Dissertations / Theses on the topic 'Primers'
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Hosen, Joshua Carter. "Fundamental Analysis of Wood Adhesion Primers." Thesis, Virginia Tech, 2010. http://hdl.handle.net/10919/76869.
Full textMaster of Science
Chiu, Angela Chen-Yen. "DNA Typing of HLA-B by PCR with Primer Mixes Utilizing Sequence-Specific Primers." Thesis, University of North Texas, 1997. https://digital.library.unt.edu/ark:/67531/metadc278947/.
Full textAngeli, Flora. "Micromachined substrates as primers for cartilage regeneration." Thesis, University of Strathclyde, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424244.
Full textMori, Shigeo. "Water based adhesive primers on aluminum substrates." Thesis, This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-10102009-020403/.
Full textValente, Daine Valente. "Prospecção e transferibilidade de marcadores est-ssr usados para análises filogenéticas em poa annua l." Universidade Federal do Pampa, 2016. http://dspace.unipampa.edu.br:8080/xmlui/handle/riu/361.
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Poa annua L. é a única espécie invasora de plantas com flores que obteve sucesso reprodutivo na Antártica, constituindo uma ameaça para as espécies nativas desse ecossistema. A hipótese da origem e colonização dessa gramínea nesse ambiente extremo é a de que as plantas pioneiras teriam vindo da Polônia, porém não é descartada a possibilidade de mútiplos eventos de introdução e diferentes fontes de distribuição. A disponibilidade de dados de sequências expressas (EST) tem facilitado o desenvolvimento de marcadores microssatélites (SSR) que podem ser utilizados como ferramentas para estudos populacionais em diferentes níveis, fluxo gênico, níveis de parentesco e informações sobre padrões filogeográficos. O objetivo desse trabalho foi desenvolver marcadores microssatélites a partir de sequências de regiões expressas da família Poaceae, testar o potencial de transferência em P. annua e utilizar esses marcadores para análise filogeográfica de P. annua, a fim de esclarecer a origem e colonização dessa espécie na Antártica. A prospecção de marcadores microssatélites foi desenvolvida com ferramentas de bioinformática, através de análises in sílico SSR em banco de dados EST para família Poaceae, disponíveis no Genbank (NCBI). Foram utilizados os programas CAP3 e SSRLocator para prospecção dos marcadores microssatélites. Uma pesquisa de Termos Gene Ontology (GO) foi realizada no banco de dados de sequências ESTs para avaliar associações entre locus SSR e processos biológicos, componentes celulares e função molecular de genes conhecidos, utilizando os programas Blast2GO e Revigo. O teste de transferência dos primers e análise molecular de P. annua foram conduzidos através da Reação em Cadeia da Polimerase (PCR). Foram prospectadas uma lista de 568 pares de primers, destes foram sintetizados 28 marcadores microssatélites para a transferência em P. annua. 68% dos marcadores EST-SSR tiveram potencial de transferência para esta espécie. A análise sugere que as amostras da Antártica são diferentes das amostras do Chile, Brasil, Irlanda e Argentina. Além disso, foram encontrados 613 transcritos divididos em 302 famílias gênicas. Com esta análise, foi possível desenvolver ferramentas moleculares para a análise genética com P. annua e outras espécies de gramíneas, mapear os motivos mais frequentes e funções dos genes em cada locus SSR, e sugerir que os diásporos de P. annua encontrados na Antártica podem ter vindos de fontes distintas das populações da America do Sul.
Poa annua L. is the only invasive species of flowering plants that reached reproductive success in Antarctica, posing a threat to native species of this ecosystem.The hypothesis of the origin and colonization of grass in this extreme environment is the pioneer plants would have come from Poland, but it is not ruled out event of multiple introduction and different sources of distribution. Recent increase in the availability of expressed sequence data (EST) has facilitated the development of microsatellite markers (SSR) can be used as tools for population studies at different levels, gene flow, relationship of levels and patterns phylogeographical information. The objective of this study was to develop microsatellite markers from expressed sequence regions of the Poaceae family, test the potential transfer in P. annua and use these markers for phylogeographic analysis of P. annua in order to clarify the origin and colonization of this species in Antarctica. The prospect of microsatellite markers was developed with bioinformatics tools, through an analysis in silico SSR in EST database to Poaceae family, available in Genbank (NCBI). Were used the CAP3 and SSRLocator programs for prospecting of microsatellite markers. A Search terms Gene Ontology (GO) were performed in ESTs sequences database to evaluate associations between SSR locus and biological processes, cellular components and molecular function of known genes, using the Blast2GO and Revigo programs. The transfer test of primers and molecular analysis of P. annua was conducted by Polymerase Chain Reaction (PCR). Were prospected a list of 568 primer pairs, these were synthesized 28 microsatellite markers for the transfer in P. annua. 68% of EST-SSR markers have potential transfer for this species. The analysis suggests that the samples from Antarctica are different from samples from Chile, Brazil, Argentina and Ireland. In addition, they found 613 transcripts divided into 302 genic families. With this analysis, it was possible to develop molecular tools for genetic analysis with P. annua and other grass species, mapping the most frequent motifs and functions of genes in each SSR locus, and suggest that the introduction of P. annua found in Antarctica may have come from sources other than South American populations.
Haider, Nadia. "Development and Use of Universal Primers in Plants." Thesis, University of Reading, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506069.
Full textArranz, Garcia Sara. "Primers episodis psicòtics, cànnabis, factors d'estrès, gènere i ètnia." Doctoral thesis, Universitat Rovira i Virgili, 2020. http://hdl.handle.net/10803/670353.
Full textLa esquizofrenia es una enfermedad compleja que se origina por una alteración del neurodesarrollo causada por la interacción de factores genéticos y ambientales a lo largo de las diferentes etapas de la vida. El primer episodio psicótico sucede en épocas tempranas, entre la adolescencia o los adultos jóvenes. La probabilidad de sufrir un primer episodio psicótico tiene que ver con la interacción de la vulnerabilidad genética individual y los factores ambientales. El estrés es uno de los principales factores implicados en la etiología de las psicosis dentro del modelo diátesis-estrés y por tanto tiene especial relevancia la relación de las medidas del eje hipotálamo-hipofisario-adrenal. En nuestro estudio hemos querido centrarnos también en unos de los principales riesgos ambientales evitables, que es el consumo de cannabis. Y ver su interacción con los factores estresantes como son el trauma infantil y los acontecimientos vitales estresantes en pacientes con psicosis de inicio reciente comparados con controles. Posteriormente hemos valorado el efecto de estos factores ambientales sobre las medidas del eje hipotálamo-hipofisario-adrenal. Para continuar hemos explorado las características clínicas de los pacientes de origen marroquí versus la población autóctona, siempre en una muestra de primeros episodios y observando el uso de cannabis. Y para finalizar, las diferencias de género, el consumo de cannabis y las razones para continuar el consumo. En conjunto, nuestro estudio muestra que el principal factor de riesgo para desarrollar una psicosis es el maltrato infantil (de los factores que hemos estudiado), potenciándose junto al consumo de cánnabis que incrementa el riesgo. En cambio, no hemos encontrado relación con el cortisol en los primeros episodios que consumían cannabis. Y en nuestros pacientes marroquíes que tienden más a ser hombres, a llevar más tratamiento inyectable y a consumir menos cannabis que la población autóctona. En cuanto al género, No hemos encontrado diferencias clínicas en el consumo de cánnabis, salvo mayor consumo para relajarse y mayor GAF al alta en mujeres.
The aetiology of schizophrenia is multi-factorial, consisting of interactions between genetic vulnerability and environmental risk factors. Schizophrenia is considerer as an illness of the neurodevelopment. The probability of suffering a first psychosis episode is an interaction between the genetic vulnerability and the environmental factors. According to the model of diathesis-stress, stress is one of the most important risk factors i the aetiology of schizophrenia, given relevance to obtain the measures of the hypothalamic-pituitary-adrenal axis function. In our study we want to study one of the most avoidable environmental risk, cannabis use. Cannabis is the third most common drug of dependence in the world, after tobacco and alcohol. There is now strong evidence that cannabis use is a risk factor for the development of psychosis. And determine whether the risk of psychosis depends on the severity of exposure to childhood trauma, recent life events and cannabis use and to determine whether there is an interaction and/or a cumulative effect among these environmental factors. In conclusion, our study provides evidence for a cumulative and a dose–response effect of environmental factors in the development of early psychosis. We did not find a relationship between HPA axis and first episodes. We find that our Moroccan patients tend to be male and tend to have LAI prescription more than native population. Considering gender, female show more "relax” as reason of use and a higher GAF at discharge.
Thompson, Denis. "Finding homologous genes with primers designed using evolutionary models." NCSU, 2003. http://www.lib.ncsu.edu/theses/available/etd-10232003-122816/.
Full textRodriguez, Noris Marcela. "PCR diagnosis of Leishmania using nuclear and kinetoplast primers." Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627544.
Full textCave, Nigel Graeme. "The adsorption and adhesion of long-chained organosilicon primers." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/47795.
Full textBattocchi, Dante. "The Development, Characterization and Testing of Mg-rich Primers." Diss., North Dakota State University, 2012. https://hdl.handle.net/10365/26453.
Full textLisboa, Marcio Vieira. "Estudo da união adesiva entre cimentos resinosos e ligas a base de níquel-cromo, em funçao da aplicação de primers adesivos metálicos." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/23/23137/tde-27092005-163931/.
Full textThe bond strength for resin and basic metals comes from the interaction between oxides in the metal surface and adhesive monomers in the composite resins composition. The literature shows that still are some questions about this interaction so this research intends to: verify if the presence of Be at Ni-Cr alloy plays some influence for this union, compare two resin luting cements at this function and study the influence the use of metal primers for noble metals in this mecanism of adhesion. After 2500 thermocycles, tensile bond strength tests were loaded at 160 specimens, which consisted of two metals discs with 6 mm in diameter and 2 mm thick from a Ni-Cr-Be alloy (Verabond Aalba Dental Inc., Cordelia, CA-USA) bonded togheter and two metal discs from a Ni-Cr alloy (Durabond - Odonto Comercial Importadora Ltda, São Paulo, SP) bonded togheter. Diferent brand adhesive systems were used: the resin cements, Panavia F (Kuraray Co. Ltd.) and Bistite II DC (Tokuyama Corp.) and the metal primers, Alloy Primer (Kuraray Co. Ltd.), Metaltite (Tokuyama Corp.) and Cesead Opaque Primer (Kuraray Co. Ltd.). Nonprimed specimens served as control group. The results indicated that there is statiscal diferences beetwen the two alloys (Ni-Cr-Be 24,82 ± 7,6 MPa e Ni-Cr - 22,32 ± 7,9 MPa). Panavia F had founded greater bond strength (27,49 ± 4,8 MPa) than Bistite DC (19,63 ± 8,4 MPa). The metal primers did not show any positive influence for the Panavia bond strength when we considere that the no primer group had 28,18 ± 5,6 MPa bond strength media which were equivalente to the others Panavia´s groups. However for the Bistite cement, the bond strength for Alloy primer and Metaltite were the lower medias, 12,7 ± 7,6 MPa and 15,71 ± 4,8 MPa respectivally, while the no primer or the Cesead Opaque Primer groups showed superior medias 24,47 ± 6 MPa and 25,70 ± 6,8 MPa respectivally. The outcome of this research shows that the metal primers used did not incresead the bond strength between resin cements and Ni-Cr alloys.
Lopes, Raquel de Oliveira. "Efici?ncia dos adesivos universais e primers na ades?o ? zirc?nia." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2018. http://tede2.pucrs.br/tede2/handle/tede/8121.
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Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
(Artigo Os adesivos universais s?o t?o eficientes quanto um primer para a ades?o ? zirc?nia?) O objetivo do trabalho foi comparar a resist?ncia de uni?o ? cer?mica de zirc?nia de quatro adesivos universais e um primer para zirc?nia. Setenta e cinco amostras de zirc?nia foram confeccionadas e inclu?das em resina acr?lica. A superf?cie das amostras foi polida com lixas de carbeto de sil?cio nas granula??es 600, 800 e 1.200 e jateadas com ?xido de alum?nio 50 ?m por 5 s. As amostras foram divididas aleatoriamente em cinco grupos (n=15): G1 ? Single Bond Universal (SBU); G2 ? All Bond Universal (ABU); G3 ? Peak Universal Bond (PUB); G4 ? Ambar Universal (AU) e G5 ? Z-Prime Plus (ZP). Um cone de resina composta foi constru?do, atrav?s de uma matriz, sobre o material adesivo aplicado na superf?cie das amostras. Os corpos de prova foram armazenados em ?gua destilada a 37?C por 24 h, sendo submetidos ao teste de resist?ncia de uni?o ? tra??o em m?quina de ensaio universal EMIC com velocidade de 0,5 mm/min. Os tipos de falhas foram classificados em adesiva, coesiva ou mista. Os valores de resist?ncia de uni?o foram submetidos ? an?lise de vari?ncia (ANOVA), seguido do teste de Tukey (?=0,05). M?dias de resist?ncia de uni?o (MPa) seguidas de letras distintas diferem estatisticamente entre si: G5=21,12a, G1=20,55a, G4=19,12ab, G2=14,22b, G3=8,45c. As falhas foram predominantemente mistas no G1, G4 e G5, e predominantemente adesivas no G2 e G3. Os adesivos SBU e AU obtiveram resist?ncia de uni?o compar?vel ao ZP.
(Artigo Bond to zirconia ceramic: evaluation of different primers and a universal adhesive) The aim of the study was to evaluate the effect of a universal adhesive and different primers on the bond strength to zirconia ceramic. Seventy-five zirconia ceramic samples were obtained and divided into five groups (n=15): G1 ? Scothbond Universal (SBU); G2 ? silane + SBU; G3 - Signum Zirconia Bond; G4 - Z-Prime Plus; G5 - MZ Primer. A cone of composite resin was built. The specimens were stored in 100% relative humidity with distilled water at 37?C for 48 h, and then submitted to a tensile bond strength test in a universal testing machine at a crosshead speed of 0.5 mm/min. The type of failure that occurred during the debonding procedure was analyzed. The mean results of the bond strength test (MPa) followed by the same letter represent no statistical difference by ANOVA and Tukey?s post-hoc test (p<0.05): G2=27.55a (?6.99), G4=23.71a (?5.65), G1=22.64a (?5.67), G5=13.64b (?5.49), G3=7.54c (?4.75). G2 and G4 exhibited predominantly cohesive failure in the resin composite cone. G1 and G5 had predominantly mixed failures, and G3 exhibited only adhesive failures. The SBU and Z-Prime Plus provided higher bond strength to zirconia ceramic.
(Artigo Os adesivos universais s?o t?o eficientes quanto um primer para a ades?o ? zirc?nia?) The aim of the study was to compare four universal adhesives and a primer on the bond strength to zirconia ceramic. Seventy-five zirconia ceramic samples were obtained and embedded in acrylic resin. The surface of the samples was polished with 600-, 800- and 1200-grit silicon carbide abrasive papers, and sandblasted with 50 ?m aluminum oxide particles for 5 s. The samples were divided into five groups (n=15): Single Bond Universal (SBU); G2 ? All Bond Universal (ABU); G3 ? Peak Universal Bond (PUB); G4 ? Ambar Universal (AU) e G5 ? Z-Prime Plus (ZP). A cone of composite resin was built on the adhesives and primer applied. The specimens were stored in distilled water at 37oC for 24 h, and then submitted to a tensile bond strength test in a universal testing machine at a crosshead speed of 0.5 mm/min. The type of failure that occurred during the debonding procedure was classified as adhesive, cohesive or mixed. The values of bond strength were analyzed by analysis of variance (ANOVA) followed by Tukey?s test (?=0.05). Means of bond strength (MPa) followed by the distinct letters represent statistical difference: G5=21,12a, G1=20,55a, G4=19,12ab, G2=14,22b, G3=8,45c. The failures were predominantly mixed in G1, G4 and G5, and predominantly adhesive in G2 and G3. SBU and AU adhesives obtained bond strength comparable to ZP.
Grégoire, Lucie. "Development of consensus primers for amplification of human papillomavirus DNA." Thesis, University of Ottawa (Canada), 1993. http://hdl.handle.net/10393/6582.
Full textWallace, Sarah Kate. "Molecular Gut Analysis of Carabids (Coleoptera: carabidae) using Aphid Primers." Thesis, Montana State University, 2004. http://etd.lib.montana.edu/etd/2004/wallace/WallaceS1204.pdf.
Full textMenon, Beena. "Role of metal alkoxides as primers in polymer/metal adhesion." Thesis, This resource online, 1990. http://scholar.lib.vt.edu/theses/available/etd-03122009-040723/.
Full textEade, Frederick Robert. "The graphic language of vernacular literacy primers in West Africa." Thesis, University of Reading, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359789.
Full textWalsh, Angela Barbara. "The performance of zinc silicate primers in 0.5M sodium chloride." Thesis, University of Newcastle Upon Tyne, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324927.
Full textSousa, Ana Beatriz Silva. "Efeito do uso de primers bioativos na interface dentina-adesivo." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/58/58131/tde-10082015-102247/.
Full textThe durability of composite resin restorations is one of the main points related to the success in aesthetic treatments. The longevity of adhesive restorations can be affected by various factors such as the materials, the clinical time of use and the dentin matrix degradation mediated by matrix metalloproteinases (MMPs). MMPs can affect the adhesive interface, compromising the bonding between dentin and adhesive. Thus, searching for better results on longevity of adhesive restorations, the aim of this study was to evaluate the effect of MMPs inhibitors (chlorhexidine - CHX 0.2% and doxycycline 3% ) and crosslinking agent (grape seed extract - GSE 15%) on resin composite bond strength (BS) of restorations submitted to load cycling. Class I cavities were performed in the central area of the exposed surface of 48 sound human molars. Then, it was divided into 4 groups (n=12) according to the surface treatment received after the acid etching: Group I - Control (Adhesive System - Adper Single Bond Plus - 3M ESPE); Group II - CHX 0.2% + Adhesive System; Group III - Doxycycline 3% + Adhesive System e Group IV - GSE 15% + Adhesive System. After restorative procedures (Filtek Supreme Ultra - 3M ESPE), the samples were stored in distilled water for 24 hours at 37°C and then subdivided into two subgroups (n=6): A - Control (stored in artificial saliva) and B - Submitted to load cycling (Coil Cycler, Proto-tech, 50 N, 1.5 Hz, 1 x 106 cycles). After load cycling, the specimens were cut into sticks (0.8 x 0.8 mm; n = 5 per teeth) and submitted to the microtensile test (Bisco) at 1 mm/min. The collagenolytic activity of MMP-2 under action of bioactive primers was analyzed using EnzChek Gelatinase/Colagenase Assay Kit (D-12054, Molecular Probes, Eugene, OR, EUA). The gelatinolytic activity of MMPs-2 and -9 after incubation with the bioactive primers was evaluated by gelatin zymography. The results of these two analyzes were presented by percentage of inhibition. In situ zymography was performed using fluorescence conjugated gelatin (EnzChek Gelatinase/Collagenase Assay Kit) as MMP substrate and evaluated using 40x magnification under fluorescence microscopy. Then the fluorescent photons were analyzed with an appropriate software (ImageJ). The results were subjected to statistical analysis (2-way ANOVA, Tukey, p<0.05) and the bioactive primers showed no significant influence on the dentin-adhesive BS, regardless the load cycling action. According to gelatin zymography, the collagenolytic activity demonstrated that the bioactive primers were able to inhibit MMPs activity and CHX was less efficient. In situ zymography analysis, showed that the bioactive primers decreased the activity of MMPs located in adhesive interface. The data also demonstrated increased gelatinolytic activity on Control group, being higher when submitted to load cycling, and which effect was not found in the groups treated with bioactive primers. It could be concluded that although the bioactive primers did not influenced the BS of adhesive interface, they demonstrated positive results on hybrid layer degradation.
Adhami, Siavash. "The development of water-based primers for coil coating applications." Thesis, University of Surrey, 2017. http://epubs.surrey.ac.uk/813181/.
Full textLima, Giana da Silveira. "A influência da água na composição de primers autocondicionantes experimentais." Universidade Federal de Pelotas, 2007. http://repositorio.ufpel.edu.br/handle/ri/2227.
Full textTo investigate the influence of different water concentrations in self-etching primers solvents on microtensile bond strength (μTBS) of an experimental adhesive system. Five experimental self-etching primers with 0, 5, 10, 20 and 40 water concentration (wt %) in solvent, were synthesized. Clearfil SE Bond (CSEB) was used as commercial adhesive. Sixty bovine incisive were randomize separated in six groups (n=20). Vestibular enamel was removed to expose the superficial coronal dentin, this surface was polished wet to create a standardized smear layer. After rinsing, water was removed, leaving the surface visibly dried. The dentin surfaces were etched with primer and air-dried, after coating adhesive resin was applied and photo-activated, following the composite resin restoration was accomplished. After storage for 24 hours, the specimens were sectioned with a refrigerated diamond saw at low-speed. Microtensile bond strength were measured and data were analyzed by one-way ANOVA/Tukey s test (α=0.05). Analysis of variance showed that primer composition was a significant factor for bond strength. There was not difference on bond strength of the primers with water concentration: 40% (53.9 ± 12.7 MPa), 20% (51.1 ± 11.5 MPa) and 10% (47.5 ± 11.4 MPa) and CSEB (52.44 ± 13.27 MPa). The group with 5% (38.6 ± 12.9 MPa) and 0% (31.5 ± 7.5 MPa), present similar bond strength amongst themselves but statistically smaller that the other groups. The water concentration, present in the primer solvent, exercise significant influence on the bond strength of this experimental self-etching adhesive system
Este estudo avaliou a influência de diferentes concentrações de água no solvente de um primer autocondicionante de um sistema adesivo dentinário experimental. Foram formulados cinco primers experimentais com diferentes concentrações, 0; 5; 10; 20; 40%, de água em massa e Clearfil SE Bond (CSEB) foi utilizado como referência comercial. Foram utilizados 60 incisivos bovinos, distribuídos aleatoriamente entre 6 grupos (n= 20). Na face vestibular dos dentes foi realizado desgaste com lixas até a exposição de dentina com lixa d água 600. O primer foi aplicado sobre a dentina previamente seca com papel absorvente, sendo aplicado jato de ar e em seguida o adesivo foi aplicado e foto-ativado com aparelho fotopolimerizador LED SDI Radii®. A restauração foi confeccionada utilizando 2 incrementos, fotoativados de acordo com as recomendações do fabricante. Após armazenagem por 24 horas em água destilada à 37°C, os dentes foram seccionados em cortadeira de precisão e a resistência de união mensurada, através de ensaio de microtração em uma máquina de ensaios mecânicos. Análise de Variância segundo um critério e teste complementar de Tukey foram utilizados na análise estatística (α=5%). Não houve diferença na resistência de união de P40 (53,9 ±12,7 MPa) P20 (51,1 ±11,5 MPa) e P10 (47,5 ±11,4 MPa) e CB (52,44 ±13,27 MPa). Os grupos P5 (38,6 ±12,9 MPa) e P0 (31.5 ±7.5 MPa) não apresentaram diferença estatística entre si, mas seus valores de adesão dentinária foram menores que os demais grupos (p<0,05). A resistência de união dentinária do sistema adesivo experimental testado sofre influência da concentração de água presente no solvente do primer autocondicionante
Spies, Sebastian [Verfasser]. "Mehrfache Chemische Primerverlängerung am 5'-Terminus eines Aminoterminalen Primers / Sebastian Spies." München : Verlag Dr. Hut, 2015. http://d-nb.info/1070123935/34.
Full textJavorsky, Joseph Frank. "Observing the Main Effects of Automotive Primers when Bonding to Polyvinylchloride." University of Toledo / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1353011316.
Full textChan, Brian Jeremy. "PCR Primers for The Detection of Propane and Butane-Oxidizing Microorganisms." DigitalCommons@CalPoly, 2011. https://digitalcommons.calpoly.edu/theses/456.
Full textRumzan, Ismael Hassen Aniff. "Impact behaviour of automotive multi-layer paint systems." Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325316.
Full textLi, Wei. "Protein-protein interaction specificity of immunity proteins for DNase colicins." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302033.
Full textArroyo, Cardona Gemma. "Valoració de la qualitat en els primers estadis de desenvolupament embrionari humà." Doctoral thesis, Universitat Autònoma de Barcelona, 2015. http://hdl.handle.net/10803/322799.
Full textSeveral strategies have been proposed for the selection of embryos for uterine transfer in human assisted reproduction. The possibility of choosing adequate embryos with high implantation potential will allow the reduction in the number of embryos transferred. This will lead to a decrease in the percentage of multiple pregnancies and its complications. The scoring criteria of embryo selection are based on serial morphological observations conducted on day 1 (during the assessment of fertilization and early cleavage), on days 2 and 3 (based on cleavage and blastomere fragmentation), on day 5, or on combinations of these criteria. Extending the culture of embryos to the blastocyst stage may also be a good option to select high quality embryos, as well as the determination of the chromosome constitution of the embryo. Unfortunately, not all cases are good candidates and benefit from extended culture. Pronuclear morphology assessment has been extensively described as a method to score zygotes. Also, some countries are only allowed to freeze zygotes and the selection of embryos at this stage is thus necessary. The objective was to evaluate the usefulness of pronuclear patterns as described by Tesarik and Greco, 1999 (patterns p 0-5) and Scott et al., 2000 (Z1-4) as well as the occurrence of early cleavage at 26h as predictors of embryo morphology, implantation potential and chromosome constitution. The first purpose was to relate pronuclear patterns (PN) and zygote cytoplasmic appearance and embryo morphology. On this sense, the usefulness of PN classifications for embryo selection was assessed. We observed that synchrony on polarization and number of nucleolar precursor bodies (NPB) were associated with good quality embryos. Pattern 4 zygotes were associated with small number of NPB developed into multinucleated embryos and poor quality embryos. No significant differences were found in the pregnancy rate between transfer of at least one good prognosis PN pattern and transfer of poor prognosis PN patterns. In order to evaluate the usefulness of pronuclear patterns as predictors of embryo chromosome constitution, up to 73 preimplantation genetic diagnosis/preimplantation genetic screening (PGD/PGS) cycles were analysed. The results show that the PN pattern using Tesarik’s and Scott’s classification systems is not related to the embryo developmental potential or its chromosome constitution. As regard to the relationship between PN pattern and embryo quality, the data obtained in the second study showed no correlation between both parameters. Although there were no significant differences when comparing the distribution of chromosomally normal and abnormal embryos with respect to embryo quality, such differences were observed when distinguishing between normal, aneuploid and polyploid embryos. The second objective was to analyse the correlation between early cleavage and embryo quality and chromosome constitution including 595 embryos from 96 PGS/PGD cycles. When clinical pregnancy rates per transfer were compared, statistically significant differences were observed between patients that had at least one early cleavage embryo and patients no EC. Statistically significant differences were found between EC, No PN and 2PN embryos at 26 h, good embryo quality at day 2 and in blastocyst rate in PGS cycles. These differences were not found in the PGD group. Early cleaved embryos exhibited less chromosome abnormalities than No PN and 2PN group in PGS and in PGD group. In conclusion, sequential assessment involving the evaluation of oocyte quality, the classification of PN patterns and embryo morphology allows a more accurate evaluation of embryos to be selected for transfer. Therefore, in the context of a PGD/PGS programme, the PN pattern cannot be used as a tool to predict embryo quality or chromosome status. Early cleavage has shown to correlate with embryo quality, with the capacity to develop up to blastocyst stage, as well as with euploid chromosome constitution.
Li, Longfei. "Study of Corrosion and Corrosion Inhibition of Chromate and Chromate-Free Primers." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1344896405.
Full textChan, Kar-mun. "Self-etching primers and adhesives : aspects of microtensile bond strength, ultrastructure and nanoleakage /." View the Table of Contents & Abstract, 2002. http://sunzi.lib.hku.hk/hkuto/record/B36358022.
Full textChan, Kar-mun, and 陳嘉敏. "Self-etching primers and adhesives: aspects of microtensile bond strength, ultrastructure and nanoleakage." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B42576374.
Full textDoan, Minh Tri Theerathavaj Srithavaj. "The effect of primers on bond strength of silicones to autopolymerizing acrylic resin) /." Abstract, 2006. http://mulinet3.li.mahidol.ac.th/thesis/2549/cd395/4736082.pdf.
Full textAhmad, M. Mursaleen Cheng Jianlin. "MUPrimer a tool for finding allele specific PCR-primers for homologous gene sequences /." Diss., Columbia, Mo. : University of Missouri--Columbia, 2009. http://hdl.handle.net/10355/6660.
Full textHarrison, Elizabeth. "Species-specific PCR primers for the rapid and reliable identification of yeast species." Thesis, University of Bath, 2006. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.434472.
Full textMiranda, Helen Lúcia da Cruz. "Geração de cDNAs, primers e caracterização estrutural do tegumento de sementes de soja." Universidade Federal de Pelotas, 2008. http://repositorio.ufpel.edu.br/handle/ri/1495.
Full textSoybean is one of the main world commodities and the seed is the key for high yields in this culture. Nevertheless, the soybean seed coat is the main modulator of seed quality, so, in this structure we could find answers for high seed quality production. The study goal is to identify structural contrasting traits, to obtain cDNA and to draw primers related to lignin synthesis and wax deposition in seed coat from two soybean genotypes. Plants were grown in greenhouse, flowers were identified and dated to follow the development after flowering until legumes were harvested (25, 30, 35, 40, 45, 50, 55 and 60 days). Seed coats were then analyzed in electronic microscopy and used to build a soybean seed coat cDNA library for permeability traits (lignin synthesis and wax deposition). In silico, analyzes were performed to obtain specific primers to detect soybean seed coat lignin and wax biosynthesis genes. According to the seed coat traits, we identified differences in the palisade cell thickness between genotypes. The protocol based on litio chloride was efficient to extract total RNA from both genotypes in all phases. The unidirectional cDNA multiplication was efficient for the generation of enough cDNA quantity for future projects. In silico analyzes allowed the development of 16 primers related to lignin and wax deposition biosynthesis in soybean seed coat.
A soja é uma das principais commodities mundiais e a semente o principal insumo para sua produção. O tegumento das sementes é o principal modulador da qualidade, podendo ser encontrado, nessa estrutura a resposta para a produção de sementes com caracteres superiores para vigor e viabilidade. O estudo teve como objetivo identificar características estruturais contrastantes, obter cDNA e desenhar primers relacionados à síntese de lignina e deposição de cera em tegumentos de sementes de soja de genótipos contrastantes em relação à permeabilidade CD 202 (permeável) e IAC Santa Maria 702 (semipermeável). As plantas foram cultivadas em casa de vegetação e a partir da primeira floração as flores foram marcadas para que o tempo de desenvolvimento de cada legume fosse identificado. Legumes com 25, 30, 35, 40, 45, 50, 55 e 60 dias de desenvolvimento foram coletados e os tegumentos extraídos e armazenados para extração de RNA. Tegumentos com 25, 40, 55 e 70 dias de desenvolvimento dos genótipos de soja CD 202 e IAC Santa Maria 702 também foram submetidos a Microscopia Eletrônica. Após a extração do RNA total, foram obtidos os RNAm, seguido do cDNA. Em paralelo foi realizada análise in silico para desenho de primers específicos de genes envolvidos na rota metabólica da lignina e na deposição de cera nas sementes. Foram detectadas diferenças na espessura da camada paliçádica dos tegumentos de sementes de soja dos genótipos CD 202 e IAC Santa Maria 702. O protocolo a base de cloreto de lítio foi eficiente para extrair RNA total de ambos os genótipos em todas as fases de coleta. A estratégia de multiplicação unidirecional do cDNA foi eficiente para geração de um volume considerável para o desenvolvimento de estudos futuros de expressão diferencial de genes. A análise in silico permitiu o desenvolvimento de 16 primers relacionados aos genes da lignina e da elongase.
Evander, Magnus. "Detection of human papillomavirus : a study of normal cells, cervical intraepithelial neoplasia and cancer of the uterine cervix." Doctoral thesis, Umeå universitet, Virologi, 1991. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-101351.
Full textDiss. (sammanfattning) Umeå : Umeå universitet, 1991, härtill 9 uppsatser.
digitalisering@umu
Nicholson, Thomas Peter. "Design and development of oligonucleotide probes for novel fungal polyketide synthase genes." Thesis, University of Bristol, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322607.
Full textOliveira, Fernanda Muller de [UNESP]. "Seleção in silico de sequências de dna espécie-específicas de Leishmania." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/144054.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Leishmaniasis, a disease caused by the protozoan Leishmania spp. is expanding in the world and affects millions of people annually, reaching 98 countries in tropical and subtropical regions, representing an important public health problem. Subspecies Leishmania and Viannia, represented by at least 22 species, are responsible for the three most common clinical manifestations of the disease in humans. Routinely, in endemic areas, serological diagnosis are employed. However, other parasitological and/or molecular techniques have high sensitivity to identify the genus Leishmania. The publication of Leishmania genomes have provided a better understanding of their composition and molecular markers to identify protozoan species and intra-specific variations, however some species are still underdiagnosed. However, the differentiation of Leishmania species in clinical samples still has limitations. Thus, in order to find the solution for this issue, we sought to design specific sequences for different species of Leishmania deposited in trypanosomatid genomic database, carrying out an in silico assay for verification of the selected sequences. The information generated in this study will constitute an interesting diagnostic platform, after proper validation with clinical samples
Oliveira, Fernanda Muller de. "Seleção in silico de sequências de dna espécie-específicas de Leishmania /." Araçatuba, 2015. http://hdl.handle.net/11449/144054.
Full textAbstract:Leishmaniasis, a disease caused by the protozoan Leishmania spp. is expanding in the world and affects millions of people annually, reaching 98 countries in tropical and subtropical regions, representing an important public health problem. Subspecies Leishmania and Viannia, represented by at least 22 species, are responsible for the three most common clinical manifestations of the disease in humans. Routinely, in endemic areas, serological diagnosis are employed. However, other parasitological and/or molecular techniques have high sensitivity to identify the genus Leishmania. The publication of Leishmania genomes have provided a better understanding of their composition and molecular markers to identify protozoan species and intra-specific variations, however some species are still underdiagnosed. However, the differentiation of Leishmania species in clinical samples still has limitations. Thus, in order to find the solution for this issue, we sought to design specific sequences for different species of Leishmania deposited in trypanosomatid genomic database, carrying out an in silico assay for verification of the selected sequences. The information generated in this study will constitute an interesting diagnostic platform, after proper validation with clinical samples
Orientador:Caris Maroni Nunes
Banca:Flávia Lombardi Lopes
Banca:Vania Lúcia Ribeiro da Matta
Banca:Rodrigo Martins Soares
Banca: Valéria Marçal Felix de Lima
Doutor
Martinez, Marcelo Luís Lombardi. "Aplicação de marcadores microssatélites na caracterização de recursos genéticos de Tabebuia roseo-alba conservados ex situ no banco de Germoplasma da floresta da USP de Ribeirão Preto." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/59/59139/tde-15012009-105304/.
Full textThe State of Sao Paulo, originally covered with semideciduous forests and Brazilian savannah (Cerrado), is nowadays almost completely covered with different cultures or pastures. Therefore only some small forests and Brazilian savannah fragments remain, pointing to a drastic loss of the rich forest genetic patrimony. The Region of Ribeirão Preto is one of the most devastated areas within the State of Sao Paulo. Especially areas that are located next to water sources and sugar cane plantations are affected. The original forests have nearly totally been fragmented and their actual extension has been reduced to about 2% of the original zone. The USP Forest Project has been implanted at the Sao Paulo University in Ribeirao Preto given the urgency to rescue the native forest species of the regional flora. There are 30 ha of heterogeneous reforestation and 45 ha that belong to the Germplasm Bank (BG-USP/RP). Tabebuia roseo-alba (White Tabebuia tree; Bignoniaceae) is one of the 44 species conserved at this Bank due to the fact that it is nowadays rarely encountered in the natural environment. There is na exigence of studies that aim at the agreement of its genetic diversity in the forest remnants and at the BG-USP/RP for the correct adoption of management and conservation strategies. Microsatellite markers are unquestionably indicated for this type of study because of their high information content, analytical robustness, transferability and easiness of genetic data attainment via PCR. This study aimed to analyze the genetic diversity of mother trees and their progeny individuals of T. roseo-alba and verify the maternity of these progeny individuals conserved at the BG-USP/RP, using 10 primer pairs SSR transferred from Tabebuia aurea. DNA was extracted from fresh leaves of all samples, the amplification and electrophoresis conditions were standardized and the polyacrilamyde gels stained with silver nitrate. Genetic parameters were estimated using the programs GDA 1.0, FSTAT 2.9.3, Cervus 3.0 and Structure 2.2.3. There was a 90% success in the amplification of the microsatellite regions for 10 loci SSR, but 8 loci SSR had been used in this study. These loci are in linkage equilibrium and presented an average PIC value highly informative (0.745). A high allelic richness was observed for the mother trees (85 alleles) and the progeny individuals at the Bank (96 alleles) and also a high genetic diversity (0.746 e 0.775, respectively), being that the Ho average was smaller than the He average, evidencing a heterozigote deficit. The fixation index for the mother trees of T. roseo-alba was high (Fis =0.638) and significantly different from zero (P < 0.05)), suggesting the performance of some factor that caused endogamy such as crossings between related individuals, self-fertilization, the Wahlund effect and the null alleles presence segregating in these loci. Additionally, the progeny individuals also presented a high Fis value (0.696), indicating that the T. roseo-alba mating system might be the main factor for the high endogamy coefficient. The joint analysis of these loci in the mother trees and progeny individuals presented high paternity exclusion probabilities, confirming that this loci battery has a high potential for paternity/maternity analysis studies in T. roseo-alba. Maternity analyses showed that only 62 % of the T. roseo-alba progeny individuals at the BG-USP/RP have their maternal origin identified. The supplying with T. roseo-alba seeds for reforestation programs will not be endangered because 95.3 % of the mother tree alleles have been transferred to the progeny individuals at the BG-USP/RP, contributing to the ex situ conservation of this important forest species.
Vieira, Helena Henriques. "Avaliação da universalidade de primers de marcadores moleculares para aplicação na classe Chlorophyceae (Chlorophyta)." Universidade Federal de São Carlos, 2014. https://repositorio.ufscar.br/handle/ufscar/2108.
Full textFinanciadora de Estudos e Projetos
The polifasic taxonomy approach became indispensable in classification of living beings, especially microorganisms, such as Chlorophyceae. This class is compound of very distinct organisms most microscopic, unicellular, colonials, filamentous, and the classification and identification is many times hampered due the lack of structures observable that helps the discrimination. Some habits, reproduction, the occurrence of cryptic species are some factors that can lead to forms that make difficult the taxonomy of these organisms. Besides the traditional morphology, the molecular biology allows a robust concerning the classification of the studied organism. Associated to a better classification, the search for faster and more practical methods of identification, as DNA Barcode, became intensified and many molecular markers are available in literature to be tested. For some groups of animals, brown algae, red algae and diatoms the marker COXI is considered an official DNA Barcode. However other groups, as Chlorophycea, are under constant investigation, due the fact that molecular marker that can be used satisfactory as DNA Barcode have not been found yet. Plastid markers as tufA, rbcL and nuclear as ITS are some of the most proposed to be applied in green algae, often combined with each other or other markers. However, for act as a molecular marker, it is necessary to be amplified by primers broadly applied. The present study has tested primers for tufA, ITS and rbcL marker, available in literature and already in use in other groups, looking for universality in Chlorophyceae. We also tested proposed universal primers for Chlorophyta (UCP4). Moreover, preliminary analyses have been performed for application of the markers as barcode in the class. The primers used for tufA marker showed universality in Chlorophycea amplifying for all 22 strains tested and sequencing have failed only for Oedogonium strain. From the rbcL marker, the primer pair GrbcL has amplified 12 and sequenced 7 of 22 strains and rbcLFP which one the reverse primer was designed in this study amplified and sequenced 16 lineages. Primers from the ITS marker amplified only 5 lineages and UCP4 primers have not amplified any strain. Beside the universality found for tufA primers, Analysis with the obtained sequences showed that the tufA gene is a promising molecular marker to be applied in Chlorophyceae.
A abordagem polifásica de taxonomia se tornou indispensável na classificação especialmente de microorganismos, como os da classe Chlorophyceae. Esta classe é composta de organismos muito distintos, sendo a maioria microscópica, apresentando formação do talo unicelular, colonial e filamentosa e a classificação e identificação dos representantes desta classe são muitas vezes dificultadas pelo fato de não possuírem estruturas observáveis em microscópio usadas para discriminação. Alguns hábitos e tipos de reprodução, a ocorrência de espécies crípticas são alguns fatores que podem resultar em formas que também dificultam a taxonomia destes organismos. Associada a uma classificação mais robusta, a procura por métodos mais rápidos e práticos, como o DNA Barcode, se intensificou e muitos marcadores moleculares já se encontram disponíveis na literatura para serem testados. Para alguns grupos de animais, algas pardas, algas vermelhas e diatomáceas o marcador COXI é considerado oficial como DNA Barcode. Entretanto, outros grupos como Chlorophyceae este marcador não é aplicável devido a sua natureza conservada e, portanto, estão sob constante investigação, devido ao fato de que ainda não foram encontrados marcadores que possam ser aplicados satisfatoriamente como DNA Barcode. Marcadores plastidiais como tufA, rbcL e nucleares como o ITS são alguns dos mais aplicados em estudos com algas verdes, muitas vezes combinados entre si ou com outros marcadores. Entretanto para que figurem como marcadores moleculares devem ser amplificados por primers que possam ser aplicados de maneira ampla. Assim, o presente estudo teve como objetivo testar primers para os marcadores tufA, ITS, rbcL , disponíveis na literatura e já aplicados com outros grupos, buscando a universalidade destes primers também em Chlorophyceae. Foi testado também um par de primers proposto como universal para Chlorophyta (UCP4). Os primers para o gene tufA mostraram universalidade para a classe amplificando todas as 22 cepas testadas com falha no seqüenciamento apenas para a cepa de Oedogonium sp.Os primers para o gene rbcL, GrbcL, amplificou 12 e seqüenciou satisfatoriamente 7 cepas das 22 utilizadas, e o par rbcLFP, o qual teve um de seus iniciadores desenhado neste estudo, amplificou e seqüenciou 16 cepas. Primers para o gene ITS amplificaram 5 cepas com bom seqüenciamento de apenas 3 e o par de primers UCP4 não amplificou nenhuma cepa. Além da universalidade observada do par de primers para o gene tufA, análises com as seqüências obtidas mostraram que este gene se mostrou um promissor candidato a marcado molecular para aplicação na classe Chlorophyceae.
Good, J. C. "The study of enzymes and primers involved in the initiation of chains of glucans." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375257.
Full textJackson, Constanza. "Synthesis of 2’ Modified Primers to Characterize Extension Events by Mutant Taq DNA Polymerases." Scholarship @ Claremont, 2015. http://scholarship.claremont.edu/scripps_theses/592.
Full textFerreira, Henrique. "Primers desenvolvidos atraves de hibridização subtrativa e RAPD para a detecção de xylella fastidiosa." [s.n.], 1988. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317425.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Mestrado
Destefano, Ricardo Henri Rodrigues. "Detecção e identificação de Metarhizium anisopliae em larvas de Diatraea saccharalis por Primers específicos." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-15072003-141752/.
Full textThe ITS rDNA have been used as an important molecular tool for fungi identification. In this study the ITS1 - 5.8S ITS2 rDNA regions were analyzed in different species of the entomopathogenic fungus Metarhizium including M. anisopliae, M. album and M. flavoviride, in order to construct specific primers for their detection and identification within infected Diatraea saccharalis larvae. The amplification of this region yielded a unique fragment of approximately 540 bp for E9, B/Vi and C, and 600 bp for strain 14 of M. anisopliae var. anisopliae; of 650 bp for M. album and 600 bp for M. flavoviride. The PCR products were digested with the different restriction endonucleases Afa I, Alu I, Dde I, Hae III, Hpa II and Sau 3A I; and the PCR-RFLP profiles showed clear differences amongst the species. The sequencing of the ITS-5.8S rDNA regions allowed for the design of specific primers for M. anisopliae var. anisopliae. The amplification was not observed with M. album, M flavoviride and Beauveria bassiana strains. DNAs extracted from infected larvae by E9 and C strains from Brazil and the strain 14 from Australia in individual bioassays were tested using previously designed specific primers. In all experiments, the fungus was detected after 48 hours of post-inoculation. This molecular technique allows a fast and secure detection and identification of the entomopathogen in bioassays, in pest management programs and epizootiology.
Guimarães, Guilherme Zdradk. "Influência da concentração e tipo de monômero ácido na adesão de primers à zircônia." Universidade Federal de Pelotas, 2011. http://repositorio.ufpel.edu.br/handle/ri/2273.
Full textIn this study the effect of concentration and type of acidic monomer on the bond strength (BS) of experimental primers to zirconia before and after aging simulation was investigated. Primers were formulated from the mixture of the monomers urethane dimethacrylate and 2-hidroxyethyl methacrylate with the phosphate monomer (PAM) 1,3-glycerol dimethacrylate phosphate or the carboxylic monomer (CAM)mono-2-(methacryloyloxy)ethyl maleate, and ethanol. In each primer, PAM or CAM were be added at concentrations of 10, 20, 40 or 60 mass%. Degree of conversion (DC) of the primers was evaluated by infrared spectroscopy (n=5). For the BS test, sintered yttria-stabilized zirconia blocks (Zircon-CAD, Angelus) were used. The primers were applied to the ceramic and cylinders of dual-cure resin cement (Eco-Link, Ivoclar Vivadent) built-up on the surfaces for the shear BS test (n=20). In the control group, adhesion of the cylinders will be carried out without using primer. In half the number of specimens in each group, the BS test was carried out after 24h on a mechanical testing machine. In order to simulate aging and evaluate the stability of the zirconia bonds, the other half the number of specimens was submitted to 3000 thermal cycles before the BS test. The failure modes were classified under a 40× magnification.DC data were submitted to one-way ANOVA for each monomer; BS data were submitted to two-way ANOVA for each storage condition. The Student-Newman-Keuls post-hoc test was used. BS comparison between 24h and after thermalcycling was carried out using t-tests. (Non-)linear regression analyses were used to investigate the relationship between acidic monomer concentration and DC or BS (α=0.05 for all analyses). Increase in acidic monomers concentration was associated with a linear decrease in DC. The BS for PAM-based primers was higher than CAM-based materials for all groups except 10% at 24h. For PAM, the primer with 40% of acidic monomers yielded higher BS than all the other primers, either at 24h or after thermalcycling. For CAM, the BS results were at 24h were 10% > 60% > 40% > 20%, while after thermalcycling the primer with 60% of acidic monomers generally showed higher BS. All PAM groups except 10% showed lower BS after the thermal cycles, while 10% was the only concentration that showed lower BS after thermalcycling for CAM. Increased PAM concentration had a significant relationship with the zirconia bonds according to a peak model, while increase in CAM content had a polynomial inverse relationship. Regression models after thermalcycling were not significant. The only group that showed mixed failures was PAM 40%, while the other groups failed adhesively. In conclusion, both the acidic monomer type and concentration have a significant role on the adhesion to zirconia. The primer with 40% of PAM showed the best performance on bonding to zirconia
Neste estudo avaliou-se o efeito da concentração e tipo de monômero ácido na resistência de união (RU) de primers experimentais à zircônia antes e após simulação de envelhecimento. Primers foram formulados a partir da mistura dos monômeros dimetacrilato de uretano e metacrilato de 2-hidroxietila com o monômero ácido fosfórico (MAF) dimetacrilato de glicerol-fosfato ou o monômero ácido carboxílico (MAC) maleato de mono(2-metacriloiloxi)etila, além de etanol. Em cada primer, MAF ou MAC foram adicionados nas concentrações 10, 20, 40 ou 60% em massa. O grau de conversão (GC) dos primers foi avaliado por espectroscopia infravermelha (n=5). Para o teste de RU, blocos sinterizados de zircônia estabilizada por ítria (Zircon-CAD, Angelus) foram utilizados. Os primers foram aplicados na superfície da cerâmica e nesta obtidos cilindros de cimento resinoso dual (Eco-Link, Ivoclar Vivadent) para teste de RU ao cisalhamento (n=20). Em metade do número de espécimes de cada grupo, o teste de RU foi realizado após 24h, em máquina de ensaios mecânicos.Para simular envelhecimento e avaliar a estabilidade da união à zircônia, a outra metade do número de espécimes foi submetida a 3000 ciclos térmicos antes do teste de RU. Os modos de falha foram classificados sob aumento de 40×. Os dados de GC foram submetidos a Análise de Variância de 1 critério para cada monômero; os dados de RU foram submetidos a Análise de Variância de 2 critérios para cada condição de armazenamento. O teste post-hoc de Student-Newman-Keuls foi utilizado. Comparação de RU entre 24h e após termociclagem foi realizada com testes-t. Análises de regressão (não-)linear foram utilizadas para investigar a relação entre concentração de monômero ácido e GC ou RU (α=0,05 para todas as análises). Aumento na concentração de monômeros ácidos foi associado a diminuição linear no GC. A RU de primers à base de MAF foi maior que materiais à base de MAC para todos os grupos exceto 10% a 24h. Para o MAF, o primer com 40% de monômeros ácidos gerou RU maior que todos os demais, a 24h ou após termociclagem. Para o MAC, os resultados de RU a 24h foram 10% > 60% > 40% > 20%, enquanto após ciclagem o primer com 60% de monômeros ácidos em geral mostrou maior RU. Todos os grupos do MAF exceto 10% mostraram RU menor após os ciclos térmicos, enquanto 10% foi a única concentração que apresentou menor RU após a ciclagem para o MAC. Aumento na concentração de MAF teve relação significativa com a adesão á zircônia de acordo com um modelo de pico, enquanto aumento no conteúdo de MAC teve uma relação polinomial inversa.Os modelos de regressão após termociclagem não foram significativos. O único grupo que teve falhas mistas foi o MAF 40%, enquanto todos os outros falharam adesivamente. Como conclusão, tanto o tipo quanto a concentração de monômeros ácidos têm papel significativo na adesão de primers à zircônia. O primer com 40% de MAF mostrou o melhor desempenho na adesão à zircônia
Xu, Hong. "Magnesium Alloy Particulates Used as Pigments in Metal-Rich Primer System for AA2024 T3 Corrosion Protection." Diss., North Dakota State University, 2010. https://hdl.handle.net/10365/28378.
Full textAir Force Office of Scientific Research (AFOSR) (Grant No. 49620-02-1-0398)
Xu, Hong. "Magnesium Alloy Particulates used as Pigments in Metal-Rich Primer System for AA2024 T3 Corrosion Protection." Diss., North Dakota State University, 2011. https://hdl.handle.net/10365/28838.
Full textAir Force Office of Scientific Research (Grant No. 49620-02-1-0398)
Kesanakurti, S. S. V. Prasada Rao. "Development and application of single or low copy universal primers from the plant nuclear genome." Thesis, University of Reading, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.435691.
Full textOrgon, Casey Roy. "Electrochemical Investigation of Powder Coatings and Their Application to Magnesium-Rich Primers For Corrosion Protection." Thesis, North Dakota State University, 2015. https://hdl.handle.net/10365/27814.
Full textUS Air Force under grant no. FA7000-13-2-0025
Bonatto, Liliane da Rocha [UNESP]. "Biocompatibilidade de primers e adesivo utilizados na confecção de próteses maxilofaciais implantorretidas: análise in vitro." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/134194.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A prótese bucomaxilofacial implantorretida pode ser suportada por pele ou por mucosa. Subprodutos dos materiais utilizados na confecção destas próteses podem atuar como irritantes ou causadores de reações alérgicas a tais tecidos. A proposta do presente estudo foi avaliar a citotoxicidade de primers e adesivo utilizados na confecção de próteses maxilofaciais retidas por implantes, por meio da análise da proliferação celular e da produção de citocinas pró-inflamatórias e de proteínas de matriz extracelular por queratinócitos humanos. Foram confeccionadas 28 amostras de resina e silicone, em forma de discos (10 x 1 mm), unidas ou não pela aplicação de primer e/ou adesivo. Estas amostras foram distribuídas em 7 grupos: Resina (R), Silicone (S), Resina + Silastic Medhical Adhesive Type A + Silicone (RAS), Resina + DC 1205 primer + Silicone (RDCpS), Resina + Sofreliner primer + Silicone (RSpS), Resina + DC 1205 primer + Silastic Medhical Adhesive Type A + Silicone (RDCpAS) e Resina + Sofreliner primer + Silastic Medhical Adhesive Type A + Silicone (RSpAS). Os extratos dos materiais testados foram preparados colocando-se quatro amostras de cada grupo experimental em tubos de ensaio contendo 9 mL de meio de cultura DMEM (Dulbecco's Modified Eagle's) e incubados a 37°C por 24 horas. Após o período de incubação, a citotoxicidade dos extratos foi avaliada pelo ensaio de MTT em cultura de queratinócitos humanos (HaCaT). Foi avaliada, também, a produção das citocinas IL-1, IL-6 e TNF-α e a quimiocina MIP-1α por meio do ensaio ELISA (Ensaio Imunoabsorbente de Ligação Enzimática). Também foi avaliada a expressão de RNAm para MMP-9, TGF-β e COL-IV por meio da técnica de RT-PCR em tempo real (Reação em Cadeia da Polimerase em Tempo Real). Os dados obtidos foram submetidos à Análise de Variância (ANOVA), seguido...
The implant-retained maxillofacial prosthesis can be supported by skin and mucosa. The sub-products produced by the materials used to fabricate these prostheses may act as an irritant factor and cause allergy in these tissues. The aim of this study was to evaluate the cytotoxic effect of primers and adhesive used to bond the acrylic resin and the facial silicone during implant-retained maxillofacial prosthesis fabrication, through the analysis of the cell proliferation, and the production of proinflammatory cytokines and extracellular matrix proteins by keratinocytes. A total of 28 round shape samples (10 x 1 mm) made of resin and silicone bonded or not with primer and adhesive was fabricated. Samples were divided into 7 groups: Resin (R), Silicone (S), Resin + Silastic Medical Adhesive Type A + Silicone (RAS), Resin + DC 1205 primer + Silicone (RDCpS), Resin + Sofreliner primer + Silicone (RSpS), Resin + DC 1205 primer + Silastic Medical Adhesive Type A + Silicone (RDCpAS), and Resin + Sofreliner primer + Silastic Medical Adhesive Type A + Silicone (RSpAS). Extracts of tested materials were prepared setting four samples of each experimental group in Falcon tube with 9mL of medium (Bulbecco's Modified Eagle's) and incubated at 37°C for 24 hours. After incubation period, the extract cytotoxicity was evaluated by an assay of cell survival/proliferation (MTT test) in cultures of human keratinocytes (HaCaT). The levels of IL-1, IL-6 and TNF-α and the chemokine MIP-1α were evaluated by ELISA (Enzyme-Linked Immunoabsorbent Assay). The mRNA expression for MMP-9, TGF-β and collagen type IV were analyzed by the RT-PCR (Real time polymerase chain reaction). Data were submitted to the analysis of variance with Bonferroni post-tests (p<0.05). The results showed increased cell proliferation for the RAS group. The RDCpS group showed the highest...