Academic literature on the topic 'Primer designs'
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Journal articles on the topic "Primer designs"
Ahern, Kevin, and Primer Premier. "Premier Primer Designs." Science 286, no. 5439 (October 15, 1999): 433.1–433. http://dx.doi.org/10.1126/science.286.5439.433a.
Full textSINGH, KAVITA, PRITI GUPTA, and ROOPA SHIVASHANKAR. "Primer of Epidemiology IV. Study designs II: Interventional or experimental designs." National Medical Journal of India 34 (January 29, 2022): 228–31. http://dx.doi.org/10.25259/nmji_373_19.
Full textBauman, Sheri. "Using Comparison Groups in School Counseling Research: A Primer." Professional School Counseling 9, no. 4 (January 2006): 2156759X0500900. http://dx.doi.org/10.1177/2156759x0500900419.
Full textBaker, Timothy B., Stevens S. Smith, Daniel M. Bolt, Wei-Yin Loh, Robin Mermelstein, Michael C. Fiore, Megan E. Piper, and Linda M. Collins. "Implementing Clinical Research Using Factorial Designs: A Primer." Behavior Therapy 48, no. 4 (July 2017): 567–80. http://dx.doi.org/10.1016/j.beth.2016.12.005.
Full textLedford, Jennifer R., Erin E. Barton, Katherine E. Severini, and Kathleen N. Zimmerman. "A Primer on Single-Case Research Designs: Contemporary Use and Analysis." American Journal on Intellectual and Developmental Disabilities 124, no. 1 (January 1, 2019): 35–56. http://dx.doi.org/10.1352/1944-7558-124.1.35.
Full textHavstad, Suzanne L., and George W. Divine. "Biostatistical Analysis: A Primer for Clinical Exercise Physiology, Part 1." Journal of Clinical Exercise Physiology 7, no. 3 (September 1, 2018): 63–69. http://dx.doi.org/10.31189/2165-6193-7.3.63.
Full textPrabhakaran, Poornima, and Roopa Shivashankar. "Primer of Epidemiology 3: An overview and observational study designs." National Medical Journal of India 33, no. 5 (2020): 291. http://dx.doi.org/10.4103/0970-258x.317472.
Full textO'Brien, Ralph G., and Mary K. Kaiser. "MANOVA method for analyzing repeated measures designs: An extensive primer." Psychological Bulletin 97, no. 2 (1985): 316–33. http://dx.doi.org/10.1037/0033-2909.97.2.316.
Full textNuryady, Moh Mirza, H. Husamah, Fuad Jaya Miharja, Iin Hindun, and P. Patmawati. "Desain dan Optimasi Primer Gen Pengkode MRPA Trypanosoma evansi dan Penerapan pada Pembelajaran Biologi Molekuler." Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika 4, no. 2 (July 2, 2020): 211. http://dx.doi.org/10.36312/e-saintika.v4i2.217.
Full textJoseph, Anjali, Matthew H. E. M. Browning, and Shan Jiang. "Using Immersive Virtual Environments (IVEs) to Conduct Environmental Design Research: A Primer and Decision Framework." HERD: Health Environments Research & Design Journal 13, no. 3 (May 21, 2020): 11–25. http://dx.doi.org/10.1177/1937586720924787.
Full textDissertations / Theses on the topic "Primer designs"
Mann, Tobias. "A thermodynamic approach to PCR primer design." Saarbrücken VDM Verlag Dr. Müller, 2007. http://d-nb.info/988796201/04.
Full textMerkley, John. "A sustainable design primer for students of architecture." Virtual Press, 2005. http://liblink.bsu.edu/uhtbin/catkey/1327785.
Full textDepartment of Architecture
Berg, Emily Katherine. "Thermodynamics of λ-PCR Primer Design and Effective Ribosome Binding Sites." Thesis, Virginia Tech, 2019. http://hdl.handle.net/10919/89900.
Full textMaster of Science
Recombinant DNA technology has been used to genetically enhance organisms to produce greater amounts of a product already made by the organism or to make an organism synthesize a new product. Genes are commonly modified in organisms using cloning practices which typically involves inserting a target gene into a plasmid and transforming the plasmid into the organism of interest. A new cloning process developed in the Senger lab, λ-PCR, improves the cloning process compared to other methods due to its use of relatively inexpensive materials and high efficiency. A primary goal of this study was to develop a procedure for λ-PCR primer design that allows for accurate use of the cloning method. Additionally, this study investigated the use of synthetic ribosome binding sites to control and improve expression of proteins cloned into an organism. Ribosome binding sites are sequences located upstream of the gene that increase the molecule’s affinity for the rRNA sequence on the ribosome, bind to the ribosome just upstream of the beginning of the gene, and initiate expression of the gene. Tools have been developed that create synthetic ribosome binding sites designed to produce specific amounts of protein. For example, the tools can increase or decrease expression of a gene depending on the application. These tools, the Salis Lab RBS Calculator and NUPACK, were used to design and evaluate the effects of the synthetic ribosome binding sites. Additionally, a new method was created to design synthetic ribosome binding sites since the methods used during the design process yielded inaccuracies. Each strain of E. coli contained the same gene, a cyan fluorescent protein (CFP), but had different RBS sequences located upstream of the gene. Expression of CFP was controlled via induction, meaning the addition of a particular molecule, IPTG in this system, triggered expression of CFP. Each of the CFP strains were tested with a variety of v conditions in order to find the conditions most suitable for protein expression; the variables tested include: induction time, IPTG (inducer) concentration, and temperature. Media was also tested for the cell-free systems, meaning the strains were grown overnight for 18 hours and lysed, a process where the cell membrane is broken in order to utilize the cell’s components for protein expression; the cell lysate was resuspended in new media for the experiments. ANOVA and multiple linear regression revealed IPTG concentration, induction time, and media to be significant factors impacting protein expression. This analysis also showed each CFP strain did not perform as the RBS Calculator predicted. Modeling each strain’s CFP expression using the RBS-rRNA binding strengths and secondary structures present in the RBS allowed for the creation of a new model for predicting and designing RBS sequences.
Yang, Szu-Wei. "Design of a toroidal thermoacoustic prime mover." Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 1995. http://handle.dtic.mil/100.2/ADA303437.
Full textThesis advisor(s): Anthony A. Atchley, Thomas J. Hofler. "June 1995." Includes bibliographical references. Also available online.
Baldwin, D. Bruce. "Minisatellite PCR primer design for the determination of parentage in Misumenoides formosipes." Virtual Press, 1999. http://liblink.bsu.edu/uhtbin/catkey/1136715.
Full textDepartment of Biology
Wozniak, Amanda Victrix Allen. "A systematic and extensible approach to DNA primer design for whole gene synthesis." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/37059.
Full textIncludes bibliographical references (leaves 93-96).
The future of synthetic biology research hinges upon the development of accurate and inexpensive whole gene synthesis technologies. Recent advances in the purification of solid-phase manufactured oligonucleotides make it possible to manufacture whole genes by polymerase chain reaction methods. Yet, despite the improvement in laboratory methods, whole gene synthesis is not rapidly progressing because most gene design software takes an excessively naive approach to the complex problem of designing component oligonucleotides for whole gene synthesis. The synthetic biology community needs a flexible, robust and optimal primer design tool. We present the software design for a tool which designs oligonucleotides that are compatible with a wide variety of oligo purification and whole gene assembly protocols. Our design strategy uses physical sequence feature identification, optimal artificial intelligence search techniques, and sequence optimisation via intelligent codon substitution to produce near-optimal oligonucleotide arrays. We address all aspects of the oligonucleotide design problem, from physical constraints to the computational overhead involved in searching for an optimal solution, and provide an extensive set of data structures and algorithms.
by Amanda Victrix Allen Wozniak.
M.Eng.
Thompson, Denis. "Finding homologous genes with primers designed using evolutionary models." NCSU, 2003. http://www.lib.ncsu.edu/theses/available/etd-10232003-122816/.
Full textRocha, Kliger Kissinger Fernandes. "Um sistema computacional para diagnosticar viroses de plantas usando a t?cnica de PCR com constru??o de primers esp?cie-espec?ficos." Universidade Federal do Rio Grande do Norte, 2005. http://repositorio.ufrn.br:8080/jspui/handle/123456789/15442.
Full textCoordena??o de Aperfei?oamento de Pessoal de N?vel Superior
It proposes a established computational solution in the development of a software to construct species-specific primers, used to improve the diagnosis of virus of plant for PCR. Primers are indispensable to PCR reaction, besides providing the specificity of the diagnosis. Primer is a synthetic, short, single stranded piece of DNA, used as a starter in PCR technique. It flanks the sequence desired to amplify. Species-specific primers indicate the well known region of beginning and ending where the polymerase enzyme is going to amplify on a certain species, i.e. it is specific for only a species. Thus, the main objective of this work is to automatize the process of choice of primers, optimizing the specificity of chosen primers by the traditional method
Prop?e-se uma solu??o computacional baseada no desenvolvimento de um software para construir primers esp?cie-espec?ficos, usados para melhorar o diagn?stico de viroses de planta por PCR. Primers s?o indispens?veis ? rea??o PCR, al?m de proporcionar a especificidade do diagn?stico. Um primer ? um fragmento de DNA sint?tico, curto e de fita simples, utilizado como um iniciador na t?cnica PCR que flanqueia a seq??ncia que se deseja amplificar. Primers esp?cie-espec?ficos s?o primers que s? indicam a regi?o bem conhecida de in?cio e t?rmino onde a enzima polimerase vai amplificar, de uma determinada esp?cie, ou seja, ? espec?fica para somente uma esp?cie. Assim, o objetivo principal deste trabalho ? automatizar o processo de escolha de primers, otimizando a especificidade dos primers escolhidos pelo m?todo tradicional
Ma, Jing S. M. Massachusetts Institute of Technology. "Recovery of primal solution in dual subgradient schemes." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/41729.
Full textIncludes bibliographical references (p. 97-99).
In this thesis, we study primal solutions for general optimization problems. In particular, we employ the subgradient method to solve the Lagrangian dual of a convex constrained problem, and use a primal-averaging scheme to obtain near-optimal and near-feasible primal solutions. We numerically evaluate the performance of the scheme in the framework of Network Utility Maximization (NUM), which has recently drawn great research interest. Specifically for the NUM problems, which can have concave or nonconcave utility functions and linear constraints, we apply the dual-based decentralized subgradient method with averaging to estimate the rate allocation for individual users in a distributed manner, due to its decomposability structure. Unlike the existing literature on primal recovery schemes, we use a constant step-size rule in view of its simplicity and practical significance. Under the Slater condition, we develop a way to effectively reduce the amount of feasibility violation at the approximate primal solutions, namely, by increasing the value initial dual iterate; moreover, we extend the established convergence results in the convex case to the more general and realistic situation where the objective function is convex. In particular, we explore the asymptotical convergence properties of the averaging sequence, the tradeoffs involved in the selection of parameter values, the estimation of duality gap for particular functions, and the bounds for the amount of constraint violation and value of primal cost per iteration. Numerical experiments performed on NUM problems with both concave and nonconcave utility functions show that, the averaging scheme is more robust in providing near-optimal and near-feasible primal solutions, and it has consistently better performance than other schemes in most of the test instances.
by Jing Ma.
S.M.
Conover, Susan (Susan Teresa). "Prime areas for improvement in skin cancer detection and how technology can help." Thesis, Massachusetts Institute of Technology, 2015. http://hdl.handle.net/1721.1/105308.
Full textCataloged from PDF version of thesis.
Includes bibliographical references (pages 138-148).
About 5 million cases of skin cancer will be diagnosed in the United States in 2015, making skin cancer the most common cancer diagnosis in the United States. About 13,000 Americans will die from skin cancer in 2015. Often skin cancers are diagnosed at later stages, are expensive to treat, and result in fatalities. For melanoma, responsible for 75% of skin cancer deaths, the overall 5-year survival rate is 98% for skin lesions detected in their early stages, and this survival rate drops to 16% after the disease has spread to other organs. If these fatal skin cancers were detected earlier they would cost less to treat and result in better patient outcomes. There is no single resource available that maps the full state of the skin cancer care delivery, and most current views are colored by a stakeholder's perspective. We connected with stakeholders at different levels of the skin cancer care delivery system to create an overall picture of the system's current state and to identify gaps in care. We interviewed 9 skin cancer patients, 8 primary care physicians, and 9 dermatologists. Through this research, we discovered that the structure of how skin cancer care is delivered promotes opportunities to miss skin cancers and includes many barriers between initial cancer suspicion and disease diagnosis. Frequently patients do not evaluate themselves for skin cancer, primary care physicians have low accuracy in identifying skin cancers, and dermatologists manage a very small portion of the population who develop skin cancers. At a higher level, feedback between patients and physicians is frequently lost in the system, physicians are not accountable for patient outcomes, and patient health is not supported by the system until the patient identifies a health issue and acts to remedy the issue. To close these system gaps, we identified technologies, including micro-biopsies and electrical impedance spectrometry, which could be used to improve rates of skin cancer identification and promote better patient health outcomes. Additionally, we recommend physicians find a way to collaborate on cases, identify their own weaknesses in assessment, and capture patient outcomes to relay incorrect assessments to other physicians to improve future patient care.
by Susan Conover.
S.M. in Engineering and Management
Books on the topic "Primer designs"
Yee, Rendow. Architectural graphics primer. San Francisco: City College of San Francisco, Department of Architecture, 1990.
Find full textLund, H. Gyde. A primer on stand and forest inventory designs. [Washington, D.C.?]: U.S. Dept. of Agriculture, Forest Service, 1989.
Find full textJuhani, Pallasmaa, ed. Understanding architecture: A primer on architecture as experience. London: Phaidon Press Limited, 2012.
Find full textEllen, Cheever, and National Kitchen and Bath Association (U.S.), eds. The National Kitchen & Bath Association presents kitchen basics: A training primer for kitchen specialists. 4th ed. Hackettstown, NJ: National Kitchen & Bath Association, 1995.
Find full textCatanzaro, Thomas E. Design it right: A pre-architect primer for planning your veterinary facility flow. 4th ed. Lakewood, Colo: American Animal Hospital Association Press, 2006.
Find full textAn Eames primer. New York: Universe Pub., 2001.
Find full textHester, Randolph T. Community design primer. Mendocino, Calif: Ridge Times Press, 1990.
Find full textBasu, Chhandak, ed. PCR Primer Design. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-1799-1.
Full textBasu, Chhandak, ed. PCR Primer Design. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2365-6.
Full textYuryev, Anton, ed. PCR Primer Design. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-528-2.
Full textBook chapters on the topic "Primer designs"
Berry, Kenneth J., Janis E. Johnston, and Paul W. Mielke. "Completely-Randomized Designs." In A Primer of Permutation Statistical Methods, 257–313. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-20933-9_8.
Full textBerry, Kenneth J., Janis E. Johnston, and Paul W. Mielke. "Randomized-Blocks Designs." In A Primer of Permutation Statistical Methods, 315–59. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-20933-9_9.
Full textConsolvo, Sunny, Frank R. Bentley, Eric B. Hekler, and Sayali S. Phatak. "Answering “Did it work?”: A Primer to Experimental Designs to Test for Change." In Mobile User Research, 101–32. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-031-02485-6_5.
Full textChristensen, Henrik, and John Elmerdahl Olsen. "Primer Design." In Introduction to Bioinformatics in Microbiology, 81–102. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-99280-8_5.
Full textLinhart, Chaim, and Ron Shamir. "Degenerate Primer Design." In PCR Primer Design, 220–44. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-528-2_11.
Full textHyndman, David L., and Masato Mitsuhashi. "PCR Primer Design." In PCR Protocols, 81–88. Totowa, NJ: Humana Press, 2003. http://dx.doi.org/10.1007/978-1-4612-0055-0_19.
Full textKaiser, Mark J., and Brian F. Snyder. "Jackup Design Primer." In The Offshore Drilling Industry and Rig Construction in the Gulf of Mexico, 165–83. London: Springer London, 2013. http://dx.doi.org/10.1007/978-1-4471-5152-4_10.
Full textShe, Jinjuan, Carolyn Conner Seepersad, Katja Holtta-Otto, and Erin F. MacDonald. "Priming Designers Leads to Prime Designs." In Understanding Innovation, 251–73. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-60967-6_13.
Full textBaumgardt, Michael. "Rahmen [Primes]." In Web Design kreativ!, 83–92. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-662-13416-0_5.
Full textLeister, Dario, and Claudio Varotto. "GST-PRIME." In PCR Primer Design, 141–57. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-528-2_7.
Full textConference papers on the topic "Primer designs"
Singh, Ratnesh, Na Li, Zunping Luo, Anna McGeachy, Antonio F. Martinez Alcantara, Aren Ewing, and Fiona C. Hyland. "Abstract 1207: Custom next-generation sequencing primer designs for targeted sequencing of multi strain viral targets." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-1207.
Full textTomašegović, Tamara, Sanja Mahović Poljaček, Tomislav Hudika, and Andrea Marče. "Preliminary report on properties and interaction of layers in “board-biodegradable primer-printing ink” screen-printed system." In 11th International Symposium on Graphic Engineering and Design. University of Novi Sad, Faculty of technical sciences, Department of graphic engineering and design, 2022. http://dx.doi.org/10.24867/grid-2022-p80.
Full textFitton, Daniel, and Janet C. Read. "Primed Design Activities." In NordiCHI '16: 9th Nordic Conference on Human-Computer Interaction. New York, NY, USA: ACM, 2016. http://dx.doi.org/10.1145/2971485.2971529.
Full textKIDIDIS, ANDREW. "Mil-Prime specification for parachutes." In Aerospace Design Conference. Reston, Virigina: American Institute of Aeronautics and Astronautics, 1993. http://dx.doi.org/10.2514/6.1993-1247.
Full textAmoozegar, Maryam, and Elham Rezvannejad. "Primer design using gravitational search algorithm." In 2014 Iranian Conference on Intelligent Systems (ICIS). IEEE, 2014. http://dx.doi.org/10.1109/iraniancis.2014.6802608.
Full textChamberlain, Paul, and Claire Craig. "A Design Primer for the Domestication of Health Technologies." In Design Research Society Conference 2016. Design Research Society, 2016. http://dx.doi.org/10.21606/drs.2016.431.
Full textKrupski, Tomasz, and Ewa Hermanowicz. "Novel designs of recursive discrete-time sinusoidal oscillators." In 2009 Ph.D. Research in Microelectronics and Electronics (PRIME). IEEE, 2009. http://dx.doi.org/10.1109/rme.2009.5201337.
Full textDeharbe, David, Pascal Fontaine, Daniel Le Berre, and Bertrand Mazure. "Computing prime implicants." In 2013 Formal Methods in Computer-Aided Design (FMCAD). IEEE, 2013. http://dx.doi.org/10.1109/fmcad.2013.6679390.
Full textRadojicic, Carna, and Christoph Grimm. "Formal verification of mixed-signal designs using extended affine arithmetic." In 2016 12th Conference on Ph.D. Research in Microelectronics and Electronics (PRIME). IEEE, 2016. http://dx.doi.org/10.1109/prime.2016.7519482.
Full textHauptmann, Joerg, Dario Giotta, and Ulrich Gaier. "Analog design trends in communication systems." In 2013 9th Conference on Ph.D. Research in Microelectronics and Electronics (PRIME). IEEE, 2013. http://dx.doi.org/10.1109/prime.2013.6603137.
Full textReports on the topic "Primer designs"
Lund, H. Gyde, and Charles E. Thomas. A primer on stand and forest inventory designs. Washington, DC: U.S. Department of Agriculture, Forest Service, 1989. http://dx.doi.org/10.2737/wo-gtr-54.
Full textChrist, Richard E., Joseph A. Conroy, and Aubry L. Briggs. Systematic Organizational Design (SORD) Methodology: A Primer. Fort Belvoir, VA: Defense Technical Information Center, September 1990. http://dx.doi.org/10.21236/ada230103.
Full textBartkowski, Peter T. Design of a Percussion and Electric Primer Gun Firing Power Supply. Fort Belvoir, VA: Defense Technical Information Center, July 2014. http://dx.doi.org/10.21236/ada607546.
Full textDutton, Todd, and Andrew Brant. Design of a Combined Ballistic Simulator and Primer Force Experimental Fixture. Fort Belvoir, VA: Defense Technical Information Center, August 2015. http://dx.doi.org/10.21236/ada623361.
Full textMCGREW, D. L. Project Design Concept Primary Ventilation System. Office of Scientific and Technical Information (OSTI), October 2000. http://dx.doi.org/10.2172/805372.
Full textTian, Shu. Primer on Social Bonds and Recent Developments in Asia. Asian Development Bank, February 2021. http://dx.doi.org/10.22617/spr210045-2.
Full textBao, Jieyi, Xiaoqiang Hu, Cheng Peng, Yi Jiang, Shuo Li, and Tommy Nantung. Truck Traffic and Load Spectra of Indiana Roadways for the Mechanistic-Empirical Pavement Design Guide. Purdue University, 2020. http://dx.doi.org/10.5703/1288284317227.
Full textChoi, Syngjoo, Lars Nesheim, and Imran Rasul. Reserve price effects in auctions: estimates from multiple RD designs. Institute for Fiscal Studies, October 2010. http://dx.doi.org/10.1920/wp.cem.2010.3010.
Full textGutierrez-Arias, Ruvistay, Dawid Pieper, Carole Lunny, Rodrigo Torres-Castro, Raúl Aguilera-Eguía, and Pamela Seron. Strategies used to manage overlap of primary study data by exercise-related overviews authors. Protocol for a systematic methodological review. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, May 2022. http://dx.doi.org/10.37766/inplasy2022.5.0161.
Full textDinerstein, Michael, Liran Einav, Jonathan Levin, and Neel Sundaresan. Consumer Price Search and Platform Design in Internet Commerce. Cambridge, MA: National Bureau of Economic Research, August 2014. http://dx.doi.org/10.3386/w20415.
Full text