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1

Takahashi, N., and T. Yamada. "Glucose metabolism by Prevotella intermedia and Prevotella nigrescens." Oral Microbiology and Immunology 15, no. 3 (June 2000): 188–95. http://dx.doi.org/10.1034/j.1399-302x.2000.150307.x.

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FRANDSEN, E. V. G., K. POULSEN, and M. KILIAN. "Confirmation of the Species Prevotella intermedia and Prevotella nigrescens." International Journal of Systematic Bacteriology 45, no. 3 (July 1, 1995): 429–35. http://dx.doi.org/10.1099/00207713-45-3-429.

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3

Kalfas, S., Z. Tigyi, M. Wikström, and A. S. Naidu. "Laminin binding to Prevotella intermedia." Oral Microbiology and Immunology 7, no. 4 (August 1992): 235–39. http://dx.doi.org/10.1111/j.1399-302x.1992.tb00031.x.

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4

Leung, K. P., H. Fukushima, W. E. Nesbitt, and W. B. Clark. "Prevotella intermedia fimbriae mediate hemagglutination." Oral Microbiology and Immunology 11, no. 1 (February 1996): 42–50. http://dx.doi.org/10.1111/j.1399-302x.1996.tb00335.x.

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5

Anumala, Deepa, Mohan Kumar Pasupuleti, and Ravindra Reddy Nagireddy. "Detection and Characterization of Prevotella Intermedia and Its In Vitro Susceptibility to Selected Antimicrobial Agents in Chronic Periodontitis and Acute Myocardial Infarction." December 2019 3, no. 1 (February 22, 2019): 1–6. http://dx.doi.org/10.26810/perioj.2019.a1.

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Background: Periodontal disease has been reported to play a causative role in acute myocardial infarction (AMI), which may add to the various risk factors associated with coronary heart disease. The objective of the present study was to investigate the presence of Prevotella intermedia – an established periodontal pathogen – in subgingival plaque samples of chronic periodontitis and AMI patients in order to identify a possible association, and to evaluate the susceptibility of Prevotella intermedia to nine antimicrobial agents. Methods: After undergoing screening for eligibility, a total of 50 subjects were included in the present study. Twenty patients were diagnosed with AMI and generalized chronic periodontitis (Group I), 20 patients were diagnosed with only AMI (Group II), and 10 subjects were healthy controls (Group III). The isolated Prevotella intermedia strains were tested for susceptibility to bacitracin, chloramphenicol, penicillin G, polymyxin, gentamycin, neomycin, tetracycline, cefotaxime, and cefoxitin using an antibiotic zonescale to determine minimum inhibitory concentrations (MICs). Results: Periodontal pathogens were identified by phenotypic and enzymatic methods. The mean bacterial load of Prevotella intermedia species was higher in Group I compared to Group II and Group III. It was also found that pencillin G, gentamycin, neomycin, tetracycline, cefotaxime, and cefoxitin inhibited 90% of Prevotella intermedia, whereas bacitracin, chloramphenicol, and polymyxin inhibited 80% of Prevotella intermedia. Thus, only 10% of Prevotella intermedia were resistant to these antibiotics. Conclusion: The present study confirms that Prevotella intermedia is associated with chronic periodontitis and AMI.
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Mättö, Jaana, Sirkka Asikainen, Marja-Liisa Väisänen, Birgitta Von Troil-Lindén, Eija Könönen, Maria Saarela, Kari Salminen, Sydney M. Finegold, and Hannele Jousimies-Somer. "β-Lactamase Production in Prevotella intermedia,Prevotella nigrescens, and Prevotella pallensGenotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents." Antimicrobial Agents and Chemotherapy 43, no. 10 (October 1, 1999): 2383–88. http://dx.doi.org/10.1128/aac.43.10.2383.

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ABSTRACT The present study investigated the β-lactamase production of 73Prevotella intermedia, 84 Prevotella nigrescens, and 14 Prevotella pallens isolates and their in vitro susceptibilities to six antimicrobial agents. TheP. intermedia and P. nigrescens isolates were recovered from oral and extraoral samples obtained from subjects in two geographic locations from 1985 to 1995. The clonality of the β-lactamase-positive and β-lactamase-negative isolates and the clustering of the genotypes were studied by arbitrarily primed-PCR fingerprinting. β-Lactamase production was detected in 29% ofP. intermedia isolates, 29% of P. nigrescensisolates, and 57% of P. pallens isolates. No difference in the frequencies of β-lactamase production by P. intermedia and P. nigrescens between isolates from oral and extraoral sites, between isolates obtained at different time periods, or between P. intermedia isolates from different geographic locations was observed. However, the P. nigrescens isolates from the United States were significantly more frequently (P = 0.015) β-lactamase positive than those from Finland. No association between the genotypes and β-lactamase production or between the genotypes and the sources of the isolates was found. The penicillin G MICs at which 90% of the isolates were inhibited were 8 μg/ml for P. intermedia, 8 μg/ml for P. nigrescens, and 16 μg/ml for P. pallens. For the β-lactamase-negative isolates, the corresponding values were 0.031, 0.031, and 0.125 μg/ml, and for the β-lactamase-positive isolates, the corresponding values were 16, 8, and 32 μg/ml. All isolates were susceptible to amoxicillin-clavulanate, cefoxitin, metronidazole, azithromycin, and trovafloxacin. The MICs of amoxicillin-clavulanate and cefoxitin were relatively higher for the β-lactamase-positive population than for the β-lactamase-negative population.
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7

Dahlén, G. "Prevotella intermedia and Prevotella nigrescens serotypes, ribotypes and binding characteristics." FEMS Microbiology Letters 138, no. 1 (April 15, 1996): 89–95. http://dx.doi.org/10.1016/0378-1097(96)00095-x.

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8

Luong, N., J. Tsai, and C. Chen. "Susceptibilities of Eikenella corrodens,Prevotella intermedia, and Prevotella nigrescensClinical Isolates to Amoxicillin and Tetracycline." Antimicrobial Agents and Chemotherapy 45, no. 11 (November 1, 2001): 3253–55. http://dx.doi.org/10.1128/aac.45.11.3253-3255.2001.

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ABSTRACT The AB Biodisk Etest showed that 106 (100%) and 98 (92%) isolates of Eikenella corrodens were susceptible to amoxicillin and tetracycline, respectively. Twenty-three (68%) Prevotella intermedia isolates and 14 (67%) Prevotella nigrescens isolates were susceptible to amoxicillin. Seventy-nine percent of the P. intermedia isolates and 67% of theP. nigrescens isolates were susceptible to tetracycline. A higher percentage of β-lactamase-producing isolates of P. intermedia and P. nigrescens were identified with selective agar containing amoxicillin than with nonselective agar.
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9

Tanaka, Shoji, Mikako Yoshida, Yukio Murakami, Takako Ogiwara, Masao Shoji, Satoko Kobayashi, Sigeru Watanabe, Mamoru Machino, and Seiichiro Fujisawa. "The Relationship of Prevotella intermedia, Prevotella nigrescensand Prevotella melaninogenica in the Supragingival Plaque of Children, Caries and Oral Malodor." Journal of Clinical Pediatric Dentistry 32, no. 3 (April 1, 2008): 195–200. http://dx.doi.org/10.17796/jcpd.32.3.vp657177815618l1.

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Purpose: A relationship between the distribution of periodontal bacteria species and malodor in children has not been sufficiently investigated. The present study was undertaken to determine the presence of 3 periodontopathic bacteria (Prevotella spp. P. intermedia, P. nigrescens, P. melaninogenica) in the supragingival plaques of 3 to 16-year-old children with different oral health conditions and oral malodor. Methods: The number of decayed and filled primary teeth (df) and Decayed, Missing and Filled permanent teeth (DMF),Papillary Marginal and Attached gingivitis (PMA) index, Oral Hygiene Index (OHI), and oral malodor of each subject were determined prior to the collection of supragingival plaques. Three periodontopathic bacteria(P. intermedia, P. nigrescens, P. melaninogenica ) in supragingival plaques were detected by using an immunoslot blot assay with monoclonal antibodies specific for each microorganism. Findings: The frequencies of periodontopathic bacteria in children with and without caries were not significantly different from each other. Positivity for P. intermedia, but not for P. nigrescens or P. melaninogenica was correlated with oral malodor. Oral malodor was also correlated with the debris index, a component of OHI. The group with the higher OHI showed a higher prevalence of periodontopathic bacteria. For the 3 periodontopathic bacteria in the subjects tested, plaques positive for any of them were not age related. However,the frequencies of all 3 periodontopathic bacteria were the highest in the 3-6-year olds. Conclusion: The supragingival plaques in children can harbor 3 species of periodontopathic bacteria, P. intermedia,P. nigrescens, and P. melaninogenica.
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10

Stubbs, S., MAO Lewis, RJ Waddington, and G. Embery. "Hydrolytic and depolymerising enzyme activity of Prevotella intermedia and Prevotella nigrescens." Oral Diseases 2, no. 4 (June 28, 2008): 272–78. http://dx.doi.org/10.1111/j.1601-0825.1996.tb00237.x.

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11

Takahashi, N., and T. Yamada. "Pathways for amino acid metabolism by Prevotella intermedia and Prevotella nigrescens." Oral Microbiology and Immunology 15, no. 2 (April 2000): 96–102. http://dx.doi.org/10.1034/j.1399-302x.2000.150205.x.

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12

Kalfas, Sotirios, Mats Andersson, Stig Edwardsson, Arne Forsgren, and A. Satyanarayan Naidu. "Human lactoferrin binding to Porphyromonas gingivalis, Prevotella intermedia and Prevotella melaninogenica." Oral Microbiology and Immunology 6, no. 6 (December 1991): 350–55. http://dx.doi.org/10.1111/j.1399-302x.1991.tb00506.x.

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13

Mättö, J., M. Saarela, B. Troil-Lindén, E. Könönen, H. Jousimies-Somer, H. Torkko, S. Alaluusua, and S. Asikainen. "Distribution and genetic analysis of oral Prevotella intermedia and Prevotella nigrescens." Oral Microbiology and Immunology 11, no. 2 (April 1996): 96–102. http://dx.doi.org/10.1111/j.1399-302x.1996.tb00342.x.

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14

Conrads, G., K. Pelz, B. Hughes, I. Seyfarth, and D. A. Devine. "Optimized oligonucleotides for the differentiation of Prevotella intermedia and Prevotella nigrescens." Oral Microbiology and Immunology 12, no. 2 (April 1997): 117–20. http://dx.doi.org/10.1111/j.1399-302x.1997.tb00627.x.

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15

Steenbergen, T. J. M., C. J. Bosch-Tijhof, M. D. A. Petit, and U. Velden. "Intra-familial transmission and distribution of Prevotella intermedia and Prevotella nigrescens." Journal of Periodontal Research 32, no. 4 (May 1997): 345–50. http://dx.doi.org/10.1111/j.1600-0765.1997.tb00543.x.

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16

Cookson, Adrian L., Pauline S. Handley, Alan E. Jacob, G. Keith Watson, and Clive Allison. "Coaggregation between Prevotella nigrescens and Prevotella intermedia with Actinomyces naeslundii strains." FEMS Microbiology Letters 132, no. 3 (October 1995): 291–96. http://dx.doi.org/10.1111/j.1574-6968.1995.tb07848.x.

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17

Sakamoto, Mitsuo, Natsuko Suzuki, and Masaaki Okamoto. "Prevotella aurantiaca sp. nov., isolated from the human oral cavity." International Journal of Systematic and Evolutionary Microbiology 60, no. 3 (March 1, 2010): 500–503. http://dx.doi.org/10.1099/ijs.0.012831-0.

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Two anaerobic, pigmented, non-spore-forming, Gram-stain-negative, rod-shaped strains isolated from the human oral cavity, OMA31T and OMA130, were characterized by determining their phenotypic and biochemical features, cellular fatty acid profiles and phylogenetic positions based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that the new isolates belonged to a single species of the genus Prevotella. The two isolates showed 100 % 16S rRNA gene sequence similarity with each other and were most closely related to Prevotella intermedia ATCC 25611T with 96.4 % 16S rRNA gene sequence similarity; the next most closely related strains to the isolates were Prevotella pallens AHN 10371T (96.1 %) and Prevotella falsenii JCM 15124T (95.3 %). Phenotypic and biochemical characteristics of the isolates were the same as those of P. intermedia JCM 12248T, P. falsenii JCM 15124T and Prevotella nigrescens JCM 12250T. The isolates could be differentiated from P. pallens JCM 11140T by mannose fermentation and α-fucosidase activity. Conventional biochemical tests were unable to differentiate the new isolates from P. intermedia, P. falsenii and P. nigrescens. However, hsp60 gene sequence analysis suggested that strain OMA31T was not a representative of P. intermedia, P. pallens, P. falsenii or P. nigrescens. Based on these data, a novel species of the genus Prevotella, Prevotella aurantiaca sp. nov., is proposed, with OMA31T (=JCM 15754T=CCUG 57723T) as the type strain.
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18

Kim, Sung-Jo. "Chemical and Immunobiological Characterization of Lipopolysaccharides from Prevotella intermedia and Prevotella nigrescens." Journal of the Korean Academy of Periodontology 34, no. 2 (2004): 461. http://dx.doi.org/10.5051/jkape.2004.34.2.461.

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19

Bae, Kwang-Shik, J. Craig Baumgartner, Thomas R. Shearer, and Larry L. David. "Occurrence of Prevotella nigrescens and Prevotella intermedia in infections of endodontic origin." Journal of Endodontics 23, no. 10 (October 1997): 620–23. http://dx.doi.org/10.1016/s0099-2399(97)80173-2.

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20

Jansen, H. J., D. Grenier, and J. S. Hoeven. "Characterization of immunoglobulin G-degrading proteases of Prevotella intermedia and Prevotella nigrescens." Oral Microbiology and Immunology 10, no. 3 (June 1995): 138–45. http://dx.doi.org/10.1111/j.1399-302x.1995.tb00134.x.

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21

Bernal, L. A., E. Guillot, C. Paquet, and C. Mouton. "β-lactamase-producing strains in the species Prevotella intermedia and Prevotella nigrescens." Oral Microbiology and Immunology 13, no. 1 (February 1998): 36–40. http://dx.doi.org/10.1111/j.1399-302x.1998.tb00748.x.

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22

Gmür, Rudolf, and Thomas Thurnheer. "Direct quantitative differentiation between Prevotella intermedia and Prevotella nigrescens in clinical specimens." Microbiology 148, no. 5 (May 1, 2002): 1379–87. http://dx.doi.org/10.1099/00221287-148-5-1379.

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Falcón-Pasapera, Guido Sebastián, and Britto Ebert Falcón-Guerrero. "Prevotella intermedia y enfermedad periodontal en embarazadas." Revista Odontológica Basadrina 4, no. 1 (June 26, 2020): 54–58. http://dx.doi.org/10.33326/26644649.2020.4.1.916.

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La enfermedad periodontal causa la pérdida de dientes en las mujeres embarazadas. Existe mucha evidencia que relaciona al embarazo con el desarrollo de las enfermedades periodontales, debido a la relación entre las hormonas sexuales con los tejidos periodontales; sin embargo, lo que llama la atención es la afinidad que hay entre estas hormonas con la prevalencia de la bacteria Prevotella intermedia. En tal sentido, se realizó una revisión bibliográfica actualizada con el objetivo de analizar si existe relación entre la presencia de la Prevotella intermedia con el desarrollo y la magnificación de las enfermedades periodontales en las mujeres embarazadas. Tomando en cuenta la evidencia científica analizada, se llegó a la conclusión que sí hay relación entre la Prevotella intermedia y el desarrollo y magnificación de las enfermedades periodontales durante la etapa del embarazo. Por lo cual, es siempre recomendable realizar la etapa preventiva y no quirúrgica del tratamiento.
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Beem, J. E., W. E. Nesbitt, and K. P. Leung. "Identification of hemolytic activity in Prevotella intermedia." Oral Microbiology and Immunology 13, no. 2 (April 1998): 97–105. http://dx.doi.org/10.1111/j.1399-302x.1998.tb00719.x.

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Guan, Su-Min, Hideki Nagata, Satoshi Shizukuishi, and Jun-Zheng Wu. "Degradation of human hemoglobin by Prevotella intermedia." Anaerobe 12, no. 5-6 (October 2006): 279–82. http://dx.doi.org/10.1016/j.anaerobe.2006.09.001.

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Bulkacz, Jaime, and Kym F. Faull. "Multiple extracellular phospholipase activities from Prevotella intermedia." Anaerobe 15, no. 3 (June 2009): 91–94. http://dx.doi.org/10.1016/j.anaerobe.2008.12.006.

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Fukuoka, Mami, Yosuke Aoki, Shinichiro Hayashi, Kohei Nagasawa, Katsuhiro Aita, Takao Hotokebuchi, and Toshimi Satoh. "Pyogenic vertebral osteomyelitis caused by Prevotella intermedia." Journal of Infection and Chemotherapy 8, no. 2 (2002): 182–84. http://dx.doi.org/10.1007/s101560200032.

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Agatha, Velia, Calvin Kurnia, and Vinna Kurniawati Sugiaman. "Aktivitas antibakteri ekstrak kulit jeruk nipis (Citrus aurantifolia) terhadap bakteri Prevotella intermediaAntibacterial activity of lime (Citrus aurantifolia) peel extract towards Prevotella intermedia." Jurnal Kedokteran Gigi Universitas Padjadjaran 33, no. 2 (August 31, 2021): 167. http://dx.doi.org/10.24198/jkg.v33i2.33226.

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Pendahuluan: Prevotella intermedia merupakan salah satu bakteri utama pada penyakit periimplantitis. Periimplantitis merupakan inflamasi jaringan lunak dan keras disekitar implan yang dapat dicegah menggunakan ekstrak tanaman antibakteri. Salah satunya yaitu kulit jeruk nipis, yang dapat menghambat proses inflamasi karena mengandung alkaloid, steroid, saponin, flavonoid, tanin sebagai senyawa antibakteri. Penelitian ini bertujuan untuk mengetahui peranan antibakteri kulit jeruk nipis dengan mengukur Konsentrasi Hambat Minimal (KHM) dan Konsentrasi Bunuh Minimal (KBM) ekstrak kulit jeruk nipis terhadap pertumbuhan bakteri Prevotella intermedia. Metode: Eksperimental laboratorium dengan rancangan penelitian post test only control group design. Pengujian KHM dan KBM dilakukan dengan metode dilusi, kulit jeruk nipis dimaserasi menggunakan pelarut etanol 70% sehingga didapatkan ekstrak kulit jeruk nipis dengan konsentrasi 0,78, 1,56, 3,125, 6,25, 12,5, 25, 50, dan 100% dengan chlorhexidine sebagai kontrol positif dan akuades sebagai kontrol negatif. Media kultur bakteri menggunakan Tripton Soya Agar. Hasil: Berdasarkan analisis statistik menggunakan Kruskal Wallis menunjukkan perbedaan penurunan jumlah koloni bakteri yang signifikan (p=0,0001) pada KBM dan KHM dari berbagai konsentrasi ekstrak kulit jeruk nipis terhadap pertumbuhan bakteri Prevotella intermedia, uji lanjutan Mann Whitney menunjukkan perbedaan penurunan jumlah koloni bakteri yang signifikan (p=0,021) antar masing-masing konsentrasi dan kelompok kontrol. Simpulan: Konsentrasi hambat minimal ekstrak kulit jeruk nipis terhadap bakteri Prevotella intermedia 12,5%, konsentrasi bunuh minimalnya 25%. Kata kunci: agen antibakteri; ekstrak jeruk nipis; Prevotella intermediaABSTRACTIntroduction: Prevotella intermedia is one of the main bacteria in periimplantitis. Periimplantitis is inflammation of the soft and hard tissues around the implant that can be prevented using antibacterial plant extracts. One of them is lime peel, which can inhibit the inflammatory process due to its alkaloids, steroids, saponins, flavonoids, and tannins as antibacterial compounds. This study was aimed to determine the antibacterial activity of lime peel by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of lime peel extract on the growth of Prevotella intermedia. Methods: Experimental laboratory with post-test only control group design. The MIC and MBC tests were performed by the dilution method. The lime peel was macerated using 70% ethanol solvent to obtain lime peel extract with concentrations of 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, and 100% with chlorhexidine as a positive control and aquadest as a negative control. Bacterial culture media using Tripton Soy Agar. Results: Based on statistical analysis using Kruskal Wallis showed a significant difference in the decrease of the number of bacterial colonies (p=0.0001) in MBC and MIC from various concentrations of lime peel extract on the growth of Prevotella intermedia bacteria. Furthermore, the Mann Whitney follow-up test showed differences in the decrease of the number of bacterial colonies, which was significant (p=0.021) between each concentration and control group. Conclusions: The minimum inhibitory concentration of lime peel extract towards the growth of Prevotella intermedia was 12.5%, with the minimum bactericidal concentration of 25%. Keywords: antibacterial agent; citrus extracts; Prevotella intermedia
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Andrés, María T., Whasun O. Chung, Marilyn C. Roberts, and José F. Fierro. "Antimicrobial Susceptibilities ofPorphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens spp. Isolated in Spain." Antimicrobial Agents and Chemotherapy 42, no. 11 (November 1, 1998): 3022–23. http://dx.doi.org/10.1128/aac.42.11.3022.

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The susceptibilities of 143 Porphyromonas gingivalis,Prevotella intermedia, and Prevotella nigrescens isolates to 18 antimicrobial agents were tested. AllP. gingivalis isolates were susceptible. In contrast, somePrevotella spp. (17%) were resistant to β-lactams, erythromycin, clindamycin, or tetracycline and carried resistance genes, ermF or tetQ, or β-lactamases.
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Pearce, M. A., D. A. Devine, R. A. Dixon, and T. J. M. Van Steenbergen. "Genetic heterogeneity in Prevotella intermedia , Prevotella nigrescens , Prevotella corporis and related species isolated from oral and nonoral sites." Oral Microbiology and Immunology 15, no. 2 (April 2000): 89–95. http://dx.doi.org/10.1034/j.1399-302x.2000.150204.x.

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Nesbitt, W. E., H. Fukushima, K. P. Leung, and W. B. Clark. "Coaggregation of Prevotella intermedia with oral Actinomyces species." Infection and Immunity 61, no. 5 (1993): 2011–14. http://dx.doi.org/10.1128/iai.61.5.2011-2014.1993.

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Smalley, John W., Jack Silver, Andrew J. Birss, Robert Withnall, and Philip J. Titler. "The haem pigment of the oral anaerobes Prevotella nigrescens and Prevotella intermedia is composed of iron(III) protoporphyrin IX in the monomeric form." Microbiology 149, no. 7 (July 1, 2003): 1711–18. http://dx.doi.org/10.1099/mic.0.26258-0.

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The haem pigment of Porphyromonas gingivalis is composed of μ-oxo bishaem, [Fe(III)PPIX]2O, but the nature of that generated by Prevotella species has not been established. Mössbauer, Raman and UV-visible spectrophotometry were used to characterize the haem pigment of Prevotella intermedia and Prevotella nigrescens. Mössbauer and Raman spectroscopy revealed the major haem species to be monomeric iron protoporphyrin IX, Fe(III)PPIX.OH (haematin). The terminal growth pH of both species on blood agar was between 5·8 and 6·0, which favours the formation and maintenance of monomeric Fe(III)PPIX.OH. Incubation of Pr. nigrescens and Pr. intermedia with oxyhaemoglobin at pH 6·5 resulted in formation of aquomethaemoglobin which was degraded to generate Fe(III)PPIX.OH which in turn became cell-associated, whilst incubation at pH 7·5 resulted in formation of [Fe(III)PPIX]2O. It is concluded that both Prevotella species degrade oxyhaemoglobin to form [Fe(III)PPIX]2O as an intermediate, which is converted to Fe(III)PPIX.OH through a depression in pH. The low pH encourages cell-surface deposition of insoluble Fe(III)PPIX.OH which would act as a barrier against oxygen and reactive oxygen species, and also protect against H2O2 through its inherent catalase activity.
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Peck, Seung-Yup, Young Ku, In-Cheol Rhyu, Byung-Do Hahm, Soo-Boo Han, Sang-Mook Choi, and Chong-Pyoung Chung. "The Frequency of Detecting Prevotella intermedia and Prevotella nigrescens in Korean Adult Periodontitis Patients." Journal of the Korean Academy of Periodontology 30, no. 2 (2000): 419. http://dx.doi.org/10.5051/jkape.2000.30.2.419.

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Ishikawa, Hiroyuki, and Masaaki Okamoto. "Incidence and Distribution of Prevotella intermedia, Prevotella nigrescens and Porphyromonas gingivalis Isolated from Gingivitis." Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 41, no. 3 (1999): 277–86. http://dx.doi.org/10.2329/perio.41.277.

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Sakamoto, M., H. Kumada, N. Hamada, Y. Takahashi, M. Okamoto, M. A. Bakir, and Y. Benno. "Prevotella falsenii sp. nov., a Prevotella intermedia-like organism isolated from monkey dental plaque." INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 59, no. 2 (February 1, 2009): 319–22. http://dx.doi.org/10.1099/ijs.0.002626-0.

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36

Bae, K. S., J. C. Baumgartner, T. R. Shearer, and L. L. David. "RS 61 Occurrence of Prevotella intermedia and Prevotella nigrescens in infections of endodontic origin." Journal of Endodontics 21, no. 4 (April 1995): 230. http://dx.doi.org/10.1016/s0099-2399(06)80630-8.

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37

Lie, M. A., G. A. Van Der Weijden, M. F. Timmerman, B. G. Loos, T. J. M. Van Steenbergen, and U. Van Der Velden. "Occurrence of Prevotella intermedia and Prevotella nigrescens in relation to gingivitis and gingival health." Journal of Clinical Periodontology 28, no. 2 (February 2001): 189–93. http://dx.doi.org/10.1034/j.1600-051x.2001.028002189.x.

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38

Sanai, Yasaman, G. Rutger Persson, Jacqueline R. Starr, Henrique S. Luis, Mario Bernardo, Jorge Leitao, and Marilyn C. Roberts. "Presence and antibiotic resistance of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens in children." Journal of Clinical Periodontology 29, no. 10 (October 2002): 929–34. http://dx.doi.org/10.1034/j.1600-051x.2002.291008.x.

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39

Robertson, Katherine L., David B. Drucker, Anthony S. Blinkhorn, and Robin M. Davies. "A Comparison of Techniques used to Distinguish Strains of Prevotella intermedia from Prevotella nigrescens." Anaerobe 5, no. 3-4 (June 1999): 119–22. http://dx.doi.org/10.1006/anae.1999.0206.

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40

Leung, K. P., and Barbara A. Torres. "Prevotella intermedia Stimulates Expansion of Vβ-Specific CD4+ T Cells." Infection and Immunity 68, no. 9 (September 1, 2000): 5420–24. http://dx.doi.org/10.1128/iai.68.9.5420-5424.2000.

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ABSTRACT Recent evidence suggests that certain periodontal pathogens preferentially stimulate T cells expressing specific variable regions on the β chain (Vβ) of the T-cell receptor, which may indicate the presence of a superantigen. Superantigens are microbial proteins that activate large numbers of CD4+ T cells in a Vβ-specific manner. The purpose of this study was to determine whether Prevotella intermedia, a putative periodontal pathogen, activates populations of specific Vβ on CD4+ T cells. Among the bacterial strains tested,P. intermedia strain 17, a clinical isolate, induced the strongest proliferative response in peripheral blood mononuclear cells. Antibodies raised against whole cells of this organism blocked the proliferative activity. P. intermedia-induced proliferation was T-cell specific and required the presence of antigen-presenting cells. Flow cytometric analysis showed that CD4+ T-cell subsets expressing Vβ8, Vβ12, and Vβ17 expanded in response to P. intermedia strain 17. The ability of P. intermedia to stimulate CD4+-T-cell proliferation was further supported by the production profiles of key T-cell cytokines, gamma interferon and interleukin-2. The data collectively suggest that certain strains ofP. intermedia can activate Vβ-specific T cells in a manner similar to that of other known microbial superantigens.
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Moon, Ji-Hoi, Cheul Kim, Hee-Su Lee, Sung-Woon Kim, and Jin-Yong Lee. "Antibacterial and antibiofilm effects of iron chelators against Prevotella intermedia." Journal of Medical Microbiology 62, no. 9 (September 1, 2013): 1307–16. http://dx.doi.org/10.1099/jmm.0.053553-0.

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Prevotella intermedia, a major periodontopathogen, has been shown to be resistant to many antibiotics. In the present study, we examined the effect of the FDA-approved iron chelators deferoxamine (DFO) and deferasirox (DFRA) against planktonic and biofilm cells of P. intermedia in order to evaluate the possibility of using these iron chelators as alternative control agents against P. intermedia. DFRA showed strong antimicrobial activity (MIC and MBC values of 0.16 mg ml−1) against planktonic P. intermedia. At subMICs, DFRA partially inhibited the bacterial growth and considerably prolonged the bacterial doubling time. DFO was unable to completely inhibit the bacterial growth in the concentration range tested and was not bactericidal. Crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that DFRA significantly decreased the biofilm-forming activity as well as the biofilm formation, while DFO was less effective. DFRA was chosen for further study. In the ATP-bioluminescent assay, which reflects viable cell counts, subMICs of DFRA significantly decreased the bioactivity of biofilms in a concentration-dependent manner. Under the scanning electron microscope, P. intermedia cells in DFRA-treated biofilm were significantly elongated compared to those in untreated biofilm. Further experiments are necessary to show that iron chelators may be used as a therapeutic agent for periodontal disease.
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Borsanelli, Ana Carolina, Elerson Gaetti-Jardim Júnior, Christiane Marie Schweitzer, Jürgen Döbereiner, and Iveraldo S. Dutra. "Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis." Pesquisa Veterinária Brasileira 35, no. 10 (October 2015): 829–34. http://dx.doi.org/10.1590/s0100-736x2015001000002.

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Abstratc: Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent-culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non-injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis.
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Tomazinho, Luiz Fernando, and Mario J. Avila-Campos. "Detection of Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Prevotella nigrescens in chronic endodontic infection." Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 103, no. 2 (February 2007): 285–88. http://dx.doi.org/10.1016/j.tripleo.2006.05.010.

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44

Takahashi, N., and T. Sato. "Dipeptide utilization by the periodontal pathogens Porphyromonas gingivalis , Prevotella intermedia , Prevotella nigrescens and Fusobacterium nucleatum." Oral Microbiology and Immunology 17, no. 1 (February 2002): 50–54. http://dx.doi.org/10.1046/j.0902-0055.2001.00089.x.

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45

Mättö, J., S. Asikainen, M. ‐L Väisänen, M. Rautio, M. Saarela, P. Summanen, S. Finegold, and H. Jousimies‐Somer. "Role of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens in Extraoral and Some Odontogenic Infections." Clinical Infectious Diseases 25, s2 (September 1997): S194—S198. http://dx.doi.org/10.1086/516205.

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46

Hafström, C., and G. Dahlén. "Pathogenicity of Prevotella intermedia and Prevotella nigrescens isolates in a wound chamber model in rabbits." Oral Microbiology and Immunology 12, no. 3 (June 1997): 148–54. http://dx.doi.org/10.1111/j.1399-302x.1997.tb00371.x.

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47

Tamura, M., Y. Hirano, K. Koruda, F. Kuwata, and K. Hayashi. "Effects of zinc and copper on adhesion and hemagglutination of Prevotella intermedia and Prevotella nigrescens." Oral Microbiology and Immunology 20, no. 6 (December 2005): 339–43. http://dx.doi.org/10.1111/j.1399-302x.2005.00234.x.

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48

Masakiyo, Yoshiaki, Akihiro Yoshida, Yasuyuki Shintani, Yusuke Takahashi, Toshihiro Ansai, and Tadamichi Takehara. "The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization." Anaerobe 16, no. 3 (June 2010): 265–69. http://dx.doi.org/10.1016/j.anaerobe.2009.11.003.

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49

Liu, Juan, Li Cui, Xinmin Yan, Xinyuan Zhao, Jinluo Cheng, Lei Zhou, Jianbo Gao, Zhong Cao, Xinhua Ye, and Shen Hu. "Analysis of Oral Microbiota Revealed High Abundance of Prevotella Intermedia in Gout Patients." Cellular Physiology and Biochemistry 49, no. 5 (2018): 1804–12. http://dx.doi.org/10.1159/000493626.

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Background/Aims: Microbes reside in a number of body sites, including the oral cavity, and are associated with the progression of many systemic diseases. In this study, we aimed to investigate the effects of gout and hyperuricemia (HUA) on the composition of oral microbiomes. Methods: Analysis of the oral microbiota from 12 gout patients, 11 HUA patients, and 19 healthy control subjects was performed using a deep sequencing approach, and validation of significant changes in Prevotella intermedia and Serratia marcescens in new patient cohorts was performed using quantitative PCR (qPCR). Results: Our analysis indicated that both gout and HUA significantly altered the composition of the oral microbiome in patients. Patients with gout or HUA had significantly greater levels of salivary Prevotella intermedia but significantly lower levels of Serratia marcescens than healthy control subjects. Conclusion: We demonstrated the association between the oral microbiome and gout and HUA for the first time. In particular, 16S sequencing and qPCR analysis revealed significantly higher levels of oral Prevotella intermedia in gout/HUA patients, which suggests that these patients might be at risk for the development of periodontitis.
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Iida, Toshiya. "Purification and Characterization of Acid Phosphatase from Prevotella intermedia ATCC 25611." Journal of the Kyushu Dental Society 52, no. 1 (1998): 125–34. http://dx.doi.org/10.2504/kds.52.125.

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