Dissertations / Theses on the topic 'Plants Metabolism'
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Garlick, Andrew P. "Carbohydrate metabolism during oxidative stress in plants." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270014.
Full textTuran, Y. "Pyrimidine primary and secondary metabolism in plants." Thesis, Swansea University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.639271.
Full textBlume, Christian [Verfasser]. "Glycolate and glyoxylate metabolism in higher plants : how natural and artificial pathways contribute to plant metabolism / Christian Blume." Hannover : Technische Informationsbibliothek (TIB), 2013. http://d-nb.info/1130810666/34.
Full textBasson, Carin Elizabeth. "Analysis of intermediate carbon metabolism in strawberry plants." Thesis, Link to the online version, 2008. http://hdl.handle.net/10019/1907.
Full textSachan, Nita. "Identification of signaling factors involved in the regulation of alkaloid metabolism in N.tabacum." Lexington, Ky. : [University of Kentucky Libraries], 2004. http://lib.uky.edu/ETD/ukyplph2004d00179/NS%5FDiss.pdf.
Full textTitle from document title page (viewed Jan. 7, 2005). Document formatted into pages; contains x, 127p. : ill. Includes abstract and vita. Includes bibliographical references (p. 118-126).
Tront, Jacqueline Marie. "Plant Activity and Organic Contaminant Processing by Aquatic Plants." Diss., Georgia Institute of Technology, 2004. http://hdl.handle.net/1853/5234.
Full textHardy, D. J. "Studies of tocopherol and phospholipid metabolism in plants." Thesis, Bangor University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384175.
Full textMasakapalli, Shyam Kumar. "Network flux analysis of central metabolism in plants." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:ac8b3836-9ab7-4060-b50a-df8aaa0e4ba5.
Full textMasterson, Christine. "Carnitine and fatty acid metabolism in higher plants." Thesis, University of Newcastle Upon Tyne, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254030.
Full textFoley, A. A. "Metabolism and function of membrane lipids in plants." Thesis, Bucks New University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356208.
Full textCowan, Ashton Keith. "The metabolism of abscisic acid in higher plant tissues." Thesis, Rhodes University, 1989. http://hdl.handle.net/10962/d1002024.
Full textHoppen, Carolina. "Efeitos do estresse biótico na expressão de terpenos em plantas: Varronia curassavica Jacq. and Pistacia palaestina Boiss." Universidade Tecnológica Federal do Paraná, 2018. http://repositorio.utfpr.edu.br/jspui/handle/1/3260.
Full textOs terpenos constituem a classe de produtos naturais com maior diversidade química e estrutural, estando associados ao metabolismo das plantas e às interações destas com outros organismos. Estes compostos, as enzimas que os sintetizam e as plantas que os produzem são amplamente estudados em diferentes aspectos. Para melhor compreensão da expressão de terpenos em plantas sob estresse biótico, as espécies Varronia curassavica e Pistacia palaestina foram estudadas neste trabalho. Folhas de V. curassavica contém óleo essencial rico em sesquiterpenos com propriedades anti-inflamatórias, especialmente α-humuleno and β-cariofileno. O objetivo deste estudo foi avaliar as respostas do seu metabolismo em função da aplicação de dois eliciadores naturais no conteúdo de sesquiterpenos de V. curassavica. Para isso, as plantas receberam a aplicação de acibenzolar-S-metil (500 mg L-1), 1,6 β-D-glucano obtido a partir de Lasiodiplodia theobromae (50 mg L-1) e água destilada como controle, sendo realizada as avaliações de trocas gasosas, atividade das enzimas fenilalanina amônia-liase, superóxido dismutase, peroxidase e catalase, bem como a análise química do óleo essencial. Acibenzolar-S-metílico reduziu significativamente a taxa líquida de assimilação de carbono e a concentração intercelular de CO2, enquanto que 1,6 β-D-glucano reduziu significativamente apenas a concentração intercelular de CO2. O maior rendimento de óleo essencial (0.819%) foi obtido em plantas eliciadas por 1,6 β-D-glucano. As proporções relativas e a quantidade de α-humuleno e β-cariofileno não diferiram entre os tratamentos, porém os eliciadores aumentaram significativamente a atividade da enzima guaiacol peroxidase. Os terpenos estão presentes nas folhas e galhas de Pistacia palaestina. Apesar do mecanismo de desenvolvimento das galhas ainda não ter sido completamente elucidado, sabe-se que espécies de afídeos como Baizongia pistaciae L. manipulam anatomicamente, fisiologicamente e quimicamente as plantas hospedeiras em seu benefício. Por este motivo, o isolamento e a caracterização funcional dos genes que codificam terpeno sintases em galhas induzidas por B. pistaciae, bem como sua expressão relativa por RT-qPCR em folhas e galhas de P. palaestina foram os objetivos deste trabalho. A expressão heteróloga foi realizada em E. coli pLYS-BL21, sendo as reações enzimáticas feitas com proteínas purificadas e usando geranil difosfato (GPP) ou farnesil difosfato (FPP) para testar a atividade das enzimas como mono- e sesquiterpeno sintases, respectivamente. Para o experimento de RT-qPCR, foi selecionado um gene referência entre actina, ciclofilina, fosfoglicerato quinase, RNA polimerase II, α-tubulina e ubiquitina. Em seguida, realizaram-se reações com as terpeno sintases para avaliar as diferenças nos níveis de expressão em folhas não colonizadas e galhas. Foram isoladas e caracterizadas duas monoterpeno sintases (PpTPS281 e PpTPS809) e uma sesquiterpeno sintase (PpTPS232) em P. palaestina. PpTPS281 produziu exclusivamente D-limoneno a partir de GPP, enquanto PpTPS809 produziu vários monoterpenos a partir de GPP e PpTPS232 catalisou a formação de diferentes sesquiterpenos a partir de FPP. Os resultados da RT-qPCR mostraram que o gene actina é o mais apropriado para ser usado na comparação de expressão de genes de folhas não colonizadas e galhas induzidas por B. pistaciae. Os níveis de expressão dos três genes foram significativamente aumentados em galhas (de 2,21- a 96.5-vezes), quando comparados com folhas.
Terpenes are a large and diverse class of natural products, being associated with plant metabolism and interactions with other organisms. Nowadays compounds and enzymes of the terpenes pathway in plants are widely studied in different aspects. Varronia curassavica and Pistacia palaestina were the selected species to study the biotic stress effects on terpenoids expressions. Leaves of V. curassavica are the commercial source α-humulene and β-caryophyllene, sesquiterpenes with anti-inflammatory properties. The objective of this study was to evaluate the effect of two natural elicitors on the sesquiterpene content of V. curassavica. Thus, field grown plants received the application of acibenzolar-S-methyl (500 mg L-1), 1,6 -D-glucan obtained from Lasiodiplodia theobromae (50 mg L-1) and distilled water as a control. Gas exchange rate, terpene enzymes such as phenylalanine ammonia-lyase, superoxide dismutase, guaiacol peroxidase and catalase activity and essential oil content in leaves were measured. Acibenzolar-S-methyl reduced significantly the net carbon assimilation rate and the intercellular CO2 concentration, while1,6 -D-glucan reduced significantly only the intercellular CO2 concentration. The highest essential oil yield (0.819%) was obtained in plants elicited with 1,6 -D-glucan. The content of α-humulene and β-caryophyllene did not differ among treatments however the elicitors provided a significant increase in guaiacol peroxidase activity. Terpenes are present in Pistacia palaestina in leaves and in horn-shaped galls. The mechanism of gall development remains unknown, however it is clear Baizongia pistaciae L., an aphid species, manipulates their hosts anatomy, physiology, and chemistry for their own benefit. To isolate and functional characterize terpene synthase genes from galls induced by B. pistaciae as well as their gene relative expression by RT-qPCR in leaves and galls of P. palestina were the aims of this study. The heterologous expression was performed in E. coli pLYS-BL21 cells, being enzymatic assay reactions made with the purified proteins using geranyl diphosphate (GPP) or farnesyl diphosphate (FPP) to test for possible mono- and sesquiterpene synthase activity, respectively. For relative real-time PCR, it was selected an appropriate reference gene between actin, cyclophilin, phosphoglycerate kinase, RNA polymerase II, α-tubulin and ubiquitin. After selection, it was performed reactions with terpene synthases genes to evaluate differences in expression levels in P. palaestina non-colonized leaves and galls. Two monoterpene synthases (PpTPS281 and PpTPS809) and one sesquiterpene synthase (PpTPS232) were isolated and characterized in P. palaestina. PpTPS281 produced exclusively D-limonene from GPP, while PpTPS809 produced several monoterpenes from GPP and PpTPS232 catalyzed the formation of different sesquiterpenes from FPP. Real-time PCR results showed that actin is the most proper gene to be used for genes expression studies between non-colonized P. palaestina leaves and galls induced by B. pistaciae. The levels of expression of the genes were significantly upregulated in galls (from 2,21- to 96.5-fold) when compared to leaves.
Laikowski, Manuela Merlin. "Avaliação dos principais metabólicos secundários por espectrometria de massas e atividade hipoglicêmica de Salacia impressifolia Miers A. C. Smith." reponame:Repositório Institucional da UCS, 2015. https://repositorio.ucs.br/handle/11338/932.
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The use of plants in various ailments healing is traditionally known and used for centuries by mankind. Plant cells produce a large variety of chemical compounds between the primary and secondary metabolism. The secondary metabolism products are of special interest, as they are responsible for several biological activities. Ethnobotanic information indicates that more than 800 plants are used as traditional remedies in the treatment of diabetes. Many species of Salacia genus have been described with antidiabetic activity, including Salacia impressifolia. This specie belongs to Celastraceae family, popularly known as miraruíra in Brazil. This work aimed to develop the chemical composition of this specie through stem and stem bark extracts. Chemical analysis of the extracts was carried out by high resolution mass spectrometry (HRMS). In these extracts, 32 compounds were identified, previously described for the same genus. Among them, were identified kotalanol, salacinol and mangiferina, with hypoglycemic action previously described by others authors. For in vivo analysis of hypoglycemic activity, it was performed the hydroalcoholic stem extract in streptozotocin induced diabetics rats. The extract presented similar action to control drug glibenclamide. It was also assessed the lipid profile (triglycerides, total cholesterol and HDL) and liver enzymes AST and ALT, and it was observed an increase in HDL level.
Champanis, Reinette. "Aspects of sucrose metabolism in transgenic tobacco." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/49854.
Full textENGLISH ABSTRACT: In most plants the efficiency of sucrose production and the systemic distribution thereof are the major determinants of growth, development and yield. The factors governing sugar partitioning co-ordinate its distribution in response to intrinsic and environmental signals. These factors include sugar transporters and invertases as well as metabolites, including sucrose and glucose, which function as signalling molecules to modulate gene expression. The genetic transformation of plants and the subsequent development of transgenic lines with disturbed sugar metabolism have made an unprecedented impact on the study of sugar translocation and -partitioning. For instance, the transformation of plants with a yeast-derived invertase targeted to different subcellular compartments has led to the elucidation of several key aspects of sugar metabolism, including phloem loading mechanisms, the regulation of photosynthesis by sugars, the importance of sugar-metabolism compartmentation with regards to sucrose biosynthesis, storage and distribution, as well as the role of cell-wall invertase in phloem unloading and sink strength. In this study, a similar strategy of transgenic plant analysis was employed to expand our insight into the regulation of sugar partitioning. The yeast-invertase Suc2 gene, from Saccharomyces cere visiae , was overexpressed in either the cytosol, vacuole or apoplast of transgenic tobacco plants. These transgenic lines displayed varying increases in invertase activity, altered sugar levels and consequently disturbed sink-source interactions and sugar partitioning. Transgenic lines overproducing the yeast-derived invertase in either the vacuole (Vac-Inv) or apoplast (Apo-Inv) were utilised to analyse the effect of the altered sugar levels in sink and source organs on the expression of sugar transporters, as well as the endogenous cell wall invertase and inhibitors in these plants. Transcript levels of the sucrose transporter NtSUT1 and hexose transporter NtMST1 encoding genes increased significantly in the source leaves and roots of Vac-Inv lines, whereas increased NtMst1 transcript levels were also detected in the roots of Apo-Inv lines. The increased mRNA levels could be correlated to the altered invertase activities and sugar levels in these tissues. It is concluded that NtSUT1 and NtMST1 are differentially regulated by sucrose and/or hexose content on a transcriptional level. Furthermore, the regulatory effect of the altered sugar levels on transporter expression depended on the subcellular compartment in which the yeast invertase was expressed. It would seem that the subcellular compartmentation of sugar metabolism is also fundamental to the regulation of sugar partitioning. The transcription levels of the endogenous cell wall invertase (CWt) and cell wall invertase inhibitor (Cwi-Inh) genes were examined in the various tissues of Apo-Inv and Vac-Inv lines at both the vegetative and flowering growth stages. In comparison with the control lines, the various tissues of the Apo-Inv and Vac-Inv lines displayed altered Cwi and Cwi-Inh expression levels, depending on the sink-source status and growth stage. However, no obvious correlation between the Cwi and Cwi-Inh expression levels and soluble sugar content of these tissues was found. It is suggested that the post-transcriptional and post-translation control of these proteins by sugars might play an important role in their regulation. Analysis of the Cwi:Cwi-lnh mRNA ratio and growth observations of the various tissues of control as well as Apo-Inv and Vac-Inv lines indicated that this transcription ratio could be an accurate indicator of the sink strength of sink organs. In addition, the influence of sink-source interactions on sugar partitioning was investigated. Reciprocal grafting between Apo-Inv and control lines resulted in scions with an altered sucrose metabolism in either the sink or source organs. These scions were subjected to biomass distribution, soluble sugar quantification and C4C]- radiolabelling experiments. The latter revealed an unaltered state of sugar partitioning from the above-ground tissues of the Apo/GUS scions and a significant shift in sugar partitioning towards the roots of the GUS/Apo scions in comparison to the control GUS/GUS scions. Phenotypic changes, opposite to those observed in Apo-Inv lines expressing the heterologous invertase in both sink and source organs, could initially be observed in the GUS/Apo and Apo/GUS scions. However, no significant differences in phenotype or biomass distribution could be observed between the mature GUS/Apo, Apo/GUS and GUS/GUS scions seven weeks postgrafting. This inconsistency between phenotype and sugar partitioning might be explained by an increase in the respiration rate of the tissues as supported by the soluble sugar content. These results highlight the complexity and adaptability of sucrose metabolism and sugar partitioning. In addition, it confirms that sugar partitioning can be modulated by sink-source interactions and emphasise the importance of invertases in the regulation of sugar partitioning through its ability to alter sink strength. This study forms part of the rapidly expanding initiative to unravel the control mechanisms of sugar partitioning. The results obtained in this study confirmed again that the introduction and expression of a single heterologous gene in transgenic plants could provide significant insight into the regulation of this process. It was shown here that the expression of sugar transporters is closely regulated by sugar levels and therefore fulfils a vital function in sugar sensing and consequently the regulation of sugar partitioning. The data presented in this study also demonstrated the intricate and flexible nature of the relationship that exists between sugar metabolism, partitioning and growth phenomena.
AFRIKAANSE OPSOMMING: Die doeltreffendheid van sukroseproduksie, tesame met die sistemiese verspreiding daarvan, is die vernaamste faktore wat die groei, ontwikkeling en opbrengsvermoë van die meeste plante bepaal. Die faktore wat suikerverdeling beheer, funksioneer om suikerverspreiding te koordineer in reaksie op beide inherente- en omgewingsseine. Hierdie faktore sluit suikertransporters en invertases in, asook metaboliete soos sukrose en glukose wat funksioneer as seinmolekule in die modulering van geenuitdrukking. Die genetiese transformasie van plante en die gevolglike daarstelling van transgeniese lyne met veranderde suikermetabolismes het 'n beduidende inwerking op die bestudering van suikervervoer en -verdeling gehad. Byvoorbeeld, die transformasie van plante met 'n gis-invertase geteiken na verskillende sub-sellulêre kompartemente, het tot die toeligting van verskeie aspekte van suikermetabolisme gelei, insluitende dié van floëemladingsmeganismes, die regulering van fotosintese deur suikers, die belang van kompartementalisering ten opsigte van sukrosebiosintese, -opberging en -verspreiding, en die rol van selwand-invertases in floëemontlaaiing en swelgpuntkrag. In hierdie studie is van soortgelyke transgeniese plantontledings gebruik gemaak om 'n dieper insig tot die regulering van suikerverdeling te verkry. Die gis-invertase Suc2 geen, afkomstig van Saccharomyces cerevisiae, is ooruitgedruk in óf die sitosol, vakuool óf apoplastiese ruimte van transgeniese tabakplante. Hierdie transgeniese lyne het wisselende toenames in invertase-aktiwiteite en veranderde suikervlakke getoon, asook gevolglike versteurde bron-swelgpunt interaksies en suikerverdeling. Transgeniese lyne met ooruitdrukking van die gis-invertase in óf die vakuool (Vac-Inv) óf die apoplast (Apo-Inv) is gebruik om die gevolg van die veranderde suikervlakke in bron- en swelgpuntorgane op die uitdrukking van suikertransporters, asook die endogene selwand-invertase en invertase-inhibitor in hierdie plante te bepaal. Transkripsievlakke van die sukrosetransporter NtSut1 en die heksosetransporter, NtMst1, het beduidend toegeneem in die bron-blare en wortels van die Vac-Inv lyne; 'n toename in NtMst1 transkripsievlakke is ook in die wortels van Apo-Inv lyne bevestig. Die toenames in boodskapper RNA kon gekorreleer word met die veranderde invertase-aktiwiteite en suikervlakke in hierdie weefsels. Die gevolgtrekking word gemaak dat NtSUT1 en NtMST1 differensieël gereguleer word op transkripsionele vlak deur die sukrose en/of heksose inhoud van weefsels. Meer nog, die regulerende effek van die veranderde suikervlakke op transporteruitdrukking het afgehang van die subsellulêre kompartement waarin die gis-invertase uitgedruk is. Dit wil dus voorkom dat die subsellulêre kompartementalisering van suikermetabolisme fundamenteel tot die deurgee en waarneming van suikerseine is, met In gevolglike eweneens belangrike rol in die regulering van suikerverdeling. Die transkripsievlakke van beide die endogene selwand-invertase (CWI) en die selwand-invertase-inhibitor (CWI-Inh) enkoderende gene is in verskeie weefsels van die Apo-Inv en Vac-Inv lyne, tydens beide die vegetatiewe- en blomstadia, bestudeer. Die onderskeie weefsels van die Apo-Inv en Vac-Inv lyne het, in vergelyking met die kontrole lyne, veranderde Cwi en Cwi-inh transkripsievlakke getoon wat bepaal is deur bron-swelgpunt status en groeistadium. Geen duidelike korrelasie kon tussen beide Cwi en Cwi-inh uitdrukkingsvlakke en oplosbare suiker inhoud gevind word nie. Daar word voorgestel dat post-transkripsionele en posttranslasionele beheer deur suikers 'n belangrike rol in die regulering van hierdie proteïne speel. Bestudering van die Cwi:Cwi-lnh mRNA verhouding, asook groei verskynsels van die onderskeie weefsels van kontrole en Apo-Inv en Vac-Inv lyne, dui daarop dat hierdie transkripsievlak-verhouding moontlik 'n akkurate aanwyser van die swelgpuntkrag van 'n swelgpuntorgaan kan wees. Voorts is die invloed van bron-swelgpuntorgaan interaksies op suikerverdeling ondersoek. Omgekeerde enting tussen Apo-Inv en kontrole lyne het entlote met gemodifiseerde suikermetabolisme in óf hul bron- óf hul swelgpuntorgane tot gevolg gehad. Hierdie entlote is aan biomassaverspreidings-, oplosbare suiker kwantifisering en C4C]-radiomerking eksperimente onderwerp. Hierdie resultate het gewys dat, in vergelyking met die kontrole (GUS/GUS) ente, daar geen verandering in die status van suikerverdeling vanaf die bogrondse plantdele in die Apo/GUS ente is nie, maar wel 'n beduidende verskuiwing in suikerverdeling na die wortels van die GUS/Apo ente. Fenotipiese veranderinge, wat teenoorgesteld van dié teenwoordig in die Apo- Inv lyne waar die heteroloë invertase in beide bron en swelgpuntorgane uitgedruk word, is aanvanklik in die GUS/Apo en Apo/GUS ente waargeneem. Geen verskille in fenotipe of biomassa-verspreiding kon egter sewe weke na die entings prosedures tussen die GUS/Apo, Apo/GUS and GUS/GUS ente gevind word nie. Dit mag verduidelik word deur 'n moontlike toename in respirasietempo in die betrokke weefsels; die oplosbare suikervlakke wat in die verskillende ente aangeteken is ondersteun dié moontlikheid. Hierdie resultate as geheelonderstreep die kompleksiteit en aanpasbaarheid van suikermetabolisme en -verdeling. Verder bevestig dit dat suikerverdeling beïnvloed kan word deur bron-swelgpunt interaksies, asook die belang van invertases in die regulering van suikerverdeling gegewe die vermoë om swelgpuntkrag te verander. Hierdie studie vorm deel van 'n vinnig groeiende inisiatief om die beheermeganismes van suikerverdeling te ontrafel. Die resultate verkry in hierdie studie bekragtig die belang van rekombinante DNA tegnologie in die bestudering van fundamentele plantprosesse. Die invoeging en uitdrukking van 'n geteikende gisinvertase in transgeniese plante het gelei tot veranderde suikervlakke en bronswelgpunt interaksies in hierdie lyne met die gevolglike ontginning van waardevolle inligting ten opsigte van die regulering van suikerverdeling in reaksie tot interne seine. Daar is aangetoon dat suikertransporters onlosmaakbaar gekoppel is aan die deurgee en waarneming van suikerseine, spesifiek op die vlak van transkripsionele regulering, en dus ook die regulering van suikerverdeling. Voorts wys die resultate op die komplekse en aanpasbare aard van die verhouding wat bestaan tussen suikermetabolisme, -verdeling en groeiverskynsels.
Tetlow, Ian Joseph. "Photosynthetic carbon metabolism in plants infected with biotrophic fungi." Thesis, Bangor University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293196.
Full textSharma, Suraj [Verfasser]. "Mathematical models of glucosinolate metabolism in plants / Suraj Sharma." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2018. http://d-nb.info/1161182772/34.
Full textWilliams, Jane S. "Elemental sulphur metabolism in plants and defence against pathogens." Thesis, University of Bath, 2002. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268744.
Full textNicholson, Tarryn Louise. "Carbon turnover and sucrose metabolism in the culm of transgenic sugarcane producing 1-Kestose." Thesis, Link to the online version, 2007. http://hdl.handle.net/10019/693.
Full textJohansson, Henrik. "Gene regulation of UDP-glucose synthesis and metabolism in plants." Doctoral thesis, Umeå University, Plant Physiology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-93.
Full textPhotosynthesis captures light from the sun and converts it into carbohydrates, which are utilised by almost all living organisms. The conversion between the different forms of carbohydrates is the basis to form almost all biological molecules.
The main intention of this thesis has been to study the role of UDP-glucose in carbohydrate synthesis and metabolism, and in particular the genes that encode UDP-glucose pyrophosphorylase (UGPase) and UDP-glucose dehydrogenase (UGDH) in plants and their regulation. UGPase converts glucose-1-phosphate to UDP-glucose, which can be utilised for sucrose synthesis, or cell wall polysaccharides among others. UGDH converts UDP-glucose to UDP-glucuronate, which is a precursor for hemicellulose and pectin. As model species I have been working with both Arabidopsis thaliana and poplar.
Sequences for two full-length EST clones of Ugp were obtained from both Arabidopsis and poplar, the cDNAs in Arabidopsis correlate with two genes in the Arabidopsis genomic database.
The derived protein sequences are 90-93% identical within each plants species and 80-83% identical between the two species.
Studies on Ugp showed that the expression is up-regulated by Pi-deficiency, sucrose-feeding and by light exposure in Arabidopsis. Studies with Arabidopsis plants with mutations in sugar/ starch- and Pi-content suggested that the Ugp expression is modulated by an interaction of signals derived from Pi-deficiency, sugar content and light/ dark conditions, where the signals act independently or inhibiting each other, depending on conditions. Okadaic acid, a known inhibitor of certain classes of protein phosphatases, prevented the up-regulation of Ugp by Pi-deficiency and sucrose-feeding. In poplar, sucrose also up-regulated the expression of Ugp. When poplar and Arabidopsis were exposed to cold, an increase of Ugp transcript content was detected as well as an increase in UGPase protein and activity. In poplar, Ugp was found to be expressed in all tissues that were examined (differentiating xylem, phloem, apical leaves and young and mature leaves).
By using antisense strategy, Arabidopsis plants that had a decrease in UGPase activity of up to 30% were obtained. In the antisense plants, the soluble carbohydrate content was reduced in the leaves by at least 50%; in addition the starch content decreased. Despite the changes in carbohydrate content, the growth rate of the antisense plants was not changed compared to wild type plants under normal growth conditions. However, in the antisense lines the UGPase activity and protein content in sliliques and roots increased, perhaps reflecting compensatory up-regulation of second Ugp gene. This correlates with a slightly larger molecular mass of UGPase protein in roots and siliques when compared to that in leaves. Maximal photosynthesis rates were similar for both wild type and antisense plants, but the latter had up to 40% lower dark respiration and slightly lower quantum yield than wild type plants.
Two Ugdh cDNAs from poplar and one from Arabidopsis were sequenced. The highest Ugdh expression was found in xylem and younger leaves. Expression data from sugar and osmoticum feeding experiment in poplar suggested that the Ugdh expression is regulated via an osmoticumdependent pathway.
Lundmark, Maria. "Low temperature acclimation in plants : alterations in photosynthetic carbon metabolism." Doctoral thesis, Umeå : Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1333.
Full textQiao, ZhengDao. "Interactions between NO2 absorption and nitrogen metabolism in soyabean plants." Thesis, Qiao, ZhengDao (1998) Interactions between NO2 absorption and nitrogen metabolism in soyabean plants. PhD thesis, Murdoch University, 1998. https://researchrepository.murdoch.edu.au/id/eprint/51679/.
Full textEl-Shora, Hamed Mohammad. "The role of malic enzyme (NADP-ME) in plants." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278257.
Full textRobinson, Charles Kinsman. "Regulation of resource allocation during reproductive growth in Arabidopsis thaliana L. Heynh." Thesis, University of Oxford, 2000. http://ora.ox.ac.uk/objects/uuid:40a22dcc-5ad5-4e8b-af42-55488c912ddb.
Full textLee, Alex Chun Pong. "Dynamics of the plant mitochondrial proteome : towards the understanding of metabolic networks." University of Western Australia. School of Biomedical and Chemical Sciences, 2009. http://theses.library.uwa.edu.au/adt-WU2009.0181.
Full textFredericks, Eugene B. (Eugene Bernard). ""Studies involving alterations of polyamine metabolism in Arabidopsis thaliana"." Monash University, Dept. of Biological Sciences, 2001. http://arrow.monash.edu.au/hdl/1959.1/8432.
Full textKiyota, Eduardo 1977. "Identificação e caracterização da enzima aminoadípico semialdeído desidrogenase em plantas = Identification and characterization of the aminoadipic semialdehyde dehydrogenase enzyme in plants." [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317201.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O amino ácido lisina é catabolizado em plantas e animais pela via da sacaropina. Nesta via, a lisina é convertida a ?-aminoadipato-?-semialdeído (AASA) pela ação da enzima bifuncional lisina-cetoglutarato redutase/sacaropina desidrogenase (LKR/SDH). O intermediário AASA é então convertido a ?-aminoadipato (AAA) pela enzima ?-aminoadipato-?-semialdeído desidrogenase (AASADH). A LKR/SDH já foi bem caracterizada em plantas e animais, mas a atividade enzimática bem como o possível papel fiiológico da AASADH ainda não foi demonstrada em plantas. A via da sacaropina, além do seu importante papel na regulação dos níveis de lisina, está também envolvida em processos de resposta a estresses. Este trabalho está dividido em dois capítulos. No capítulo I descrevemos a identificação do gene que codifica a AASADH em milho e a caracterização da atividade enzimática da enzima em endosperma imaturo de milho. Mostramos que a AASADH é codificada pelo gene Aldh7b1, um gene muito conservado em eucariotos. A enzima codificada pelo gene Aldh7b1 foi parcialmente purificada de endosperma imaturo de milho e através de eletroforese em condições denaturantes e cromatografia em coluna de gel filtração mostramos que a enzima, na sua forma nativa, apresenta-se como um tetrâmero constituído por quatro subunidades de 55 kDa. A AASADH isolada de endosperma imaturo de milho converte o semi-aldeido AASA em AAA. O produto da reação catalisada pela AASADH foi confirmado por cromatografia em camada delgada. No capítulo II discutimos o papel da via sacaropina no desenvolvimento da semente e na resposta de planas jovens de milho a estresses abióticos. As enzimas LKR/SDH e AASADH são co-expressos nas células das camadas da sub-aleurona do endosperma de milho nas fases intermediarias do desenvolvimento. No entanto, embora a proteína AASADH seja produzida no endosperma e no embrião de sementes imaturas e nos tecidos de plantas jovens, a proteína LKR/SDH é detectada unicamente nas células da sub-aleurona das sementes imaturas. A AASADH mostrou atividade máxima a pH 7,4 e Kms para AASA e NAD+ na ordem de micromolar. Em endosperma imaturo a via da sacaropina é induzida por lisina e reprimida por estresse salino, enquanto prolina e ácido pipecólico são significativamente reprimidos por lisina. Em coleóptiles jovens as enzimas LKR/SDH e AASADH são induzidas transcricionalmente por estresses salino, osmótico e oxidativo, mas enquanto que a proteína AASADH acumula nos tecidos sob estresse, a proteína LKR/SDH não é detectada. Nossos resultados indicam que os genes que codificam as enzimas LKR/SDH e AASADH são co-expressos a nível transcricional, mas não a nível traducional. A ausência da proteína LKR/SDH em plantas jovens sob estresses e os altos níveis do seu transcrito serem detectados mostra um desacoplamento transcrição/tradução que podem ter consequências regulatórias ainda desconhecidas
Abstract: Lysine is catabolized in developing plant tissues through the saccharopine pathway. In this pathway, lysine is converted into ?-aminoadipate-?-semialdehyde (AASA) by the bifunctional enzyme lysine-ketoglutarate reductase/saccharopine dehydrogenase (LKR/SDH). AASA is then converted into ?-aminoadipate (AAA) by aminoadipic semialdehyde dehydrogenase (AASADH). LKR/SDH was characterized in higher eukaryotes, but AASADH has not been demonstrated in plants. Furthermore, studies have shown that besides the degradation of lysine, the saccharopine pathway is involved in stress response processes in plants, animals and bacteria. This work was divided into two chapters. Chapter I describes the identification of the gene encoding AASADH and the partial purification and characterization of the enzyme from developing maize endosperm. The enzyme AASADH is encoded by the Aldh7b1 gene, a gene highly conserved among eukaryotes. The enzyme partially purified from developing endosperm and analyzed by SDS-PAGE and gel filtration chromatography behaved, in its native form, as a tetramer constituted by four monomers of 55 kDa. The enzymatic convertion of AASA into AAA was verified by thin layer chromatography. In Chapter II the role of the saccharopine pathway in seed development and stress responses is discussed. LKR/SDH and AASADH are co-expressed in the sub-aleurone cell layers of the developing endosperm; however, although AASADH protein is produced in reproductive and vegetative tissues, the LKR/SDH protein is detectable only in the developing seeds. AASADH showed an optimum pH of 7.4 and Kms for AASA and NAD+ in the micromolar range. In the developing endosperm the saccharopine pathway is induced by exogenous lysine and repressed by salt stress, whereas proline and pipecolic acid synthesis are significantly repressed by lysine. In young coleoptiles the LKR/SDH and AASADH transcriptions are induced by abiotic stress, but while the AASADH protein accumulates in stressed tissues, LKR/SDH does not. Our results indicate that the genes encoding the LKR/SDH and AASADH enzymes are co-expressed at the transcriptional level, but not the translational level. The absence of LKR/SDH protein in young plants under stress despite of the high levels of transcripts being detected suggests a decoupling transcription/translation that may have regulatory consequences yet unknown
Doutorado
Genetica Vegetal e Melhoramento
Doutor em Genetica e Biologia Molecular
Thelander, Mattias. "Studies of molecular mechanisms integrating carbon metabolism and growth in plants /." Uppsala : Dept. of Plant Biology and Forest Genetics, Swedish Univ. of Agricultural Sciences, 2003. http://epsilon.slu.se/a432.pdf.
Full textGoodsall, Christopher William. "The role of pyrophosphate dependent phosphofructokinase in Crassulacean acid metabolism plants." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361686.
Full textHäkkinen, Suvi T. "A functional genomics approach to the study of alkaloid biosynthesis and metabolism in Nicotiana tabacum and Hyoscyamus muticus cell cultures /." [Espoo, Finland] : VTT, 2008. http://www.vtt.fi/inf/pdf/publications/2008/P696.pdf.
Full textClermont, Kristen Renee. "Plasticity of Primary Metabolism in Parasitic Orobanchaceae." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/86130.
Full textPh. D.
Parasitic weeds of the plant family Orobanchaceae attach to the roots of host plants via haustoria. Parasite haustoria embed into the host plant and are capable of drawing nutrients from host vascular tissue. Species in this family span the spectrum of the extent to which a parasitic plant may depend on its host for nutrients. This allows comparisons that provide insight into the ways in which parasites adapt. A key aspect of this is the relationship between the metabolite profile of the parasite and the metabolite profile of the host. To what extent does the metabolite profile of the parasite depend on that of the host? Do parasites that differ in host-dependency also differ in their metabolism or do they use common metabolic strategies? These questions were addressed using comparative profiling of primary metabolites to gain insight into carbon and nitrogen assimilation by the obligate parasite Phelipanche aegyptiaca (which cannot perform photosynthesis) and the facultative parasite Triphysaria versicolor (which can perform photosynthesis). First, metabolite profiles of these parasites and their hosts were compared during the key life stages before and after haustorial attachment. Second, the impact of specific variations in host metabolism was analyzed for P. aegyptiaca growing on Arabidopsis thaliana hosts. These hosts had mutations in enzymes related to amino acid metabolism but otherwise identical genetic backgrounds. Comparison of P. aegyptiaca and T. versicolor metabolite profiles identified substantial differences in the stages spanning the transition from pre-haustorial development through post-haustorial feeding. Each parasite species is distinct from the other and from their hosts. For parasites growing on host lines that differ in amino acid content, the size of P. aegyptiaca tubercles decreased when grown on the aap6 mutant line, which has decreased levels of asparagine in the phloem sap compared to the wild type. However, altered amino acid levels in other lines did not impact P. aegyptiaca growth, indicating that this parasite has ability to compensate for variation in host metabolic composition. Overall, this work provides insights both into the metabolism of parasitic plants and lays the foundation for the development of new metabolism-based control strategies.
Fank-de-Carvalho, Suzane Margaret. "Contribuição ao conhecimento da anatomia, micromorfologia e ultraestrutura foliar de Amaranthaceae do Cerrado." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317998.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: A família Amaranthaceae no sensu lato, incluindo Chenopodiaceae, é formada por cerca de 2.360 espécies, 145 delas encontradas no Brasil; 94 espécies subsistem em diversas fitofisionomias do Bioma Cerrado, 71 são endêmicas de diferentes regiões e biomas brasileiros e 27 aparecem em listas regionais de espécies ameaçadas de extinção. Visando contribuir para o conhecimento das espécies dessa família, foram estudados aspectos ecológicos, micromorfológicos, anatômicos e ultraestruturais de folhas de Amaranthaceae nativas do Brasil, com ênfase nos gêneros Alternanthera, Gomphrena, Froelichiella, Hebanthe e Pfaffia. As espécies nativas de regiões abertas de cerrado apresentam adaptações que favorecem a sobrevivência em condições adversas (seca e fogo), tais como raízes tuberosas ou lenhosas e xilopódios, hábito herbáceo e/ou subarbustivo, pilosidade densa nas porções aéreas, senescência de ramos aéreos durante as fases mais secas, dependência de fogo ou chuva para rebrotação e/ou floração, frutificação rápida seguida de dispersão anemocórica, epidermes com cutículas bem desenvolvidas e metabolismo fotossintético C4. O comportamento pirofítico das espécies favorece o estabelecimento pioneiro das mesmas, principalmente nas áreas abertas do Cerrado. As superfícies foliares de algumas espécies do gênero Gomphrena apresentam cristais de cera epicuticular do tipo plaquetas, orientadas paralelamente, aspecto anteriormente descrito apenas em espécies de Chenopodiaceae. Em duas espécies foram encontrados fungos Ascomyceto colonizando folhas, cujos aspectos ultraestruturais foram descritos. A anatomia Kranz foi caracterizada em seis espécies do gênero Gomphrena, que também possuem cloroplastos dimórficos, demonstrando estrutura foliar compatível com o metabolismo fotossintético C4. Na análise das duas espécies do gênero Alternanthera (uma C3 e outra intermediária C3-C4) verificou-se que a posição das organelas nas células da bainha pode ser um elemento chave na determinação do tipo metabólico. As espécies de Froelichiella, Hebanthe e Pfaffia possuem anatomia e ultraestrutura compatíveis com o metabolismo C3. A anatomia foliar e a ultraestrutura das espécies estudadas apresentam um padrão já descrito para outras espécies da família Amaranthaceae, exceto para os gêneros Hebanthe e Froelichiella, cuja descrição anatômica e ultraestrutural foi realizada pela primeira vez. A evolução do metabolismo C4 pode estar relacionada, pelo menos em parte, ao desenvolvimento da anfiestomia associada à maior espessura do limbo foliar em espécies herbáceas. Plastoglóbulos bem desenvolvido foram encontrados em cloroplastos de algumas das espécies do Cerrado e parecem associados aos mecanismos de defesa, além do metabolismo de lipídios. A família Amaranthaceae pode ser um bom marcador da biodiversidade de dicotiledôneas de pequeno porte e da capacidade de regeneração das áreas de campos rupestres, campos úmidos e outros tipos de vegetação aberta dos cerrados. A riqueza de informações obtidas durante o estudo de espécies dessa família ilustram a importância da ampliação das pesquisas básicas e aplicadas em suas espécies, especialmente as que ocorrem naturalmente no Cerrado. A ampliação do conhecimento relativo à anatomia e à especialização das organelas de diferentes gêneros para o metabolismo fotossintético pode contribuir para o entendimento da evolução da via C4 e do ambiente onde as plantas se especializaram. O estudo do Bioma Cerrado pretende ampliar as justificativas para esforços de preservação de sua biodiversidade.
Abstract: The Amaranthaceae family sensu lato, including Chenopodiaceae, is comprised of approximately 2,360 species, 145 of them found in Brazil. 94 species exist in various vegetation types of the Cerrado, 71 are endemic to different regions and Brazilian biomes, and 27 appear in regional lists of endangered species. To contribute to the knowledge of this family species the ecological aspects, micromorphology, anatomy and ultrastructure of leaves of brazilian Amaranthaceae were studied, with emphasis on the genera Alternanthera, Gomphrena, Froelichiella, Hebanthe and Pfaffia. The native species of open areas of Cerrado (a savannah-like vegetation) exhibit adaptations that promote survival in adverse conditions (drought and fire), such as tuberous or woody roots, xylopodium, herbaceous or subshrub habit, dense pubescence in aerial portions, senescence of shoots and leaves during the driest season, dependence on rain or fire to resprout and/or flowering, fruiting followed by rapid wind dispersion, tick cuticle on epidermis and C4 photosynthetic metabolism. The species' fire behavior favors their establishment as pioneers, especially in open areas of the Cerrado. Leaf surfaces of some species of the genus Gomphrena present epicuticular wax crystals in platelet form, oriented in parallel, an aspect previously described only in Chenopodiaceae species. In two of the species studied Ascomycete fungi were found colonizing its leaves, and ultrastructural aspects were described. Kranz anatomy was found in six species of the genus Gomphrena, which also have dimorphic chloroplasts, showing leaf structure compatible with the C4 photosynthetic metabolism. In the analysis of two species of Alternanthera (a C3 and a C3-C4 intermediate) showed that besides the Kranz anatomy, the position of organelles in bundle sheath cells can be a key element in determining the metabolic type. The Froelichiella, Hebanthe and Pfaffia species have leaf anatomy and ultrastructure consistent with C3 metabolism. The leaf anatomy and ultrastructure have a pattern already described for other species of the Amaranthaceae family, except for the genera Hebanthe and Froelichiella, whose anatomical and ultrastructural aspects were described for the first time. The evolution of C4 metabolism may be related, at least in part, to the development of amphystomy associated with increased leaf thickness in herbaceous species. Large plastoglobuli were found in chloroplasts of some Cerrado species and appear associated with defense mechanisms, and lipid metabolism. The Amaranthaceae family can be a good marker of the biodiversity of nonwoody Eudicotyledons with ability to regenerate areas of rocky fields, wet grasslands and other open vegetation of the cerrado. These results illustrate the importance of expansion of basic and applied research in Amaranthaceae species, especially those that occur naturally in Brazilian cerrados. The expansion of knowledge concerning the anatomy and the specialization of organelles of different genera to perform its photosynthetic metabolism may contribute to understanding of the evolution of C4 pathway and the environment where plants specialize. The study of Cerrado Biome aims to broaden the justification for efforts to preserve its biodiversity.
Doutorado
Biologia Celular
Doutor em Biologia Celular e Estrutural
Shank, Karin Janel. "Coordination of the endoplasmic reticulum stress response and lipid metabolism in plants." NCSU, 2000. http://www.lib.ncsu.edu/theses/available/etd-20000729-161702.
Full textThe endoplasmic reticulum (ER) stress response is an important signal transduction pathway that senses ER stress caused by misfolded proteins or increased secretory protein traffic and induces molecular chaperone expression to counter such stress. The response has been well characterized in yeast and mammals where it has been associated with a variety of metabolic pathways, such as phospholipid biosynthesis, translational inhibition, and ER associated degradation. In plants, however, the connections of the ER stress response with metabolic pathways other than those involved in chaperone biosynthesis have not been characterized. This study defines a connection between phospholipid synthesis and the ER stress response in plants. Two model systems were used to characterize this association, the maize mutant floury-2 (fl2), which displays a unique endosperm specific ER stress response mediated by a mutant seed storage protein, and soybean cell cultures treated with the pharmacological agents tunicamycin (Tm) or azetidine-2-carboxylic acid (AZC). These chemicals interfere with normal protein synthesis and processing events, and are well characterized inducers of ER stress responses in animals, plants, and yeast. Investigation of both of these systems revealed a common theme; induction of the ER stress response in plants leads to increased activity and/or expression of various phospholipid biosynthetic enzymes. These increases were correlated with previously described amplifications in expression of the molecular chaperones binding protein (BiP), protein disulfide isomerase (PDI) and calreticulin. Certain aspects of the ER stress response may be unique to plants. A seed-specific result of the ER stress response was the accumulation of triacylglycerols (TG), which specifically increased in the endosperm of the fl2 mutant to more than 3-fold higher than normal endosperm levels by 36 days after pollination (DAP). The maize and soybean systems used in this study provide a starting point for the investigation of other details of the ER stress response in plants and represent important tools for future efforts to define the components of the signaling pathway.
Barco, Brenden Lee. "Evolution, Regulation, and Function of Tryptophan-Derived Secondary Metabolism in Mustard Plants." Thesis, Yale University, 2019. http://pqdtopen.proquest.com/#viewpdf?dispub=13851840.
Full textPlants produce a variety of small molecules, including those essential for survival in all conditions (primary metabolites) or for more ecologically specific conditions (secondary metabolites). While primary metabolic pathways are broadly shared among plants, secondary metabolism is under constant selective pressure towards chemical innovation, given the continual fluctuation of the environment. Thus, plant secondary metabolism - whose constituents number in the hundreds of thousands - is lineage-specific, highly structurally diverse, and ultimately of high value to medicine, agriculture, and industry. Efforts to optimize the production of specific metabolites or to discover new compounds remain difficult primarily due to inadequate understandings of the metabolic genes involved and how these genes are regulated. This work first examines co-regulation, a major form of organization by which plant secondary metabolic genes are organized. In response to the bacterial crop pathogen Pseudomonas syringae, Arabidopsis thaliana and its relatives in the mustard family produce numerous secondary metabolites from the amino acid tryptophan, including the antimicrobial compound camalexin. However, hundreds of biosynthetic genes of unknown function are also simultaneously upregulated. Using metabolic profiling and co-expression analysis, I helped to identify the complete biosynthetic pathway to the indole-3-carbonylnitriles (ICNs), a previously unknown class of compounds. When the cytochrome P450 gene CYP82C2 is mutated, biosynthesis of the compound 4-hydroxy-ICN (4OH-ICN) is abolished, and plant defense against P. syringae is impaired. Conversely, addition of 4OH-ICN to plants is sufficient to suppress bacterial growth. Next, this work examines the evolution of camalexin and 4OH-ICN metabolism. Cytochrome P450-directed secondary metabolism has been shown almost without exception to be evolutionarily derived from changes to enzymes with broad substrate specificity. By contrast, I observe through genetics, enzyme phylogenetic analysis, and transient expression assays that the ICN and camalexin biosynthetic pathways evolved from a common chemical substrate. In particular, changes to camalexin catalysis by the newly duplicated gene CYP71A12 led to the formation of ICN metabolism in several mustard species, although both compounds are directly derived from indole cyanohydrin. Furthermore, 40H-ICN is an extremely recently evolved metabolite, derived from a flurry of genic, epigenetic and transposon-mediated rearrangements of a yet-more recent gene duplicate (CYP82C2). These regulatory changes to CYP82C2 lead to its pathogen-inducibility solely in the species A. thaliana. I additionally identify WRKY33 and MYB51 as two sets of defense regulators that carefully fine-tune 40H-ICN metabolism by direct biosynthetic gene regulation. WRKY33 transcription factor, which is involved in the species-specific regulation of CYP82C2, is conserved throughout flowering plants, indicating that transcriptional recruitment is an important feature in the expansion of secondary metabolism. Finally, this work probes possible molecular functions of 40H-ICN and camalexin by exploring the molecular mechanisms underlying their secretion from roots and regulation of cell death processes. This study ultimately reveals that the proliferation of diverse chemical arsenals in plants is greatly aided by the regulatory capture of new and rapidly evolving genes by evolutionarily more stable transcription factors. Future emphases on transcriptional regulators of secondary metabolism may thus aid in the discovery of new secondary metabolic pathways on a more rapid scale.
Dutta, Indrani. "Boron Deficiency and its Associated Perturbation of Nucleotide Metabolism in Tomato Plants." Thesis, North Texas State University, 1987. https://digital.library.unt.edu/ark:/67531/metadc798226/.
Full textConselvan, Giovanni Battista. "Biostimulant effects of different products on the metabolism of agro-food plants." Doctoral thesis, Università degli studi di Padova, 2018. http://hdl.handle.net/11577/3424986.
Full textL'agricoltura conservativa rappresenta la strategia principale per soddisfare le crescenti esigenze alimentari e contribuire a un pianeta stabile. Per raggiungere questi obiettivi, una delle soluzioni più promettenti è l'adozione dei biostimolanti. I biostimolanti sono composti naturali che, a basse concentrazioni, sono in grado di migliorare l'assorbimento dei nutrienti delle piante, l'efficienza dell’attività dei nutrienti, la tolleranza agli stress abiotici / biotici e la resa delle colture. Le sostanze umiche (HS) sono considerate una classe di biostimolanti, in quanto hanno effetti positivi sull'assorbimento di macro e micronutrienti, migliorano considerevolmente il metabolismo, la crescita e le rese delle colture agricole. Con questo background, gli obiettivi di questo progetto di dottorato sono stati di i) valutare lo stato metabolico e biochimico delle piante trattate con HS per chiarire i meccanismi di azione dei biostimolanti. Ciò potrebbe consentire lo sviluppo di una seconda generazione di biostimolanti in cui meccanismi di azione sinergici e complementari possono essere considerati. ii) studiare potenziali effetti biostimolanti di un composto fungicida. Nel primo articolo le piante di Arabidopsis thaliana sono state trattate con HS per otto ore. Le proteine delle radici sono state studiate mediante spettrometria MS accoppiata con la tecnica iTRAQ (Isobaric Tag for Relative and Absolute Quantification). Per le radici trattate con HS sono stati identificate 902 proteine, dove 291 proteine erano espresse in modo differenziale. Strumenti bioinformatici come DAVID, KEGG, IIS e Cytoscape sono stati utilizzati per interpretare le funzioni biologiche, l'analisi e la visualizzazione delle proteine connesse identificate. Da questa analisi, è stato possibile valutare che la maggior parte delle proteine differenzialmente espresse sono state funzionalmente classificate in risposta alle sostanze inorganiche, redox omeostasi, metabolismo energetico, sintesi proteica, traffico e divisione cellulare. Con il presente studio è stata fornita una panoramica sulle vie metaboliche più influenzate dall'attività biologica delle HS. Inoltre, dall'analisi degli interattomi e dei cluster di DAVID è stato possibile osservare nuovi effetti di HS, come per esempio sugli interattomi dell’Ubiquitina e del RACK1A. Nel secondo articolo, le piantine di Arabidopsis thaliana, coltivate in condizioni idroponiche, sono state trattate per 8 ore con acido indol-3-acetico (IAA), HS da International Humic Substances Society (IHSS) e HS da feci di lombrico (EF), rispettivamente. Le caratteristiche strutturali delle sostanze umiche sono state analizzate mediante spettroscopie 1H NMR e FT-IR. Nelle foglie e nelle radici, mediante gascromatografia-spettrometria di massa (GC / MS) e tecniche di cromatografia liquida-spettrometria di massa (LC / MS) sono stati identificati e quantificati gli amminoacidi, zuccheri e il contenuto di carboidrati liberi, e gli amminoacidi fogliari ottenuti dall'idrolisi proteica. L'analisi discriminante canonica (CDA) è stata utilizzata per valutare l'influenza dei trattamenti sui parametri studiati. Il trattamento con EF ha avuto la massima influenza sui profili dei metaboliti rispetto al controllo, IAA e IHSS. L'analisi del CDA ha evidenziato una chiara distinzione tra le risposte fisiologiche delle piante trattate con EF e IHSS, in base alle diverse proprietà chimiche e strutturali delle HS. Le piante trattate con IAA non sono invece risultate significativamente differenti dal controllo. Una migliore comprensione degli effetti specifici delle diverse HS, anche in relazione alle loro caratteristiche chimiche, potrebbe servire come base per l'identificazione di composti marcatori per la bioattività delle HS. Nel terzo articolo, l'attività biostimolante delle HS estratte da quattro leonarditi è stata analizzata su piantine di mais. Dopo 48 ore di trattamento con 5 concentrazioni (0, 0,1, 0,5, 1 e 10 mg CL-1) di HS, sono stati analizzati la crescita e la morfologia delle radici, l’attività della glutammina sintetasi (GS), l’attività del glutammato sintasi (GOGAT), il contenuto proteico totale, il contenuto di zuccheri solubili, l'attività della fenilalanina ammonia-liasi (PAL), i fenoli solubili e gli acidi fenolici liberi. Le HS estratte da diverse leonarditi avevano un pattern spettroscopico simile, ma con piccole differenze. HS_USA ha indotto una maggior crescita delle radici, della superficie radicale e della proliferazione delle radici secondarie. HS_4, HS_USA e HS_B hanno determinato una miglior efficienza dell'uso dei nutrienti delle piante, incrementando l'attività degli enzimi GS e GOGAT e la produzione totale di proteine. Le quattro HS hanno stimolato anche l'attività dell'enzima PAL, seguita da una maggiore produzione di fenoli solubili totali. Questo studio ha rilevato che, sebbene l'attività delle HS dipendesse dall'origine della leonardite, questi composti possono essere attribuiti ai prodotti biostimolanti, poiché stimolano la crescita delle piante, il metabolismo dell'azoto e l'accumulo di sostanze fenoliche. Nel quarto articolo è stato studiata l’azione biostimolante del sedaxane, intesa come attività secondaria. Sono stati analizzati i cambiamenti fisiologici in piantine di mais all'aumentare delle dosi di applicazione (25, 75 e 150 μg a.i. seme-1) in condizioni sterili controllate (piante non infette). Sedaxane ha dimostrato avere effetti di tipo auxino- e gibberellino-simili significativi, con i massimi benefici raggiunti alla dose intermedia. La lunghezza della radice (+ 60% rispetto ai controlli non trattati), l'area (+ 45%) e le biforcazioni (+ 51%) sono stati significativamente influenzati. Sedaxane ha incrementato l'attività della glutammina sintetasi (GS) delle foglie e delle radici con conseguente maggiore accumulo di proteine. Sedaxane ha anche incrementato l'attività della fenilalanina ammonia-liasi (PAL) nelle foglie, che potrebbe essere responsabile dell'aumento dell'attività antiossidante (acidi fenolici). Si è concluso che, oltre al suo effetto protettivo, il sedaxane può facilitare lo sviluppo delle radici e intensificare il metabolismo dell'azoto e dei fenilpropanoidi nelle giovani piante di mais e può essere utile per superare gli stress biotici e abiotici nelle prime fasi di crescita. Come conclusione generale, anche se le HS sono state estratte da diverse matrici e applicate su diverse specie di piante, queste hanno mostrato attività biologiche simili. Studi di proteomica e metabolomica hanno confermato che le tecniche "-omics" sono strumenti essenziali per avere una visione "panoramica" sui cambiamenti metabolici che avvengono all'interno di un organismo dopo una perturbazione esterna positiva o negativa.
Waters, Ormonde Dominick Creagh. "Metabolism and infection in the Stagonospora nodorum-wheat pathosystem /." Murdoch University Digital Theses Program, 2008. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20090409.123159.
Full textColling, Janine. "Towards understanding the metabolism of in vitro Sutherlandia frutescens (L.)R.Br. cultures." Thesis, Stellenbosch : University Stellenbosch, 2009. http://hdl.handle.net/10019.1/4601.
Full textENGLISH ABSTRACT: Sutherlandia frutescens (L.) R. Br., also regarded as Lessertia frutescens, is a leguminous, perennial shrub indigenous to South Africa. Extracts prepared from the leaves have traditionally been used for the treatment of various diseases. Reports have also indicated that S. frutescens provides certain health benefits to cancer and HIV/AIDS patients. Analysis of extracts indicated the presence of several compounds (bitter triterpenoid glycosides, several flavonoids, amino acids, small amounts of saponins (no alkaloids though), asparagine, Larginine, canavanine, gamma-aminobutyric acid (GABA) and pinitol) which contribute to the medicinal properties of this plant. The first part of this study involved testing the effect of six treatments (light, dark, soaking of seeds, physical scarification, chemical scarification and flaming of seeds) on the in vitro germination of Sutherlandia seeds to elucidate the factors which control seed germination. Those treatments which removed the seed coat were most successful for germination with physical scarification being the most efficient method, resulting in 98.6% of the seeds germinating after 21 days. Although the organogenesis of Sutherlandia explants (cotyledons and hypocotyls) in vitro were investigated (results not included in this thesis), omitting plant growth regulators (PGR) in the cultivation medium was best for shoot multiplication. However, this PGR-free system successfully provided a continuous supply of plant material for further studies. It would be possible to successfully adopt it for commercial production of plants to assist with cultivation of Sutherlandia as a field crop. Another advantage of this system is spontaneous rooting with 85% of the in vitro microshoots rooting in PGR-free medium. These rooted plants were acclimated in the glasshouse using vented lids to harden off the shoots and this method resulted in 100% survival of plants. The second part of this study investigated the induction of hairy root cultures of S. frutescens using Agrobacterium-mediated transformation. The efficiency of three Agrobacterium strains (A4T, LBA9402 and C58C1) to transform different S. frutescens explants (cotyledons and hypocotyls) was analyzed. All three strains were equally efficient at inducing hairy roots in both hypocotyls and cotyledons. However, transformation of S. frutescens was dependent on the type of explant used with the hypocotyls being more efficiently transformed than the cotyledons. Overall the transformation of both the hypocotyl (93%) and cotyledon (47%) was highest when the strain A4T was used. Four hairy root clones were selected and their cultivation in a liquid system was optimized by investigating their growth in four different types of media (Gamborg B5 (Gamborg et al., 1968), White’s (White, 1934; White, 1954), MS (Murashige and Skoog, 1962) and half strength MS medium). All the growth of hairy root clones was best in the B5 and MS medium, with White’s medium being the least effective cultivation medium. Molecular analysis of hairy roots was used to prove the transgenic status of these four putative transgenic clones. This was achieved using polymerase chain reaction (PCR) amplification of rol A (320 bp), B (780 bp) and C (600 bp) genes to determine the presence of the TL-DNA in the plant genome. During Southern hybridization a radioactively labeled rol A probe was used to determine the copy number of the rol A gene. The three rol genes were present in all four hairy root clones. The third part of this study focused on the effect of three abiotic stress factors (nitrogen availability, salinity and drought) on the synthesis of four metabolites (gamma-aminobutyric acid (GABA), asparagine, arginine and canavanine). The effect of nitrogen availability on metabolite synthesis and the morphology was determined using in vitro shoot cultures as well as the hairy root clone C58C1-g. Nitrogen availability studies were conducted by cultivating the microshoots or root tips on modified MS medium. The MS medium contained either the normal amount of nitrogen (1.9 g L-1 KNO3 and 1.65 g L-1 NH4NO3) in the MS medium (1x nitrogen), half the normal nitrogen concentration in MS medium (0.5x nitrogen) or twice the normal nitrogen concentration in MS medium (2x nitrogen). The arginine and asparagine levels in the roots and shoots and the canavanine level in the shoots were directly correlated with the amount of nitrogen in the medium (as the nitrogen level increased, the metabolite levels increased). The GABA level in the shoots was inversely correlated with the amount of nitrogen in the medium. Several reasons may explain these metabolic changes including the assimilation of extra nitrogen into asparagine, canavanine and arginine in the shoots. The reduced GABA levels may indicate the preferential flux of the free GABA into other nitrogen assimilatory pathways such as protein synthesis as well as its rapid utilization to replenish the tricarboxilic acid cycle intermediates. The effect of water (induced by including 3% (w/v) PEG in the medium) and salt stress (induced by including either 50 or 100 mM NaCl in the medium) was only investigated in the shoot cultures as the root cultures lacked the synthesis of canavanine. Water stress did not significantly alter the metabolite levels, but resulted in a significant decrease in the growth (fresh weight and total shoot length) and the rooting response of these microshoots. Salt stress only resulted in a significant increase in arginine levels with increasing salinity and also caused a reduction in the rooting and growth response. Lowered plant vigour may be the first visual sign of water stress. Addition of NaCl may lead to ion toxicity and requires osmotic adjustment resulting in changes at the metabolic level concomitant to physiological growth changes. Finally, the anti-bacterial activity and the phytochemistry of transgenic root cultures and untransformed in vitro and ex vitro plant material was examined. Only the extracts prepared from the wild harvested leaf material exhibited moderate anti-bacterial activity (1.25 mg ml-1) against all the bacteria (Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis and Staphylococcus aureus) tested. Changes to the secondary metabolism of hairy roots were investigated using TLC and LC-MS analysis. Several of the compounds in the hairy root extracts were present in higher levels than in the control root extracts. Transformation also increased the complexity of the phytochemical pattern of the hairy roots, either due the synthesis of novel compounds or upregulated synthesis of existing metabolic pathways. The production of hairy roots and the establishment in a liquid system during this study was an important step towards upscaling these cultures to a bioreactor. In future these roots can assist in developing cultures which produce a high yield of the desired metabolites.
AFRIKAANSE OPSOMMING: Sutherlandia frutescens (L.) R. Br., ook bekend as Lessertia frutescens is ‘n peulagtige meerjarige struik, inheems tot Suid Afrika. Ekstrakte wat van die blare voorberei word, is tradisioneel gebruik vir die behandeling van verskeie siektes. Berigte het ook daarop gedui, dat S. frutescens sekere gesondheidsvoordele vir kanker en HIV/VIGS pasiënte inhou. ‘n Ontleding van die ekstrakte, dui op die teenwoordigheid van verskeie verbindings (bitter triterpenoïed glikosiede, verskeie flavonoïede, aminosure, klein hoeveelhede saponiene (alhoewel geen alkaloïede), asparagien, L-arginien, canavanien, gamma-aminobottersuur (GABS) en pinitol) wat tot die medisinale eienskappe van hierdie plant bydrae. Die eerste deel van die studie het die effek van ses behandelings (lig, donker, week van sade, fisiese skarifikasie, chemiese skarifikasie en die vlam van sade) op die in vitro ontkieming van Sutherlandia sade getoets met die doel om die faktore wat saadontkieming beheer, te identifiseer. Die beste behandeling vir saadontkieming was dié behandelings wat die saadhuid verwyder het. Die mees effektiewe metode van saadhuidverwydering was die fisiese skarifikasie van sade, wat gelei het tot ‘n 98.6% ontkieming van sade na 21 dae. Alhoewel in vitro organogenese van Sutherlandia eksplante (kotiel en hipokotiel) ondersoek was (resultate nie ingesluit in die tesis nie), was plant groei reguleerders (PGR) uitgesluit in die groeimedium om stingelvermeerdering te bevorder. Nie te min was die PGR-vrye sisteem suksesvol om ‘n voortdurende bron van plant material vir verder studies te verskaf. Dit sou egter moontlik wees om die PGR-vrye sisteem suksesvol te kon aanpas vir die kommersiële produksie van plante met die doel om Sutherlandia as ‘n landbougewas te bevorder. ‘n Verdere voordeel van dié sisteem, is die spontane wortelvorming, met 85% van die in vitro mikrostingels wat wortels in die PGR-vrye medium produseer het. Hierdie bewortelde plante was in die glashuis geakklimatiseer met behulp van geventileerde deksels (vir stingel afharding) en het tot ‘n 100% oorlewing gelei. Die tweede deel van die studie het die induksie van S. frutescens harige wortelkulture met behulp van Agrobacterium-bemiddelde transformasie ondersoek. Die effektiwiteit van drie Agrobacterium stamme (A4T, C58C1 en LBA9402) om verskillende S. frutescens eksplante (kotiel en hipokotiele) te transformeer, was geanaliseer. Al drie stamme was ewe effektief om harige wortels op beide hipokotiel en kotiele te induseer. S. frutescens transformasie blyk egter tog van die tipe eksplant afhanklik te wees, aangesien die hipokotiele meer effektief as die kotiele getransformeer kon word. Met inagneming van beide die hipokotiel (93%) en kotiel vii (47%), was transformasie optimaal met die gebruik van die A4T stam. Vier harige wortelklone was geselekteer en hulle produksie in ‘n vloeibare sisteem was geoptimiseer deur hulle groei in vier verskillende tipe media (Gamborg B5 (Gamborg et al., 1968), White’s (White, 1934; White, 1954), MS (Murashige and Skoog, 1962) en half-sterkte MS medium) te ondersoek. B5 en MS medium was beskou as die beste vir alle die harige wortelklone se groei, terwyl White’s medium die minste doeltreffende groeimedium was. Molekulêre analise van die harige wortels was gebruik ten einde die transgeniese status van die vier vermoedelike transgeniese klone te bewys. Dit was behaal deur polimerase kettingreaksie amplifisering (PKR) van die rol A, B en C gene ten einde die teenwoordigheid van die TL-DNS in die plant genoom aan te toon. Tydens Southern hibridisasie was ‘n radioaktief gemerkte peiler gebruik om die aantal rol A geen kopieë te bepaal. Die drie rol gene was teenwoordig in al vier harige wortelklone. Die derde deel van die studie het gefokus op die effek van drie abiotiese stress faktore (stikstof beskikbaarheid, sout- en droogte stres) op die produksie van vier metaboliete (GABS, asparagien, canavanien en arginien). Die effek van stikstof beskikbaarheid op die metaboliet produksie asook die morfologie was bestudeer deur gebruik te maak van in vitro mikrostingels asook die harige wortel kloon C58C1-g. Stikstof beskikbaarheidstudies was uitgevoer deur die mikrostingels of wortelpunte in ‘n gewysigde MS medium te groei. Die MS medium was aangepas om die normale hoeveelheid stikstof (1.9 g L-1 KNO3 en 1.65 g L-1 NH4NO3) in MS medium (1x stikstof), of die helfte van die normale stikstof konsentrasie (0.5x stikstof) of twee keer die normale stikstof konsentrasie in MS medium (2x stikstof) te bevat. Die arginien en asparagien vlakke in die wortels en stingels, asook die canavanien vlak in die stingels was positief gekorreleerd aan die stikstof konsentrasie in die medium. Die GABS vlak in die stingels was egter omgekeerd eweredig aan die stikstof konsentrasie in die medium. Verskeie redes kan aangevoer word om die metaboliet veranderinge te verduidelik, insluitende die assimilasie van addisionele stikstof in asparagien, canavanien en arginien in die stingels. Die verlaagde GABS vlakke kan dui op die voorkeur van vrye GABS vloei na ander stikstofassimilerende metaboliese paaie soos proteïen sintese, asook die snelle benutting van GABS ten einde die Trikarboksielsuursiklus intermediêre produkte aan te vul. Die effek van droogte (geïnduseer deur die byvoeging van 3% (m/v) PEG tot die medium) en sout stres (geïnduseer deur 50 of 100 mM NaCl byvoeging tot die medium) was slegs in die stingel kulture ondersoek weens die afwesigheid van canavanien produksie in die wortel kulture. Water stres het nie ‘n betekenisvolle verandering in die metaboliet vlakke meegebring nie, maar dit het wel tot ‘n beduidende afname in groei (vars massa en totale stingel lengte) en bewortelingsreaksie in die mikrostingels gelei. Sout stres het slegs tot ‘n betekenisvolle viii toename in arginien vlakke asook ‘n afname in die wortelvorming en groeireaksie tydens die toenemende sout vlakke gelei. ‘n Verlaging in plant groeikragtigheid mag ‘n eerste visuele teken van water stres wees. Die toevoeging van NaCl tot die medium kan tot ioontoksisiteit lei en plante reageer deur middel van osmotiese aanpassing wat tot veranderinge in die metaboliet vlakke asook veranderinge in fisiologiese groei, lei. Die finale deel van die studie het die anti-bakteriële aktiwiteit en die fitochemie van die transgeniese wortel kulture asook die ongetransformeerde in vitro en ex vitro plant materiaal ondersoek. Slegs die ekstrakte verkry vanaf blaar materiaal geoes uit die natuur, het matige anti-bakteriële aktiwiteit (1.25 mg ml-1) teen al die bakterië (Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis en Staphylococcus aureus) wat ondersoek is, getoon. Aanpassings in die sekondêre metabolisme van die harige wortels is deur middel van dunlaag chromatografie (DLC) en vloeibare chromatografie-massa spektroskopiese (VC-MS) analise ondersoek. Verskeie verbindings was in hoër vlakke in die harige wortels teenwoordig, as in die kontrole wortel ekstrakte. Transformasie het ook die kompleksiteit van die harige wortels se fitochemiese patroon verhoog, moontlik weens die produksie van nuwe verbindings of weens die opregulasie van bestaande metaboliese paaie. Die produksie van harige wortels en die vestiging daarvan in ‘n vloeibare sisteem tydens hierdie studie word beskou as ‘n belangrike stap na die opskalering van die kulture na bioreaktore. Hierdie wortels kan toekomstig tot die ontwikkeling van kulture met ‘n hoë produksie van gewenste metaboliete lei.
Hiraki, Simone Silva [UNESP]. "Sombreamento e aplicação de nitrogênio em plantas jovens de Jatropha curcas L." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/99882.
Full textFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O pinhão-manso (Jatropha curcas L.) é uma planta conhecida e cultivada na América desde tempos remotos, porém, somente nas últimas três décadas passou a ser estudado agronomicamente. É considerada uma espécie promissora no processo de produção de biocombustíveis devido ao seu elevado teor de óleo nas sementes. Por tratar-se de uma planta ainda em fase de domesticação, os estudos acerca de seu crescimento e fisiologia ainda são escassos, porém necessários para a expansão da cultura em todo o país, por isso, o objetivo desta pesquisa foi analisar o crescimento e o metabolismo do nitrogênio de plantas jovens de J. curcas cultivadas sob quatro condições de sombreamento (0, 30, 50 e 70 % de cobertura) e quatro concentrações de nitrato (0, 15, 30 e 45 mM). Durante 120 dias as plantas foram tratadas e avaliadas biometricamente quanto à altura, diâmetro, número de folhas, área foliar e massas fresca e seca. Fisiologicamente analisou-se a atividade das enzimas redutase do nitrato e glutamina sintetase, proteína total solúvel, análise quantitativa de nitrato e amônia. Os resultados obtidos sugerem que com relação ao crescimento inicial J. curcas é responsiva ao sombreamento, como características ecofisiológicas que se refletem em adaptações morfológicas de expansão da área foliar, alongamento do pecíolo e maior crescimento em altura buscando ambientes com maior luminosidade, sendo que ambientes com sombreamento de 30% são eficazes no controle da temperatura foliar e retenção de água no substrato permitindo o melhor desenvolvimento das plantas, entretanto, níveis acima de 50% de sombreamento são prejudiciais ao crescimento inicial da espécie por provocarem intenso estiolamento e redução no número médio de folhas. A realização da adubação nitrogenada é de suma importância...
The jatropha (Jatropha curcas L.) is a plant known in America and cultivated since ancient times, but only in the last three decades began to be studied agronomy. It is considered a promising species for the biofuels production because its high oil content in seeds. It‟s a plant still under domestication because it, studies on their growth and physiology are still scarce, but necessary to the expansion of culture throughout the country, so the objective of this research was to analyze the growth and nitrogen metabolism in young plants of J. curcas grown under four conditions of shading (0, 30, 50 and 70% coverage) and four nitrate concentrations (0, 15, 30 and 45 mM). For 120 days the plants were treated and biometrically evaluated as to height, diameter, number of leaves, leaf surface area and fresh and dry mass. Physiologically, we analyzed the enzymes activity of the nitrate reductase and glutamine synthetase, total soluble protein, quantitative analysis of nitrate and ammonia. The results suggest that with respect to the initial growth J. curcas is responsive to the shading, with physiologic characteristics that reflected in morphological adaptations of leaf area expansion, petiole elongation and greater height growth looking environments with higher brightness, and shading environments with 30% are effective in leaf temperature control and water retention in the substrate allowing for better development of plants, however, levels above 50% shading is detrimental to early growth of the species to cause intense etiolation and reduction in the number of leaves. The nitrogen fertilizer is a paramount importance in the early development of plants, the start deficiency visual symptoms appear in the first month after germination. Nitrate concentrations exceeding 30 mM are harmful because they cause a reduction... (Complete abstract click electronic access below)
Patto, Leonardo Silva. "Acrotonia da brotação de macieira em região de inverno ameno." Universidade Tecnológica Federal do Paraná, 2017. http://repositorio.utfpr.edu.br/jspui/handle/1/2287.
Full textDurante a dormência, as plantas de clima temperado cessam temporariamente o crescimento visível. No caso da macieira sabe-se que a temperatura é o fator ambiental que mais influencia a dormência. A planta necessita de baixas temperaturas para induzir a entrada e posteriormente a saída da dormência, para então retomar o crescimento vegetativo. Sabe-se que quando não ocorre acúmulo suficiente de frio, as plantas apresentam crescimento errático, com brotação ocorrendo predominantemente na porção distal dos ramos, comprometendo a brotação satisfatória. No entanto, há pouca informação sobre o metabolismo destes carboidratos e sua relação com a dormência de plantas em condições de inverno ameno. Contudo o objetivo deste trabalho foi avaliar as diferenças biológicas e bioquímicas entre as porções distal e proximal de ramos de macieira que influenciam a acrotonia. De abril a outubro dos anos de 2012, 2013 e 2014, foram coletados ramos e esporões de três cultivares de macieira (Eva, Galaxy e Fuji Suprema) cultivadas em um pomar comercial no município de Plamas-PR. Para avaliar a intensidade da dormência em gemas vegetativas foi realizado o teste de estacas de uma só gema, e o teste de Tabuenca para as gemas florais. Variáveis bioquímicas também foram avaliadas como o teor de proteínas solúveis; atividade de alfa-amilase; açúcares (glicose, frutose, sacarose e sorbitol); amido e umidade. De forma geral as cultivares apresentaram tempo médio de brotação (TMB) menores do que em clima temperado. A porcentagem de brotação manteve-se alta na maioria das coletas para as três cultivares. O teste de Tabuenca confirmou a precocidade da cultivar Eva e mostrou comportamento muito semelhante entre a ‘Galaxy’ e a ‘Fuji Suprema’. Além disso foi observado um intervalo muito curto entre a saída da endodormência em condições de forçagem e em condição de campo. A umidade ponderal variou conforme o padrão da temperatura em cada ano, sendo que em 2013, ano de maior acúmulo de frio a umidade ponderal apresentou os menores valores para todas as cultivares. Em 2013 a tendência de aumento da atividade da alfa-amilase e no teor de proteína coincidiram com o menor valor de TMB. A glicose e frutose apresentaram comportamento muito semelhantes entre si durante as observações. O teor de sacarose variou de ano para ano, não apresentando um padrão fixo. O sorbitol, açúcar mais abundante no xilema, tende a ser mais constante durante a dormência com leve tendência de redução próximo à brotação. O teor de amido tende a diminuir com os primeiros acúmulos de horas frio, no entanto frequentemente sofre ressíntese, devido às ondas de calor comuns no inverno. Existe diferenças entre as porções dital e proximal dos ramos para todas as variáveis analisadas, com teor de carboidrato, atividade enzimática, proteínas e umidade predominantemente maiores na porção distal dos ramos, com algumas exceções pontuais. Isso pode estar associado à ocorrência de acrotonia em macieiras. As plantas avaliadas parecem não entrar em dormência profunda em condição de inverno ameno, tendo seu metabolismo muito sujeito às oscilações de temperatura. O metabolismo das plantas tende a mudar de ano para ano de acordo com o padrão do inverno em cada ano.
During dormancy, temperate plants temporarily stops visible growth. In apple trees, it is known that temperature is the environmental factor that most influences dormancy. The plant requires low temperatures to induce input and then to output endodormancy, thus resumption vegetative grow. It is known that when there isn’t sufficient chilling accumulation, plants present erratic growth, with budbreak occurring predominantly in the distal portion of the branches, compromising the satisfactory budbreak. However, there is little information on the metabolism of these carbohydrates and their relationship to plant dormancy in subtropical humid climates. This work aimed to evaluate the biological and biochemical differences between the distal and proximal portions of apple tree branches that influence acrotonia. From April to October of 2012, 2013 and 2014, branches and spurs of three apple cultivars (Eva, Galaxy and Fuji Suprema) were harvested in a commercial orchard in Palmas-PR. In order to evaluate the dormancy intensity in vegetative and floral buds, single bud cuttings test and Tabuenca test were carried out, respectively. Biochemical variables were also evaluated such as soluble protein content; Alpha-amylase activity; Soluble sugars (glucose, fructose, sucrose and sorbitol); Starch and water content. In general, the cultivars showed the Mean Time to Budbrst (MTB) lower than in temperate climate. The budburst percentage remained high in most of the observation dates for the three cultivars. The Tabuenca test confirmed the precocity of the cultivar Eva and showed very similar behavior between 'Galaxy' and 'Fuji Suprema'. In addition, a very short interval between the endodormancy output under forcing conditions and in field condition was observed. The water content varied according to the temperature pattern in each year, and in 2013, the year of greatest cold accumulation, the lowest values for all cultivars were observed. In 2013, the trend of increased alpha-amylase activity and protein content coincided with the lower value of MTB. Glucose and fructose presented very similar behavior among themselves during the observations. The sucrose content varied from year to year and did not present a fixed pattern. Sorbitol, sugar most abundant in the xylem, tends to be more constant during dormancy with a slight reduction tendency close to budding. The starch content tends to decrease with the first chilling accumulations, however it often undergoes resynthesis due to the heat waves common in winter. There are differences between the distal and proximal portions of the branches for all variables analyzed, with carbohydrate content, enzymatic activity, proteins and humidity predominantly larger in the distal portion of the branches, with some occasional exceptions. This may be associated with the occurrence of acrotonia in apple trees. The evaluated apple trees do not appear to fall into deep endodormancy in a mild winter condition, their metabolism seems to be very subject to temperature oscillations. Plant metabolism tends to change from year to year according to the winter pattern in each year.
McCormick, Alistair James. "Expression behaviour of primary carbon metabolism genes during sugarcane culm development." Thesis, Stellenbosch : University of Stellenbosch, 2004. http://hdl.handle.net/10019.1/16387.
Full textENGLISH ABSTRACT: Despite numerous attempts involving a variety of target genes, the successful transgenic manipulation of sucrose accumulation in sugarcane remains elusive. It is becoming increasingly apparent that enhancing sucrose storage in the culm by molecular means may depend on the modification of the activity of a novel gene target. One possible approach to identify target genes playing crucial coarse regulatory roles in sucrose accumulation is to assess gene expression during the developmental transition of the culm from active growth to maturation. This study has resulted in the successful optimisation of a mRNA hybridisation technique to characterise the expression of 90 carbohydrate metabolism-related genes in three developmentally distinct regions of sugarcane culm. A further goal of this work was to extend the limited knowledge of the regulation of sucrose metabolism in sugarcane, as well as to complement existing data from physiological and biochemical studies. Three mRNA populations derived from the different culm regions were assayed and their hybridisation intensities to the immobilised gene sequences statistically evaluated. The relative mRNA transcript abundance of 74 genes from three differing regions of culm maturity was documented. Genes exhibiting high relative expression in the culm included aldolase, hexokinase, cellulase, alcohol dehydrogenase and soluble acid invertase. Several genes (15) were demonstrated to have significantly different expression levels in the culm regions assessed. These included UDP-glucose pyrophosphorylase and UDP-glucose dehydrogenase, which were down-regulated between immature and mature internodes. Conversely, sucrose phosphate synthase, sucrose synthase and neutral invertase exhibited up-regulation in maturing internodal tissue. A variety of sugar transporters were also found to be up-regulated in mature culm, indicating a possible control point of flux into mature stem sink tissues. Combined with knowledge of the levels of key metabolites and metabolic intermediates this gene expression data will contribute to identifying key control points of sucrose accumulation in sugarcane and assist in the identification of gene targets for future manipulation by transgenic approaches.
AFRIKAANSE OPSOMMING: Ondanks verskeie pogings, waartydens verskeie gene geteiken is, is daar nog weinig sukses behaal om sukrose-akkumulering te verhoog. Toenemend wil dit voorkom asof suksesvolle genetiese manipulering van sukroseberging in die stingel van die verandering van ‘n nuwe geen afhanklik sal wees. Een van die moontlike benaderings wat gevolg kan word om potensiële teiken gene wat ‘n belangrike rol in die beheer van sukrose-opberging speel te identifiseer, is om geen uitdrukkingspatrone in die stingel tydens die omskakeling van aktiewe groei tot volwassenheid te karakteriseer. In hierdie studie is ‘n metode gebaseer op die hibridisering van mRNA geoptimiseer en suksesvol aangewend om die uitdrukkingspatrone van 90 verskillende geselekteerde gene, wat vir sleutelensieme in die beheer van koolhidraatmetabolisme kodeer, te bestudeer. Die doel met die ondersoek was om die beperkte kennis oor die regulering van koolhidraatmetabolisme uit te brei en om die bestaande inligting afgelei van fisiologiese en biochemiese-studies aan te vul. Drie verskillende mRNApopulasies, verkry uit verskillende dele van die stingel, is ontleed deur verskillende peilers te gebruik. Die gegewens is statisties ontleed en dit het afleidings oor die verandering in uitdrukking van hierdie gene moontlik gemaak. Die relatiewe konsentrasies van 74 verskillende gene is gedokumenteer. Gene wat sterk uitgedruk word het aldolase, heksokinase, sellulase, alkoholdehidrogenase en ongebonde suurinvertase ingesluit. Die uitdrukkingspatrone van 15 gene het tussen die verkillende weefsels gevarieer. Gene waarvan die uitdrukking tydens die oorgang na volwassenheid verlaag sluit in UDP-glukose pirofosforilase en UDP-glukose dehidrogenase en waarvan die uitdrukking verhoog sukrosefosfaatsintase, sukrosesintase en neutrale invertase in. Die uitdrukking van verskeie suikertransporter gene verhoog tydens volwassewording. Hierdie inligting te same met die huidige kennis oor heersende metabolietvlakke sal bydrae tot die identifisering van geenteikens vir toekomstige genetiese manupulering.
Edwards, E. Anne. "Characterisation of glutathione reductase from Pisum sativum L." Thesis, University of East Anglia, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278197.
Full textBreitkreuz, Kevin Edmund. "Subcellular localization of gamma-aminobutyrate metabolism and transport of gamma-aminobutyrate in plants." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0004/NQ40365.pdf.
Full textHiraki, Simone Silva. "Sombreamento e aplicação de nitrogênio em plantas jovens de Jatropha curcas L. /." Ilha Solteira : [s.n.], 2011. http://hdl.handle.net/11449/99882.
Full textBanca: Liliane Santos de Camargos
Banca: Enio Tiago de Oliveira
Resumo: O pinhão-manso (Jatropha curcas L.) é uma planta conhecida e cultivada na América desde tempos remotos, porém, somente nas últimas três décadas passou a ser estudado agronomicamente. É considerada uma espécie promissora no processo de produção de biocombustíveis devido ao seu elevado teor de óleo nas sementes. Por tratar-se de uma planta ainda em fase de domesticação, os estudos acerca de seu crescimento e fisiologia ainda são escassos, porém necessários para a expansão da cultura em todo o país, por isso, o objetivo desta pesquisa foi analisar o crescimento e o metabolismo do nitrogênio de plantas jovens de J. curcas cultivadas sob quatro condições de sombreamento (0, 30, 50 e 70 % de cobertura) e quatro concentrações de nitrato (0, 15, 30 e 45 mM). Durante 120 dias as plantas foram tratadas e avaliadas biometricamente quanto à altura, diâmetro, número de folhas, área foliar e massas fresca e seca. Fisiologicamente analisou-se a atividade das enzimas redutase do nitrato e glutamina sintetase, proteína total solúvel, análise quantitativa de nitrato e amônia. Os resultados obtidos sugerem que com relação ao crescimento inicial J. curcas é responsiva ao sombreamento, como características ecofisiológicas que se refletem em adaptações morfológicas de expansão da área foliar, alongamento do pecíolo e maior crescimento em altura buscando ambientes com maior luminosidade, sendo que ambientes com sombreamento de 30% são eficazes no controle da temperatura foliar e retenção de água no substrato permitindo o melhor desenvolvimento das plantas, entretanto, níveis acima de 50% de sombreamento são prejudiciais ao crescimento inicial da espécie por provocarem intenso estiolamento e redução no número médio de folhas. A realização da adubação nitrogenada é de suma importância... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The jatropha (Jatropha curcas L.) is a plant known in America and cultivated since ancient times, but only in the last three decades began to be studied agronomy. It is considered a promising species for the biofuels production because its high oil content in seeds. It‟s a plant still under domestication because it, studies on their growth and physiology are still scarce, but necessary to the expansion of culture throughout the country, so the objective of this research was to analyze the growth and nitrogen metabolism in young plants of J. curcas grown under four conditions of shading (0, 30, 50 and 70% coverage) and four nitrate concentrations (0, 15, 30 and 45 mM). For 120 days the plants were treated and biometrically evaluated as to height, diameter, number of leaves, leaf surface area and fresh and dry mass. Physiologically, we analyzed the enzymes activity of the nitrate reductase and glutamine synthetase, total soluble protein, quantitative analysis of nitrate and ammonia. The results suggest that with respect to the initial growth J. curcas is responsive to the shading, with physiologic characteristics that reflected in morphological adaptations of leaf area expansion, petiole elongation and greater height growth looking environments with higher brightness, and shading environments with 30% are effective in leaf temperature control and water retention in the substrate allowing for better development of plants, however, levels above 50% shading is detrimental to early growth of the species to cause intense etiolation and reduction in the number of leaves. The nitrogen fertilizer is a paramount importance in the early development of plants, the start deficiency visual symptoms appear in the first month after germination. Nitrate concentrations exceeding 30 mM are harmful because they cause a reduction... (Complete abstract click electronic access below)
Zheng, Youbin. "An in vivo analysis of the impact of ozone on primary carbon metabolism." Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388732.
Full textMASON, HUGH STANLEY. "ALTERATIONS IN POLYRIBOSOME AND MESSENGER RIBONUCLEIC ACID METABOLISM AND MESSENGER RIBONUCLEOPROTEIN UTILIZATION IN OSMOTICALLY STRESSED PLANT SEEDLINGS (WATER STATUS, GROWTH, HORDEUM VULGARE)." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/188155.
Full textHawker, John Seth. "Sucrose and starch metabolism in leaves, storage organs and developing fruits of higher plants." Title page, contents and summary only, 1988. http://web4.library.adelaide.edu.au/theses/09SD/09sdh392.pdf.
Full textHenne, Melina [Verfasser]. "Investigation of the role of sulfurtransferases in the metabolism of higher plants / Melina Henne." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2013. http://d-nb.info/1043713581/34.
Full textFisk, Stuart. "The effect of increased plastid transketolase activity on thiamine metabolism in transgenic tobacco plants." Thesis, University of Essex, 2015. http://repository.essex.ac.uk/15461/.
Full textJuvany, Cánovas Marta. "Edat cronològica, edat fisiològica i sexe: factors determinants de l'estrès oxidatiu en plantes." Doctoral thesis, Universitat de Barcelona, 2014. http://hdl.handle.net/10803/283933.
Full textMetabolic processes in plants such as photosynthesis, photorespiration and respiration, produce reactive oxygen species (ROS). ROS are highly toxic molecules but besides of its damaging nature they are implicated in cell signaling in different cellular processes. However, under stress conditions plants can suffer an increase of ROS levels. When ROS concentration becomes high enough to overwhelm antioxidant systems, plant suffer oxidative stress as a consequence of the unbalanced cellular redox status. Despite the importance to unravel plant stress responses, little is known about the mechanisms implicated in perennial plants. Furthermore, the possible effect of plant intrinsic factors, as plant age or reproductive effort, in oxidative stress levels is still poorly understood. The main objective of this thesis was to determine how plant age, both chronological and physiological, as well as reproductive effort may influence oxidative stress levels in perennial plants. With this purpose levels of antioxidants as carotenoids, anthocyanins and alpha-tocopherol together with endogenous contents of stress hormones were measured, but with special emphasis in malondialdehyde acid levels, a byproduct of lipid peroxidation. To better understand the plant age effect we used leaves and juvenile plants of Pistacia lentiscus as well as moribund beech trees. Increases in lipid peroxidation not only could mean an oxidative damage but play a signaling role. Therefore, chronolorgical age concomitantly with the measure of oxidative stress levels is a good indicator of plant physiological age. Sex-related changes in photoprotection mechanisms between female and male plants of Pistacia lentiscus, a dioecious plant, where observed under climatological adverse conditions. Although females phowed higher oxidative stress levels compared to males, females were not affected negatively, suggesting a role in signaling. In addition, photoprotection capacity was higher in reproductive shoots relative to non-reproductive shoots in females, thus suggesting that females prioritized protection to fruit-bearing shoots.
Wiese, Anna Johanna. "Comparative analyses of primary carbon metabolism in parasitic plant species." Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/85740.
Full textENGLISH ABSTRACT: Most terrestrial plants make use of beneficial symbiotic associations to obtain nutrients (eg. nitrogen (N) and phosphorous (P)) from fungi in exchange for photoautotrophic carbon. However, plant parasitism (defined here as the ability of certain plants to parasitize other living material) has evolved in the plant kingdom and such plants obtain some, or all, of their nutritional needs from a host, which is severely negatively impacted by the parasite. While the physiological adaptations are well studied, the underlying molecular and biochemical mechanisms of plant parasitism remain largely unknown. As a first approach, a biochemical blueprint of primary metabolites present within parasitic plant species was constructed. The metabolomes of nineteen parasitic plants, ranging from hemi- and holoparasitism to mycoheterotrophism, were profiled via gas chromatography mass spectrometry (GC MS) based technology and targeted spectrophotometric assays. Based on these analyses, three important observations were made. First, parasitic plants were severely carbon deprived, despite being successful in colonizing and exploiting their hosts. Second, the levels of organic acids participating in mitochondrial respiration decreased and certain amino acids and soluble protein content increased. This suggests that parasitic plants utilize alternative respiratory substrates to compensate for a limitation in carbon supply. Third, although characterized by reduced carbohydrate pools, minor sugars normally not associated with plant metabolism, dominated the soluble sugar pool. The presence and significance of one of these sugars, namely turanose (α-D-glucopyranosyl-(1→3)-α-D-fructofuranose), was further investigated. Turanose biosynthetic reactions could be demonstrated in Orobanche minor extracts. Protein purification and mass spectrometry identification suggested that turanose biosynthesis occurred uniquely in parasitic plants. Future work will elucidate the functional significance of turanose metabolism in plant parasitism. Taken together, this study significantly contributes to our understanding of plant parasitism through development of metabolic signatures associated with distinct parasitic classes. These biochemical profiles highlighted several important strategies and alternative metabolic pathways that are either expressed or constitutively activated during parasitism. This knowledge broadens the scope of using parasitic plants in several biotechnological applications or as a novel research tool to address fundamental questions in plant science.
AFRIKAANSE OPSOMMING: Meeste landelike plante maak gebruik van voordelige simbiotiese assosiasies met swamme om voedinsgtowwe (bv. stikstof (N) en fosfor (P)) van hulle te verkry in ruil vir koolstof geproduseer deur die plant. Plant parasitisme (gedefinieer hier as die vermoë van sekere plante om ander lewende materiaal te parasiteer) het ontwikkel in die planteryk waar hulle sommige, of al hul voedings stowwe van 'n gasheer plant ontvang, wat erg negatief geraak word deur die parasiet. Terwyl die fisiologiese aanpassings goed gebestudeer is, is die onderliggende molekulêre en biochemiese meganismes van plant parasitisme steeds grootliks onbekend. As 'n eerste benadering, was hierdie projek geïnisieer om 'n biochemiese bloudruk op te bou van primêre metaboliete teenwoordig in parasitiese plante. Die metabolome van negentien parasitiese spesies, wat wissel van hemi - en holoparasiete tot mikoheterotrofiese plante, is ondersoek deur gas chromatografie – massa spektrometrie (GC MS) gebaseerde tegnologie en geteikende spektrofotometriese toetse. Gebaseer op hierdie ontledings was drie belangrike waarnemings gemaak. Eerstens, parasitiese plante was erg koolstof arm, ten spyte daarvan dat hulle suksesvol is in die aanhegting en ontginning van voedingstowwe vanaf gasheer plante. Tweedens, die vlakke van organiese sure wat deelneem aan mitochondriale respirasie het afgeneem, terwyl sekere aminosure en oplosbare proteïen inhoude toegeneem het. Dit dui daarop dat parasitiese plante gebruik maak van alternatiewe respiratoriese substrate om te vergoed vir 'n beperking in koolstof aanbod. Derde, alhoewel parasitiese plante gekenmerk word deur verminderde koolhidraat inhoude, het skaarse suikers wat normaalweg nie verband hou met plant metabolisme nie, hulle oplosbare suiker inhoud oorheers. Die teenwoordigheid en betekenis van een van hierdie suikers, naamlik turanose (α -D -glucopyranosyl-(1→3)-α-D-fructofuranose), was verder ondersoek. Die sintese reaksie van turanose kan gedemonstreer word in Orobanche hederae uittreksels. Proteïen suiwering en massa spektrometrie identifikasie het voorgestel dat turanose biosintese uniek plaasvind in parasitiese plante. Toekomstige werk sal aandui wat die betekenis is van turanose metabolisme in plant parasitisme. Saamgevat het hierdie studie aansienlik bygedra tot ons begrip van plant parasitisme deur ontwikkeling van metaboliese handtekeninge wat verband hou met onderskeie parasitiese klasse. Hierdie biochemiese profiele beklemtoon verskeie belangrike strategieë en alternatiewe metaboliese paaie wat óf uitgedruk of konstitutief geaktiveer word tydens parasitisme. Hierdie kennis verbreed die omvang van die gebruik van parasitiese plante in verskeie biotegnologiese toepassings of as 'n nuwe navorsings instrument om fundamentele vrae in plant wetenskap aan te spreek.