Journal articles: 'Plasma characterisation'

1

Kotsonis, Marios. "Diagnostics for characterisation of plasma actuators." Measurement Science and Technology 26, no. 9 (August 13, 2015): 092001. http://dx.doi.org/10.1088/0957-0233/26/9/092001.

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2

Krüger, S., R. D. Schulze, K. Brademann-Jock, S. Swaraj, and J. Friedrich. "Characterisation of plasma polymers by thermoluminescence." Surface and Coatings Technology 201, no. 3-4 (October 2006): 543–52. http://dx.doi.org/10.1016/j.surfcoat.2005.12.003.

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3

Outred, M., and E. Surrey. "Characterisation of a microwave-induced argon plasma." Journal of Physics B: Atomic and Molecular Physics 20, no. 19 (October 14, 1987): 5241–53. http://dx.doi.org/10.1088/0022-3700/20/19/032.

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4

Ray, P. K. "Characterisation of plasma in a rail gun." IEE Proceedings A Physical Science, Measurement and Instrumentation, Management and Education, Reviews 133, no. 1 (1986): 38. http://dx.doi.org/10.1049/ip-a-1.1986.0004.

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5

Dahl, S., D. Rats, J. von Stebut, L. Martinu, and J. E. Klemberg-Sapieha. "Micromechanical characterisation of plasma treated polymer surfaces." Thin Solid Films 355-356 (November 1999): 290–94. http://dx.doi.org/10.1016/s0040-6090(99)00514-3.

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6

Scharwitz, Christian, Marc Böke, Suk-Ho Hong, and Jörg Winter. "Experimental Characterisation of the Plasma Absorption Probe." Plasma Processes and Polymers 4, no. 6 (August 23, 2007): 605–11. http://dx.doi.org/10.1002/ppap.200700047.

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7

Bowfield, Andrew, Josephine Bunch, Tara L. Salter, Rory T. Steven, Ian S. Gilmore, Dave A. Barrett, Morgan R. Alexander, Kirsty McKay, and James W. Bradley. "Characterisation of a micro-plasma for ambient mass spectrometry imaging." Analyst 139, no. 21 (2014): 5430–38. http://dx.doi.org/10.1039/c4an01110d.

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8

Sun, Y., N. Luo, and T. Bell. "THREE-DIMENSIONAL CHARACTERISATION OF PlASMA NITRIDED SURFACE TOPOGRAPHY." Surface Engineering 10, no. 4 (January 1994): 279–86. http://dx.doi.org/10.1179/sur.1994.10.4.279.

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9

Robledo-Martinez, A., A. Villarreal-Garcia, L. Miranda, and M. R. Hernández. "Characterisation of a Tungsten X-pinch dense plasma." Journal of Physics: Conference Series 792 (January 2017): 012042. http://dx.doi.org/10.1088/1742-6596/792/1/012042.

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10

Benea, M. L., and L. P. Benea. "Characterisation of the TiO2coatings deposited by plasma spraying." IOP Conference Series: Materials Science and Engineering 106 (February 1, 2016): 012024. http://dx.doi.org/10.1088/1757-899x/106/1/012024.

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11

Gredelj, Sabina, Andrea R. Gerson, Sunil Kumar, and N. Stewart McIntyre. "Plasma nitriding and in situ characterisation of aluminium." Applied Surface Science 199, no. 1-4 (October 2002): 234–47. http://dx.doi.org/10.1016/s0169-4332(02)00841-3.

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12

Anghel, S. D., A. Simon, A. I. Radu, and I. J. Hidi. "Spectroscopic characterisation of a cross-flow plasma jet." Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 267, no. 2 (January 2009): 430–33. http://dx.doi.org/10.1016/j.nimb.2008.10.026.

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13

Voulot, D., R. W. McCullough, W. R. Thompson, D. Burns, J. Geddes, G. J. Cosimini, E. Nelson, P. P. Chow, and J. Klaassen. "Characterisation of an RF atomic nitrogen plasma source." Journal of Crystal Growth 201-202 (May 1999): 399–401. http://dx.doi.org/10.1016/s0022-0248(98)01361-x.

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14

Dunleavy, C. S., I. O. Golosnoy, J. A. Curran, and T. W. Clyne. "Characterisation of discharge events during plasma electrolytic oxidation." Surface and Coatings Technology 203, no. 22 (August 2009): 3410–19. http://dx.doi.org/10.1016/j.surfcoat.2009.05.004.

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15

Parry, K. L., A. G. Shard, R. D. Short, R. G. White, J. D. Whittle, and A. Wright. "ARXPS characterisation of plasma polymerised surface chemical gradients." Surface and Interface Analysis 38, no. 11 (2006): 1497–504. http://dx.doi.org/10.1002/sia.2400.

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16

Almazán-Almazán, M. Carmen, J. I. Paredes, M. Pérez-Mendoza, M. Domingo-García, F. J. López-Garzón, A. Martínez-Alonso, and J. M. D. Tascón. "Surface characterisation of plasma-modified poly(ethylene terephthalate)." Journal of Colloid and Interface Science 293, no. 2 (January 2006): 353–63. http://dx.doi.org/10.1016/j.jcis.2005.06.073.

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17

REID, S., S. YANG, R. BROWN, K. KABANI, E. AKLILU, P. J. HO, N. WOODLAND, and D. JOSHUA. "Characterisation and relevance of CD138-negative plasma cells in plasma cell myeloma." International Journal of Laboratory Hematology 32, no. 6p1 (February 25, 2010): e190-e196. http://dx.doi.org/10.1111/j.1751-553x.2010.01222.x.

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18

Alnaqi, Abdulwahab A., Shahriar Kosarieh, David C. Barton, Peter C. Brooks, and Suman Shrestha. "Material characterisation of lightweight disc brake rotors." Proceedings of the Institution of Mechanical Engineers, Part L: Journal of Materials: Design and Applications 232, no. 7 (March 14, 2016): 555–65. http://dx.doi.org/10.1177/1464420716638683.

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Alumina coated lightweight brake rotors were investigated to evaluate the effect of coating properties on their friction performance and thermal durability. An alumina ceramic coating on AA6082 aluminium alloy (Al-Alloy) and on 6061/40SiC aluminium metal matrix composite (Al-MMC) prepared by plasma electrolytic oxidation was studied using a programme of brake dynamometer and material characterisation tests. The results showed that the plasma electrolytic oxidation alumina layer adhered well to the Al-alloy substrate and was more uniform and durable when compared to that on the aluminium metal matrix composite. The plasma electrolytic oxidation layer significantly improved the hardness of the rotor surface for both Al-alloy and aluminium metal matrix composite substrate. The coated Al-alloy disc brake rotor was demonstrated to give good thermal and friction performance up to high rubbing surface temperatures of the order of 550 ℃, but the rotor eventually failed due to temperature build-up at a critical location.

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19

Bang, Angela S., Steven G. Soule, Tim G. Yandle, A. Mark Richards, and Chris J. Pemberton. "Characterisation of proghrelin peptides in mammalian tissue and plasma." Journal of Endocrinology 192, no. 2 (February 2007): 313–23. http://dx.doi.org/10.1677/joe-06-0021.

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Ghrelin is a 28 amino acid stomach peptide, derived from proghrelin(1–94), that stimulates GH release, appetite and adipose deposition. Recently, a peptide derived from proghrelin(53–75) – also known as obestatin – has been reported to be a physiological antagonist of ghrelin in the rat. Using four specific RIAs, we provide the first characterisation of proghrelin(1–94) peptides in human plasma, their modulation by metabolic manipulation and their distribution in mammalian tissues. ghrelin(1–28) immunoreactivity (IR) in human plasma and rat plasma/stomach consisted of major des-octanoyl and minor octanoylated forms, as determined by HPLC/RIA. Human plasma ghrelin(1–28) IR was significantly suppressed by food intake, oral glucose and 1 mg s.c. glucagon administration. ghrelin(1–28) IR and proghrelin(29–94) IR peptide distributions in the rat indicated that the stomach and gastrointestinal tract contain the highest amounts of the peptides. Human and rat plasma and rat stomach extracts contained a major IR peak of proghrelin(29–94)-like peptide as determined by HPLC/RIA, whereas no obestatin IR was observed. Human plasma proghrelin(29–94)-like IR positively correlated with ghrelin(1–28) IR, was significantly suppressed by food intake and oral glucose and shared with ghrelin(1–28) IR a negative correlation with body mass index. We found no evidence for the existence of obestatin as a unique, endogenous peptide. Rather, our data suggest that circulating and stored peptides derived from the carboxyl terminal of proghrelin (C-ghrelin) are consistent in length with proghrelin(29–94) and respond to metabolic manipulation, at least in man, in similar fashion to ghrelin(1–28).

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20

Costa, G., M. P. Anania, S. Arjmand, A. Biagioni, M. Del Franco, M. Del Giorno, M. Galletti, et al. "Characterisation and optimisation of targets for plasma wakefield acceleration at SPARC_LAB." Plasma Physics and Controlled Fusion 64, no. 4 (March 3, 2022): 044012. http://dx.doi.org/10.1088/1361-6587/ac5477.

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Abstract One of the most important features of plasma-based accelerators is their compactness because plasma modules can have dimensions of the order of mm cm − 1 , providing very high-accelerating fields up to hundreds of GV m − 1 . The main challenge regarding this type of acceleration lies in controlling and characterising the plasma itself, which then determines its synchronisation with the particle beam to be accelerated in an external injection stage in the laser wakefield acceleration (LWFA) scheme. This issue has a major influence on the quality of the accelerated bunches. In this work, a complete characterisation and optimisation of plasma targets available at the SPARC_LAB laboratories is presented. Two plasma-based devices are considered: supersonic nozzles for experiments adopting the self-injection scheme of laser wakefield acceleration and plasma capillary discharge for both particle and laser-driven experiments. In the second case, a wide range of plasma channels, gas injection geometries and discharge voltages were extensively investigated as well as studies of the plasma plumes exiting the channels, to control the plasma density ramps. Plasma density measurements were carried out for all the different designed plasma channels using interferometric methods in the case of gas jets, spectroscopic methods in the case of capillaries.

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21

Jaglic, Z., Z. Kucerova, K. Nedbalcova, P. Kulich, and P. Alexa. "Characterisation of Pasteurella multocida isolated from rabbits in the CzechRepublic." Veterinární Medicína 51, No. 5 (March 20, 2012): 278–87. http://dx.doi.org/10.17221/5547-vetmed.

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Twenty seven Pasteurella multocida rabbit isolates were characterised by pulsed-field gel electrophoresis (PFGE) generated by restriction endonuclease ApaI, and examined for the presence of capsule and fimbriae, and tested of haemolytic activity and endotoxin release in presence of rabbit plasma. Among the isolates a high number (n = 20) of different PFGE types was observed. All isolates were found to be encapsulated and four of them also possessed fimbriae. Although the presence of the aphA gene, conferring a haemolytic phenotype for Escherichia coli under anaerobic conditions, was indicated in all of the isolates by PCR, clear haemolytic activity was observed in only one isolate when grown anaerobically. All of the isolates grew easily in the presence of rabbit plasma which showed a high capacity to bind the released endotoxin.

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22

Selvaraj Uthra, Ramachandiran Sivaramakrishnan, Thirukumar Sangeshwari, Ganapathy Sivaranjani, and Shankar Kanchana and Muthuvel Arumugam. "Characterisation and Biological Activity of Stingray Venom (HimanturaImbricata)." International Journal for Modern Trends in Science and Technology 7, no. 03 (April 10, 2021): 35–43. http://dx.doi.org/10.46501/ijmtst0703006.

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Stingrays envenomation in humanswere the common accident in the marine and freshwater ecosystem. To determine such effect species Himantura imbricata have been used to elucidate Hemolytic activity, Plasma Coagulation, Fibrin coagulation, Fibrinolytic activity, Activated Partial Thromboplastin Time (APTT),and Prothrombin Time (PT) effects were studied. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Amino acid analysis by RP-HPLC, FTIR spectral analysis and SEM were carried for characterization studies. The results show the of plasma coagulation, fibrin coagulation, of this stingray venom delays the coagulation of citrated plasma. APTT and PT results showed intrinsic and extrinsic coagulation factors that were responsible in time delay when compared with the control. Moreover, these biological experiments and characterization studies aided in understanding the envenoming factors and these results might base the development of treatments for complex diseases.

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23

Warwick, P. E., B. C. Russell, I. W. Croudace, and Ž. Zacharauskas. "Evaluation of inductively coupled plasma tandem mass spectrometry for radionuclide assay in nuclear waste characterisation." Journal of Analytical Atomic Spectrometry 34, no. 9 (2019): 1810–21. http://dx.doi.org/10.1039/c8ja00411k.

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24

Llorca-Isern, Núria, and Gemma Bertran-Vidal. "Characterisation of the Densification Mechanism of Plasma Sprayed Cordierite." Materials Science Forum 654-656 (June 2010): 2374–77. http://dx.doi.org/10.4028/www.scientific.net/msf.654-656.2374.

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Free-standing samples of plasma sprayed cordierite with a high level of porosity were heat treated at different temperatures and for different holding times in order to study the densification process. Optimisation of a new characterisation methodology for the interconnected defects network in the specimens of plasma sprayed cordierite based on fluorescence confocal scanning laser microscopy (CSLM) information was the main objective of the present work. Optical, electron and confocal microscopes were use to determine percentage, distribution and morphology of the defects in the specimens. Fractal geometry combined to the CSLM images was useful for evaluating structural characteristics, complexity and level of connectivity of the defects network of these samples. All this information allowed studying the level of densification accomplished by the samples under the different thermal treatments conditions. After the evaluation of the results produced, this methodology can be validated for other applications or processes such as conventional sintering, etc. Results showed low percentage of porosity with dispersion the latter related to the degree of anisotropy of the samples. However an increase of porosity with the crystallisation was also observed.

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25

Härting, M., A. Hempel, M. Hempel, R. Bucher, and D. T. Britton. "Defect Structural Characterisation of Plasma-Sprayed Bioactive Ceramic Coatings." Materials Science Forum 363-365 (April 2001): 502–4. http://dx.doi.org/10.4028/www.scientific.net/msf.363-365.502.

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26

Vadgama, Pankaj. "Urea pH electrodes: characterisation and optimisation for plasma measurements." Analyst 111, no. 8 (1986): 875. http://dx.doi.org/10.1039/an9861100875.

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27

Bartuli, C., T. Valente, E. Bemporad, and F. Carassiti. "Rapid solidification of plasma sprayed advanced materials: nanostructure characterisation." International Journal of Materials and Product Technology 20, no. 5/6 (2004): 377. http://dx.doi.org/10.1504/ijmpt.2004.004776.

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28

Tian, Jing, Pu Tang, Ping Ma, Lutong Li, Ruiming Li, Ziyuan He, and Bo Chen. "Single-frequency reflection characterisation of shock tube excited plasma." AIP Advances 7, no. 8 (August 2017): 085115. http://dx.doi.org/10.1063/1.4996450.

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29

Tušek, Lidija, Mirko Nitschke, Carsten Werner, Karin Stana-Kleinschek, and Volker Ribitsch. "Surface characterisation of NH3 plasma treated polyamide 6 foils." Colloids and Surfaces A: Physicochemical and Engineering Aspects 195, no. 1-3 (December 2001): 81–95. http://dx.doi.org/10.1016/s0927-7757(01)00831-7.

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30

Ben Amor, S., G. Baud, M. Jacquet, G. Nansé, P. Fioux, and M. Nardin. "XPS characterisation of plasma-treated and alumina-coated PMMA." Applied Surface Science 153, no. 2-3 (January 2000): 172–83. http://dx.doi.org/10.1016/s0169-4332(99)00354-2.

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31

Batty, I., M. Cooke, and V. J. Law. "Harmonic characterisation of a plasma-tool using a diplexer." Vacuum 52, no. 4 (April 1999): 509–14. http://dx.doi.org/10.1016/s0042-207x(98)00341-8.

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32

Yavuz, H., Z. Rzaev, and N. Dilsiz. "Characterisation of flame retardant plasma polymer deposited BOPP film." Plastics, Rubber and Composites 37, no. 5-6 (June 2008): 268–75. http://dx.doi.org/10.1179/174328908x309385.

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33

Zong, Haohua, and Marios Kotsonis. "Characterisation of plasma synthetic jet actuators in quiescent flow." Journal of Physics D: Applied Physics 49, no. 33 (July 25, 2016): 335202. http://dx.doi.org/10.1088/0022-3727/49/33/335202.

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34

Feng, X., J. H. Sui, W. Cai, and X. J. Mi. "Characterisation of rare earth assisted plasma carbonitrided TiNi alloys." Surface Engineering 28, no. 8 (September 2012): 636–38. http://dx.doi.org/10.1179/1743294412y.0000000028.

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35

Jonkers, J., J. M. de Regt, B. van der Sijde, and J. A. M. van der Mullen. "Spectroscopic Techniques for the Characterisation of Spectrochemical Plasma Sources." Physica Scripta T83, no. 1 (1999): 146. http://dx.doi.org/10.1238/physica.topical.083a00146.

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36

Bertóti, I., M. Mohai, J. L. Sullivan, and S. O. Saied. "Surface characterisation of plasma-nitrided titanium: an XPS study." Applied Surface Science 84, no. 4 (April 1995): 357–71. http://dx.doi.org/10.1016/0169-4332(94)00545-1.

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37

Kan, C. W., and C. W. M. Yuen. "Surface characterisation of low temperature plasma-treated wool fibre." Journal of Materials Processing Technology 178, no. 1-3 (September 2006): 52–60. http://dx.doi.org/10.1016/j.jmatprotec.2005.11.018.

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38

Wright, Alexander, Matteo Taglioli, Faraz Montazersadgh, Alex Shaw, Felipe Iza, and H. C. Hemaka Bandulasena. "Microbubble-enhanced DBD plasma reactor: Design, characterisation and modelling." Chemical Engineering Research and Design 144 (April 2019): 159–73. http://dx.doi.org/10.1016/j.cherd.2019.01.030.

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39

Almhanna, Hazem. "Characterisation of ADAMs (protein)of bovine seminal plasma by Mass Spectrometry." Kufa Journal For Veterinary Medical Sciences 10, no. 2 (December 31, 2019): 1–7. http://dx.doi.org/10.36326/kjvs/2019/v10i23308.

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This study has been designed to analyse the secreted and soluble proteins in bovine seminal plasma by mass spectrometry. The majority of these proteins are produced by accessory glands, and partially by testis, epididymis, ductus deferens and vas deferens of male reproductive tract. Seminal plasma of bovine was collected freshly and isolated after centrifuged and removed the sperm. Non boiled and boiled seminal plasma lysate were run to identify and detect the total proteins, as well as the individual single member of ADAMs protein is determined. The non-boiled lysate of seminal plasma was displayed diversity of proteins more than boiled sample lysate of seminal plasma. However, boiled and non-boiled seminal plasma have distinguished several types of ADAMs protein which are included: ADAM10, ADAM9, ADAM7, ADAM15 in non-boiled samples, while boiled lysate of bovine seminal plasma was displayed ADAM10, ADAM9, ADAM28, and ADAM22. Our finding concluded that bovine seminal plasma is very rich in different types of soluble, cleaved and shed proteins such ADAMs protein which could potentially have a biological and physiological role in protection and interactions of sperm during motility inside female reproductive tract, as it might support it to fertilise the ovum. This protection might be via immunosuppression behaviour or by block specific receptors in female reproductive tract for enhancing sperm motility, and avoid sperm the singling response of immunity.

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40

Nestorow, Stephanie A., Tim R. Dafforn, and Verna Frasca. "Biophysical characterisation of SMALPs." Biochemical Society Transactions 49, no. 5 (October 13, 2021): 2037–50. http://dx.doi.org/10.1042/bst20201088.

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Membrane proteins such as receptors, ion channels and transport proteins are important drug targets. The structure-based study of membrane proteins is challenging, especially when the target protein contains both soluble and insoluble domains. Most membrane proteins are insoluble in aqueous solvent and embedded in the plasma membrane lipid bilayer, which significantly complicates biophysical studies. Poly(styrene-co-maleic acid) (SMA) and other polymer derivatives are increasingly common solubilisation agents, used to isolate membrane proteins stabilised in their native lipid environment in the total absence of detergent. Since the initial report of SMA-mediated solubilisation, and the formation of SMA lipid particles (SMALPs), this technique can directly isolate therapeutic targets from biological membranes, including G-protein coupled receptors (GPCRs). SMA now allows biophysical and structural analyses of membrane proteins in solution that was not previously possible. Here, we critically review several existing biophysical techniques compatible with SMALPs, with a focus on hydrodynamic analysis, microcalorimetric analysis and optical spectroscopic techniques.

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41

Daykin-Iliopoulos, Alexander, Franco Bosi, Fabio Coccaro, Mirko Magarotto, Athanasios Papadimopoulos, Paola De Carlo, Cristian Dobranszki, Igor Golosnoy, and Steve Gabriel. "Characterisation of a thermionic plasma source apparatus for high-density gaseous plasma antenna applications." Plasma Sources Science and Technology 29, no. 11 (November 18, 2020): 115002. http://dx.doi.org/10.1088/1361-6595/abb21a.

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42

Roychowdhury, P., A. S. Paithankar, S. K. Iyyengar, and V. K. Rohatgi. "Characterisation of target plasma required for REB-plasma interaction studies using cylindrical Langmuir probes." Plasma Physics and Controlled Fusion 29, no. 5 (May 1, 1987): 651–59. http://dx.doi.org/10.1088/0741-3335/29/5/006.

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43

Wu, Jui-Te, Pei-Shiue Tsai, Shuang-Lin Lee, and Feng-Pang Cheng. "Characterisation of the progesterone receptor on canine spermatozoa." Reproduction, Fertility and Development 17, no. 7 (2005): 733. http://dx.doi.org/10.1071/rd05074.

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The present study was conducted to characterise and localise the progesterone receptor (PR) on canine spermatozoa. Using a progesterone–bovine serum albumin–fluorescein isothiocyanate conjugate (PBF) and different monoclonal antibodies (C262 and NCL-PGR against the steroid binding domain and N-terminus of intracellular PR, respectively, and h151 against the hinge domain of the intracellular oestrogen receptor), the PR was identified on the plasma membrane over the acrosomal region. Two proteins (54 kDa and 65 kDa) were detected by recognition of the three monoclonal antibodies using Western blotting. PBF labelling was observed in the majority of cauda epididymal spermatozoa (63 ± 4%), but this labelling was markedly reduced (33 ± 17%) after the addition of canine seminal plasma. Over a 7-h capacitation, the proportion of ejaculated spermatozoa exhibiting PBF labelling (indicating the presence of the PR) increased from 18 ± 10% (onset) to 59 ± 7% by 5 h, where it plateaued. Progesterone (P4) induced the acrosome reaction (AR) in a dose-dependent manner (0, 0.1, 1 and 10 µg/mL P4 corresponding to 10 ± 5%, 16 ± 9%, 23 ± 7% and 30 ± 7%). Pre-treatment of capacitated spermatozoa with canine seminal plasma reduced the incidence of the P4-induced AR (12 ± 5%). In addition, treatment with the monoclonal antibodies significantly reduced the incidence of the P4-induced AR (10 µg/mL) in capacitated ejaculated spermatozoa from 19 ± 6% to 11 ± 4% (h151, 1 : 10) and 12 ± 6% (C262, 1 : 10), respectively. A typical Scatchard plot revealed one binding with high affinity and low capacity, and another binding with low affinity and high capacity, suggesting at least two different characteristic PR. Taken together, these results demonstrate that P4 induced the AR in a dose-dependent manner via functional transmembranal receptors in the acrosomal region of the canine sperm plasma membrane. The characteristics of this membrane receptor seem similar to those of other mammalian spermatozoa, and it shows structural homology to the intracellular PR.

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44

Murtagh, M., S. Hildebrandt, P. A. F. Herbert, G. M. O'Connor, and G. M. Crean. "Optical and Electrical Characterisation of He Plasma Sputtered n-GaAs." Materials Science Forum 196-201 (November 1995): 1961–66. http://dx.doi.org/10.4028/www.scientific.net/msf.196-201.1961.

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45

Henry, Anne, Bo Monemar, J. Lennart Lindström, T. D. Bestwick, and G. S. Oehrlein. "Photoluminescence Characterisation of the Silicon Surface Exposed to Plasma Treatment." Materials Science Forum 83-87 (January 1992): 1445–50. http://dx.doi.org/10.4028/www.scientific.net/msf.83-87.1445.

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46

Rodriguez, J., V. Boulo, E. Mialhe, and E. Bachere. "Characterisation of shrimp haemocytes and plasma components by monoclonal antibodies." Journal of Cell Science 108, no. 3 (March 1, 1995): 1043–50. http://dx.doi.org/10.1242/jcs.108.3.1043.

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Various haemolymph components of the shrimp Penaeus japonicus were identified and characterised by monoclonal antibodies. Three groups of monoclonal antibodies were raised. Their reactivity to haemocyte types and/or secreted molecules was determined by immunofluorescence and the molecular masses of the antigens were analysed by western-blotting. A 170 kDa protein, in reducing conditions, was recognized by four panhaemocytic monoclonal antibodies from group 1. This protein was present both in the plasma and in the haemocytes from which it appears to be secreted. The shrimp haemocytes were separated by isopycnic centrifugation on a Percoll gradient and the different subpopulations were antigenically analysed using the two monoclonal antibodies, 40E2-2A and 40E10-2B, from group 2. The granular cells were labelled by 40E2-2A which was specific for a protein of 142 kDa also present in plasma. By comparison, the 40E10-2B monoclonal antibody was assumed to be the marker for small hyaline and semigranular cells since the granular ones were not labelled. Moreover, the antigen immunoprecipitated by this monoclonal antibody was shown to have different molecular masses of 250, 150, 66 and 27 kDa under nonreducing conditions. It appeared to be secreted by the haemocytes. Some plasma proteins were recognized by the third group of monoclonal antibodies. The antibodies, designated 41D11-3A, 42C11-3B and 42E8-3C, all immunoprecipitated a protein with an apparent molecular mass of 180 kDa under reduced conditions. The 44E6-3D antibody was specific for a 75 kDa protein under reduced conditions and was shown to be immunoreactive against P. japonicus haemocyanin extract.(ABSTRACT TRUNCATED AT 250 WORDS)

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47

Kelliher, S., H. Macleod, L. Weiss, P. B. Szklanna, S. Gamba, F. Ni Ainle, F. Schieppati, et al. "PO-08: Characterisation of plasma extracellular vesicles in myeloproliferative neoplasms." Thrombosis Research 213 (May 2022): S14—S15. http://dx.doi.org/10.1016/s0049-3848(22)00196-7.

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Castillo-Mejía, F., I. Gamboa-de Buen, J. J. E. Herrera-Velázquez, and José Rangel-Gutiérrez. "Neutron emission characterisation at the FN-II Dense Plasma Focus." Journal of Physics: Conference Series 511 (May 7, 2014): 012021. http://dx.doi.org/10.1088/1742-6596/511/1/012021.

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WATSON, T. D. G., LYNN BURNS, S. LOVE, C. J. PACKARD, and J. SHEPHERD. "The isolation, characterisation and quantification of the equine plasma lipoproteins." Equine Veterinary Journal 23, no. 5 (September 1991): 353–59. http://dx.doi.org/10.1111/j.2042-3306.1991.tb03737.x.

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Noh, B. I., and S. B. Jung. "Characterisation of FR-4 substrate with various plasma treatment conditions." Materials Science and Technology 23, no. 7 (July 2007): 792–98. http://dx.doi.org/10.1179/174328407x168874.

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Journal articles on the topic 'Plasma characterisation'

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