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Academic literature on the topic 'Polystyrene microbeads'

Author: Grafiati
Published: 6 September 2023

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Journal articles on the topic "Polystyrene microbeads"

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Valtchev, Valentin. "Core−Shell Polystyrene/Zeolite A Microbeads." Chemistry of Materials 14, no. 3 (March 2002): 956–58. http://dx.doi.org/10.1021/cm010927d.

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Tuncel, Ali, Ridvan Kahraman, and Erhan Pişkin. "Monosize polystyrene microbeads by dispersion polymerization." Journal of Applied Polymer Science 50, no. 2 (October 10, 1993): 303–19. http://dx.doi.org/10.1002/app.1993.070500212.

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Ozanich, Richard M., Kate C. Antolick, Cindy J. Bruckner-Lea, Brian P. Dockendorff, Ashton N. Easterday, Heather C. Edberg, Jay W. Grate, et al. "Use of a Novel Fluidics Microbead Trap/Flow-Cell Enhances Speed and Sensitivity of Bead-Based Bioassays." JALA: Journal of the Association for Laboratory Automation 12, no. 5 (October 2007): 303–10. http://dx.doi.org/10.1016/j.jala.2007.05.002.

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Automated devices and methods for biological sample preparation often use surface functionalized microbeads (superparamagnetic or nonmagnetic) to allow capture, purification, and preconcentration of trace amounts of proteins, cells, or nucleic acids (DNA/RNA) from complex samples. We have developed unique methods and hardware for trapping either magnetic or nonmagnetic functionalized beads that allow samples and reagents to be efficiently perfused over a microcolumn of beads. This approach yields enhanced mass transport and up to fivefold improvements in assay sensitivity or speed, dramatically improving assay capability relative to assays conducted in more traditional “batch modes” (i.e., in tubes or microplate wells). Summary results are given that highlight the analytical performance improvements obtained for automated microbead processing systems using novel microbead trap/flow-cells for various applications including (1) simultaneous capture of multiple cytokines using an antibody-coupled polystyrene bead assay with subsequent flow cytometry detection; (2) capture of nucleic acids using oligonucleotide-coupled polystyrene beads with flow cytometry detection; and (3) capture of Escherichia coli 0157:H7 from 50-mL sample volumes using antibody-coupled superparamagnetic microbeads with subsequent culturing to assess capture efficiency.
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Jinhua, Lei, and Zhou Guangyuan. "Polystyrene Microbeads by Dispersion Polymerization: Effect of Solvent on Particle Morphology." International Journal of Polymer Science 2014 (2014): 1–4. http://dx.doi.org/10.1155/2014/703205.

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Polystyrene microspheres (PS) were synthesized by dispersion polymerization in ethanol/2-Methoxyethanol (EtOH/EGME) blend solvent using styrene (St) as monomer, azobisisobutyronitrile (AIBN) as initiator, and PVP (polyvinylpyrrolidone) K-30 as stabilizer. The typical recipe of dispersion polymerization is as follows: St/Solvent/AIBN/PVP = 10 g/88 g/0.1 g/2 g. The morphology of polystyrene microspheres was characterized by the scanning electron microscopy (SEM) and the molecular weights of PS particles were measured by the Ubbelohde viscometer method. The effect of ethanol content in the blend solvent on the morphology and molecular weight of polystyrene was studied. We found that the size of polystyrene microspheres increased and the molecular weight of polystyrene microspheres decreased with the decreasing of the ethanol content in the blend solvent from 100 wt% to 0 wt%. What is more, the size monodispersity of polystyrene microspheres was quite good when the pure ethanol or pure 2-Methoxyethanol was used; however when the blend ethanol/2-Methoxyethanol solvent was used, the polystyrene microspheres became polydisperse. We further found that the monodispersity of polystyrene microspheres can be significantly improved by adding a small amount of water into the blend solvent; the particles became monodisperse when the content of water in the blend solvent was up to 2 wt%.
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Li, Lichao, Huaihe Song, and Xiaohong Chen. "Hollow carbon microspheres prepared from polystyrene microbeads." Carbon 44, no. 3 (March 2006): 596–99. http://dx.doi.org/10.1016/j.carbon.2005.09.035.

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Foti, Leonardo, Andre Sionek, Elis Moura Stori, Paula Poli Soares, Miriam Marzall Pereira, Marco Aurélio Krieger, Cesar Liberato Petzhold, et al. "Electrospray induced surface activation of polystyrene microbeads for diagnostic applications." Journal of Materials Chemistry B 3, no. 13 (2015): 2725–31. http://dx.doi.org/10.1039/c4tb01884b.

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Proposed electrochemical reaction mechanism: (a) highly charged microbeads approach the electrolyte; (b) microbeads sink and are solvated by water molecules; (c) water oxidation reaction disrupts PS surface bonds; (d) oxygen is incorporated into the polymer chains.
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Ren, Zhi Min, Xi Nie, and Sheng Shu Ai. "Influence of Blocking Agents on Non-Specific Background of Polystyrene Microbeads in Serum Immunoassay." Advanced Materials Research 641-642 (January 2013): 858–61. http://dx.doi.org/10.4028/www.scientific.net/amr.641-642.858.

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In this paper, we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents. The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA, 0.8% polyvinylpyrrolidone, 0.05% Tween-20, PBS phosphate buffer, pH7.0) for two hours at room temperature.
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Nie, Libo, Fuhua Liu, and Hongcheng Yang. "Preparation of Quantum Dot Fluorescence Encoded Polystyrene Microbeads." Nanoscience and Nanotechnology Letters 9, no. 6 (June 1, 2017): 941–44. http://dx.doi.org/10.1166/nnl.2017.2416.

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Gambardella, Chiara, Silvia Morgana, Sara Ferrando, Mattia Bramini, Veronica Piazza, Elisa Costa, Francesca Garaventa, and Marco Faimali. "Effects of polystyrene microbeads in marine planktonic crustaceans." Ecotoxicology and Environmental Safety 145 (November 2017): 250–57. http://dx.doi.org/10.1016/j.ecoenv.2017.07.036.

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Park, S. J., S. W. Jun, A. R. Kim, and Y. H. Ahn. "Terahertz metamaterial sensing on polystyrene microbeads: shape dependence." Optical Materials Express 5, no. 10 (September 8, 2015): 2150. http://dx.doi.org/10.1364/ome.5.002150.

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Dissertations / Theses on the topic "Polystyrene microbeads"

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Liong, Rolan Yuk Loong. "BACTERIAL GROWTH ON METAL AND NON-METAL SURFACES IN A STATIC BIOREACTOR." DigitalCommons@CalPoly, 2013. https://digitalcommons.calpoly.edu/theses/923.

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Research was conducted to observe bacterial growth on the surface of metals in a static bioreactor. Metal and non-metal samples were subjected to bacterial exposure (1 day and 9 days). The metal samples were surface treated prior to bacterial exposure. The microstructures of the surface treated samples were analyzed by optical microscopy. After exposure, the microstructures of the samples were analyzed by scanning electron microscopy (SEM). The analysis suggested that microbial attachment on the surface was related to the underlying microstructure of steel. The preferential attachment of microbes could potentially be influenced by cathodic and anodic regions created by the electrolytic cells.
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Book chapters on the topic "Polystyrene microbeads"

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Nagy, Péter, Géza I. Márk, and Erzsébet Balázs. "Determination of SPM TIP Shape Using Polystyrene Latex Balls." In Microbeam and Nanobeam Analysis, 425–33. Vienna: Springer Vienna, 1996. http://dx.doi.org/10.1007/978-3-7091-6555-3_35.

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Conference papers on the topic "Polystyrene microbeads"

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Chang, Jin, Bingbo Zhang, Dena Li, Guiping Ma, Weicai Wang, and Qi Zhang. "Preparation and Characterization of Tricolor CdSe-Tagged Microbeads for Bio-Detection." In 2007 First International Conference on Integration and Commercialization of Micro and Nanosystems. ASMEDC, 2007. http://dx.doi.org/10.1115/mnc2007-21138.

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Tricolor microbeads for biological assay have been prepared by embedding three quantum dots (cadmium selenide semiconductor nanocrystals) of different size into carboxyl-functionalized polystyrene (PS-COOH) microbeads. These efforts can render CdSe nanocrystals water-solubility, chemical stability and good photostability. The results indicate that QDs-tagged microbeads are highly uniform, reproducible and strong in fluorescence emission. Based on the properties it possesses, QDs-tagged microbead may have great potential for bio-detection.
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Wang, Hai-Qiao, Zhen-Li Huang, Tian-Cai Liu, Jian-Hao Wang, Yuan-Di Zhao, and Qing-Ming Luo. "Quantitative doping of commercial polystyrene microbeads with quantum dots." In ICO20:Biomedical Optics, edited by Gert von Bally and Qingming Luo. SPIE, 2006. http://dx.doi.org/10.1117/12.667679.

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Mulero, Rafael, Alejandro Moraga, and Min Jun Kim. "High Resolution Detection and Configuration of Bacteria Using Microscale Pores." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-41199.

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A novel method for detecting and configuring bacteria using a micro-scale pore is presented. The method distinguishes between different species of bacteria by measuring the ionic current blockage (resistive pulse) as bacteria electrophoretically translocate the micro-pore. Both wild-type flagellated (HCB 33) and non-flagellated Escherichia coli (HCB 5), and Polystyrene microbeads were used in this study to demonstrate the efficacy of this method. High resolution electrical signal readout enabled discrimination of the orientation of both non-flagellated and peritrichously flagellated bacteria as they move through the solid-state pore.
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Kumar, G. Naga Siva, Sushanta K. Mitra, and V. Ramgopal Rao. "Fabrication of Dielectrophoretic Microfluidic Device." In ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2009. http://dx.doi.org/10.1115/icnmm2009-82170.

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Technological needs of the recent times require the improvement in micro-scale devices that manipulate the bioparticles like cells, bacteria, viruses, DNA, proteins, etc. Such devices have diverse and widespread applications in biomedical, drug delivery and diagnostics for separating, trapping, sorting and mixing of particles. Dielectrophoresis (DEP) is one of the techniques used for manipulating the particles in a nonuniform electric field. In the present study, fabrication and characterization of microfluidic device for DEP is analyzed and experimented. An overview of fabrication techniques which can be used for making of DEP device is provided with experimental details. DEP microfluidic device is fabricated by preparing channels and microelectrodes on PDMS and glass materials respectively. Oxygen plasma treatment has been used for bonding the PDMS channel and micro-electrode patterned glass substrate. Further experiments are conducted to demonstrate the DEP principle with polystyrene microbeads. The movement of microbeads towards the high electric field strengths at 12Vpp and 10 MHz frequency is observed. Characterizing equipments like ellipsometer, profilometer, scanning electron microscopy, contact angle measurement systems were used for measuring oxide layer thickness, width and depth of the channels, surface characteristics etc., during fabrication.
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Capurro, Jorge J., and Hongseok Moses Noh. "Characterization of AC Electrokinetic Forces Using Pressure-Driven Flow in a Microchannel." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-68984.

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Ac electrokinetics is a versatile technique for particle and fluid manipulation in microfluidic environment. However, analyzing and predicting particle motions due to the ac electrokinetic effects is a difficult task because it requires the quantitative understanding of multiple phenomena such as dielectrophoresis (DEP), ac electroosmosis (ACEO), and electrothermal effects (ETE). In this paper we present a force balance approach to analyze ac electrokinetic effects, particularly ACEO. Pressure-driven flows were used to quantify the ACEO and DEP forces acting on a particle. Polystyrene microbeads suspended in KCl solution were introduced in polydimethylsiloxane (PDMS) microchannels attached to a glass plate with gold microelectrodes. The microbeads were initially collected and aligned along the center of the electrodes at 1 kHz and 1 Vp-p, and then a well-controlled pressure-driven flow was introduced resulting in the translation of the particles. Particles moved to a new location where a new force balance is reached. This particle translation on the surface of the electrode was carefully monitored as varying the applied flow rate. The net force due to ac electrokinetic effects at different locations over the electrode was calculated using the experimental data and the force balance relationship.
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Bhardwaj, Priyank, Piyush Bagdi, Ashish S. Sharma, and Ashis K. Sen. "Microfluidic Chip for Particle-Liquid Separation." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-62343.

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This paper presents theoretical analysis, design, fabrication and test of a microfluidic device (‘Micro hydrocyclone’) for separation of micron and sub-micron size solid particles from liquid in a particle-liquid mixture. A theoretical analysis of the micro hydrocyclone is performed to understand the physics and develop suitable design models. The structure of the proposed device is designed based on Bradley model, as it offers lower cut-size thus making it suitable for microfluidics applications. The operational parameters are derived from the dimensional group model. The device is fabricated with SU-8 photoresist on PMMA substrate using a combination of photolithography and micro-milling. Experiments are performed to demonstrate particle-liquid separation using polystyrene microbeads suspended in PBS as the feed sample. The influence of inlet velocity and particle size on particle separation efficiency is investigated. The proposed device can be easily integrated with micro-environments thus is suitable for lab-on-chip and microsystems development. The device may have applications in chemical analysis, materials research, point-of-care, blood sample preparation and other biomedical applications.
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Lee, Doh-Hyoung, Jonghyun Oh, Robert Hart, Bakhtier Farouk, and Hongseok Moses Noh. "A Study of AC Electrokinetic Phenomena Under DC Electroosmotic Flows." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-68969.

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AC electrokinetic phenomena have drawn much attention in the microfluidics and lab-on-a-chip communities since these techniques have a great potential for effective manipulation of small particles (micro- to nanoscale particles, polymer beads to biological cells and molecules) and fluids in microchannel environments. One unanswered question is how the AC electrokinetic phenomena are affected by DC electroosmotic flows that are often employed in lab-on-a-chip systems as a pumping method. This paper presents experimental and numerical studies on the interaction between AC electrokinetic phenomena and DC electroosmotic flows. The motions of polystyrene microbeads suspended in deionized water in a microchannel were studied as the main AC and DC electrokinetics parameters were varied. Numerical simulations of flow field were performed using COMSOL Multiphysics software. The forces considered in the numerical simulation include electrophoresis, DC electroosmosis, dielectrophoresis, AC electroosmosis, electrothermal effect, diffusion, Stokes drag force, and gravity. The numerical simulation results showed good agreements with experimental data. We believe that this study will contribute to the understanding of the interactions between DC and AC electrokinetic phenomena and thus enable researchers to develop powerful microdevices based on the combination of these two techniques.
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Cheang, U. Kei, Jun Hee Lee, Paul Kim, and Min Jun Kim. "Magnetic Control of Biologically Inspired Robotic Microswimmers." In ASME-JSME-KSME 2011 Joint Fluids Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajk2011-19014.

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Bacterial flagella have been employed as nanoactuators for biomimetic microswimmers in low Reynolds number fluidic environments. The microswimmer is a dumbbell-like swimmer that utilizes flagellar hydrodynamics to achieve spiral-type swimming. Flagellar filaments from Salmonella typhimurium are harnessed and functionalized in order to serve as couplers for polystyrene (PS) microbeads and magnetic nanoparticles (MNPs) using avidin-biotin chemistry. The MNP have an iron oxide core that will allow us to actuate the microswimmer under a rotating magnetic field. Using a micromanufacturing process, microswimmer of different configurations can be created to mimic mono- and multi-flagellated bacteria. A magnetic control system consists of electromagnetic coils arranged in an approximate Helmholtz configuration was designed, constructed, and characterized. In conjunction with a LabVIEW input interface, a DAQ controller was used as a function generator to generate sinusoidal waveforms to the power supplies. AC current outputs were supplied from the power supplies to the coils in order to generate a rotating magnetic field. A rotating magnetic field will induce rotation in the flagella conjugated MNP which in term will rotate the flagellar filament into a spiral configuration and achieve propulsion, as in polarly-flagellated bacteria. A high-speed camera provided real-time imaging of the microswimmer motion in a static fluidic environment inside a closed PDMS (Polydimethylsiloxane) chamber. The microswimmers exhibited flagellar propulsion in a low Reynolds number fluidic environment under a rotating magnetic field, which demonstrates its potential for biomedical applications.
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Karavaeva, O. A., O. I. Guliy, V. V. Simakov, A. K. M. Alsowaidi, A. A. Khomyakova, O. Yu Ksenofontova, and A. V. Smirnov. "Prospects the use of polystyrene films for the immobilization of bacteria." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.114.

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Haghshenas-Jaryani, Mahdi, Nguyen T. Tran, Alan P. Bowling, James A. Drake, and Samarendra Mohanty. "Multiscale Modeling and Simulation of a Microbead in an Optical Trapping Process." In ASME 2013 2nd Global Congress on NanoEngineering for Medicine and Biology. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/nemb2013-93059.

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The purpose of this work is to generate a theoretical model for the dynamics of a polystyrene microsphere under the influence of Gaussian beam optical tweezers (OTs) in the ray-optics regime. OTs use the radiation pressure from a focused laser beam to manipulate microscopic objects as small as atoms [1]. They have been used in the biological sciences to measure nanometer-range displacements, apply picoNewton-range forces, and determine the mechanical properties of DNA, cell membranes, whole cells, and microtubules. The proposed model takes into account the forces and moments imparted onto the microbead by the OTs beam, and uses a Newton-Euler Dynamics framework to generate the equations of motion. Although examination of dimensionless numbers and other indicators including, Reynolds number 10−9 ≤ Re ≤ 10−4, Knudsen number 0.0001875, and the disproportionality between the mass and the viscous drag co-efficients O(10−4), does not clearly indicate whether this is a multiscale problem or not; but, a numerical integration of the original model leads to a long simulation run-time, a few days. Moreover, investigation of the step size showed that the adaptive numerical integrator was proceeding with a picosecond step size in order to achieve the requested accuracy. This situation implies a multiscale feature involved in the dynamics of optical trapping process of the small bead. To address this issue, a multiscale model is developed that helps to significantly reduce the simulation run-time and reveals underdamped behavior of the bead. In order to verify the theoretical model, experiments were carried out on a microsphere bead with 1.6μm diameter. A comparison of experimental data and simulation data indicate that this approach closely models microparticle behavior to the accuracy of the experiment under Gaussian beam optical tweezers.
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Reports on the topic "Polystyrene microbeads"

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Ding, Zheng-You, Shenmin Ma, Dennis Kriz, J. J. Aklonis, and R. Salovey. Model Filled Polymers .11. Synthesis of Uniformly Crosslinked Polystyrene Microbeads. Fort Belvoir, VA: Defense Technical Information Center, June 1991. http://dx.doi.org/10.21236/ada237472.

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