Dissertations / Theses on the topic 'Polarized light microscope'
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Santa, Nestor. "Demonstration of Optical Microscopy and Image Processing to Classify Respirable Coal Mine Dust Particles." Thesis, Virginia Tech, 2021. http://hdl.handle.net/10919/103919.
Full textM.S.
Inhalation of fine particles in underground coal environments can lead to chronic lung diseases, such as coal worker’s pneumoconiosis or progressive massive fibrosis (PMF), which is the most severe form of disease. During the last two decades, the rates of reported cases of PMF in underground coal miners have more than doubled. Many authors have suggested different reasons to explain this trend, including the extraction of thinner coal deposits, mining techniques, changes in mineral content, and the use of high-powered cutting equipment. However, detailed information of specific dust constituents and monitoring the variability of dust concentrations during work shifts are needed to determine possible dust sources and comprehend the more recent changing disease patterns. A dust-monitoring system that provides accurate and timely data on specific respirable coal mine dust (RCMD) constituents would enable the deployment of effective control strategies to mitigate exposure to respirable hazards. Optical microscopy (OM) has been used for a long time to analyze and identify dust particles. More recent advances in portable microscopy have allowed the microscope analysis to be implemented in the field. On the other hand, automated image processing techniques are rapidly progressing and powerful imaging hardware has become a reality in handy small devices. OM and image processing technologies offer a path for near real-time applications that have not been explored for RCMD monitoring yet. In this work, a novel monitoring concept is explored using OM and image processing to classify RCMD particles. Images from dust samples captured with a polarizing microscope were used to build a classification model based on optical properties. The method herein described showed outstanding accuracy for separating coal and mineral fractions. Additionally, the Identification of silica particles in the mineral fraction was investigated and has proved more challenging. A particular finding suggests that particle loading density in the images plays an important role in classification accuracy.
Cao, Shuiyan. "Using plasmonic nanostructures to control electrically excited light emission." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS042/document.
Full textIn this thesis, we use different plasmonic nanostructures to control the emission of electrically-excited light. Our electrical emission is from an “STM-nanosource” which uses the inelastic tunnel current between the tip of a scanning tunneling microscope (STM) and a metallic sample, to locally excite both localized and propagating surface plasmon polaritons. The interaction of our STM-nanosource and a circular plasmonic lens (a series of concentric slits etched in a thick gold film) produces a radially polarized microsource of low angular spread (≈±4°). The influence of the structural parameters on the angular spread of the resulting microsource is also investigated. In addition, a low angular spread (<±7°) for a large wavelength range (650-850 nm) is achieved. Thus this electrically-driven microsource of nearly collimated light has a broad spectral response and is optimal over a wide energy range, especially in comparison with other resonant plasmonic structures such as Yagi-Uda nanoantennas. The interaction of our STM-nanosource and an elliptical plasmonic lens (a single elliptical slit etched in a thick gold film) is also studied. When the STM excitation is located at the focal point position of the elliptical plasmonic lens, a directional light beam of low angular spread is acquired. Moreover, in the experiment we find that by changing the eccentricity of the elliptical plasmonic lens, the emission angle is varied. It is found that the larger the eccentricity of the elliptical lens, the higher the emission angle. This study provides a better understanding of how plasmonic nanostructures shape the emission of light. The interaction of STM-excited SPPs and a planar plasmonic multi-layer stack structure is also investigated. It is demonstrated that using STM excitation we can probe the optical band structure of the Au-SiO₂-Au stack. We find that the thickness of the dielectric plays an important role in changing the coupling between the modes. We also compare the results obtained by both laser and STM excitation of the same stack structure. The results indicate that the STM technique is superior in sensitivity. These findings highlight the potential of the STM as a sensitive optical nanoscopic technique to probe the optical bands of plasmonic nanostructures. Finally, the interaction of an STM-nanosource and an individual triangular plate is also studied. We find that when the STM excitation is centered on the triangular plate, there is no directional light emission. However, when the STM-nanosource is located on the edge of the triangle, directional light emission is obtained. This study provides us a novel avenue to achieve directional light emission. We also study probing the optical LDOS of the triangle with the STM-nanosource. Thus, our results show that the manipulation of light is achieved through SPP-matter interactions. Using plasmonic nanostructures, we control the collimation, polarization, and direction of the light originating from the STM-nanosource
Gomes, Claudia Messias. "Influência da diminuição da temperatura sobre o fuso meiótico de oócitos de camundongas e de mulheres maturados in vitro." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/5/5139/tde-22072011-132252/.
Full textIntroduction: The meiotic spindle of most mammals is sensitive to cooling and depolymerizes even after a slight reduction in temperature. This is well described and studied on matured oocytes at metaphase II (MII). However, little is known about the influence of low temperatures under meiotic spindle of imature oocytes. In this way, we sougth to evaluate: 1) the influence of low temperatures on mice oocyte meiotic spindle matured in vitro e 2) the oocyte meiotic spindle from human oocytes matured in vitro and cryopreserved by slow-rate freezing or vitrification at GV stage. Methods: Two experiments were done: the first one on mice and the second one on women.At experiment 1, immature mice oocytes at metaphase I (MI), telophase I (TI) and MII were cultured at 37º C (control), room temperature (22oC) and 4º C for 0, 10, 30 and 60 minutes and then spindle analysis was made with polarized light microscopy (PLM) (LC-Polscope-Oosight image software) or immunocytochemistry (ICC). At experiment 2, GV oocytes retrieved from women submitted to ovulation induction and in vitro fertilization were randomly divided in three groups: fresh oocytes (A), cryopreserved by slow-freezing (B) and cryopreserved by vitrification (C). Fresh, thawed and warmed oocytes were matured in vitro to metaphase II oocytes (MII). A meiotic spindle analysis was done by polarized light microscopy (ICSI Guard Octax). Results: Experiment 1: At time 0 min and 37º C, all oocytes had polymerized spindles both at PLM or ICC. At 4º C, the number of MI oocytes with detectable spindles at PLM was smaller than those analysed by ICC, and it decreased with time, which had also occured with TI oocytes at a smaller proportion. However, at 4º C, TI meiotic spindle recognition with polarized light microscopy and ICC was comparable. When MII oocytes were cultured at 4º C, the spindle visualization decreased proportionally in correlation with culture time at PLM, and the same happened with ICC in a less pronounced manner. At room temperature there was a little descrease regarding visualization of meiotic spindle, both at PLM and ICC, altought it was not significant for TI oocytes. Experiment 2: Oocyte survival immediately after thawing/warming were 44.6% for group B and 79% for group C. After 24 hours of culture, oocyte survival was 29.2% and 69%, respectively. The median time for maturation was 26 hours for groups A and C, and 27 hours for group B. The percentage of MII after maturation in vitro were smaller in group B and similar between groups A and C. The same oocured for spindle visualization which were lower in group B and similar between groups A and C. Conclusions: There was a difference on the percentages of meiotic spindle depolymerization in response to cooling in mice oocytes at different stages of meiotic division. Spindle depolymerization was lower in TI. Also, meiotic spindle depolimerization was proportional to culture time, except for TI oocytes at room temperature.Vitrified GV oocytes had a better survival when warmed, compared to slow-rate frozen oocytes. Vitrified GV oocytes had similar maturation in vitro rates and polymerized spindles detection when compared to fresh oocytes
MacDonald, Donia J. "Wall characteristics of saccular aneurysms from polarized light microscopy." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0006/MQ42172.pdf.
Full textМоскаленко, Роман Андрійович, Роман Андреевич Москаленко, Roman Andriiovych Moskalenko, I. Iashchіchyn, M. Fallah, and Artem Mykhailovych Piddubnyi. "Verification of corpora amylacea amyloid nature via polarized light microscopy." Thesis, Sumy State University, 2015. http://essuir.sumdu.edu.ua/handle/123456789/41281.
Full textBaba, Justin Shekwoga. "The use of polarized light for biomedical applications." Texas A&M University, 2003. http://hdl.handle.net/1969.1/1206.
Full textBurgio, Lucia. "Analysis of pigments on art objects by Raman microscopy and other techniques." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369123.
Full textLi, Yanfang. "A study on the use of polarized light in application to noninvasive tissue diagnoistics /." See Full Text at OhioLINK ETD Center (Requires Adobe Acrobat Reader for viewing), 2005. http://www.ohiolink.edu/etd/view.cgi?toledo1134596719.
Full textTypescript. "A dissertation [submitted] as partial fulfillment of the requirements of the Doctor of Philosophy degree in Engineering." Bibliography: leaves 106-120.
Lima, Júlia Magalhães da Costa. "Análise da profundidade de desgaste e da perda mineral no esmalte subjacente à microabrasão após técnica microabrasiva." Universidade Federal da Paraíba, 2009. http://tede.biblioteca.ufpb.br:8080/handle/tede/6669.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
The main effect of the microabrasion in the enamel is significant erosion. However, there is a gap in the literature about validated and reproducible assessment of the depth of erosion in the enamel surface which is originally curve. AIMS: Evaluate depth of erosion and mineral loss of enamel produced by microabrasion technique in original coronary surface of human teeth. METHODS AND MATERIALS: 40 extracted human molars were randomly spited in four groups, with 10 specimens each, in accordance with the microabrasive treatment: AC- 18% hydrochloric acid and pumice, AF 37% phosphoric acid and pumice, OP Opalustre and WRM Whiteness RM. Each specimens had buccal surface´s laterals isolated so that the central area received the microabrasion treatment. After this procedure, transverse slices not demineralized were prepared and submitted to microradiography and analysis in Polarized Light Microscope. One own terminology had created for the morphology of the interface enamel normal-microabrasioned. This served as base to introduction of a profilometry technique with analysis of digital images, in order to get the depth of erosion on microabrasion´s area. The Intraclass Correlation Test was applied to test technique´s reproducibility. The mineral loss and the depth which it happened had analyzed by transverses plotted at equidistant points of the limit enamel normal-microabrasioned. The dates were analyzed with ANOVA test (p < 0.05). RESULTS: The profilometry technique achieved a good reproducibility (Intraclass Correlation Test of 0,9998) and was validated internally. The AC group was the most aggressive, with a greater depth of erosion (110,51 ± 41,21 μm), and a greater mineral loss (13 ± 3 peso %), with significant difference between WRM group (p < 0,05; 9,41± 4,4 peso %) and OP group (p < 0,05; 9,0 ± 3,8 peso %). The OP group, on the other hand, was the less aggressive, with the lowest values in all parameters analyzed, presenting depth of erosion less than AC group (p < 0,0001), WRM group (p < 0,001; 86,24 ± 27,99 μm) and AF group (p < 0,05; 74,46 ± 42,06 μm). The others two groups achieved intermediate results for depth of erosion and mineral loss. The depth of mineral loss was greater than on AF group (31,38 ± 20,30 μm), however, there wasn´t statistical difference between the groups. CONCLUSIONS: Based on own terminology for the interface enamel normalmicroabrasioned and on the implementation of new technique of profilometry, the agents tested showed a significant difference in the depth of erosion, which was consistent with the mineral loss. However, there wasn´t difference in the depth of mineral loss. Furthermore the new technique of profilometry is proposed to fill a gap in the literature, allowing the determination of physical depth of erosion in areas naturally curves of hard biological tissues.
O principal efeito da microabrasão no esmalte dental é uma erosão significativa. Porém, existe uma lacuna na literatura no que concerne à avaliação validada e reprodutível da profundidade de desgaste na superfície dental natural. OBJETIVOS: Avaliar a profundidade de desgaste e a perda mineral do esmalte dentário resultante da técnica de microabrasão na superfície coronária original de dentes humanos. MATERIAIS E MÉTODOS: 40 terceiros molares humanos extraídos foram divididos aleatoriamente em 4 grupos, de 10 espécimes cada, de acordo com o material microabrasivo utilizado: AC - ácido clorídrico a 18% e pedra-pomes, AF - ácido fosfórico a 37% e pedra-pomes, OP - Opalustre® e WRM - Whiteness RM®. Cada elemento teve as laterais da face vestibular protegidas para que apenas a área central fosse exposta aos agentes microabrasivos. Após o procedimento de microabrasão, cortes transversais não desmineralizados foram preparados e submetidos à radiomicrografia e análise em Microscopia de Luz Polarizada. Uma terminologia própria foi formulada para a morfologia da interface esmalte normalmicroabrasionado. Esta serviu de base à introdução de uma Técnica de Perfilometria com Análise de Imagens Digitais, com o intuito de obter a profundidade de desgaste ao longo da área microabrasionada. O teste de correlação intraclasse foi aplicado para testar a reprodutibilidade da técnica. A quantidade da perda mineral e a profundidade em que esta ocorreu foram analisadas em transversais traçadas em pontos eqüidistantes do limite esmalte normal-microabrasionado. Os dados obtidos foram analisados com o teste ANOVA (p < 0,05). RESULTADOS: A Técnica de Perfilometria obteve uma boa reprodutibilidade (coeficiente de correlação intraclasse de 0,9998) e foi validada internamente. O grupo AC foi o mais agressivo, apresentando a maior profundidade de desgaste (110,51 ± 41,21 μm), e a maior perda mineral (13 ± 3 peso %), com diferenças significantes em relação aos grupos WRM (p < 0,05; 9,41± 4,4 peso %) e OP (p < 0,05; 9,0 ± 3,8 peso %). O grupo OP, por outro lado, foi o menos agressivo com os menores valores para todos os parâmetros analisados, apresentando uma profundidade de desgaste menor em relação aos grupos AC (p < 0,0001), WRM (p < 0,001; 86,24 ± 27,99 μm) e AF (p < 0,05; 74,46 ± 42,06 μm). Os outros dois grupos apresentaram resultados intermediários para profundidade de desgaste e quantidade de perda mineral. Não houve diferença quanto à profundidade de perda mineral CONCLUSÃO: Com base em uma terminologia própria para a interface esmalte normal-microabrasionado e na aplicação de uma nova Técnica de Perfilometria, os agentes testados mostraram uma significativa diferença quanto à profundidade de desgaste, que foi condizente com a perda mineral. A nova Técnica de Perfilometria propõe o preenchimento de uma lacuna na literatura, permitindo a determinação física de profundidade de desgaste em superfícies naturalmente curvas de tecidos biológicos duros.
Lucisano, Marília Pacifico. "Efeito do uso sistêmico de alendronato sódico no tecido ósseo e nas estruturas dentárias mineralizadas: estudo químico, mecânico e morfológico, em modelo murino." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/58/58135/tde-04022011-114321/.
Full textBisphosphonates represent a class of drugs that act on bone metabolism and are widely used in the prevention and treatment of osteopenic and osteoporotic states. The objectives of this study were to evaluate, in vivo, the effect of the systemic use of sodium alendronate on: the mineral bone density of rats, by radiographic optical densitometry and dual-energy x-ray absorptiometry (DXA); the mineralized dental structures of murine incisors, by analysis of infrared (IR) spectrometry, fluorescence spectroscopy, cross-sectional microhardness (CSMH), scanning electron microscopy (SEM) and polarized light microscopy (PLM). Forty-five Wistar rats aged 36-42 days and weighing 200-230 g were assigned to two groups: experimental (n= 25) and control (n= 20). The experimental group received two weekly doses of 1 mg/kg of chemically pure sodium alendronate diluted in distilled water, via gavage, while the animals of the control group received only distilled water. After 60 days, the animals were killed by anesthetic overdose, and the maxillary incisors were extracted and the tibias were removed. The mineral bone density of the tibias was analyzed radiographically and by DXA. The maxillary incisors were subjected to the following evaluations: chemical analysis by IR spectrometry and fluorescence spectroscopy, enamel and dentin CSMH, SEM and PLM. The results were subjected to statistical analysis by the Kruskal-Wallis non-parametric test, using the SAS (Statistical Analysis System) software for Windows version 9.1.3. The significance level was set at 5%. The experimental group presented higher mineral bone density (p<0.05) than the control group, by radiographic optical densitometry and DXA. The chemical analysis by IR spectrometry and fluorescence spectroscopy revealed the presence of alendronate in the mineralized dental structure of the specimens of the experimental group, with a percentage of incorporation of 0.0018% per tooth. The results of enamel and dentin CSMH did not show statistically significant difference between the experimental and control groups (p>0.05). There were no significant morphological differences among the specimens of the groups by SEM and PLM. Based on the obtained results, it may be concluded that the treatment with sodium alendronate caused an increase in the mineral bone density of the proximal tibial metaphysis, and that alendronate was incorporated in the mineralized dental structures without causing significant effects in the enamel and dentin microhardness and morphology of rat incisors.
Scott, Michael L. "Determining the Air Void Parameters of Concrete Using Digital Image Analysis of Polarized Light Micrographs." Thesis, Virginia Tech, 1997. http://hdl.handle.net/10919/30547.
Full textMaster of Science
Henderson, John J. "Microhistology of moose browse using polarized light microscopy and age & sex-determination using moose scat." University of Nebraska at Kearney, 2013.
Find full textMoody, Hannah Leigh. "Detection of condom lubricants and starches in the presence of biologicals by diffuse reflectance infrared fourier transform spectroscopy and polarized light microscopy." Thesis, Boston University, 2013. https://hdl.handle.net/2144/21221.
Full textCondoms have been used in sexual assaults as a means of preventing the transmission of biological fluids. Current sexual assault evidence collection kit processing protocols do not regularly take advantage of the information that can be gathered by examining residues left by condoms during intercourse. A biphasic liquid-liquid extraction technique was developed to separate polar and non-polar condom residues, which had been collected on cotton tipped swabs. This research involved the examination of twenty condom brands by Diffuse Reflectance Infrared Fourier Transform Spectroscopy. Five brands were selected to examine the consistency of this technique when the lubricants were exposed to body and storage temperature conditions for various times and in the presence of oral, vaginal, and blood samples. Additionally, starches collected from the condoms under each of the above conditions were examined. Although all lubricants were identifiable using this IR technique, the nonoxynol-9 (spermicide) containing samples produced spectra which were not identical to those produced by nonoxynol-9 standards. Although there was a decrease in the percent transmittance within IR spectra as the time between the collection and the extraction of the swabs increased, the condom residues of interest remained identifiable at all time points examined. The use of vaginal and oral swabs in the collection caused a negligible amount of background interference, which could be eliminated through spectral subtraction of the swab.
2031-01-01
Hao, Alvin Chua Sern. "Miróbriga: study of roman mortars." Master's thesis, Universidade de Évora, 2018. http://hdl.handle.net/10174/24466.
Full textOliveira, Roberto Sotto-Maior Fortes de. "Arranjo das fibras gengivais transeptais de ratos Wistar: estudo histomorfométrico e histoquímico." Universidade Federal de Juiz de Fora (UFJF), 2010. https://repositorio.ufjf.br/jspui/handle/ufjf/5549.
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FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
As fibras transeptais situam-se na lâmina própria da gengiva entre dois dentes adjacentes. São comumente descritas como um grupo de fibras colágenas inseridas no cemento de um dente que seguem diretamente por sobre a crista óssea alveolar inserindo-se em uma região correspondente no cemento do dente adjacente. De maneira distinta, relatou-se na literatura que as fibras transeptais não são contínuas ao longo de toda sua extensão; são originadas dos dentes adjacentes, entrelaçando-se na região central do espaço interproximal. Com o presente estudo, objetivou-se avaliar e quantificar o arranjo das fibras gengivais transeptais empregando-se análise histomorfométrica e histoquímica em cortes histológicos sagitais da região entre primeiro e segundo molares da maxila de 12 ratos Wistar machos. As fibras colágenas foram coradas pelo método de picro-sirius e analisadas por microscopia de luz polarizada linear, processamento e análise digital de imagem baseados em métodos de filtragem e medição por transformada de Fourier. Foram realizadas medidas histomorfométricas de orientação, densidade e dimensão fractal das fibras. O brilho máximo apresentado pelas fibras foi mensurado pelo histograma de intensidade. Os parâmetros quantitativos foram comparados entre três regiões de interesse ao longo da extensão da gengiva interproximal ocupada pelas fibras transeptais utilizando-se análise de variância (ANOVA) seguida de testes post hoc de Bonferroni ou Tamhane T2. Houve um aumento significativo (p < 0,05) nos parâmetros de densidade de ocupação e dimensão fractal das fibras pertencentes à região central quando comparada às regiões próximas aos dentes, sem haver diferença na orientação e na intensidade de brilho das fibras. Os resultados demonstraram quantitativamente um arranjo mais denso de fibras na região central, reforçando o conceito de que as fibras transeptais entrelaçam-se nesta região.
The transeptal fibers are located in the lamina propria of the gingiva between adjacent teeth. They are often described as a group of collagen fibers embedded in the cementum of one tooth that follows a straight path over the alveolar bone crest and embeds itself in the cementum of the adjacent tooth. In a distinct way, it was reported in the literature that the transeptal fibers are not continuous over its full length but originates from the adjacent teeth interlacing in the central area of the interproximal gingiva. The present study aimed to evaluate and quantitate the gingival transseptal fibers arrangement by histomorphometry and histochemistry of sagittally cut tissue sections of the interdental region between first and second maxilar molar teeth of 12 male Wistar rat. The collagen fibers were stained with Picrosirius red staining and assessed with linear polarized light microscopy, digital image processing and analysis based on Fourier transform measurements and filtering techniques. Histomorphometrical parameters like fiber orientation, area fraction and fractal dimension were measured. The maximal brilliance presented by the fibers was evaluated using intensity histogram measurements. These quantitative parameters were compared among three different regions of interest along the length of the interdental gingiva using one-way analysis of variance (ANOVA) and Bonferroni’s or Tamhane’s T2 post hoc tests. There was an increase (p < 0.05) in area fraction and fractal dimension parameters in the central region compared to regions near the teeth, and no difference in the orientation and maximal brilliance of fibers among the tree regions. Our results quantitative demonstrated a denser arrangement of the fibers in the central region reinforcing the model of an interlacement of the transseptal fibers in that region.
Kilani, Suha School of Medicine UNSW. "The use of polarised light microscopy as a non-invasive tool for early assessment of human oocytes and embryos." Awarded by:University of New South Wales. School of Medicine, 2007. http://handle.unsw.edu.au/1959.4/35247.
Full textTruong, Ba Luu. "Organisation tri-dimensionnelle des cellules myocardiques au cours du développement ventriculaire fœtal et postnatal." Thesis, Université Grenoble Alpes (ComUE), 2017. http://www.theses.fr/2017GREAS011/document.
Full textThe 3D architecture of the ventricular mass is poorly known, although in vivo imaging techniques show the physiological inhomogeneity of transmural myocardial mechanics. Polarized light imaging makes it possible to quantitatively analyse the myocardial cell orientation to study the regional isotropy of myosin filaments (a new parameter) and to provide virtual dissection (a new tool) of the myocardial ventricular mesh. This deep inside is complementary of superficial anatomical description.Sixteen normal hearts of human term stillbirths, newborns and infants were studied. During the first months of postnatal age, the median regional isotropy values decreased in the ventricular three-dimensional mesh. There was a progressive appearance of a particularly inhomogeneous secondary arrangement of myocardial cells with alternation of thick low-RI and thin high-RI areas. The topology of Torrent-Guasp' cleavage plans and intercalated high RI areas were identical. The outlet septum was constantly identified.Eleven malformed hearts were studied. The deep components of the ventriculo-infundibular fold, the outlet septum and the latitudinal fibres of the ventricular walls were described and 3 different patterns could be portrayed : 1) a normal aligned pattern ; 2) a parallel pattern of the ventriculo-infundibular fold and the outlet septum in Tetralogy of Fallot ; 3) a V pattern of the ventriculo-infundibular fold in heart with mitro-arterial muscular discontinuity.To conclude, we uncover new elements to understand the onset of the myocardial architecture in normal and pathological hearts. However, this new data need to be statistically consolidated by studying a greater numbers of cases. As a perspective, these observations will be confronted to physiological data provided by functional imaging technique
Rosa, Marlise. "SEGMENTAÇÃO DE GRÃOS DE HEMATITA EM AMOSTRAS DE MINÉRIO DE FERRO POR ANÁLISE DE IMAGENS DE LUZ POLARIZADA." Universidade Federal de Santa Maria, 2008. http://repositorio.ufsm.br/handle/1/8064.
Full textThe aim of the present work is to classify co-registered pixels of stacks of polarized light images of iron ore into their respective crystalline grains or pores, thus producing grain segmented images that can be analyzed by their size, shape and orientation distributions, as well as their porosity and the size and morphology of the pores. Polished sections of samples of hematite-rich ore are digitally imaged in a rotating polarizer microscope at varying planepolarization angles. An image stack is produced for every field of view, where each image corresponds to a polarizer position. Any point in the sample is registered to the same pixel coordinates at all images in the stack. The resulting set of intensities for each pixel is directly related to the orientation of the crystal sampled at the corresponding position. Multivariate analysis of the sets of intensities leads to the classification of the pixels into their respective crystalline grains. Individual hematite grains of iron ore, as well as their pores, are segmented. The results are compared to those obtained by visual point counting methods.
O objetivo do presente trabalho é classificar pixels co-registrados de pilhas de imagens de luz polarizada de minério de ferro nos seus respectivos grãos cristalinos ou poros, produzindo assim imagens segmentadas por grãos que podem ser analisados quanto às suas distribuições de tamanho, forma e orientação, bem como sua porosidade, tamanho e forma dos poros. Seções polidas de amostras de minério de ferro rico em hematita foram imageadas difratalmente em um microscópio com polarizador giratório em ângulos variados de polarização. Uma pilha de imagens foi produzida para cada campo na qual cada imagem corresponde a uma orientação do polarizador. Cada ponto na amostra foi registrado nas mesmas coordenadas em todas as imagens da pilha. O conjunto resultante de intensidades de cada pixel está diretamente relacionado com a orientação do cristal amostrado na posição correspondente. A análise multivariada dos conjuntos de intensidades leva à classificação dos pixels nos seus respectivos grãos cristalinos. Grãos individuais de hematita do minério de ferro, bem como os seus poros foram segmentados. Os resultados foram comparados com aqueles obtidos pelo método de contagem dos pontos, ou seja, por inspeção visual.
Georgiev, Georgi Yordanov. "Structural studies of polymers and polymer liquid crystals by X-ray scattering, thermal analysis and ellipsometric studies through polarized light microscopy /." Thesis, Connect to Dissertations & Theses @ Tufts University, 2002.
Find full textAdviser: Peggy Cebe. Submitted to the Dept. of Physics. Includes bibliographical references. Access restricted to members of the Tufts University community. Also available via the World Wide Web;
Polowsky, Patrick. "Analysis of surface crystals on soft washed rind cheeses using polarized light microscopy and their effect on the sensory perception of grittiness." ScholarWorks @ UVM, 2018. https://scholarworks.uvm.edu/graddis/864.
Full textMartinek, Radomír. "Analýza metod pro hodnocení submikrostruktury buněčné stěny dřeva." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2018. http://www.nusl.cz/ntk/nusl-372274.
Full textThomas, Jimmy. "An investigation on the formation and occurrence of spiral grain and compression wood in radiata pine (Pinus radiata D. Don.)." Thesis, University of Canterbury. Biological Sciences, 2014. http://hdl.handle.net/10092/9654.
Full textGwinnett, Claire M. B. "The Use of Inexperienced Personnel in the Analysis of Synthetic Textile Fibres using Polarized Light Microscopy for the Generation of Data Suitable for the Production of a Synthetic Fibres Database." Thesis, Staffordshire University, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522253.
Full textDevaki, Sudha J., Neethu K. Sadanandhan, Renjith Sasi, Hans-Juergen P. Adler, and Andrij Pich. "Water dispersible electrically conductive poly(3,4- ethylenedioxythiophene) nanospindles by liquid crystalline template assisted polymerization." Royal Society of Chemistry, 2014. https://tud.qucosa.de/id/qucosa%3A36259.
Full textMaside, Mielgo Carolina. "Efecto de diversas técnicas para visualizar la placa metafásica y el corpúsculo polar sobre la capacidad de desarrollo de ovocitos porcinos madurados in vitro." Doctoral thesis, Universidad de Murcia, 2012. http://hdl.handle.net/10803/104603.
Full textSomatic cell nuclear transfer (SCNT) technology in porcine has become a very useful tool for the elaboration of genetic models for human diseases and the use in xenotransplantation. The efficiency of SCNT is still very low, although the number of cloned pigs increases each year. One of the hardest steps of porcine SCNT is the enucleation of the oocyte because its cytoplasm contains many lipid droplets. The main objective of this thesis was to assess the effect of several approaches to visualize the metaphase II plate and the first polar body on the developmental ability of in vitro mature porcine oocytes.
Novák, Kamil. "Analýza vlivu uspořádání kolagenu na mechanické vlastnosti tepen." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2018. http://www.nusl.cz/ntk/nusl-383532.
Full textvan, Dijk Jeroen. "Size and Abundance of Late Pleistocene Reticulofenestrid Coccoliths from the Eastern Indian Ocean in Relation to Temperature and Aridity." Thesis, Uppsala universitet, Institutionen för geovetenskaper, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-325273.
Full textMätningar av abundans och massa hos coccoliter kan användas som en signal för primärproduktion och pelagisk förkalkning som resultat av miljöförändringar. Leeuwin Current (LC) är känd för att transportera varmt vatten och vatten med låg salthalt från Indo-Pacific Warm Pool (IPWP) söderut längs kusten i västra Australien. Tillsammans med början av kontinental torka under sen Neogen kan ökad styrka hos LC ha spelat en roll i expansionen av rev på nordvästsockeln. I denna studie bedömdes den morfologiska variationen i storlek och massa hos coccoliter i material från IODP plats U1461 i östra Indiska oceanen från de senaste 500 000 åren. Både den absoluta abundansen av alla reticulofenstridcoccoliter (Emiliania huxleyi, Reticulofenestra spp., Gephyrocapsa spp. och Pseudoemiliania spp.) bestämdes, liksom den relativa abundansen av stora jämfört med små coccoliter. Storlek och massa av coccoliter mättes kvantitativt under cirkulärt polariserat ljus. Uppgifterna jämfördes med variationer i havsytans temperatur (SST) hos LC, och med kontinental torrhet i Australien. SST-fluktuationer kan påverka produktiviteten hos coccolitoforider genom att påverka deras metabolism, medan kontinental torrhet kan påverka inflödet av markmaterial med vind. Det undersökta intervallet domineras av små arter av Gephyrocapsa. Toppvärden av absolut abundans och massa observerades under marinisotopsteget (MIS) 11, en interglacial period med förlängd värme och fuktighet. Dessa resultat kombinerat med hög densitet av aragonitnålar i samma prover indikerar att sedimenten späddes ut med material som svämmade över från den intilliggande grunda karbonatplattformen, vilket är jämförligt med de vitningshändelser som har observerats i dagens Bahamas. En minskning i abundans av Gephyrocapsa caribbeanica vid 240 ka kan kopplas till tidpunkten för deras senaste gemensamma förekomst (LCO) inom MIS 7. Den efterföljande övergången till Gephyrocapsa oceanica som den dominerande stora arten kan indikera en ekologisk ersättning av G. caribbeanica, eller indikera varmt vatten med låg salthalt.
Dumont, George Pierre Jr. "The use of carbonation and fractional evaporative crystallization in the pretreatment of Hanford nuclear wastes." Thesis, Atlanta, Ga. : Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/24716.
Full textCommittee Chair: Dr. Ronald W. Rousseau; Committee Member: Dr. Amyn S, Teja; Committee Member: Dr. Wm. James Frederick Jr.
Relaix, Sabrina. "Cristaux liquides cholestériques en conditions non usuelles de photogélification : modulation du profil de réflexion de la lumière." Phd thesis, Université Paul Sabatier - Toulouse III, 2007. http://tel.archives-ouvertes.fr/tel-00179999.
Full textLes travaux de thèse se sont focalisés sur l'obtention de propriétés de réflexion de cholestériques gélifiés atypiques, avec deux objectifs de recherche: l'élargissement de la bande de réflexion et l'accroissement de la quantité de lumière réfléchie.
La première partie du manuscrit de thèse propose un procédé d'élaboration permettant un élargissement de la bande de réflexion de gels de CLC. Cet objectif est atteint par l'utilisation d'un CLC absorbant la lumière UV, à l'origine d'un gradient d'intensité lors de la gélification. Des études par microscopie optique, spectrophotométrie et microscopie électronique en transmission ont permis de déterminer la distribution structurale à l'origine de la distribution des longueurs d'onde de réflexion observée.
La seconde partie du manuscrit se focalise sur l'augmentation de la quantité de lumière réfléchie par un gel réalisé à partir d'un mélange cholestérique qui voit son sens d'hélicité changer avec la température. L'impact de l'histoire de la polymérisation et du parcours en température après gélification sur les propriétés du profil de réflexion sera analysé avec une attention particulière sur la nature et la quantité de la polarisation du faisceau réfléchi.
Woldemichael, Michael Haile. "The Mineralogical Composition of House Dust in Ontario, Canada." Thesis, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/20664.
Full textBayuadri, Cosmas. "Stability of sodium sulfate dicarbonate (~2Na₂CO₃• Na₂SO₄) crystals." Thesis, Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/11507.
Full textSeltmann, Jens. "Struktur-Eigenschaftsbeziehungen V-förmiger Mesogene zur Realisierung biaxial nematischer Mesophasen." Doctoral thesis, Universitätsbibliothek Chemnitz, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:ch1-qucosa-67693.
Full textHuang, Yung-Kuo, and 黃永國. "Inspection of 3D Profile and Internal Structure with Polarized Low Coherent Light Interference Microscope." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/93011580853076290987.
Full text國立中正大學
機械工程所
94
Abstract In this paper, we set up a polarized sensitivity optical coherence tomography microscope (PS-OCM) of Linnik type. This PS-OCM was modified mechanism of the reference arm in order to compensate the difference of optical path. So the microscope can achieve the function of white light interference microscope (WLI), virtual confocal microscope and the PS-OCM. The resolution of WLI reached to nanometers scale. So it is indispensable to MENS or semiconductor manufacture procedure. The optical tomography had the functions which was transparent material internal structure measured and bio-tissue internal imaging. Therefore, it was increasingly attach importance to field of optical inspection except field of biotechnology. Additionally, OCM will inspect the birefringence, when the OCM used the polarization light source. Inspection of 3D profile with this PS-OCM, we developed a new scanning algorithm. This algorithm combined Hilbert transform to search the zero order fringe of the interferogram. And measure a 3D profile standard to prove the correctness of the algorithm. The PS-OCM can execute the function of microscope, and this paper develops a virtual confocal algorithm. Use this algorithm, the PS-OCM except inspected 3D profile and provide the full depth of field image of the sample. The PS-OCM can measure the internal structure of transparent material which operated with the mechanism of optical difference compensated. And the paper drove an equation of optical difference compensated which regulated the displacement of the compensation mechanism. Because, the sample arm of the PS-OCM would be longer when focus fall in transparent material internal. So, this PS-OCM is unrestricted with the depth of field of objective on the depth of measurement. Finally, we demonstrate a measurement of the stress induce birefringence with the PS-OCM.
Chen, Yueh-Hsiang, and 陳躍翔. "Collection and Polarized Light Microscopy Analysis of Textile Fiber Evidence in Traffic Accidents." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/h82xhk.
Full text中央警察大學
鑑識科學研究所
99
Crime scene investigators collect and preserve evidences in major criminal cases, except for homicide, robbery, and sexual assault cases attracting public attention, also playing important roles in traffic accidents investigation, especially in traffic homicide cases. To effectively collect accident evidence, investigators must thoroughly understand the dynamic relationships from pre-accident to post-accident between the vehicle and the victim, vehicle damage, evidence left patterns and locations, victim’s traumatic injuries, and clothes abrasions and lacerations. In this study, 22 cases were studied to get a better strategy for forensic traffic accident textile fiber evidence search and collection. Traffic accidents involved pedestrians and scooter riders can be classified into 4 categories. Forensic fiber analysis by polarized light microscopy (PLM) usually re-fers to observation refractive index, extinction, interference colors, birefringence and other physical properties under the polarized light micro-scope to determine fiber generic types. In this study, retardations of textile fiber specimens were measured with Berek compensator under the polarized light microscope. This method improves the accuracy and digitalizes the result of interference colors observation with true retardation. A datasheet for forensic textile fiber identification with polarized light microscopy is proposed, and the flowchart system is theorized. The datasheet and flowchart system allow forensic textile fiber examiners to distinguish and identify common synthetic fibers encountered in caseworks.
Quackenbush, Brett Michael 1969. "A comparison of a 2.26% fluoride varnish versus a 1.23% APF foam using polarized light microscopy, confocal microscopy and quantitative light fluorescence." Thesis, 2000. http://hdl.handle.net/1805/3712.
Full textSecondary caries and the replacement of existing restorations account for 50 to 70 percent of operative dentistry today. Quantitative Light Fluorescence (QLF) has been shown to be effective at diagnosing very early tooth demineralization on smooth surfaces (less than 50 μ in depth); however, QLF has never been utilized to evaluate secondary caries in dentin. The objective of this study was to validate the accuracy of QLF in diagnosing early secondary caries and then verify the results using confocal microscopy and polarized light microscopy. Seventy-five mandibular molar teeth were prepared with Class V amalgam preparations on the mesial surface. A fluoridated varnish and 1.23- percent acidulated phosphate fluoride (APF) were introduced to this evaluation system, two agents known to effectively inhibit tooth demineralization. The artificial caries system utilized was adjusted to ensure that secondary caries would occur at restoration/tooth surface interfaces. The teeth were exposed to this artificial caries challenge for five days and following lesion formation, QLF was used to determine if incipient demineralization could be detected. The results of the QLF analysis were then compared with the data gathered using confocal microscopy and polarized light microscopy. Our results demonstrate that QLF detected 100 percent of the lesions seen with confocal microscopy and polarized light microscopy; however, no sound specimens were analyzed with any of the three techniques. There were no consistent significant differences between the fluoridated varnish and APF (p < 0.05) with any of the three methods utilized. We conclude that QLF can be used in early caries diagnosis and that emphasis should now be focused on treatment of the early lesion.
Godinho, Isabel Ribeiro. "Structure and stability of creams containing ectoine: liquid crystalline states." Master's thesis, 2016. http://hdl.handle.net/10451/35849.
Full textEctoine is a low molecular organic osmolyte, produced by various extremolytes, which have a large range of applications, such as medical devices, cosmetics, and life science products, due to their ability to protect biological macromolecules and cells from damage by external stresses. The aim of this project was to study the influence that ectoine can have on the properties of creams. This substance has several positive effects when used in dermocosmetic products, however it is thought that ectoine can possibly change the stability of creams. To study this possibility, simple creams constituted by different lyotropic liquid crystalline phases, such as lamellar, hexagonal and cubic phase; and more complex creams, such as anionic and non-ionic hydrophilic creams, were used. During the project, it was shown that ectoine added on creams could provoke various alterations in lyotropic liquid crystalline phases, such as the transition between mesophases and the change of the creams’ viscosity. Therefore, it was proved, by using the polarized light microscopy and rheological measurements, that ectoine could change the structure and stability of creams to a certain extent, as expected.
A ectoina é um osmólito orgânico de baixo peso molecular, produzido por diversos extremólitos, os quais têm uma grande variedade de aplicações, tais como dispositivos médicos, cosméticos e produtos de científicos, devido ao seu mecanismo de autoproteção, protegendo as células e as macromoléculas biológicas das condições ambientais extremas. O objetivo deste trabalho foi estudar a influência que a ectoina poderia ter nas propriedades dos cremes. Esta substância tem vários efeitos positivos quando utilizada em produtos de dermocosmética, no entanto pensa-se que a ectoina pode, possivelmente, mudar a estabilidade de cremes. Para estudar esta possibilidade foram usados cremes simples constituídos por diferentes fases de cristais líquidos liotrópicos, fase lamelar, hexagonal e cúbica; e cremes mais complexos, tais como, cremes hidrofílicos aniónicos e não-iónicos. Durante o projeto, foi demonstrado que a ectoina ao ser adicionada em cremes, pode provocar várias alterações nas fases de cristais líquidos liotrópicos, tais como a transição entre mesofases e a alteração da viscosidade dos cremes. Assim, provou-se, utilizando a microscopia de luz polarizada e medições reológicas, que a ectoina poderia alterar a estrutura e estabilidade de cremes, em certa medida, como esperado.
Raguin, Emeline. "Histomorphometric assessment of double-zonal osteons in human cortical bone." Thèse, 2018. http://hdl.handle.net/1866/21121.
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