Integrated Systems Physiology of Prolactin." University of Cincinnati / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1314040076.
Full textHyde, Jennie. "The Role of Prolactin in CCL28 Regulation." BYU ScholarsArchive, 2007. https://scholarsarchive.byu.edu/etd/1305.
Full textHui, Mei-yee Angela, and 許美儀. "Molecular characterization of the chicken prolactin receptor gene." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31228227.
Full textWang, Ying, and 王莹. "Molecular and functional characterization of the prolactin receptor, prolactin-releasing peptide receptor, and growth hormone-releasinghormone receptor genes in chicken." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39556864.
Full textOliveira, Taís Lima de. "Desenvolvimento de processo de fermentação em biorreator para produção de prolactina humana secretada no espaço periplásmico de Escherichia coli." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-07072009-152955/.
Full textProlactin (PRL) is one of the most versatile hormones in terms of biological action. His best known action is related to the stimulation of lactation and regulation of growth and differentiation of the mammary gland; it also has wide important diagnostic applications. Considering all the increasing studies on its potential therapeutic applications, the need for obtaining this hormone in its pure, biologically active and authentic form becomes clearer and clearer. The fundamental objective of this project was the production of hPRL on the laboratory scale, from genetically modified bacteria (E.coli), using an expression system based on Lambda () PL promoter, the same successfully used in our laboratory for the expression of hGH. We set up a cultivation process in bioreactor, where the repressor (cIts), a thermo-sensitive protein that is usually used to inhibit the PL promoter during the growth phase (30°C). The cultivation process presents basically three stages: the first step in was not used the growth is carried out without the continuous addition of nutrients (batch cultivation), the second step in which a continuous addition of nutrients and carbohydrate occurs (fed-batch cultivation) and a final step when activation is carried out. The latter is characterized by an increased temperature, still maintaining the addition of nutrients and carbohydrate. This fast and flexible process of fermentation, with the average duration of 20 hours, led to a final biomass of approximately 30 A600nm (units of optical absorbance at 600nm), with the expression of about 1g of hPRL mL-1A600 -1, the highest ever reported for the secretion of prolactin in the periplasmic space. Monomeric hPRL was purified and characterized by physical-chemical methods and biological assays, which confirmed its biological and immunological activity, correct processing and a relative molecular mass (Mr) of 22,906.
Gregory, Susan Jan. "The intrapituitary regulation of fertility in the seasonal breeder." Thesis, University of Bristol, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247177.
Full textAlmgren, Colleen Marie. "The potential role of potent prolactin antagonists as chemotherapeutics for human cancers an evaluation of select prolactin antagonists in human breast cancer cells /." Connect to this title online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1104778150.
Full textTitle from first page of PDF file. Document formatted into pages; contains xx, 199 p.; also includes graphics (some col.) Includes bibliographical references (p. 178-199).
Bédécarrats, Grégoy Yves. "Studies on variants of prolactin in the turkey." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0028/NQ50110.pdf.
Full textDiogenes, Anibal. "Prolactin modulation of trigeminal sensory neurons : a dissertation /." San Antonio : UTHSC, 2006. http://proquest.umi.com/pqdweb?did=1246523531&sid=1&Fmt=2&clientId=70986&RQT=309&VName=PQD.
Full textKatoh, Masao. "Biochemical and immunological characterization of the prolactin receptor." Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=73962.
Full textGrosser, Peter M. "Testosterone modulation of plasma gonadotropin and prolactin patterns." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75750.
Full textDowell, Kenneth. "Prolactin molecular heterogeneity in normoprolactinaemic and hyperprolactinaemic serum." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305105.
Full textGilbert, Mark Simon. "The expression of human prolactin in Escherichia coli." Thesis, University of Reading, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.238668.
Full textKurima, Kiyoto. "Transcriptional regulation of the prolactin gene in turkeys." Diss., Virginia Tech, 1996. http://hdl.handle.net/10919/37773.
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Hyde, Jennie. "The role of prolactin in regulating CCL28 expression /." Diss., CLICK HERE for online access, 2007. http://contentdm.lib.byu.edu/ETD/image/etd1717.pdf.
Full textVander, Hamm Dale Gene 1954. "Prolactin regulation of lymphocyte proliferation: An early event." Diss., The University of Arizona, 1998. http://hdl.handle.net/10150/282723.
Full textLarsen, Caroline, and n/a. "Pheromones, prolactin and maternal behavior : (male pheromones initiate prolactin-induced neurogenesis, decrease anxiety and advance maternal behavior in virgin female mice)." University of Otago. Department of Anatomy & Structural Biology, 2007. http://adt.otago.ac.nz./public/adt-NZDU20071019.134553.
Full textWang, Ying. "Molecular and functional characterization of the prolactin receptor, prolactin-releasing peptide receptor, and growth hormone-releasing hormone receptor genes in chicken." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39556864.
Full textHurst, Thomas Eugene. "Role of nociceptin/orphanin FQ in the prolactin, hypothalamic-pituitary-adrenal axis, and prolactin receptor response to acute stress in rats." Miami University Honors Theses / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1303421079.
Full textNguyen-Bresinsky, Dong Thi. "Immunopurification of Bovine Placental Lactogen." Fogler Library, University of Maine, 2005. http://www.library.umaine.edu/theses/pdf/Nguyen-Bresinsky2005.pdf.
Full textAlmgren, Colleen Marie D. V. M. Ph D. "The potential role of potent prolactin antagonists as chemotherapeutics for human cancers: an evaluation of select prolactin antagonists in human breast cancer cells." The Ohio State University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=osu1104778150.
Full textSilveira, Marina Augusto. "Controle neuroendócrino da reprodução: fatores que modulam a atividade de neurônios GNRH e kisspetina." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/42/42131/tde-16112017-175827/.
Full textGnRH and kisspeptina neurons represent the most important neuronal populations in the control of reproduction. Estradiol binds to its receptor expressed by the kisspeptina neurons to regulate the release of GnRH. In the animal model OVX+E the activity of the GnRH neuron and LH surge is dependent of estradiol and time of day. In this study, although the firing rate of GnRH neurons was similar between groups, the pattern of potentials revealed a change to longer burst duration in mice in proestrous, and the pituitary response was greater in this group. Prolactin has impact on HPG axis modulation and kisspeptin is a mediator of the effects of prolactin on reproduction. A small percentage of AVPV kisspeptin neurons were indirectly depolarized by prolactin. This effect required the PI3K signaling pathway. Mice bearing Stat5a/b inactivation on kisspeptin cells exhibited an early onset of estrus cyclicity, indicating that STAT5 transcription factors exert an inhibitory effect on the time of puberty.
Silva, Fábio Fernando Alves da. "Avaliação do papel de HSPB1 na modulação da autofagia induzida por PRL em células-beta." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-24082018-092045/.
Full textType 1 diabetes mellitus is a metabolic disease characterized by glycemic dysregulation, which occurs due to an autoimmune destruction of beta-cells. Insulin therapy is the gold standard treatment for DM1. However, some DM1 patients do not respond efficiently to this treatment and suffer frequent episodes of severe hypoglycemia unawareness. Since this complication jeopardizes the quality of life of these people, Islet transplantation is a therapeutic alternative indicated to treat these patients. However, besides the lack of enough organ donors, the loss of beta cells during both the isolation as well as the infusion of islets into the recipient induce a great estresse and thus a significant cell death is one of the drawbacks of this procedure. Autophagy is a mechanism of recycling cytoplasmic components and is essential for cellular homeostasis. Under estresse conditions, this mechanism is activated above basal levels, promoting the degradation of protein aggregates and defective organelles, thus avoiding cell damage that could compromise cell viability. Studies carried out by our group have shown not only that PRL promotes cytoprotection in beta-cells, reducing pro-inflammatory cytokines-induced apoptosis, but also that HSPB1 plays an essential role in this inhibition of apoptosis mediated by PRL after treatment with cytokines. Moreover, recent results from our laboratory showed an increase in autophagy levels in beta-cells after exposure to cytokines, as well as a restauration to normal levels in the presence of PRL. In order to better understand the role of PRL in the modulation of autophagy in these cells, the aim of this project is to study whether HSPB1 is also essential in the mechanism of autophagy regulation induced by PRL. Using MIN6 beta cell models where HSPB1 was silenced (MIN6-shHSPB1) or not (MIN6-SsC), we studied cell death by viability assays. Moreover, western blot assays were performed in order to assess levels of autophagy and autophagic flux markers in the cells.Our results showed that HSPB1 in one of the mediators of PRL-induced modulation of autophagy. Nevertheless, since hormonal treatment was still able to inhibit cytokinesinduced cell death even in the presence of chloroquin, an autophagy blocker, we conclude that autophagy is not a signaling pathway involved in PRl-induced beta-cell cytoprotection. Altogether, the results shown in this study may help to increase the knowledge of the molecular events induced by PRL in beta-cells, and may allow to infer new approaches to improve cytoprotection, culture and transplantation of these cells into type 1 diabetic patients.