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Academic literature on the topic 'Process cultivation concentration starters'

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Published: 30 May 2022
Last updated: 31 May 2022

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Journal articles on the topic "Process cultivation concentration starters"

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Boonmee, M. "Possible synergistic effect between high lactate and insufficient intake of peptides caused biomass reduction during high-cell starter culture production." Beneficial Microbes 1, no. 2 (June 1, 2010): 175–82. http://dx.doi.org/10.3920/bm2009.0039.

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Lactic starter culture production is normally subjected to the end-product inhibition on growth, which limits the biomass produced per production batch. Removal of lactate ions during the biomass production has improved the biomass production. It allows for the use of higher sugar concentration so that high biomass concentration can be obtained. Lactate removal by ion exchange resin during Lactococcus lactis NZ133 cultivation was applied as a strategy for enhancing the production of lactic starter culture biomass. At high lactose concentrations of 180 g/l, the unexpected reduction in the biomass was evident regardless of the remaining sugar in the fermentation broth. The amount of protein and proteins/polypeptides pattern profile during cultivation were investigated as protein availability was suspected to be the potential cause of biomass reduction during high cell cultivation applying the ion exchange technique. Reduction in biomass concentration, after its maximum of 26 g/l, was observed after the protein concentration was unchanged while the remaining lactose continued to be utilised. A sharp decrease in protein concentration following the addition of resin corresponded to the disappearance of the smear band of protein sized 6,512-26,625 Da when more resin was added to remove lactate. The smear band remained throughout the conventional batch cultivation period. Based on the results, insufficient supply of peptides caused by the loss through adsorption onto ion exchange resin which occurred at high lactate level was postulated as the most probable cause of the biomass reduction. The result also indicated an inefficient use of supplemented protein sources supplied in correspond to the increase in lactose concentration due to the presence of appreciable amounts of residual protein at the end of cultivation process.
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Vanags, J., L. Kunga, K. Dubencovs, V. Galvanauskas, and O. Grīgs. "Influence of Light Intensity and Temperature on Cultivation of Microalgae Desmodesmus Communis in Flasks and Laboratory-Scale Stirred Tank Photobioreactor." Latvian Journal of Physics and Technical Sciences 52, no. 2 (April 1, 2015): 59–70. http://dx.doi.org/10.1515/lpts-2015-0012.

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Abstract Optimization of the microalgae cultivation process and of the bioprocess in general traditionally starts with cultivation experiments in flasks. Then the scale-up follows, when the process from flasks is transferred into a laboratory-scale bioreactor, in which further experiments are performed before developing the process in a pilot-scale reactor. This research was done in order to scale-up the process from a 0.4 1 shake flask to a 4.0 1 laboratory-scale stirred-tank photobioreactor for the cultivation of Desmodesmus (D.) communis microalgae. First, the effect of variation in temperature (21-29 ºC) and in light intensity (200-600 μmol m-2s-1) was studied in the shake-flask experiments. It was shown that the best results (the maximum biomass concentration of 2.72 g 1-1 with a specific growth rate of 0.65 g g-1d-1) can be achieved at the cultivation temperature and light intensity being 25 °C and 300 μmol m2s-1, respectively. At the same time, D. communis cultivation under the same conditions in stirred-tank photobioreactor resulted in average volumetric productivities of biomass due to the light limitation even when the light intensity was increased during the experiment (the maximum biomass productivity 0.25 g 1-1d-1; the maximum biomass concentration 1.78 g 1-1).
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Campos, C. M. M., and G. K. Anderson. "The Effect of the Liquid Upflow Velocity and the Substrate Concentration on the Start-Up and Steady-State Periods of Lab-Scale UASB Reactors." Water Science and Technology 25, no. 7 (April 1, 1992): 41–50. http://dx.doi.org/10.2166/wst.1992.0137.

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This research was carried out in order to study the effect of the upflow liquid velocity (ULV), the COD concentration, the volumetric loading rate (VLR) and the hydraulic loading rate (HLR) on the start-up and steady-state performance of UASB reactors treating soluble substrate with 3,000 mgCOD/l at mesophyllic temperature (35°C). Three lab-reactors (UASB) were constructed with different liquid volumes, and also with different cross-sectional areas. The three UASB reactors were started up with the same biological loading rate (BLR), but the start-up procedures were based on different criteria. After the start-up had been carried out successfully with the formation of the granules, all the three UASB reactors were operated under steady-state conditions for up to eight different HRT(s), namely: 16, 14, 12, 10, 8, 6, 4 and 2 hours. For each of the three reactors the kinetic parameters were determined using the Monod model. Analysis of the influence of the operational factors and reactor design on the kinetic coefficients is described. The results indicate that the liquid upflow velocity has considerable effect on the sludge in this type of system, thereby acting as a selective process in the cultivation of the biomass. Analysis of sensitivity on the kinetic parameters indicated which was the most sensitive kinetic parameter for these reactors.
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Bayraktar, V. N. "COMPARATIVE ASSESSMENT OF THE LABORATORY SELECTED AND ACTIVE DRIED SACCHAROMYCES CEREVISIAE YEAST CULTURE IN BIOTECHNOLOGY OF THE BRANDY PRODUCTION." Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University 5, no. 01 (January 30, 2015): 48. http://dx.doi.org/10.15421/2015003.

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<p>Samples from different industrial grape cultivars were collected during the vintage season from the vineyard of the winery (the «Shabo» winery Company, located in the Odesa region, Ukraine). The following industrial cultivars of grapes were selected for the research: Chardonnay, Cabernet Sauvignon, Merlot, Sauvignon, Riesling Rhenish, Aligote, Rkatsiteli, Bastardo, Traminer, Telti Kuruk, Grinosh.</p> <p>The grape cultivars were cultivated on the sandy soils in the district located between the Black Sea and the Dnestrovsky estuary. Grape must derived from different grape cultivars was placed into sterile glass flasks to half of the 450ml flask volume. Each flask was carefully closed with a rubber stopper with an injection needle in it. During the fermentation process, it was necessary to remove carbon dioxide, which was present as a result of active anaerobic fermentation processes in the grape must. At the end of grape must fermentation, pure yeast cultures were isolated using traditional microbiological methods by consistent inoculation of a sample into a Petri dish with a few modifications of nutrient selective agar for yeast isolation and cultivation. Primary yeast isolation was carried out using Inhibitory Mold Agar medium (Becton Dickinson Company, USA).</p> <p>The yeast culture morphological properties were analyzed after the primary yeast culture isolation. Yeasts were identified by polymerase chain reaction (PCR) using universal yeast primers. After yeast culture identification, the next step in yeast cultivation was carried out on Wort Agar medium (Becton Dickinson Company, USA). Each isolated, and identified yeast culture was deposited in the Genebank of Japan, MAFF culture Collection, Tsukuba, Ibaraki, Japan and (NCYC) - Yeast Culture Collection (National Collection of Yeast Cultures, Institute of Food Research, Norwich, United Kingdom). Each yeast culture was tested for technological characteristics such as growth resistance to high temperature (+42°C) and low temperature (+6°C), growth at low pH 2.6–3.0 (acid resistance), growth in the presence of 5, 10, and 15% ethanol (ethanol resistance), and growth in the presence of high concentration potassium bisulfite (bisulfite resistance). Hydrosulfide synthesis (H<sub>2</sub>S gassing production) was studied in addition.</p> <p>Parameters of cellular metabolism in yeast suspension, such as concentration of nitrogen, protein, triglicerides, enzymatic activity and total sugar (which include glucose, fructose, and galactose) were determined. Macro- and micro-element concentrations in fermented grape must, which contained pure yeast culture was determined and included: potassium, sodium, calcium, phosphorus, magnesium, iron, chlorides. In addition to identifying parameters of macro- and micro- element concentration in grape must during and following fermentation based on a principle of photometric analysis, carried out using a biochemical analyser Respons-920 (DiaSys Diagnostic Systems GmbH, Germany).</p> <p>Laboratory selected <em>Saccharomyces cerevisiae </em>wine yeast showed high enzymatic activity with short lag phase. Since of fermentation started on third day concentration of Triglicerides, Protein (total), Potassium and Sodium increased and then level of Protein (total) on the 5th day of fermentation twice decreased. Trigliceride concentration on the 5th day of fermentation continued to increase. Concentration of Iron on the 5th day of fermentation increase in geometrical progression, concentration increase in 4-5 times. Contrary Chloride concentration on the 5th day of fermentation decreased in 3-4 times. Enzymatic activity on 3rd day of fermentation maximal for Lactate Dehydrogenase, Alanine aminotransferase, Aspartate aminotransferase, Phosphatase. Since of 5th day of fermentation Enzymatic activity for Lactate Dehydrogenase, Alanine aminotransferase, Aspartate aminotransferase 3-4 times. Especially level of Phosphatase activity very decreased in 6-7 times. Comparative assessment between our Laboratory selected <em>Saccharomyces cerevisiae</em> yeast culture and Dry active commercial <em>Saccharomyces cerevisiae</em> yeast culture did not showed any difference in enzymatic activity. Both groups showed high enzymatic activity on the third day from the start of fermentation and decreasing on the fifth day since of fermentation started.</p> <p><em> Key words: wine yeast, enzymatic activity, cellular metabolism</em><em>, </em><em>Saccharomyces cerevisiae</em><em>.</em><em></em></p>
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Zhang, Lin-Lin, Jie Li, Yi-Lin Wang, Song Liu, Zhi-Peng Wang, and Xin-Jun Yu. "Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9." Biomolecules 9, no. 9 (September 2, 2019): 439. http://dx.doi.org/10.3390/biom9090439.

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Tannins biodegradation by a microorganism is one of the most efficient ways to produce bioproducts of high value. However, the mechanism of tannins biodegradation by yeast has been little explored. In this study, Aureobasidium melanogenum T9 isolated from red wine starter showed the ability for tannins degradation and had its highest biomass when the initial tannic acid concentration was 20 g/L. Furthermore, the genes involved in the tannin degradation process were analyzed. Genes tan A, tan B and tan C encoding three different tannases respectively were identified in the A. melanogenum T9. Among these genes, tan A and tan B can be induced by tannin acid simultaneously at both gene transcription and protein expression levels. Our assay result showed that the deletion of tanA and tanB resulted in tannase activity decline with 51.3 ± 4.1 and 64.1 ± 1.9 U/mL, respectively, which is much lower than that of A. melanogenum T9 with 91.3 ± 5.8 U/mL. In addition, another gene coding gallic acid decarboxylase (gad) was knocked out to better clarify its function. Mutant Δgad completely lost gallic acid decarboxylase activity and no pyrogallic acid was seen during the entire cultivation process, confirming that there was a sole gene encoding decarboxylase in the A. melanogenum T9. These results demonstrated that tanA, tanB and gad were crucial for tannin degradation and provided new insights for the mechanism of tannins biodegradation by yeast. This finding showed that A. melanogenum has potential in the production of tannase and metabolites, such as gall acid and pyrogallol.
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Hrnčiřík, Pavel, Tomáš Moucha, Jan Mareš, Jan Náhlík, and Dagmar Janáčová. "Software Sensors for Biomass Concentration Estimation in Filamentous Microorganism Cultivation Process." Chemical & biochemical engineering quarterly 33, no. 1 (2019): 141–51. http://dx.doi.org/10.15255/cabeq.2018.1387.

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In this study, the potential of two software sensors for on-line estimation of biomass concentration during cultivation of filamentous microorganisms is examined. The first sensor is based on common bioreactor off-gas analyses, and uses the assumption of the biomass concentration linear dependence on the square root of cumulative O2 consumption. Parameters of the semi-empirical data-driven software sensor based on off-gas analysis were calculated from experimental cultivation data using linear regression. The second sensor is based on biocalorimetry, i.e., the on-line calculation of metabolic heat flux from general enthalpy balance of the bioreactor. The software sensor based on biocalorimetry thus essentially represents a model-driven approach, making use of a fundamental process model based on the enthalpy balance around the bioreactor. This approach has been combined with the experimental identification of the specific biomass heat production, which represents the main process-specific parameter of the software sensor based on biocalorimetry. For this sensor, the accuracy requirements on the process variable on-line measurements were also analysed. The experimental data from the pilot-scale antibiotics Nystatin production by a bacterium Streptomyces noursei were used to calculate the specific bioprocess heat production value using linear regression. The achieved results enabled us to propose a new on-line indicator calculated as the ratio of the outputs of both sensors, which can serve as a timely warning of the risk of undesired nutritional conditions of a culture characterized as underfeeding.
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Papadelli, Marina, Georgia Zoumpopoulou, Marina Georgalaki, Rania Anastasiou, Eugenia Manolopoulou, Ioanna Lytra, Kostas Papadimitriou, and Effie Tsakalidou. "Evaluation of Two Lactic Acid Bacteria Starter Cultures for the Fermentation of Natural Black Table Olives (Olea europaea L cv Kalamon)." Polish Journal of Microbiology 64, no. 3 (September 18, 2015): 265–71. http://dx.doi.org/10.5604/01.3001.0009.2121.

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The production of Greek-style natural black table olives remains an empirical process relying on spontaneous fermentation despite its economic significance. For this reason producers often resort to increased NaCl concentration of the brine to secure quality of the product. In this study we employ two lactic acid bacteria Leuconostoc mesenteroides subsp. mesenteroides Lm139 and Lactobacillus pentosus DSM 16366 as starters in separate laboratory low salinity fermentations of “Kalamon” cultivar olives, processed according to the Greek-style method. L. mesenteroides subsp. mesenteroides Lm139 was previously isolated from Kalamon olives laboratory spontaneous fermentations, while L. pentosus DSM 16366 was isolated from fermenting green olives prepared according to the Spanish-style method. Spontaneous olives fermentation was also performed as a control. Microbiological and physicochemical analyses of the brines revealed that the use of the starters had a significant effect on the olives fermentation, leading to a faster acidification due to the more efficient consumption of soluble sugars in the brines. The final pH value reached by each starter culture used indicates a successful lactic fermentation. The production of lactic acid by the starters and the concomitant drop of the pH value proved to inhibit enterobacteria in a shorter period of time compared to the spontaneous fermentation. Concluding, the use of either of the two lactic acid bacteria as starters in Greek-style Kalamon olives fermentation could lead to a more controllable fermentation at lower salinities. The resulting product could be of higher quality with extended shelf-life while being at the same time safer for the consumer.
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Ho, Quyen Bao Thuy, and Akira Suzuki. "TECHNOLOGY OF MUSHROOM CULTIVATION." Vietnam Journal of Science and Technology 57, no. 3 (June 4, 2019): 265. http://dx.doi.org/10.15625/2525-2518/57/3/12954.

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Mushroom cultivation has been developed by two ways, i. e., one based on cultivation by wood-log and another based on cultivation by non-wood-log. Non-wood-log cultivation would be categorized into two aspects. Mushroom cultivation under non-aseptic condition, i. e., one characterized by the processes of fermentation (composting) and casing, and another characterized by using sterilized media followed by cultivation process associated with strict control of environmental factors such as light, temperature, humidity, and CO2 concentration.
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Ly, Sokny, F. Bajoul Kakahi, Hasika Mith, Chanvorleak Phat, Barbara Fifani, Tierry Kenne, Marie-Laure Fauconnier, and Frank Delvigne. "Engineering Synthetic Microbial Communities through a Selective Biofilm Cultivation Device for the Production of Fermented Beverages." Microorganisms 7, no. 7 (July 20, 2019): 206. http://dx.doi.org/10.3390/microorganisms7070206.

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Production of Cambodian rice wine involves complex microbial consortia. Indeed, previous studies focused on traditional microbial starters used for this product revealed that three microbial strains with complementary metabolic activities are required for an effective fermentation, i.e., filamentous fungi (Rhizopus oryzae), yeast (Saccharomyces cerevisiae), and lactic acid bacteria (Lactobacillus plantarum). Modulating the ratio between these three key players led to significant differences, not only in terms of ethanol and organic acid production, but also on the profile of volatile compounds, in comparison with natural communities. However, we observed that using an equal ratio of spores/cells of the three microbial strains during inoculation led to flavor profile and ethanol yield close to that obtained through the use of natural communities. Compartmentalization of metabolic tasks through the use of a biofilm cultivation device allows further improvement of the whole fermentation process, notably by increasing the amount of key components of the aroma profile of the fermented beverage (i.e., mainly phenylethyl alcohol, isobutyl alcohol, isoamyl alcohol, and 2-methyl-butanol) and reducing the amount of off-flavor compounds. This study is a step forward in our understanding of interkingdom microbial interactions with strong application potential in food biotechnology.
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Patyna, Agnieszka, Małgorzata Płaczek, and Stanisław Witczak. "Study of Chlorella vulgaris sedimentation process." MATEC Web of Conferences 240 (2018): 05023. http://dx.doi.org/10.1051/matecconf/201824005023.

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The paper reports the results of Chlorella vulgaris sedimentation process including description of cultivation condition of microalgal biomass. The process of algae cultivation was carried out in photobioreactor comprising systems of carbon dioxide supply, mixing and artificial LED illumination. The growth of microalgae was determined alternatively in three ways by measuring the amount of dry mass over time, counting the cells and measurement of optical density by use of a spectrophotometer. Algae biomass with different concentration was subjected to the separation process by gravity. This led to the determination of the characteristic of sedimentation process for different concentrations and cell sizes. The experimental results indicate that sedimentation process offers a tool with a potential application for microalgae harvesting.
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Dissertations / Theses on the topic "Process cultivation concentration starters"

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Кічура, Марія Андріївна. "Технологія виробництва бактеріального концентрату закваски для кисломолочних продуктів. Дільниця виробничого біосинтезу." Bachelor's thesis, КПІ ім. Ігоря Сікорського, 2020. https://ela.kpi.ua/handle/123456789/41082.

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Дипломний проект містить 108 сторінок, 10 ілюстрацій, 3 таблиці, 2 креслиники та 109 бібліографічних найменувань за переліком посилань. Метою даної роботи є проектування виробництва бактеріальної закваски для виготовлення кисломолочних продуктів дієтичного харчування,зокрема йогурту. Робота присвячена збільшенню обсягів виробництва вітчизняних заквасок, що дасть можливість збільшення асортименту та здешевлення молочнокислих продуктів. Обгрунтовано і запропоновано в якості продуцента бактеріальної закваски використовувати штами гомоферментативних термофільних молочнокислих бактерій Lactobacillus delbrueckii subsp. Вulgaricus та Streptococcus salivarius subsp. Thermophilus, отримані у результаті селекції з використанням природнього добору , мають пробітичні властивості і є високотехнологічними для виготовлення кисломолочної пробдукції. Обрано ефективне, економічно вигідне та надійне обладнання для виробничого культивування закваски. Розраховано та обрано апарат для виробничого культивування. Наведено технологічний, конструктивний та гідравлічний розрахунки обраного ферментеру. В роботі обґрунтовані та подані технологічна та апаратурна схеми виробництва.
The diploma project contains 108pages, 10 illustrations, 3 tables, 2 drawings and 109 bibliographic titles according to the list of references. The purpose of this work is to design the production of bacterial leaven for the manufacture of fermented milk products for dietary nutrition. The work is devoted to increasing the production of domestic leavens, which will increase the range and reduce the cost of lactic acid products. It is substantiated and proposed to use strains of homofermentative thermophilic lactic acid bacteria Lactobacillus delbrueckii subsp as a producer of bacterial yeast. Vulgaricus and Streptococcus salivarius subsp. Thermophilus, obtained by selection using natural selection, have probiotic properties and are high-tech for the manufacture of fermented milk production. Efficient, cost-effective and reliable equipment for production cultivation of sourdough has been selected. The device for industrial cultivation is calculated and selected. Technological, constructive and hydraulic calculations of the selected fermenter are given. The technological and hardware schemes of production are substantiated and presented in the work
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Book chapters on the topic "Process cultivation concentration starters"

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Kumar Gupta, Puneet, and Jyotheeswara Reddy Edula. "Strategies for Enhancing Product Yield: Design of Experiments (DOE) for Escherichia coli Cultivation." In Fermentation - Processes, Benefits and Risks [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.99288.

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E. coli is considered one of the best model organism for biopharmaceutical production by fermentation. Its utility in process development is employed to develop various vaccines, metabolites, biofuels, antibiotics and synthetic molecules in large amounts based on the amount of yield in shake flasks, bioreactors utilised by batch, fed-batch and continuous mode. Production of the desired molecule is facilitated in the bioreactor by employing strategies to increase biomass and optimised yield. The fermentation is a controlled process utilising media buffers, micronutrients and macronutrients, which is not available in a shake flask. To maximise the production temperature, dissolved oxygen (aerobic), dissolved nitrogen (anaerobic), inducer concentration, feed or supplementation of nutrients is the key to achieving exponential growth rate and biomass. Design of experiments (DOE) is critical for attaining maximum gain, in cost-effective manner. DOE comprises of several strategies likewise Plakett-Burman., Box–Behnken, Artificial Neural Network, combination of these strategies leads to reduction of cost of production by 2–8 times depending on molecules to be produced. Further minimising downstream process for quickly isolation, purification and enrichment of the final product.
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Roeva, Olympia, Tsonyo Slavov, and Stefka Fidanova. "Population-Based vs. Single Point Search Meta-Heuristics for a PID Controller Tuning." In Handbook of Research on Novel Soft Computing Intelligent Algorithms, 200–233. IGI Global, 2014. http://dx.doi.org/10.4018/978-1-4666-4450-2.ch007.

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This chapter presents a comparison of population-based (e.g. Genetic Algorithms (GA), Firefly Algorithm (FA), and Ant Colony Optimization (ACO))and single point search meta-heuristic methods (e.g. Simulated Annealing (SA), Threshold Accepting (TA), and Tabu Search (TS)) applied to an optimal tuning of a universal digital proportional-integral-derivative (PID) controller. The PID controllers control feed rate and maintain glucose concentration at the desired set point for an E. coli MC4110 fed-batch cultivation process. The model of the cultivation process is represented by dynamic non-linear mass balance equations for biomass and substrate. In the control the design measurement, process noise, and time delay of the glucose measurement system were taken into account. To achieve good closed-loop system the constants (Kp, Ti, Td, b, c and N) were tuned in the PID controller algorithm so the controller can provide control action designed for the specific process requirements resulting in an optimal set of PID controller settings. For a time the controllers set and maintained the control variable at the desired set point during the E. coli MC4110 fed-batch cultivation process. Average, best, and worst objective function values and PID controller's parameters were used as criteria to compare the performance of the considered meta-heuristic algorithms. This indicates that the population-based meta-heuristics performs better than the single point search methods. GA and ACO show better performance than FA. It also indicates that TS results are comparable to those of FA. The results show that SA and TA algorithms failed to solve the PID controller tuning problem.
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Czekaj-Zastawny, Agnieszka, and Robert Kenig. "Igołomska terasa Wisły w VI tysiącleciu p.n.e. – czas pierwszych rolników Igołomia terrace of the Vistula River in the 6th millennium BC – time of the first farmers." In Kartki z dziejów igołomskiego powiśla, 73–91. Wydawnictwo i Pracownia Archeologiczna PROFIL-ARCHEO, 2020. http://dx.doi.org/10.33547/igolomia2020.05.

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The introduction of an agriculture-economy based on the cultivation of plants and animal husbandry – known as Neolithization – was a breakthrough in human history. The lifestyle that had been led since the dawn of humanity, seasonal migration to search for sources of food, changed relatively abruptly. In Central Europe, including Poland, this process was associated with the appearance of the first farmers known under the archaeological term of the Linear Pottery culture (LBK). Intensive spreading and concentration of settlement occurred at the end of the first phase, reaching peak intensity in the middle phase. Most large, permanently inhabited settlements with longhouses are dated to this period, similar to in other centres in the Odra and Vistula basins. About half of the 1,600 sites associated with LBK in Poland are located in south-eastern Poland. Due to favourable environmental conditions, the Vistula left-bank terrace in the area of what is today Igołomia-Wawrzeńczyce commune, in Kraków district has been intensively populated since the beginning of the Neolithic.
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Czekaj-Zastawny, Agnieszka, and Robert Kenig. "Igołomska terasa Wisły w VI tysiącleciu p.n.e. – czas pierwszych rolników / Igołomia terrace of the Vistula River in the 6th millennium BC – time of the first farmers." In Kartki z dziejów igołomskiego powiśla, 71–88. 2nd ed. Wydawnictwo i Pracownia Archeologiczna Profil-Archeo, 2021. http://dx.doi.org/10.33547/igolomia2021.05.

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The introduction of an agriculture-economy based on the cultivation of plants and animal husbandry – known as Neolithization – was a breakthrough in human history. The lifestyle that had been led since the dawn of humanity, seasonal migration to search for sources of food, changed relatively abruptly. In Central Europe, including Poland, this process was associated with the appearance of the first farmers known under the archaeological term of the Linear Pottery culture (LBK). Intensive spreading and concentration of settlement occurred at the end of the first phase, reaching peak intensity in the middle phase. Most large, permanently inhabited settlements with longhouses are dated to this period, similar to in other centres in the Odra and Vistula basins. About half of the 1,600 sites associated with LBK in Poland are located in south-eastern Poland. Due to favourable environmental conditions, the Vistula left-bank terrace in the area of what is today Igołomia-Wawrzeńczyce commune, in Kraków district has been intensively populated since the beginning of the Neolithic.
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Chidi, Nosiri, Anyanwu Chukwuma, and Nwaogwugwu Joel. "Impact of Emerging Agricultural Contaminants on Global Warming." In Emerging Contaminants. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.94170.

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There are many definitions of emerging contaminants (ECs). They are not usually new chemicals and could be substances that have stayed long in the environment with their presence and importance being recognized now. They may be chemicals or microorganisms which are not usually monitored in the environment but with known or suspected potential to cause ecological damage or adverse human effects. Some natural products and those transformed through biochemical processes from synthetic chemicals may be formed in the environment as ECs. Emerging Agricultural Contaminants are released to the environment or enter indirectly to the soil during the application of manure, fertilizers, biosolids or other solid waste materials. Once they enter the soil, they may be transported by leaching, runoff and drainage processes to water bodies. The extent of the transport is dependent on the persistence of the EC and on how it interacts with soil and air. These ECs contribute to global warming through the emissions of Greenhouse gases. The largest source of GHG emission from Agriculture is Nitrous oxide (N2O) and it accounts for 38% of the total global emission through the process of nitrification and denitrification, anthropogenic activities (use of nitrogen fertilizer, production of nitrogen-fixing crops and forages, retention of crop residues, application of managed livestock manure) which are either through direct additions and/or through indirect additions (atmospheric deposition of applied nitrogen). The natural digestive processes in ruminants otherwise known as enteric fermentation account for the key source of methane production under livestock production hence the second largest source of total agricultural emission with 34% global share and rice cultivation being the third with 11%. The three important greenhouse gases (Methane, Carbon dioxide and Nitrous oxide) are not harmful in naturally occurring quantities for their atmospheric presence helps in sustaining life on the planet when they trap heat energy near the surface of the earth. Concentration of greenhouse gases from both the natural and human factors have been increasing and contributing to Global Warming and Climate Change. Increase in greenhouse gases may cause tremendous changes to our civilization positively or negatively but the total impact is uncertain. Climate change comes as a result of a warming planet which can affect the weather adversely in many ways. So, as climate changes, extreme weather activities release severe threats on human society. Indicators of global warming include sea surface temperature, temperature over land, snow cover on hills, temperature over land and humidity. It is expected that climate change may cause more floods, storms, droughts, heatwaves and other extreme weathers activities. IPCC estimated that temp may rise from 2 to 6°C within 2021. Mitigation of greenhouse effect could be achieved through Biochemical methods on enteric fermentation, development of good environmental policies even Methanotrophs also aid in recycling the atmospheric Methane.
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"subsistence production (where in the colonial period mainly extra-economic factors such as forced cultivation or forced labour caused the integration of the peasantry in the market exchange). Socialist development was there-fore strongly identified with modernising through the rapid expansion of the state sector, that is, nationalisation and mechanisation on an ever-increasing scale. The peasantry would be gradually absorbed within this expanding sector, and hence, at first, the role of the peasantry was seen as essentially passive with its transformation mainly centring on social aspects. As such, the policy of communal villages became virtually a habitational concept (and was in actual fact the responsibility of the national directorate of housing): a question of social infrastructures (water supplies, schools, etc.) within a concept of communal life without concerning production and its transformation. This view conflicted heavily with the objective conditions in the rural areas characterised by a deep involvement of the peasantry in market relationships and their dependence on it either as suppliers of labour power or as cash crop producers. This contradiction became more obvious, when the balance of payments became a real constraint (in 1979) and, hence, the question of financing accumulation cropped up more strongly in practice. The peasantry as suppliers of cash crops, of food and of labour power to the state sectors occupied a crucial position in production and accumulation. However, the crucial question then becomes whether the peasantry only performs the role of supplying part of the accumulation fund or whether the peasantry itself is part and parcel of the process of transformation and hence that accumulation embraces as an integral part the transformation of peasant agriculture into more socialised forms of production. In other words, it poses the question whether the strategy is based on a primitive socialist accumulation on the basis of the peasantry (transferring the agrarian surplus to the develop-ment of the state sector), or whether accumulation includes the transformation of peasant agriculture. Clearly, the way this question is posed in practice will influence heavily the nature of the organisation of the exchange between the state sector and the peasantry. The proposition that the state sector can develop under its own steam (with or without the aid of external borrowing) cannot bypass this crucial question since, on the one hand, a considerable part of foreign exchange earnings and of the food supply to the towns depended on peasant production and, on the other, the very conditions of productivity and profitability in the agrarian state sector depended heavily on the organic link that existed.between labour supply and family agriculture. The monetary disequilibrium originating from the state sector has a severe impact on the organisation of the exchange between the state sector and the peasantry. First, the imbalance between the demand for and the supply of consumer commodities affected rural areas differently from urban areas. The reason was that in urban areas the rationing system guaranteed to each family a minimum quantity of basic consumer necessities at official prices. In the rural areas the principal form of rationing remained the queue! Hence, forced savings were distributed differently over urban and rural areas. Furthermore, the concentration of resources on the state sector also implied that the peasants'." In The Agrarian Question in Socialist Transitions, 205. Routledge, 2013. http://dx.doi.org/10.4324/9780203043493-29.

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"levels which normally oscillated between 80,000 and 100,000 per year, and which in 1975 had soared up to 118,000 workers, were sharply reduced to 40,000 thereafter [First, 1982]. This mainly affected the southern part of Mozambique by creating massive rural unemployment. The towns had no capacity to absorb this surplus labour since employment was drastically re-duced in the towns as well. The latter process was due to the fall in employ-ment in domestic work (servants) and in the tourist sector (restaurants, hotels, bars, etc.). The exodus of Portuguese settlers and the virtual standstill of tourism (which catered for South Africans and Rhodesians) had amplified the problem of structural employment in the towns. The rural unemployed could not merely fall back on family agriculture since this was heavily dependent on cash income from wage work. Oxen and ploughs, farm implements, water reserves, etc. were normally paid for with wages from mine labour or other wage work. Furthermore, due to this cash inflow from wage income, a more interactive type of division of labour developed within the rural areas of southern Mozambique. Hence, peasants without oxen and plough would rent the services of peasants who did, and pay for it out of wage income. Brick-makers, carpenters, house-builders, tailors, mechanics were to be found among the middle peasantry who relied on these activities (usually acquired through mine labour) to supplement their income from farming. In a similar fashion, local transport and petty com-merce were sidelines of middle peasants stabilised by the influx of wage income. The reduction in mine labour employment deeply affected the viability of this internal division of labour within the rural economy. Finally, the impact of the reduction in mine labour was not evenly spread among the peasantry, since only those who held valid work certificates from the recruitment agency could continue to go to the mines. Other peasants were cut off altogether. This introduced a sharp element of differentiation within the rural econonmy. Those who could continued to go to the mines not only had cash income but also a guaranteed access to commodities (including means of production), while within Mozambique shortages were rapidly turning into a goods famine. However, rural unemployment was not merely a phenomena of the south. In central Mozambique, wage work to Rhodesia dropped sharply with the closure of the border between Mozambique and Rhodesia since 1976, and as a result of the war situation which developed thereafter. As stated in above, the concentration of resources on the state sector further weakened the basis of family agriculture at a time when a considerable part of its cash income through wage labour was cut off. While the colonial situ-ation was characterised by persistent labour shortages within the rural economy and continued state intervention to keep labour cheap (through the imposition of forced labour and forced cultivation of crops as well as by fragmentation of labour markets to avoid competition for labour to drive up the wage levels), the post-independence situation became characterised by rural unemployment and an intensified flow of people from the rural areas to the towns in search of wage work. The priority accorded to investments led to the slow expansion in the supply of consumer goods and in 1981 it actually fell by eight per cent: six per cent." In The Agrarian Question in Socialist Transitions, 197–204. Routledge, 2013. http://dx.doi.org/10.4324/9780203043493-28.

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Conference papers on the topic "Process cultivation concentration starters"

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Ma, Jian, and Oliver Hemmers. "Thermo-Economic Analysis of Microalgae Co-Firing Process for Fossil Fuel-Fired Power Plants." In ASME 2010 4th International Conference on Energy Sustainability. ASMEDC, 2010. http://dx.doi.org/10.1115/es2010-90186.

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A thermoeconomic analysis of microalgae co-firing process for fossil fuel-fired power plants is studied. A process with closed photobioreactor and artificial illumination is evaluated for microalgae cultivation, due to its simplicity with less influence from climate variations. The results from this process would contribute to further estimation of process performance and investment. The concept of co-firing (coal-microalgae or natural gas-microalgae) includes the utilization of CO2 from power plant for microalgal biomass culture and oxy-combustion of using oxygen generated by biomass to enhance the combustion efficiency. As it reduces CO2 emission by recycling it and uses less fossil fuel, there are concomitant benefits of reduced GHG emissions. The by-products (oxygen) of microalgal biomass can be mixed with air or recycled flue gas prior to combustion, which will have the benefits of lower nitrogen oxide concentration in flue gas, higher efficiency of combustion, and not too high temperature (avoided by available construction materials) resulting from coal combustion in pure oxygen. Two case studies show that there are average savings about $0.386 million/MW/yr and $0.323 million/MW/yr for coal-fired and natural gas-fired power plants, respectively. These costs saving are economically attractive and demonstrate the promise of microalgae technology for reducing greenhouse gas (GHG) emission.
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Sharifullina, D. T., R. N. Nizamov, R. N. Nizamov, I. R. Yunusov, and G. I. Rakhmatullina. "STUDYING THE POSSIBILITY OF JOINT CULTIVATION OF B.BIFIDUM AND E.COLI ON ADAPTED NUTRIENT MEDIA." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS Volume 2. DSTU-Print, 2020. http://dx.doi.org/10.23947/interagro.2020.2.423-426.

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Microbial substances introduced into the body of animals increase radio stability and reduce the mortality rate. The greatest significance can be obtained by using vaccines based on bacteria of the intestinal-typhoid group, which in the process of life produce antibacterial substances, enzymes, antigens, entero-and exotoxins, and cytokines with radioprotective properties. The tests revealed a complex mechanism of interaction between bifidobacteria and Escherichia in their joint cultivation. The biomass accumulation of E.coli strain «PL-6» and B.bifidum 1 during co-cultivation depended on the ratio of live bacteria E.coli strain «PL-6» and B.bifidum 1. Microcopy of smears made on days 1-4 from monocultures showed that the grown microbes in morphology corresponded to these cultures. The concentration of microorganisms, determined by tenfold dilution by the above method, was 1x109 CFU/ml - E.coli and 1x107 CFU/ml B.bifidum, with a sowing dose of each type of microbe 1x108 CFU/ml. Microcopy of smears made from a mixture of cultures showed that a dilution of 0,9:1,1-1,0:1,0 is most optimal for co-growing bifidum and Escherichia coli, since with a relatively equal number of monocultures on the 1st day Escherichiae multiply intensely, splitting the components of the Blaurock medium and inhibiting the growth of bifidum, but from the 3rd day B.bifidum begins to prevail, splitting E.coli and assimilating substances cleaved by E.coli.
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Berberog˘lu, Halil, and Laurent Pilon. "Symbiotic Cultures for Increasing the Solar Energy Conversion Efficiency of Outdoor Photobioreactors." In ASME 2009 Heat Transfer Summer Conference collocated with the InterPACK09 and 3rd Energy Sustainability Conferences. ASMEDC, 2009. http://dx.doi.org/10.1115/ht2009-88249.

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A numerical study is presented aiming to maximize the solar to hydrogen energy conversion efficiency of a symbiotic culture containing microorganisms with different absorption characteristics. The green algae Chlamydomonas reinhardtii CC125 and the purple non-sulfur bacteria Rhodobacter sphearoides ATCC 49419 are chosen for illustration purposes. The previously measured radiation characteristics of each microorganism are used as input parameters in the radiative transport equation for calculating the local spectral incident radiation within a flat panel photobioreactor. The specific hydrogen production rate for each microorganism as a function of the available incident radiation is recovered from data reported in the literature. The overall solar to hydrogen energy conversion efficiency of symbiotic cultures of varying microorganism concentrations have been computed for photobioreactor thicknesses from 1 to 10 cm. The results show that for a given photobioreactor thickness a saturation microorganism concentration exists above which the solar energy conversion efficiency does not increase. The maximum solar energy conversion efficiencies of solo cultures of C. reinhardtii and R. spaheroides at their respective saturation concentrations are 0.06 and 0.055%, respectively. Using symbiotic cultures, a total conversion efficiency of about 0.075% is achieved within the parameter range explored. It has been shown that the choice of microorganism concentrations for maximum solar energy conversion efficiency is non-trivial and requires careful radiation transfer analysis coupled with H2 production kinetics taking into account the photobioreactor thickness. The presented numerical tool can be used for simulating any photobiological or photochemical process involving more than one species with different radiation characteristics provided the closure laws for the reaction kinetics are known as a function of spectral incident radiation. Examples include (i) the symbiotic cultivation of more than one microorganism for biomass or lipid production in a photobioreactor and (ii) a photochemical reactor containing a number of absorbing and scattering photocatalysts with different band gaps.
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Reports on the topic "Process cultivation concentration starters"

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Granot, David, Richard Amasino, and Avner Silber. Mutual effects of hexose phosphorylation enzymes and phosphorous on plant development. United States Department of Agriculture, January 2006. http://dx.doi.org/10.32747/2006.7587223.bard.

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Research objectives 1) Analyze the combined effects of hexose phosphorylation and P level in tomato and Arabidopsis plants 2) Analyze the combined effects of hexose phosphorylation and P level in pho1 and pho2 Arabidopsis mutants 3) Clone and analyze the PHO2 gene 4) Select Arabidopsis mutants resistant to high and low P 5) Analyze the Arabidopsis mutants and clone the corresponding genes 6) Survey wild tomato species for growth characteristics at various P levels Background to the topic Hexose phosphorylating enzymes, the first enzymes of sugar metabolism, regulate key processes in plants such as photosynthesis, growth, senescence and vascular transport. We have previously discovered that hexose phosphorylating enzymes might regulate these processes as a function of phosphorous (P) concentration, and might accelerate acquisition of P, one of the most limiting nutrients in the soil. These discoveries have opened new avenues to gain fundamental knowledge about the relationship between P, sugar phosphorylation and plant development. Since both hexose phosphorylating enzymes and P levels affect plant development, their interaction is of major importance for agriculture. Due to the acceleration of senescence caused by the combined effects of hexose phosphorylation and P concentration, traits affecting P uptake may have been lost in the course of cultivation in which fertilization with relatively high P (30 mg/L) are commonly used. We therefore intended to survey wild tomato species for high P-acquisition at low P soil levels. Genetic resources with high P-acquisition will serve not only to generate a segregating population to map the trait and clone the gene, but will also provide a means to follow the trait in classical breeding programs. This approach could potentially be applicable for other crops as well. Major conclusions, solutions, achievements Our results confirm the mutual effect of hexose phosphorylating enzymes and P level on plant development. Two major aspects of this mutual effect arose. One is related to P toxicity in which HXK seems to play a major role, and the second is related to the effect of HXK on P concentration in the plant. Using tomato plants we demonstrated that high HXK activity increased leaf P concentration, and induced P toxicity when leaf P concentration increases above a certain high level. These results further support our prediction that the desired trait of high-P acquisition might have been lost in the course of cultivation and might exist in wild species. Indeed, in a survey of wild species we identified tomato species that acquired P and performed better at low P (in the irrigation water) compared to the cultivated Lycopersicon esculentum species. The connection between hexose phosphorylation and P toxicity has also been shown with the P sensitive species VerticordiaplumosaL . in which P toxicity is manifested by accelerated senescence (Silber et al., 2003). In a previous work we uncovered the phenomenon of sugar induced cell death (SICD) in yeast cells. Subsequently we showed that SICD is dependent on the rate of hexose phosphorylation as determined by Arabidopsis thaliana hexokinase. In this study we have shown that hexokinase dependent SICD has many characteristics of programmed cell death (PCD) (Granot et al., 2003). High hexokinase activity accelerates senescence (a PCD process) of tomato plants, which is further enhanced by high P. Hence, hexokinase mediated PCD might be a general phenomena. Botrytis cinerea is a non-specific, necrotrophic pathogen that attacks many plant species, including tomato. Senescing leaves are particularly susceptible to B. cinerea infection and delaying leaf senescence might reduce this susceptibility. It has been suggested that B. cinerea’s mode of action may be based on induction of precocious senescence. Using tomato plants developed in the course of the preceding BARD grant (IS 2894-97) and characterized throughout this research (Swartzberg et al., 2006), we have shown that B. cinerea indeed induces senescence and is inhibited by autoregulated production of cytokinin (Swartzberg et al., submitted). To further determine how hexokinase mediates sugar effects we have analyzed tomato plants that express Arabidopsis HXK1 (AtHXK1) grown at different P levels in the irrigation water. We found that Arabidopsis hexokinase mediates sugar signalling in tomato plants independently of hexose phosphate (Kandel-Kfir et al., submitted). To study which hexokinase is involved in sugar sensing we searched and identified two additional HXK genes in tomato plants (Kandel-Kfir et al., 2006). Tomato plants have two different hexose phosphorylating enzymes; hexokinases (HXKs) that can phosphorylate either glucose or fructose, and fructokinases (FRKs) that specifically phosphorylate fructose. To complete the search for genes encoding hexose phosphorylating enzymes we identified a forth fructokinase gene (FRK) (German et al., 2004). The intracellular localization of the four tomato HXK and four FRK enzymes has been determined using GFP fusion analysis in tobacco protoplasts (Kandel-Kfir et al., 2006; Hilla-Weissler et al., 2006). One of the HXK isozymes and one of the FRK isozymes are located within plastids. The other three HXK isozymes are associated with the mitochondria while the other three FRK isozymes are dispersed in the cytosol. We concluded that HXK and FRK are spatially separated in plant cytoplasm and accordingly might play different metabolic and perhaps signalling roles. We have started to analyze the role of the various HXK and FRK genes in plant development. So far we found that LeFRK2 is required for xylem development (German et al., 2003). Irrigation with different P levels had no effect on the phenotype of LeFRK2 antisense plants. In the course of this research we developed a rapid method for the analysis of zygosity in transgenic plants (German et al., 2003).
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