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Journal articles on the topic 'PMOR'

Author: Grafiati

Published: 4 June 2021

Last updated: 20 February 2023

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1

Fiorani, Mara, and Augusto Accorsi. "Dietary flavonoids as intracellular substrates for an erythrocyte trans-plasma membrane oxidoreductase activity." British Journal of Nutrition 94, no. 3 (September 2005): 338–45. http://dx.doi.org/10.1079/bjn20051504.

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The plasma membrane oxidoreductase (PMOR) activity, which mainly utilises ascorbate as intracellular electron donor, represents a major mechanism for cell-dependent reduction of extracellular oxidants and might be an important process used by the erythrocytes to keep a reduced plasma environment. We previously reported that in human erythrocytes, myricetin and quercetin act as intracellular substrates of a PMOR showing a novel mechanism whereby these flavonoids could exert beneficial effects under oxidative stress conditions. Here, we evaluated the ability of different flavonoids (quercetin, myricetin, morin, kaempferol, fisetin, catechin, luteolin, apigenin, acacetin, rutin, taxifolin, naringenin, genistein) and of two in vivoO-methylated metabolites of quercetin (isorhamnetin and tamarixetin) to be substrates of PMOR, by comparing their antioxidant capacity (i.e. direct interaction with the oxidant ferricyanide or with the free radical 1,1-diphenyl-2-picryl-hydrazil) with their ability to penetrate the erythrocytes and donate electrons to the PMOR. The results obtained indicate that, although most of the flavonoids display significant antioxidant activities, only those (quercetin, myricetin, fisetin) that combine the cathecol structure of the B ring (responsible for the reducing activity) with the 2,3 double bond and 4-oxo function of the C ring (responsible for the uptake by erythrocytes) can act as intracellular substrates for PMOR. It is of note that the metabolites of quercetin enter erythrocytes and donate electrons to the PMOR as the parent compound. The present data show a relationship between the flavonoid structures and their ability to provide electrons to the PMOR, suggesting an additional mechanism whereby dietary flavonoids may exert beneficial effects in man.

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Valsamakis, Alexandra, Paul G. Auwaerter, Bert K. Rima, Hideto Kaneshima, and Diane E. Griffin. "Altered Virulence of Vaccine Strains of Measles Virus after Prolonged Replication in Human Tissue." Journal of Virology 73, no. 10 (October 1, 1999): 8791–97. http://dx.doi.org/10.1128/jvi.73.10.8791-8797.1999.

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ABSTRACT To understand the molecular determinants of measles virus (MV) virulence, we have used the SCID-hu thymus/liver xenograft model (SCID-hu thy/liv) in which in vivo MV virulence phenotypes are faithfully duplicated. Stromal epithelial and monocytic cells are infected by MV in thymus implants, and virulent strains induce massive thymocyte apoptosis, although thymocytes are not infected. To determine whether passage of an avirulent vaccine strain in human tissue increases virulence, we studied a virus isolated from thymic tissue 90 days after infection with the vaccine strain Moraten (pMor-1) and a virus isolated from an immunodeficient child with progressive vaccine-induced disease (Hu2). These viruses were compared to a minimally passaged wild-type Edmonston strain (Ed-wt) and the vaccine strain Moraten. pMor-1, Hu2, and Ed-wt displayed virulent phenotypes in thymic implants, with high levels of virus being detected by 3 days after infection (105.2, 102.8, and 103.4, respectively) and maximal levels being detected between 7 and 14 days after infection. In contrast, Moraten required over 14 days to grow to detectable levels. pMor-1 produced the highest levels of virus throughout infection, suggesting thymic adaptation of this strain. Similar to other virulent strains, Ed-wt, Hu2, and pMor-1 caused a decrease in the number of viable thymocytes as assessed by trypan blue exclusion and fluorescence-activated cell sorter analysis. Thymic architecture was also disrupted by these strains. Sequence analysis of the hemagglutinin (H) and matrix (M) genes showed no common changes in Hu2 and pMor-1. M sequences were identical in pMor-1 and Mor and varied in H at amino acid 469 (threonine to alanine), a position near the base of propeller 4 in the propeller blade/stem model of H structure. Further study will provide insights into the determinants of virulence.

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3

Bellam Muralidhar, Nanda Kishore, Natalie Rauter, Andrey Mikhaylenko, Rolf Lammering, and Dirk A. Lorenz. "Parametric Model Order Reduction of Guided Ultrasonic Wave Propagation in Fiber Metal Laminates with Damage." Modelling 2, no. 4 (November 3, 2021): 591–608. http://dx.doi.org/10.3390/modelling2040031.

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This paper focuses on parametric model order reduction (PMOR) of guided ultrasonic wave propagation and its interaction with damage in a fiber metal laminate (FML). Structural health monitoring in FML seeks to detect, localize and characterize the damage with high accuracy and minimal use of sensors. This can be achieved by the inverse problem analysis approach, which employs the signal measurement data recorded by the embedded sensors in the structure. The inverse analysis requires us to solve the forward simulation of the underlying system several thousand times. These simulations are often exorbitantly expensive and trigger the need for improving their computational efficiency. A PMOR approach hinged on the proper orthogonal decomposition method is presented in this paper. An adaptive parameter sampling technique is established with the aid of a surrogate model to efficiently update the reduced-order basis in a greedy fashion. A numerical experiment is conducted to illustrate the parametric training of the reduced-order model. The results show that the reduced-order solution based on the PMOR approach is accurately complying with that of the high fidelity solution.

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Roy, Ananya, M. Nabi, and Najmur Rahman. "Finite element compatible matrix interpolation for parametric model order reduction of electrothermal microgripper." Journal of Computational Design and Engineering 8, no. 6 (December 2021): 1622–35. http://dx.doi.org/10.1093/jcde/qwab066.

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Abstract Electrothermal microgrippers are nowadays commonly used as they are small in size, low cost, and easy to manufacture. The microgripper is modeled through partial differential equations, which are discretized by the finite element method (FEM), producing a large number of ordinary differential equations. This makes model order reduction (MOR) a fruitful proposition. Moreover, in design applications, the microgripper needs to be simulated repeatedly, with varying values of certain parameters. When these parameters are geometric in nature, matrix interpolation-based parametric model order reduction (pMOR) is the most suited method. However, in finite element applications, the sizes of the FE matrices change for varying geometrical parameters. The conventional matrix interpolation-based pMOR method becomes inapplicable for such cases, which remains a major drawback of this otherwise powerful algorithm. In this paper, this major hurdle is removed, and a new finite element compatible matrix interpolation (FEMI) for pMOR is proposed. A three-dimensional electrothermal microgripper is modeled parametrically using this proposed methodology. The effectiveness of the proposed method is shown through simulation results.

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5

Morre, D. M., G. Lenaz, and D. J. Morre. "Surface oxidase and oxidative stress propagation in aging." Journal of Experimental Biology 203, no. 10 (May 15, 2000): 1513–21. http://dx.doi.org/10.1242/jeb.203.10.1513.

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This report summarizes new evidence for a plasma-membrane-associated hydroquinone oxidase designated as CNOX (constitutive plasma membrane NADH oxidase) that functions as a terminal oxidase for a plasma membrane oxidoreductase (PMOR) electron transport chain to link the accumulation of lesions in mitochondrial DNA to cell-surface accumulations of reactive oxygen species. Previous considerations of plasma membrane redox changes during aging have lacked evidence for a specific terminal oxidase to catalyze a flow of electrons from cytosolic NADH to molecular oxygen (or to protein disulfides). Cells with functionally deficient mitochondria become characterized by an anaerobic metabolism. As a result, NADH accumulates from the glycolytic production of ATP. Elevated PMOR activity has been shown to be necessary to maintain the NAD(+)/NADH homeostasis essential for survival. Our findings demonstrate that the hyperactivity of the PMOR system results in an NADH oxidase (NOX) activity capable of generating reactive oxygen species at the cell surface. This would serve to propagate the aging cascade both to adjacent cells and to circulating blood components. The generation of superoxide by NOX forms associated with aging is inhibited by coenzyme Q and provides a rational basis for the anti-aging activity of circulating coenzyme Q.

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6

Setiawan, Fery, Arif Rahman Nurdianto, Rizal Fauzi Nurdianto, Heribertus Agustinus B. Tena, and Jenny Sunariani. "PERAN KAPSAISIN PADA PENGHAMBATAN TERJADINYA KANKER." MEDFARM: Jurnal Farmasi dan Kesehatan 10, no. 2 (December 30, 2021): 51–64. http://dx.doi.org/10.48191/medfarm.v10i2.62.

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Kapsaisin (8-methyl-N-vanillyl-6-nonanenamide) adalah rasa pedas utama dalam batang cabai yang dikonsumsi secara umum. Fungsi utama kapsaisin adalah mengurangi rasa nyeri dan inflamasi neurogenik, selain itu juga memiliki efek anti-tumor. Reseptor kapsaisin dan substansi lain yang terkait dengan kapsaisin telah diidentifikasi dan dikloningkan. Reseptor ini adalah reseptor vaniloid subtipe 1 (VR-1), yang membentuk saluran kation non-selektif dalam membran plasma yang memediasi beberapa efek pleitropik. Kapsaisin dan substansi analog yang sejenis mempunyai efek anti-proliferatif dalam sel kanker manusia melalui induksi siklus sel fase G0/G1 sehingga proliferasi sel terhenti dan terjadi apoptosis pada beberapa sel yang mengalami transformasi. Kemampuan untuk menginduksi apoptosis merupakan aktivitas supresi plasma membran NADH-oksidoreduktase (PMOR), enzim yang mentransfer elektron dari sitoplasmik NADH melalui ko-enzim Q (ubikuinon) ke akseptor elektron eksternal, seperti oksigen. Selain menginduksi apoptosis melalui PMOR, kapsaisin juga menginduksi peningkatan p21, Bax, dan Poly ADP Ribose Polymerase (PARP). Mekanisme antitumor kapsaisin secara utama dengan melakukan stabilisasi dan aktivasi gen p53. Kapsaisin secara substansial memperpanjang siklus hidup p53 dan secara signifikan meningkatkan aktivitas transkripsional p53. PMOR diperkirakan terlibat dalam pengaturan pertumbuhan sel dan proliferasi dengan menjaga rasio NADH+/NADH yang sesuai dengan kebutuhan sel.

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Yamaki, M., G. R. O. Menezes, A. L. C. Paiva, L. Barbosa, R. F. Silva, R. B. Teixeira, R. A. Torres, and P. S. Lopes. "Estudo de características de produção de matrizes de corte por meio da análise de componentes principais." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 61, no. 1 (February 2009): 227–31. http://dx.doi.org/10.1590/s0102-09352009000100032.

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Avaliou-se o descarte de variáveis de produção, em análises de componentes principais, de três linhagens de matrizes de corte do Programa de Melhoramento Genético da Universidade Federal de Viçosa, utilizando informações de 270 aves, sendo 90 de cada linhagem. As características analisadas foram dias para postura do primeiro ovo (DPPO), taxa de postura da 22ª a 56ª semana (TP), peso médio individual na 32ª (PMI1), na 40ª (PMI2), na 48ª (PMI3), na 56ª (PMI4) e na 64ª semana (PMI5) e peso médio do ovo, obtido pela média da pesagem de três ovos na 32ª (PMO1), na 40ª (PMO2), na 48ª (PMO3), na 56ª (PMO4) e na 64ª semana (PMO5). Dos 12 componentes principais, sete apresentaram variância menor do que 0,7 (autovalor menor do que 0,7), sugerindo-se sete variáveis para descarte. As variáveis descartadas foram aquelas que apresentaram maiores coeficientes, em valor absoluto, a partir do último componente principal. Observou-se correlação linear simples e significativa entre as variáveis descartadas e as não descartadas, que indica redundância de variáveis, razão do descarte. Recomendam-se as variáveis: DPPO, TP, PM14, PMO1 e PMO4 para o estudo de características da produção de matrizes de frango de corte por meio da análise de componentes principais.

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Kormann, A. C. M., K. F. Portella, P. N. Pereira, and R. P. Santos. "Study of the performance of four repairing material systems for hydraulic structures of concrete dams." Cerâmica 49, no. 309 (March 2003): 48–54. http://dx.doi.org/10.1590/s0366-69132003000100011.

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Four types of repairing materials are studied as function of either a conventional concrete or a reference-concrete (RefC), these are: polymer-modified cement mortar (PMor), steel fiber concrete (SFco), epoxy mortar (EMor) and silica fume mortar (SFmo), to be applied in hydraulic structures surfaces subjected to a high velocity water flow. Besides the mechanical requests and wearing resistance of hydraulic concrete dam structures, especially the spillway surfaces, the high solar radiation, the environmental temperature and wet and dry cycles, contribute significantly to the reduction of their lifespan. RefC and the SFco were developed based on a usual concrete mixture used in slabs of spillways. The average RefC mixture used was 1: 1.61: 2.99: 0.376, with Pozzolan-modified Portland cement consumption of 425 kg/m³. EMor and PMor mixtures followed the information given by the manufacturers and lab experience. Tests on concrete samples were carried out in laboratory simulating normally found environmental situations in order to control the mechanical resistance and the aging imposed conditions, such as solar radiation and humidity. Also, physicochemical characterizing tests were made for all used materials. From the analyzed results, two of them presented a higher performance: the EMor and SFmo. SFco presented good adherence to the RefC and good mechanical performance. However, it also presented apparent metal corrosion in humidity tests, being indicated for use, with caution, as an intermediate layer in underwater repairs. In a general classification, considering all tests, including their field applications, the better performance material systems were EMor- SFmo> SFco> PMor.

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Okita, Noriko, Toshihiro Hoaki, Shinya Suzuki, and Masashi Hatamoto. "Characteristics of aerobic methane-oxidising bacterial community at the sea-floor surface of the Nankai Trough." Marine and Freshwater Research 71, no. 10 (2020): 1252. http://dx.doi.org/10.1071/mf19317.

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Methane hydrate is one of the new energy sources, but methane leakage could cause environmental issues such as marine ecosystem changes and global warming. The methane-oxidising bacterial (MOB) community could reflect the methane concentration, thus it may be an indicator of methane leakage. We obtained two sea-floor surface samples from a methane seep area and 12 samples from other general sea-floor areas of the Nankai Trough for the detection and phylogenetic analysis of the particulate methane monooxygenase (pmoA) gene. Using quantitative polymerase chain reaction analysis, the methane seep samples were found to have 106 copies of the pmoA gene per gram of sediment, whereas the general sea-floor area samples of the Nankai Trough contained 103–104 copies of the gene per gram of sediment. Phylogenetic analysis of the pmoA gene sequences showed that the sequences detected in the general and methane seep area samples differed significantly. Specifically, the pmof1–pmor primer pair could detect pmoA genes for the methane seep area, whereas pmoA gene from the general seafloor samples could be detected only using the A189–mb661 primer pair. The results of this study may facilitate the detection of unintended leakage of methane at methane hydrate production sites by monitoring MOB communities using pmoA-targeted phylogenetic analysis and quantification.

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10

Yamaki, Marcos, Gilberto Romeiro de Oliveira Menezes, Rafael Bastos Teixeira, Leandro Barbosa, André Luis da Costa Paiva, and Robledo de Almeida Torres. "Divergência genética entre linhagens de matrizes de corte por meio de análise de agrupamento." Revista Brasileira de Zootecnia 37, no. 5 (May 2008): 829–33. http://dx.doi.org/10.1590/s1516-35982008000500008.

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Este trabalho foi realizado com o objetivo de avaliar a divergência genética de três linhagens de matrizes de corte do Programa de Melhoramento Genético da UFV. Foram avaliados dados de 270 aves, 90 de cada linhagem, para estudo das características dias para o primeiro ovo (DPPO), taxa de postura da 22ª à 56ª semana (TP), peso médio individual na 32ª (PMI1), 40ª (PMI2), 48ª (PMI3), 56ª (PMI4) e 64ª semanas de idade (PMI5); e peso médio do ovo, obtido pela média da pesagem de três ovos na 32ª (PMO1), 40ª (PMO2), 48ª (PMO3), 56ª (PMO4) e 64ª semanas de idade (PMO5). Para avaliar a divergência, foram utilizados dois métodos: hierárquico do vizinho mais próximo e otimização de Tocher. Pelo método hierárquico do vizinho mais próximo, utilizando-se a distância de Mahalanobis ao quadrado (D²) como medida de dissimilaridade, formou-se um único grupo. Pelo método de otimização de Tocher, foram formados dois grupos, comprovando que os dois métodos foram discordantes na avaliação da divergência genética de linhas de aves de corte. As características que apresentaram as contribuições relativas mais expressivas para a divergência foram, respectivamente, PMO1 (25,71%), DDPO (21,76%), PMI4 (17,65%) e PMI2 (13,04%).

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Paiva, André Luis da Costa, Rafael Bastos Teixeira, Marcos Yamaki, Gilberto Romeiro de Oliveira Menezes, Carla Daniela Suguimoto Leite, and Robledo de Almeida Torres. "Análise de componentes principais em características de produção de aves de postura." Revista Brasileira de Zootecnia 39, no. 2 (February 2010): 285–88. http://dx.doi.org/10.1590/s1516-35982010000200009.

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Para avaliar a possibilidade de descarte de variáveis de produção em 942 aves de postura por meio de componentes principais, visando eliminar características redundantes e de difícil mensuração, foram utilizados os dados obtidos de linhagens de aves de postura do Programa de Melhoramento Genético da Universidade Federal de Viçosa. As características analisadas foram: taxa de postura da 26ª à 58ª semana de idade (TP); peso médio individual na 34ª (PMI1), na 42ª (PMI2), na 50ª (PMI3), na 58ª (PMI4) e na 66ª semana (PMI5); e peso médio do ovo na 34ª (PMO1), na 42ª (PMO2), na 50ª (PMO3), na 58ª (PMO4) e na 66ª semana de idade (PMO5). Dos 11 componentes principais, oito apresentaram variância inferior a 0,7 (autovalor inferior a 0,7), o que sugere oito variáveis para descarte. As variáveis descartadas foram aquelas com maiores coeficientes, em valor absoluto, a partir do último componente principal, uma vez que variáveis altamente correlacionadas aos componentes principais de menor variância representam variação praticamente insignificante. As variáveis descartadas apresentaram correlação linear simples significativa com as demais, ou seja, foram redundantes. Com base nesses resultados, recomendam-se as seguintes variáveis para utilização em experimentos futuros: TP, PMI1 e PMO4.

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12

Kulik, Kamila, Barbara Żyżyńska-Granica, Agnieszka Kowalczyk, Przemysław Kurowski, Małgorzata Gajewska, and Magdalena Bujalska-Zadrożny. "Magnesium and Morphine in the Treatment of Chronic Neuropathic Pain–A Biomedical Mechanism of Action." International Journal of Molecular Sciences 22, no. 24 (December 18, 2021): 13599. http://dx.doi.org/10.3390/ijms222413599.

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The effectiveness of opioids in the treatment of neuropathic pain is limited. It was demonstrated that magnesium ions (Mg2+), physiological antagonists of N-methyl-D-aspartate receptor (NMDAR), increase opioid analgesia in chronic pain. Our study aimed to determine the molecular mechanism of this action. Early data indicate the cross-regulation of µ opioid receptor (MOR) and NMDAR in pain control. Morphine acting on MOR stimulates protein kinase C (PKC), while induction of NMDAR recruits protein kinase A (PKA), leading to a disruption of the MOR-NMDAR complex and promoting functional changes in receptors. The mechanical Randall-Selitto test was used to assess the effect of chronic Mg2+ and morphine cotreatment on streptozotocin-induced hyperalgesia in Wistar rats. The level of phosphorylated NMDAR NR1 subunit (pNR1) and phosphorylated MOR (pMOR) in the periaqueductal gray matter was determined with the Western blot method. The activity of PKA and PKC was examined by standard enzyme immunoassays. The experiments showed a reduction in hyperalgesia after coadministration of morphine (5 mg/kg intraperitoneally) and Mg2+ (40 mg/kg intraperitoneally). Mg2+ administered alone significantly decreased the level of pNR1, pMOR, and activity of both tested kinases. The results suggest that blocking NMDAR signaling by Mg2+ restores the MOR-NMDAR complex and thus enables morphine analgesia in neuropathic rats.

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Ahmad, Mian Ilyas, Peter Benner, and Lihong Feng. "Interpolatory model reduction for quadratic-bilinear systems using error estimators." Engineering Computations 36, no. 1 (December 27, 2018): 25–44. http://dx.doi.org/10.1108/ec-04-2018-0162.

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Purpose The purpose of this paper is to propose an interpolation-based projection framework for model reduction of quadratic-bilinear systems. The approach constructs projection matrices from the bilinear part of the original quadratic-bilinear descriptor system and uses these matrices to project the original system. Design/methodology/approach The projection matrices are constructed by viewing the bilinear system as a linear parametric system, where the input associated with the bilinear part is treated as a parameter. The advantage of this approach is that the projection matrices can be constructed reliably by using an a posteriori error bound for linear parametric systems. The use of the error bound allows us to select a good choice of interpolation points and parameter samples for the construction of the projection matrices by using a greedy-type framework. Findings The results are compared with the standard quadratic-bilinear projection methods and it is observed that the approximations through the proposed method are comparable to the standard method but at a lower computational cost (offline time). Originality/value In addition to the proposed model order reduction framework, the authors extend the one-sided moment matching parametric model order reduction (PMOR) method to a two-sided method that doubles the number of moments matched in the PMOR method.

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Zalavadia, Hardikkumar, and Eduardo Gildin. "Two-Step Predict and Correct Non-Intrusive Parametric Model Order Reduction for Changing Well Locations Using a Machine Learning Framework." Energies 14, no. 6 (March 22, 2021): 1765. http://dx.doi.org/10.3390/en14061765.

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The objective of this paper is to develop a two-step predict and correct non-intrusive Parametric Model Order Reduction (PMOR) methodology for the problem of changing well locations in an oil field that can eventually be used for well placement optimization to gain significant computational savings. In this work, we propose a two-step PMOR procedure, where, in the first step, a Proper Orthogonal Decomposition (POD)-based strategy that is non-intrusive to the simulator source code is introduced, as opposed to the convention of using POD as a simulator intrusive procedure. The non-intrusiveness of the proposed technique stems from formulating a novel Machine Learning (ML)-based framework used with POD. The features of the ML model (Random Forest was used here) are designed such that they take into consideration the temporal evolution of the state solutions and thereby avoid simulator access for the time dependency of the solutions. The proposed PMOR method is global, since a single reduced-order model can be used for all the well locations of interest in the reservoir. We address the major challenge of the explicit representation of the well location change as a parameter by introducing geometry-based features and flow diagnostics-inspired physics-based features. In the second step, an error correction model based on reduced model solutions is formulated to correct for discrepancies in the state solutions at well grid blocks expected from POD basis for new well locations. The error correction model proposed uses Artificial Neural Networks (ANNs) that consider the physics-based reduced model solutions as features, and is proved to reduce the error in QoI (Quantities of Interest), such as oil production rates and water cut, significantly. This workflow is applied to a simple homogeneous reservoir and a heterogeneous channelized reservoir using a section of SPE10 model that showed promising results in terms of model accuracy. Speed-ups of about 50×–100× were observed for different cases considered when running the test scenarios. The proposed workflow for Reduced-Order Modeling is “non-intrusive” and hence can increase its applicability to any simulator used. Additionally, the method is formulated such that all the simulation time steps are independent and hence can make use of parallel resources very efficiently and also avoid stability issues that can result from error accumulation over time steps.

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W. Hess, Martin, and Peter Benner. "A reduced basis method for microwave semiconductor devices with geometric variations." COMPEL: The International Journal for Computation and Mathematics in Electrical and Electronic Engineering 33, no. 4 (July 1, 2014): 1071–81. http://dx.doi.org/10.1108/compel-12-2012-0377.

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Purpose – The Reduced Basis Method (RBM) generates low-order models of parametrized PDEs to allow for efficient evaluation of parametrized models in many-query and real-time contexts. The purpose of this paper is to investigate the performance of the RBM in microwave semiconductor devices, governed by Maxwell's equations. Design/methodology/approach – The paper shows the theoretical framework in which the RBM is applied to Maxwell's equations and present numerical results for model reduction under geometry variation. Findings – The RBM reduces model order by a factor of $1,000 and more with guaranteed error bounds. Originality/value – Exponential convergence speed can be observed by numerical experiments, which makes the RBM a suitable method for parametric model reduction (PMOR).

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Gilbert, Bettina, Ian R. McDonald, Ruth Finch, Graham P. Stafford, Allan K. Nielsen, and J. Colin Murrell. "Molecular Analysis of the pmo (Particulate Methane Monooxygenase) Operons from Two Type II Methanotrophs." Applied and Environmental Microbiology 66, no. 3 (March 1, 2000): 966–75. http://dx.doi.org/10.1128/aem.66.3.966-975.2000.

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ABSTRACT The particulate methane monooxygenase gene clusters,pmoCAB, from two representative type II methanotrophs of the α-Proteobacteria, Methylosinus trichosporium OB3b and Methylocystis sp. strain M, have been cloned and sequenced. Primer extension experiments revealed that the pmo cluster is probably transcribed from a single transcriptional start site located 300 bp upstream of the start of the first gene, pmoC, for Methylocystis sp. strain M. Immediately upstream of the putative start site, consensus sequences for ς70 promoters were identified, suggesting that thesepmo genes are recognized by ς70 and negatively regulated under low-copper conditions. The pmogenes were cloned in several overlapping fragments, since parts of these genes appeared to be toxic to the Escherichia colihost. Methanotrophs contain two virtually identical copies ofpmo genes, and it was necessary to use Southern blotting and probing with pmo gene fragments in order to differentiate between the two pmoCAB clusters in both methanotrophs. The complete DNA sequence of one copy of pmogenes from each organism is reported here. The gene sequences are 84% similar to each other and 75% similar to that of a type I methanotroph of the γ-Proteobacteria, Methylococcus capsulatus Bath. The derived proteins PmoC and PmoA are predicted to be highly hydrophobic and consist mainly of transmembrane-spanning regions, whereas PmoB has only two putative transmembrane-spanning helices. Hybridization experiments showed that there are two copies ofpmoC in both M. trichosporium OB3b andMethylocystis sp. strain M, and not three copies as found in M. capsulatus Bath.

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Nasisi, Salvatore, Michael Valášek, and Tomáš Vampola. "AN INTERPOLATION METHOD FOR DETERMINING THE FREQUENCIES OF PARAMETERIZED LARGE-SCALE STRUCTURES." Acta Polytechnica 55, no. 6 (December 31, 2015): 407. http://dx.doi.org/10.14311/ap.2015.55.0407.

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<p>Parametric Model Order Reduction (pMOR) is an emerging category of models developed with the aim of describing reduced first and second-order dynamical systems. The use of a pROM turns out useful in a variety of applications spanning from the analysis of Micro-Electro-Mechanical Systems (MEMS) to the optimization of complex mechanical systems because they allow predicting the dynamical behavior at any values of the quantities of interest within the design space, e.g. material properties, geometric features or loading conditions. The process underlying the construction of a pROM using an SVD-based method [18] accounts for three basic phases: a) construction of several local ROMs (Reduced Order Models); b) projection of the state-space vector onto a common subspace spanned by several transformation matrices derived in the first step; c) use of an interpolation method capable of capturing for one or more parameters the values of the quantity of interest. One of the major difficulties encountered in this process has been identified at the level of the interpolation method and can be encapsulated in the following contradiction: if the number of detailed finite element analyses is high then an interpolation method can better describe the system for a given choice of a parameter but the time of computation is higher. In this paper is proposed a method for removing the above contradiction by introducing a new interpolation method (RSDM). This method allows to restore and make available to the interpolation tool certain <em>natural</em> components belonging to the matrices of the full FE model that are related on one side, to the process of reduction and on the other side, to the characteristics of a solid in the FE theory. This approach shows higher accuracy than methods used for the assessment of the system’s eigenbehavior. To confirm the usefulness of the RSDM a Hexapod will be analyzed.</p>

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MINAGUCHI, K., and K. SUZUKI. "Genetic polymorphisms of the Pmol and Pmo2 salivary proteins detected by the modified protein staining method." Annals of Human Genetics 52, no. 3 (July 1988): 177–87. http://dx.doi.org/10.1111/j.1469-1809.1988.tb01095.x.

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Esquivel, Dolores, Els De Canck, César Jiménez-Sanchidrián, Francisco J. Romero-Salguero, and Pascal Van Der Voort. "Pyrrole PMOs, incorporating new N-heterocyclic compounds on an ethene-PMO through Diels–Alder reactions." Materials Chemistry and Physics 148, no. 1-2 (November 2014): 403–10. http://dx.doi.org/10.1016/j.matchemphys.2014.08.004.

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Koo, Christopher W., Frank J. Tucci, Yuan He, and Amy C. Rosenzweig. "Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer." Science 375, no. 6586 (March 18, 2022): 1287–91. http://dx.doi.org/10.1126/science.abm3282.

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Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using inactive, detergent-solubilized pMMO lack several conserved regions neighboring the proposed active site. We show that reconstituting pMMO in nanodiscs with lipids extracted from the native organism restores methane oxidation activity. Multiple nanodisc-embedded pMMO structures determined by cryo–electron microscopy to 2.14- to 2.46-angstrom resolution reveal the structure of pMMO in a lipid environment. The resulting model includes stabilizing lipids, regions of the PmoA and PmoC subunits not observed in prior structures, and a previously undetected copper-binding site in the PmoC subunit with an adjacent hydrophobic cavity. These structures provide a revised framework for understanding and engineering pMMO function.

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Guibourdenche, Jean, Michèle Noël, Didier Chevenne, Edith Vuillard, Jean Luc Voluménie, Michel Polak, Christine Boissinot, Dominique Porquet, and Dominique Luton. "Biochemical Investigation of Foetal and Neonatal Thyroid Function Using the ACS-180SE Analyser: Clinical Application." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 38, no. 5 (September 2001): 520–26. http://dx.doi.org/10.1177/000456320103800509.

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Despite sonographic detection of foetal goitre, uncertainty persists in the initial diagnosis of thyrotoxicosis and hypothyroidism. The aim of this study was to establish foetal and neonatal iodothyronine and thyrotrophin reference values for the ACS-180SE analyser. From 22 to 36 weeks of gestation, median foetal serum free thyroxine (FT4) levels increased from 6·0 pmol/L to 14·3 pmol/L, while free triiodothyronine (FT3) levels increased from 0·7 pmoI/L to 1·9 pmol/L and mean thyrotrophin (TSH) levels remained stable (10·2±3·8 mU/L; n=33). At birth, concentrations were independent of gender and gestational age. Among the 10 cases of sonographically detected foetal goitre, serum TSH and FT4 were measured in five, showing hypothyroidism (3/5) or hyperthyroidism (2/5). Cord blood TSH levels reflected the efficacy of prenatal therapy. Measurement of foetal FT4 and TSH can be used to confirm foetal thyroid dysfunction, whereas treatment efficacy can be assessed sonographically and confirmed by measurement of TSH assay at birth.

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Yoon, Tae-Ho, Sung-Wook Choi, Soo-Yeon Kim, and Daecheol Kim. "Differences in the Perceptions of PMs and PMOs on the Importance of the Role of PMO." Journal of Society of Korea Industrial and Systems Engineering 44, no. 3 (September 30, 2021): 207–13. http://dx.doi.org/10.11627/jkise.2021.44.3.207.

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Sun, Yan, Minxiao Wang, Leilei Li, Li Zhou, Xiaocheng Wang, Ping Zheng, Haiyan Yu, Chaolun Li, and Song Sun. "Molecular identification of methane monooxygenase and quantitative analysis of methanotrophic endosymbionts under laboratory maintenance inBathymodiolus platifronsfrom the South China Sea." PeerJ 5 (August 7, 2017): e3565. http://dx.doi.org/10.7717/peerj.3565.

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Deep-sea mussels of the genusBathymodiolusare numerically dominant macrofauna in many cold seep and hydrothermal vent ecosystems worldwide, and they depend on organic carbon produced by symbionts present in the epithelial cells of the gills. AlthoughBathymodiolus platifronsrepresents typical methanotrophic endosymbiosis, our understanding of molecular mechanisms of methane oxidization and carbon fixation is still in its infancy. Moreover, the laboratory maintenance ofB. platifronsand the symbiont abundance dynamics during maintenance has not been reported. In the present study, we report the first systematic identification and phylogenetic analysis of three subunits of methane monooxygenase (pmoA, pmoB, and pmoC) obtained from the endosymbiotic bacteria found inB. platifrons. The coding sequences (CDS) of the three genes in theB. platifronsendosymbiont were 750, 1,245, and 753 bp, encoding 249, 414, and 250 amino acids, respectively. Sequence alignment and phylogenetic analysis revealed that the symbiont ofB. platifronsbelongs to the type I methanotrophs. In order to clarify the impact of environmental methane on symbiont abundance, a 34-day laboratory maintenance experiment was conducted in whichB. platifronsindividuals were acclimatized to methane-present and methane-absent environments. Symbiont abundance was evaluated by calculating the relative DNA content of the methane monooxygenase gene using quantitative real-time PCR. We found that symbiont quantity immediately decreased from its initial level, then continued to gradually decline during maintenance. At 24 and 34 days of maintenance, symbiont abundance in the methane-absent environment had significantly decreased compared to that in the methane-present environment, indicating that the maintenance of symbionts relies on a continuous supply of methane. Our electron microscopy results validated the qPCR analysis. This study enriches our knowledge of the molecular basis and the dynamic changes of the methanotrophic endosymbiosis inB. platifrons, and provides a feasible model biosystem for further investigation of methane oxidization, the carbon fixation process, and environmental adaptations of deep-sea mussels.

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Ross, Matthew O., Fraser MacMillan, Jingzhou Wang, Alex Nisthal, Thomas J. Lawton, Barry D. Olafson, Stephen L. Mayo, Amy C. Rosenzweig, and Brian M. Hoffman. "Particulate methane monooxygenase contains only mononuclear copper centers." Science 364, no. 6440 (May 9, 2019): 566–70. http://dx.doi.org/10.1126/science.aav2572.

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Bacteria that oxidize methane to methanol are central to mitigating emissions of methane, a potent greenhouse gas. The nature of the copper active site in the primary metabolic enzyme of these bacteria, particulate methane monooxygenase (pMMO), has been controversial owing to seemingly contradictory biochemical, spectroscopic, and crystallographic results. We present biochemical and electron paramagnetic resonance spectroscopic characterization most consistent with two monocopper sites within pMMO: one in the soluble PmoB subunit at the previously assigned active site (CuB) and one ~2 nanometers away in the membrane-bound PmoC subunit (CuC). On the basis of these results, we propose that a monocopper site is able to catalyze methane oxidation in pMMO.

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Agosti, Maristella, Ernesto William De Luca, Séamus Lawless, and Johannes Leveling. "PMHR 2011." ACM SIGIR Forum 45, no. 2 (January 9, 2012): 94–98. http://dx.doi.org/10.1145/2093346.2093362.

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Tshuma, Brighton, Herman Steyn, and Cornelis Cristo van Waveren. "The mediation role of the PMO in the transfer of knowledge between projects – a case study of five PMOs." International Journal of Managing Projects in Business 15, no. 1 (November 16, 2021): 150–74. http://dx.doi.org/10.1108/ijmpb-03-2021-0063.

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PurposeThis article describes advances in the study of knowledge transfer (KT) in project-based organizations (PBOs). Project management offices (PMOs) have both a moderation role and a mediation role to play in KT between projects. In order to improve KT between projects, this paper explores the mediation role of the PMO in the transfer of knowledge with different levels of articulability. The aim is to improve the usability of transferred knowledge.Design/methodology/approachThe case study method was used to investigate KT in five-divisional PMOs within a multinational engineering and project management PBO. Fifteen semi-structured interviews were conducted and the results were analysed using ATLAS.Ti (a computer-aided qualitative data analysis software).FindingsThe findings show that it is the way in which the PMO structures knowledge management (KM) infrastructure and processes, which determines the success of its mediation role in the transfer of tacit and explicit knowledge between projects. The articulability of knowledge influences the PMO's mediation role and the PMO's mediation role in turn improves the usability of knowledge, thereby creating a conducive environment for a competitive advantage.Originality/valueThis study offers a framework to assist scholars and practitioners to understand the mediation role of the PMO in the transfer of knowledge with different levels of articulability within the projects environment. Such understanding can improve the usability of transferred knowledge, thereby creating a competitive edge for a PBO. The study shows that the PMO can be used as an instrument for KT between projects, a theme that was not found in literature. The paper thus offers new empirical information.

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Garcia-Lacarte, Marcos, Maria L. Mansego, M. Angeles Zulet, J. Alfredo Martinez, and Fermin I. Milagro. "miR-1185-1 and miR-548q Are Biomarkers of Response to Weight Loss and Regulate the Expression of GSK3B." Cells 8, no. 12 (November 30, 2019): 1548. http://dx.doi.org/10.3390/cells8121548.

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The aim of the present investigation was to identify putative miRNAs involved in the response to weight loss. Reverse-transcribed RNA isolated from white blood cells (WBCs) of a subpopulation from the Reduction of the Metabolic Syndrome in Navarra-Spain (RESMENA-S) study (low-responders (LR) and high-responders (HR)) was hybridized in a gene expression microarray. Moreover, miRNAs were sequenced by miRNA-Seq. It was found that miR-548q and miR-1185-1 were overexpressed in HR, both in the microarray and in the miRNA-Seq. A bioinformatic prediction of putative target genes of the selected miRNAs found that GSK3B, a putative target for miR-548q and miR-1185-1, was downregulated in HR. Particular 3′-UTR binding regions of GSK3B were cloned downstream of the firefly luciferase gene. HEK-293T cells were co-transfected with either 0.25 μg of empty pmiR-GLO or pmiR-GLO-548q-3′-UTR/pmiR-GLO-1185-1-3′-UTR, and 7.5 pmol of miR-548q/miR-1185-1 mimics, demonstrating that miR-1185-1 bound to the 3′-UTR region of GSK3B. THP-1 cells were transfected with either 20/40 nM of miR-548q/miR-1185-1 mimics, evidencing that miR-1185-1inhibited the expression of the gene when transfected at doses of 20/40 nM, whereas miR-548q inhibited GSK3B expression at a dose of 40 nM. As a conclusion, miR-548q and miR-1185-1 levels in WBCs are biomarkers of response to weight-loss diets and could be involved in the regulation of the proinflammatory gene GSK3B.

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Seo, Yong Won, and Duck Hee Lee. "A Study on the Development of PMO Cost Estimation Model." Journal of the Korea society of IT services 13, no. 4 (December 31, 2014): 169–88. http://dx.doi.org/10.9716/kits.2014.13.4.169.

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Jung, KiWook, SangMin Woo, HyunJoon Jung, and SoYoung Kim. "V-shaped Nanosheet FET for PMOS Performance Enhancement." Journal of the Institute of Electronics and Information Engineers 59, no. 10 (October 31, 2022): 57–63. http://dx.doi.org/10.5573/ieie.2022.59.10.57.

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30

Kousik, Chandrasekar S., Jennifer Ikerd, Mihir Mandal, Scott Adkins, and William W. Turechek. "Watermelon Germplasm Lines USVL608-PMR, USVL255-PMR, USVL313-PMR, and USVL585-PMR with Broad Resistance to Powdery Mildew." HortScience 53, no. 8 (August 2018): 1212–17. http://dx.doi.org/10.21273/hortsci12979-18.

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Hsu, B. C., C. W. Liu, W. T. Liu, and C. H. Lin. "A PMOS tunneling photodetector." IEEE Transactions on Electron Devices 48, no. 8 (2001): 1747–49. http://dx.doi.org/10.1109/16.936700.

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Kim, Ki-Hyun, Geun-Wan Park, and Seung-June Hwang. "An Evaluation Model of IT PMO Performance Using Pentagon Model." Journal of Society for e-Business Studies 21, no. 4 (November 30, 2016): 119–36. http://dx.doi.org/10.7838/jsebs.2016.21.4.119.

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Louch, Gemma, Caroline Reynolds, Sally Moore, Claire Marsh, Jane Heyhoe, Abigail Albutt, and Rebecca Lawton. "Validation of revised patient measures of safety: PMOS-30 and PMOS-10." BMJ Open 9, no. 11 (November 2019): e031355. http://dx.doi.org/10.1136/bmjopen-2019-031355.

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ObjectivesThere is growing evidence that patients can provide feedback on the safety of their care. The 44-item Patient Measure of Safety (PMOS) was developed for this purpose. While valid and reliable, the length of this questionnaire makes it potentially challenging for routine use. Our study aimed to produce revised, shortened versions of PMOS (PMOS-30 and PMOS-10), which retained the psychometric properties of the longer version.ParticipantsTo produce a shortened diagnostic measure, we analysed data from 2002 patients who completed PMOS-44, and examined the reliability of the revised measure (PMOS-30) in a sample of 751 patients. To produce a brief standalone measure, we again analysed data from 2002 patients who completed PMOS-44, and tested the reliability and validity of the brief standalone measure (PMOS-10) in a sample of 165 patients.MethodsThe process of shortening the questionnaire involved a combination of secondary data analysis (eg, Standard Deviation and inter-item correlations) and a consensus group exercise to produce PMOS-30 and examine face validity. Analysis of PMOS-30 data examined reliability (eg, Cronbach’s alpha). Further secondary data analysis (ie, corrected item-total correlations) produced PMOS-10, and primary data collection assessed its reliability and validity (eg, Cronbach’s alpha, analysis of variance).ResultsFourteen items were removed to produce PMOS-30 and the percentage of negatively worded items was reduced from 57% to 33%. PMOS-30 demonstrated good internal reliability (α=0.89). The 10 items with the highest corrected item-total correlations across both PMOS-44 and PMOS-30 composed PMOS-10. PMOS-10 had good internal reliability (α=0.79), demonstrated convergent validity; however, discriminant validity was not established.ConclusionsTwo revised, shortened versions of the original PMOS-44 (PMOS-30 and PMOS-10) were produced to capture patient feedback about safety in hospital. The measures demonstrated good reliability and validity, and preserved the psychometric properties of the original measure.

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van der Vliet, Jacques. "Praktijkvoorbeeld PMO." TBV – Tijdschrift voor Bedrijfs- en Verzekeringsgeneeskunde 24, no. 8 (October 2016): 386–87. http://dx.doi.org/10.1007/s12498-016-0148-z.

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Hulshof, Carel. "Uitvoering PMO." TBV – Tijdschrift voor Bedrijfs- en Verzekeringsgeneeskunde 30, no. 7-8 (October 31, 2022): 15. http://dx.doi.org/10.1007/s12498-022-2018-1.

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Kim, Jong-Ki, and Ok-Soo Yoon. "The Effect of PMO Functions on IT Project Performance." Journal of Information Systems 20, no. 3 (September 30, 2011): 129–59. http://dx.doi.org/10.5859/kais.2011.20.3.129.

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Kim, Ki-Hyun, Geun-Wan Park, and Seung-June Hwang. "A Study on the Effect of PMO Capability and Organization Justice on IT Project Performance." Journal of Society for e-Business Studies 21, no. 3 (August 31, 2016): 129–51. http://dx.doi.org/10.7838/jsebs.2016.21.3.129.

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Cruz, Cristiane Esteves, and Gabriela Scur. "Alinhamento do PMO à Gestão Organizacional: Estudo dos Elementos do PMO sob as Dimensões Estratégica, Tática e Operacional." Revista de Gestão e Projetos 07, no. 01 (April 1, 2016): 32–40. http://dx.doi.org/10.5585/gep.v7i1.380.

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Piekarska, Katarzyna. "PLASMID-MEDIATED QUINOLONE RESISTANCE - PMQR." Postępy Mikrobiologii - Advancements of Microbiology 57, no. 1 (2019): 47–57. http://dx.doi.org/10.21307/pm-2018.57.1.047.

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Savic, Z., S. Stankovic, M. Kovacevic, and M. Petrovic. "Energy Dependence of pMOS Dosemeters." Radiation Protection Dosimetry 64, no. 3 (May 1, 1996): 205–11. http://dx.doi.org/10.1093/oxfordjournals.rpd.a031575.

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Li, G. Y., H. B. Zhao, Y. Xia, F. Zeng, and Y. J. Luo. "PMOE planetary/lunar ephemeris framework." Proceedings of the International Astronomical Union 3, S248 (October 2007): 560–62. http://dx.doi.org/10.1017/s1743921308020140.

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AbstractThe PMOE planetary/lunar ephemeris framework was established in 2003, and has been improved in recent years. In the framework of the post-Newtonian effects, the figure perturbation effects arising from the a finite size of the Sun, Moon and the Earth, and the effect of the Earth tide were taken into account. The accuracy of using the PMOE ephemeris to predict the positions of the planets in the solar system are the same as that of JPL DE 405. Based on this framework, the orbit optimization for the LISA, ASTROD and ASTROD I missions, and the computation of celestial phenomena and lunar phases in the Xia Shang and Zhou period of ancient China have been completed.

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42

Moylan, Peter J. "The PMOS Real-Time Kernel." IFAC Proceedings Volumes 25, no. 11 (June 1992): 125–29. http://dx.doi.org/10.1016/s1474-6670(17)50135-4.

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Ristić, G., S. Golubović, and M. Pejović. "pMOS transistors for dosimetric application." Electronics Letters 29, no. 18 (1993): 1644. http://dx.doi.org/10.1049/el:19931095.

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Gräfenstein, Lydia, and Wolfgang Bensch. "Funktionalisierung von PMOs durch Azobenzoleinheiten." Zeitschrift für anorganische und allgemeine Chemie 638, no. 10 (August 2012): 1615. http://dx.doi.org/10.1002/zaac.201204098.

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Lindert, N., M. Yoshida, C. Wann, and Chenming Hu. "Comparison of GIDL in p/sup +/-poly PMOS and n/sup +/-poly PMOS devices." IEEE Electron Device Letters 17, no. 6 (June 1996): 285–87. http://dx.doi.org/10.1109/55.496459.

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Cha, Hwanju, and Ja-Hee Kim. "An Evaluation Model of Transition PMO Competencies Using RGT and AHP." Journal of the Korea society of IT services 14, no. 2 (June 30, 2015): 87–109. http://dx.doi.org/10.9716/kits.2015.14.2.087.

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McCreight, James D., G. Weston Bohn, and Thomas W. Whitaker. "PMR Honeydew Muskmelon." HortScience 22, no. 1 (February 1987): 177. http://dx.doi.org/10.21273/hortsci.22.1.177.

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Abstract Greenflesh Honeydew (GFHD) musk-melon (Cucumis melo L.) is an erratic performer in the varied environments of Arizona, California (Imperial Valley and San Joaquin Valley), and Texas. The vines are susceptible to powdery mildew caused by Sphaero-theca fuliginea (Schlecht. ex. Fr.) Poll, and the cucurbit mosaic viruses including papaya ringspot virus (watermelon mosaic virus, see ref. 3), watermelon mosaic virus 2, and zucchini yellow mosaic virus. Common quality defects of the fruit include traces of net, nonuniform shapes and sizes, low soluble solids, thin flesh, the cavity becoming watery prior to best edibility, and poor flavor. This report describes PMR Honeydew, a recently released powdery mildew resistant honeydew breeding line.

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Bertschi, S. "The Strong PMO." ITNOW 56, no. 2 (May 24, 2014): 12–14. http://dx.doi.org/10.1093/itnow/bwu036.

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Hellmann, Ch, and K. Müller. "PMR und Internet." Physikalische Medizin, Rehabilitationsmedizin, Kurortmedizin 08, no. 06 (December 1998): 211–13. http://dx.doi.org/10.1055/s-2008-1061848.

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Kang, Liping, Yan Zhang, Li Zhou, Jian Yang, Yali He, Shuai Yang, Gai Li, Qingxiu Hao, Yi Yu, and Lanping Guo. "Structural Characterization and Discrimination of Morinda officinalis and Processing Morinda officinalis Based on Metabolite Profiling Analysis." Frontiers in Chemistry 9 (January 21, 2022). http://dx.doi.org/10.3389/fchem.2021.803550.

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Morindae officinalis Radix (MOR) is a famous traditional Chinese medicine (TCM) and functional food material for invigorating kidneys and tonifying yang. Processed Morindae officinalis Radix (PMOR) is obtained by steaming MOR. Traditionally, the clinical effects are discrepant between processing and nonprocessing herbal medicines. MOR and PMOR are commonly used in both clinical practice and dietary supplements, and the effect of invigorating kidneys and tonifying yang of PMOR is stronger than MOR. To clarify the overall chemical composition and the difference of MOR and PMOR, a method was developed with an ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Among the 110 identified components shared by MOR and PMOR, 55 compounds showed significant differences in contents. Among them, the contents of 29 components, including fructooligosaccharides, monotropein, deacetylasperulosidic acid, geniposide, and anthraquinone glycosides, were higher in MOR than in PMOR; the contents of 26 components, including difructose anhydride sucrose, and iridoid glycoside derivatives, were higher in PMOR than in MOR. Difructose anhydrides and iridoid glycoside derivatives were first discovered in PMOR. These results provided a scientific basis for research on the therapeutic material basis of MOR. It would provide a method for the comparison of processing and nonprocessing in Chinese medicines.

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