Academic literature on the topic 'Prevotella intermedia'
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Journal articles on the topic "Prevotella intermedia"
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Takahashi, N., and T. Yamada. "Glucose metabolism by Prevotella intermedia and Prevotella nigrescens." Oral Microbiology and Immunology 15, no. 3 (June 2000): 188–95. http://dx.doi.org/10.1034/j.1399-302x.2000.150307.x.
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FRANDSEN, E. V. G., K. POULSEN, and M. KILIAN. "Confirmation of the Species Prevotella intermedia and Prevotella nigrescens." International Journal of Systematic Bacteriology 45, no. 3 (July 1, 1995): 429–35. http://dx.doi.org/10.1099/00207713-45-3-429.
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Kalfas, S., Z. Tigyi, M. Wikström, and A. S. Naidu. "Laminin binding to Prevotella intermedia." Oral Microbiology and Immunology 7, no. 4 (August 1992): 235–39. http://dx.doi.org/10.1111/j.1399-302x.1992.tb00031.x.
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Leung, K. P., H. Fukushima, W. E. Nesbitt, and W. B. Clark. "Prevotella intermedia fimbriae mediate hemagglutination." Oral Microbiology and Immunology 11, no. 1 (February 1996): 42–50. http://dx.doi.org/10.1111/j.1399-302x.1996.tb00335.x.
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Anumala, Deepa, Mohan Kumar Pasupuleti, and Ravindra Reddy Nagireddy. "Detection and Characterization of Prevotella Intermedia and Its In Vitro Susceptibility to Selected Antimicrobial Agents in Chronic Periodontitis and Acute Myocardial Infarction." December 2019 3, no. 1 (February 22, 2019): 1–6. http://dx.doi.org/10.26810/perioj.2019.a1.
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Background: Periodontal disease has been reported to play a causative role in acute myocardial infarction (AMI), which may add to the various risk factors associated with coronary heart disease. The objective of the present study was to investigate the presence of Prevotella intermedia – an established periodontal pathogen – in subgingival plaque samples of chronic periodontitis and AMI patients in order to identify a possible association, and to evaluate the susceptibility of Prevotella intermedia to nine antimicrobial agents. Methods: After undergoing screening for eligibility, a total of 50 subjects were included in the present study. Twenty patients were diagnosed with AMI and generalized chronic periodontitis (Group I), 20 patients were diagnosed with only AMI (Group II), and 10 subjects were healthy controls (Group III). The isolated Prevotella intermedia strains were tested for susceptibility to bacitracin, chloramphenicol, penicillin G, polymyxin, gentamycin, neomycin, tetracycline, cefotaxime, and cefoxitin using an antibiotic zonescale to determine minimum inhibitory concentrations (MICs). Results: Periodontal pathogens were identified by phenotypic and enzymatic methods. The mean bacterial load of Prevotella intermedia species was higher in Group I compared to Group II and Group III. It was also found that pencillin G, gentamycin, neomycin, tetracycline, cefotaxime, and cefoxitin inhibited 90% of Prevotella intermedia, whereas bacitracin, chloramphenicol, and polymyxin inhibited 80% of Prevotella intermedia. Thus, only 10% of Prevotella intermedia were resistant to these antibiotics. Conclusion: The present study confirms that Prevotella intermedia is associated with chronic periodontitis and AMI.
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Mättö, Jaana, Sirkka Asikainen, Marja-Liisa Väisänen, Birgitta Von Troil-Lindén, Eija Könönen, Maria Saarela, Kari Salminen, Sydney M. Finegold, and Hannele Jousimies-Somer. "β-Lactamase Production in Prevotella intermedia,Prevotella nigrescens, and Prevotella pallensGenotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents." Antimicrobial Agents and Chemotherapy 43, no. 10 (October 1, 1999): 2383–88. http://dx.doi.org/10.1128/aac.43.10.2383.
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ABSTRACT The present study investigated the β-lactamase production of 73Prevotella intermedia, 84 Prevotella nigrescens, and 14 Prevotella pallens isolates and their in vitro susceptibilities to six antimicrobial agents. TheP. intermedia and P. nigrescens isolates were recovered from oral and extraoral samples obtained from subjects in two geographic locations from 1985 to 1995. The clonality of the β-lactamase-positive and β-lactamase-negative isolates and the clustering of the genotypes were studied by arbitrarily primed-PCR fingerprinting. β-Lactamase production was detected in 29% ofP. intermedia isolates, 29% of P. nigrescensisolates, and 57% of P. pallens isolates. No difference in the frequencies of β-lactamase production by P. intermedia and P. nigrescens between isolates from oral and extraoral sites, between isolates obtained at different time periods, or between P. intermedia isolates from different geographic locations was observed. However, the P. nigrescens isolates from the United States were significantly more frequently (P = 0.015) β-lactamase positive than those from Finland. No association between the genotypes and β-lactamase production or between the genotypes and the sources of the isolates was found. The penicillin G MICs at which 90% of the isolates were inhibited were 8 μg/ml for P. intermedia, 8 μg/ml for P. nigrescens, and 16 μg/ml for P. pallens. For the β-lactamase-negative isolates, the corresponding values were 0.031, 0.031, and 0.125 μg/ml, and for the β-lactamase-positive isolates, the corresponding values were 16, 8, and 32 μg/ml. All isolates were susceptible to amoxicillin-clavulanate, cefoxitin, metronidazole, azithromycin, and trovafloxacin. The MICs of amoxicillin-clavulanate and cefoxitin were relatively higher for the β-lactamase-positive population than for the β-lactamase-negative population.
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Dahlén, G. "Prevotella intermedia and Prevotella nigrescens serotypes, ribotypes and binding characteristics." FEMS Microbiology Letters 138, no. 1 (April 15, 1996): 89–95. http://dx.doi.org/10.1016/0378-1097(96)00095-x.
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Luong, N., J. Tsai, and C. Chen. "Susceptibilities of Eikenella corrodens,Prevotella intermedia, and Prevotella nigrescensClinical Isolates to Amoxicillin and Tetracycline." Antimicrobial Agents and Chemotherapy 45, no. 11 (November 1, 2001): 3253–55. http://dx.doi.org/10.1128/aac.45.11.3253-3255.2001.
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ABSTRACT The AB Biodisk Etest showed that 106 (100%) and 98 (92%) isolates of Eikenella corrodens were susceptible to amoxicillin and tetracycline, respectively. Twenty-three (68%) Prevotella intermedia isolates and 14 (67%) Prevotella nigrescens isolates were susceptible to amoxicillin. Seventy-nine percent of the P. intermedia isolates and 67% of theP. nigrescens isolates were susceptible to tetracycline. A higher percentage of β-lactamase-producing isolates of P. intermedia and P. nigrescens were identified with selective agar containing amoxicillin than with nonselective agar.
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Tanaka, Shoji, Mikako Yoshida, Yukio Murakami, Takako Ogiwara, Masao Shoji, Satoko Kobayashi, Sigeru Watanabe, Mamoru Machino, and Seiichiro Fujisawa. "The Relationship of Prevotella intermedia, Prevotella nigrescensand Prevotella melaninogenica in the Supragingival Plaque of Children, Caries and Oral Malodor." Journal of Clinical Pediatric Dentistry 32, no. 3 (April 1, 2008): 195–200. http://dx.doi.org/10.17796/jcpd.32.3.vp657177815618l1.
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Purpose: A relationship between the distribution of periodontal bacteria species and malodor in children has not been sufficiently investigated. The present study was undertaken to determine the presence of 3 periodontopathic bacteria (Prevotella spp. P. intermedia, P. nigrescens, P. melaninogenica) in the supragingival plaques of 3 to 16-year-old children with different oral health conditions and oral malodor. Methods: The number of decayed and filled primary teeth (df) and Decayed, Missing and Filled permanent teeth (DMF),Papillary Marginal and Attached gingivitis (PMA) index, Oral Hygiene Index (OHI), and oral malodor of each subject were determined prior to the collection of supragingival plaques. Three periodontopathic bacteria(P. intermedia, P. nigrescens, P. melaninogenica ) in supragingival plaques were detected by using an immunoslot blot assay with monoclonal antibodies specific for each microorganism. Findings: The frequencies of periodontopathic bacteria in children with and without caries were not significantly different from each other. Positivity for P. intermedia, but not for P. nigrescens or P. melaninogenica was correlated with oral malodor. Oral malodor was also correlated with the debris index, a component of OHI. The group with the higher OHI showed a higher prevalence of periodontopathic bacteria. For the 3 periodontopathic bacteria in the subjects tested, plaques positive for any of them were not age related. However,the frequencies of all 3 periodontopathic bacteria were the highest in the 3-6-year olds. Conclusion: The supragingival plaques in children can harbor 3 species of periodontopathic bacteria, P. intermedia,P. nigrescens, and P. melaninogenica.
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Stubbs, S., MAO Lewis, RJ Waddington, and G. Embery. "Hydrolytic and depolymerising enzyme activity of Prevotella intermedia and Prevotella nigrescens." Oral Diseases 2, no. 4 (June 28, 2008): 272–78. http://dx.doi.org/10.1111/j.1601-0825.1996.tb00237.x.
Full textDissertations / Theses on the topic "Prevotella intermedia"
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Mättö, Jaana. "Prevotella intermedia, Prevotella nigrescens and closely related anaerobes in oral and extraoral infections." Helsinki : Institute of Dentistry, Research Laboratory, University of Helsinki, 1999. http://catalog.hathitrust.org/api/volumes/oclc/45193770.html.
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Barbosa, Graziela Murta. "Interações de isolados clínicos de Prevotella intermedia e Prevotella nigrescens com Porphyromonas gingivalis na formação de biofilmes." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-26062014-153511/.
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Prevotella intermedia e Prevotella nigrescens são espécies comumente associadas Porphyromonas gingivalis. os objetivos foram verificar a co-agregação entre cepas de P. intermedia, P. nigrescens e P. gingivalis; quantificar a biomassa, avaliar a proporção dos microrganismos nos biofilmes mistos; verificar a interferência do co-cultivo pela técnica de dois compartimentos, avaliar os biofilmes em ensaios de Hibridização In Situ (FISH) e verificar o papel dos genes PINA0102 e PIN0398 de P. intermedia no biofilme. Assim, 9 isolados clínicos de P. intermedia e 5 de P. nigrescens, as cepas padrão P. intermedia 17, P. intermedia 25611, P. nigrescens 33563, P. gingivalis W83, P. gingivalis 33277, os mutantes Pi17D0398 e Pi17D0102 foram avaliados em variados consórcios. Os resultados mostraram que as interações investigadas são cepa específicas.Prevotella intermedia and Prevotella nigrescens are commonly associated with periodontal diseases. The goals of this study were to determine the co-aggregation of strains of P. intermedia, P. nigrescens and P. gingivalis; to quantify the biomass of these associations, to evaluate the ratio of these microorganisms in heterotypic biofilms, to verify the modulation of biofilms in co-culture using a trans-well system; to evaluate the structure of the biofilms by Fluorescent Hybridization In Situ (FISH) and to determine the role of genes PINA0102 and PIN0398 of P. intermedia in the modulation of its biofilm. Therefore, 9 clinical isolates of P. intermedia, 5 of P. nigrescens, type strains P. intermedia 17, P. intermedia 25611, P. nigrescens 33563, P. gingivalis W83, P. gingivalis 33277 and mutant strains Pi17D0398 and Pi17D0102 were grown in consortia of two strains. Our data demonstrate that the associations of P. intermedia, P. nigrescens and P. gingivalis are strain-specific.
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Hudji, Baraa. "Susceptibility of the Anaerobic Bacterial species Parvimonas micra, Prevotella intermedia and Prevotella nigrescens to selected Antibiotics – Then and Now." Thesis, Umeå universitet, Institutionen för odontologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-129981.
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The present study evaluates the susceptibility to antibiotics of three anaerobic bacteria; Prevotella intermedia, Prevotella nigrescens and Parvimonas micra, bacterial species commonly found in root canal infections. Strains from two different time periods were tested against benzylpenicillin, amoxicillin and metronidazole. There are two collections of each bacterial species in this study. One of the collections is from 1976. Other collection includes the same bacterial species collected in recent years, (2009 to 2014). To identify all three bacteria species, the PCR (Polymerase Chain Reaction) method with species specific primers was used. To identify the minimal inhibitory concentrations (MIC) of selected antibiotics Etest strips were used according to Eucasts recommendations. The MIC values differ for P. micra, P. intermedia and P. nigrescens when exposed to the selected antibiotics. The new collection of P. micra showed higher MIC-values for amoxicillin and metronidazole than the old collection. The recent collection of P. intermedia showed higher MIC values for metronidazole compared to the old collection. On the other hand P. nigrescens showed no difference in MIC values between the two collections, although one recently isolated strain of P. nigrescens showed noticeable difference in MIC-values for benzylpenicillin and amoxicillin antibiotics compared to the old collection. The results indicate a susceptibility change for the tested antibiotics. Antibiotics susceptibility has changed during a 40 -year period for the selected bacterial species and it also shows that different strains of bacteria, although belonging to the same species, can have different susceptibility to antibiotics.
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Alves, Ana Claudia Braga Amoras. "Analise da diversidade genetica de Prevotella intermedia em individuos com doença periodontal." [s.n.], 2003. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288637.
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Orientador: Reginaldo Bruno GonçalvesTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: O objetivo deste trabalho foi avaliar a diversidade genética da espécie Prevotella intermedia em indivíduos com doença periodontal, por meio de duas técnicas, a reação em cadeia da polimerase com primers arbitrários (AP-PCR) e análise heteroduplex de produtos amplificados pela reação em cadeia da polimerase (PCR). Amostras subgengivais foram obtidas de sítios com e sem destruição periodontal de 36 voluntários. Após o cultivo e identificação bacteriana, os isolados de P. intermedia foram submetidos à técnica de genotipagem utilizando AP-PCR. Adicionalmente, as amostras clínicas subgengivais, com resultado positivo para o cultivo de P. intermedia, foram submetidas à reação em cadeia da polimerase (PCR) com primer espécie específico e em seguida, os produtos amplificados foram submetidos à análise heteroduplex. Os resultados revelaram que os anaeróbios pigmentados negros foram mais freqüentes em sítios doentes, sendo a P. intermedia a mais prevalente dentre as espécies isoladas. A maioria dos indivíduos foi colonizada por um a dois tipos de genótipos, com relação positiva entre o número de clones e a profundidade de sondagem. Não houve detecção de genótipos idênticos entre os sítios de isolamento, em um mesmo ambiente bucal. Na análise interindivíduos, nenhum voluntário compartilhou o mesmo perfil eletroforético, indicando ampla heterogeneidade genética da espécie. Coincidindo com a técnica de AP -PCR, a análise heteroduplex detectou a presença de uma a dois clones distintos de P. intermedia nas amostras clínicas. Em conclusão, os indivíduos com doença periodontal abrigaram poucos tipos clonais e a coincidência de perfil genético entre sítios de um mesmo indivíduo e entre indivíduos não foi observada
Abstract: The aim of this study was to assess the genotypic diversity of P. intermedia on subjects with periodontal disease using two techniques, the arbitrarily primed polymerase chain reaction (AP-PCR) method and heteroduplex analysis from products amplified by polymerase chain reaction (PCR). Subgingival plaque samples were taken from the sites with and without periodontal destruction in 36 volunteers. After the culturing and identification procedures, the AP-PCR method was used for the genotypic characterization of P. intermedia strains. In addition, the clinical samples with a positive result for culture of the P. intermedia were amplified by polymerase chain reaction (PCR) method with a specie-specific primer and, after that, the PCR products were compared by the heteroduplex analysis. The results indicated that black-pigmented anaerobes were frequently cultured from periodontal diseased sites and P. intermedia were the most prevalent among the species recovered. Most of the subjects harbored between one to two distinct genotypes of P. intermedia, with a positive relation between numbers of genotypes and pocket probing depth. No matching of P. intermedia genotypes was observed between periodontal sites of the same individual. Interindividual genetic diversity has demonstrated absence of identical clones, indicating a high level of genetic diversity. The heteroduplex analyses of PCR products revealed that the majority of clinical samples harbored one to two clonal types, just like the AP-PCR technique. In conclusion, the subjects with periodontal disease harbored few clonal types and no identical genotypes between sites in the same individual and between individuals were found
Doutorado
Microbiologia e Imunologia
Doutor em Biologia Buco-Dental
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Beer, Isabelle Marion [Verfasser], and Gerd [Akademischer Betreuer] Döring. "Nachweis von Prevotella intermedia und Antikörperbildung gegen P. intermedia bei Patienten mit Cystischer Fibrose / Isabelle Marion Beer ; Betreuer: Gerd Döring." Tübingen : Universitätsbibliothek Tübingen, 2011. http://d-nb.info/1161734732/34.
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Higashi, Daniela. "Modulação do biofilme de Porphyromonas gingivalis pela associação com Streptococcus gordonii e com Prevotella intermedia." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-10042015-123236/.
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P. gingivalis é um dos principais patógenos da doença periodontal, é encontrado em biofilmes orais com S. gordonii e P. intermedia e em células endoteliais da artéria coronária in vivo. P. gingivalis necessita de ferro em seu metabolismo e pode usar certas proteínas do hospedeiro como fontes deste íon em ambientes limitantes. Assim, este estudo investigou o papel dos genes PGN0741/PG0637 (receptor dependente de TonB) e PGN0531/PG1380 (fvW) de P. gingivalis na formação de biofilme em diferentes concentrações de ferro, em biofilmes mistos com S. gordonii e P. intermedia, e na adesão e invasão de células endoteliais da artéria coronária. Os resultados mostraram divergências no papel dos genes TonB e fvW na formação dos monobiofilmes e mistos e em diferentes concentrações de ferro, demonstrando uma relação cepa-dependente. Na adesão, fvW se mostrou importante para ambas cepas, mas na persistência apenas para P. gingivalis W83. Este trabalho enfatiza, assim, a necessidade do uso de mais de uma cepa de P. gingivalis no estudo do papel de genes em ensaios experimentais.P. gingivalis is one of the major pathogens of periodontal diseases. It is found in oral biofilms associated with S. gordonii and P. intermedia, and inside of coronary artery endothelial cells in vivo. P. gingivalis requires iron for growth and can exploit iron-carrying proteins of the host as sources in limiting environments. Thus, this work aimed to study the role of genes PGN0741/PG0637 (TonB-dependent receptor) and PGN0531/PG1380 (fvW) of P. gingivalis in biofilm formation under different iron concentrations, in mixed biofilms with S. gordonii and P. intermedia, and in the adhesion and invasion of coronary artery endothelial cells. Our data showed discordance for the role of TonB and fvW in homo- and heterotypic biofilm formation and in different iron concentrations. The relevance of both genes was strain-dependent. Gene fvW was relevant for adhesion to endothelial cells, but only for strain W83 during persistence. Therefore, our study emphasizes the importance of using different strains for a better understanding of the role of genes in experimental assays.
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Byrne, Dominic Patrick. "Mechanisms of haem acquisition by the black-pigmenting anaerobes, Prevotella intermedia and Porphyromonas gingivalis." Thesis, University of Liverpool, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569892.
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Periodontitis describes a set of related inflammatory diseases that damage the periodontal tissues that support the teeth. It is caused by bacterial infections that, if left untreated, can lead to progressive loss of alveolar bone and subsequent loosening and loss of teeth. Periodontitis is the primary cause of tooth loss in adults and treatment of more severe forms of the disease can be both painful and costly. Black-pigmenting bacterial species, such as Porphyromonas gingivalis and Prevotella intermedia, are among the major periodontal pathogens associated with severe periodontitis. These bacteria form black pigments consisting of iron protoporphyrin IX (haem; Fe(III)PPIX) when grown on blood-containing media. Development of the haem- containing black pigments by these organisms serves an important defensive role as the intrinsic catalase activity of ferrihaem destroys H202 (Smalley et al., 2000). Furthermore, formation of the pigment by strictly anaerobic P. gingivalis is a process which consumes oxygen and promotes anaerobiosis (Smalley et al., 1998; 2002). This study sought to further characterise some of the facets of the mechanisms of haem acquisition by these two bacteria, with particular emphasis on the role of specific virulence factors (InpA of P. intermedia, HmuY and the gingipains of P. gingivalis, and LPS from both species). This resulted in identification of Interpain A (InpA), a 90 kDa cysteine protease, and homologue of Streptococcus pyogenes streptopain (SpeB), which is expressed by P. intermedia as a potential major virulence factor in haem acquisition through its ability to degrade haemoglobin, haemalbumin and haern-haemopexin. InpA-mediated proteolysis of these haemoproteins was highly dependent on experimental factors such as pH and redox potential, which profoundly affected the protease sensitivity of the haem -containing protein substrates. This suggested that InpA activity would be hugely influenced by the changing conditions of the periodontal pocket during progressive periodontitis. A unique syntrophic relationship of haem acquisition from haemoglobin was also identified between the HmuY haemophore and the K- and R- gingipains of P. gingivalis. Furthermore, InpA facilitated greater haem extraction by HmuY by promoting haemoglobin oxidation to the methaemoglobin form. This suggested a degree of mutualism and possible cross-reactivity between the haem acqui sition systems of these two bacteria which has possibly arisen on account of their close proximity within the periodontal pocket biofilm. This study also identified a possible central role for LPS in binding and storage of haem at the cell surface of these black-pigmenting bacteria and presents evidence suggesting that the lipophilic nature of this molecule, in addition to the respective pericellular pHs of P intermedia and P gingivalis cells, may affect the species of haem present in the pigments.
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Rodrigues, Italo Sarto Carvalho 1983. "Efeito de diferentes concentrações de clorexidina na periodontite induzida em ratos e a influência do cálcio na formação de biofilmes por Prevotella intermedia = Effect of chlorhexidine at multiple-doses and concentrations on experimental periodontitis in rats and impact of calcium on Prevotella intermedia surface attachment and biofilm formation." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288568.
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Orientador: Rafael Nobrega StippTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: O biofilme é uma população biológica com um elevado grau de organização, onde os microrganismos presentes formam uma comunidade estruturada, coordenada e funcional. O estudo do comportamento dos biofilmes é fundamental para melhorar as formas de controle, especialmente durante infecções, tais como as doenças periodontais. No primeiro capítulo, foram avaliados os efeitos da aplicação tópica e frequente do digluconato de clorexidina (CLX) em diferentes concentrações na periodontite induzida por ligadura nos primeiros molares de ratos. As ligaduras receberam 10 µl de soluções de CLX à 0,2%, 2%, 10%, 20% ou diluente, de quatro em quatro dias, em um total de quatro aplicações. Após eutanásia, a quantidade de células bacterianas no biofilme formado sobre a ligadura foi estimada por cultura e por PCR quantitativo. A reabsorção óssea foi mensurada em altura e área por fotografia digital e em volume por microtomógrafo. Depois de quatro dias a partir da última aplicação da CLX, as reduções bacterianas mantidas pelos tratamentos com CLX atingiram até 10-6. O grupo que recebeu CLX a 20% teve, em média, logs bacterianos 3,3× menor (p<0.01, Kruskal-Wallis). Não houve diferença estatística entre os grupos em relação à reabsorção óssea por ambos os métodos testados (p>0.05, Kruskal-Wallis), embora 55% dos sítios apresentaram menor reabsorção óssea. No segundo capítulo, foi avaliada a influência de diversas substâncias na formação de biofilme por Prevotella intermedia. Os biofilmes foram formados em placas de 48 poços contendo tratamento de superfície prévio com DNA purificado, hemina, CaCO3, Ca(OH)2, CaCl2, soro, albumina, dextrana, metionina, glicose, glutamina, KCl, complexo vitamínico, cistina ou mucina. O biofilme formado foi corado e quantificado por espectrofotometria. A arquitetura do biofilme foi visualizada por microscopia confocal de fluorescência por varredura laser. O tratamento da superfície com CaCl2 a 1 mg/cm2 permitiu a formação do biofilme em quantidade de 0,3 OD490nm (p<0.01, ANOVA Dunnet), sendo esse valor 10× superior quando a superfície foi tratada com 2,5 mg/cm2 (p<0.01, ANOVA Dunnet). As demais substâncias tiveram pouco ou nenhum impacto sobre a formação do biofilme. A visualização por microscopia confocal revelou uma comunidade estruturada e com vitalidade em toda sua espessura. Conclusões: os dados indicam que a CLX concentrada diminui a carga bacteriana na região da periodontite induzida, que reflete em menor reabsorção óssea apenas em parte das amostras. O pré-revestimento da superfície de crescimento com cálcio promove a formação de biofilme por P. intermedia
Abstract: Biofilms are biological communities with a high degree of organization, in which micro-organisms form structured, coordinated and functional communities. These biological communities are embedded in a self-created extracellular matrix. Biofilm is also associated with a high level of antimicrobial tolerance of the associated organisms. Understanding biofilm behavior is crucial to develop ways for its control during infections, such as periodontal disease. In the first chapter, topical and frequent application of various concentrations of chlorhexidine digluconate (CLX) where evaluated. Periodontitis were induced by ligature on first molars. Then, ligatures were treated with 10 µl of chlorhexidine solutions at 0.2%, 2%, 10%, 20% or diluent, every four days in a total of four applications periods. After euthanasia, bacterial loads on ligatures were estimated by both culture and qPCR. Bone resorption height and area were measured by digital photography and its volume by microtomography. Treated sites had bacterial reductions up to 10-6 cells. Treatment with 20% CLX showed mean of 3.3× lower bacterial levels (p<0.01, Kruskal-Wallis). There was no statistical difference between groups regarding bone resorption (p>0.05, Kruskal-Wallis), although 55% of the treated sites had some lower bone resorption. In the second chapter, substances that may enhance biofilm formation by Prevotella intermedia were investigated. Wells of 48-well plates were coated with DNA, hemin, CaCO3, Ca(OH)2, CaCl2, serum, albumin, dextran, methionine, glucose, glutamine, KCl, vitamin complex, cystine or mucin. Biofilms were grown for 24 h, washed, stained and quantified by spectrophotometry. Biofilm architecture and its viability were visualized by Confocal Laser Scanning Microscopy. Surfaces treated with 1 mg/cm2 of CaCl2 enhanced biofilm amount by 0.3 OD490nm (p<0.01, ANOVA Dunnet), while 2.5 mg/cm2 yielded 10-fold more biofilm mass (p<0.01, ANOVA Dunnet). Other substances had modest or no impact in biofilm mass. Confocal microscopy images showed structured and alive biofilms with no dead areas. Conclusions: concentrated CLX reduces bacterial load, which reflects in lower bone resorption in few sites. Surfaces pre-coated with calcium chloride enhance P. intermedia biofilm formation
Doutorado
Microbiologia e Imunologia
Doutor em Biologia Buco-Dental
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Hermann, Stefan [Verfasser], Wolfgang [Akademischer Betreuer] Pfister, Arndt [Akademischer Betreuer] Güntsch, and Sigrun [Akademischer Betreuer] Eick. "Nachweis der Proteasegene von Prevotella intermedia in subgingivaler Plaque / Stefan Hermann. Gutachter: Wolfgang Pfister ; Arndt Güntsch ; Sigrun Eick." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2012. http://d-nb.info/1019124601/34.
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Mackie, Tasha A., and n/a. "Influence of haem availability on the viability of Porphyromonas gingivalis and Prevotella intermedia, following exposure to reactive oxygen species." University of Otago. School of Dentistry, 2007. http://adt.otago.ac.nz./public/adt-NZDU20071211.104926.
Full textAbstract:
Objectives: This investigation adapted the LIVE/DEAD� Baclight[TM] bacterial viability stain for the quantitative determination of bacterial cell viability of the aerotolerant anaerobes Porphyromonas gingivalis ATCC 33277 and Prevotella intermedia ATCC 25611. The Live/Dead stain was used to determine the influence of haem availability on the resistance of P. gingivalis and P. intermedia to the reactive oxygen species (ROS) superoxide anion and hydrogen peroxide and compare the sensitivities between the haem-requiring periodontal bacteria to ROS. Neutrophils use oxidative and non-oxidative killing mechanisms. During phagocytosis, neutrophils kill bacteria via a respiratory burst, producing ROS. P. gingivalis and P. intermedia are oxygen-tolerant gram-negative bacteria found in the gingival crevice. These bacteria express superoxide dismutase (SOD) activity, which extends some protection against superoxide radicals.
Methods: Initially, experiments were performed to validate the reliability and accuracy of the fluorogenic Live/Dead stain using Escherichia coli ATCC 10798 (K-12), followed by experiments using P. gingivalis. The Live/Dead stain distinguishes viable:non-viable proportions of bacteria using mixtures of green (SYTO 9) and red (propidium iodide) fluorescent nucleic acid stains respectively. Bacterial cell viability was assessed with fluorescence microscopy and subsequently quantitative measurement using a fluorescence microplate reader (BMG Fluorostar plus Optima). P. gingivalis and P. intermedia colonies were subcultured from frozen cultures, in Tryptic soy broth (TSB) (Difco) and incubated anaerobically for approximately five days. They were further subcultured in pre-reduced TSB, supplemented with menadione 0.5[mu]g/ml (TSB-M) and either 5 [mu]g/ml haemin (Haem 5), 50 [mu]g/ml haemin (Haem 50) or without supplemental haemin (Haem 0). Cultures were grown anaerobically at 37�C to early stationary phase (approximately 48 hours). For experimental purposes, bacteria were harvested, washed and resuspended in 10 mM Tris-buffered saline (pH 7.5) containing peptone (TBS-P) (0.1 mg/ml), with a final adjustment to OD₅₄₀ [approximately equals] 2.0 (which corresponds to 1 x 10⁹ bacteria/ml). Bacterial suspensions were diluted ([approximately equals] 10⁸/ml) into TBS-P containing the fluorogenic viability stain (BacLight, Molecular Probes). Either pyrogallol (0.02 - 2 mM) or hydrogen peroxide (0.01 - 100 mM) was added (except to control tubes); tubes were vortexed for ten seconds and incubated at 37�C. Viability was monitored fluorimetrically for three hours.
Results: For both P. gingivalis and P. intermedia, a pyrogallol concentration of 0.2 mM resulted in 80 to 90% cell death; and a hydrogen peroxide concentration of 10 mM killed approximately 80 to 90% of cells. Irrespective of the haem status, no significant difference was determined between the overall maximum rate of killing of P. gingivalis and P. intermedia, in their response to either superoxide or hydrogen peroxide; with the exception that the P. intermedia Haem 0 group was significantly less susceptible to hydrogen peroxide than the P. gingivalis Haem 0 group. For the majority of the experiments, there was no significant difference between final bacterial cell viability in the Haem 0 and Haem 5 cells for both species, after 3 hours exposure to various concentrations of ROS. However, the Haem 50 cells showed a significant increased susceptibility (albeit, a small difference) to both hydrogen peroxide and superoxide.
Conclusions: The Live/Dead bacterial viability stain provided a valuable method to monitor "real-time" killing, avoiding the difficulties associated with culture-based methods for assessing viability. Haem availability had no clear influence on the resistance to ROS of either P. gingivalis or P. intermedia Haem 0 and Haem 5 cells. The Haem 50 cells showed a very slight increase in susceptibility to hydrogen peroxide and superoxide. Although P. intermedia may be isolated in significant numbers from healthy gingivae, as well as from periodontally diseased sites, it was no more resistant to ROS than was P. gingivalis, which is associated with periodontal lesions and difficult to cultivate from relatively healthy (more oxygenated) sites. This suggests that resistance to ROS does not contribute to the ecological distinction between these two species. The finding that haem availability did not influence sensitivity implies that these bacteria do not accumulate haem for the purpose of protection from ROS.
Books on the topic "Prevotella intermedia"
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Cookson, Adrian Lawrence. The adherence and virulence factors of Prevotella intermedia and Prevotella nigrescens. Manchester: University of Manchester, 1996.
Find full textConference papers on the topic "Prevotella intermedia"
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Mathivanan, Nithya, G. Kiruthika, and Rahavi Subashree. "Insilico screening of Prevotella Intermedia 17 identifies Lipopolysaccharide Biosynthesis Pathway genes as potential drug targets." In 2016 International Conference on Bioinformatics and Systems Biology (BSB). IEEE, 2016. http://dx.doi.org/10.1109/bsb.2016.7552138.
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