Relevant bibliographies by topics / Primary Human Tumor Cultures

Academic literature on the topic 'Primary Human Tumor Cultures'

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Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Primary Human Tumor Cultures"

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Girinsky, T., J. M. Cosset, N. Chavaudra, F. Geara, R. Lubin, H. Bounik, E. Janot, et al. "Primary Cultures from Human Tumor Biopsies." International Journal of Radiation Biology 56, no. 5 (January 1989): 845–46. http://dx.doi.org/10.1080/09553008914552181.

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Zaffaroni, N., R. Villa, L. Oriandi, and R. Silvestrini. "Radiosensitivity testing of human tumor primary cultures." European Journal of Cancer 29 (January 1993): S217. http://dx.doi.org/10.1016/0959-8049(93)91839-d.

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Westphal, Manfred, Hildergard Nausch, and Hans-Dietrich Herrmann. "Cyst Fluids of Malignant Human Brain Tumors Contain Substances That Stimulate the Growth of Cultured Human Gliomas of Various Histological Type." Neurosurgery 25, no. 2 (August 1, 1989): 196–201. http://dx.doi.org/10.1227/00006123-198908000-00007.

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Abstract The contents of 14 cysts that were located within human intracranial tumors were obtained at surgery by needle aspiration. These tumor cyst fluids (TCFs) were mostly derived from glial tumors (10 cases). TCFs from one metastasis from a mammary carcinoma, one cystic meningioma, one hemangioblastoma, and a cystic acoustic neurinoma were also included. These TCFs were added to primary cultures of human gliomas, established human glioma cell lines, and normal human arachnoid cells in culture. The presence of proliferation-promoting factors in all cyst fluids could be demonstrated. On the basis of the response patterns of the cultures, it was possible to distinguish different levels of growth autonomy and growth factor sensitivity among these cultures and to speculate about varying degrees of cellular autocrine activation. The TCFs appear to contain factors that are not normally present in fetal calf serum, which is a regular constituent of most cell culture media. Some primary cultured cells as well as cell lines react in an oversensitive manner to the addition of TCFs.
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Dougherty, Mark, Eric Taylor, and Marlan Hansen. "TMET-34. RADIATION METABOLOMICS IN PRIMARY HUMAN MENINGIOMA AND SCHWANNOMA: EARLY EXPERIENCE AND INITIAL RESULTS." Neuro-Oncology 24, Supplement_7 (November 1, 2022): vii269. http://dx.doi.org/10.1093/neuonc/noac209.1039.

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Abstract Introduction Meningiomas and schwannomas account for 45% of primary CNS tumors. Yet when surgery and radiation fail, no further treatments exist. Metabolomics has been used to discover new cancer therapies; however, to date few have used metabolomics to study meningiomas and schwannomas. Here we present initial results and lessons learned from this novel endeavor. METHODS Primary tumors were obtained from patients during surgery and immediately taken for culturing or xenograft implantation. Upon reaching >90% confluence, cultures were treated with 0gy, 3gy, 10gy, or 20gy gamma radiation, then flash frozen 6 or 72 hours post-treatment. Xenograft tumors were implanted in nude mice. MRI 4 weeks post-implantation confirmed tumor viability. Mice were then given 10gy, 20gy, or sham radiation treatment. Xenografts were harvested 72 hours post-treatment. Metabolites were measured with a ThermoISQ gas chromatography-mass spectrometer. RESULTS Eleven meningiomas and nine schwannomas were successfully cultured. Unsupervised hierarchical clustering of cultures demonstrated greater influence from tumor of origin than from radiation. Univariate analysis of schwannoma xenografts demonstrated elevated ornithine following radiation (fold change 1.62; P = 0.008). However, principal component analysis did not show significant between-group differentiation. Orthotopic meningioma xenografts did not produce sufficient tissue for metabolomics; however, subsequent subcutaneous implants have been successful (data forthcoming). CONCLUSION Standard cell cultures did not reveal significant metabolic changes following radiation; it is unclear whether this was due to culture technique or inter-tumor heterogeneity. In radiated schwannoma xenografts, elevated ornithine may implicate related pathways such as ornithine decarboxylase-mediated polyamide synthesis for DNA double-strand break repair. Compared to other ‘-omics’ studies, metabolomics requires more tissue per sample ( >10mg) and is more sensitive to environmental conditions. Thus, large sample sizes are needed to detect significant changes, and xenografts are likely superior to cell culture. Future plans include increased xenograft sample size and stable isotope tracing for pathway analysis.
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Strietz, Juliane, Stella S. Stepputtis, Marie Follo, Peter Bronsert, Elmar Stickeler, and Jochen Maurer. "Human Primary Breast Cancer Stem Cells Are Characterized by Epithelial-Mesenchymal Plasticity." International Journal of Molecular Sciences 22, no. 4 (February 11, 2021): 1808. http://dx.doi.org/10.3390/ijms22041808.

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Triple-negative breast cancer (TNBC) is one of the most aggressive subtypes of breast cancer, with only limited treatment options available. Recently, cancer stem cells (CSCs) have emerged as the potential drivers of tumor progression due to their ability to both self-renew and give rise to differentiated progeny. The CSC state has been linked to the process of epithelial-mesenchymal transition (EMT) and to the highly flexible state of epithelial-mesenchymal plasticity (EMP). We aimed to establish primary breast cancer stem cell (BCSC) cultures isolated from TNBC specimens. These cells grow as tumor spheres under anchorage-independent culture conditions in vitro and reliably form tumors in mice when transplanted in limiting dilutions in vivo. The BCSC xenograft tumors phenocopy the original patient tumor in architecture and gene expression. Analysis of an EMT-related marker profile revealed the concomitant expression of epithelial and mesenchymal markers suggesting an EMP state for BCSCs of TNBC. Furthermore, BCSCs were susceptible to stimulation with the EMT inducer TGF-β1, resulting in upregulation of mesenchymal genes and enhanced migratory abilities. Overall, primary BCSC cultures are a promising model close to the patient that can be used both in vitro and in vivo to address questions of BCSC biology and evaluate new treatment options for TNBC.
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Muller, D., J. P. Fricker, R. Millon-Collard, J. Abecassis, J. Pusel, M. Eber, and G. Methlin. "Characterization of cell types in human breast tumor primary cultures." Biology of the Cell 61, no. 1-2 (1987): 91–99. http://dx.doi.org/10.1111/j.1768-322x.1987.tb00574.x.

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Arundel, Carla, Susan Bock, William A. Brock, and Philip J. Tofilon. "Radiosensitization of primary human tumor cell cultures by N-methylformamide." International Journal of Radiation Oncology*Biology*Physics 13, no. 5 (May 1987): 753–57. http://dx.doi.org/10.1016/0360-3016(87)90295-1.

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Le Rhun, E., C. von Achenbach, F. Sahm, S. S. Wang, M. C. Neidert, E. Rushing, T. Lawhon, H. Schneider, A. von Deimling, and M. Weller. "OS8.6 Sensitivity of human meningioma cells to the cyclin-dependent kinase inhibitor, TG02." Neuro-Oncology 21, Supplement_3 (August 2019): iii17. http://dx.doi.org/10.1093/neuonc/noz126.056.

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Abstract BACKGROUND Standards of care for meningioma include surgical resection, which may be curative, and radiotherapy as required. Pharmacotherapy plays only a minor role in this disease; however, novel systemic approaches are urgently needed for patients who are no longer candidates for local therapy. MATERIAL AND METHODS We generated primary cultures from surgically removed meningiomas to explore the activity of a novel cyclin-dependent kinase inhibitor, TG02, in meningioma cell cultures. Tumor and cell cultures were characterized by mutation profiling and DNA methylation profiling. DNA methylation data were used to allot each sample to one out of six previously established meningioma methylation classes: benign (ben)-1, 2, 3, intermediate (int)-A, B, and malignant (mal). The activity of TG02 was assessed by standard cell culture assays. RESULTS Cell cultures were derived from nine meningiomas. Four tumors assigned to the methylation class ben-2 showed the same class in the cell culture whereas cell cultures from five non-ben-2 tumors showed a different class, a more malignant class in four of five patients. Cell cultures were uniformly sensitive to the growth inhibitory effects of TG02 in the nanomolar range. Assignment of the cell cultures to a more malignant methylation classifier appeared to be more closely associated with TG02 sensitivity than assignment to a higher WHO grade of the primary tumors. CONCLUSION Primary cell cultures from meningioma facilitate the investigation of the anti-meningioma activity of novel agents. TG02, an orally available cyclin-dependent kinase inhibitor, warrants further exploration in this disease.
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Чернов, А. Н., Е. П. Баранцевич, Э. С. Галимова, and М. М. Галагудза. "Cell cultures of human malignant tumors in development of new anticancer therapies." Nauchno-prakticheskii zhurnal «Patogenez», no. 4() (January 30, 2018): 13–23. http://dx.doi.org/10.25557/gm.2018.4.9744.

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Современный эффективный скрининг новых противоопухолевых химиопрепаратов и биологических препаратов на доклиническом этапе невозможен без применения моделей культур опухолевых клеток. К таким моделям относят первичные культуры клеток и клеточные линии опухолей человека, культивируемые в двумерной (2D) и трехмерной (3D) системах. В обзоре обсуждаются различные аспекты применения моделей клеточных культур неоплазий человека, их актуальность в исследованиях противоопухолевой эффективности препаратов. Current effective preclinical screening of new anticancer chemotherapies and biological medicines requires cancer cell culture models. Such models include primary cell cultures and human tumor cell lines cultured in two-dimensional (2D) and three-dimensional (3D) systems. This review discussed different aspects of using human tumor cell culture models and their relevance for studying efficacy of antitumor drugs.
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Whigham, Amy, Brown Brandee, Avtandyl Kochiashvili, James L. Netterville, Brian B. Burkey, Robert J. Sinard, and Wendell G. Yarbrough. "Short-Term Culture and In-Vivo Modeling of Primary HNSCC." Otolaryngology–Head and Neck Surgery 139, no. 2_suppl (August 2008): P93—P94. http://dx.doi.org/10.1016/j.otohns.2008.05.502.

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Problem Head and neck squamous cell carcinoma (HNSCC) accounts for 4% of annual U.S. cancer deaths. In-vivo models exist using established HNSCC lines, but currently there is no such model that allows consistent growth of HNSCC from primary tumors. Methods Primary HNSCC tissue was obtained from 103 patients at biopsy/resection, disaggregated and seeded onto collagen-coated plates in keratinocyte growth media with 10% FBS, additives and antibiotics. After short-term growth in culture, cells were transferred to denuded rat tracheas and implanted subcutaneously in nude mice. Indirect immunofluorescent staining using antibodies specific to cytokeratin, vimentin and nuclear Ku was performed to determine cell lineage and origin. Results Cultured cells exhibited morphology consistent with epithelial or stromal derivation. 80% of cultures had viable cells present at 10 days and 24% were maintained 30 days or longer. 5 cultures (5%) proliferated after multiple passages and thrived on uncoated plates in the absence of mesenchymal cells. The xenograft model was able to successfully establish tumors in vivo from 59% of primary tumors. Immunostaining for nuclear Ku and cytokeratin confirmed human origin and epithelial cell lineage, respectively. Conclusion The high success rate indicates that selective growth and survival pressures for short-term culture of primary HNSCC may be considerably less than for establishment of cell lines. Additionally, the techniques permit tumor-derived epithelial and mesenchymal cells to be cultured simultaneously. Preliminary data for the in-vivo trachea xenograft model is promising. A luciferase lentiviral system has been created to allow monitoring of tumor growth in vivo with serial live animal imaging. Significance These short-term culture techniques may more accurately characterize both the biological diversity of HNSCC and tumor-stromal cell interactions. Once optimized, the trachea xenograft model can be used to determine the in-vivo response of a heterogeneous group of HNSCC to standard and novel therapies. Support Funds provided by an endowment for the Barry Baker Laboratory for Head and Neck Oncology, the Vanderbilt Ingram Cancer Center Endowed Professorship Fund, and the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation.
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Dissertations / Theses on the topic "Primary Human Tumor Cultures"

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Laryea, D., A. Isaksson, Colin W. Wright, R. Larsson, and P. Nygren. "Characterization of the cytotoxic activity of the indoloquinoline alkaloid cryptolepine in human tumour cell lines and primary cultures of tumour cells from patients." Springer, 2009. http://hdl.handle.net/10454/4533.

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The plant derived indoloquinoline alkaloid cryptolepine was investigated for its cytotoxic properties in 12 human tumour cell lines and in primary cultures of tumour cells from patients. The fluorometric microculture cytotoxicity assay was used to assess cytotoxicity and DNA mocro-array analysis to evaluate gene expression. Cryptolepine mean IC50 in the cell line panel was 0.9 microM compared with 1.0 and 2.8 microM in haemaotological and solid tumour malignancies respectively. Among patient solid tumour samples, those from breast cancer were the most sensitive and essentially as sensitive as haematological malignancies, respectively. Among patient solid tumour samples, those from breast cancer were the most sensitive and essentially as senstive as haematological malignancies. Cryptolepine activity showed highest correlations to topoisomerase II and microtubule targetting drugs. In the cell lines cryptolepine activity was essentially unaffected by established mechanisms of drug resistance. A number of genes were identified as associated with cryptolepine activity. In conclusion, cryptolepine shows interesting in vitro cytotoxic properties and its further evaluation as an anticancer drug seems warranted.
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Cunha, Virgínia Filipa Pereira Monteiro da. "Human adipose tissue primary cultures and impact in prostate cancer." Master's thesis, Universidade de Aveiro, 2009. http://hdl.handle.net/10773/8985.

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Mestrado em Biologia Aplicada - Molecular e Celular
Prostate cancer (Pca) is one of the most frequent diagnosed neoplasies and the second cause of cancer-related death in the world, in men. Between others risk factors, obesity has been associated to Pca although the innerent mechanisms to this association remain to be clear. With this work, throuhg in vitro studies, we wish to contribute to the understanding of the impact of white adipose tissue and its sub-fractions (adipocytes and stromal vascular fraction), from visceral and periprostatic anatomic regions, in celular proliferation, apoptosis and invasion of castration sensitivity (LNCaP) and castration resistant (PC-3) prostate cells. With the purpose of obtaining answers directly from humam studies, were performed visceral and periprostatic adipose tissue primary cultures obtained during urologic surgeries (radical prostatectomy and prostatic adenomectomy) (n=16). Adipose tissue was used to make primary organotipical cultures (WAT) and after collagenase digestion adipocytes and SVF primary cultures. Sobrenatants and infranatants of each culture were collect and used as conditioned medium representing adipokines production. LNCaP and PC-3 cell lines were stimulated with these mediums and apoptosis, proliferation and invasion were evaluated, in vitro. This study model represent a potential form for analyze the impact of adipose tissue in tumor cells, allowing to evaluate adipose tissue-tumor interactions. The results show that adipose tissue promotes tumor cells proliferation, that periprostatic adipose tissue increase apoptosis in obese individuals and that SVF subfraction suppresses invasion of PC-3 cells through a direct effect in tumor cells.
O Cancro da próstata (CaP) é uma das neoplasias mais frequentemente diagnosticadas e a segunda causa de morte por cancro no mundo nos homens. Entre outros factores de risco, a obesidade tem sido frequentemente associada a CaP, embora permaneçam por esclarecer os mecanismos subjacentes a esta associação. Com o presente trabalho pretendeu-se através de estudos in vitro, contribuir para a compreensão do impacto do tecido adiposo branco e suas sub-fracções (adipócitos e fracção vascular estromal), com origens anatómicas periprostática e viceral, na proliferação, apoptose, e invasão celular de células de cancro da próstata sensíveis à castração (LNCaP) e resistentes à castração (PC-3). Com o propósito de obter respostas directamente através de estudos em humanos, foram efectuadas culturas primárias de tecido adiposo periprostático e visceral obtido durante cirurgias urológicas (prostatectomia radical e adenomectomia prostática) (n=16). O tecido adiposo foi utilizado para realizar culturas primárias organotípicas (tecido adiposo total fraccionado) e após digestão com colagenase culturas primárias de adipócitos e de células da fracção vascular estromal do tecido adiposo. Foram colhidos sobrenadantes e infranadantes destas culturas de tecido adiposo e utilizados como meios condicionados representativos da produção de adipocinas. As linhas celulares LNCaP e PC-3 foram estimuladas com estes meios e avaliados a apoptose, proliferação celular e invasividade tumoral in vitro. Este modelo de estudo representa um potencial meio para análise do impacto do tecido adiposo nas células tumorais, permitindo avaliar as interacções tecido adiposo-tumor. Os resultados evidenciam que o tecido adiposo promove a proliferação das células tumorais, que o tecido adiposo periprostático aumenta a apoptose em indivíduos obesos e que os SVF suprimem a invasão das PC-3 através de um efeito directo nas células tumorais.
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Ward, Rachel Ward. "Development of primary human keratinocyte cultures for studying chemical-induced skin irritation." Thesis, University of Nottingham, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241148.

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Al-Khalili, Lubna. "Gene regulation, intracellular signaling and membrane traffic : studies in primary human skeletal muscle cultures /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-866-1/.

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Johnson, Sara M. "Respiratory Syncytial Virus Uses CX3CR1 as a Cellular Receptor on Primary Human Airway Epithelial Cultures." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1448285474.

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Iskander, Sammy. "Characterisation [i.e. characterization] of HIV-1 glycoprotein 120 neurotoxicity in primary human serum-free CNS cultures." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0021/MQ58045.pdf.

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Broadbent, Lindsay Jane. "Exploitation of well-differentiated primary paediatric airway epithelial cell cultures (WD-PAECs) to study RSV/human host interactions." Thesis, Queen's University Belfast, 2016. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.695259.

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Respiratory syncytial virus (RSV) is the primary cause of severe lower respiratory tract infections in children. We exploited well-differentiated primary paediatric airway epithelial cells (WD-PAECs) to investigate innate immune responses to RSV. We demonstrated that RSV-conditioned medium (CMRSV) treated WD-PAECs were highly resistant to subsequent RSV or Sendai virus infection. IL-29 significantly contributes to the antiviral activity of CMRSV. Apical pre-treatment, but not basolateral pre-treatment, of WD-PBECs with IL-29 decreased RSV growth kinetics. We found that IL28RA, a component of the type III IFN receptor, was located exclusively on the apical surface of WD-PBECs. We demonstrated that type IIllFNs modulate the innate immune responses to RSV infection. Pre-treatment of WD-PBECs with a TLR4 signalling inhibitor resulted in massive reductions in RSV growth kinetics, and IL-29 and pro-inflammatory cytokine secretion. To elucidate the role of TLR4 in RSV infection we used inhibitors of TLR4 pathway intermediates. Our results showed it is likely that RSV-induced IL-29 is mediated, in part, by TLR4 signalling through NF-KB and/or p38 MAPK. We identified ten genes that were significantly upregulated in RSV-infected WD-PAEC cultures. Furthermore we identified nine genes that are differentially expressed between two patient cohorts: history of mild or severe RSV disease. The proteins encoded by isg15, rsad2 and tnfsf13b, were assessed for direct antiviral properties. However, they failed to reduce viral replication. The gene ptn was found to be differentially expressed in WD-PNECs from the two cohorts irrespective of RSV infection. Pre-treatment of WD-PBECs at 40C with PTN significantly reduced RSV replication. In summary, we demonstrated that RSV induced antiviral responses are mediated, in part, by IL-29. TLR4 signalling plays a significant role in RSV replication and induction of IL-29 and pro-inflammatory chemokines. We identified three potential anti-RSV molecules: IL-29, CLI-095 and PTN, which may be beneficial in the battle against RSV.
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Andersson, Maria. "Chemically Induced DNA Damage in Extended-term Cultures of Human Lymphocytes." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7179.

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Wong, Hing-ki Charmaine. "Viral determinants of influenza A (H5N1) associated TNF-a hyper-induction in human primary monocyte-derived macrophages." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B37659029.

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Wong, Hing-ki Charmaine, and 黃馨琦. "Viral determinants of influenza A (H5N1) associated TNF-a hyper-induction in human primary monocyte-derived macrophages." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37659029.

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Books on the topic "Primary Human Tumor Cultures"

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W, Masters John R., ed. Human cancer in primary culture: A handbook. Dordrecht: Kluwer Academic Publishers, 1991.

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Sherman, Mark E., Melissa A. Troester, Katherine A. Hoadley, and William F. Anderson. Morphological and Molecular Classification of Human Cancer. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780190238667.003.0003.

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Accurate and reproducible classification of tumors is essential for clinical management, cancer surveillance, and studies of pathogenesis and etiology. Tumor classification has historically been based on the primary anatomic site or organ in which the tumor occurs and on its morphologic and histologic phenotype. While pathologic criteria are useful in predicting the average behavior of a group of tumors, histopathology alone cannot accurately predict the prognosis and treatment response of individual cancers. Traditional measures such as tumor stage and grade do not take into account molecular events that influence tumor aggressiveness or changes in the tumor composition during treatment. This chapter provides a primer on approaches that use pathology and molecular biology to classify and subclassify cancers. It describes the features of carcinomas, sarcomas, and malignant neoplasms of the immune system and blood, as well as various high-throughput genomic platforms that characterize the molecular profile of tumors.
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Ayala, Francisco J., and Camilo J. Cela-Conde. Processes in Human Evolution. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780198739906.001.0001.

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Processes in Human Evolution is a fully reconsidered treatment of Human Evolution, the previous book by the same authors published by OUP in 2007. The major discoveries of the last decade have necessitated a completely revised view of how to understand human evolution, incorporating the advances in genetics, paleontology, ecology, archaeology, geography, and climate science. The genera Sahelanthropus, Ardipithecus, Orrorin, Australopithecus, Paranthropus, and Homo are analyzed in detail. Particular attention is given to the population migrations necessary to understand the origin and dispersion of the different genera and species in each continent. It becomes apparent that some of the accepted species may be just isolated populations and that the distinctive features of Homo sapiens may have been shared by other ancestors. The evolutionary process takes into account the features of the lithic cultures and their impact on the evolution of the cognitive capacities. The outcome is a new model about the evolution of symbolism. Processes in Human Evolution is intended as a primary textbook for university courses on human evolution. It may be used, as well, as supplementary reading in advanced undergraduate and graduate courses. It is also eminently suited for general-interest readers seeking a readable but up-to-date and fairly inclusive treatment of human origins and evolution.
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Hogh-Olesen, Henrik. The First Humans and the First Art. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780190927929.003.0003.

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The chapter follows the aesthetic impulse back to human prehistory and looks at prehistoric art. In order to establish our aesthetic inclinations as a primary impulse—and not just as a surplus phenomenon appearing in high cultures in times of plenty, when people have no better things to do—it is important to track this impulse back to its first expression and to the material living conditions at the time. Regarding the origin of this primary impulse, it is interesting to consider who these people were in terms of psychology. Who were the creators of the prehistoric art, and what motivated this activity?
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Kiyange, Fatia. Volunteering in hospice and palliative care in Africa. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198788270.003.0011.

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Volunteering in hospice and palliative care in Africa occurs in existing cultures of care for the sick by families and their communities. The high burden of Human immunodeficiency virus (HIV) in sub-Saharan Africa further accelerated the development and involvement of volunteers in the delivery of hospice and palliative care services, as well as other health services. Similar to the Alma Ata Declaration which recognizes the importance of community members in the provision of primary health care, the World Health Assembly resolution on palliative care recognizes the role of volunteers in services. Volunteers are integral to palliative care teams and can help address the human resources crisis in health care systems in Africa. Various models for volunteering have emerged, each with unique characteristics. Different terminologies are also used to describe volunteering in hospice and palliative care, although the roles of volunteers are very similar.
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Book chapters on the topic "Primary Human Tumor Cultures"

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Speirs, Valerie. "Primary Culture of Human Mammary Tumor Cells." In Culture of Human Tumor Cells, 205–19. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2005. http://dx.doi.org/10.1002/0471722782.ch9.

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Seidel, Sascha, Boyan K. Garvalov, and Till Acker. "Isolation and Culture of Primary Glioblastoma Cells from Human Tumor Specimens." In Methods in Molecular Biology, 263–75. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1785-3_19.

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Patel, Ambrish J., Mary D. Weir, John L. Darling, David G. T. Thomas, and Antony Hunt. "Biochemical studies of primary cultures from human astrocytomas: observations on cell growth and regulation of glutamine synthetase." In Biology of Brain Tumour, 15–25. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4613-2297-9_3.

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Vu, Binh Thanh, Hanh Thi Le, Nhan Lu-Chinh Phan, and Phuc Van Pham. "Optimization of Culture Medium for the Isolation and Propagation of Human Breast Cancer Cells from Primary Tumor Biopsies." In 6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6), 903–8. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-4361-1_153.

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Dillon, Jane P., Victoria J. Waring-Green, Adam M. Taylor, Peter J. M. Wilson, Mark Birch, Alison Gartland, and James A. Gallagher. "Primary Human Osteoblast Cultures." In Methods in Molecular Biology, 3–18. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-415-5_1.

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Wechsler, Wolfgang, and Guido Reifenberger. "Application of Immunohistochemistry for Tumor Grading in Human Neuro-Oncology." In Primary Brain Tumors, 133–42. New York, NY: Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3676-4_10.

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Sugawa, Noriaki, Yoshio Nakagawa, and Satoshi Ueda. "Point Mutations of Epidermal Growth Factor Receptor Transcripts in Primary Human Malignant Gliomas." In Brain Tumor, 233–36. Tokyo: Springer Japan, 1996. http://dx.doi.org/10.1007/978-4-431-66887-9_23.

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Fu, Vivian X., Steven R. Schwarze, Catherine A. Reznikoff, and David F. Jarrard. "The Establishment and Characterization of Bladder Cancer Cultures In Vitro." In Culture of Human Tumor Cells, 97–123. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2005. http://dx.doi.org/10.1002/0471722782.ch5.

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Frenster, Joshua D., Julio Inocencio, and Dimitris G. Placantonakis. "Lentiviral Transduction of Primary Human Glioblastoma Cultures." In Methods in Molecular Biology, 81–89. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7659-1_6.

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Welch, Danny R., and Douglas R. Hurst. "Beyond the Primary Tumor: Progression, Invasion, and Metastasis." In The Molecular Basis of Human Cancer, 203–16. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-59745-458-2_12.

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Conference papers on the topic "Primary Human Tumor Cultures"

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Nagourney, Robert Alan, Paula J. Bernard, Federico Francisco, and Steven S. Evans. "Abstract 4245: A comparative analysis of PI3K inhibitors in human tumor primary culture microspheroids." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-4245.

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Guerrero-Cazares, Hugo, Vivian Capilla-Gonzalez, Emily A. Lavell, Alejandro Ruiz-Valls, Linda Chen, Gabrielle Drummond, Sural Ranamukhaarachchi, and Alfredo Quinones-Hinojosa. "Abstract 3153: Slit-2 directs the migration of primary cultured human GBM tumor initiating cells." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-3153.

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Neal, James T., Michael Cantrell, Paul Rack, and Calvin J. Kuo. "Abstract LB-34: 3-Dimensional air-liquid interface organoid culture of primary human tumor biopsies." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-lb-34.

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Nagourney, Robert Alan, Paula J. Bernard, Federico R. Francisco, and Steven S. Evans. "Abstract 1764: Horizontal and vertical signal pathway inhibition in human tumor primary culture micro-spheroids." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-1764.

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Thielker, J., D. Freitag, U. Tiller, S. Schlieper, S. Grube, J. Walter, and O. Guntinas-Lichius. "Cultivation of primary cell cultures and isolation of stem cell-like cells from benign human salivary gland tumors." In Abstract- und Posterband – 91. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn – Welche Qualität macht den Unterschied. © Georg Thieme Verlag KG, 2020. http://dx.doi.org/10.1055/s-0040-1711449.

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Nagourney, Robert Alan, Paula J. Bernard, Eric Federico, Sophie Nguyen, and Steven S. Evans. "Abstract 3525: Functional analysis of epidermal growth factor receptor tyrosine kinase inhibitors: A comparison of Afatanib, Lapatinib, and Gefitinib in human tumor primary cultures." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-3525.

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Nagourney, Robert Alan, Paula J. Bernard, Federico Francisco, Meghan Cule, Ryan Wexler, and Steven S. Evans. "Abstract 2418: A comparison of NVP-AUY-922 (AUY) and Geldenamycin (G) in human tumor primary culture microspheroids." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-2418.

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Willnow, Lena, Stefan Tomiuk, Jutta Kollet, Stefan Wild, Silvia Rüberg, Claudius Fridrich, Peter Mallmann, et al. "Abstract 174: Isolation of primary human tumor cells significantly reduces bias in molecular analysis and improves culture of target cells." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-174.

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Sazinsky, Steve, Ani Nguyen, Mohammad Zafari, Ryan Phennicie, Joe Wahle, Veronica Komoroski, Kathryn Rooney, et al. "Abstract P105: Targeting VSIG4, a novel macrophage checkpoint, repolarizes suppressive macrophages which induces an inflammatory response in primary cell in vitro assays and fresh human tumor cultures." In Abstracts: AACR-NCI-EORTC Virtual International Conference on Molecular Targets and Cancer Therapeutics; October 7-10, 2021. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1535-7163.targ-21-p105.

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Nagourney, Robert Alan, Paula J. Bernard, Federico R. Francisco, and Steven S. Evans. "Abstract 2856: Functional profile of BEZ-235 activity in human tumor primary culture microspheroids byex vivoanalysis of programmed cell death (EVA/PCD)." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-2856.

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Reports on the topic "Primary Human Tumor Cultures"

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Peehl, Donna M. Development of Methodology to Maintain Primary Cultures of Normal and Malignant Human Prostatic Epithelial Cells In Vivo. Fort Belvoir, VA: Defense Technical Information Center, February 2008. http://dx.doi.org/10.21236/ada484336.

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Peehl, Donna M. Development of Methodology to Maintain Primary Cultures of Normal and Malignant Human Prostatic Epithelial Cell In Vivo. Fort Belvoir, VA: Defense Technical Information Center, February 2005. http://dx.doi.org/10.21236/ada434013.

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Splitter, Gary A., Menachem Banai, and Jerome S. Harms. Brucella second messenger coordinates stages of infection. United States Department of Agriculture, January 2011. http://dx.doi.org/10.32747/2011.7699864.bard.

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Aim 1: To determine levels of this second messenger in: a) B. melitensiscyclic-dimericguanosinemonophosphate-regulating mutants (BMEI1448, BMEI1453, and BMEI1520), and b) B. melitensis16M (wild type) and mutant infections of macrophages and immune competent mice. (US lab primary) Aim 2: To determine proteomic differences between Brucelladeletion mutants BMEI1453 (high cyclic-dimericguanosinemonophosphate, chronic persistent state) and BMEI1520 (low cyclicdimericguanosinemonophosphate, acute virulent state) compared to wild type B. melitensisto identify the role of this second messenger in establishing the two polar states of brucellosis. (US lab primary with synergistic assistance from the Israel lab Aim 3: Determine the level of Brucellacyclic-dimericguanosinemonophosphate and transcriptional expression from naturally infected placenta. (Israel lab primary with synergistic assistance from the US lab). B. Background Brucellaspecies are Gram-negative, facultative intracellular bacterial pathogens that cause brucellosis, the most prevalent zoonosis worldwide. Brucellosis is characterized by increased abortion, weak offspring, and decreased milk production in animals. Humans are infected with Brucellaby consuming contaminated milk products or via inhalation of aerosolized bacteria from occupational hazards. Chronic human infections can result in complications such as liver damage, orchitis, endocarditis, and arthritis. Brucellaspp. have the ability to infect both professional and non-professional phagocytes. Because of this, Brucellaencounter varied environments both throughout the body and within a cell and must adapt accordingly. To date, few virulence factors have been identified in B. melitensisand even less is known about how these virulence factors are regulated. Subsequently, little is known about how Brucellaadapt to its rapidly changing environments, and how it alternates between acute and chronic virulence. Our studies suggest that decreased concentrations of cyclic dimericguanosinemonophosphate (c-di-GMP) lead to an acute virulent state and increased concentrations of c-di-GMP lead to persistent, chronic state of B. melitensisin a mouse model of infection. We hypothesize that B. melitensisuses c-di-GMP to transition from the chronic state of an infected host to the acute, virulent stage of infection in the placenta where the bacteria prepare to infect a new host. Studies on environmental pathogens such as Vibrio choleraeand Pseudomonas aeruginosasupport a mechanism where changes in c-di-GMP levels cause the bacterium to alternate between virulent and chronic states. Little work exists on understanding the role of c-di-GMP in dangerous intracellular pathogens, like Brucellathat is a frequent pathogen in Israeli domestic animals and U.S. elk and bison. Brucellamust carefully regulate virulence factors during infection of a host to ensure proper expression at appropriate times in response to host cues. Recently, the novel secondary signaling molecule c-di-GMP has been identified as a major component of bacterial regulation and we have identified c-di-GMP as an important signaling factor in B. melitensishost adaptation. C. Major conclusions, solutions, achievements 1. The B. melitensis1453 deletion mutant has increased c-di-GMP, while the 1520 deletion mutant has decreased c-di-GMP. 2. Both mutants grow similarly in in vitro cultures; however, the 1453 mutant has a microcolony phenotype both in vitro and in vivo 3. The 1453 mutant has increased crystal violet staining suggesting biofilm formation. 4. Scanning electron microscopy revealed an abnormal coccus appearance with in increased cell area. 5. Proteomic analysis revealed the 1453 mutant possessed increased production of proteins involved in cell wall processes, cell division, and the Type IV secretion system, and a decrease in proteins involved in amino acid transport/metabolism, carbohydrate metabolism, fatty acid production, and iron acquisition suggesting less preparedness for intracellular survival. 6. RNAseq analysis of bone marrow derived macrophages infected with the mutants revealed the host immune response is greatly reduced with the 1453 mutant infection. These findings support that microlocalization of proteins involved in c-di-GMP homeostasis serve a second messenger to B. melitensisregulating functions of the bacteria during infection of the host.
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