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Journal articles on the topic 'Plant tissue culture'

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Published: 4 June 2021
Last updated: 30 July 2024

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1

Prakash, Jitendra. "Plant Tissue Culture." Nature Biotechnology 9, no. 7 (July 1991): 607. http://dx.doi.org/10.1038/nbt0791-607.

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2

Dagla, H. R. "Plant tissue culture." Resonance 17, no. 8 (August 2012): 759–67. http://dx.doi.org/10.1007/s12045-012-0086-8.

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3

Illg, Rolf Dieter. "Plant tissue culture techniques." Memórias do Instituto Oswaldo Cruz 86, suppl 2 (1991): 21–24. http://dx.doi.org/10.1590/s0074-02761991000600008.

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4

J. Robins, Ichard. "Plant tissue culture manual." Phytochemistry 31, no. 9 (September 1992): 3301–2. http://dx.doi.org/10.1016/0031-9422(92)83507-u.

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5

Niazian, M., S. A. Sadat Noori, P. Galuszka, and S. M. M. Mortazavian. "Tissue culture-based Agrobacterium-mediated and in planta transformation methods." Czech Journal of Genetics and Plant Breeding 53, No. 4 (November 10, 2017): 133–43. http://dx.doi.org/10.17221/177/2016-cjgpb.

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Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. Development of genotype-independent gene transformation method is of great interest in many plants. Some tissue culture-independent Agrobacterium-mediated gene transformation methods are reported in individual plants and crops. Generally, these methods are called in planta gene transformation. In planta transformation methods are free from somaclonal variation and easier, quicker, and simpler than tissue culture-based transformation methods. Vacuum infiltration, injection of Agrobacterium culture to plant tissues, pollen-tube pathway, floral dip and floral spray are the main methods of in planta transformation. Each of these methods has its own advantages and disadvantages. Simplicity and reliability are the primary reasons for the popularity of the in planta methods. These methods are much quicker than regular tissue culture-based Agrobacterium-mediated gene transformation and success can be achieved by non-experts. In the present review, we highlight all methods of in planta transformation comparing them with regular tissue culture-based Agrobacterium-mediated transformation methods and then recently successful transformations using these methods are presented.
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6

B.M. Johri, P.S. Srivastava, and Madhumati Purohit. "Plant tissue culture and biotechnology." Journal of Palaeosciences 46, no. 3 (December 31, 1997): 134–59. http://dx.doi.org/10.54991/jop.1997.1357.

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Plant tissue culture has progressed steadily ever since its inception in 1902. The initial experiments related to various tissues that could sustain prolonged in vitro conditions. The differential response of the cultured tissues under variable chemical milieu provided the necessary impetus to utilize the technique in a profitable manner. Over the years efficacy of the technique became apparent when noticeable in vitro morphogenic responses could be used to unravel the mysteries of growth and differentiation. Expectedly, therefore, any morphogenic event expressed in vitro could be correlated to the specific components of the nutritive medium. By the 1970s the applicability of the technique came to be realized with the possibility of exploring somatic hybridization, micropropagation of recalcitrant species, haploid, and triploid plants, and finally genetic manipulations. Today, plant tissue culture has become an integral part of biotechnology and is being routinely employed for the improvement of crops and legumes- the backbone of human nutrition that can also aid in the amelioration of malnutrition of millions of sufferers. The ultimate success with the transfer of 'nif’-gene to non-leguminous plants would help save millions of dollars in chemical fertilizers which can then be profitably used for the welfare of the human race.
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7

Hain, Patricia, and Donald Lee. "Transformation 1: Plant Tissue Culture." Journal of Natural Resources and Life Sciences Education 32, no. 1 (2003): 135. http://dx.doi.org/10.2134/jnrlse.2003.0135b.

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8

Fay, Michael, D. J. Donnelly, W. E. Vidaver, and T. R. Dudley. "Glossary of Plant Tissue Culture." Kew Bulletin 45, no. 2 (1990): 386. http://dx.doi.org/10.2307/4115705.

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9

Gamborg, Oluf L. "Plant tissue culture. Biotechnology. Milestones." In Vitro Cellular & Developmental Biology - Plant 38, no. 2 (March 2002): 84–92. http://dx.doi.org/10.1079/ivp2001281.

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10

Hahne, Günther. "Glossary of plant tissue culture." Plant Science 60, no. 1 (January 1989): 145–46. http://dx.doi.org/10.1016/0168-9452(89)90056-3.

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11

Vasil, I. K. "Plant cell and tissue culture." Plant Science 71, no. 2 (January 1990): 278. http://dx.doi.org/10.1016/0168-9452(90)90019-k.

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12

Scoles, Graham. "Plant cell and tissue culture." Trends in Biotechnology 10 (1992): 221. http://dx.doi.org/10.1016/0167-7799(92)90219-l.

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13

Teixeira da Silva, Jaime A., Judit Dobránszki, and Silvia Ross. "Phloroglucinol in plant tissue culture." In Vitro Cellular & Developmental Biology - Plant 49, no. 1 (January 26, 2013): 1–16. http://dx.doi.org/10.1007/s11627-013-9491-2.

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14

Johnson, Medeline I. "New plant tissue culture collection." Plant Molecular Biology Reporter 3, no. 1-2 (March 1985): 92. http://dx.doi.org/10.1007/bf02994729.

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15

Evans, David A. "Plant cell and tissue culture." Trends in Genetics 8, no. 2 (February 1992): 78. http://dx.doi.org/10.1016/0168-9525(92)90357-a.

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16

Jones, O. P. "Plant propagation by tissue culture." Scientia Horticulturae 27, no. 3-4 (December 1985): 350–51. http://dx.doi.org/10.1016/0304-4238(85)90041-x.

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17

Pierik, R. L. M. "Experiments in plant tissue culture." Scientia Horticulturae 30, no. 1-2 (November 1986): 162–63. http://dx.doi.org/10.1016/0304-4238(86)90094-4.

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18

Smulders, M. J. M., and G. J. de Klerk. "Epigenetics in plant tissue culture." Plant Growth Regulation 63, no. 2 (October 8, 2010): 137–46. http://dx.doi.org/10.1007/s10725-010-9531-4.

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19

Smith, Roberta H. "Dictionary of Plant Tissue Culture." Journal of Environmental Quality 36, no. 6 (November 2007): 1928. http://dx.doi.org/10.2134/jeq2007.0019br.

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20

FURUYA, TSUTOMU. "Plant Tissue Culture in Biotechnology." YAKUGAKU ZASSHI 106, no. 10 (1986): 856–66. http://dx.doi.org/10.1248/yakushi1947.106.10_856.

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21

Polivanova, Oksana B., and Vladislav A. Bedarev. "Hyperhydricity in Plant Tissue Culture." Plants 11, no. 23 (November 30, 2022): 3313. http://dx.doi.org/10.3390/plants11233313.

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Hyperhydricity is the most common physiological disorder in in vitro plant cultivation. It is characterized by certain anatomical, morphological, physiological, and metabolic disturbances. Hyperhydricity significantly complicates the use of cell and tissue culture in research, reduces the efficiency of clonal micropropagation and the quality of seedlings, prevents the adaptation of plants in vivo, and can lead to significant losses of plant material. This review considers the main symptoms and causes of hyperhydricity, such as oxidative stress, impaired nitrogen metabolism, and the imbalance of endogenous hormones. The main factors influencing the level of hyperhydricity of plants in vitro are the mineral and hormonal composition of a medium and cultivation conditions, in particular the aeration of cultivation vessels. Based on these factors, various approaches are proposed to eliminate hyperhydricity, such as varying the mineral and hormonal composition of the medium, the use of exogenous additives, aeration systems, and specific lighting. However, not all methods used are universal in eliminating the symptoms of hyperhydricity. Therefore, the study of hyperhydricity requires a comprehensive approach, and measures aimed at its elimination should be complex and species-specific.
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22

Bhojwani, Sant S. "Glossary of plant tissue culture." Scientia Horticulturae 41, no. 1-2 (December 1989): 171. http://dx.doi.org/10.1016/0304-4238(89)90061-7.

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23

Heinhorst, Sabine. "Plant cell and tissue culture." Analytical Biochemistry 192, no. 2 (February 1991): 459. http://dx.doi.org/10.1016/0003-2697(91)90567-d.

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24

Thorpe, Trevor A. "History of plant tissue culture." Molecular Biotechnology 37, no. 2 (June 27, 2007): 169–80. http://dx.doi.org/10.1007/s12033-007-0031-3.

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25

Thakur, Shivani, Shruti, Sohin Hashmi, Saket Mishra, Shashi Kant Ekka, Akhilesh Kushwaha, and Reena Kujur. "A Review on Plant Tissue Culture." Asian Journal of Biology 20, no. 2 (February 15, 2024): 14–18. http://dx.doi.org/10.9734/ajob/2024/v20i2387.

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Plant tissue and cell culture involve the aseptic cultivation and propagation of plant cells, tissues, and organs in a meticulously controlled laboratory setting. This innovative method harnesses the potential of nutrient-rich media to efficiently replicate plant cells on a large scale, resulting in the rapid production of mature and disease-free plants. The cornerstone of commercial technology in this field is micropropagation, a process that achieves swift proliferation from minute plant cuttings, axillary buds, and, to a limited extent, from somatic embryos and cell clumps in suspension cultures and bioreactors. Micropropagation, a pivotal aspect of plant tissue and cell culture, holds immense value in generating high-quality and consistent planting materials. These materials find applications across diverse fields, including molecular genetic engineering, plant breeding, horticulture production, and environmental preservation. The process of micropopagation unfolds through several distinct stages, including propagation, subculture of explants for proliferation, shooting and rooting, and hardening. These stages collectively form a universal framework for large-scale multiplication of plants. This technique plays a critical role in overcoming the limitations of traditional plant propagation methods. By allowing for controlled and accelerated growth in a laboratory environment, plant tissue and cell culture contribute to the efficient production of disease-resistant and genetically uniform plant materials. This not only supports advancements in various scientific disciplines but also addresses practical needs in agriculture, horticulture, and environmental conservation. The continuous refinement and application of plant tissue and cell culture methods underscore their significance in meeting the growing demands for sustainable and high-quality plant materials across diverse sectors.
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26

Anushi, Shubham Jain, Manjunath Rathod, Gopa Mishra, V. Lakshmi Prasanna Kumari, Hari Baksh, Saransh Saxena, and Lalu Prasad. "Plant Tissue Culture for Medical Therapy: Unlocking the Potential of Medicinal Plants." Current Journal of Applied Science and Technology 42, no. 46 (December 1, 2023): 7–22. http://dx.doi.org/10.9734/cjast/2023/v42i464289.

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Plant tissue culture is emerging as a pivotal biotechnological tool with profound implications for medical therapy, particularly in the realm of herbal medicine. Medicinal plants have long been cherished for their natural healing properties. However, escalating demand, habitat destruction, and overharvesting have threatened the availability and sustainability of these valuable resources. Plant tissue culture addresses these concerns by enabling the mass propagation of medicinal plants. In controlled environments, plant tissues can be multiplied rapidly, providing a continuous and sustainable source of plant material. This not only safeguards wild populations but also ensures a consistent supply of bioactive compounds that form the basis of herbal therapies. One of the most transformative applications of plant tissue culture in medical therapy is the manipulation of secondary metabolite production. Medicinal plants synthesize a diverse array of bioactive compounds, such as alkaloids, flavonoids, and terpenoids, with therapeutic properties. Through precise control of growth conditions and genetic modification, plant tissue culture can enhance the yield of these compounds, thereby increasing the potency and efficacy of herbal medicines. This precision is instrumental in the pharmaceutical industry, where the isolation and production of specific bioactive compounds can lead to the development of novel drugs and therapies. In addition to bolstering yields, plant tissue culture offers the advantage of disease-free plant material. By maintaining cultures in sterile conditions, the risk of contaminants and pathogens is mitigated, enhancing the safety and quality of herbal medicines. These cultures can also serve as a continuous source of plant-derived compounds, enabling a consistent supply of bioactive substances. Furthermore, plant tissue culture is a crucial tool for research and development in the field of medicinal plants. It provides a controlled platform for studying plant biology, optimizing growth conditions, and investigating the mechanisms underlying secondary metabolite production. These insights contribute to the development of improved plant varieties with enhanced medicinal properties, addressing the evolving needs of medical therapy. While the potential of plant tissue culture in medical therapy is vast, it is essential to underscore the importance of rigorous research, quality control, and safety assessments. Ensuring the safety and efficacy of products derived from tissue-cultured plants is paramount to their acceptance and use in medical applications. Compliance with regulatory standards and collaboration with healthcare professionals are integral to upholding the quality and safety of medicinal products.
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27

Wightman, Raymond, and C. J. Luo. "From mammalian tissue engineering to 3D plant cell culture." Biochemist 38, no. 4 (August 1, 2016): 32–35. http://dx.doi.org/10.1042/bio03804032.

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Recent advances applying mammalian tissue engineering to in vitro plant cell culture have successfully cultured single plant cells in a 3D microstructure, leading to the discovery of plant cell behaviours that were previously not envisaged. Animal and plant cells share a number of properties that rely on a hierarchical microenvironment for creating complex tissues. Both mammalian tissue engineering and 3D plant culture employ tailored scaffolds that alter a cell's behaviour from the initial culture used for seeding. For humans, these techniques are revolutionizing healthcare strategies, particularly in regenerative medicine and cancer studies. For plants, we predict applications both in fundamental research to study morphogenesis and for synthetic biology in the agri-biotech sector.
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28

Poronnik, О. О. "OBTAINING OF PLANT TISSUE CULTURE Scutellaria baicalensis GEORGI. AND ITS BIOCHEMICAL ANALYSIS." Biotechnologia Acta 14, no. 6 (December 2021): 53–58. http://dx.doi.org/10.15407/biotech14.06.0053.

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Aim. To obtain a plant tissue culture of S. baicalensis as a possible source of biologically active compounds (BAC) with a wide range of pharmacological action. Methods. Plant tissue culture, photocolorimetric method, reversed-phase high performance liquid chromatography (HPLC) method. Results. Two stably productive plant tissue culture strains (16SB3 and 20SB4) of S. baicalensis were obtained from fragments of roots seedling on a specially developed agar nutrient medium 5С01. The yield of dry biomass from 1 liter of this medium per passage (21st day of growth) for strain 16SB3 is 25–30 g, for strain 20SB4 – 30–40 g. The total content of flavonoids in dry biomass was in terms of routine for strains 16SB3 and 20SB4 – 0.6–0.9 and 0.7–0.9 mg/g, respectively, and the yield of flavonoids – 18–27 and 21–36 mg/l of nutrient medium, respectively. BAC typical for plants in nature, in particular, flavonoids vogonin, baikalein, neobaikalein, skulkapfavon and their derivatives, were found in the studied biomass of both strains. Conclusions. It was found that the biomass of the two strains of S. baicalensis plant tissue culture accumulated the same BAC, in particular, flavonoids, as do plants in natural conditions. The resulting plant tissue culture is promising as a possible source of Baikal skullcap BAC.
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29

Onay, Ahmet, Hakan Yildirim, Yelda Ozden Tokatli, Hulya Akdemir, and Veysel Suzerer. "Plant tissue culture techniques—Tools in plant micropropagation." Current Opinion in Biotechnology 22 (September 2011): S130. http://dx.doi.org/10.1016/j.copbio.2011.05.426.

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30

Kunakh, V. A., D. O. Navrotska, M. O. Twardovska, and I. O. Andreev. "Peculiarities of chromosomal variability in cultured tissues of Deschampsia antarctica Desv. plants with different chromosome numbers." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 14, no. 1 (June 20, 2016): 36–43. http://dx.doi.org/10.7124/visnyk.utgis.14.1.542.

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Aim. To clarify the details of chromosome variation in calli derived from D. antarctica plants in the initial passages of the culture in vitro. Methods. Induction of callus from root explants of plants, which were grown from seeds, and consequent subcultivation of tissue culture. Cytogenetic analysis of squashed slides stained by acetic-orcein and counting the number of chromosomes in mitotic metaphase plates. Results. There were analyzed the cultured tissues derived from D. antarctica plants with different chromosome numbers: diploid plants (2n=26), mixoploid plant with B-chromosomes (2n=26+1-3B), and mixoploid plant with near-triploid modal class (2n=36, 38). Analysis of callus tissues of all plants at 2-4 passages revealed mixoploidy, presence of polyploid and aneuploid cells. The modal class in all studied calli was composed of diploid and aneuploid cells with near-diploid chromosome number. The cytogenetic structure of cell population of cultured tissues was found to vary with characteristics of the karyotype of donor plant. The largest range of variation in the number of chromosomes (from 18 to 63 chromosomes) was found in tissue culture of diploid plant (2n=26) from the Galindez Island, and the highest frequencies of polyploid (47 %) and aneuploid cells were in the culture of mixoploid plant with near-triploid modal class from the Big Yalour Island. Conclusions. In different D. antarctica cultured tissues at the early stages of the culture, the modal class was composed of diploid cells and cells with near-diploid chromosome number irrespective of karyotype of donor plant (diploid, mixoploid poliploid).Key words: Deschampsia antarctica Desv., plant tissue culture, chromosomal variability in vitro, mixoploidy.
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31

Tisserat, Brent. "Growth Responses and Construction Costs of Various Tissue Culture Systems." HortTechnology 6, no. 1 (January 1996): 62–68. http://dx.doi.org/10.21273/horttech.6.1.62.

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The influence of the culture chamber size and medium volume on the growth rates of shoot tips of peas, lettuce, kidney beans, and spearmint were determined after 8 weeks of incubation. Cultures were grown in a variety of culture chambers including culture tubes, baby food jars, Magenta GA-7 containers, 1-pint Mason jars, 1-quart Mason jars used with and without an automated plant culture system (APCS), 0.5-gal Mason jars with and without an APCS, Bio-safe chambers with an APCS, and polycarbonate culture chambers with an APCS having culture chamber volumes of 55, 143, 365, 462, 925, 1850, 6000, and 16,400 ml, respectively. Plans are presented for the construction of various culture chambers used in an APCS. The APCS consisted of a peristaltic pump, media reservoir containing 1 liter of liquid nutrient medium, and a culture chamber. Cultures grown with an APCS consistently produced higher fresh weights than cultures using any of the agar culture systems tested. Growth rates varied considerably depending on the plant species and culture system tested. Peas, lettuce, and spearmint exhibited flowering only when grown in the APCS. A cost comparison using the APCS versus various conventional tissue culture systems is presented.
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32

Beck, Mike. "Plant Tissue Culture in a Bag." American Biology Teacher 62, no. 9 (November 1, 2000): 652–53. http://dx.doi.org/10.2307/4451004.

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33

INOGUCHI, Masahiko, and Hiroshi HARADA. "Historical Steps in Plant Tissue Culture." Plant tissue culture letters 2, no. 1 (1985): 12–13. http://dx.doi.org/10.5511/plantbiotechnology1984.2.12.

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34

SATO, Makoto, Masanori KUROYANAGI, Akira UENO, Koichiro SHIMOMURA, and Motoyoshi SATAKE. "Plant Tissue Culture of Zingiberaceae (I)." Plant tissue culture letters 4, no. 2 (1987): 82–85. http://dx.doi.org/10.5511/plantbiotechnology1984.4.82.

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35

SATO, Makoto, Masanori KUROYANAGI, Akira UENO, Koichiro SHIMOMURA, and Motoyoshi SATAKE. "Plant Tissue Culture of Zingiberaceae (II)." Plant tissue culture letters 4, no. 2 (1987): 86–89. http://dx.doi.org/10.5511/plantbiotechnology1984.4.86.

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36

Cheema, H. K. "PLANT TISSUE CULTURE EDUCATION IN INDIA." Acta Horticulturae, no. 350 (November 1993): 265–66. http://dx.doi.org/10.17660/actahortic.1993.350.41.

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37

Beck, Mike. "Plant Tissue Culture in a Bag." American Biology Teacher 62, no. 9 (November 2000): 652–53. http://dx.doi.org/10.1662/0002-7685(2000)062[0652:ptciab]2.0.co;2.

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38

Lakhera, Kanchan, Amit Kumar, Anju Rani, Rekha Dixit, and Sonali Rana. "Plant tissue culture and its application." Bulletin of Pure & Applied Sciences- Botany 37b, no. 1 (2018): 32. http://dx.doi.org/10.5958/2320-3196.2018.00004.6.

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39

Hill, Kristine, and G. Eric Schaller. "Enhancing plant regeneration in tissue culture." Plant Signaling & Behavior 8, no. 10 (October 2013): e25709. http://dx.doi.org/10.4161/psb.25709.

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40

Fuller, Michael P., and Frances M. Fuller. "Plant tissue culture using Brassico seedlings." Journal of Biological Education 29, no. 1 (March 1995): 53–59. http://dx.doi.org/10.1080/00219266.1995.9655419.

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41

Ellis, B. E. "Natural products from plant tissue culture." Natural Product Reports 5, no. 6 (1988): 581. http://dx.doi.org/10.1039/np9880500581.

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42

Hahne, Günther. "Plant tissue culture: Application and limitations." Plant Science 77, no. 1 (January 1991): 137. http://dx.doi.org/10.1016/0168-9452(91)90190-j.

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43

Chen, Chiachung. "Humidity in Plant Tissue Culture Vessels." Biosystems Engineering 88, no. 2 (June 2004): 231–41. http://dx.doi.org/10.1016/j.biosystemseng.2004.02.007.

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44

Pierik, R. L. M. "Plant tissue culture: A classified bibliography." Scientia Horticulturae 34, no. 3-4 (February 1988): 312–14. http://dx.doi.org/10.1016/0304-4238(88)90105-7.

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45

Zárský, V. "Plant Tissue Culture: Theory and Practice." Biologia Plantarum 27, no. 1 (January 1985): 73. http://dx.doi.org/10.1007/bf02894638.

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46

Harborne, Jeffrey B. "Secondary products from plant tissue culture." Phytochemistry 30, no. 10 (January 1991): 3500–3501. http://dx.doi.org/10.1016/0031-9422(91)83249-k.

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47

Ji, Baoyu, Liangshuang Xuan, Yunxiang Zhang, Wenrong Mu, Kee-Yoeup Paek, So-Young Park, Juan Wang, and Wenyuan Gao. "Application of Data Modeling, Instrument Engineering and Nanomaterials in Selected Medid the Scientific Recinal Plant Tissue Culture." Plants 12, no. 7 (March 30, 2023): 1505. http://dx.doi.org/10.3390/plants12071505.

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At present, most precious compounds are still obtained by plant cultivation such as ginsenosides, glycyrrhizic acid, and paclitaxel, which cannot be easily obtained by artificial synthesis. Plant tissue culture technology is the most commonly used biotechnology tool, which can be used for a variety of studies such as the production of natural compounds, functional gene research, plant micropropagation, plant breeding, and crop improvement. Tissue culture material is a basic and important part of this issue. The formation of different plant tissues and natural products is affected by growth conditions and endogenous substances. The accumulation of secondary metabolites are affected by plant tissue type, culture method, and environmental stress. Multi-domain technologies are developing rapidly, and they have made outstanding contributions to the application of plant tissue culture. The modes of action have their own characteristics, covering the whole process of plant tissue from the induction, culture, and production of natural secondary metabolites. This paper reviews the induction mechanism of different plant tissues and the application of multi-domain technologies such as artificial intelligence, biosensors, bioreactors, multi-omics monitoring, and nanomaterials in plant tissue culture and the production of secondary metabolites. This will help to improve the tissue culture technology of medicinal plants and increase the availability and the yield of natural metabolites.
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48

Sidorov, V. A. "PLANT TISSUE CULTURE IN BIOTECHNOLOGY: RECENT ADVANCES IN TRANSFORMATION THROUGH SOMATIC EMBRYOGENESIS." Biotechnologia Acta 6, no. 4 (2013): 118–31. http://dx.doi.org/10.15407/biotech6.04.118.

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49

Gaspar, Thomas, Claire Kevers, Claude Penel, Hubert Greppin, David M. Reid, and Trevor A. Thorpe. "Plant hormones and plant growth regulators in plant tissue culture." In Vitro Cellular & Developmental Biology - Plant 32, no. 4 (October 1996): 272–89. http://dx.doi.org/10.1007/bf02822700.

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50

Mahato, Asmita, Goma Chaudhari, Gaurave Banjade, and Shreesha Uprety. "TISSUE CULTURE AND ITS APPLICATION IN MODERN AGRICULTURE." i TECH MAG 5 (January 3, 2023): 09–11. http://dx.doi.org/10.26480/itechmag.05.2023.09.11.

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Tissue culture is a technique of growing plant cells, tissues and organs in an artificial prepared medium under aseptic conditions, which was invented by Gottlieb Haberlandt in 1902. Plant tissue culture is based on cellular totipotency, dedifferentiation, and redifferentiation. This paper overviews about procedures and applications of tissue culture by the analysis of numerous published studies. Compared to normal plant breeding methods, which take substantially longer, crops produced through tissue culture is developed through accurate and time-saving methods. It enables the recovery of embryos created by incompatible crosses, eliminates the phenomena of seed dormancy seen in plant species, and the life cycle of some species whose life cycles are relatively long are shorten. Even in situations where the species would typically have poor rates of multiplication, tissue culture enables the quick generation of a large number of plants. Also tissue culture required less space for multiplication of clones. An agriculturally valuable phenotype should come from the genetic diversity recovered from tissue culture regenerated plants. There are numerous issues arises in tissue culture. Researchers are having difficulty propagating plant tissues and acclimating in vitro produced plants in their natural habitat. However, greater understanding and study will lead to more widespread use of tissue culture.
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