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Journal articles on the topic 'Porcine'

Author: Grafiati

Published: 4 June 2021

Last updated: 21 February 2023

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1

Zemenu Adiss, Getnet. "Porcine Circovirus: Historical Outlooks and Non-Porcine Victims." Open Access Journal of Veterinary Science & Research 5, no. 1 (2020): 1–5. http://dx.doi.org/10.23880/oajvsr-16000191.

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Porcine circovirus is an important viral species in the genus circovirus. It causes an immerse economic losses in the piggery industry. According to the retrospective studies, PCV2 has circulated before its acclaimed detection from samples taken as of the first outbreak in Canada. A bit far on in time, it has been reported in Europe, United States and Asian countries. The disease is endemic in most pig producing countries. Since then phylogeny studies supported for the immergence of various new Porcine circoviruses variants and genotypes. In addition to its natural reservoirs (wild and feral pigs), it also inhibits calves, goats, canines and mice. Some insects like mosquitoes are also the potential carrier of PCV2 even let it for cross species transmission. Hence those proper prevention measures of the mechanical carrier vectors of the disease should be noted together with the need of efficient vaccine against the pathogenic porcine circoviruses types.

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2

Stafford, K., Y. Stafford, D. Paton, and P. Gamble. "Anticorps de quelques maladies du porc dans les élevages commerciaux du centre de la Zambie." Revue d’élevage et de médecine vétérinaire des pays tropicaux 45, no. 3-4 (March 1, 1992): 229–30. http://dx.doi.org/10.19182/remvt.8907.

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Des échantillons de sang ont été prélevés sur 121 truies (jeunes et adultes) provenant de 7 porcheries commerciales situées autour de Lusaka (Zambie). Ils ont été reconnus négatifs pour la maladie d'Aujeszky, la gastroentérite contagieuse, la grippe porcine, la peste porcine classique et la brucellose. Soixante dix huit porcs provenant de cinq fermes ont réagi positivement au parvovirus porcin. Dix huit sérums se sont révélés positifs à Leptospira celledoni.

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3

Barrow, Rachel T., John F. Healey, David Gailani, Dorothea Scandella, and Pete Lollar. "Reduction of the antigenicity of factor VIII toward complex inhibitory antibody plasmas using multiply-substituted hybrid human/porcine factor VIII molecules." Blood 95, no. 2 (January 15, 2000): 564–68. http://dx.doi.org/10.1182/blood.v95.2.564.

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Factor VIII (fVIII) circulates as a heavy chain/light chain (A1-A2-B/ap-A3-C1-C2) heterodimer. The 41-residue light chain activation peptide, ap, is cleaved from fVIII during proteolytic activation by thrombin or factor Xa. We constructed 7 active recombinant hybrid B-domainless human/porcine fVIII molecules that contained combinations of porcine sequence replacements within the A2, ap-A3, and C2 domains. The cross-reactivity of 23 high-titer inhibitory antibodies between human fVIII and the hybrids was inversely related to the degree of porcine substitution. In all plasmas, the substitution of all 3 regions yielded cross-reactivities that were not significantly different from those of porcine fVIII. To differentiate between inhibitor binding to the ap region and the A3 domain, we constructed 2 additional hybrids that contained porcine A2 and C2 domain substitutions and either porcine A3 or porcineap substitutions. The porcine ap segment was less antigenic than the human ap segment in several plasmas that had activity against the ap-A3 region. This indicates that some inhibitor plasmas contain antibodies directed against the fVIIIap segment in addition to A2, A3, and C2 domain epitopes identified in previous studies. Substitution of porcine sequences within the A2, A3, C2, and ap regions of human fVIII is necessary and sufficient to achieve a maximal reduction in antigenicity relative to porcine fVIII with respect to most inhibitory antibody plasmas.

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4

Fibrianto, Yuda Heru. "Development of Intergeneric Canine Embryo Using Bovine and Porcine Oocyte as Cytoplasm Recipient." Indonesian Journal of Biotechnology 10, no. 1 (October 13, 2015): 801. http://dx.doi.org/10.22146/ijbiotech.16366.

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This study wa conducted to increase the efficiency of canine embryo production by intergeneric somatic cell nuclear transfer (SCNT) technology. The effect of oocyte recipient for development of intergeneric canine somatic cell cloning embryos were examined. Bovine and porcine cumulus oocyte complexes (COCs) were collected from slaughterhouse ovaries and matured in TCM-199 medium depend on species. Adult dog fibroblasts collected from 3.5 years old Afghandhound dog, and cell between passage 1 and passage 10 used for intergeneric somatic cell cloning using bovine and porcin oocytes as oocyte cytolplasm donor. The result showed that oocytes from bovine and porcine can de-differentiated canine nucleus and no different between bovine and porcine oocyte in fusion and embryo development in vitro. Canine intergeneric cloned embryos developed to morula stages in vitro.

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5

Ramos-Clamont, M. G., and L. Vázquez-Moreno. "FOAMING PROPERTIES OF PORCINE SERUM AND PORCINE SERUM ALBUMIN PROPIEDADES ESPUMANTES DEL SUERO Y ALBÚMINA PORCINA." Ciencia y Tecnologia Alimentaria 5, no. 2 (July 2006): 105–11. http://dx.doi.org/10.1080/11358120609487679.

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6

Marchot, P., A. Vermeylen, Guy Hendrickx, and Philippe Leroy. "Observation de sclérectasie dans un élevage de porc à Sotouboua, Togo." Revue d’élevage et de médecine vétérinaire des pays tropicaux 45, no. 3-4 (March 1, 1992): 227–28. http://dx.doi.org/10.19182/remvt.8906.

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Des cas de sclérectasie ont été décrits dans un élevage porcin à Sotouboua, Togo. Le virus de la peste porcine pourrait en être l'origine, la consanguinité étant un facteur prédisposant. Les éleveurs doivent rester vigilants.

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7

Zheng, LL, XH Jin, FS Wei, CQ Wang, HY Chen, YB Wang, and ZY Wei. "Simultaneous detection of porcine pseudorabies virus, porcine parvovirus and porcine circovirus type 2 by multiplex real-time PCR and amplicon melting curve analysis using SYBR Green I." Veterinární Medicína 63, No. 8 (August 20, 2018): 358–66. http://dx.doi.org/10.17221/3/2018-vetmed.

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Porcine parvovirus, porcine pseudorabies virus and porcine circovirus type 2 can cause reproductive failure in pigs, and swine are often simultaneously infected by combinations of the three viruses. We here report the development of a SYBR Green I-based multiplex real time PCR assay for simultaneous detection of porcine parvovirus, porcine pseudorabies virus and porcine circovirus type 2. Three pairs of specific primers were designed for the porcine parvovirus-VP2, porcine pseudorabies virus-gH and porcine circovirus type 2-ORF2 genes. Viral genomes were identified based on their distinctive melting temperatures in singleplex PCR reactions. The melting temperature was 74.5 °C for the 313 bp amplicon of porcine parvovirus-VP2 gene, 87.5 °C for the 355 bp amplicon of porcine pseudorabies virus-gH gene and 80.5 °C for the 171 bp amplicon of the porcine circovirus type 2-ORF2 gene, respectively. The detection limit of the method ranged from 0.01–0.03 TCID<sub>50</sub>/ml for the three viruses. In addition, porcine parvovirus, porcine pseudorabies virus and porcine circovirus type 2 viral loads were measured in 100 field samples, and the result showed that the concordance between real-time PCR and conventional PCR was 60.42%. The sensitivity and specificity of real-time PCR were 100% and 100%, while those of conventional PCR were 40.83% and 72.22%, respectively.

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8

MORVAN, Hervé. "DOMINANTES EN PATHOLOGIE PORCINE : la filière porcine." Bulletin de l'Académie vétérinaire de France, no. 1 (2011): 43. http://dx.doi.org/10.4267/2042/48068.

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9

Ramsay, T. G. "Porcine leptin inhibits lipogenesis in porcine adipocytes1,2." Journal of Animal Science 81, no. 12 (December 1, 2003): 3008–17. http://dx.doi.org/10.2527/2003.81123008x.

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10

Petrová, I., M. Sedmíková, E. Chmelíková, D. Švestková, and R. Rajmon. "In vitro aging of porcine oocytes." Czech Journal of Animal Science 49, No. 3 (December 12, 2011): 93–98. http://dx.doi.org/10.17221/4285-cjas.

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Porcine oocytes matured in vitro develop in various ways if they are further cultivated. In our studies these oocytes were cultivated for 1 to 5 days (in vitro aging). During the 1st day of aging, most of them remained at the stage of metaphase II (98%). Then many oocytes underwent the spontaneous parthenogenetic activation. The portion of activated oocytes reached its peak after 2 or 3 days of aging in vitro (39 or 45%). The portion of fragmented oocytes peaked at the same time (28%). During subsequent aging in vitro (i.e. day 4 or 5 of aging), the portion of lysed oocytes significantly increased (30 or 37%). The highest portion of spontaneously activated parthenogenetic embryos at a pronuclear stage (35%) was observed during the 2nd day of aging in vitro. These pronuclear embryos had mainly one polar body with two pronuclei (47% of all pronuclear embryos) or two polar bodies with one pronucleus (38% of all pronuclear embryos). During the 3rd and 5th day of in vitro aging, there was a significant increase in the portion of parthenogenetic embryos cleaved to the 2-cell or 3-cell stage. When considering the prolonged in vitro culture of porcine oocyte, only the first day of aging should be taken into account, since beyond this time significant changes, i.e. parthenogenesis, fragmentation or lysis, occurred in oocytes under in vitro conditions.  

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11

Carr, John, Mark Howells, and William Hersey. "Porcine circoviruses." Livestock 26, no. 3 (May 2, 2021): 144–49. http://dx.doi.org/10.12968/live.2021.26.3.144.

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Porcine circoviruses have become an integral part of the pig production landscape. They are an evolving pathogen whose impact spans non-pathogenic to association with some of the most serious and far-reaching pathological conditions of pigs.

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12

Ellis, J. "Porcine Circovirus." Veterinary Pathology 51, no. 2 (February 25, 2014): 315–27. http://dx.doi.org/10.1177/0300985814521245.

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13

Scott, A., M. Chaplin, M. Stack, and L. Lund. "Porcine torovirus?" Veterinary Record 120, no. 24 (June 13, 1987): 583. http://dx.doi.org/10.1136/vr.120.24.583-a.

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14

Lorenti, A., M. Barbich, A. Schenone, A. Guinle, N. Chamoles, P. Sorroche, A. Gonzalez, A. Fustiniana, and P. Argibay. "PORCINE HEPATOCYTES." ASAIO Journal 47, no. 2 (March 2001): 164. http://dx.doi.org/10.1097/00002480-200103000-00251.

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15

Middleton, Sheldon. "Porcine Ophthalmology." Veterinary Clinics of North America: Food Animal Practice 26, no. 3 (November 2010): 557–72. http://dx.doi.org/10.1016/j.cvfa.2010.09.002.

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16

Yamakoshi, Y., T. Tanabe, M. Fukae, and M. Shimizu. "Porcine amelogenins." Calcified Tissue International 54, no. 1 (January 1994): 69–75. http://dx.doi.org/10.1007/bf00316293.

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17

Murtaugh, Michael P. "Porcine cytokines." Veterinary Immunology and Immunopathology 43, no. 1-3 (October 1994): 37–44. http://dx.doi.org/10.1016/0165-2427(94)90118-x.

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18

Sakamoto, K., E. Hatakeyama, T. Kenmochi, K. Yamada, C. Iwashita, T. Asano, H. Kashiwabara, and T. Yokoyama. "Improvement of Porcine Islet Culture With Porcine Serum." Transplantation Proceedings 30, no. 2 (March 1998): 391–92. http://dx.doi.org/10.1016/s0041-1345(97)01325-0.

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19

Waldman, J., T. Vogel, C. Burlak, P. Friend, and M. Rees. "Blocking Porcine Sialoadhesin Improves Extracorporeal Porcine Liver Xenoperfusion." Transplantation Journal 94, no. 10S (November 2012): 568. http://dx.doi.org/10.1097/00007890-201211271-01097.

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20

Takefman, Daniel M., Susan Wong, Thomas Maudru, Keith Peden, and Carolyn A. Wilson. "Detection and Characterization of Porcine Endogenous Retrovirus in Porcine Plasma and Porcine Factor VIII." Journal of Virology 75, no. 10 (May 15, 2001): 4551–57. http://dx.doi.org/10.1128/jvi.75.10.4551-4557.2001.

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ABSTRACT The pig genome contains porcine endogenous retroviruses (PERVs) capable of infecting human cells. Detection of infectious retrovirus in porcine peripheral blood mononuclear cells and endothelial cells suggested to us that pig plasma is likely to contain PERV. Both PERV env sequences and viral reverse transcriptase (RT) activity were detected in all plasma samples isolated from four NIH minipigs. To detect infectious virus from plasma, we performed a culture assay using three cell lines of feline, swine, and human origin that had previously been shown to be permissive for PERV. Infectious virus was successfully cultured from all four NIH minipig plasmas on the swine cell line ST-IOWA. Using RT-PCR with env-specific primers, we could detect expression of PERV class C envelope in the supernatant of ST-IOWA cells that had been exposed to each pig plasma. We next examined a pig plasma derivative, Hyate:C (porcine factor VIII), and found evidence of PERV particles, since all six lots examined were positive for PERV RNA and RT activity. However, infectious virus could not be detected in clinical lots of Hyate:C, suggesting that the manufacturing process might reduce the load of infectious virus to levels below detectable limits of the assay. Detection of infectious virus in porcine plasma confirms and extends the previous findings that certain porcine cells express PERV when manipulated in vitro and clearly demonstrates that there are porcine cells that express infectious PERV constitutively in vivo.

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21

BIDANEL, J. P., P. LE ROY, L. OLLIVIER, M. BONNEAU, P. CHARDON, J. M. ELSEN, J. GELLIN, and D. MILAN. "Etablissement et utilisation de la carte génétique porcine." INRAE Productions Animales 9, no. 4 (August 17, 1996): 299–310. http://dx.doi.org/10.20870/productions-animales.1996.9.4.4063.

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Des programmes de cartographie des génomes des principales espèces animales d’intérêt économique se sont développés depuis quelques années. Cet article fait le point sur l’état d’avancement et les perspectives d’utilisation de la carte génétique porcine. Après une présentation des principes généraux de la cartographie génétique, les résultats des principaux programmes de cartographie du génome porcin sont présentés. Différentes perspectives d’utilisation des cartes génétiques sont ensuite évoquées. Les possibilités et les modalités d’utilisation des marqueurs génétiques pour la détection et la localisation de locus à effets quantitatifs (QTL) sont présentées. L’intérêt de la cartographie comparée pour l’identification des QTL est illustré à partir de l’exemple du système majeur d’histocompatibilité. Quelques perspectives d’utilisation de l’information apportée par les marqueurs dans les programmes d’amélioration génétique (introgression d’un gène, sélection assistée par marqueurs) sont ensuite évoquées. Enfin, les principaux programmes de recherche utilisant la carte génétique porcine actuellement en cours à l’INRA (programmes de localisation de gènes RN et MU, programme de recherche des QTL responsables de la variabilité génétique entre les races Meishan et Large White) sont présentés.

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22

BIDANEL, J. P., P. LE ROY, L. OLLIVIER, M. BONNEAU, P. CHARDON, J. M. ELSEN, J. GELLIN, and D. MILAN. "Etablissement et utilisation de la carte génétique porcine." INRAE Productions Animales 9, no. 4 (August 20, 1996): 299–310. http://dx.doi.org/10.20870/productions-animales.1996.9.4.4070.

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Des programmes de cartographie des génomes des principales espèces animales d’intérêt économique se sont développés depuis quelques années. Cet article fait le point sur l’état d’avancement et les perspectives d’utilisation de la carte génétique porcine. Après une présentation des principes généraux de la cartographie génétique, les résultats des principaux programmes de cartographie du génome porcin sont présentés. Différentes perspectives d’utilisation des cartes génétiques sont ensuite évoquées. Les possibilités et les modalités d’utilisation des marqueurs génétiques pour la détection et la localisation de locus à effets quantitatifs (QTL) sont présentées. L’intérêt de la cartographie comparée pour l’identification des QTL est illustré à partir de l’exemple du système majeur d’histocompatibilité. Quelques perspectives d’utilisation de l’information apportée par les marqueurs dans les programmes d’amélioration génétique (introgression d’un gène, sélection assistée par marqueurs) sont ensuite évoquées. Enfin, les principaux programmes de recherche utilisant la carte génétique porcine actuellement en cours à l’INRA (programmes de localisation de gènes RN et MU, programme de recherche des QTL responsables de la variabilité génétique entre les races Meishan et Large White) sont présentés.

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23

Uribe, Alberto Orrego, and Julialba Angel Osorio. "Impacto económico de la leptospirosis en dos explotaciones porcinas de la zona cafetera colombiana." Corpoica Ciencia y Tecnología Agropecuaria 2, no. 1 (July 31, 1997): 27. http://dx.doi.org/10.21930/rcta.vol2_num1_art:159.

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<p>La leptospirosis es una enfermedad infecciosa, altamente contagiosa y ubicua, que afecta severamente la porcicultura; no obstante, su impacto económico no había sido estimado en los sistemas de producción del país. El conocimiento del impacto económico de una enfermedad permite estimar su importancia respecto de otras condiciones patológicas y escoger las mejores alternativas de prevención y control. El presente trabajo se llevó a cabo para determinar el impacto económico de la leptospirosis en el desempeño reproductivo de cerdas de cría; además, para proponer soluciones correctivas, sostenibles y eficientes frente a la ocurrencia de la enfermedad. Las evaluaciones se realizaron en dos explotaciones de la zona cafetera colombiana, identificadas como “A” y “B”, las cuales pose en un inventario promedio de 189 y 674 hembras de cría, respectivamente. Las explotaciones porcinas fueron monitoreadas mediante el programa PígChamp<sup>®</sup> durante los períodos preepizoótico, epizoótico y postepizoótico. Los costos de producción de cada granja porcina se calcularon por el sistema de costos fijos y variables, mientras el impacto económico se estimó mediante un procedimiento adaptado por los autores. Al estimar la pérdida total (en pesos de 1995, US$ 1= $1.ooo) ésta fue de 21 millones de peso s colombianos ($) en la explotación porcina A, y de $44 millones en la B. El notable incremento del parámetro "días/cerda no productivos" hizo evidente la magnitud de las pérdidas económicas; éstas ascendieron a $11,5 millones (54,1% del total) en la granja porcina A, y a$ 19 millones (42,5% del total) en la B. La “pérdida promedio por cerda”, fue de $117.855 en la explotación A, y de $67.818 en la B. Se recomienda la vacunación y otras medidas complementarias de control, así como la producción de inmunógenos moleculares específicos.</p><p> </p><p><strong>Economic impact of porcine leptospirosis at two farms of the Colombian coffee growers área</strong></p><p>Porcine leptospirosis is an infectious, highly contagious and ubiquitous disease, severely affecting the porcine industry; yet, its economic impact had not been estimated. Knowledge about the economical impact of a malady allows to estimate its relevance in relation to other pathologic conditions and the selection of the best prevention and control alternatives. This work was carried out in order to estimate the economic impact of leptospirosis on breeding sows and to propase efficient, sustainable and corrective solutions. The evaluations were carried out at two farms of the Colombian coffee growers area, identified as “A” and “B”, with a final average inventory of 189 and 674 sows, respectively. The porcine farms were monitored using PigChamp™ Program during the preepizootic, the epizootic and the postepizootic periods. The costs of production of each pig unit were calculated by the Fixed and Variable Costs System; the economical impact was estimated using a modified procedure. The total loss (estimated as US$1=$1.ooo in 1995) at farm A was US $21,000, and US $44,000 at farm B. The critical enlargement on “sows non productive days” parameter, makes evident the amount of economical loss, being of US $11,500 (54.1% of the total) at farm A, and US $19,000 (42.5%) at farm B. The “average loss per sow” reached US $117.885 at farm A, and US $67.8 at farm B. The vaccination against leptospirosis, the production of molecular immunogens, as well as other complementary control measures, are recommended.</p>

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24

Koshihara, K., J. Qian, P. Lollar, and LW Hoyer. "Immunoblot cross-reactivity of factor VIII inhibitors with porcine factor VIII." Blood 86, no. 6 (September 15, 1995): 2183–90. http://dx.doi.org/10.1182/blood.v86.6.2183.bloodjournal8662183.

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Porcine factor VIII has been used successfully to treat factor VIII inhibitor patients whose plasmas have minimal cross-reactivity to porcine factor VIII. However, some inhibitor plasmas do inhibit porcine factor VIII, and the extent of procoagulant inhibition often increases after treatment with porcine factor VIII. Because there is no information about the porcine factor VIII epitopes with which these antibodies react, we have compared the immunoblot and enzyme-linked immunosorbent assay (ELISA) reactivities with porcine and human factor VIII for 20 inhibitor plasmas (11 from hemophilia A patients and 9 autoantibodies). Immunoblots identified binding to porcine factor VIII for only 2 of the 12 plasmas from patients who had not received porcine factor VIII, but this reactivity could not be predicted from the inhibitor titer to porcine factor VIII. Immunoblot reactivity with porcine factor VIII was detected for 7 of 8 inhibitor plasmas from patients who had been previously treated with porcine factor VIII, and the strength of this reactivity was generally related to the inhibitor titer. Of the 5 plasmas that were immunoblot positive with the porcine factor VIII A2 domain, 4 had inhibitor titers greater than 45 Bethesda units when tested with porcine factor VIII, whereas only 1 of 15 of the other plasmas had this level of inhibitor activity with porcine factor VIII. In contrast, immunoblot reactivity to the porcine factor VIII A1 domain did not correlate with the antiporcine VIII inhibitor titer. We also determined the effect of preincubation with human or porcine factor VIII on immunoblot reactivity. In one case, immunoblot reactivity with porcine factor VIII was absorbed with porcine, but not human, factor VIII, which is consistent with antibody formation after treatment with porcine factor VIII. In no cases did human factor VIII reduce the reactivity of inhibitor plasmas with the porcine A1 domain, suggesting that these antibodies are directed at unique porcine factor VIII determinants. The reactivity to porcine A2 in 2 plasmas probably represented cross-reactivity of similar A2 determinants, because it was absorbed by both human and porcine factor VIII. Although the ELISA assays with porcine factor VIII detected antibodies in some plasmas that could not be identified by inhibitor assay or immunoblot, the level of ELISA reactivity was generally consistent with the titers of the other assays.

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25

Malik, Anna, István Tóth, and Béla Nagy. "Colonisation of conventional weaned pigs by enteropathogenic Escherichia coli (EPEC) and its hazard potential for human health." Acta Veterinaria Hungarica 60, no. 3 (September 1, 2012): 297–307. http://dx.doi.org/10.1556/avet.2012.025.

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EnteropathogenicEscherichia coli(EPEC) bacteria frequently cause severe enteric diseases primarily in children and in young rabbits. Their pathogenicity for pigs has been tested by oral infection of colostrum-deprived newborn, and of severely immunosuppressed weaned pigs, but colonisation of conventional weaned pigs by porcine EPEC has not been experimentally studied. EPEC show similarities to enterohaemorrhagicE. coli(EHEC) additionally carrying shiga toxin genes integrated into the chromosome by lambdoid phages. We have demonstrated earlier that the porcine EPEC prototype strain P86-1390 (O45) could be transducedin vivo(in ligated loops of weaned pigs), by Stx2 phage derived from a human EHEC. Thus, the ability of this porcine EPEC strain to colonise conventional weaned pigs under farming conditions became a question of relevance to human health. To clarify this question, four intragastric infection experiments were performed on a total of 95 conventional weaned pigs. The EPEC P86-1390 and other well-characterised porcine EPEC strains were applied to 54 pigs, leaving 41 weaned pigs as negative controls. In three experiments moderate predispositions were applied: coinfections with enterotoxigenicE. coli(ETEC) or with low-virulence TGE coronavirus, application of fumonisin B1with a normal therapeutic dose of dexamethasone, and the increase of soybean protein concentration in the feed. A total of 41 weaned pigs served as negative controls inoculated with a commensal porcineE. coli. Housing conditions simulated the farm environment. As an overall result, ileal segments of 18.5% of infected pigs were shown to be colonised by EPEC, while no EPEC were detected in the ilea of controls. Among predisposing factors occurring on farms, feed protein content increased by 20% (26.3% crude protein, provided by 48% soybean meal) seemed to enhance EPEC colonisation and resulted in the mobilisation of spontaneous latent EPEC/ETEC infection. The results indicate that under normal farm conditions porcine EPEC may colonise conventional weaned pigs by inducing ileal attaching effacing (AE) lesions with reasonable frequency, without clinical signs. The results also suggest that conventional weaned pigs may represent undetected reservoirs of porcine EPEC, potentially giving rise to the emergence of new types of EHEC due to natural transduction by Stx phages.

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26

Ellis, John, Maria Spinato, Choon Yong, Keith West, Francis McNeilly, Brian Meehan, Seamus Kennedy, Edward Clark, Steven Krakowka, and Gordon Allan. "Porcine Circovirus 2–Associated Disease in Eurasian Wild Boar." Journal of Veterinary Diagnostic Investigation 15, no. 4 (July 2003): 364–68. http://dx.doi.org/10.1177/104063870301500411.

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Porcine circovirus 2 (PCV2) was first identified in high-health herds of domestic swine and was associated with a debilitating disease called postweaning multisystemic wasting syndrome (PMWS). Most subsequent studies have indicated that PCV2 infects only swine but there is little information on porcids other than improved breeds of domestic swine. Multisystemic disease was reported in a group of Eurasian wild boars raised under free-range conditions. Affected young pigs had pneumonia and enteritis and were cachectic. Porcine circovirus 2 was identified in affected tissue by immunohistochemistry and in situ hybridization, and a PCV2-like virus was isolated from pooled organs. The open reading frame (ORF2) of the isolated PCV2 had a 98.7% homology with the ORF2 of a reference PCV2 isolate. These diagnostic data indicate that PCV2 can infect and cause disease in Sus scrofa subspecies other than domestic swine.

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27

MARTINAT-BOTTE, F., A. M. PLAT, and P. GUILLOUET. "Biotechnologies de la reproduction porcine : des techniques de routine aux méthodes en émergence." INRAE Productions Animales 22, no. 2 (April 15, 2009): 97–116. http://dx.doi.org/10.20870/productions-animales.2009.22.2.3338.

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Les techniques d’assistance à la reproduction et les biotechnologies qui en sont dérivées sont en plein développement dans l’espèce porcine. L’objectif de cet article de synthèse est de décrire les techniques qui sont d’usage courant en reproduction porcine (insémination de semence conservée à l’état liquide, synchronisation des oestrus, échographie...), celles dont l’avancement permet d’envisager des applications dans un avenir proche (insémination de semence conservée congelée, production et cryoconservation des embryons, transfert chirurgical d’embryons) et celles qui sont en cours de développement et permettront d’ouvrir dans un avenir plus lointain de nouvelles perspectives pour l’élevage porcin (sexage de la semence, transfert non chirurgical d’embryons). L’ensemble de ces techniques, y compris celles qui sont d’ores et déjà d’utilisation courante, connaissent des progrès rapides en termes d’amélioration des résultats et nous essaierons de décrire les principales pistes poursuivies dans ce sens.

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28

Anh, Khoa, and K. Wimmers. "Assignment for genes encoding the terminal complement components to porcine chromosome." Biotehnologija u stocarstvu 30, no. 3 (2014): 407–14. http://dx.doi.org/10.2298/bah1403407a.

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One of the major goals of the porcine genome projects is building a physical map. To assign the porcine genes encoding the complement components C6, C7, C8 and C9 to porcine chromosomes, we used a porcine 7000Rad Radiation Hybrid panel (IMpRH) containing 118 clones provided by INRA-University of Minnesota. It resulted in assignment of the porcine C6, C7 and C9 genes to chromosome 16q1.4, the porcine C8A and C8B genes to chromosome 6q3.1-q3.5 as well as the porcine C8G gene lonely to chromosome 1q2.13.

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29

Zhu, Xinyu, Shudan Liu, Xunlei Wang, Zhaochen Luo, Yuejun Shi, Dang Wang, Guiqing Peng, Huanchun Chen, Liurong Fang, and Shaobo Xiao. "Contribution of porcine aminopeptidase N to porcine deltacoronavirus infection." Emerging Microbes & Infections 7, no. 1 (April 11, 2018): 1–13. http://dx.doi.org/10.1038/s41426-018-0068-3.

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30

Gillespie, J., T. Opriessnig, X. J. Meng, K. Pelzer, and V. Buechner-Maxwell. "Porcine Circovirus Type 2 and Porcine Circovirus-Associated Disease." Journal of Veterinary Internal Medicine 23, no. 6 (November 2009): 1151–63. http://dx.doi.org/10.1111/j.1939-1676.2009.0389.x.

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31

Liu, Xiao, Zhiwen Xu, Ling Zhu, Shan Liao, and Wanzhu Guo. "Transcriptome Analysis of Porcine Thymus following Porcine Cytomegalovirus Infection." PLoS ONE 9, no. 11 (November 25, 2014): e113921. http://dx.doi.org/10.1371/journal.pone.0113921.

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32

Goldfarb, Roy D., R. Phillip Dellinger, and Joseph E. Parrillo. "PORCINE MODELS OF SEVERE SEPSIS: EMPHASIS ON PORCINE PERITONITIS." Shock 24, Supplement 1 (December 2005): 75–81. http://dx.doi.org/10.1097/01.shk.0000191337.01036.b7.

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Jerry, D. J., L. C. Griel, J. F. Kavanaugh, and R. S. Kensinger. "Binding and bioactivity of ovine and porcine prolactins in porcine mammary tissue." Journal of Endocrinology 130, no. 1 (July 1991): 43–51. http://dx.doi.org/10.1677/joe.0.1300043.

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ABSTRACT Differential binding of homologous and heterologous prolactin was investigated in porcine mammary tissue. Specific binding of ovine prolactin to porcine mammary membranes or tissue slices was significantly greater than specific binding of the homologous porcine prolactin. Ovine prolactin was also more potent than porcine prolactin in stimulating proliferation of Nb2 cells. In contrast, stimulation of glucose metabolism in porcine mammary explants by porcine prolactin was greater than that by ovine prolactin. Differences in specific binding were probably not due to damage during iodination, as low concentrations of iodinated prolactins were similar to unlabelled prolactins in their abilities to stimulate proliferation of Nb2 cells. Furthermore, electrophoretic analysis of medium from binding reactions suggested that differences in specific binding were not due to proteolytic cleavage of the homologous prolactin into large (> 10 kDa) fragments. These studies suggest that ovine prolactin either binds to sites in addition to the authentic lactogenic receptor in porcine mammary tissue or that a significantly higher affinity of ovine prolactin for the porcine lactogenic receptor has little effect on its biological activity. Journal of Endocrinology (1991) 130, 43–51

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34

Cui, Tingting, Sebastiaan Theuns, Jiexiong Xie, Wim Van den Broeck, and Hans J. Nauwynck. "Role of Porcine Aminopeptidase N and Sialic Acids in Porcine Coronavirus Infections in Primary Porcine Enterocytes." Viruses 12, no. 4 (April 5, 2020): 402. http://dx.doi.org/10.3390/v12040402.

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Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) have been reported to use aminopeptidase N (APN) as a cellular receptor. Recently, the role of APN as a receptor for PEDV has been questioned. In our study, the role of APN in PEDV and TGEV infections was studied in primary porcine enterocytes. After seven days of cultivation, 89% of enterocytes presented microvilli and showed a two- to five-fold higher susceptibility to PEDV and TGEV. A significant increase of PEDV and TGEV infection was correlated with a higher expression of APN, which was indicative that APN plays an important role in porcine coronavirus infections. However, PEDV and TGEV infected both APN positive and negative enterocytes. PEDV and TGEV Miller showed a higher infectivity in APN positive cells than in APN negative cells. In contrast, TGEV Purdue replicated better in APN negative cells. These results show that an additional receptor exists, different from APN for porcine coronaviruses. Subsequently, treatment of enterocytes with neuraminidase (NA) had no effect on infection efficiency of TGEV, implying that terminal cellular sialic acids (SAs) are no receptor determinants for TGEV. Treatment of TGEV with NA significantly enhanced the infection which shows that TGEV is masked by SAs.

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35

Mengeling, W. L., K. M. Lager, and A. C. Vorwald. "The effect of porcine parvovirus and porcine reproductive and respiratory syndrome virus on porcine reproductive performance." Animal Reproduction Science 60-61 (July 2000): 199–210. http://dx.doi.org/10.1016/s0378-4320(00)00135-4.

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36

Jiang, Y., and J. St John. "35 DEVELOPMENT OF INTERSPECIES CLONED MOUSE EMBRYOS RECONSTRUCTED WITH PORCINE ENUCLEATED OOCYTES." Reproduction, Fertility and Development 21, no. 1 (2009): 117. http://dx.doi.org/10.1071/rdv21n1ab35.

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Interspecies somatic cell nuclear transfer (iSCNT) offers significant opportunities for the derivation of embryonic stem cells (ESCs) that can model diseases. In this study, we investigated the development of murine-porcine iSCNT embryos and compared these to outcomes associated with porcine-porcine SCNT embryos. Enucleation was performed on in vitro-matured porcine oocytes using the Oosight spindle viewer system (CRI, UK). Either murine embryonic fibroblasts or porcine cumulus cells were injected into porcine ooplasm using a piezo-electric micromanipulator. After being exposed to the porcine ooplasm for 1 h, the reconstructed oocytes were activated by a combination of A23187 and 6-DMAP for 3.5 h. For the murine-porcine embryos (n = 9375), we used a serial culture method, namely porcine zygote medium (PZM) for 48 h followed by incubation with KSOM for another 5 days. We found that the majority of murine-porcine embryos (73.58%) arrested at the 4- to 8-cell stage, whilst a very small minority progressed through to blastocyst (0.064%). Porcine-porcine SCNT reconstructed oocytes (n = 142) cultured in PZM achieved a 15% blastocyst development rate. In the murine-porcine embryos, 10/44 embryos had chromosomal DNA loss associated with apoptosis, as determined by immunocytochemistry using the H2A.X antibody. We also found that at later stages, morula to blastocyst, the mouse-to-pig reconstructed embryos did not exhibit murine-specific Oct-4 expression patterns. Furthermore, murine mitochondrial DNA tended to be eliminated rather than preserved by the murine nuclei, which could render any subsequently derived ESCs dysfunctional due to 2 diverse genomes contributing genes to the electron transfer chain.

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Yonezawa, Naoto, Saeko Kanai-Kitayama, Tetsushi Kitayama, Ayumi Hamano, and Minoru Nakano. "Porcine zona pellucida glycoprotein ZP4 is responsible for the sperm-binding activity of the ZP3/ZP4 complex." Zygote 20, no. 4 (October 6, 2011): 389–97. http://dx.doi.org/10.1017/s0967199411000608.

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SummaryThe zona pellucida (ZP) is a transparent envelope that surrounds the mammalian oocyte and mediates species-selective sperm–egg interactions. Porcine and bovine ZPs consist of glycoproteins ZP2, ZP3, and ZP4. In both pig and bovine a heterocomplex consisting of ZP3 and ZP4 binds to sperm, however it is not clarified whether ZP3 or ZP4 in the complex is responsible for the sperm binding. Previously, we have established a baculovirus-Sf9 cell expression system for porcine ZP glycoproteins. A mixture of recombinant ZP3 (rZP3) and rZP4 displayed sperm-binding activity toward bovine sperm but not porcine sperm, probably due to differences in carbohydrate structure between the native and recombinant ZP glycoproteins. In this study, a mixture of porcine rZP3 and native ZP4 (nZP4) inhibited the binding of porcine sperm to the ZP. In contrast, a mixture of porcine nZP3 and rZP4 did not inhibit the binding of porcine sperm, although the mixture inhibited the binding of bovine sperm. The porcine rZP3/nZP4 mixture bound to the acrosomal region of porcine sperm, in a manner similar to that of the nZP3/nZP4 mixture. nZP3 was precipitated with rZP4, and nZP4 was precipitated with rZP3 by utilising the N-terminal tags on the recombinant proteins. These results indicated that nZP4, but not rZP4, is necessary for binding activity of porcine ZP3/ZP4 complex towards porcine sperm and further suggested that the carbohydrate structures of ZP4 in the porcine ZP3/ZP4 complex are responsible for porcine sperm-binding activity of the complex.

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38

FOSTIER, Bernard. "L'ÉLEVAGE PORCIN FRANÇAIS: SES PERFORMANCES TECHNIQUES EN QUESTION : la filière porcine." Bulletin de l'Académie vétérinaire de France, no. 1 (2011): 39. http://dx.doi.org/10.4267/2042/48067.

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39

Mattiuzzo, Giada, Sabrina Ivol, and Yasuhiro Takeuchi. "Regulation of Porcine Endogenous Retrovirus Release by Porcine and Human Tetherins." Journal of Virology 84, no. 5 (December 16, 2009): 2618–22. http://dx.doi.org/10.1128/jvi.01928-09.

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ABSTRACT The risk of transmission of porcine endogenous retrovirus (PERV) is one of the major safety issues in xenotransplantation. Human tetherin, recently described as an antiviral protein able to inhibit the release of enveloped viruses, and its porcine homologue were shown to inhibit PERV release from producer cells, establishing themselves as candidate molecules to suppress PERV production in porcine xenografts by animal engineering.

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40

Golovko, A. M., S. V. Derevyanko, T. O. Bova, V. I. Soroka, and V. V. Katsymon. "CONSTRUCTION OF SPECIES-SPECIFIC PRIMERS FOR MOLECULAR-GENETIC IDENTIFICATION OF PORCINE TESCHOVIRUSES AND ETEROVIRUSES A AND B." Agriciltural microbiology 10 (February 10, 2010): 156–65. http://dx.doi.org/10.35868/1997-3004.10.156-165.

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The species-specific primers for identification of taxonomic positions of strains of Porcine Teschovirus, Porcine Enterovirus A and Porcine Enterovirus B kinds were developed. The reclassification of 10 strains of Porcine Enterovirus isolated in Ukraine was performed. In conformity to the international classification they were referred to the Тeschovirus genus of Porcine Teschovirus specie. Performed investigations had created a basis for elaboration of domestic moleculargenetic diagnostic test systems

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41

Malik, Mateusz, Koen Chiers, Filip Boyen, Siska Croubels, and Dominiek Maes. "Porcine ear necrosis." Veterinary Journal 271 (May 2021): 105655. http://dx.doi.org/10.1016/j.tvjl.2021.105655.

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42

Orlyankin, B. G., and T. I. Aliper. "Porcine pathogenic viruses." "Veterinary Medicine" Journal 23, no. 01 (January 2020): 03–08. http://dx.doi.org/10.30896/0042-4846.2020.23.1.03-08.

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43

&NA;. "Porcine pancreatic extract." Reactions Weekly &NA;, no. 1205 (June 2008): 23. http://dx.doi.org/10.2165/00128415-200812050-00066.

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44

Leiman, Basil C., and Thomas E. Nelson. "PORCINE MALIGNANT HYPERTHERMIA." Anesthesiology 69, no. 3A (September 1, 1988): A412. http://dx.doi.org/10.1097/00000542-198809010-00411.

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Nelson, Thomas E. "Porcine Malignant Hyperthermia." Anesthesiology 73, no. 3 (September 1, 1990): 449–54. http://dx.doi.org/10.1097/00000542-199009000-00013.

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46

White, Mark. "Porcine self-assessment." Livestock 17, no. 3 (May 2012): 41–42. http://dx.doi.org/10.1111/j.2044-3870.2012.00115.x.

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White, Mark. "Porcine self-assessment." Livestock 17, no. 5 (September 2012): 41–42. http://dx.doi.org/10.1111/j.2044-3870.2012.00141.x.

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White, Mark. "Porcine self-assessment." Livestock 17, no. 7 (November 2012): 49–50. http://dx.doi.org/10.1111/j.2044-3870.2012.00164.x.

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White, Mark. "Porcine self-assessment." Livestock 18, no. 1 (January 2013): 41–42. http://dx.doi.org/10.1111/j.2044-3870.2012.00176.x.

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Kazarian, Kirk K., Philip W. Perdue, William Lynch, Adam Dziki, Joseph Nevola, Che-Hung Lee, Isaac Hayward, Taffy Williams, and William R. Law. "PORCINE PERITONEAL SEPSIS." Shock 1, no. 3 (March 1994): 201–12. http://dx.doi.org/10.1097/00024382-199403000-00008.

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