Relevant bibliographies by topics / Platelet rich plasma scaffold

Academic literature on the topic 'Platelet rich plasma scaffold'

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Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Platelet rich plasma scaffold"

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Mahanani, Erlina Sih, and Fannisa Afrilyana Ulzanah. "Efficacy of Incorporation Platelet Rich Plasma into Gelatine Hydrogel Scaffold between Impregnated and Drop Method." BIO Web of Conferences 41 (2021): 05002. http://dx.doi.org/10.1051/bioconf/20214105002.

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Tissue Engineering which involve three main component such as scaffold, platelet-rich plasma (PRP) and cells is expected to support in bone regeneration. Gelatin hidrogel scaffold is planted have a function as cell environment and PRP provide growth factor to support differentiation of cells. The success of tissue engineering is affected by number of PRP which is contained in scaffold. The purpose of this study is to compare the incorporation process between impregnated and drop method to gelatin hidrogel scaffold. PRP was prepared from three donors of whole blood, and twice sentrifugation by 450 rcf for 5 minutes and 1500 rcf for 7 minutes. PRP was incorporated into 3 gelatin hidrogel scaffolds for each methods. The remnant of PRP which didn’t incorporate were calculated the number of platelet with giemsa stainning. Platelet which loaded were the reduction result of number platelet before incorporate with platelet remnant. Data of the result were analyzed using independent sample t test. Result show the significant was 0.262 (p>0.05) there’s no significane different between impregnated and drop method for incorporating PRP into gelatin hidrogel scaffold. The number of platelet which incorporated in gelatin hidrogel scaffold were effected by characteristic of scaffold such as structure, interface adherence, porosity and swelling ability. The good characteristic of scaffold could be obtain from synthesis and good fabrication technique.
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Shimojo, Andréa Arruda Martins, Amanda Gomes Marcelino Perez, Sofia Elisa Moraga Galdames, Isabela Cambraia de Souza Brissac, and Maria Helena Andrade Santana. "Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine." Scientific World Journal 2015 (2015): 1–12. http://dx.doi.org/10.1155/2015/396131.

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This study aimed to evaluate thein vitroperformance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. The sponges were prepared by controlled freezing (−20, −80, or −196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v). The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum. The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma. The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells. The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a −20°C freezing temperature, while −196°C favored osteogenic differentiation. Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.
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Sancho-Tello, Maria, Sara Martorell, Manuel Mata Roig, Lara Milián, MA Gámiz-González, Jose Luis Gómez Ribelles, and Carmen Carda. "Human platelet-rich plasma improves the nesting and differentiation of human chondrocytes cultured in stabilized porous chitosan scaffolds." Journal of Tissue Engineering 8 (January 1, 2017): 204173141769754. http://dx.doi.org/10.1177/2041731417697545.

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The clinical management of large-size cartilage lesions is difficult due to the limited regenerative ability of the cartilage. Different biomaterials have been used to develop tissue engineering substitutes for cartilage repair, including chitosan alone or in combination with growth factors to improve its chondrogenic properties. The main objective of this investigation was to evaluate the benefits of combining activated platelet-rich plasma with a stabilized porous chitosan scaffold for cartilage regeneration. To achieve this purpose, stabilized porous chitosan scaffolds were prepared using freeze gelation and combined with activated platelet-rich plasma. Human primary articular chondrocytes were isolated and cultured in stabilized porous chitosan scaffolds with and without combination to activated platelet-rich plasma. Scanning electron microscopy was used for the morphological characterization of the resulting scaffolds. Cell counts were performed in hematoxylin and eosin–stained sections, and type I and II collagen expression was evaluated using immunohistochemistry. Significant increase in cell number in activated platelet-rich plasma/stabilized porous chitosan was found compared with stabilized porous chitosan scaffolds. Chondrocytes grown on stabilized porous chitosan expressed high levels of type I collagen but type II was not detectable, whereas cells grown on activated platelet rich plasma/stabilized porous chitosan scaffolds expressed high levels of type II collagen and type I was almost undetectable. In summary, activated platelet-rich plasma increases nesting and induces the differentiation of chondrocytes cultured on stabilized porous chitosan scaffolds.
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Li, Muxin, Rubina Rahaman Khadim, Mitsuru Nagayama, Marie Shinohara, Kousuke Inamura, Mathieu Danoy, Masaki Nishikawa, Katsuko Furukawa, Yasuyuki Sakai, and Toshiki Niino. "Fabrication of a Porous Three-Dimensional Scaffold with Interconnected Flow Channels: Co-Cultured Liver Cells and In Vitro Hemocompatibility Assessment." Applied Sciences 11, no. 6 (March 10, 2021): 2473. http://dx.doi.org/10.3390/app11062473.

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The development of large-scale human liver scaffolds equipped with interconnected flow channels in three-dimensional space offers a promising strategy for the advancement of liver tissue engineering. Tissue-engineered scaffold must be blood-compatible to address the demand for clinical transplantable liver tissue. Here, we demonstrate the construction of 3-D macro scaffold with interconnected flow channels using the selective laser sintering (SLS) fabrication method. The accuracy of the printed flow channels was ensured by the incorporation of polyglycolic acid (PGA) microparticles as porogens over the conventional method of NaCl salt leaching. The fabricated scaffold was populated with Hep G2, followed by endothelization with endothelial cells (ECs) grown under perfusion of culture medium for up to 10 days. The EC covered scaffold was perfused with platelet-rich plasma for the assessment of hemocompatibility to examine its antiplatelet adhesion properties. Both Hep G2-covered scaffolds exhibited a markedly different albumin production, glucose metabolism and lactate production when compared to EC-Hep G2-covered scaffold. Most importantly, EC-Hep G2-covered scaffold retained the antiplatelet adhesion property associated with the perfusion of platelet-rich plasma through the construct. These results show the potential of fabricating a 3-D scaffold with interconnected flow channels, enabling the perfusion of whole blood and circumventing the limitation of blood compatibility for engineering transplantable liver tissue.
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Mahanani, Erlina Sih, Anisya Nur Nova Istiyani, and Ratih Sekar Arum. "Effect of Incorporation Platelet Rich Plasma into Synthetic Coral Scaffold toward Epithelial Thickness of Wound Healing." Key Engineering Materials 884 (May 2021): 267–73. http://dx.doi.org/10.4028/www.scientific.net/kem.884.267.

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Tissue re-epithelialization is a process in wound healing. The healing process of wounds needs to pass through a phase of inflammatory, proliferation, and remodeling. Tissue engineering is the technology service in the field of medicine that can be applied in wound healing. A scaffold is a place for the molecule signal and cell growth to form new tissue. This study aims to investigate the ability of synthetic coral scaffold incorporated PRP in wound healing. Rattus norvegicus were used and divided into 4 groups, synthetic coral scaffold incorporated PRP, scaffold only, Spongostan, and control. The wound healing was observed on 3, 7, and 14 days by measuring the epithelial thickness. The average epithelium thickness on day 3 has not formed. The highest epithelial thickness on days 7 and 14 are obtained from the synthetic coral scaffolds incorporated by PRP. The result of this study presented that Synthetic coral scaffold incorporated by PRP has the highest ability for wound healing and increases the process of tissue regeneration.
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Amoo-Achampong, Kelms, Michael K. Krill, Derrick Acheampong, Benedict U. Nwachukwu, and Frank McCormick. "Evaluating strategies and outcomes following rotator cuff tears." Shoulder & Elbow 11, no. 1_suppl (April 26, 2018): 4–18. http://dx.doi.org/10.1177/1758573218768099.

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Introduction Rotator cuff tear surgical repair techniques have significantly progressed. However, tendon retear following primary repair persistently occurs at high rates. Rehabilitation protocols, surgical fixation techniques, biologic therapy with scaffolds, platelet-rich plasma, and even stem cell applications are under study to promote adequate tendon healing. Methods A nonsystematic query of the PubMed database was conducted in July 2016 utilizing the search terms “rotator cuff repair,” “tear,” “rehabilitation,” “scaffold,” “platelet-rich plasma,” and “stem cell” to identify, analyze, and summarize relevant studies. Conclusion Individualized rehabilitation protocols may be the best approach for small to medium sized tears. Surgical fixation will continue to be debated as modifications to single-row technique and increases in suture number have improved tensile strength. Double-row repairs have been associated with higher costs. Transosseous equivalent technique exhibits comparable subjective and objective outcomes to single- and double-row repair at two-year follow-up. Biocompatible scaffold augmentation has showed inconsistent short-term results. Platelet-rich plasma has lacked uniformity in treatment preparation, administration, and outcome measurement with mixed results. Few human studies have suggested decreased retear rates and improved repair maintenance following bone marrow-derived mesenchymal stem cell augmentation. This review reiterated the necessity of additional high-quality, large-sample studies to develop any final verdict regarding efficacy.
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Rosadi, Imam, Karina Karina, Komang A. Wahyuningsih, Iis Rosliana, Tias Widyastuti, Siti Sobariah, Irsyah Afini, and Anggraini Barlian. "KONDROGENESIS ADIPOSE-DERIVED STEM CELLS MENGGUNAKAN PLATELET-RICH PLASMA PADA SCAFFOLD SUTRA." Al-Kauniyah: Jurnal Biologi 13, no. 1 (April 30, 2020): 31–38. http://dx.doi.org/10.15408/kauniyah.v13i1.12053.

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AbstrakStudi tentang kemampuan adipose-derived stem cells (ADSCs) sebagai sel punca yang dapat berdiferensiasi menjadi kondrosit menggunakan platelet-rich plasma (PRP) sebagai subtitusi fetal bovine serum (FBS) telah banyak dilaporkan. Penggunaan medium pertumbuhan dengan kombinasi ADSCs, PRP dan scaffold sutra masih belum banyak dipelajari dalam rekayasa jaringan kartilago. Studi ini bertujuan untuk mempelajari pengaruh medium yang mengandung 5%, 10% dan 20% PRP terhadap proses kondrogenesis ADSCs manusia yang dikultur pada scaffold sutra Bombyx mori Indonesia. Metode penelitian diawali dengan pembuatan scaffold sutra menggunakan metode salt-leaching, isolasi dan kultur ADSCs manusia dari jaringan lemak, uji pertumbuhan ADSCs pada scaffold sutra dengan variasi konsentrasi PRP pada medium serta analisis kadar glikosaminoglikan (GAG). Hasil penelitian menunjukkan bahwa ADSCs yang dikultur menggunakan PRP lebih tinggi laju pertumbuhannya dibandingkan dikultur menggunakan FBS selama 7 hari pengamatan. Kadar GAG yang disekresikan ADSCs kelompok PRP juga lebih tinggi dibandingkan kelompok FBS. Kadar GAG tertinggi pada hari ke-21 pengamatan adalah medium yang mengandung 20% PRP kemudian 10% dan 5%, sedangkan kadar GAG kelompok kontrol cenderung stabil pada kadar yang rendah. Berdasarkan hasil tersebut, medium yang mengandung PRP memiliki potensi dalam menginduksi kondrogenesis ADSCs yang dikultur pada scaffold sutra.Abstract The studies on adipose-derived stem cells (ADSCs) differentiation into chondrocytes using platelet-rich plasma (PRP) as a substitute for fetal bovine serum (FBS) have been reported. However, the combination of ADSCs, PRP and silk fibroin scaffold has not been widely studied for developing cartilage engineering. Therefore, this research aims to study the effect of medium containing 5%, 10% and 20% PRP towards chondrogenesis of human ADSCs cultured on silk fibroin scaffold from Indonesia Bombyx mori. At first, the silk fibroin scaffold was fabricated using a salt-leaching method, then ADSCs were isolated and cultured from adipose tissues. The assays of growth curve and biocompatibility of silk fibroin scaffold toward ADSCs supplemented by PRP as well as glycosaminoglycans (GAG) concentration were conducted later. The results showed that higher absorbance of proliferation rate was on ADSCs supplemented by various PRP concentrations compare to FBS control group for seven days of observation. Level of GAG, which secreted by ADSCs supplemented by a various concentration of PRP, was also higher than the FBS group. The highest level of GAG on day 21 was observed in 20% PRP group then 10% and 5% PRP, while a group of GAG level is stable at low levels. This study concludes that PRP has the potential to induce chondrogenesis ADSCs which cultured on silk fibroin scaffold.
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Utomo, Dwikora Novembri, and Anthoni Yusbida. "CYTOTOXIC EFFECT OF FREEZE DRIED BOVINE CARTILAGE POWDER AND PLATELET RICH PLASMA (PRP) TO MESENCHYMAL STEM CELL (MSCs)." (JOINTS) Journal Orthopaedi and Traumatology Surabaya 6, no. 2 (December 9, 2019): 63. http://dx.doi.org/10.20473/joints.v6i2.2017.63-70.

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Cartilage repair is a challenging clinical problem because the damage is an irreversible condition. Many studies had been performed using several kinds of natural or synthetic scaffold. Attempts to repair articular cartilage using scaffold usually found many problems, lacks the physical structure and mechanical properties necessary to ensure long-term efficacy to cartilage defect. Furthermore, scaffold frequently cause toxicity to the host. Therefore, this study was performed in vitro to test the toxicity effect of scaffold freeze dried bovine cartilage powder and platelets Rich Plasma (PRP). This research was conducted using pure experimental research design in 4 groups of animal stem cells which being added with scaffold freeze dried bovine cartilage scaffold provided with platelet rich plasma. This study using posttest only control group design. The result being processed with MTT assay and spectrophotometer for counting the viable stem cells. There was no significant difference in the amount of macrophage between control and the freeze dried bovine cartilage scaffold provided with PRP (p=0,128). With this result in the number of macrophages between the control with freeze dried bovine cartilage scaffold provided PRP, it can be concluded that these biomaterials have biocompatibility.
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Latifi, Mona, Tahereh Talaei-Khozani, Hossein Mehraban-Jahromi, Mahsa Sani, Mahmood Sadeghi-Atabadi, Abbas Fazel-Anvari, and Maryam Kabir-Salmani. "Fabrication of platelet-rich plasma heparin sulfate/hydroxyapatite/zirconia scaffold." Bioinspired, Biomimetic and Nanobiomaterials 7, no. 2 (June 2018): 122–30. http://dx.doi.org/10.1680/jbibn.17.00038.

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Houdek, Matthew T., Cody C. Wyles, Paul G. Stalboerger, Andre Terzic, Atta Behfar, and Steven L. Moran. "Collagen and Fractionated Platelet-Rich Plasma Scaffold for Dermal Regeneration." Plastic and Reconstructive Surgery 137, no. 5 (May 2016): 1498–506. http://dx.doi.org/10.1097/prs.0000000000002094.

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Dissertations / Theses on the topic "Platelet rich plasma scaffold"

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Talamas, Ugalde Lucet Vanessa. "Effects of platelet rich plasma on marrow stromal cells differentiation seeded on three dimensional scaffolds." To access this resource online via ProQuest Dissertations and Theses @ UTEP, 2008. http://0-proquest.umi.com.lib.utep.edu/login?COPT=REJTPTU0YmImSU5UPTAmVkVSPTI=&clientId=2515.

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Fontenot, Robin L. "Tube centrifugation for processing platelet-rich plasma in the horse." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/76971.

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Platelet-rich plasma (PRP) is a popular treatment for equine tendon and ligament injuries; however, commercial PRP systems are expensive. Development of a safe, inexpensive alternative would make PRP therapy more widely available to horse owners. The purpose of this study was to evaluate the quality and bacteriologic safety of PRP produced by three simple, inexpensive tube centrifugation methods and compare the results to a commercial system. Citrated blood collected from 26 normal horses was processed by four methods: blood collection tubes centrifuged at 1200 and 2000 x g, a 50ml conical tube, and a commercial system. Platelet and cell counts and mean platelet volume (MPV) in whole blood and PRP were determined using an automated hematology analyzer. Results were analyzed using mixed model ANOVA with post-hoc comparisons (MPV and fold change for RBC, WBC, and platelets) and binary logistic generalized estimating equations with horse as a blocking factor (absolute numbers of WBC, and platelets). Aerobic and anaerobic cultures were performed. Significance was set at p<0.05. Mean platelet concentrations ranged from 1.55 to 2.58 fold. The conical tube method produced the highest number of PRP samples with platelet concentrations of greater than 2.5-fold and within the clinically acceptable range of >250,000 platelets/?l. WBC counts were lowest using the commercial system and unacceptably high using the red top methods. The incidence of bacterial contamination was low (2.1%). Based on these results, the conical tube method may be a suitable alternative to commercial PRP systems in cases with budgetary constraints.
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Thor, Andreas. "On platelet-rich plasma in reconstructive dental implant surgery /." Göteborg : Departments of Biomaterials and Oral & Maxillofacial Surgery, the Sahlgrenska Academy at Göteborg University and the Department of Surgical Sciences, Oral & Maxillofacial Surgery, Uppsala University Hospital, 2006. http://hdl.handle.net/2077/745.

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O'Shea, Caitlin Mary. "Comparison of platelet counting technologies in equine platelet concentrates." Thesis, Virginia Tech, 2014. http://hdl.handle.net/10919/56837.

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Platelet rich plasma (PRP) is a popular autologous biological therapy used for the treatment of various equine ailments, including tendon and ligament injuries, osteoarthritis, and cutaneous wounds. A number of commercial products are available for producing PRP, each generating a slightly different product. Variations in platelet numbers and white blood cell (WBC) counts are believed to be the most critical variables, as they are directly related to concentrations of growth factors and inflammatory cytokines. Accurate documentation of platelet numbers is essential for prospective evaluation of clinical outcomes, but can be problematic in platelet concentrates depending on the counting method employed. The objectives of this study were to compare the performance of four platelet counting technologies in equine platelet concentrates and to evaluate the ability of the Magellan PRP system to concentrate equine platelets. We hypothesized that there would be no differences in platelet counts among the four counting technologies and that the Magellan system would generate platelet concentrations greater than 500,000/μL. Citrated whole blood was collected from 32 horses and platelet, WBC, and red blood cell concentrations were measured using a commercial hematology analyzer (Advia 2120) prior to preparation of PRP using the Magellan system. Platelets were quantified in individual identical aliquots of equine PRP produced by the Magellan system (n=32) using three different technologies: optical scatter (Advia 2120), impedance (CellDyn 3700), and hand count using direct microscopy (Thrombo-TIC). An immunofluorescent counting method was performed on a subset of 15 of the 32 samples using a mouse monoclonal anti-sheep antibody against integrin alpha αIIbβ₃ (anti-CD41/CD61) and a fluorescent secondary antibody. Measured platelet concentrations were compared using Passing and Bablok regression analyses and mixed model ANOVA. The Magellan PRP system yielded mean (± SD) platelet and WBC counts of 893,090 ± 226,610/μL and 35,806 ± 9,971/μL, respectively. Platelet counts generated by optical scatter were consistently higher than those generated by impedance. Systematic and proportional biases were observed between these two automated methods. No bias (systematic or proportional) was observed among any of the other counting methods. Despite the bias detected between the two automated systems, there were no significant differences on average among the four counting methods evaluated, based on the ANOVA. All four platelet counting methods tested are therefore suitable for quantifying platelets in equine PRP for clinical applications. The Magellan PRP system consistently generated desirably high platelet concentrations as well as higher than expected WBC concentrations. The high platelet concentrations served as a good test medium for the study; however, the concurrent high WBC counts may be undesirable for selected orthopedic applications.
Master of Science
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Baboldashti, Nasim Zargar. "Platelet rich plasma and mechanical loading in regenerative tendon repair." Thesis, University of Oxford, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.556118.

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Abstract Tendon injuries and tendinopathy are a growing problem in the aging but physically active population as well as athletes. Tendons have highly ordered matrix and undergo complex changes during the remodelling phase of tendon healing. Moreover, anaerobic metabolism and poor vascular network contribute to slow adaptation of tissue to the remodelled matrix which consequently results in slow and compromised healing. Such a destitute and slow healing process necessitates development of new and effective therapies and to combine therapies to obtain possibly synergistic effects. Addressing this clinical requirement, the work presented in this thesis investigates the role of two emerging treatment options, platelet rich plasma (pRP) and mechanical loading, on tendon healing. The effects of PRP, a rich autologous source of growth factors, on tendon cells was studied by modelling important stages of tendon healing in vitro. Key parameters such as cellular migration, chemotaxis, viability and senescence were investigated by means of different culturing and staining techniques together with microscopic analyses. PRP significantly increased migration and chemotaxis in human pnmary tenocyte culture. Moreover, PRP protected human tenocytes against challenging environments created by known tendon damaging drugs, dexamethasone and, ciprofloxacin, as well as the injury relevant condition of hypoxia. 11 Concurrently, an in vitro rat tail tendon injury model and static loading device was developed to assess the effect of static mechanical loading and PRP on the biochemical and biomechanical properties of tendon at the tissue level. This in vitro system was also used to investigate the synergistic effects of PRP and mechanical loading on tendon healing. Both PRP and mechanical loading helped to improve the biomechanical and biochemical properties of damaged tendon in vitro. In conclusion, the positive effects of PRP on key cellular parameters such as cell survival, migration and chemotaxis and also mechanical and biochemical properties of tendon tissue make it an important option for faster and less invasive tendon treatment. Additionally, an in vitro tendon injury model together with the mechanical loading device provide a new tool to investigate the mechanical boundary conditions suitable for treating different types of tendon disorders. The findings from the current study points towards the. significant contribution of PRP and mechanical loading to the healing process in tendons and could serve as a promising starting point for developing integrated therapeutic modalities to improve the quality and speed of recovery from tendon injury. 111.
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Cundell, Jill Hannah. "Evaluation of the characteristics of leucocyte-rich platelet-rich plasma from participants with diabetes." Thesis, Ulster University, 2016. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.692821.

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The aim of this research was to investigate the characteristics of leucocyte- rich platelet-rich plasma (LR-PRP) from three groups of participants; healthy diabetics, healthy controls and a group of diabetics with an active foot ulcer. ELISAs were used to measure the concentrations of a number of intrinsic growth factors in LR-PRP. No significant differences were found between groups for the levels of any growth factor. The pH and redox potential of the unsupplemented LR-PRP from the three groups of study participants were determined using a hand-held ArrowDOX pH/redox meter. There was no significant difference in the pH values or redox potential found between the three groups of participants. Experiments using a well diffusion assay and seeded lawns of bacteria frequently identified in diabetic foot infections were conducted to investigate antimicrobial properties of either pure or supplemented LRPRP. The inherent antimicrobial properties of LR-PRP were measured. Some plates which had lawns of Staphylococcus aureus, MRSA, Streptococcus pyogenes, Proteus vulgaris and E.coli had zones of inhibition caused by LR- PRP from all three study groups. Antibiotics (co amoxiclav, clindamycin, doxycycline and pip I taz) frequently used to manage diabetic foot infections were used to supplement the LR-PRP. Zones of inhibition were found for the dilutions in LR-PRP for some of the antibiotics against some bacteria, which were greater than the zones of inhibition obtained for LR - PRP and Ringers solution alone. Some differences were found in size of the ZOI between participant groups. The LR -PRP encouraged enhanced bacterial growth on the plates with Pseudomonas aeruginosa indicating that clinicians should consider assessing the wound for the presence of this organism prior to application. Enhanced bacterial growth rather than inhibited bacterial growth was found in all three study groups, with all challenge bacteria used when LR-PRP was supplemented with insulin.
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Herrmann, Sebastian [Verfasser]. "Quantifizierung von Wachstumsfaktoren in Bohrmehl, Beckenkamm-Spongiosa, Platelet Poor und Platelet Rich Plasma / Sebastian Herrmann." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2015. http://d-nb.info/1076270794/34.

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Wang, Xiao. "Tendon tissue engineering using skills and platelet rich plasma : A multidisciplinary strategy." Thesis, University of Oxford, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.533891.

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Yavorskyi, A. V. "Clinical efficiency of platelet rich plasma use in patients with odontogenous jaw." Thesis, БДМУ, 2020. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/17837.

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Alsousou, Joseph. "Platelet rich plasma in regenerative tendon repair mechanobiological interactions and clinical application." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:2692c00e-0c0d-4096-8daa-c00ad47fd03d.

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Tendon injury prevalence in both athletic and occupational settings is on the increase. Tendon healing properties are poor, despite the complex biological process triggered by the injury, which makes those injuries incapacitating for months. A significant proportion of these injuries remain difficult to treat, and many patients suffer from decreased performance and longstanding sequelae. While mechanical stabilization has been a hallmark of tendon surgical management, orthobiologics are playing an increasing role in optimizing tendon healing. Platelet rich Plasma (PRP), which is a volume of autologous plasma having platelet concentration above baseline, has been suggested as an accelerant orthobiological agent rich in supraphysiological concentration of growth factors. However, strong evidence of its mode of action and of its clinical efficacy is lacking. The purpose of this thesis is to identify the role of PRP in tendon regeneration in in-vitro and clinical studies. Firstly, the viability and biological components of clinically-prepared PRP were studied in novel experiments. This PRP was used in linked in-vitro studies to investigate the possible mechanism of PRP effect on the injured Achilles tendon cells and tissues. Cell count, viability, proliferation and DNA content were studied. The clinical application of PRP in Achilles tendon rupture was assessed in a randomised clinical pilot study using a combination of PROMs, objective outcome measures and a novel imaging modality called functional ultrasound elastography. This non-invasive technique was developed in a healthy-tendons volunteer study and its feasibility in ruptured tendons was assessed in the pilot trial. In another unique study, the immunohistochemical response to PRP was assessed in biopsies taken under US guidance at week 6 and compared to control to explore the possible mechanism of PRP effects. The findings confirmed that PRP is a viable activatable autologous blood product rich in growth factors. The results also confirmed that leukocytes and platelets are present in very high concentration with reversal of lymphocyte neutrophil ratio. Elastography volunteer study confirmed that FUSE is feasible using clinically applicable ultrasound scan. The improved algorithm allowed visualisation of localised strain within the studied tissues. The clinical application of PRP in Achilles tendon rupture revealed positive efficacy signal that PRP led to faster healing, improved pain and earlier restoration of function. However, the findings of this pilot trial were indicative and not confirmative. Immunohistochemistry analysis showed that PRP enhanced the maturity of the healing tendon tissues by promoting better collagen I deposition, improved Collagen III/Collagen I ratio, reduced cellularity, better vascular structure and higher GAGs content when compared with control. The finding may explain the clinical improvement observed in these patients at week 6 onwards. Linked in-vitro studies showed that autologous PRP with its cellular components, which include platelets, leukocytes and erythrocytes, has the ability to stimulate tendon cell migration to the injury site and stimulate proliferation in the injured human tendon. Additionally, it may maintain tissue viability in the hypoxic environment that follows tendon injury. Promoting migration and proliferation of cells and maintain tissue viability may play an important role to accelerate tendon healing. The findings of this project has informed the design of a phase II large multi-centre randomised controlled trial and helped secure major funding from the National Institute of Health Research (NIHR). This trial will set the scene for PRP use in tendon treatment.
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Books on the topic "Platelet rich plasma scaffold"

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Lana, José Fábio Santos Duarte, Maria Helena Andrade Santana, William Dias Belangero, and Angela Cristina Malheiros Luzo, eds. Platelet-Rich Plasma. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-40117-6.

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Ferneini, Elie M., Regina Landesberg, and Steven Halepas, eds. Platelet Rich Plasma in Medicine. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-94269-4.

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Maffulli, Nicola, ed. Platelet Rich Plasma in Musculoskeletal Practice. London: Springer London, 2016. http://dx.doi.org/10.1007/978-1-4471-7271-0.

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Sadick, Neil S., ed. Platelet-Rich Plasma in Dermatologic Practice. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-66230-1.

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Khetarpal, Shilpi, ed. Aesthetic Clinician's Guide to Platelet Rich Plasma. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-81427-4.

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Lee, Linda N., ed. Hair Transplant Surgery and Platelet Rich Plasma. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-54648-9.

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Anitua, Eduardo, Ramón Cugat, and Mikel Sánchez, eds. Platelet Rich Plasma in Orthopaedics and Sports Medicine. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-63730-3.

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Springer. Platelet-Rich Plasma. Springer London, Limited, 2013.

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Khwarg, Juewon, Daniel A. Fung, Corey Hunter, and Timothy T. Davis. Platelet-Rich Plasma Injections. Edited by Mehul J. Desai. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199350940.003.0028.

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Platelet-rich plasma (PRP) is an autologous plasma suspension enriched with a supraphysiologic concentrate of platelets, isolated through a process of centrifugation. Administered locally (usually by injection or direct application) to areas of injury, PRP contains a high density of growth factors, which are believed to potentiate the body’s natural regenerative processes. Over the past 20 years, interest in PRP therapy has grown exponentially, as it offers a relatively safe, autologous treatment modality. It has gained particular popularity for a wide variety of musculoskeletal pathologies. There is a growing body of scientific literature that is giving further insight into PRP’s therapeutic effects. This chapter will review the history, preparation techniques, basic science justifications, current clinical evidence, as well as procedural considerations for the therapeutic use of PRP.
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Platelet-Rich Plasma in Dermatologic Practice. Springer International Publishing AG, 2021.

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Book chapters on the topic "Platelet rich plasma scaffold"

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Souza, Dmitri. "Platelet-Rich Plasma." In Ultrasound for Interventional Pain Management, 317–24. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-18371-4_25.

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Özgürtaş, Taner, Burkay Utku, and Cemil Yildiz. "Platelet-Rich Plasma." In Musculoskeletal Research and Basic Science, 283–88. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-20777-3_16.

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Yuan, Xiaoning (Jenny), and Alfred C. Gellhorn. "Platelet-Rich Plasma." In Regenerative Medicine for Spine and Joint Pain, 55–86. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-42771-9_5.

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Trebinjac, Suad, and Manoj Kumar Nair. "Platelet Rich Plasma." In Regenerative Injections in Sports Medicine, 51–59. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-6783-4_7.

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Hunter, Corey W., Timothy Davis, and Priyal Fadadu. "Platelet-Rich Plasma." In Advanced Procedures for Pain Management, 443–57. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-68841-1_38.

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Miranda-Grajales, Hector. "Platelet-Rich Plasma." In Treatment of Chronic Pain Conditions, 183–84. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6976-0_50.

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Lana, José F., Adam Weglein, Eduardo Vicente, Amanda G. M. Perez, Ana A. Rodrigues, Ângela C. M. Luzo, Maria H. A. Santana, and William D. Belangero. "Platelet Rich Plasma and Its Growth Factors: The State of the Art." In Platelet-Rich Plasma, 1–59. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-40117-6_1.

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Chao, Linda, Martha M. Murray, and Patrick Vavken. "Platelet-Rich Plasma (PRP) in Ligament and Tendon Repair." In Platelet-Rich Plasma, 215–30. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-40117-6_10.

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Gobbi, Alberto, Georgios Karnatzikos, Somanna Malchira, and Anup Kumar. "Platelet Rich Plasma (PRP) in Osteoarthritis." In Platelet-Rich Plasma, 231–36. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-40117-6_11.

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Tarczyńska, Marta, and Krzysztof Gaweda. "PRP in the Ambulatory Therapy of Tendinopathy of the Elbow, Knee and Foot." In Platelet-Rich Plasma, 237–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-40117-6_12.

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Conference papers on the topic "Platelet rich plasma scaffold"

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Mahanani, Erlina Sih, Nur Farda, Inten Tejaningasih, and Nadya Khairunissa. "The Effects of Platelet Rich Plasma Incorporation Towards Swelling Profile and Gel Fraction of Synthetic Coral Scaffold." In 2018 1st International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BioMIC). IEEE, 2018. http://dx.doi.org/10.1109/biomic.2018.8610535.

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Grabowski, F. E. "RHEOLOGY AND PRIMARY HEMOSTASIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643986.

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Overview The adhesion-aggregation of platelets to a site of vessel wall injury is a quintessential blood flow phenomenon. Firstly, platelets are driven to the vicinity of the vessel wall by a form of convective diffusion in which red cells both mechanically augment the effective platelet diffusivity (Turitto et al., Ind. Eng. Chem. Fund. 11:216-223, 1972; Grabowski et al., Ind. Eng. Chem. Fund. 11:224-232, 1972) and enhance the near-wall piatelet concentration (Ti11es and Eckstein, Microvasc Res., In press, 1987). Secondly, red cells subjected to physiologic shear forces are capable of secreting sufficient adenine nucleotides to induce primary platelet aggregation without themselves undergoing frank lysis (Reimers et al, Blood 64:1200-1206, 1984). This "humoral" effect of erythrocytes is likely to contribute to primary hemostasis in a shear stress-dependent manner. Thirdly, endothelial cells are able to modulate platelet aggregation at a site of vessel injury by producing prostacyclin (and perhaps other antithrombotic substances) in a manner which increases with vessel shear rate (Grabowski et al, Blood 62:301a, 1983); production for a large range of arterial shear rates appears to be limited by plasma-borne substrate (arachidonate). This manner of production ensures a concentration of prostacyclin in the near-wall region which remains relatively independent of shear rate.Imaging primary hemostasis. In our work, epi-fluorescence videomicroscopy has allowed real time imaging of platelet adhesion-aggregation to a simulated vessel wall injury. The injury model is an endothelial cell monolayer (ECM) across which, prior to ECM exposure to flowing blood, a 6-0 sterile suture is drawn in a direction transverse to flow. Microinjuries result which measure 70 ± 15μm (Mean ± SD) in width. The fluorescent label is the TAB murine monoclonal antibody (courtesy of Dr. R.P. McEver) directed against human platelet GPIIB, together with a fluorescein-conjugated goat F(ab')2 against murine inmunoglobulin. The injured ECM's, grown to confluence on rectangular cover glasses precoated with microfibrillar collagen, comprise one wall of a flow chamber mounted on a vertical microscope stage. On microinjury sites and at shear rates of 100 to 700 sec-1, computer-enhanced video images show adherence, remodelling and growth of chains of platelet aggregates. Aligned with the flow direction, these chains have a spacing of approximately 30)im, a length similar to the average endothelial cell diameter. One may speculate that such chains provide a scaffold for wound healing insofar as they are likely rich in agents chemotactic for leukocytes and in platelet-derived growth factor.Modulatory role of endothelium. When the ECM's are pre treated with 1.0 mM FC lysine acetyl sal icy late (LA), aggregate length increases (P<0.001) up totwo-fold, outflow levels by RIA of serum thromboxane B2 increase (8 of 8 paired runs), and outflow levels of prostacyclin by RIA for 6-Keto PGFiot decrease (5 of 7 paired runs). The Table gives data for one of four similar experiments at 270 sec-1 and following five minutes of flow. These data imply that products of ECM which are inhibitable by aspirin modulate local adhesion-aggregation; their inhibition, as by vasculitis or drugs, may give rise to thrombotic states.Bleeding disorders. Aggregate length is reduced in von Willebrand's disease (4 patients), Hermansky-Pudlak syndrome (2 patients), and after 300 mg oral aspirin (Tablet 4 donors). The reduction in the first two, however, is greater (P<0.01) than that for oral aspirin. With oral aspirin, further, there is a paradoxic increase in the percent platelet coverage of the injury area. Summary. Rheology has profound effects on the rate, structure, and modulation of primary hemostasis. Many of these effects can be studied via real-time, epi-fluorescence videomicroscopy of platelet adhesion-aggregation to a site of injury to an endothelial cell monolayer exposed to flowing blood. The model described has application to the study of thrombotic and hemostatic disorders and unstable angina.
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Smagin, M. A., A. Iu Demura, O. A. Shumkov, M. Iu Soluyanov, O. V. Poveshenko, and V. V. Nimaev. "Platelet-rich plasma in treatment of non-healing ulcers." In 2018 11th International Multiconference Bioinformatics of Genome Regulation and Structure\Systems Biology (BGRS\SB). IEEE, 2018. http://dx.doi.org/10.1109/csgb.2018.8544810.

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Bajpai, Sanchit. "Study on Use of Platelet-Rich Plasma in Myringoplasty." In 27th Annual National Conference of the Indian Society of Otology. Thieme Medical and Scientific Publishers Private Ltd., 2019. http://dx.doi.org/10.1055/s-0039-1700235.

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Wienaldi, Wienaldi, I. Nyoman Ehrich Lister, and Bungaran Bungaran. "EFFECTS OF ANTIOXIDANT ACTIVITY IN THE PLATELET RICH PLASMA." In The 8th International Conference on Public Health 2021. Masters Program in Public Health, Universitas Sebelas Maret, 2021. http://dx.doi.org/10.26911/ab.medicine.icph.08.2021.42.

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DeMello, V., G. Chen, J. Wakshlag, and D. Mason. "Consistency of Platelet Counts within Individual Patients during Platelet Rich Plasma Generation and Effects of Freezing on the Concentration of Growth Factors in Platelet Rich Plasma." In Abstracts of the 6th World Veterinary Orthopedic Congress. Georg Thieme Verlag KG, 2022. http://dx.doi.org/10.1055/s-0042-1758295.

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Taufik S, Ahmad, Adnanto Wiweko, Devi Rahmadona, Dyah Purnaning, Mohammad Rizki, and Bayu Tirta Dirja. "Cell Multiplication in Platelet Rich Fibrin (PRF) and Platelet Rich Plasma (PRP) as Biofuels for Cartilage Tissue Engineering." In 2nd Global Health and Innovation in conjunction with 6th ORL Head and Neck Oncology Conference (ORLHN 2021). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/ahsr.k.220206.062.

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Sufarnap, Erliera, Ervina Sofyanti, and Syafrudin Ilyas. "The Effect of Platelet-Rich Plasma to Orthodontic Tooth Movement." In International Dental Conference of Sumatera Utara 2017 (IDCSU 2017). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/idcsu-17.2018.22.

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Villanova Lopez, MDM, M. Nunez-Nunez, M. Murillo-Izquierdo, J. Donaire, R. Alvarez-Sanchez, M. Camean-Fernandez, and S. Sandoval. "4CPS-280 Does platelet-rich plasma composition matter in hip osteoarthritis?" In 24th EAHP Congress, 27th–29th March 2019, Barcelona, Spain. British Medical Journal Publishing Group, 2019. http://dx.doi.org/10.1136/ejhpharm-2019-eahpconf.429.

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Wang, James H.-C. "Tendon Stem Cells and Platelet-Rich Plasma for Repair of Injured Tendons." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53204.

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Tendon injuries, including acute tendon injuries and tendinopathy, are common in both occupational and athletic settings. However, current treatments for tendon injury are largely ineffective, as they cannot restore normal structure and function to injured tendons. This challenge mainly stems from our incomplete understanding of tendon cell properties and responses to biomechanical and biochemical environments surrounding the cells. In recent years, however, significant progress has been made on two fronts. First, tendon stem cells (TSCs) have been recently identified. The tendon-specific stem cells can self-renew and posses multi-differentiation potential and as such, may be used to repair injured tendons more effectively. Second, platelet-rich plasma (PRP) has now been widely used in orthopaedics and sports medicine to treat injured tendons. In this presentation, I will present data on TSCs, in terms of their differential properties with respect to tenocytes and their differential mechano-responses when subjected to small and large mechanical loading conditions. I will also discuss the basic scientific studies on PRP regarding its effects on TSCs, particularly on their differentiation, which is a critical issue related to the safety and efficacy of PRP treatment in clinics (Fig. 1).
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Reports on the topic "Platelet rich plasma scaffold"

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Harna, Bushu, Vinay Gupta, Shivali Arya, Naveen Jeyaraman, Ramya Lakshmi Rajendran, Madhan Jeyaraman, Manish Khanna, Chae Moon Hong, Byeong-Cheol Ahn, and Prakash Gangadaran. Efficacy of Platelet-rich Plasma in Adhesive Capsulitis of Shoulder: A Systematic Review. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, November 2022. http://dx.doi.org/10.37766/inplasy2022.11.0127.

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Yu, Hongwei, Zhaohong Zhou, Bin Yu, Tianwei Sun, Qiong Tang, and Yutao Jia. The efficacy of platelet-rich plasma applicated in spinal fusion surgery: a meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, May 2022. http://dx.doi.org/10.37766/inplasy2022.5.0055.

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wu, wei-yong, and weiguo xu. Arthroscopic microfracture combined with platelet rich plasma in the treatment of cartilage injury：a Meta analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, March 2022. http://dx.doi.org/10.37766/inplasy2022.3.0107.

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Song, Yujie, Xiao'ao Xue, Qianru Li, and Yinghui Hua. Platelet-rich plasma injections in the treatment of tendinopathy: A systematic review with network meta-analyses. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, September 2020. http://dx.doi.org/10.37766/inplasy2020.9.0024.

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Gupta, Aditya, Mesbah Talukder, and Mary Bamimore. Monotherapy with platelet-rich plasma for male and female pattern baldness: protocol for a systematic review. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, July 2021. http://dx.doi.org/10.37766/inplasy2021.7.0088.

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Zhang, Qing, Yi-jia Huang, Yuan Gu, Yi-he Dong, and Jiang-dong Ni. Platelet-Rich Plasma combined core decompression for treatment of osteonecrosis of the femoral head: A meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, November 2021. http://dx.doi.org/10.37766/inplasy2021.11.0098.

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Yu, Shanzi. A systematic review and meta-analysis of the efficacy of platelet-rich plasma injection in the treatment of frozen shoulder. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, June 2020. http://dx.doi.org/10.37766/inplasy2020.6.0097.

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Pepelassi, Eudoxie, Nikolaos Markou, Sotirios Kotsovilis, Harry Charalabos Stamatakis, and Ioannis Vrotsos. The Use of Platelet-Rich Plasma Alone in The Treatment of Periodontal Endosseous Defects: A Report of Two Clinical Cases. Science Repository, September 2019. http://dx.doi.org/10.31487/j.dobcr.2019.04.02.

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Ge, Rongrong, shuying Chen, Jiawang Jiang, Bangmin Wang, You Zeng, Qianan Cao, Yunfeng Liu, and Jun Wu. Platelet Rich Plasma Versus Glucocorticoid for Plantar Fasciitis: A protocol for systematic review and meta-analysis of randomized controlled trials. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, October 2021. http://dx.doi.org/10.37766/inplasy2021.10.0067.

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Wang, Bing Wang, Yongjie Ye, Ziyi Wang, Zhiqiang Wang, Ancheng Wei, Long Yao, and Xin Huang. Efficacy and safety of intra-articular injection of platelet-rich plasma for frozen shoulder: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, October 2022. http://dx.doi.org/10.37766/inplasy2022.10.0095.

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