Academic literature on the topic 'PlpD'
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Journal articles on the topic "PlpD"
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Trunk, Thomas, Michael A. Casasanta, Christopher C. Yoo, Daniel J. Slade, and Jack C. Leo. "Comparison of type 5d autotransporter phospholipases demonstrates a correlation between high activity and intracellular pathogenic lifestyle." Biochemical Journal 476, no. 18 (September 24, 2019): 2657–76. http://dx.doi.org/10.1042/bcj20190136.
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Abstract Autotransporters, or type 5 secretion systems, are widespread surface proteins of Gram-negative bacteria often associated with virulence functions. Autotransporters consist of an outer membrane β-barrel domain and an exported passenger. In the poorly studied type 5d subclass, the passenger is a patatin-like lipase. The prototype of this secretion pathway is PlpD of Pseudomonas aeruginosa, an opportunistic human pathogen. The PlpD passenger is a homodimer with phospholipase A1 (PLA1) activity. Based on sequencing data, PlpD-like proteins are present in many bacterial species. We characterized the enzymatic activity, specific lipid binding and oligomeric status of PlpD homologs from Aeromonas hydrophila (a fish pathogen), Burkholderia pseudomallei (a human pathogen) and Ralstonia solanacearum (a plant pathogen) and compared these with PlpD. We demonstrate that recombinant type 5d-secreted patatin domains have lipase activity and form dimers or higher-order oligomers. However, dimerization is not necessary for lipase activity; in fact, by making monomeric variants of PlpD, we show that enzymatic activity slightly increases while protein stability decreases. The lipases from the intracellular pathogens A. hydrophila and B. pseudomallei display PLA2 activity in addition to PLA1 activity. Although the type 5d-secreted lipases from the animal pathogens bound to intracellular lipid targets, phosphatidylserine and phosphatidylinositol phosphates, hydrolysis of these lipids could only be observed for FplA of Fusobacterium nucleatum. Yet, we noted a correlation between high lipase activity in type 5d autotransporters and intracellular lifestyle. We hypothesize that type 5d phospholipases are intracellularly active and function in modulation of host cell signaling events.
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Lim, K. P., Lisa F. P. Ng, and D. X. Liu. "Identification of a Novel Cleavage Activity of the First Papain-Like Proteinase Domain Encoded by Open Reading Frame 1a of the Coronavirus Avian Infectious Bronchitis Virus and Characterization of the Cleavage Products." Journal of Virology 74, no. 4 (February 15, 2000): 1674–85. http://dx.doi.org/10.1128/jvi.74.4.1674-1685.2000.
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ABSTRACT The coronavirus Avian infectious bronchitis virus (IBV) employs polyprotein processing as a strategy to express its gene products. Previously we identified the first cleavage event as proteolysis at the Gly673-Gly674 dipeptide bond mediated by the first papain-like proteinase domain (PLPD-1) to release an 87-kDa mature protein. In this report, we demonstrate a novel cleavage activity of PLPD-1. Expression, deletion, and mutagenesis studies showed that the product encoded between nucleotides 2548 and 8865 was further cleaved by PLPD-1 at the Gly2265-Gly2266 dipeptide bond to release an N-terminal 195-kDa and a C-terminal 41-kDa cleavage product. Characterization of the cleavage activity revealed that the proteinase is active on this scissile bond when expressed in vitro in rabbit reticulocyte lysates and can act on the same substrate intrans when expressed in intact cells. Both the N- and C-terminal cleavage products were detected in virus-infected cells and were found to be physically associated. Glycosidase digestion and site-directed mutagenesis studies of the 41-kDa protein demonstrated that it is modified by N-linked glycosylation at the Asn2313 residue encoded by nucleotides 7465 to 7467. By using a region-specific antiserum raised against the IBV sequence encoded by nucleotides 8865 to 9786, we also demonstrated that a 33-kDa protein, representing the 3C-like proteinase (3CLP), was specifically immunoprecipitated from the virus-infected cells. Site-directed mutagenesis and expression studies showed that a previously predicted cleavage site (Q2583-G2584) located within the 41-kDa protein-encoding region was not utilized by 3CLP, supporting the conclusion that the 41-kDa protein is a mature viral product.
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Lee, KiYoung, Dae-Won Kim, DoKyun Na, Kwang H. Lee, and Doheon Lee. "PLPD: reliable protein localization prediction from imbalanced and overlapped datasets." Nucleic Acids Research 34, no. 17 (September 11, 2006): 4655–66. http://dx.doi.org/10.1093/nar/gkl638.
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Lawlor, P. G., C. L. Nekolaichuk, S. S. Lowe, A. Kelly, R. L. Fainsinger, S. Watanabe, and E. D. Bruera. "Pre-admission escalation rate of daily opioid consumption (PERDOC), and total morphine equivalent daily dose on the first complete day of admission (D1-MEDD) to a tertiary-level palliative care unit (TPCU): Correlates and predictors in patients with advan." Journal of Clinical Oncology 25, no. 18_suppl (June 20, 2007): 9065. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.9065.
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9065 Background: Although animal laboratory studies attest to opioid tolerance (OT), it can be difficult in clinical practice to determine whether substantive escalation in opioid dose (average of >5% per day between initial daily dose and maximum daily dose to date) is due to disease progression (DP) or decreased opioid responsiveness, which in turn may be due to OT or other factors. Our study aims were to determine (1) the frequency of PERDOC>5%, (2) the correlates and predictors of PERDOC>5% and D1-MEDD. Methods: We retrospectively examined TPCU patient database records for demographics, physician rating of PERDOC in the Edmonton Staging System (ESS) for cancer pain classification, Edmonton Symptom Assessment System (ESAS) scores, and D1-MEDD. Consecutive 1st admission data on patients surviving >3 days were included in the initial analysis. Using complete data, logistic regression and multiple regression models were created with PERDOC>5% and logn D1-MEDD, respectively, as dependent variables. Results: From 1,351 patients who met the initial descriptive analysis eligibility criteria, 1212 (90%) had an ESS rating for PERDOC. The prevalence of PERDOC>5% was 274/1212 (19.3%). Bivariate analysis (N=969, complete data) showed that PERDOC>5% was positively associated (p<0.05) with younger age, neuropathic pain component (NPC), a pathological level of psychological distress (PLPD), substance abuse, higher D1-MEDD, and higher ESAS pain score (ESAS-P). In the multivariate analysis, NPC (Odds ratio: 2.1, 95% confidence interval: 1.5–2.9), PLPD (1.7, 1.2–2.6), and higher D1-MEDD (2.2, 1.03–4.7) were the strongest independent positive predictors, and ESAS-P (1.01, 1.003–1.02) remained as a weaker predictor. In the D1-MEDD regression model, positive predictors (p<0.05) were younger age, NPC, incident pain, PERDOC>5%, PLPD, ESAS-P and ESAS anxiety scores. Conclusions: Aside from OT and DP, the multiple predictors identified for PERDOC>5% and D1-MEDD underscore the need for a systematic multidimensional assessment of cancer pain that incorporates psychological and physical characteristics. No significant financial relationships to disclose.
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Canioni, Danielle, Nizar Mahlaoui, Chantal Andriamanga, Catherine Dubois d'Enghien, Jean-Philippe Jais, Alain Fischer, Olivier Hermine, Nicole Brousse, Dominique Stoppa-Lyonnet, and Felipe Suarez. "Histological Characteristics of Ataxia Telangiectasia Associated Lymphoproliferative Diseases. Results of the French Registry of Primary Immune Deficiencies." Blood 124, no. 21 (December 6, 2014): 1634. http://dx.doi.org/10.1182/blood.v124.21.1634.1634.
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Abstract Introduction Ataxia-telangiectasia (A-T) is a rare autosomal recessive disorder associated with mutations in the ATMgene and characterized by cerebellar ataxia, telangiectasia, immune defect, and a high incidence of lymphoid and solid cancers. We conducted a retrospective study of the patients with AT enrolled in the registry of the French National Reference Center for Primary Immune Deficiencies, in order to describe the incidence, subtypes, and outcomes of cancer and mainly of lymphoproliferative diseases (LPD) occurring in AT. Most of the LPD of this study were centrally reviewed by 2 expert hematopathologists. Patients & Results Sixty nine patients with cancers were identified among the 279 patients with AT of our cohort. Eight patients developed carcinomas, 8 acute leukemias, 3 T-cell prolymphocytic leukemias and 50 developed lymphomas. Among the lymphomas, 12 were classical Hodgkin's lymphomas (cHL), and 38 high-grade non-Hodgkin lymphomas (NHL, 26 of B-cell type, 4 of T-cell type, and 8 not phenotyped). We obtained a centralized histopathology review in 31 cases classified by pathology reports as cHL (n=6) and high-grade NHL (n=25). Cases were reviewed according to the WHO classification by H&E staining and immunohistochemistry. The presence of EBV was analyzed using antibodies to LMP and in situ hybridization for EBER. Concordance between the diagnosis established on pathology reports and the centralized review was excellent. All 6 cases of cHL were confirmed by the pathology review. The 25 cases initially diagnosed as high-grade NHL based on pathology reports fell into several WHO classification categories. Six corresponded to Burkitt's lymphomas (BL) and 12 to diffuse large B-cell lymphomas (DLBCL). Among the latter, 10 could be further classified according to cell-of-origin by the Hans algorithm as germinal center (GC, 2) and non-GC (8). Seven cases displayed polymorphic histological features reminiscent of post-transplant lymphoproliferative disorders (LPD), 5 polymorphic B cell LPD (pLPD), 1 with features of infectious mononucleosis (IM), another with features of plasmacytic hyperplasia LPD. EBV could be evaluated in 28 cases. All 6 cHL, 4 out of 11 DLBCL, all 5 pLPD, and the case of IM-like LPD were EBV positive by LMP and/or EBER staining. The 5 cases of BL that were evaluated were all EBV negative. Median age at diagnosis was 7.8 years [range 3 – 24], 14.9 [5.8 – 17.1], 12.2 [5.4 – 29] and 11 [6.3 – 17.7] for pLPD, cHL, BL and DLBCL respectively (p=ns). Median survival after diagnosis of lymphoma was 35, 8.6, 6 and 8 months for pLPD, cHL, BL and DLBCL respectively (p=ns). ATM mutation analysis was available for 20 cases (8 hypomorphic mutations, 12 loss-of-function mutations). There were no differences in age at diagnosis of lymphoma or survival according to the ATM mutation class or the EBV status. Conclusion B-cell malignancies including cHL and B-cell NHL are the most frequent malignancies in A-T and can be further classified as DLBCL, BL and cases with polymorphic features resembling post-transplant LPD. EBV seems to be associated with all cHL, approximately 50% of DLBCL and polymorphic LPD, but not with BL. The majority of DLBCL are non-GC type by immunohistochemistry. On this series of 31 patients, ATM mutation type was not associated with differences in type of lymphoma or outcome. Disclosures No relevant conflicts of interest to declare.
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Zhong, Zhi-Rong, Zhi-rong Zhang, Ji Liu, Yong Deng, Hong-wei Zhang, Yao Fu, Qing-guo Song, and Qin He. "Characteristics comparison before and after lyophilization of transferrin modified procationic- liposome- protamine- DNA complexes (Tf- PLPD)." Archives of Pharmacal Research 30, no. 1 (January 2007): 102–8. http://dx.doi.org/10.1007/bf02977785.
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Chen, Zhongbin, Yanhua Wang, Kiira Ratia, Andrew D. Mesecar, Keith D. Wilkinson, and Susan C. Baker. "Proteolytic Processing and Deubiquitinating Activity of Papain-Like Proteases of Human Coronavirus NL63." Journal of Virology 81, no. 11 (March 28, 2007): 6007–18. http://dx.doi.org/10.1128/jvi.02747-06.
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ABSTRACT Human coronavirus NL63 (HCoV-NL63), a common human respiratory pathogen, is associated with both upper and lower respiratory tract disease in children and adults. Currently, no antiviral drugs are available to treat CoV infections; thus, potential drug targets need to be identified and characterized. Here, we identify HCoV-NL63 replicase gene products and characterize two viral papain-like proteases (PLPs), PLP1 and PLP2, which process the viral replicase polyprotein. We generated polyclonal antisera directed against two of the predicted replicase nonstructural proteins (nsp3 and nsp4) and detected replicase proteins from HCoV-NL63-infected LLC-MK2 cells by immunofluorescence, immunoprecipitation, and Western blot assays. We found that HCoV-NL63 replicase products can be detected at 24 h postinfection and that these proteins accumulate in perinuclear sites, consistent with membrane-associated replication complexes. To determine which viral proteases are responsible for processing these products, we generated constructs representing the amino-terminal end of the HCoV-NL63 replicase gene and established protease cis-cleavage assays. We found that PLP1 processes cleavage site 1 to release nsp1, whereas PLP2 is responsible for processing both cleavage sites 2 and 3 to release nsp2 and nsp3. We expressed and purified PLP2 and used a peptide-based assay to identify the cleavage sites recognized by this enzyme. Furthermore, by using K48-linked hexa-ubiquitin substrate and ubiquitin-vinylsulfone inhibitor specific for deubiquitinating enzymes (DUBs), we confirmed that, like severe acute respiratory syndrome (SARS) CoV PLpro, HCoV-NL63 PLP2 has DUB activity. The identification of the replicase products and characterization of HCoV-NL63 PLP DUB activity will facilitate comparative studies of CoV proteases and aid in the development of novel antiviral reagents directed against human pathogens such as HCoV-NL63 and SARS-CoV.
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Gadlage, Mark J., and Mark R. Denison. "Exchange of the Coronavirus Replicase Polyprotein Cleavage Sites Alters Protease Specificity and Processing." Journal of Virology 84, no. 13 (April 28, 2010): 6894–98. http://dx.doi.org/10.1128/jvi.00752-10.
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ABSTRACT Coronavirus nonstructural proteins 1 to 3 are processed by one or two papain-like proteases (PLP1 and PLP2) at specific cleavage sites (CS1 to -3). Murine hepatitis virus (MHV) PLP2 and orthologs recognize and cleave at a position following a p4-Leu-X-Gly-Gly-p1 tetrapeptide, but it is unknown whether these residues are sufficient to result in processing by PLP2 at sites normally cleaved by PLP1. We demonstrate that exchange of CS1 and/or CS2 with the CS3 p4-p1 amino acids in engineered MHV mutants switches specificity from PLP1 to PLP2 at CS2, but not at CS1, and results in altered protein processing and virus replication. Thus, the p4-p1 residues are necessary for PLP2 processing but require a specific protein or cleavage site context for optimal PLP recognition and cleavage.
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Kanjanahaluethai, Amornrat, Dalia Jukneliene, and Susan C. Baker. "Identification of the Murine Coronavirus MP1 Cleavage Site Recognized by Papain-Like Proteinase 2." Journal of Virology 77, no. 13 (July 1, 2003): 7376–82. http://dx.doi.org/10.1128/jvi.77.13.7376-7382.2003.
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ABSTRACT The replicase polyprotein of murine coronavirus is extensively processed by three proteinases, two papain-like proteinases (PLPs), termed PLP1 and PLP2, and a picornavirus 3C-like proteinase (3CLpro). Previously, we established a trans-cleavage assay and showed that PLP2 cleaves the replicase polyprotein between p210 and membrane protein 1 (MP1) (A. Kanjanahaluethai and S. C. Baker, J. Virol. 74:7911-7921, 2000). Here, we report the results of our studies identifying and characterizing this cleavage site. To determine the approximate position of the cleavage site, we expressed constructs that extended various distances upstream from the previously defined C-terminal end of MP1. We found that the construct extending from the putative PLP2 cleavage site at glycine 2840-alanine 2841 was most similar in size to the processed MP1 replicase product generated in a trans-cleavage assay. To determine which amino acids are critical for PLP2 recognition and processing, we generated 14 constructs with amino acid substitutions upstream and downstream of the putative cleavage site and assessed the effects of the mutations in the PLP2 trans-cleavage assay. We found that substitutions at phenylalanine 2835, glycine 2839, or glycine 2840 resulted in a reduction in cleavage of MP1. Finally, to unequivocally identify this cleavage site, we isolated radiolabeled MP1 protein and determined the position of [35S]methionine residues released by Edman degradation reaction. We found that the amino-terminal residue of MP1 corresponds to alanine 2841. Therefore, murine coronavirus PLP2 cleaves the replicase polyprotein between glycine 2840 and alanine 2841, and the critical determinants for PLP2 recognition and processing occupy the P6, P2, and P1 positions of the cleavage site. This study is the first report of the identification and characterization of a cleavage site recognized by murine coronavirus PLP2 activity.
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Durie, Ian A., John V. Dzimianski, Courtney M. Daczkowski, Jack McGuire, Kay Faaberg, and Scott D. Pegan. "Structural insights into the interaction of papain-like protease 2 from the alphacoronavirus porcine epidemic diarrhea virus and ubiquitin." Acta Crystallographica Section D Structural Biology 77, no. 7 (June 18, 2021): 943–53. http://dx.doi.org/10.1107/s205979832100509x.
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Porcine epidemic diarrhea is a devastating porcine disease that is caused by the alphacoronavirus porcine epidemic diarrhea virus (PEDV). Like other members of the Coronaviridae family, PEDV encodes a multifunctional papain-like protease 2 (PLP2) that has the ability to process the coronavirus viral polyprotein to aid in RNA replication and antagonize the host innate immune response through cleavage of the regulatory proteins ubiquitin (Ub) and/or interferon-stimulated gene product 15 (ISG15) (deubiquitination and deISGylation, respectively). Because Betacoronavirus PLPs have been well characterized, it was sought to determine how PLP2 from the alphacoronavirus PEDV differentiates itself from its related counterparts. PEDV PLP2 was first biochemically characterized, and a 3.1 Å resolution crystal structure of PEDV PLP2 bound to Ub was then solved, providing insight into how Alphacoronavirus PLPs bind to their preferred substrate, Ub. It was found that PEDV PLP2 is a deubiquitinase and readily processes a variety of di-Ub linkages, in comparison with its Betacoronavirus counterparts, which have a narrower range of di-Ub activity but process both Ub and ISG15.
Dissertations / Theses on the topic "PlpD"
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Laubier, Aurélie. "Caractérisation et implication dans la pathogénicité de deux "Patatin-Like Proteins" de Pseudomonas Aeruginosa, PlpA ET PlpD." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4040.
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Durant ma thèse, nous avons identifier PlpA comme une cytotoxine conservée dans des isolats cliniques d'origines diverses, contrairement à son homologue, le facteur de virulence ExoU de Pseudomonas aeruginosa. Un rôle dans la cytotoxicité envers des cellules phagocytaires de l'immunité innée a été attribué à PlpA, et celui-ci dépend de l'intégrité de la dyade catalytique Ser/Asp, caractéristique des protéines de la famille des patatines. Un interactome réalisé in vivo dans des cellules hôtes nous a permis d'identifier des transporteurs de la mitochondrie comme partenaires de PlpA. L'interaction de PlpA avec ses partenaires mitochondriaux, aurait de manière inattendue un effet anti-apoptotique sur les macrophages, mais conduit cependant, à la mort de ceux-ci vraisemblablement par un phénomène de nécrose induite.PlpD a précédemment été caractérisée par Salacha et ses collaborateurs comme étant l'archétype du Système de Sécrétion de Type Vd (2010). Bien que le mécanisme précis de sécrétion de cette protéine reste à ce jour mal connu, nos travaux ont permis de lui attribuer un rôle dans la compétition bactérienne, conférant ainsi un avantage compétitif aux souches qui la possède. D'ailleurs, l'analyse phylogénétique de PlpD (Salacha et al., 2010 ; Heinz & Lithgow 2014) révèle la conservation de cette protéine au sein de nombreuses espèces vivants dans un environnement hostile, suggérant ainsi la nécessité de cette protéine dans l'implantation et la conservation de niches écologiques, que se soit dans l'environnement ou au cours d'infections polymicrobiennes chez un organisme hôteDuring my PhD, in the PAO1 strain of Pseudomonas aeruginosa, we identified PlpA as a cytotoxin conserved in clinical isolates of various origins, contrary to its virulence factor ExoU homologues. A cytotoxic role of PlpA has been highlighted against phagocytic cells, and showed to depend on the integrity of its Ser/Asp catalytic dyad. An in vivo interactome allowed us to identify mitochondrial transporters as partners of PlpA. Interestingly, PlpA interaction with these partners has an anti-apoptotic effect on macrophages but ultimely allows macrophages death probably by a necroptosis phenomenon. PlpD was previously described by Salacha and collaborators as the SST5d archetype (Salacha et al., 2010). While its exact secretion mechanism remains poorly understood, our work allowed showing that it played a role in bacterial competition. PlpD phylogenetic analysis (Salacha et al., 2010 ; Heinz & Lithgow 2014) revealed its conservation in many species living in hostile environments, suggesting its necessity in the implantation and conservation of ecological niches in the environment or during polymicrobial infections into host organism
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Madeira, Paulo Vinicius da Mata 1989. "Determinação da estrutura tridimensional do domínio catalítico do fator de virulência PlpD de Pseudomonas aeruginosa." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316461.
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Orientadores: Andréa Dessen de Souza e Silva, David NevesDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-25T14:11:04Z (GMT). No. of bitstreams: 1 Madeira_PauloViniciusdaMata_M.pdf: 3508240 bytes, checksum: 40070baffe8469acd5f3a60f9e82a931 (MD5) Previous issue date: 2014
Resumo: Segundo a Organização Mundial da Saúde, doenças infecciosas são a segunda principal causa de morte no mundo. Essas doenças são causadas por organismos patogênicos que podem compartilhar certas similaridades em seu modo de infecção. Bactérias patogênicas são responsáveis por diversas doenças de acometimento humano, sua patogenicidade, na maioria das vezes, apresenta-se associada a secreção de fatores de virulência que são responsáveis pela adesão, invasão e por danos ás células e tecidos do hospedeiro. P. aeruginosa é um patógeno oportunista, multiresistente a antibióticos e é a bactéria Gram negativa principal causadora de infecções hospitalares, podendo levar à óbito por pneumonia e sepse pacientes imunocomprometidos, principalmente pacientes com fibrose cística, AIDS e vítimas de queimadura. A proteína ExoU de P. aeruginosa é um fator de virulência, altamente citotóxico, secretado pela bactéria que apresenta atividade fosfolipase A, degradando a membrana celular e levando a rápida morte celular. ExoU é pertencente a família das proteínas tipo patatina, essas proteínas apresentam regiões homólogas a fosfolipase A2 citosólica humana e regiões homólogas a proteínas patatinas. A proteína PlpD encontrada em linhagens que não codificam ExoU, a saber: PA01 e PA14 de P. aeruginosa apresentam todas as regiões conservadas, classificando-a como uma proteína bacteriana do tipo patatina, assim como a ExoU. Além disso foi mostrado que seu domínio catalítico é secretado pela bactéria e apresenta atividade de lipase, importante para o processo infectivo do patógeno. Como P. aeruginosa, assim como outros patógenos, se tornaram multiresistentes a antibióticos, a busca por novos alvos terapêuticos vem sendo incentivada. A compreensão estrutural dos componentes envolvidos no processo infectivo é essencial para o desenvolvimento de novos agentes terapêuticos. Nesse trabalho a estrutura da porção secretada da proteína PlpD, com atividade catalítica, foi resolvida à uma resolção de 2.14 Angstroms. A análise da proteína mostrou diferenças interessantes entre sua estrutura e de seu homólogo ExoU, fornecendo pistas para a caracterização de seu mecanismo de ação à nível estrutural. Essa tese foi desenvolvida em colaboração com o grupo do Laboratório de Engenharia de Sistemas Macromoleculares de Marselha, França sob coordenação da Drª Sophie Bleves
Abstract: According to the World Health Organization, infectious diseases are the second leading cause of deaths worldwide . These diseases are caused by pathogenic organisms that may share certain similarities in their mode of infection. Pathogenic bacteria are responsible for many human diseases, their pathogenicity, most often appears associated with the secretion of virulence factors that are responsible for adhesion, invasion and damage to the cells and tissues of the host. P. aeruginosa is an opportunistic pathogen, multidrug-resistant and the main Gram negative cause of nosocomial infections, this pathogen may lead to death due to pneumonia and septcemia immunocompromised patients, especially patients with cystic fibrosis, AIDS and burn victims. The ExoU protein of P. aeruginosa is a highly cytotoxic virulence factor secreted by the bacterium which has phospholipase A activity by degrading the cell membrane and leading to rapid cell death. ExoU belongs to patatin-like protein family, these proteins have regions homologous to human cytosolic phospholipase A2 and regions homologous to patatins. The PlpD protein is found in strains that do not encode ExoU, namely P. aeruginosa PA01 and PA14. This protein shows all conserved regions of patatin-like proteins, classifying it as a bacterial patatin-like protein, as well as the ExoU. Furthermore it was shown that its catalytic domain is secreted by the bacterium and shows lipase activity, important for the infection process. As P. aeruginosa, as well as other pathogens have become multidrug resistant, the search for new therapeutic targets is being encouraged. The understanding of the structural components involved in the infective process is essential for the development of new therapeutic agents. In this work the structure of the secreted portion of PlpD protein which has catalytic activity was resolved at 2.14 angstroms resolution. The protein analysis showed interesting differences between its structure and its homologous ExoU, providing evidences to the characterization of its mechanism of action at a structural level. This thesis was developed in collaboration with the Macromolecular Engineering Systems Laboratory group in Marseille, France under the coordination of Dr Sophie Bleves
Mestrado
Genetica de Microorganismos
Mestre em Genética e Biologia Molecular
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Salacha, Richard. "Les Patatines de Pseudomonas Aeruginosa : secrétées ou non secrétées ? Telle est la question." Thesis, Aix-Marseille 2, 2010. http://www.theses.fr/2010AIX22040/document.
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Pseudomonas aeruginosa est une bactérie à Gram négatif ubiquitaire, pathogène opportuniste. Elle est la 3ème cause d’infections nosocomiales, notamment chez les immunodéprimés et les grands brûlés. Elle est aussi responsable de la mort de nombreux patients atteints de la mucoviscidose. Sa virulence est largement due à son aptitude à sécréter de nombreuses enzymes dégradatives et toxines, parmi lesquelles la protéine ExoU, sécrétée par le Système de Sécrétion de Type III. ExoU est une phospholipase, de la famille des « patatin-like proteins », dont l’activité est portée par une dyade catalytique Ser-Asp.Mon travail de thèse a permis d’identifier 4 homologues d’ExoU (PlpA, PlpB, PlpC et PlpD) dans le protéome de la souche PAO1 de P. aeruginosa (qui est dépourvu de cette protéine). En étudiant le mode de sécrétion de PlpD, nous avons découvert une nouvelle branche du Système de Sécrétion de Type V (SST5), le SST5d. La protéine représentant ce nouveau système possède un domaine C-terminal transporteur de type TpsB (SST5b), fusionné à un domaine N-terminal patatine sécrété dans le milieu extracellulaire (à l’image d’un autotransporteur, ou SST5a). Ce mode de sécrétion serait un mode dédié à la sécrétion de « patatin-like proteins », comme le suggèrent nos analyses phylogénétiques, à Nous avons en outre démontré que PlpD possède une activité lipase.L’autre protéine étudiée, PlpA, est également sécrétée, bien que nous n’ayons pu établir avec certitude sa voie de transport. Nous avons évalué le rôle de cette protéine lors de l’interaction de P. aeruginosa avec des cellules hôtes de type macrophages et cellules épithéliales. Nous avons observé que cette protéine confère une protection temporaire aux cellules infectées par P. aeruginosa. Ce retard semble être directement imputable à l’activité de la protéine, puisqu’il est dépendant de l’intégrité de la dyade catalytique putative de PlpAPseudomonas aeruginosa is an ubiquitous Gram negative bacteria, and efficient opportunistic pathogen. It is the third most common cause of nosocomial infections, most particularly within immunocompromized or burn patients. This pathogen is responsible for the death of numerous cystic fibrosis patients. Its virulence is due mainly to its capacity to secrete numerous degradative enzymes and toxins, among them, ExoU which is secreted via the Type III Secretion System. ExoU is a phospholipase of the patatin-like protein family, and its activity is based on a Ser-Asp catalytic dyad.During my thesis, we identify 4 ExoU homologs (PlpA, PlpB, PlpC, and PlpD) in the proteome of the P. aeruginosa PAO1 strain (this strain does not possess ExoU). Results obtained studying PlpD secretion led us to discover a new branch of the Type V Secretion System (T5SS), the T5dSS. PlpD is composed of a C-terminal TpsB-like transporter domain (like T5bSS), fused to a N-terminal patatin domain which is secreted into the extracellular medium (like autotransporters, or T5aSS). Our phylogenetic analysis suggests that this secretion pathway may be dedicated to the secretion of PLPs, like T5cSS, which secretes only adhesins. Moreover, we demonstrated that PlpD is a lipase.The other studied protein, PlpA, is also a secreted protein, but we still do not know which secretion system is involved in its secretion. We tested the role of PlpA during interaction of P. aeruginosa with host cells by carrying out infections of murin macrophages and epithelial cells. We observed a transitory protection of cells infected with P. aeruginosa. This protection seems to require an active PlpA protein as it is dependent on a intact catalytic dyad in this protein
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Ayala, Carlos A. "PLED Enhancement and Re-use." DigitalCommons@CalPoly, 2010. https://digitalcommons.calpoly.edu/theses/419.
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Polymer light emitting diodes (PLEDs) represent new technology for display applications. However, these polymer based devices could benefit from increased efficiency and material longevity.
This thesis examines how preprocessing PLED indium tin oxide (ITO) anodes and reprocessing previously used substrates can create brighter, more efficient PLEDs. The project accomplishes this by simple changes to fabrication techniques, such as additional cleaning, etching, or thermal annealing, to improve pristine device luminance and efficiency. The project also examines substrate re-use techniques to repair ITO substrate damage, and effects of polymer aging on PLED luminance and efficiency.
PLEDs fabricated with polymer aged to varying degrees exhibit increased efficiency and luminance. Etching previously used substrates allows PLED re-use; substrates etched in hydrochloric acid also demonstrate increased efficiency and luminance.
Preprocessing improves device performance, while etching results in reusable substrates.
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Junior, Jose Antonio Portes. "Detecção da proteína PLP2 em glioblastomas." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-29092010-153428/.
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Recentemente, com o intuito de identificar genes associados com invasão e proliferação tumoral, identificamos por PCR em tempo real, um aumento de aproximadamente cem vezes da proteína PLP2 em glioblastomas em relação a tecidos normais. Até o momento não há nenhum relato da identificação desta proteína em astrocitomas. Portanto, neste trabalho clonamos e expressamos em bactérias, as alças externas da PLP2 em fusão com a proteína SUMO, com o objetivo de obtermos anticorpos policlonais para serem usados na identificação da PLP2 em tumor humano por western blotting. Realizamos também a expressão da PLP2 fusionada com a EGFP em células de mamífero, para estudar sua distribuição celular, observamos que a PLP2 se concentra em toda a membrana celular e estudos sobre o transito da PLP2 nas células, indicam que ela possa estar envolvida em processos quimiotáticos via CCR1 sugerindo o envolvimento da PLP2 de alguma forma no processo tumorigênico.Recently, in order to identify genes associated with tumoral invasion and proliferation, identified by real time PCR, an increase of about one hundred times of PLP2 protein in glioblastomas when compared to normal tissue. So far, there is no report of identification of this protein in astrocytomas. Therefore in this study, we cloned and expressed in bacteria the external handles of PLP2 fused with SUMO protein in order to obtain polyclonal antibodies for use in identifying the PLP2 in human tumor by western blotting. We also expressing the PLP2 fused with EGFP in mammalian cells to study its cellular distribution, we observed that focuses PLP2 across the cell membrane and studies on the traffic of PLP2 cells, indicate that it may be involved in chemotactic processes via CCR1 suggesting the involvement of PLP2 somehow in the tumorigenic process.
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Zimmermann, Gregor. "Elektrische Charakterisierung PLD-gewachsener Zinkoxid-Nanodrähte." Doctoral thesis, Universitätsbibliothek Leipzig, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-61365.
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Die vorliegende Arbeit beschäftigt sich mit der elektrischen Charakterisierung von Zinkoxid-Nanodrähten, die mittels gepulster Laserablation (PLD) hergestellt wurden.
Ausgehend von den so generierten ZnO-Nanodraht-Ensembles werden Methoden zu deren elektrischer Untersuchung diskutiert und auf praktische Anwendbarkeit hin verglichen. Die entwickelten Methoden werden auf Ensembles von auf n-leitenden ZnO- und ZnO:Ga-Dünnschichten aufgewachsenen Phosphor-dotierten ZnO-Nanodrähten angewendet. Deren reproduzierbares, in Strom–Spannungs- (I–U-) Kennlinien beobachtetes diodenartiges Verhalten wird genauer beleuchtet.
Im Zusammenhang mit der elektrischen Charakterisierung einzelner ZnO-Nano-drähte werden experimentelle Methoden zur Vereinzelung und zur Kontaktierung der vereinzelten ZnO-Nanodrähte diskutiert. Dabei werden sowohl etablierte Methoden wie Elektronenstrahllithographie (EBL) als auch neue Techniken wie elektronen- und ionenstrahlinduzierte Deposition (EBID/IBID) und Strom–Spannungs-Rastersondenmikroskopie (I-AFM) behandelt und ihre Eignung für eingehende elektrische Untersuchungen und reproduzierbare Messungen analysiert.
Die geeignetsten Methoden werden schließlich eingesetzt, um spezifischen Widerstand sowie Ladungsträgermobilität und -dichte sowohl in nominell undotierten als auch in Aluminium-dotierten ZnO-Nanodrähten zu untersuchen und zu vergleichen. In der Ableitung der physikalischen Materialparameter aus den Messdaten wird dabei besonderes Augenmerk auf die Einbeziehung der geometrischen Besonderheiten der Nanodrähte gegenüber Volumenmaterial- und Dünnschichtproben gelegt. Im Zuge dessen wird unter anderem ein Modell für den elektrischen Widerstand in Nanodrähten mit ihrer Länge nach veränderlichem Querschnitt abgeleitet.
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Correia, Fábio Conte. "Síntese e caracterização de polímeros contendo 9,9-dioctilfluoreno e 8-oxioctilquinolina para utilização como camada emissora de PLEDs." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/3/3133/tde-11072014-115535/.
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Utilizando a reação de acoplamento de Suzuki, novos polímeros e copolímeros semicondutores com elevado potencial para a fabricação de PLEDs foram sintetizados, tendo como finalidade unir em uma única camada emissiva, na forma de copolímeros ou na forma de blendas, materiais com propriedades transportadoras e injetoras de elétrons; grupos quinolina associados a materiais com elevadas propriedades de emissão de luz contendo grupos fluoreno. Esses copolímeros sintetizados, todos ainda não descritos na literatura ou em patentes, apresentaram rendimentos acima de 70% e rendimentos quânticos de fotoluminescência entre 60% e 83%, foram utilizados como camada emissiva na construção de PLEDs. Estes PLEDs foram caracterizados quanto ao seu comportamento elétrico através da obtenção de curvas de corrente em função da tensão (IxV) e dos espectros de eletroluminescência. Os resultados mostraram que a incorporação da quinolina aos copolímeros aumentou a sua estabilidade térmica, observada pela temperatura de inicio de degradação que elevou-se em até 80°C. Nos PLEDs, houve melhorias no balanceamento de cargas, dispensando até mesmo a deposição de uma camada adicional transportadora de elétrons (ETL). As tensões de operação desses dispositivos ficaram entre 2,0 e 5,2 V, com emissão entre 525 e 590nm. Esses materiais também tiveram as suas estruturas caracterizadas por ressonância magnética nuclear de hidrogênio, termogravimetria, calorimetria diferencial exploratória, espectroscopias no UV-Vis e no infravermelho, fluorimetria no UV-vis e cromatografia de permeação em gel. Filmes Langmuir e Langmuir- Blodgett dos copolímeros foram preparados e caracterizados por espectroscopia com luz polarizada de reflexão e absorção no infravermelho (PM-IRRAS) e por microscopia de força atômica (AFM).New polymers and copolymers with a high potential for PLEDs constructions have been synthesized by Suzuki reaction and aims together in a single emissive layer in the form of copolymers or blended, materials with transporting and electron injection properties; quinoline groups linked to materials with high light emission properties as fluorene group. All these copolymers have not been described in literature or in patents, presented yields above 70%, quantum yields between 60% and 83% and were used as emissive layer in PLEDs. These PLEDs were characterized concerning their electrical behavior, by the characteristic J-V diode curves, and their electroluminescence. The results showed that the presence of quinoline increased its thermal stability at around 80° C and the PLEDs built with the synthesized copolymers do not need an extra ETL. The operating voltages of these devices were observed between 2.0 and 5.2 V with EL emission between 525 and 590nm. These new materials were also characterized by hydrogen nuclear magnetic resonance, thermogravimetry, differential scanning calorimetry, UV-Vis, Fluorescence and IR spectroscopy and gel permeation chromatography. Langmuir e Langmuir-Blodgett films were made and characterized by Polarization-Modulation Infrared Reflection-Absorption Spectroscopy (PM-IRRAS) and atomic force microscopy (AFM).
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Valtavaara, M. (Minna). "Novel lysyl hydroxylase isoforms." Doctoral thesis, University of Oulu, 1999. http://urn.fi/urn:isbn:9514253221.
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Abstract
Lysyl hydroxylase (EC 1.14.11.4, procollagen-lysine 2-oxoglutarate 5-dioxygenase, PLOD) catalyzes the hydroxylation of lysine residues in collagens and other proteins. It occurs as a post-translational event. The hydroxylysine residues participate in the formation of covalent cross-links to stabilize the collagenous structure in tissues. The hydroxylysine residues can be glycosylated to galactosyl- or glucosylgalactosylhydroxylysine residues.
Novel human lysyl hydroxylases, 2a, 2b and 3 isoforms, were characterized in this study. Lysyl hydroxylases 2a and 2b are alternatively spliced forms of lysyl hydroxylase 2. Lysyl hydroxylase 2b contains an additional exon of 63 nucleotides. The polypeptide size of lysyl hydroxylase 2a is 737 amino acids, lysyl hydroxylase 2b is 758 amino acids and lysyl hydroxylase 3 is 738 amino acids. The putative signal peptide is 25 amino acids in lysyl hydroxylases 2a and 2band 24 amino acids in lysyl hydroxylase 3. Lysyl hydroxylases 2aand 2b contain 7 possible N-glycosylation sites and lysyl hydroxylase3 contains 2 sites.
Tissue distribution of novel isoforms were studied on Northern blots. The expression of lysyl hydroxylases 2a, 2b and 3 differ from the expression of previously characterized lysyl hydroxylase 1. Lysyl hydroxylase 1 is expressed constitutively in all tissues whereas the expression of novel isoforms is more strictly regulated. Lysyl hydroxylase 2 is highly expressed in heart, placenta, liver and pancreas. Lysyl hydroxylase 2b expression is highest in heart and skeletal muscle and lysyl hydroxylase 3 expression is highest in heart, placenta and pancreas. Brain, lung and kidney contain the lowest amounts of these isoforms.
Novel isoforms were expressed as recombinant proteins in baculovirus expression system in vitro. All these novel isoforms were able to hydroxylate lysine residues in short collagenous peptides. A more detailed kinetic analysis was performed on lysyl hydroxylase 2a and 2b in order to find out if they differed from each other. The binding affinity of ascorbate and peptide substrate is different in lysyl hydroxylase 2a from 2b.
Chromosomal assignments were carried out on human lysyl hydroxylases 2 and 3. Lysyl hydroxylase 2 was localized to chromosome 3q23–q24 and lysyl hydroxylase 3 to chromosome 7q36.
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Aletrari, Mina-Olga. "Characterisation of PLD activity in real-time." Thesis, University of Warwick, 2010. http://wrap.warwick.ac.uk/4479/.
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PLD catalyses hydrolysis of phosphatidylcholine (PtdCho) to produce phosphatidic acid (PtdOH) and choline. PtdOH is a second messenger responsible for a multitude of cell processes, ranging from cytoskeletal rearrangement to cell proliferation. Antigenic stimulation of RBL-2H3 mast cells and growth factor stimulation of endothelial HeLa cells results in PLD-dependent exocytosis and endocytosis, respectively. A novel fluorescent PtdCho (fPtdCho) was used to label both cell lines and Bligh-Dyer lipid extraction of fPtdCho-labelled RBL-2H3 cells showed the lipid was intact post-labelling. fPtdCho co-localised up to 50% with the lysosomal marker LysoTracker Red in RBL- 2H3 cells, and was not secreted in response to antigenic stimulation as recorded using real-time confocal microscopy. Primary alcohol treatment of fPtdCho-labelled RBL- 2H3 cells altered fPtdCho-labelling to diffuse from punctate distribution, suggesting PLD-generated PtdOH is responsible for retention of punctate fPtdCho staining. PLD isoforms 1b and 2a were labelled with Cherry (a red fluorescent protein) and transiently expressed in fPtdCho-labelled HeLa cells. Localisation was assessed using FRET by FRAP technology in live cells and showed that substrate and lipase were in close proximity. These findings will facilitate future development of a live real-time in vivo PLD assay. Furthermore, localisation of PLD and its activator Rac1 was assessed at rest and in EGF-stimulated HeLa cells in real-time. This showed co-localisation between PLD and Rac1 following stimulation. The fluorescent PtdCho was also used to develop a novel real-time in vitro PLD assay, monitoring fPtdCho metabolism at two second intervals. This in vitro assay is more sensitive than traditional end-point assays and will help clarify the relative rate of PLD activation in response to small G-protein activators and other co-factors in real-time.
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Yool, Donald Andrew. "Phenotypic analysis of the Plp-deficient mouse." Thesis, University of Glasgow, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312690.
Full textBooks on the topic "PlpD"
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Amy, Smith, and Twonky Tonette, eds. Percy & the plod. Soquel, Calif: FWE Pub., 2002.
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Le commentaire litte raire et l'explication de texte: Pour la pre paration aux PLP, PLPA, CAFEP et CAPES. Paris: Ellipses, 2007.
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Kira, Khudoleĭ, and Severi︠u︡khin D. I︠A︡, eds. Zapretnyĭ plod: Ėrotika v ėkslibrise. Moskva: Izdatelʹstvo Lomonosovʺ, 2010.
Find full textBook chapters on the topic "PlpD"
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Verma, Nitun, and Clete A. Kushida. "Restless Legs and PLMD." In Primary Care Sleep Medicine, 339–44. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1185-1_30.
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Neumann, Norbert J., and Percy Lehmann. "Polymorphe Lichtdermatose (PLD)." In Photodermatosen, 5–7. Heidelberg: Steinkopff, 2000. http://dx.doi.org/10.1007/978-3-662-12686-8_2.
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Wakaki, Toshiko, Katsumi Inoue, Chiaki Sakama, and Katsumi Nitta. "The PLP System." In Logics in Artificial Intelligence, 706–9. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-540-30227-8_62.
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Navabi, Zainalabedin. "PLD Based Design." In Digital Design and Implementation with Field Programmable Devices, 3–16. Boston, MA: Springer US, 2005. http://dx.doi.org/10.1007/1-4020-8012-3_1.
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Taraate, Vaibbhav. "Introduction to PLD." In PLD Based Design with VHDL, 169–209. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3296-7_6.
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Taraate, Vaibbhav. "Introduction to HDL." In PLD Based Design with VHDL, 1–22. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3296-7_1.
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Taraate, Vaibbhav. "Synthesis Optimization Using VHDL." In PLD Based Design with VHDL, 313–67. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3296-7_10.
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Taraate, Vaibbhav. "Design Implementation Using Xilinx Vivado." In PLD Based Design with VHDL, 369–94. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3296-7_11.
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Taraate, Vaibbhav. "Erratum to: PLD Based Design with VHDL." In PLD Based Design with VHDL, E1—E2. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3296-7_12.
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Taraate, Vaibbhav. "Basic Logic Circuits and VHDL Description." In PLD Based Design with VHDL, 23–47. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3296-7_2.
Full textConference papers on the topic "PlpD"
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Marumo, Masashi, and Nobuhisa Ito. "PLDD Update." In SPIE Proceedings, edited by Leonardo Longo, Alfons G. Hofstetter, Mihail-Lucian Pascu, and Wilhelm R. A. Waidelich. SPIE, 2004. http://dx.doi.org/10.1117/12.584389.
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De Maria, Letizia, Claudia Rinaldi, Piero Lupetin, and Valter Sergo. "Portable Optical System for In-Situ Thermal Barrier Assessment of Service Operated Blades." In ASME Turbo Expo 2006: Power for Land, Sea, and Air. ASMEDC, 2006. http://dx.doi.org/10.1115/gt2006-90551.
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Thermally Grown Oxide (TGO) layer develops and grows subjected to very high compressive residual stresses at the interface between metallic bond coat and EB-PVD thermal barrier coating. A significant decrease of such stress level is a useful indicator of incipient local detachment of the TBC. Photo-Stimulated Luminescence Piezo Spectroscopy (PLPS) provides information on the TGO stress state. In this paper a new portable PLPS instrument is described, developed to allow the in shop detection of PLPS signals on service operated blades with TBCs, to detect incipient failure. The instrument capability was verified comparing its results with the measurements performed with a commercial instrument on both TBC coated specimens aged in laboratory conditions and on new and operated components. The results confirm the system capability of prematurely detect and localise initial damage processes, before spallation of the TBC. A miniaturised probe was shaped to perform the PLPS measurement also at the leading edge. Finally the first in-shop application of the prototype is discussed starting from the results of the damage level assessment performed on operated first stage blades of a V94.3A2 gas turbine.
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Allison, James T. "Plant-Limited Co-Design of an Energy-Efficient Counterbalanced Robotic Manipulator." In ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/detc2012-71108.
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Modifying the design of an existing system to meet the needs of a new task is a common activity in mechatronic system development. Often engineers seek to meet requirements for the new task via control design changes alone, but in many cases new requirements are impossible to meet using control design only; physical system design modifications must be considered. Plant-Limited Co-Design (PLCD) is a design methodology for meeting new requirements at minimum cost through limited physical system (plant) design changes in concert with control system redesign. The most influential plant changes are identified to narrow the set of candidate plant changes. PLCD provides quantitative evidence to support strategic plant design modification decisions, including tradeoff analyses of redesign cost and requirement violation. In this article the design of a counterbalanced robotic manipulator is used to illustrate successful PLCD application. A baseline system design is obtained that exploits synergy between manipulator passive dynamics and control to minimize energy consumption for a specific pick-and-place task. The baseline design cannot meet requirements for a second pick-and-place task through control design changes alone. A limited set of plant design changes is identified using sensitivity analysis, and the PLCD result meets the new requirements at a cost significantly less than complete system redesign.
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Wei, Hsiu-Ping, Ming-Chih Yew, Chao-Jen Huang, and Kuo-Ning Chiang. "Failure Mode and Thermal Performance Analysis of Stacked Panel Level Package (PLP)." In ASME 2007 InterPACK Conference collocated with the ASME/JSME 2007 Thermal Engineering Heat Transfer Summer Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/ipack2007-33368.
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In this paper, a new packaging technology, chip-on-metal (COM) panel level package (PLP), with stacking and fan-out capabilities is proposed. Moreover, the concept of the COM PLP and the process of its fabrication are described. During the manufacturing process, the trench around the chip is filled with the filler polymer material. Therefore, the solder bumps could be located on both the filler polymer and the chip surfaces by the redistribution lines, and the pitch of the chip side is fanned-out. In our previous research, it was shown that the physical behavior of the COM PLP is different from that of the conventional wafer level package (WLP). To assess the thermal performance and thermo-mechanical characteristic of the proposed PLP, the finite element analysis (FEA) in board level is carried out. The junction temperature and thermal resistance of the COM PLP and the stacked PLP are discussed to study the thermal performance. At the same time, the mean cycle to failure of the solder joints is predicted, and the result shows that the reliability of solder joints can be highly improved by the proposed packaging technology. However, the new failure mode may occur at the metallic traces so the reliability assessment of the signal trace is also investigated. In addition, the parametric analysis of the COM PLP is studied to enhance the thermal performance and reliability characteristic. Thus, the PLP technology will have high potential for various applications in the near future.
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Priadi, Dedi, Margaretha Suliyanti, and Rusman Kosasih. "PLD Nd." In APCORISE 2020: 3rd Asia Pacific Conference on Research in Industrial and Systems Engineering 2020. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3400934.3400978.
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Yap, B. K., S. P. Koh, S. K. Tiong, and C. N. Ong. "Thermal stress test for PLED." In 2010 IEEE International Conference on Semiconductor Electronics (ICSE). IEEE, 2010. http://dx.doi.org/10.1109/smelec.2010.5549354.
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Gokdel, Y. D., A. O. Sevim, B. Kucukakarsu, S. Mutlu, and A. D. Yalcinkaya. "PLED integrated FR4 MEMS display." In LEOS 2009 -22nd Annuall Meeting of the IEEE Lasers and Electro-Optics Society (LEO). IEEE, 2009. http://dx.doi.org/10.1109/leos.2009.5343465.
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Chi, Xiao fei, Hong zhi Li, Ru zhou Wu, and Yun xian Sui. "Treatment of lumbar disc herniation by percutaneous laser disc decompression (PLDD) and modified PLDD." In 2004 Shanghai international Conference on Laser Medicine and Surgery, edited by Jing Zhu. SPIE, 2005. http://dx.doi.org/10.1117/12.639360.
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Hamadi, Rachid, Mathieu Allory, and Hayder Haouaneb. "PLD CPDL FPGA." In En avant toute ! La filière nucléaire innove pour exploiter le parc dans la durée. Les Ulis, France: EDP Sciences, 2019. http://dx.doi.org/10.1051/jtsfen/2019ena12.
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Vakhtin, Andrei B., Kristen A. Peterson, Daniel J. Kane, Eric H. Jordan, Geoffrey Hansen, and Matthew Teicholz. "Combination of Fourier-Domain Optical Coherence Tomography and Photo-Stimulated Luminescence Piezo-Spectroscopy as an NDE Tool for Thermal Barrier Coatings." In ASME Turbo Expo 2007: Power for Land, Sea, and Air. ASMEDC, 2007. http://dx.doi.org/10.1115/gt2007-27557.
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A combination of two optical methods — Fourier-domain optical coherence tomography (FD-OCT) and photo-stimulated luminescence piezo-spectroscopy (PLPS) is used as a non-destructive evaluation (NDE) tool for thermal barrier coatings (TBC). This research is focused on NDE of electron beam physical vapor deposition (EB-PVD) TBC’s. FD-OCT is an interferometric technique, which uses spectrally broadband visible or infrared light to obtain spectrally resolved interferograms of the light that is back-scattered from subsurface structures and defects (e.g., interfaces, cracks, voids) in optically translucent material. When the Fourier transform is applied to the interferogram, a depth-resolved image of the back-scattering sites is obtained. FD-OCT is shown to be a useful NDE tool that can profile the top coat-metal substrate interface and measure the top coat thickness. Also, it has the potential of assessing microcracking and spallation damage. PLPS provides quantitative information on stress in the thermally grown oxide (TGO) by measuring the spectral shifts in the laser-induced luminescence spectra of the Cr3+ ions present in the TGO. When combined, the PLPS and FD-OCT methods can provide a set of important input parameters for the TBC remaining life predicting model. Ultimately they will collect spatially resolved data on matching spatial domains. The two optical methods are applied to thermally cycled EB-PVD TBC samples. The experimental results are compared to destructive inspection data.
Reports on the topic "PlpD"
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McLean, W. II, E. Fehring, E. Dragon, and B. Warner. High rate PLD of diamond-like-carbon utilizing copper vapor lasers. Office of Scientific and Technical Information (OSTI), August 1994. http://dx.doi.org/10.2172/125098.
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Federal Information Processing Standards Publication: videotexteletext presentation level protocol syntax (North America plps). Gaithersburg, MD: National Bureau of Standards, 1986. http://dx.doi.org/10.6028/nbs.fips.121-1986.
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