Relevant bibliographies by topics / Platelet rich plasma scaffold / Journal articles

Journal articles on the topic 'Platelet rich plasma scaffold'

To see the other types of publications on this topic, follow the link: Platelet rich plasma scaffold.
Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Platelet rich plasma scaffold.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Mahanani, Erlina Sih, and Fannisa Afrilyana Ulzanah. "Efficacy of Incorporation Platelet Rich Plasma into Gelatine Hydrogel Scaffold between Impregnated and Drop Method." BIO Web of Conferences 41 (2021): 05002. http://dx.doi.org/10.1051/bioconf/20214105002.

Full text
Abstract:
Tissue Engineering which involve three main component such as scaffold, platelet-rich plasma (PRP) and cells is expected to support in bone regeneration. Gelatin hidrogel scaffold is planted have a function as cell environment and PRP provide growth factor to support differentiation of cells. The success of tissue engineering is affected by number of PRP which is contained in scaffold. The purpose of this study is to compare the incorporation process between impregnated and drop method to gelatin hidrogel scaffold. PRP was prepared from three donors of whole blood, and twice sentrifugation by 450 rcf for 5 minutes and 1500 rcf for 7 minutes. PRP was incorporated into 3 gelatin hidrogel scaffolds for each methods. The remnant of PRP which didn’t incorporate were calculated the number of platelet with giemsa stainning. Platelet which loaded were the reduction result of number platelet before incorporate with platelet remnant. Data of the result were analyzed using independent sample t test. Result show the significant was 0.262 (p>0.05) there’s no significane different between impregnated and drop method for incorporating PRP into gelatin hidrogel scaffold. The number of platelet which incorporated in gelatin hidrogel scaffold were effected by characteristic of scaffold such as structure, interface adherence, porosity and swelling ability. The good characteristic of scaffold could be obtain from synthesis and good fabrication technique.
APA, Harvard, Vancouver, ISO, and other styles
2

Shimojo, Andréa Arruda Martins, Amanda Gomes Marcelino Perez, Sofia Elisa Moraga Galdames, Isabela Cambraia de Souza Brissac, and Maria Helena Andrade Santana. "Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine." Scientific World Journal 2015 (2015): 1–12. http://dx.doi.org/10.1155/2015/396131.

Full text
Abstract:
This study aimed to evaluate thein vitroperformance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. The sponges were prepared by controlled freezing (−20, −80, or −196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v). The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum. The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma. The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells. The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a −20°C freezing temperature, while −196°C favored osteogenic differentiation. Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.
APA, Harvard, Vancouver, ISO, and other styles
3

Sancho-Tello, Maria, Sara Martorell, Manuel Mata Roig, Lara Milián, MA Gámiz-González, Jose Luis Gómez Ribelles, and Carmen Carda. "Human platelet-rich plasma improves the nesting and differentiation of human chondrocytes cultured in stabilized porous chitosan scaffolds." Journal of Tissue Engineering 8 (January 1, 2017): 204173141769754. http://dx.doi.org/10.1177/2041731417697545.

Full text
Abstract:
The clinical management of large-size cartilage lesions is difficult due to the limited regenerative ability of the cartilage. Different biomaterials have been used to develop tissue engineering substitutes for cartilage repair, including chitosan alone or in combination with growth factors to improve its chondrogenic properties. The main objective of this investigation was to evaluate the benefits of combining activated platelet-rich plasma with a stabilized porous chitosan scaffold for cartilage regeneration. To achieve this purpose, stabilized porous chitosan scaffolds were prepared using freeze gelation and combined with activated platelet-rich plasma. Human primary articular chondrocytes were isolated and cultured in stabilized porous chitosan scaffolds with and without combination to activated platelet-rich plasma. Scanning electron microscopy was used for the morphological characterization of the resulting scaffolds. Cell counts were performed in hematoxylin and eosin–stained sections, and type I and II collagen expression was evaluated using immunohistochemistry. Significant increase in cell number in activated platelet-rich plasma/stabilized porous chitosan was found compared with stabilized porous chitosan scaffolds. Chondrocytes grown on stabilized porous chitosan expressed high levels of type I collagen but type II was not detectable, whereas cells grown on activated platelet rich plasma/stabilized porous chitosan scaffolds expressed high levels of type II collagen and type I was almost undetectable. In summary, activated platelet-rich plasma increases nesting and induces the differentiation of chondrocytes cultured on stabilized porous chitosan scaffolds.
APA, Harvard, Vancouver, ISO, and other styles
4

Li, Muxin, Rubina Rahaman Khadim, Mitsuru Nagayama, Marie Shinohara, Kousuke Inamura, Mathieu Danoy, Masaki Nishikawa, Katsuko Furukawa, Yasuyuki Sakai, and Toshiki Niino. "Fabrication of a Porous Three-Dimensional Scaffold with Interconnected Flow Channels: Co-Cultured Liver Cells and In Vitro Hemocompatibility Assessment." Applied Sciences 11, no. 6 (March 10, 2021): 2473. http://dx.doi.org/10.3390/app11062473.

Full text
Abstract:
The development of large-scale human liver scaffolds equipped with interconnected flow channels in three-dimensional space offers a promising strategy for the advancement of liver tissue engineering. Tissue-engineered scaffold must be blood-compatible to address the demand for clinical transplantable liver tissue. Here, we demonstrate the construction of 3-D macro scaffold with interconnected flow channels using the selective laser sintering (SLS) fabrication method. The accuracy of the printed flow channels was ensured by the incorporation of polyglycolic acid (PGA) microparticles as porogens over the conventional method of NaCl salt leaching. The fabricated scaffold was populated with Hep G2, followed by endothelization with endothelial cells (ECs) grown under perfusion of culture medium for up to 10 days. The EC covered scaffold was perfused with platelet-rich plasma for the assessment of hemocompatibility to examine its antiplatelet adhesion properties. Both Hep G2-covered scaffolds exhibited a markedly different albumin production, glucose metabolism and lactate production when compared to EC-Hep G2-covered scaffold. Most importantly, EC-Hep G2-covered scaffold retained the antiplatelet adhesion property associated with the perfusion of platelet-rich plasma through the construct. These results show the potential of fabricating a 3-D scaffold with interconnected flow channels, enabling the perfusion of whole blood and circumventing the limitation of blood compatibility for engineering transplantable liver tissue.
APA, Harvard, Vancouver, ISO, and other styles
5

Mahanani, Erlina Sih, Anisya Nur Nova Istiyani, and Ratih Sekar Arum. "Effect of Incorporation Platelet Rich Plasma into Synthetic Coral Scaffold toward Epithelial Thickness of Wound Healing." Key Engineering Materials 884 (May 2021): 267–73. http://dx.doi.org/10.4028/www.scientific.net/kem.884.267.

Full text
Abstract:
Tissue re-epithelialization is a process in wound healing. The healing process of wounds needs to pass through a phase of inflammatory, proliferation, and remodeling. Tissue engineering is the technology service in the field of medicine that can be applied in wound healing. A scaffold is a place for the molecule signal and cell growth to form new tissue. This study aims to investigate the ability of synthetic coral scaffold incorporated PRP in wound healing. Rattus norvegicus were used and divided into 4 groups, synthetic coral scaffold incorporated PRP, scaffold only, Spongostan, and control. The wound healing was observed on 3, 7, and 14 days by measuring the epithelial thickness. The average epithelium thickness on day 3 has not formed. The highest epithelial thickness on days 7 and 14 are obtained from the synthetic coral scaffolds incorporated by PRP. The result of this study presented that Synthetic coral scaffold incorporated by PRP has the highest ability for wound healing and increases the process of tissue regeneration.
APA, Harvard, Vancouver, ISO, and other styles
6

Amoo-Achampong, Kelms, Michael K. Krill, Derrick Acheampong, Benedict U. Nwachukwu, and Frank McCormick. "Evaluating strategies and outcomes following rotator cuff tears." Shoulder & Elbow 11, no. 1_suppl (April 26, 2018): 4–18. http://dx.doi.org/10.1177/1758573218768099.

Full text
Abstract:
Introduction Rotator cuff tear surgical repair techniques have significantly progressed. However, tendon retear following primary repair persistently occurs at high rates. Rehabilitation protocols, surgical fixation techniques, biologic therapy with scaffolds, platelet-rich plasma, and even stem cell applications are under study to promote adequate tendon healing. Methods A nonsystematic query of the PubMed database was conducted in July 2016 utilizing the search terms “rotator cuff repair,” “tear,” “rehabilitation,” “scaffold,” “platelet-rich plasma,” and “stem cell” to identify, analyze, and summarize relevant studies. Conclusion Individualized rehabilitation protocols may be the best approach for small to medium sized tears. Surgical fixation will continue to be debated as modifications to single-row technique and increases in suture number have improved tensile strength. Double-row repairs have been associated with higher costs. Transosseous equivalent technique exhibits comparable subjective and objective outcomes to single- and double-row repair at two-year follow-up. Biocompatible scaffold augmentation has showed inconsistent short-term results. Platelet-rich plasma has lacked uniformity in treatment preparation, administration, and outcome measurement with mixed results. Few human studies have suggested decreased retear rates and improved repair maintenance following bone marrow-derived mesenchymal stem cell augmentation. This review reiterated the necessity of additional high-quality, large-sample studies to develop any final verdict regarding efficacy.
APA, Harvard, Vancouver, ISO, and other styles
7

Rosadi, Imam, Karina Karina, Komang A. Wahyuningsih, Iis Rosliana, Tias Widyastuti, Siti Sobariah, Irsyah Afini, and Anggraini Barlian. "KONDROGENESIS ADIPOSE-DERIVED STEM CELLS MENGGUNAKAN PLATELET-RICH PLASMA PADA SCAFFOLD SUTRA." Al-Kauniyah: Jurnal Biologi 13, no. 1 (April 30, 2020): 31–38. http://dx.doi.org/10.15408/kauniyah.v13i1.12053.

Full text
Abstract:
AbstrakStudi tentang kemampuan adipose-derived stem cells (ADSCs) sebagai sel punca yang dapat berdiferensiasi menjadi kondrosit menggunakan platelet-rich plasma (PRP) sebagai subtitusi fetal bovine serum (FBS) telah banyak dilaporkan. Penggunaan medium pertumbuhan dengan kombinasi ADSCs, PRP dan scaffold sutra masih belum banyak dipelajari dalam rekayasa jaringan kartilago. Studi ini bertujuan untuk mempelajari pengaruh medium yang mengandung 5%, 10% dan 20% PRP terhadap proses kondrogenesis ADSCs manusia yang dikultur pada scaffold sutra Bombyx mori Indonesia. Metode penelitian diawali dengan pembuatan scaffold sutra menggunakan metode salt-leaching, isolasi dan kultur ADSCs manusia dari jaringan lemak, uji pertumbuhan ADSCs pada scaffold sutra dengan variasi konsentrasi PRP pada medium serta analisis kadar glikosaminoglikan (GAG). Hasil penelitian menunjukkan bahwa ADSCs yang dikultur menggunakan PRP lebih tinggi laju pertumbuhannya dibandingkan dikultur menggunakan FBS selama 7 hari pengamatan. Kadar GAG yang disekresikan ADSCs kelompok PRP juga lebih tinggi dibandingkan kelompok FBS. Kadar GAG tertinggi pada hari ke-21 pengamatan adalah medium yang mengandung 20% PRP kemudian 10% dan 5%, sedangkan kadar GAG kelompok kontrol cenderung stabil pada kadar yang rendah. Berdasarkan hasil tersebut, medium yang mengandung PRP memiliki potensi dalam menginduksi kondrogenesis ADSCs yang dikultur pada scaffold sutra.Abstract The studies on adipose-derived stem cells (ADSCs) differentiation into chondrocytes using platelet-rich plasma (PRP) as a substitute for fetal bovine serum (FBS) have been reported. However, the combination of ADSCs, PRP and silk fibroin scaffold has not been widely studied for developing cartilage engineering. Therefore, this research aims to study the effect of medium containing 5%, 10% and 20% PRP towards chondrogenesis of human ADSCs cultured on silk fibroin scaffold from Indonesia Bombyx mori. At first, the silk fibroin scaffold was fabricated using a salt-leaching method, then ADSCs were isolated and cultured from adipose tissues. The assays of growth curve and biocompatibility of silk fibroin scaffold toward ADSCs supplemented by PRP as well as glycosaminoglycans (GAG) concentration were conducted later. The results showed that higher absorbance of proliferation rate was on ADSCs supplemented by various PRP concentrations compare to FBS control group for seven days of observation. Level of GAG, which secreted by ADSCs supplemented by a various concentration of PRP, was also higher than the FBS group. The highest level of GAG on day 21 was observed in 20% PRP group then 10% and 5% PRP, while a group of GAG level is stable at low levels. This study concludes that PRP has the potential to induce chondrogenesis ADSCs which cultured on silk fibroin scaffold.
APA, Harvard, Vancouver, ISO, and other styles
8

Utomo, Dwikora Novembri, and Anthoni Yusbida. "CYTOTOXIC EFFECT OF FREEZE DRIED BOVINE CARTILAGE POWDER AND PLATELET RICH PLASMA (PRP) TO MESENCHYMAL STEM CELL (MSCs)." (JOINTS) Journal Orthopaedi and Traumatology Surabaya 6, no. 2 (December 9, 2019): 63. http://dx.doi.org/10.20473/joints.v6i2.2017.63-70.

Full text
Abstract:
Cartilage repair is a challenging clinical problem because the damage is an irreversible condition. Many studies had been performed using several kinds of natural or synthetic scaffold. Attempts to repair articular cartilage using scaffold usually found many problems, lacks the physical structure and mechanical properties necessary to ensure long-term efficacy to cartilage defect. Furthermore, scaffold frequently cause toxicity to the host. Therefore, this study was performed in vitro to test the toxicity effect of scaffold freeze dried bovine cartilage powder and platelets Rich Plasma (PRP). This research was conducted using pure experimental research design in 4 groups of animal stem cells which being added with scaffold freeze dried bovine cartilage scaffold provided with platelet rich plasma. This study using posttest only control group design. The result being processed with MTT assay and spectrophotometer for counting the viable stem cells. There was no significant difference in the amount of macrophage between control and the freeze dried bovine cartilage scaffold provided with PRP (p=0,128). With this result in the number of macrophages between the control with freeze dried bovine cartilage scaffold provided PRP, it can be concluded that these biomaterials have biocompatibility.
APA, Harvard, Vancouver, ISO, and other styles
9

Latifi, Mona, Tahereh Talaei-Khozani, Hossein Mehraban-Jahromi, Mahsa Sani, Mahmood Sadeghi-Atabadi, Abbas Fazel-Anvari, and Maryam Kabir-Salmani. "Fabrication of platelet-rich plasma heparin sulfate/hydroxyapatite/zirconia scaffold." Bioinspired, Biomimetic and Nanobiomaterials 7, no. 2 (June 2018): 122–30. http://dx.doi.org/10.1680/jbibn.17.00038.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Houdek, Matthew T., Cody C. Wyles, Paul G. Stalboerger, Andre Terzic, Atta Behfar, and Steven L. Moran. "Collagen and Fractionated Platelet-Rich Plasma Scaffold for Dermal Regeneration." Plastic and Reconstructive Surgery 137, no. 5 (May 2016): 1498–506. http://dx.doi.org/10.1097/prs.0000000000002094.

Full text
APA, Harvard, Vancouver, ISO, and other styles
11

Ronca, Alfredo, Vincenzo Guarino, Maria Grazia Raucci, Francesca Salamanna, Lucia Martini, Stefania Zeppetelli, Milena Fini, et al. "Large defect-tailored composite scaffolds for in vivo bone regeneration." Journal of Biomaterials Applications 29, no. 5 (June 20, 2014): 715–27. http://dx.doi.org/10.1177/0885328214539823.

Full text
Abstract:
The discovery of new strategies to repair large segmental bone defects is currently an open challenge for worldwide clinicians. In the treatment of critical-sized bone defects, an alternative strategy to traditional bone grafting is always more frequently the use of tailor-made scaffolds modelled on the final size and shape of the implant site. Here, poly-ε-caprolactone-based composite scaffolds including poly-l-lactic acid continuous fibres and hyaluronan derivates (i.e. HYAFF11®) have been investigated for the peculiar 3D architecture characterized by interconnected macroporous networks and tunable mechanical properties. Composite scaffolds were immersed in simulated body fluid solution in order to support in vivo tissue in-growth. Scaffolds loaded with autologous cells (bone marrow stromal cells) plus platelet-rich plasma and osteoconductive protein such bone morphogenetic protein-7 were also tested to evaluate eventual enhancement in bone regeneration. The morphological and mechanical properties of poly-l-lactic acid-reinforced composite scaffolds have been studied to identify the optimal scaffold design to match the implant-site requirements of sheep metatarsal defects. Dynamic mechanical tests allowed to underline the viscoelastic response of the scaffold – resulting in elastic moduli from 2.5 to 1.3 MPa, suitable to temporarily support the structural function of damaged bone tissue. In vivo preliminary investigations in a sheep model of metatarsus shaft defect also showed the attitude of the scaffold to promote osteogenesis, preferentially in association with bone marrow stromal cell and platelet-rich plasma, even if the highest amount of mature bone was reached in the case of scaffold loaded with human bone morphogenetic protein-7 released via hydrolytic degradation of HYAFF11® phases in the implant site.
APA, Harvard, Vancouver, ISO, and other styles
12

Rohman, Géraldine, Credson Langueh, Salah Ramtani, Jean-Jacques Lataillade, Didier Lutomski, Karim Senni, and Sylvie Changotade. "The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering." Polymers 11, no. 6 (June 9, 2019): 1016. http://dx.doi.org/10.3390/polym11061016.

Full text
Abstract:
Due to their elastomeric behavior, polyurethane-based scaffolds can find various applications in soft-tissue engineering. However, their relatively inert surface has to be modified in order to improve cell colonization and control cell fate. The present study focuses on porous biodegradable scaffolds based on poly(ester-urea-urethane), functionalized concomitantly to the scaffold elaboration with low-molecular-weight (LMW) fucoidan; and their bio-activation with platelet rich plasma (PRP) formulations with the aim to promote cell response. The LMW fucoidan-functionalization was obtained in a very homogeneous way, and was stable after the scaffold sterilization and incubation in phosphate-buffered saline. Biomolecules from PRP readily penetrated into the functionalized scaffold, leading to a biological frame on the pore walls. Preliminary in vitro assays were assessed to demonstrate the improvement of scaffold behavior towards cell response. The scaffold bio-activation drastically improved cell migration. Moreover, cells interacted with all pore sides into the bio-activated scaffold forming cell bridges across pores. Our work brought out an easy and versatile way of developing functionalized and bio-activated elastomeric poly(ester-urea-urethane) scaffolds with a better cell response.
APA, Harvard, Vancouver, ISO, and other styles
13

Wang, Ping, Yili Qu, and Yi Man. "Platelet-rich plasma as a scaffold for injectable soft-tissue augmentation." Cytotherapy 12, no. 5 (September 2010): 701–2. http://dx.doi.org/10.3109/14653249.2010.487901.

Full text
APA, Harvard, Vancouver, ISO, and other styles
14

Li, Guangjun, Wen Shen, Xing Tang, Guowei Mo, Liqin Yao, and Jixing Wang. "Combined use of calcium phosphate cement, mesenchymal stem cells and platelet-rich plasma for bone regeneration in critical-size defect of the femoral condyle in mini-pigs." Regenerative Medicine 16, no. 5 (May 2021): 451–64. http://dx.doi.org/10.2217/rme-2020-0099.

Full text
Abstract:
Aim: To investigate the outcome of autologous bone marrow mesenchymal stem cells (BMMSCs) and platelet-rich plasma in combination with calcium phosphate cement (CPC) scaffold to reconstruct femoral critical bone defects in mini-pigs. Materials & methods: Scanning electron microscopy, micro-computed tomography evaluation and quantitative histological assessment were used. Results & conclusion: BMMSCs were attached to the CPC scaffold after 7 days of culture and decreased the residual CPC material in each group at 12 weeks compared with 6 weeks. The newly formed bone area was higher in the CPC+SC+P group than in the CPC group at each time point (all p < 0.05). The strategy of CPC combined with BMMSCs and platelet-rich plasma might be an effective method to repair bone defects.
APA, Harvard, Vancouver, ISO, and other styles
15

Mariani, Erminia, and Lia Pulsatelli. "Platelet Concentrates in Musculoskeletal Medicine." International Journal of Molecular Sciences 21, no. 4 (February 16, 2020): 1328. http://dx.doi.org/10.3390/ijms21041328.

Full text
Abstract:
Platelet concentrates (PCs), mostly represented by platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are autologous biological blood-derived products that may combine plasma/platelet-derived bioactive components, together with fibrin-forming protein able to create a natural three-dimensional scaffold. These types of products are safely used in clinical applications due to the autologous-derived source and the minimally invasive application procedure. In this narrative review, we focus on three main topics concerning the use of platelet concentrate for treating musculoskeletal conditions: (a) the different procedures to prepare PCs, (b) the composition of PCs that is related to the type of methodological procedure adopted and (c) the clinical application in musculoskeletal medicine, efficacy and main limits of the different studies.
APA, Harvard, Vancouver, ISO, and other styles
16

Bezgin, Tugba, Ayca Dilara Yilmaz, Burcu Nihan Celik, Mehmet Eray Kolsuz, and Hayriye Sonmez. "Efficacy of Platelet-rich Plasma as a Scaffold in Regenerative Endodontic Treatment." Journal of Endodontics 41, no. 1 (January 2015): 36–44. http://dx.doi.org/10.1016/j.joen.2014.10.004.

Full text
APA, Harvard, Vancouver, ISO, and other styles
17

Ghaffarinovin, Zeinab, Omid Soltaninia, Yousef Mortazavi, Abdolreza Esmaeilzadeh, and Samad Nadri. "Repair of rat cranial bone defect by using amniotic fluid-derived mesenchymal stem cells in polycaprolactone fibrous scaffolds and platelet-rich plasma." BioImpacts 11, no. 3 (July 8, 2020): 209–17. http://dx.doi.org/10.34172/bi.2021.28.

Full text
Abstract:
Introduction: Tissue regenerative medicine strategies, as a promising alternative has become of major interest to the reconstruction of critical size bone defects. This study evaluated the effects of the simultaneous application of polycaprolactone (PCL), amniotic fluid mesenchymal stem cells (AF-MSCs) and platelet-rich plasma (PRP) on the repair of rat cranial bone defects. Methods: The AF-MSCs were isolated at the end of the second week of pregnancy in rats. PRP obtained from rat blood and the random PCL fibrous scaffolds were prepared using the electrospinning method. Circular full thickness (5 mm) bone defects were developed on both sides of the parietal bones (animal number=24) and the scaffolds containing AF-MSCs and PRP were implanted in the right lesions. Thereafter, after eight weeks the histological and immunohistochemistry studies were performed to evaluate the bone formation and collagen type I expression. Results: The spindle-shaped mesenchymal stem cells were isolated and the electron microscope images indicated the preparation of a random PCL scaffold. Immunohistochemical findings showed that collagen type I was expressed by AF-MSCs cultured on the scaffold. The results of hematoxylin and eosin (H & E) staining indicated the formation of blood vessels in the presence of PRP. Additionally, immunofluorescence findings suggested that PRP had a positive effect on collagen type I expression. Conclusion: The simultaneous application of fibrous scaffold + AF-MSCs + PRP has positive effects on bone regeneration. This study showed that PRP can affect the formation of new blood vessels in the scaffold transplanted in the bone defect.
APA, Harvard, Vancouver, ISO, and other styles
18

Zhang, Liang, Hongquan Qiu, Dongzhi Wang, Haiyan Miao, Yi Zhu, Qingsong Guo, Yibing Guo, and Zhiwei Wang. "Enhanced vascularization and biocompatibility of rat pancreatic decellularized scaffolds loaded with platelet-rich plasma." Journal of Biomaterials Applications 35, no. 3 (June 20, 2020): 313–30. http://dx.doi.org/10.1177/0885328220933890.

Full text
Abstract:
The ultimate goal of pancreatic tissue engineering is to create a long-lived substitute organ to treat diabetes. However, the lack of neovascularization and the occurrence of immune response limit the efficacy of tissue-engineered pancreas after in vivo transplantation. Platelet-rich plasma (PRP) is an autologous platelet concentrate containing a large number of growth factors and immunoregulatory factors. The aim of this study was to evaluate rat pancreatic decellularized scaffold (PDS) loaded with PRP for vascularization, host inflammatory response and macrophage polarization in an animal model. The study results indicated that compared to PDS, PRP-loading PDS exhibited the enhanced mechanical properties and released growth factors in a slow and sustained manner to supplement the loss of growth factors during decellularization. In vitro, human umbilical vein endothelial cells (HUVECs) were seeded in PDS and PRP-loading PDS, and cultured in the circular perfusion system. When compared with PDS, PRP-loading PDS significantly promoted the colonization, proliferation and pro-angiogenic genes expression of cells on scaffolds. In vivo, PDS loaded with PRP then re-endothelialized with HUVECs were implanted subcutaneously in rats, which enhanced the angiogenesis of scaffolds, inhibited the host inflammatory response, and induced the polarization dominated by pro-regenerative M2 macrophages that also facilitated tissue vascular regeneration. Thus, the re-endothelialized PRP-loading PDS may represent a promising bioengineered pancreas with sustained vascularization and excellent biocompatibility.
APA, Harvard, Vancouver, ISO, and other styles
19

Caruana, Andrea, Daniele Savina, José Paulo Macedo, and Sandra Clara Soares. "From Platelet-Rich Plasma to Advanced Platelet-Rich Fibrin: Biological Achievements and Clinical Advances in Modern Surgery." European Journal of Dentistry 13, no. 02 (May 2019): 280–86. http://dx.doi.org/10.1055/s-0039-1696585.

Full text
Abstract:
AbstractIn the past 20 years, the platelet concentrates have evolved from first-generation products, i.e., platelet-rich plasma (PRP) and plasma rich in growth factors to the second-generation products such as leukocyte-platelet-rich fibrin (L-PRF) and advanced platelet-rich fibrin (A-PRF). These autologous products with a higher leukocyte inclusion and flexible fibrin mesh act as a scaffold to increase cellular migration in the angiogenic, osteogenic, and antimicrobial potential of these biomaterials in tissue regeneration. In the second-generation platelet concentrates, the protocols are easier, cheaper, and faster with an entire physiological fibrin matrix, resulting in a tridimensional mesh, not as rigid as one of the first generations. This allows the slow release of molecules over a longer period of time and triggers the healing and regenerative process at the site of injury. The potential of A-PRF to mimic the physiology and immunology of wound healing is also due to the high concentration of growth factors released as follows: vascular endothelial growth factor, platelet-derived growth factor, transforming growth factor-β, and anti-inflammatory cytokines that stimulate tissue cicatrization, vessels formation, and bone cell proliferation and differentiation. Furthermore, the number of neutrophils and monocytes/macrophages is higher releasing important chemotactic molecules such as chemokine ligand-5 and eotaxin. Thus, L-PRF and A-PRF have been used, especially in implantology, periodontology, and maxillofacial surgery. Future clinical applications include tissue regeneration/grafts, ulcers/skin necrosis in the diabetic patient and others, plastic surgery, and even musculoskeletal lesions.
APA, Harvard, Vancouver, ISO, and other styles
20

Nithyanandham Masilamani and Dhanraj Ganapathy. "Awareness about Regenerative Therapy with of Platelet Rich Plasma (PRP) among Dental Students." International Journal of Research in Pharmaceutical Sciences 11, SPL3 (September 19, 2020): 962–66. http://dx.doi.org/10.26452/ijrps.v11ispl3.3058.

Full text
Abstract:
Platelet Rich Plasma(PRP), serving as a vehicle and wellspring of development factors, is a homologous plasma formulation with congregated platelets broadly studied for its applications as a scaffold in cell-based intervention and tissue engineering. The aim of the study was to assess the awareness about regenerative therapy with platelet Rich Plasma (PRP) among dental students. A cross-sectional study was done with a self-administered questionnaire with ten questions circulated among 100 dental students. The questionnaire assessed the awareness about PRP regenerative therapy in surgical applications, their uses, periodontal applications, surgical applications and bone healing. The responses were recorded and . 82% of the respondents were aware of surgical applications of PRP regenerative therapy. 73% were aware of uses of regenerative therapy. 77% of the respondents were aware of periodontal applications of PRP regenerative therapy. 75% of the respondents were aware of surgical implant applications of PRP regenerative therapy. 78 % of the respondents were aware of bone healing properties of PRP regenerative therapy. The awareness about the use of PRP regenerative therapy in dentalapplications is high among dental students. Increased awareness programs and sensitization and continuing dental education programs along with more significant importance to the curricular modifications, can further enhance knowledge and awareness about PRP regenerative therapy.
APA, Harvard, Vancouver, ISO, and other styles
21

Shi, Zhongmin. "Management of Hepple Stage V Osteochondral Lesion of Talus with Platelet-Rich Plasma (PRP) Scaffold." Foot & Ankle Orthopaedics 2, no. 3 (September 1, 2017): 2473011417S0003. http://dx.doi.org/10.1177/2473011417s000367.

Full text
Abstract:
Category: Ankle Introduction/Purpose: There is no consensus on treatment or prognosis for Hepple stage V Osteochondral lesion of talus (OLTs), especially for lesion more than 1.5 cm2. This research was to investigate surgical techniques and clinical outcomes of platelet-rich plasma (PRP) scaffold for Hepple stage V OLTs. Methods: 14 patients were treated by cancellous bone graft with PRP gel scaffold between 2013 and 2015 with average age of 38.9 years old and mean set of 23.5 months. Ankle X-ray and MRI were obtained at the final follow-up for evaluation. Functional outcomes were scored by the Visual Analog Scale (VAS) score, American Orthopaedics Foot and Ankle Society (AOFAS) ankle- hindfoot score and Short Form (36) Survey score (SF-36). Range of motion (ROM) of ankle joint and complications were also recorded. Results: 13 patients got the final follow-up with a mean duration of 18 months. MRI showed complete regeneration of subchondral bone and cartilage in all patients. The post-operative VAS, AOFAS ankle-hindfoot score and SF-36 score improved significantly (P<0.0001) without obvious complications. Conclusion: We suggest that for the patients of Hepple stage V OLTs, cancellous bone graft with PRP scaffold may be a safe and effective treatment.
APA, Harvard, Vancouver, ISO, and other styles
22

Nadra, Meral, Wanting Niu, Motoichi Kurisawa, Dominique Rousson, and Myron Spector. "Platelet-Rich Plasma Lysate-Incorporating Gelatin Hydrogel as a Scaffold for Bone Reconstruction." Bioengineering 9, no. 10 (September 29, 2022): 513. http://dx.doi.org/10.3390/bioengineering9100513.

Full text
Abstract:
In implant dentistry, large vertical and horizontal alveolar ridge deficiencies in mandibular and maxillary bone are challenges that clinicians continue to face. One of the limitations of porous blocks for reconstruction of bone in large defects in the oral cavity, and in the musculoskeletal system, is that fibrin clot does not adequately fill the interior pores and does not persist long enough to accommodate cell migration into the center of the block. The objective of our work was to develop a gelatin-based gel incorporating platelet-rich plasma (PRP) lysate, to mimic the role that a blood clot would normally play to attract and accommodate the migration of host osteoprogenitor and endothelial cells into the scaffold, thereby facilitating bone reconstruction. A conjugate of gelatin (Gtn) and hydroxyphenyl propionic acid (HPA), an amino-acid-like molecule, was commended for this application because of its ability to undergo enzyme-mediated covalent cross-linking to form a hydrogel in vivo, after being injected as a liquid. The initiation and propagation of cross-linking were under the control of horseradish peroxidase and hydrogen peroxide, respectively. The objectives of this in vitro study were directed toward evaluating: (1) the migration of rat mesenchymal stem cells (MSCs) into Gtn–HPA gel under the influence of rat PRP lysate or recombinant platelet-derived growth factor (PDGF)-BB incorporated into the gel; (2) the differentiation of MSCs, incorporated into the gel, into osteogenic cells under the influence of PRP lysate and PDGF-BB; and (3) the release kinetics of PDGF-BB from gels incorporating two formulations of PRP lysate and recombinant PDGF-BB. Results: The number of MSCs migrating into the hydrogel was significantly (3-fold) higher in the hydrogel group incorporating PRP lysate compared to the PDGF-BB and the blank gel control groups. For the differentiation/osteogenesis assay, the osteocalcin-positive cell area percentage was significantly higher in both the gel/PRP and gel/PDGF-BB groups, compared to the two control groups: cells in the blank gels grown in cell expansion medium and in osteogenic medium. Results of the ELISA release assay indicated that Gtn–HPA acted as an effective delivery vehicle for the sustained release of PDGF-BB from two different PRP lysate batches, with about 60% of the original PDGF-BB amount in the two groups remaining in the gel at 28 days. Conclusions: Gtn–HPA accommodates MSC migration. PRP-lysate-incorporating hydrogels chemoattract increased MSC migration into the Gtn–HPA compared to the blank gel. PRP-lysate- and the PDGF-BB-incorporating gels stimulate osteogenic differentiation of the MSCs. The release of the growth factors from Gtn–HPA containing PRP lysate can extend over the period of time (weeks) necessary for bone reconstruction. The findings demonstrate that Gtn–HPA can serve as both a scaffold for cell migration and a delivery vehicle that allows sustained and controlled release of the incorporated therapeutic agent over extended periods of time. These findings commend Gtn–HPA incorporating PRP lysate for infusion into porous calcium phosphate blocks for vertical and horizontal ridge reconstruction, and for other musculoskeletal applications.
APA, Harvard, Vancouver, ISO, and other styles
23

Vinod, Elizabeth, Deepak Vinod Francis, Soosai Manickam Amirtham, Solomon Sathishkumar, and P. R. J. V. C. Boopalan. "Allogeneic platelet rich plasma serves as a scaffold for articular cartilage derived chondroprogenitors." Tissue and Cell 56 (February 2019): 107–13. http://dx.doi.org/10.1016/j.tice.2018.12.006.

Full text
APA, Harvard, Vancouver, ISO, and other styles
24

Abbassy, Hadeer A., Laila M. Montaser, and Sherin M. Fawzy. "Articular cartilage tissue engineering with stem cells implanted onto nanoscaffolds and platelet-rich plasma." International Journal of Research in Orthopaedics 3, no. 3 (April 25, 2017): 322. http://dx.doi.org/10.18203/issn.2455-4510.intjresorthop20170985.

Full text
Abstract:
<p class="abstract">Musculoskeletal medicine targets both cartilage regeneration and healing of soft tissues. Articular cartilage repair and regeneration is primarily considered to be due to its poor regenerative properties. Cartilage defects due to joint injury, aging, or osteoarthritis have low self-repair ability thus they are most often irreversible as well as being a major cause of joint pain and chronic disability. Unfortunately, current methods do not seamlessly restore hyaline cartilage and may lead to the formation of fibro- or continue hypertrophic cartilage. Deficiency of efficient modalities of therapy has invited research to combine stem cells, scaffold materials and environmental factors through tissue engineering. Articular cartilage tissue engineering aims to repair, regenerate, and hence improve the function of injured or diseased cartilage. This holds great potential and has evoked intense interest in improving cartilage therapy. Platelet-rich plasma (PRP) and/or stem cells may be influential for tissue repair as well as cartilage regenerative processes. A great promise to advance current cartilage therapies toward achieving a consistently successful modality has been held for addressing cartilage afflictions. The use of stem cells, novel biologically inspired scaffolds and, emerging nanotechnology may be the best way to reach this objective via tissue engineering. A current and emergent approach in the field of cartilage tissue engineering is explained in this review for specific application. In the future, the development of new strategies using stem cells seeded in scaffolds and the culture medium supplemented with growth factors could improve the quality of the newly formed cartilage<span lang="EN-IN">.</span></p>
APA, Harvard, Vancouver, ISO, and other styles
25

Liu, Jiawei, Fei Lu, HongLei Chen, Rong Bao, Zhiquan Li, Bitao Lu, Kun Yu, Fangying Dai, Dayang Wu, and Guangqian Lan. "Healing of skin wounds using a new cocoon scaffold loaded with platelet-rich or platelet-poor plasma." RSC Advances 7, no. 11 (2017): 6474–85. http://dx.doi.org/10.1039/c6ra27021b.

Full text
APA, Harvard, Vancouver, ISO, and other styles
26

Narayanaswamy, Ragunanthan, Bishnu Prasad Patro, Naveen Jeyaraman, Prakash Gangadaran, Ramya Lakshmi Rajendran, Arulkumar Nallakumarasamy, Madhan Jeyaraman, Prasanna Ramani, and Byeong-Cheol Ahn. "Evolution and Clinical Advances of Platelet-Rich Fibrin in Musculoskeletal Regeneration." Bioengineering 10, no. 1 (January 3, 2023): 58. http://dx.doi.org/10.3390/bioengineering10010058.

Full text
Abstract:
Over the past few decades, various forms of platelet concentrates have evolved with significant clinical utility. The newer generation products, including leukocyte-platelet-rich fibrin (L-PRF) and advanced platelet-rich fibrin (A-PRF), have shown superior biological properties in musculoskeletal regeneration than the first-generation concentrates, such as platelet-rich plasma (PRP) and plasma rich in growth factors. These newer platelet concentrates have a complete matrix of physiological fibrin that acts as a scaffold with a three-dimensional (3D) architecture. Further, it facilitates intercellular signaling and migration, thereby promoting angiogenic, chondrogenic, and osteogenic activities. A-PRF with higher leukocyte inclusion possesses antimicrobial activity than the first generations. Due to the presence of enormous amounts of growth factors and anti-inflammatory cytokines that are released, A-PRF has the potential to replicate the various physiological and immunological factors of wound healing. In addition, there are more neutrophils, monocytes, and macrophages, all of which secrete essential chemotactic molecules. As a result, both L-PRF and A-PRF are used in the management of musculoskeletal conditions, such as chondral injuries, tendinopathies, tissue regeneration, and other sports-related injuries. In addition to this, its applications have been expanded to include the fields of reconstructive cosmetic surgery, wound healing in diabetic patients, and maxillofacial surgeries.
APA, Harvard, Vancouver, ISO, and other styles
27

Shi, Zhongmin. "Management of Hepple Stage V Osteochondral Lesion of the Talus with a Platelet-Rich Plasma Scaffold." Foot & Ankle Orthopaedics 3, no. 3 (July 1, 2018): 2473011418S0043. http://dx.doi.org/10.1177/2473011418s00438.

Full text
Abstract:
Category: Ankle Introduction/Purpose: There has been no consensus on the treatment or prognosis of Hepple stage V osteochondral lesions of the talus (OLTs), especially for lesions greater than 1.5 cm2 in size. The objective of this study was to investigate the clinical outcomes achieved upon application of a platelet-rich plasma (PRP) scaffold with a cancellous bone autograft for Hepple stage V OLTs. Methods: Fourteen patients (mean age, 39 years) were treated with a cancellous bone graft and a PRP scaffold between 2013 and 2015. The mean time to surgical treatment was 23.5 months. Ankle X-ray and magnetic resonance imaging were performed at the final follow-up. Functional outcomes were evaluated according to the Visual Analog Scale (VAS) score, American Orthopaedic Foot and Ankle Society (AOFAS) score, and Short Form36 (SF-36) score.The range of motion (ROM) of the ankle joint and complications also were recorded. Results: Thirteen patients completed the full follow-up, with a mean follow-up duration of 18 months. MRI demonstrated the complete regeneration of subchondral bone and cartilage in all patients.The postoperative VAS, AOFAS ankle and hindfoot, and SF-36 scores were improved significantly (all 𝑃 < 0.001) without obvious complications. Conclusion: We suggest that, for the Hepple stage V OLTs, management with cancellous bone graft and PRP scaffold may be a safe and effective treatment.
APA, Harvard, Vancouver, ISO, and other styles
28

Gu, Wenqi, Tanzhu Li, Zhongmin Shi, Guohua Mei, Jianfeng Xue, Jian Zou, Xiaokang Wang, Haotong Zhang, and Hongwei Xu. "Management of Hepple Stage V Osteochondral Lesion of the Talus with a Platelet-Rich Plasma Scaffold." BioMed Research International 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/6525373.

Full text
Abstract:
There has been no consensus on the treatment or prognosis of Hepple stage V osteochondral lesions of the talus (OLTs), especially for lesions greater than 1.5 cm2in size. The objective of this study was to investigate the clinical outcomes achieved upon application of a platelet-rich plasma (PRP) scaffold with a cancellous bone autograft for Hepple stage V OLTs. Fourteen patients (mean age, 39 years) were treated with a cancellous bone graft and a PRP scaffold between 2013 and 2015. The mean time to surgical treatment was 23.5 months. Ankle X-ray and magnetic resonance imaging were performed at the final follow-up. Functional outcomes were evaluated according to the Visual Analog Scale (VAS) score, American Orthopaedic Foot and Ankle Society (AOFAS) score, and Short Form 36 (SF-36) score. The range of motion (ROM) of the ankle joint and complications also were recorded. Thirteen patients completed the full follow-up, with a mean follow-up duration of 18 months. MRI demonstrated the complete regeneration of subchondral bone and cartilage in all patients. The postoperative VAS, AOFAS ankle and hindfoot, and SF-36 scores were improved significantly (allP<0.001) without obvious complications. We suggest that, for the Hepple stage V OLTs, management with cancellous bone graft and PRP scaffold may be a safe and effective treatment.
APA, Harvard, Vancouver, ISO, and other styles
29

Carvalho, Renata Aquino de, Valmir Vieira Rocha Júnior, Antonio José Felix Carvalho, Heloisa Sobreiro Selistre de Araújo, Mônica Rosas Costa Iemma, Eliane Trovatti, and André Capaldo Amaral. "Poly-(lactic acid) and fibrin bioactive cellularized scaffold for use in bone regenerative medicine: Proof of concept." Journal of Bioactive and Compatible Polymers 36, no. 3 (March 5, 2021): 171–84. http://dx.doi.org/10.1177/0883911521996402.

Full text
Abstract:
Bone regenerative medicine (BRM) aims to overcome the limitations of conventional treatments for critical bone defects by developing therapeutic strategies, based on temporary bioactive substitutes, capable of stimulating, sustaining, and guiding tissue regeneration. The aim of this study was to validate the “proof of concept” of a cellularized bioactive scaffold and establish its potential for use in BRM. For this purpose, three-dimensional scaffolds of poly-(lactic acid) (PLA), produced by the additive manufacturing technique, were incorporated into a human platelet-rich plasma (PRP-h) fibrin matrix containing human infrapatellar fat pad mesenchymal stem cells (hIFPMSC). The scaffolds (PLA/finbrin-bioactive) were kept under ideal culture conditions in a medium free from fetal bovine serum and analyzed at 5 and 10 days by Scanning Electron Microscopy (SEM), Fourrier Transform Infrared (FTIR), Circular Dichroism and fluorescence microscopy. The results demonstrated the feasibility of obtaining a rigid, cytocompatible, and cellularized three-dimensional structure. In addition, PRP platelets and leukocytes were able to provide a bioactive environment capable of maintaining the viability of hIFPMSC into scaffolds. The results validate the concept of a customizable, bioactive, cellularized, and non-immunogenic strategy for application in BRM.
APA, Harvard, Vancouver, ISO, and other styles
30

Park, Sung-Hee, Hantai Kim, Yun Yeong Lee, Yeon Ju Kim, Jeong Hun Jang, Oak-Sung Choo, and Yun-Hoon Choung. "Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media Surgeries." Polymers 14, no. 5 (February 23, 2022): 877. http://dx.doi.org/10.3390/polym14050877.

Full text
Abstract:
Mastoidectomy is a surgical procedure for the treatment of chronic otitis media. This study investigated the ability of rat stromal vascular fraction cells (rSVF) in combination with polycaprolactone (PCL) scaffolds and osteogenic differentiation-enhancing blood products to promote the regeneration of mastoid bone defect. Twenty male Sprague Dawley rats were randomly divided according to obliteration materials: (1) control, (2) PCL scaffold only, (3) rSVFs + PCL, (4) rSVFs + PCL + platelet-rich plasma, and (5) rSVFs + PCL + whole plasma (WP). At 7 months after transplantation, the rSVFs + PCL + WP group showed remarkable new bone formation in the mastoid. These results indicate that SVFs, PCL scaffolds, and blood products accelerate bone regeneration for mastoid reconstruction. Autologous SVF cells with PCL scaffolds and autologous blood products are promising composites for mastoid reconstruction which can be easily harvested after mastoidectomy. With this approach, the reconstruction of mastoid bone defects can be performed right after mastoidectomy as a one-step procedure which can offer efficiency in the clinical field.
APA, Harvard, Vancouver, ISO, and other styles
31

Maffulli, Nicola, Umile Giuseppe Longo, Mattia Loppini, Alessandra Berton, Filippo Spiezia, and Vincenzo Denaro. "Tissue Engineering for Rotator Cuff Repair: An Evidence-Based Systematic Review." Stem Cells International 2012 (2012): 1–11. http://dx.doi.org/10.1155/2012/418086.

Full text
Abstract:
The purpose of this systematic review was to address the treatment of rotator cuff tears by applying tissue engineering approaches to improve tendon healing, specifically platelet rich plasma (PRP) augmentation, stem cells, and scaffolds. Our systematic search was performed using the combination of the following terms: “rotator cuff”, “shoulder”, “PRP”, “platelet rich plasma”, “stemcells”, “scaffold”, “growth factors”, and “tissue engineering”. No level I or II studies were found on the use of scaffolds and stem cells for rotator cuff repair. Three studies compared rotator cuff repair with or without PRP augmentation. All authors performed arthroscopic rotator cuff repair with different techniques of suture anchor fixation and different PRP augmentation. The three studies found no difference in clinical rating scales and functional outcomes between PRP and control groups. Only one study showed clinical statistically significant difference between the two groups at the 3-month followup. Any statistically significant difference in the rates of tendon rerupture between the control group and the PRP group was found using the magnetic resonance imaging. The current literature on tissue engineering application for rotator cuff repair is scanty. Comparative studies included in this review suggest that PRP augmented repair of a rotator cuff does not yield improved functional and clinical outcome compared with non-augmented repair at a medium and long-term followup.
APA, Harvard, Vancouver, ISO, and other styles
32

Ferraraz, Débora Carajiliascov, Nelson Hidekazu Tatsui, Leonardo Ribeiro Rodrigues, and Christiane Bertachini Lombello. "Platelet-rich plasma as supplement and scaffold for the culture of Vero cell line." Research on Biomedical Engineering 35, no. 1 (February 18, 2019): 1–9. http://dx.doi.org/10.1007/s42600-019-00001-6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
33

ZHONG, DA, CHENG-GONG WANG, KE YIN, QIANDE LIAO, XING ZHOU, AN-SONG LIU, and LING-YU KONG. "In vivo ossification of a scaffold combining β-tricalcium phosphate and platelet-rich plasma." Experimental and Therapeutic Medicine 8, no. 5 (September 15, 2014): 1381–88. http://dx.doi.org/10.3892/etm.2014.1969.

Full text
APA, Harvard, Vancouver, ISO, and other styles
34

Kwak, Hong Suk, Jinwoo Nam, Ji-hye Lee, Hee Joong Kim, and Jeong Joon Yoo. "Meniscal repairin vivousing human chondrocyte-seeded PLGA mesh scaffold pretreated with platelet-rich plasma." Journal of Tissue Engineering and Regenerative Medicine 11, no. 2 (June 19, 2014): 471–80. http://dx.doi.org/10.1002/term.1938.

Full text
APA, Harvard, Vancouver, ISO, and other styles
35

Amsar, Rizka Musdalifah, Anggraini Barlian, Hermawan Judawisastra, Untung Ari Wibowo, and Karina Karina. "Cell penetration and chondrogenic differentiation of human adipose derived stem cells on 3D scaffold." Future Science OA 7, no. 8 (September 2021): FSO734. http://dx.doi.org/10.2144/fsoa-2021-0040.

Full text
Abstract:
The ability of cells to penetrate the scaffold and differentiate into chondrocyte is important in cartilage engineering. The aim of this research was to evaluate the use of silk fibroin 3D scaffold in facilitating the growth of stem cell and to study the role of L-ascorbic acid and platelet rich plasma (PRP) in proliferation and differentiation genes. Cell penetration and type II collagen content in the silk fibroin scaffold was analyzed by confocal microscopy. Relative expressions of CDH2, CCND1, CTNNB1 and COL2A1 were analyzed by reverse transcription-quantitative PCR (RT-qPCR). The silk fibroin 3D scaffold could facilitate cell penetration. L-ascorbic acid and PRP increased the expression of CDH2 and COL2A1 on the 21st day of treatment while PRP inhibited CTNNB1 and CCND1.
APA, Harvard, Vancouver, ISO, and other styles
36

Mahanani, Erlina Sih, Indra Bachtiar, and Ika Dewi Ana. "Human Mesenchymal Stem Cells Behavior on Synthetic Coral Scaffold." Key Engineering Materials 696 (May 2016): 205–11. http://dx.doi.org/10.4028/www.scientific.net/kem.696.205.

Full text
Abstract:
For bone tissue engineering, corals have long history to be used as scaffold to promote bone regeneration. However the use of a lot of corals may damage their habitates. For this reason, a strategy to mimic coral in a synthetic form is needed. As an ideal scaffold, synthetic coral must provide structure and initial support for cell attachment and proliferation. The aim of this study was to investigate the attachment and proliferation of human Mesenchymal Stem Cells (h-MSC) on synthetic coral scaffold, to provide information on the behavior of h-MSC on the designated scaffold. Synthetic coral scaffolds were prepared from bovine gelatine and CaCO3 with 5:5 in 10% w/v concentration in aquadest. Sodium citrate was used as dispersant in the suspension. Gelatin-CaCO3 suspension was moulded in a plastic cover of 24 well plate, then freezed at -20°C for 24 hours, freeze dried for 24 hours and continued by dehydrothermal crosslinking for 72 hours. After the fabrication, synthetic coral scaffolds were subjects to cover the bottom of the well for cell culture. Human Mesenchymal Stem Cells (h-MSC) were seeded and divided into 2 groups, control group without scaffold and the one with scaffold. All groups were incubated for 3, 6, and 24 hours. Cells attatchment were determined by deduction of the cells unattached from total cells seeding. Proliferation of h-MSC were done in 3 groups ie., control group without scaffold, scaffold only and scaffold incorporated Platelet Rich Plasma (PRP) in the bottom of well. All groups were incubated for 24, 48 and 72 hours. Human Mesenchymal Stem Cells attached faster to synthetic coral scaffold than the control. Its proliferation behavior was faster in the scaffold incorporated PRP, showing better interaction of scaffold and cells with the incorporation of morphogenetic factor.
APA, Harvard, Vancouver, ISO, and other styles
37

Chen, Wei-Hong, Hen-Yu Liu, Ching-Yu Tsai, Chia-che Wu, Hong-Jian Wei, Alice Liu, Ming-Tang Lai, Chiung-Fang Huang, and Win-Ping Deng. "The Potential Use of Platelet-Rich Plasma to Reconstruct the Microtia Chondrocyte in Human Auricular Cartilage Regeneration." Journal of Nanomaterials 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/250615.

Full text
Abstract:
Microtia is characterized as an incomplete auricular development and surgical reconstruction for microtia is still limited even with emerging developments. This study aimed to apply bionanomaterials (PRP/collagen scaffold) for human auricular neocartilage reconstruction by using microtia chondrocytes. The results showed that PRP (TGF-β1 750 pg/mL and 1 ng/mL) increased cell viability of microtia chondrocytes during in vitro 9-day cultures. Additionally, chondrogenic-specific mRNA of Aggrecan and type II collagen (Col II) was significantly and continuously expressed with PRP treatment during the 21-day in vitro expansion. Tissue engineering of auricular neocartilage was performed by seeding microtia chondrocytes in bionanomaterials (PRP/collagen scaffold) 3-dimensional (3D) cultures. Immunohistochemistry (IHC) of Col II showed intensive signals between cells and matrix after 4-week cultures. Conclusion. Our results demonstrated that PRP promotes proliferation and redifferentiation of microtia chondrocytes and provides regenerative potentials in auricular neocartilage reconstruction.
APA, Harvard, Vancouver, ISO, and other styles
38

DEMARCO, Giulia Tarquinio, Laura Borges KIRSCHNICK, Luis Bayardo WATSON, Marcus Cristian MUNIZ CONDE, Flávio Fernando DEMARCO, and Luiz Alexandre CHISINI. "What is the clinical applicability of regenerative therapies in dentistry?" RGO - Revista Gaúcha de Odontologia 65, no. 4 (December 2017): 359–67. http://dx.doi.org/10.1590/1981-863720170002000113112.

Full text
Abstract:
ABSTRACT Regenerative therapies have been widely developed in dentistry and it is important to incorporate dentists’ knowledge of these new therapies into the dental clinic routine. This study reviewed the literature on regenerative therapies and clinical applications. Tissue engineering has contributed to changes in the paradigm of restorative health sciences. Its pillars underpin the techniques of tissue and organ regeneration. Despite the majority of studies in this field being in vitro, a range of preclinical studies and methodologies has been formed using these principles and they are already being used on humans. The use of platelet-rich plasma and platelet-rich fibrin in surgery as natural scaffolds for the reestablishment of bone and periodontal tissue are often reported in the literature and clinical trials using this approach have shown promising results. Stem cells from autologous dental pulp have been successfully applied in bone tissue regeneration using natural collagen scaffold in humans. In addition, revascularization of the root canal already appears in the literature as a promising alternative to apexification. The principle behind this therapy is the use of the blood clot as a scaffold and the migration of stem cells of the apical papilla to regenerate the dental pulp organ. Final considerations: Although still in the early stages, regenerative therapies can now be used in dental practice. Knowledge of the principles governing these therapies should be understood by the dentist for use in clinical practice.
APA, Harvard, Vancouver, ISO, and other styles
39

Zhang, Xiujie, Jingwei Wang, Mingguang Ren, Lifeng Li, Qingwen Wang, and Xiaohua Hou. "A novel collagen/platelet-rich plasma (COL/PRP) scaffold: preparation and growth factor release analysis." Cell and Tissue Banking 17, no. 2 (March 7, 2016): 327–34. http://dx.doi.org/10.1007/s10561-016-9551-z.

Full text
APA, Harvard, Vancouver, ISO, and other styles
40

Özdemir, Erdi, Abdülsamet Emet, Ramin Hashemihesar, Ali Celalettin Sinan Yürüker, Emine Kılıç, Duygu Uçkan Çetinkaya, and Egemen Turhan. "Articular Cartilage Regeneration Utilizing Decellularized Human Placental Scaffold, Mesenchymal Stem Cells and Platelet Rich Plasma." Tissue Engineering and Regenerative Medicine 17, no. 6 (October 8, 2020): 901–8. http://dx.doi.org/10.1007/s13770-020-00298-w.

Full text
APA, Harvard, Vancouver, ISO, and other styles
41

Bolandi, Behzad, Rana Imani, Shahin Bonakdar, and Hossein Fakhrzadeh. "Chondrogenic stimulation in mesenchymal stem cells using scaffold‐based sustained release of platelet‐rich plasma." Journal of Applied Polymer Science 138, no. 12 (October 23, 2020): 50075. http://dx.doi.org/10.1002/app.50075.

Full text
APA, Harvard, Vancouver, ISO, and other styles
42

Barlian, Anggraini, Hermawan Judawisastra, Nayla M. Alfarafisa, Untung A. Wibowo, and Imam Rosadi. "Chondrogenic differentiation of adipose-derived mesenchymal stem cells induced by L-ascorbic acid and platelet rich plasma on silk fibroin scaffold." PeerJ 6 (November 19, 2018): e5809. http://dx.doi.org/10.7717/peerj.5809.

Full text
Abstract:
Articular cartilage is an avascular tissue with limited regenerative property. Therefore, a defect or trauma in articular cartilage due to disease or accident can lead to progressive tissue deterioration. Cartilage tissue engineering, by replacing defective cartilage tissue, is a method for repairing such a problem. In this research, three main aspects—cell, biomaterial scaffold, and bioactive factors—that support tissue engineering study were optimized. Adipose-derived mesenchymal stem cells (ADSC) that become cartilage were grown in an optimized growth medium supplemented with either platelet rich plasma (PRP) or L-ascorbic acid (LAA). As the characterization result, the ADSC used in this experiment could be classified as Mesenchymal Stem Cell (MSC) based on multipotency analysis and cell surface marker analysis. The biomaterial scaffold was fabricated from the Bombyx morii cocoon using silk fibroin by salt leaching method and was engineered to form different sizes of pores to provide optimized support for cell adhesion and growth. Biocompatibility and cytotoxicity evaluation was done using MTT assay to optimize silk fibroin concentration and pore size. Characterized ADSC were grown on the optimized scaffold. LAA and PRP were chosen as bioactive factors to induce ADSC differentiation to become chondrocytes. The concentration optimization of LAA and PRP was analyzed by cell proliferation using MTT assay and chondrogenic differentiation by measuring glycosaminoglycan (GAG) using Alcian Blue at 605 nm wavelength. The optimum silk fibroin concentration, pore size, LAA concentration, and PRP concentration were used to grow and differentiate characterized ADSC for 7, 14, and 21 days. The cell morphology on the scaffold was analyzed using a scanning electron microscope (SEM). The result showed that the ADSC could adhere on plastic, express specific cell surface markers (CD73, CD90, and CD105), and could be differentiated into three types of mature cells. The silk fibroin scaffold made from 12% w/v concentration formed a 500 µm pore diameter (SEM analysis), and was shown by MTT assay to be biocompatible and to facilitate cell growth. The optimum concentrations of the bioactive factors LAA and PRP were 50 µg/mL and 10%, respectively. GAG analysis with Alcian Blue staining suggested that PRP induction medium and LAA induction medium on 12% w/v scaffold could effectively promote not only cell adhesion and cell proliferation but also chondrogenic differentiation of ADSC within 21 days of culture. Therefore, this study provides a new approach to articular tissue engineering with a combination of ADSC as cell source, LAA and PRP as bioactive factors, and silk fibroin as a biocompatible and biodegradable scaffold.
APA, Harvard, Vancouver, ISO, and other styles
43

Alagl, Adel, Sumit Bedi, Khalid Hassan, and Jehan AlHumaid. "Use of platelet-rich plasma for regeneration in non-vital immature permanent teeth: Clinical and cone-beam computed tomography evaluation." Journal of International Medical Research 45, no. 2 (February 23, 2017): 583–93. http://dx.doi.org/10.1177/0300060517692935.

Full text
Abstract:
Objective This study was performed to assess the clinical and radiological outcomes of a revascularization procedure in immature teeth with apical periodontitis using platelet-rich plasma (PRP). The PRP protocol and conventional revascularization protocol, which used a blood clot as the scaffold, were compared. Methods Thirty non-vital immature permanent teeth were randomly categorized into two groups. After disinfecting the root canal space with triple antibiotic paste (1:1:1 ciprofloxacin, metronidazole, and cefaclor), a tissue scaffold was created using either PRP or a blood clot (control) and covered with white mineral trioxide aggregate. All cases were followed up clinically and radiographically for 12 months. Differences in bone density, root length, and lesion size were calculated using preoperative and postoperative computed tomography images. The means of the differences in individual parameters in the blood clot and PRP groups were compared using the Mann–Whitney U test. Results After 5 months, sensitivity tests (cold and electric pulp tests) elicited a delayed positive response in 23 sites. At 12 months, cone-beam computed tomography revealed resolution or a decrease in lesion size and an increase in bone density in all 30 (100%) teeth. Additionally, continued root development was observed in 22 (73%) teeth and early root growth was observed in the test group (mineral trioxide aggregate with PRP). Conclusions The results of this study suggest that PRP can serve as a successful scaffold for regenerative endodontic treatment. With the exception of a significant increase in root length, the results of treatment with PRP were not significantly different from those of the conventional protocol using a blood clot as the scaffold.
APA, Harvard, Vancouver, ISO, and other styles
44

Ahmadipour, Saeedeh, Jaleh Varshosaz, Batool Hashemibeni, Leila Safaeian, and Maziar Manshaei. "Polyhedral Oligomeric Silsesquioxane /Platelets Rich Plasma/Gelrite-Based Hydrogel Scaffold for Bone Tissue Engineering." Current Pharmaceutical Design 26, no. 26 (August 11, 2020): 3147–60. http://dx.doi.org/10.2174/1381612826666200311124732.

Full text
Abstract:
Background: Polyhedral oligomeric silsesquioxane (POSS) is a monomer with silicon structure and an internal nanometric cage. Objective: The purpose of this study was to provide an injectable hydrogel that could be easily located in open or closed bone fractures and injuries, and also to reduce the possible risks of infections caused by bone graft either as an allograft or an autograft. Methods: Various formulations of temperature sensitive hydrogels containing hydroxyapatite, Gelrite, POSS and platelets rich plasma (PRP), such as the co-gelling agent and cell growth enhancer, were prepared. The hydrogels were characterized for their injectability, gelation time, phase transition temperature and viscosity. Other physical properties of the optimized formulation including compressive stress, compressive strain and Young’s modulus as mechanical properties, as well as storage and loss modulus, swelling ratio, biodegradation behavior and cell toxicity as rheometrical parameters were studied on human osteoblast MG-63 cells. Alizarin red tests were conducted to study the qualitative and quantitative osteogenic capability of the designed scaffold, and the cell adhesion to the scaffold was visualized by scanning electron microscopy. Results: The results demonstrated that the hydrogel scaffold mechanical force and injectability were 3.34±0.44 Mpa and 12.57 N, respectively. Moreover, the scaffold showed higher calcium granules production in alizarin red staining compared to the control group. The proliferation of the cells in G4.5H1P0.03PRP10 formulation was significantly higher than in other formulations (p<0.05). Conclusion: The optimized Gelrite/Hydroxyapatite/POSS/PRP hydrogel scaffold has useful impacts on osteoblasts activity, and may be beneficial for local drug delivery in complications including a break or bone loss.
APA, Harvard, Vancouver, ISO, and other styles
45

Masytė, Vestina, Ieva Vaškelytė, and Jurgita Vazgytė. "REGENERACINIŲ ENDODONTINIŲ PROCEDŪRŲ VEIKSMINGUMO PALYGINIMAS, GYDANT NESUSIFORMAVUSIŲ NUOLATINIŲ DANTŲ ŠAKNŲ PULPOS NEKROZĘ." Health Sciences 31, no. 1 (February 6, 2021): 123–28. http://dx.doi.org/10.35988/sm-hs.2021.024.

Full text
Abstract:
Šiuolaikinės regeneracinės endodontinės procedūros skatina naujo vaskuliarizuoto audinio susidarymą kanalo ertmėje, todėl galima tikėtis tolimesnio dantų šaknų formavimosi ir viršūninio periodontito sugijimo. Stebint tokius klinikinius bei rentgenologinius pokyčius, regeneracinės endodontinės procedūros gali būti svarstytinos kaip pirmo pasirinkimo gydymo priemonė, nuolatinių dantų nesusiformavusiose šaknyse išsivysčius pulpos nekrozei. Darbo tikslas – atlikti mokslinės literatūros analizę ir palyginti regeneracines endodontines procedūras taikant PRF, PRP, PP arba kraujo krešulį, kai gydomi nuolatiniai dantys, esant nevisiškai susiformavusioms šaknims ir pulpos nekrozei. Metodika. Atlikta elektroninė literatūros paieška anglų kalba PubMed duomenų bazėje, naudojant šias raktažodžių kombinacijas: tooth regeneration, platelet-rich plasma, platelet-rich fibrin, platelet pellet, scaffold, immature teeth. Rezultatai. Statistiškai reikšmingas danties šaknies pailgėjimas, atlikus kontrolę po vienerių metų su kraujo krešuliu, nustatytas dviejuose tyrimuose, o naudojant PRP – viename. Išvados. Atliekant regeneracines endodontines procedūras, svarbu sukurti natūralų biologinį audinio regeneravimo matriksą, kuris gali būti PRF, PRP, PP ar kraujo krešulio pavidalu. Nėra patvirtintų mokslinių įrodymų, kad danties kanale vietiškai naudojami antibiotikai teigiamai veiktų regeneracines dantų procedūras. EDTA naudojimas nerekomenduojamas.
APA, Harvard, Vancouver, ISO, and other styles
46

Rosadi, Imam, Karina Karina, Komang A. Wahyuningsih, Anggraini Barlian, Iis Rosliana, Tias Widyastuti, Siti Sobariah, and Irsyah Afini. "Biokompatibilitas scaffold sutera asal Bombyx mori ukuran pori 100µm terhadap adipose-derived stem cells (ADSCs) yang dikultur pada berbagai medium pertumbuhan." Jurnal Biologi Udayana 24, no. 1 (June 29, 2020): 7. http://dx.doi.org/10.24843/jbiounud.2020.v24.i01.p02.

Full text
Abstract:
Rekayasa jaringan terdiri dari 3 komponen utama yaitu sel, nutrisi, dan scaffold. Penggunaan sel punca asal jaringan lemak (adipose-derived stem cells/ ADSCs) telah banyak dikembangkan sebagai sumber sel dalam teknologi rekayasa jaringan. Medium yang digunakan dalam mendukung pertumbuhan sel diantaranya medium yang mengandung serum seperti fetal bovine serum (FBS), kombinasi FBS dan L-ascorbic acid 2-phosphate (LAA) atau platelet-rich plasma (PRP). Pada penelitian ini, sutera asal Bombyx mori diproduksi menjadi scaffold sutera ukuran pori 100µm kemudian ADSCs dikultur diatas scaffold dalam medium mengandung 10% FBS, 10% FBS-LAA atau 10% PRP. Uji yang dilakukan adalah uji pertumbuhan ADSCs yang dikultur pada polystyrene kemudian uji biokompabilitas scaffold sutera pada ADSCs dalam medium mengandung 10% FBS, 10% FBS-LAA dan 10% PRP. Hasilnya menunjukkan bahwa ketiga kelompok ADSCs dalam variasi medium yang mengandung FBS, FBS-LAA atau PRP dapat mendukung pertumbuhan sel. Ketiga medium tersebut juga tidak berbeda bermakna antar kelompok pada uji biokompabilitas ADSCs yang dikultur pada scaffold sutera. Berdasarkan hasil tersebut, scaffold sutera berpotensi sebagai substrat ADSCs yang dapat dikembangkan sebagai teknologi rekayasa jaringan.
APA, Harvard, Vancouver, ISO, and other styles
47

Stoker, Aaron, Chantelle Bozynski, Keiichi Kuroki, Kevin Clarke, Jed Johnson, James Cook, and Samuel Franklin. "Comparison of Platelet-Rich Plasma, Stromal Vascular Fraction (SVF), or SVF with an Injectable PLGA Nanofiber Scaffold for the Treatment of Osteochondral Injury in Dogs." Journal of Knee Surgery 31, no. 07 (September 15, 2017): 686–97. http://dx.doi.org/10.1055/s-0037-1606575.

Full text
Abstract:
AbstractStromal vascular fraction (SVF) contains a small number of mesenchymal stem cells and has been used as a treatment for osteoarthritis and cartilage injury. Due to limited evidence of successful cartilage regeneration with injected stem cell therapies, there is interest in combining cellular therapies with injectable scaffolding materials to increase intra-articular residence times of stem cells and improve tissue regeneration. However, the safety of intra-articular injection of SVF combined with injectable scaffolds is unestablished. Also, it is unclear if SVF therapy is superior to more easily prepared biologics, such as platelet-rich plasma (PRP). The purpose of this study was to assess the safety of SVF when combined with an injectable poly(L-lactide-co-glycolide) nanofiber scaffold and to provide a comparison of SVF therapy to PRP. A total of 12 Beagles had osteochondral defects created in both medial femoral condyles and 4 dogs each were allocated to treatment groups of SVF (n = 4), SVF plus PLGA scaffolding (n = 4), or leukoreduced PRP (n = 4). One knee in each dog received treatment, and the contralateral knee was sham treated with saline. Dogs were assessed over a 6-month period, and outcome measures included functional, radiographic, biochemical, and histological assessments. PRP treatment resulted in improvements in lameness scores and objective kinetic assessments of function. There were no statistically significant improvements in function, cartilage biochemical composition, or histology for SVF-treated knees. The combination of SVF and the injectable PLGA scaffold had worse outcomes than other groups including sham treatment based upon functional, biochemical, and histological assessments, raising concerns over the safety of this scaffold for intra-articular injection.
APA, Harvard, Vancouver, ISO, and other styles
48

Anitua, Eduardo, Mar Zalduendo, María Troya, Roberto Tierno, and Mohammad H. Alkhraisat. "The inclusion of leukocytes into platelet rich plasma reduces scaffold stability and hinders extracellular matrix remodelling." Annals of Anatomy - Anatomischer Anzeiger 240 (February 2022): 151853. http://dx.doi.org/10.1016/j.aanat.2021.151853.

Full text
APA, Harvard, Vancouver, ISO, and other styles
49

JANG, CHUL HO, CHEOL HEE CHOI, and YONG BEOM CHO. "Effect of BMP2–Platelet-rich Plasma–Biphasic Calcium Phosphate Scaffold on Accelerated Osteogenesis in Mastoid Obliteration." In Vivo 30, no. 6 (November 4, 2016): 835–40. http://dx.doi.org/10.21873/invivo.11002.

Full text
APA, Harvard, Vancouver, ISO, and other styles
50

Sánchez, Mikel, Eduardo Anitua, Diego Delgado, Peio Sanchez, Roberto Prado, Gorka Orive, and Sabino Padilla. "Platelet-rich plasma, a source of autologous growth factors and biomimetic scaffold for peripheral nerve regeneration." Expert Opinion on Biological Therapy 17, no. 2 (November 28, 2016): 197–212. http://dx.doi.org/10.1080/14712598.2017.1259409.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the bibliographies on the topic 'Platelet rich plasma scaffold' for other source types:
Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography