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Dissertations / Theses on the topic 'Pregnenolone'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Sowa, Calan B. "The role of pregnenolone sulfate in modulating neuronal excitability." Thesis, Boston University, 2013. https://hdl.handle.net/2144/12230.

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Thesis (M.A.)--Boston University<br>The central nervous system operates through a tightly balanced relationship of excitatory and inhibitory synaptic transmission. Neurosteroids, synthesized de novo from cholesterol in the human brain as well as converted from precursors circulating in the blood, are proven modulators of this synaptic activity. Pregnenolone sulfate, one of the most abundant endogenous neurosteroids, is a negative modulator of the GABA receptor and positive modulator of NMDA and AMPA receptors, increasing the frequency of excitatory transmission in the brain. Here, we show that

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2

Darnaudéry, Muriel. "Sulfate de pregnenolone et mémoire : aspects comportementaux, neurochimiques et électroencéphalographiques." Bordeaux 2, 1998. http://www.theses.fr/1998BOR28608.

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Sano(Hamasaki), Mayumi. "Pregnenoloneは分裂期のcentriole engagementを制御する". 京都大学 (Kyoto University), 2015. http://hdl.handle.net/2433/195989.

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4

HU, ZHONG YI. "Neurosteroides : biosynthese et metabolisme de la pregnenolone dans les oligodendrocytes du cerveau de rat." Paris 6, 1989. http://www.theses.fr/1989PA066248.

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Les mitochondries d'oligodendrocytes de rats ages de 3 semaines transforment le #3h-cholesterol en #3h-pregnenolone(p), en #3h-pregn-5-ene-3 beta, 20 alpha-diol (20-oh-p). Les cultures primaires de cellules gliales de rats nouveaux-nes contiennent 60% d'oligodendrocytes, comme indique par la detection immunocytochimique au galactocerebroside, les oligodendrocytes contiennent aussi un antigene reconnu par des igg anti-p450scc, ces cultures primaires convertissent le #3h-mevolonolactone (mva) en #3h-cholesterol(c), en #3h-p et en #3h-20-oh-p. Les oligodendrocytes subissent un processus de differ

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5

Tomaselli, Giovanni. "Role of pregnenolone derivative AEF0117 on the regulation of CB1 signaling that mediates behavioral effects of THC." Thesis, Bordeaux, 2020. http://www.theses.fr/2020BORD0122.

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Le cannabis sativa est l'une des drogues les plus consommées dans le monde. Le THC, sa principale composante psychoactive, représente un facteur de risque de pathologies mentales, telles que le trouble de la consommation de cannabis, la dépendance et la psychose. Le THC a un effet biphasique, de fortes doses de THC provoquent une hypoactivité et une aversion, tandis que de faibles doses de THC provoquent une hyperactivité et une récompense. Le THC agit sur le récepteur aux cannabinoïdes de type 1 (CB1), dont la signalisation est biaisée avec différents transducteurs pouvant véhiculer une voie

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6

Urs, Aarti N. "Reciprocal binding of sphingosine and phosphatidic acid to steroidogenic factor 1 regulates the transcription of CYP17." Thesis, Available online, Georgia Institute of Technology, 2005, 2005. http://etd.gatech.edu/theses/available/etd-11212005-102620/.

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Thesis (M. S.)--Biology, Georgia Institute of Technology, 2006.<br>Donald Doyle, Committee Member ; Harish Radhakrishna, Committee Member ; Alfred Merrill, Committee Member ; Marion Sewer, Committee Chair Includes bibliographical references.

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7

Cox, Michele Margaret Freedman William McElligott James G. "The effects of a neurosteroid, pregnenolone sulfate, in the cerebellum on vestibulo-ocular reflex adaptation (VOR) in goldfish /." Philadelphia, Pa. : Drexel University, 2006. http://hdl.handle.net/1860/1119.

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8

Whang, Daniel. "Regulation of 7 alpha-hydroxylation of dehydroepiandrosterone and pregnenolone in human adipose stromal cells, mechanistic and expression studies." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq30833.pdf.

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9

Young, Jacques. "Les neurosteroides chez le rat et la souris : correlations fonctionnelles." Paris 11, 1996. http://www.theses.fr/1996PA11T006.

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10

Rouiller-Fabre, Virginie. "Production de pregnenolone et de progesterone par l'ovaire foetal de rat in vitro : ontogenese et regulation des activites enzymatiques." Paris 6, 1989. http://www.theses.fr/1989PA066436.

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Classiquement, il est admis depuis de nombreuses annees que l'ovaire ftal de mammifere ne possede pas d'activite staroidogene alors que, au meme moment, chez le male, une synthese de testosterone apparait. Le but de notre travail a ete de rechercher chez le rat les etapes limitantes de la steroidogenese ovarienne, au cours de la vie ftale. Nous avons etudie les deux etapes conduisant a la production de progesterone. Dans une premiere serie d'experiences, nous avons suivi, par dosage radioimmunologique, la production de pregnenolone et de progesterone d'ovaires ftaux de 18,5 j. P. C. Explantes

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11

Almarghalani, Daniyah Abduljalil. "Molecular Cloning, Expression, and Characterization of A Novel ZebrafishCytosolic Sulfotransferase, SULT5A1." University of Toledo Health Science Campus / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207.

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12

Presutti, Thais Rodrigues. "Aplicação da cromatografia a gás associada à espectrometria de massas em tandem no diagnóstico da deficiência de 3β-hidroxidesidrogenase." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-10052017-152745/.

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Pregnenolona (PREG) e 17-alfa-hidroxipregnenolona (17OHPREG) são dois esteroides produzidos pela glândula adrenal e precursores de vários hormônios esteroidais. A dosagem desses compostos tem aplicações clínicas, como o diagnóstico de doenças relacionadas aos corticoesteroides e mineralocorticóides e especialmente na avaliação da atividade da enzima 3-β-hidroxidesidrogenase que é decisiva no diagnóstico de um dos tipos de hiperplasia da glândula adrenal que causa defeitos severos na síntese de esteroides. Métodos cromatográficos associados à espectrometria de massas superaram a especifici

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13

Akwa, Yvette. "Neurosteroides : biosynthese et metabolisme de la pregnenolone et de la dehydroepiandrosterone dans le systeme nerveux central et le systeme nerveux peripherique chez le rat." Paris 6, 1993. http://www.theses.fr/1993PA066289.

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Les neurosteroides pregnenolone (preg) et dehydroepiandrosterone (dhea), precurseurs des hormones steroides dans les glandes endocrines classiques, ont ete mis en evidence dans le cerveau de rat a des taux inexpliques par leurs concentrations plasmatiques et les productions gonadiques et surrenaliennes. Leur 7alpha-hydroxylation est importante au niveau des microsomes cerebraux. Elle est observee principalement dans des cultures d'astrocytes de type 1 et d'oligodendrocytes. Son inhibition par l'oxyde de carbone suggere l'implication d'un cytochrome p450, probablement different des 7alpha-hydro

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14

Headlam, Madeleine Joyce. "Cytochrome P450scc (CYP11A1) : effects of glycerol and identification of the membrane binding domain." University of Western Australia. School of Biomedical and Chemical Sciences, 2004. http://theses.library.uwa.edu.au/adt-WU2004.0065.

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The first step in the synthesis of steroid hormones occurs in the mitochondria where cholesterol is converted to pregnenolone by cytochrome P450scc (CYP11A1). Cholesterol is insoluble in water and is supplied to the CYP11A1 directly from the inner mitochondrial membrane to which the enzyme is bound. The aim of this study was to characterise the interaction of bovine CYP11A1 with the phospholipid membrane. The effect of osmotic stress provided by glycerol on the spin-state, activity and degree of hydration of CYP11A1 was also investigated. Multiple sequence alignment of mitochondrial P450s reve

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15

Fazari, Benedetta [Verfasser], Joseph P. [Gutachter] Huston, and Bettina [Gutachter] Pause. "Effects of intranasal administration of Pregnenolone on Acetylcholine in regions of the rat brain assessed by in-vivo microdialysis / Benedetta Fazari ; Gutachter: Joseph P. Huston, Bettina Pause." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2019. http://d-nb.info/1200634012/34.

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16

Rahman, Mozibur. "Effects of neuroactive steroids on the recombinant GABAA receptor in Xenopus oocyte." Doctoral thesis, Umeå : Univ, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1112.

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17

Bresolin, Taise. "Expressão dos genes Receptor Pregnano X (PXR), Citocromo P4503A (CYP3A) e resistência a multidrogas 1(MDR1) em fígado de peixe-zebra (Danio rerio)." Florianópolis, SC, 2005. http://repositorio.ufsc.br/handle/123456789/102409.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas. Programa de Pós-Graduação em Biotecnologia.<br>Made available in DSpace on 2013-07-16T00:52:55Z (GMT). No. of bitstreams: 1 213393.pdf: 956920 bytes, checksum: a810d760f276b24af6058e83ddf60604 (MD5)<br>O receptor pregnano X (PXR) é um receptor nuclear envolvido na regulação transcricional de enzimas envolvidas no metabolismo de fármacos e também de proteínas transportadoras. Em mamíferos, vários xenobióticos induzem a expressão dos genes do citocromo P4503A (CYP3A) e de resistência a multidrogas 1 (

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18

López, Martínez José Eduardo, and Uribe Bernardo Antonio Frontana. "Oxidación Electroquímica de la Pregnenolona a Progesterona empleando el Sistema Br2/Tempo como Oxidante." Tesis de Licenciatura, Medicina-Quimica, 2014. http://ri.uaemex.mx/handle/123456789/14923.

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19

Hallé, François. "Conception, développement et synthèse de ligands du TSPO dans le but de traiter les maladies neurodégénératives." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAF054/document.

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Les neurostéroïdes sont des composés endogènes qui peuvent moduler la transmission synaptique et avoir un effet neuroprotecteur dans les maladies neurodégénératives. Les systèmes régulant leur biosynthèse ne sont pas connus mais la première étape de celle-ci peut être régulée par la protéine TSPO. Cette protéine mitochondriale facilite le transport du cholestérol vers l’intérieur de la mitochondrie pour y être métabolisé en prégnénolone. Ce stéroïde est le précurseur principal de la biosynthèse des neurostéroïdes et l’utilisation in vitro de ligands du TSPO permet d’augmenter sa sécrétion. Dan

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20

Sugunan, Kavitha. "Pregnenolone sulfate as a synaptic modulator." Thesis, 2016. https://hdl.handle.net/2144/14603.

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Pregnenolone (PREG), the precursor of all neurosteroids, is synthesized in the nervous system from cholesterol and recent clinical studies indicate that reduced cognitive symptoms of schizophrenia correlate with elevated serum levels of pregnenolone sulfate (PregS), its immediate sulfated metabolite. PregS fulfills most of the classical criteria for an endogenous modulator of excitatory synaptic transmission, including: presence in nervous tissue at physiologically relevant concentrations, potentiation of N-methyl-D-aspartate receptor (NMDAR) mediated synaptic activity, and a mechanism for its

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21

Weng, Jui-Hsia, and 翁瑞霞. "Pregnenolone Functions in Cytoskeleton, Cell Migration, and Zebrafish Development." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/01911685554203439390.

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博士<br>國立陽明大學<br>生化暨分子生物研究所<br>101<br>Pregnenolone (P5) is a neurosteroid that improves memory and neurological recovery. It is also required for zebrafish embryonic development. However, its mode of action is unclear. This thesis shows that P5 promotes cell migration and microtubule polymerization by binding to a microtubule-plus-end-tracking cytoplasmic linker protein 1 (CLIP-170). CLIP-170 was captured from zebrafish embryonic extract by a P5 photoaffinity probe conjugated to diaminobenzophenone and identified by LC-MS/MS analysis. P5 interacted with CLIP-170 at its coiled-coil domain and ch

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22

Cheng, Li-Kai, and 鄭吏凱. "Effects of Pregnenolone Sulfate on Learning and Memory in Normal and Epileptic Rats." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/96275824814177293459.

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碩士<br>國立成功大學<br>生理學研究所<br>88<br>The neurosteroid pregnenolone sulfate (PS) modulates neuronal excitability via a non-genomic mechanism. When administered intracerebroventricularly to experimental animals, PS also enhances post-training memory performance. Epilepsy is a disorder of brain function characterized by recurrent seizures that have a sudden onset and are evoked by over-activation of central neurons. During seizure, patients usually generate convulsive movement and sometimes lose consciousness. Memory deficits, particularly deficits of long-term memory, have often been reported in pati

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23

Chen, Shu-Cheng, and 陳淑貞. "Mechanisms Underlying the Effect of Pregnenolone Sulfate on Kainate and Glycine Receptor-Mediated Responses." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/96973042392617168494.

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24

Liu, Shu-Wei, and 劉書瑋. "Synthesis of CdTe Quantum Dots for Pregnenolone Binding Proteins Targeting and in Vivo Imaging." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/03177313902718758417.

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碩士<br>臺灣大學<br>化學研究所<br>98<br>The semiconductor nanocrystal quantum dots (QDs) are versatile fluorophores with unique photophysical properties, including narrow and size-dependent luminescence, broad absorption spectra, and high quantum yields. Bioconjugation of surface functionalized QDs is applied to biological imaging and cellular tracking.1 Embryonic cell movement is essential for morphogenesis and the establishment of body shapes. In previous research, we showed that the steroid hormone, pregnenolone, preserves microtubule abundance and promotes cell movement during epiboly.2 To visualize

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Smith, Conor C. "Non-canonical cell signaling actions of pregnenolone sulfate, a neurosteroid that increases intracellular calcium, activates creb phosphorylation and stimulates trafficking of NMDA receptors to the surface of neurons." Thesis, 2014. https://hdl.handle.net/2144/15222.

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Preclinical results support the use of N-methyl D-aspartate receptor (NMDAR) modulators for cognition enhancement therapeutics. Pregnenolone sulfate (PregS) is a neuroactive steroid derived from cholesterol that augments long term potentiation (LTP) in hippocampal slices and improves memory performance in rats and mice. At micromolar concentrations, PregS is a subtype selective positive allosteric modulator of NMDARs at NR2A and NR2B containing receptors, and at concentrations ranging from pM - nM induces NMDAR-dependent dopamine release in the striatum and from striatal synaptosomes. In this

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Chen, Shu-Cheng, and 陳淑貞. "Mechanisms Underlying Inhibition of the Capsaicin Receptor-Mediated Responses by Dehydroepiandrosterone and Pregnenolone Sulfate in Rats." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/24529793457773795573.

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博士<br>國立成功大學<br>基礎醫學研究所<br>93<br>Previous studies have shown that neurosteroids dehydroepiandrosterone (5-androsten-3b-ol-17-one; DHEA) and pregnenolone sulfate (5-pregnen-3b-ol-20-one sulfate; PS) modulate amino acid receptor-mediated responses in brain. Compared to the extensive studies of DHEA and PS effect on amino acid receptors, relatively little is known of interaction of DHEA and PS with the capsaicin receptor. In the present study, we investigated the effects of DHEA and PS on the capsaicin receptor-mediated current in acutely dissociated rat dorsal root ganglion neurons using the who

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Chen, Chien-Han, and 陳建漢. "Development of Credible Molecular Probes to Identify Pregnenolone-Binding Proteins and Site-Selectively Alkylate G-Quadruplex DNA." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/86330457034233765253.

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博士<br>國立臺灣大學<br>化學研究所<br>104<br>The work presented here consists of two parts: Section I describes that pregnenolone (P5) was equipped with benzophenone photoreactive group and biotin tag at C7 position in ether linkage to explore P5-binding proteins in the stage of embryonic development of the zebrafish. Various spacer lengths and orientations of P5-photoaffinity probes had been employed to investigate the influences on the activity of in vitro tubulin polymerization. With the preservation of the biological functions as P5, P5-NBPN was used to label P5-binding proteins from zebrafish embryo l

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CHUANG, CHING-YU, and 莊靖渝. "Investigating the Neuroprotective Effects of Detromethorphan and Pregnenolone Sulfate in Alzheimer’s Disease Rat Model Using PET Imaging Techenique." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/91197853090937374392.

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碩士<br>國防醫學院<br>生物及解剖學研究所<br>104<br>Alzheimer’s disease (AD) is a progress neurodegenerative disorder that associated with the defects of cognition and memory. Recent studies showed that insulin resistant in the brain may play an important role in the etiopathogenesis of the sporadic AD (sAD). In this study, the SD rat with streptozotocin intracerebroventricular (STZ-icv) treatment was used to be a sAD animal model. The open field test (OFT) and radial arm maze (RAM) behavior test were carried out to evaluate the cognitive and memory deficits in STZ-icv rat. Moreover, the [18F]FDG and [18F]T807

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29

Huang, Tzu-Chiao, and 黃子樵. "Synthesis and Application of Affinity-Based Probes: Identification of Pregnenolone Binding Proteins and Study of Telomere-Directed DNA Alkylating Agents." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/21823495243268046594.

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碩士<br>國立臺灣大學<br>化學研究所<br>100<br>Affinity-based probes (AfBPs) have been developed recently by several research groups. Both of Cravatt and Yao groups independently reported the first AfBPs for metalloproteases. AfBPs achieves labelling by binding at a specific site of a protein followed by a non-specific covalent bond-forming event. The seminal works of AfBPs research enlighten us to design and synthesize probes for targeting different biomolecules of interest. 　　Embryonic cell movement is essential for morphogenesis and establishment of body shape. In the previous research reported by our gro

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30

Chen, Chao-wei, та 陳兆偉. "Mechanisms Underlying the Effects of Pregnenolone Sulfate and 17β-Estradiol on Capsaicin-Induced Thermal Hyperalgesia and Nocifensive Responses in Male Rats". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/69807462657241770779.

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碩士<br>國立成功大學<br>生理學研究所<br>95<br>The capsaicin receptor is expressed predominantly by sensory neurons and has been implicated in nociception and inflammatory thermal hyperalgesia. Previous studies from our laboratory have shown that the neurosteroid pregnenolone sulfate (PS) inhibits, but the female sex steroid 17β-estradiol (E2) potentiates, both the capsaicin receptor-mediated current in male rat dorsal root ganglion neurons and the capsaicin receptor-mediated nocifensive response in male rats. However, it is unclear whether PS and E2 also have modulatory effects on capsaicin-induced thermal

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Lin, Shiao-Han, and 林筱涵. "Ⅰ Synthesis and Application of Photoaffinity Probes : Identification of Pregnenolone Binding Proteins and Study of G-Quadruplex Helicase Ⅱ Synthesis of Telomere-Directed DNA Alkylating Agents Based on Prodrug Concept." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/85135478497642171722.

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碩士<br>國立臺灣大學<br>化學研究所<br>101<br>The development of proteomics has opened up new horizons for the research of many diseases and drugs invention. Meanwhile, the related technology of affinity-based probes (AfBPs) provides more direct evidence about the mediation and regulation of protein in physiological condition. The actions of AfBPs are mainly based on molecular recognition, with the introducing of photoreactive groups (PGs) to achieve the specific covalent modification on protein. With the directing of different substrate, allowing scientists for the corresponding binding protein studies to

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Mahler, Philip. "Longitudinalverlauf der Pregnenolon und 17-OH-Pregnenolon bei Früh- und Neugeborenen in der Neonatalperiode." 2004. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=014189046&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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Silva, Vitor Rafael Miranda da. "Revisão sistemática sobre a influência dos níveis de pregnenolona e sulfato de pregnenolona na doença de Alzheimer." Master's thesis, 2021. http://hdl.handle.net/10400.6/11724.

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O presente trabalho está dividido em dois capítulos que descrevem a minha experiência profissionalizante para a obtenção de grau de Mestre em Ciências Farmacêuticas, apresentando uma componente de investigação, bem como uma componente de estágio realizado em Farmácia Comunitária. O primeiro capítulo é composto por uma revisão sistemática de artigos em que foram comparados os níveis de pregnenolona e sulfato de pregnenolona, em amostras biológicas de doentes com doença de Alzheimer e grupos controlo sem apresentação de demência. A doença de Alzheimer é a causa mais comum de demência globalmente

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Wagener, Juliane. "Induktion der Cytochrom-P450-Isoformen CYP3A1/2 durch Dexamethason (Dexa) und Pregnenolon-16alpha-Carbonitril (PCN) in Leberpräzisionsschnitten der Ratte /." 2004. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=014193034&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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