Relevant bibliographies by topics / Pneumatically assisted desorption/ionization

Academic literature on the topic 'Pneumatically assisted desorption/ionization'

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Published: 11 March 2023

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Journal articles on the topic "Pneumatically assisted desorption/ionization"

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Duncan, Kyle D., Hilde-Marléne Bergman, and Ingela Lanekoff. "A pneumatically assisted nanospray desorption electrospray ionization source for increased solvent versatility and enhanced metabolite detection from tissue." Analyst 142, no. 18 (2017): 3424–31. http://dx.doi.org/10.1039/c7an00901a.

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Mavroudakis, Leonidas, and Ingela Lanekoff. "Ischemic Stroke Causes Disruptions in the Carnitine Shuttle System." Metabolites 13, no. 2 (February 14, 2023): 278. http://dx.doi.org/10.3390/metabo13020278.

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Gaining a deep understanding of the molecular mechanisms underlying ischemic stroke is necessary to develop treatment alternatives. Ischemic stroke is known to cause a cellular energy imbalance when glucose supply is deprived, enhancing the role for energy production via β-oxidation where acylcarnitines are essential for the transportation of fatty acids into the mitochondria. Although traditional bulk analysis methods enable sensitive detection of acylcarnitines, they do not provide information on their abundances in various tissue regions. However, with quantitative mass spectrometry imaging the detected concentrations and spatial distributions of endogenous molecules can be readily obtained in an unbiased way. Here, we use pneumatically assisted nanospray desorption electrospray ionization mass spectrometry imaging (PA nano-DESI MSI) doped with internal standards to study the distributions of acylcarnitines in mouse brain affected by stroke. The internal standards enable quantitative imaging and annotation of endogenous acylcarnitines is achieved by studying fragmentation patterns. We report a significant accumulation of long-chain acylcarnitines due to ischemia in brain tissue of the middle cerebral artery occlusion (MCAO) stroke model. Further, we estimate activities of carnitine transporting enzymes and demonstrate disruptions in the carnitine shuttle system that affects the β-oxidation in the mitochondria. Our results show the importance for quantitative monitoring of metabolite distributions in distinct tissue regions to understand cell compensation mechanisms involved in handling damage caused by stroke.
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Lu, I.-Chung, Chuping Lee, Yuan-Tseh Lee, and Chi-Kung Ni. "Ionization Mechanism of Matrix-Assisted Laser Desorption/Ionization." Annual Review of Analytical Chemistry 8, no. 1 (July 22, 2015): 21–39. http://dx.doi.org/10.1146/annurev-anchem-071114-040315.

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Liu, Bo-Hong, Oleg P. Charkin, Nina Klemenko, Chiu Wen Chen, and Yi-Sheng Wang. "Initial Ionization Reaction in Matrix-Assisted Laser Desorption/Ionization." Journal of Physical Chemistry B 114, no. 33 (August 26, 2010): 10853–59. http://dx.doi.org/10.1021/jp104178m.

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Dattelbaum, Andrew M., and Srinivas Iyer. "Surface-assisted laser desorption/ionization mass spectrometry." Expert Review of Proteomics 3, no. 1 (February 2006): 153–61. http://dx.doi.org/10.1586/14789450.3.1.153.

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Michalak, Leszek, Keith J. Fisher, David S. Alderdice, Daniel R. Jardine, and Gary D. Willett. "C60-assisted laser desorption-ionization mass spectrometry." Organic Mass Spectrometry 29, no. 9 (September 1994): 512–15. http://dx.doi.org/10.1002/oms.1210290912.

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Özdemir, Abdil, and Chung-Hsuan Chen. "Electrode-assisted desorption electrospray ionization mass spectrometry." Journal of Mass Spectrometry 45, no. 10 (September 20, 2010): 1203–11. http://dx.doi.org/10.1002/jms.1815.

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Karbach, Volker, Richard Knochenmuss, and Renato Zenobi. "Matrix-assisted filament desorption/ionization mass spectrometry." Journal of the American Society for Mass Spectrometry 9, no. 11 (November 1998): 1226–28. http://dx.doi.org/10.1016/s1044-0305(98)00099-3.

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Karas, Michael, Ute Bahr, and Ulrich Gießmann. "Matrix-assisted laser desorption ionization mass spectrometry." Mass Spectrometry Reviews 10, no. 5 (September 1991): 335–57. http://dx.doi.org/10.1002/mas.1280100503.

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Tarka, Mark. "Is laser desorption/ionization TOFMS field-assisted?" Rapid Communications in Mass Spectrometry 13, no. 10 (May 30, 1999): 975. http://dx.doi.org/10.1002/(sici)1097-0231(19990530)13:10<975::aid-rcm582>3.0.co;2-q.

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Dissertations / Theses on the topic "Pneumatically assisted desorption/ionization"

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ZANCANARO, Flavio. "Mass spectrometry pneumatically assisted desorption/ionization in forensic toxicology." Doctoral thesis, Università degli Studi di Verona, 2010. http://hdl.handle.net/11562/342863.

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La spettrometria di massa è una delle tecniche più rilevanti in tossicologia clinica e forense. Il suo sviluppo e il miglioramento si basano sull'invenzione e l'uso di nuove sorgenti di ioni, nuovi metodi di ionizzazione, nuovi analizzatori di massa e nuove tecniche di pre-trattamento dei campioni. Una recente innovazione è la capacità di registrare spettri di massa su campioni reali direttamente nel loro ambiente nativo, senza preparazione del campione o pre-separazione. In questo ambito è stato descritto un nuovo metodo di ionizzazione/desorbimento chiamato DESI (desorbimento Electrospray ionizzazione), in seguito è stato sviluppato un metodo chiamato Dessì (Desorbimento Sonic Spray ionizzazione), a prima vista simile a DESI, ma in fondo sostanzialmente diverso. Questa tesi consiste nello sviluppo di una nuova interfaccia di desorbimento / ionizzazione per indagare il vero meccanismo coinvolto nella formazione di ioni, perché abbiamo ritenuto questo passaggio propedeutico per garantire il successivo uso del metodo in campo tossicologico analitico. Abbiamo verificato che il contributo pneumatico è preponderante per ottenere risultati. Quindi, la nostra nuova interfaccia di desorbimento/ionizzazione utilizza solo uno spray di solvente puro, senza alcuna tensione elettrica applicata allo sprayer. Un aspetto fondamentale di questo progetto, applicato a diverse matrici complesse, è il numero di parametri di funzionamento controllabili che possono essere studiati e ottimizzati per ottenere un'efficace analisi di superficie. Le variabili più importanti prese in considerazione sono state la geometria della sorgente (l'angolo di spray e l'angolo di diffusione di ioni, come pure le varie distanze nell'allineamento dello spray, del campione e dello spettrometro di massa) e la caratteristica dello spray (il contenuto del solvente ed il gas di portata). Tutte le misurazioni sono state eseguite in condizioni di ionizzazione positiva e negativa, variando tensione del capillare, pressione del gas di nebulizzazione, il flusso di gas al capillare e la temperatura del capillare. L'acquisizione è stata prodotta in modalità multiple mass spectra (MSn). Abbiamo applicato questa nuova soluzione tecnica per l'identificazione di composti tal quali, di principi attivi in campioni di farmaci mediante analisi diretta di compresse, principi attivi contenuti in specie vegetali. Gli sviluppi futuri saranno connessi ad applicare l’analisi diretta di analiti presenti sulle superfici originali di interesse nel settore tossicologico per il campionamento in vivo su superfici di tessuti, per individuare l’esposizione a farmaci e xenobiotici, oltre alla possibilità di costruire un’immagine chimica della distribuzione spaziale di analiti sulle superfici dei campioni.
Mass spectrometry is one of the most relevant techniques in clinical and forensic toxicology. Its development and improvement are based on the invention and utilization of new ion sources, new ionization methods, new mass analyzers and new sample pre-treatment techniques. A recent innovation is the ability to record mass spectra on ordinary samples in their native environment, without sample preparation or pre-separation. In this field, a new desorption ionization method called DESI (Desorption Electrospray Ionization) has been described; subsequently, method called DeSSI (Desorption Sonic Spray Ionization), at first sight similar to DESI, but in deep substantially different, has been developed. This thesis consist in developing a new desorption/ionization interface to investigate the real mechanism involved in ions formation because we considered that propaedeutic for the extensive use of the method in the toxicological analytical field. We verified that the pneumatic contribution is preponderant to the obtained results. Hence, our new desorption/ionization interface uses only a spray of pure solvent with no high voltage on needle. A key aspect of this project, applied to several complex matrix, is the number of controllable operating parameters that can be investigated and optimized to obtain an efficient surface analysis. The most important variables are taken in consideration were the source geometry (the spray angle and the ion uptake angle, as well as the various distances in aligning the spray, sample and mass spectrometer) and the characteristic of sprayer (contents of the solvent spray and gas flow rate). All measurements have been performed in positive and negative ionization conditions, varying capillary voltage, nebulizing gas pressure, drying gas flow and end plate temperature. Acquisition was in multiple mass spectrometry mode (MSn). 2 We have applied this new technical solution to compound identification, active principles and drugs identification in direct tablet analysis, active principles and drugs identification in vegetable species. Future developments will be related to apply the direct analysis of analytes present on the original surfaces of interest in the toxicological field for in vivo sampling of living tissue surfaces, to identify drug and xenobiotic exposure, besides the chemical imaging of spatial distribution of analytes onto sample surfaces.
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Peng, Ivory Xingyu. "Electrospray-assisted laser desorption ionization mass spectrometry for proteomic studies." Diss., Restricted to subscribing institutions, 2009. http://proquest.umi.com/pqdweb?did=1997571271&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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Berhane, Beniam T. "Rapid Characterization of Posttranscriptional Modifications in RNA Using Matrix Assisted Laser Desorption Ionization Mass Spectrometry and Matrix Assisted Laser Desorption Ionization Post Source Decay Mass Spectrometry." University of Cincinnati / OhioLINK, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1052319621.

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Dubois, Frédéric Dubois Frédéric. "Ion formation and detection in matrix-assisted laser desorption/ionization mass spectrometry /." [S.l.] : [s.n.], 1999. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=13255.

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Dai, Yuqin. "Development of matrix-assisted laser desorption ionization mass spectrometry for biopolymer analysis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0003/NQ39519.pdf.

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Tummala, Manorama. "Surfactant-Aided Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (SA-MALDI MS)." University of Cincinnati / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1100672049.

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Dashtiev, Maxim. "Fluorescence spectroscopy of trapped molecular ions produced with matrix-assisted laser desorption/ionization /." Zürich : ETH, 2006. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=16949.

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Segu, Mohideen Mohamed Zaneer. "TARGET MODIFICATION FOR ENHANCED PERFORMANCE MATRIX ASSISTED LASER DESORPTION IONIZATION (MALDI) MASS SPECTROMETRY." Available to subscribers only, 2008. http://proquest.umi.com/pqdweb?did=1674093101&sid=1&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Thesis (Ph. D.)--Southern Illinois University Carbondale, 2008.
"Department of Chemistry." Keywords: Enhanced MALDI, MALDI-MS, On-probe separation, Protein-surface interactions, Sublayers, Surface binding capacity. Includes bibliographical references (p. 130-148). Also available online.
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Akinapalli, Srikanth. "MICROFLUIDIC DYNAMIC ISOELECTRIC FOCUSING COUPLED TO MATRIX ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY." OpenSIUC, 2016. https://opensiuc.lib.siu.edu/dissertations/1289.

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Proteomics is an increasingly important area of biological research and has gathered much attention over recent years. Major challenges that make a proteomic analysis difficult are sample complexity, diversity and dynamic range. Progress in the area of proteomics relies heavily on new analytical tools for the sensitive, selective, and high-throughput studies of target analytes. It is estimated that there are several hundred thousand proteins in a human cell. In order to be able to analyze such a complex sample, an analytical method must be capable of separating and detecting many different sample peaks. The complexity of such samples indicates that a single separation method will not be able to provide the needed resolution. If two methods that are orthogonal are combined, then the peak capacity of the combined system is the product of the two individual peak capacities. Development of such systems would cater to the current demands of proteomics studies. Matrix assisted laser desorption/ionization (MALDI) mass spectrometry has evolved into a primary analytical tool for proteomics research. MALDI is fast and efficient and has a high tolerance to non-volatile buffers and impurities. The samples for MALDI are typically applied to solid supports after having been subjected to off-line liquid or gel separations. Several methods have been reported involving various chromatographic or electrophoretic separation methods. However, the current methods often require highly sophisticated sample handling systems, which are often expensive and in need of skilled human resources. The current demands of proteomic analyses require fast, efficient and inexpensive methods for separation to fully harness the capability of MALDI mass spectrometry. In this work a microfluidic device has been designed to perform dynamic isoelectric focusing (DIEF) based protein separation with digital sample deposition directly on a MALDI target for offline analysis. DIEF is related to capillary isoelectric focusing which and can facilitate the interface without the loss of the separation resolution. Compared to traditional capillary isoelectric focusing (cIEF) DIEF uses additional high-voltage power supplies to control the pH gradient by manipulating the electric field. The proteins can be focused at a desired sampling position according to their isoelectric point, to be collected for further analysis by MALDI mass spectrometry. DIEF has a peak capacity of over a thousand and offers an ease of interfacing to other techniques making it a preferred separation method for the interface with mass spectrometric techniques such as MALDI. The design of the microfluidic device is based on a digital droplet fractionation. Multiple fractions of the sample solution from DIEF are generated to retain the resolution and to act as an additional separation mode. The microfluidic device is controlled by actuating pneumatic valves built into the device. The DIEF operational parameters were optimized according to the surface functionality and the design of the microfluidic device. A suitable MALDI sample preparation method was found by studying different existing methods. The methods were studied using test proteins prepared in solutions having the additives used in the experiment. A simple mixture of three proteins was used to demonstrate the application of the developed method. The separation between the proteins insulin, hemoglobin and the myoglobin was demonstrated by varying the separation resolution in three experiments.
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Xiang, Fan. "Improvement and investigation of sample preparation for matrix-assisted laser desorption/ionization of proteins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25780.pdf.

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Books on the topic "Pneumatically assisted desorption/ionization"

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Liang, Li, ed. MALDI mass spectrometry for synthetic polymers analysis. Hoboken: Wiley, 2010.

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Kopecký, Dušan. Deposition of polypyrrole thin films by advanced method: Matrix assisted pulsed laser evaporation. Hauppauge, N.Y: Nova Science Publishers, 2011.

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Peptidomics: Methods and protocols. New York, NY: Humana Press, 2010.

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1948-, Vivanco Fernando, ed. Cardiovascular proteomics: Methods and protocols. Totowa, N.J: Humana Press, 2007.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Incorporated, John, 2013.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Limited, John, 2007.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Incorporated, John, 2013.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Limited, John, 2013.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Incorporated, John, 2013.

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Maldi MS: A Practical Guide to Instrumentation, Methods, and Applications. Wiley-VCH Verlag GmbH, 2013.

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Book chapters on the topic "Pneumatically assisted desorption/ionization"

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Gross, Jürgen H. "Matrix-Assisted Laser Desorption/Ionization." In Mass Spectrometry, 507–59. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10711-5_11.

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Gross, Jürgen H. "Matrix-Assisted Laser Desorption/Ionization." In Mass Spectrometry, 411–40. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/3-540-36756-x_10.

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Morgan, Michael M., MacDonald J. Christie, Thomas Steckler, Ben J. Harrison, Christos Pantelis, Christof Baltes, Thomas Mueggler, et al. "Matrix-Assisted Laser Desorption Ionization." In Encyclopedia of Psychopharmacology, 751. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_1549.

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Satoh, Takaya. "Matrix-Assisted Laser Desorption/Ionization." In Compendium of Surface and Interface Analysis, 365–69. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-6156-1_60.

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Gross, Jürgen H. "Matrix-Assisted Laser Desorption/Ionization." In Mass Spectrometry, 651–720. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-54398-7_11.

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Abdelhamid, Hani Nasser. "Nanoparticles Assisted Laser Desorption/Ionization Mass Spectrometry." In Handbook of Smart Materials in Analytical Chemistry, 729–55. Chichester, UK: John Wiley & Sons, Ltd, 2019. http://dx.doi.org/10.1002/9781119422587.ch23.

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Waki, Michihiko, Eiji Sugiyama, Takeshi Kondo, Keigo Sano, and Mitsutoshi Setou. "Nanoparticle-Assisted Laser Desorption/Ionization for Metabolite Imaging." In Methods in Molecular Biology, 159–73. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1357-2_16.

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Chait, Brian T., and Ronald C. Beavis. "Matrix-assisted laser desorption and ionization of biomolecules." In Laser Ablation Mechanisms and Applications, 149–53. New York, NY: Springer New York, 1991. http://dx.doi.org/10.1007/bfb0048364.

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Fournelle, Frédéric, and Pierre Chaurand. "Metal-Assisted Laser Desorption Ionization Imaging Mass Spectrometry." In Methods in Molecular Biology, 99–115. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-2030-4_7.

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Mathur, Sonal, Alexis Nazabal, and Renato Zenobi. "Probing Noncovalent Interactions by Electrospray Ionization and Matrix-Assisted Laser Desorption/Ionization." In Electrospray and MALDI Mass Spectrometry, 535–70. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9780470588901.ch15.

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Conference papers on the topic "Pneumatically assisted desorption/ionization"

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Murray, Kermit K., and David H. Russell. "Matrix-assisted laser desorption ionization of aerosols: The ionization mechanism." In Laser ablation: mechanisms and applications—II. AIP, 1993. http://dx.doi.org/10.1063/1.44843.

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Kinsel, Gary R., Kent Gillig, Ricky Edmondson, and David H. Russell. "Fundamental Investigations of the Mechanism of Laser Desorption and Ionization in Matrix Assisted Laser Desorption / Ionization." In Laser Applications to Chemical Analysis. Washington, D.C.: Optica Publishing Group, 1994. http://dx.doi.org/10.1364/laca.1994.thb.1.

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The recent development of Matrix Assisted Laser Desorption / Ionization (MALDI) has sparked a revolution in the field of high molecular weight mass spectrometry.1 Time-of-flight (TOF) mass spectra of proteins weighing up to 300,000 Da are now routinely produced and this achievement has fostered a variety of bioanalytical applications which were previously unapproachable using conventional mass spectrometric techniques. These successful applications have burgeoned in spite of a poor understanding of the mechanism of analyte desorption and ionization under MALDI conditions. An improved understanding of the MALDI mechanism should aid in overcoming a number of limitations of the current state-of-the-art and forms the motivation for the work described.
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Karas, Michael, and Franz Hillenkamp. "Matrix-assisted laser desorption ionization mass spectrometry-fundamentals and applications." In Laser ablation: mechanisms and applications—II. AIP, 1993. http://dx.doi.org/10.1063/1.44841.

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Flanigan, Paul, Fengjian Shi, Jieutonne Archer, Andrew Mills, Martin Fermann, and Robert Levis. "High Repetition-Rate, Pulse-Burst Assisted Desorption, Electrospray Post-Ionization Mass Spectrometry." In CLEO: Applications and Technology. Washington, D.C.: OSA, 2014. http://dx.doi.org/10.1364/cleo_at.2014.aw1l.5.

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Castoro, John A., and Charles L. Wilkins. "Fourier-transform mass spectrometry for high-resolution matrix-assisted laser desorption/ionization MS." In OE/LASE'93: Optics, Electro-Optics, & Laser Applications in Science& Engineering, edited by Mattanjah S. de Vries. SPIE, 1993. http://dx.doi.org/10.1117/12.148521.

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Tummala, Rama, Lisa M. Ballard, Gary A. Breaux, Kari B. Green-Church, and Patrick A. Limbach. "Investigation into surfactant-aided matrix-assisted laser desorption/ionization-mass spectrometry for peptide mapping." In BiOS 2000 The International Symposium on Biomedical Optics, edited by Patrick A. Limbach, John C. Owicki, Ramesh Raghavachari, and Weihong Tan. SPIE, 2000. http://dx.doi.org/10.1117/12.380494.

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Vertes, Akos, Akos Bencsura, Mehrnoosh Sadeghi, and Xiongwu Wu. "Adduct formation and energy redistribution in UV and IR matrix-assisted laser desorption ionization." In Symposium on High-Power Lasers and Applications, edited by Richard F. Haglund, Jr. and Richard F. Wood. SPIE, 2000. http://dx.doi.org/10.1117/12.380793.

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Hurst, Gregory B., Robert L. Hettich, M. V. Buchanan, and Elizabeth A. Stemmler. "Matrix-assisted laser desorption/ionization mass spectrometry for the structural characterization of modified oligonucleotides." In Laser ablation: mechanisms and applications—II. AIP, 1993. http://dx.doi.org/10.1063/1.44851.

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Schriemer, David, Yuqin Dai, and Liang Li. "Effect of sample compositions on chemical analysis using matrix-assisted laser desorption ionization mass spectrometry." In SPIE's 1996 International Symposium on Optical Science, Engineering, and Instrumentation, edited by Tuan Vo-Dinh. SPIE, 1996. http://dx.doi.org/10.1117/12.259762.

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Karas, Michael, Klaus Dreisewerd, Martin Schürenberg, Bing Wang, and Franz Hillenkamp. "Matrix-assisted laser desorption ionization and why a second laser for gas phase excitation may be a useful addition." In The 7th international symposium: Resonance ionization spectroscopy 1994. AIP, 1995. http://dx.doi.org/10.1063/1.47556.

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Reports on the topic "Pneumatically assisted desorption/ionization"

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Young, Steven. Evaluation of the Polymer Characterization Capabilities of Matrix-Assisted Laser Desorption/Ionization (Literature Review). Office of Scientific and Technical Information (OSTI), June 2021. http://dx.doi.org/10.2172/1798113.

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Korte, Andrew R. Development of matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) for plant metabolite analysis. Office of Scientific and Technical Information (OSTI), December 2014. http://dx.doi.org/10.2172/1226566.

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Eades, D., D. Wruck, and H. Gregg. Fundamental studies of matrix-assisted laser desorption/ionization, using time-of-flight mass spectrometry to identify biological molecules. Office of Scientific and Technical Information (OSTI), November 1996. http://dx.doi.org/10.2172/491767.

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Yagnik, Gargey B. Nanoparticle-assisted laser desorption/ionization mass spectrometry: Novel sample preparation methods and nanoparticle screening for plant metabolite imaging. Office of Scientific and Technical Information (OSTI), February 2016. http://dx.doi.org/10.2172/1342543.

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