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Dissertations / Theses on the topic 'Plant tissue culture'

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Published: 4 June 2021
Last updated: 30 July 2024

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1

Jana, M. M. "Studies on plant tissue culture." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1998. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3394.

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2

Morwal, G. C. "Biochemical studies in plant tissue culture." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1994. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2826.

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3

Muralidharan, E. M. "Biochemical studies in plant tissue culture." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1990. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2976.

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4

Sheibani, Ahmad. "Tissue culture studies of Pistacia." Thesis, University of Salford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.238801.

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5

Al-Ani, Nabeel K. "Some epigenetic effects in plant tissue culture." Thesis, Aberystwyth University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.659362.

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6

SINGLA, ANUPAM. "ENHANCED BIOACTIVE COMPOUNDS IN MEDICINAL PLANT USING PLANT TISSUE CULTURE." Thesis, DELHI TECHNOLOGICAL UNIVERSITY, 2021. http://dspace.dtu.ac.in:8080/jspui/handle/repository/18478.

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Bioactive chemicals are described as dietary components that affect people who consume those substances in their physiological as well as cellular function. Flavonoids, anthocyanins, tannins, berets, carotenoids, plant sterols and glucosinolates are all included. These may well be present most often in fruits and vegetables; offer anti - oxidant, anti-inflammatory but also antiviral activities; and therefore, can shield humans toward chronic disorders and metabolism. These favorable effects empower researchers to create novel functional foods containing prospective protective and healthful. Cardiovascular disease is the cardiac or blood vessel dysfunctional action. An inadequate heart and blood vessel function boosts the heart attack risk, heart failure, sudden death, stroke and heart rhythm disorders, resulting to diminished standard of health and a shorter life expectancy. Plant tissue culture is an effective venue for the generation of secondary metabolites along with its diverse implications. Diverse plant- based strategies including such callus or suspension cultivation are utilized generally in the synthesis of secondary plant metabolites. Several novel approaches which aim to have a rather significant and neglected influence on secondary metabolite synthesis.
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7

James, V. J. "Regulation of xenobiotic catabolism in plant tissue culture." Thesis, Cardiff University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380205.

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8

Wardrop, Julie. "Biotechnological applications of perfluorochemical liquids in plant tissue culture." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389475.

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9

GAUTAM, SHRUTI. "ENHANCED POLYPHENOL PRODUCTION IN MEDICINAL PLANTS USING TISSUE CULTURE." Thesis, DELHI TECHNOLOGICAL UNIVERSITY, 2021. http://dspace.dtu.ac.in:8080/jspui/handle/repository/18481.

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Medicinal plants are an indispensable source of phytochemicals. Polyphenols are one of the most prominent bioactive components produced by medicinal plants. Polyphenols are the plant’s secondary metabolites which are secreted in response to any kind of abiotic or biotic stress. The stress can be due to harmful UV rays, pathogen attack, infection, temperature variation, nutrient deficiency or any kind of infection. These polyphenols are responsible for providing medicinal properties to plants. The polyphenols are great therapeutic agents for humans and are widely used for manufacture of herbal drugs. The polyphenols have found to be strong antioxidant properties and protects epidermal layer from various damages. Polyphenols when either supplied in diet or applied topically have found to be protective effects on skin. It is known to have anti- inflammatory, anti-oxidant, anti- carcinogenesis, anti- melanogenesis property. For meeting such a high demand of polyphenols for therapeutic use, sustainable production of polyphenols is necessary as their extraction from natural sources leads to extinction of rare medicinal plant species. For this, the invitro plant tissue culture proves to be an efficient method of propagating rare medicinal plants and optimizing various environmental conditions for maximizing the production of polyphenols.
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10

Sinha, Debleena. "Development of an In Vitro Protoplast Culture System for Albizia Lebek (L.) Benth., an Economically Important Leguminous Tree." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc500422/.

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An in vitro system of generating protoplasts from their callus cultures was established. The friable callus was more productive in terms of producing protoplasts than the green compact callus. The concentration of the various cell wall degrading enzymes had an effect on the viability of the protoplasts in the medium. The protoplast system developed from the experiments was stable and could be used for the transformation experiments of Albizia lebek and for other plant improvement practices.
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11

Taeb, Abdulkarim Giumaa. "Influence of culture environment on tulip micropropagation." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328788.

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12

Khalid, Norzulaani. "Somaclonal variation through tissue culture studies in Chrysanthemum morifolium." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329847.

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13

Al, Kaabi Helel Humaid Saed Humaid. "Date palm tissue culture and AFLP analysis of plant variability." Thesis, Imperial College London, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409314.

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14

Ghadimzadeh, Mortaza. "Studies of protoplast and liposome techniques in plant tissue culture." Thesis, University of Salford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.255239.

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15

Tabe, R. H. "Biochemical studies in plant tissue culture with reference to hyperhydration." Thesis(M.Sc.), CSIR-National Chemical Laboratory, Pune, 2000. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2128.

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16

Lappin, G. J. "The biotransformation of monoterpenoids by plant cells in axenic culture." Thesis, University of Westminster, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382797.

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17

Watson, L. D. "Induction and assessment of plant cell membrane permeability." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233331.

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This thesis describes the isolation, immobilisation and permeabilisation of Digitalis lanata (foxglove) and Nicotiana tabacum (tobacco) plant cells and protoplasts. Protoplasts were isolated from leaves of Digitalis lanata and Nicotiana tabacum and cultured in conditions of varying osmotic potential, illumination and cell density in order to achieve maximum cell stability. The regeneration of cellulose cell walls in Nicotiana tabacum protoplasts could be inhibited by the addition of dilute concentrations of the herbicide 2,6-Dichlorobenzonitrile. At concentrations of 2-10 mg/l in culture medium cell wall regeneration could be prevented for up to 6-8 weeks. Isolated cells and protoplasts were stabilised by entrapment within a beaded agarose support matrix. Nicotiana tabacum protoplasts and cells, immobilised within an agarose beaded support matrix were used to develop a technique to permeabilise and destabilise the cell membranes. Cells or protoplasts pre-loaded with 6-Carboxy-Fluorescein, Neutral Red stains and 14C-Sucrose or 86Rb+ radioactive tracers were employed as markers for cell permeability or leakiness. Efflux or Compartmental analysis was used to determine the influence of various selected permeabilising agents on the integrity and the leakiness of either protoplast or cell membranes. Immobilised protoplasts or cells were subjected to a three - step procedure involving initial loading with 86Rb+ tracer, a membrane permeabilisation step and finally a recovery step. Protoplast membrane stability could not be regained after the recovery step, following a 30 minute period of permeabilisation with 50 mM acetic acid in culture medium. Immobilised cells could, however, regain membrane integrity with good intracellular retention of 86Rb+ tracer ion during the efflux experiment. Thus, immobilised Nicotiana tabacum cells could be made reversibly permeable with the use of specific agents. It is anticipated that such techniques which encourage reversible permeabilisation of immobilised cells have potential in larger scale plant cell culture systems to effect the release of intracellularly stored, useful secondary metabolites. There are also possibilities for the inclusion of exogenous precursors, cofactors and foreign genetic material which would increase compound yield and may produce novel secondary metabolites.
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18

Blakemore, Philip Alexander. "Optimisation of steam reconditioning for regrowth-ash and plantation-grown eucalypt species." Connect to full text, 2008. http://ses.library.usyd.edu.au/handle/2123/2343.

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Thesis (Ph. D.)--University of Sydney, 2008.
Includes graphs and tables. Includes list of publications: p. iv. Title from title screen (viewed May 5, 2008). Thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the School of Chemical and Biomolecular Engineering. Includes bibliographical references. Also available in print form.
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19

Gribble, Karleen Dawn. "Towards an understanding of the physiological abnormality of tissue cultured plants known as vitrification /." [Richmond, N.S.W.] : Horticulture, University of Western Sydney, Hawkesbury, 1999. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030513.144109/index.html.

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Thesis (Ph.D.) -- University of Western Sydney, Hawkesbury, 1999.
Thesis submitted for the degree of Doctor of Philosophy. Spine title: Towards an understanding of vitrification in tissue cultured plants. Includes bibliographical references (leaves 175-203).
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20

Redway, F. A. "Regeneration in tissue and protoplast culture of Sainpaulia ionantha (African violet)." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382673.

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21

Heyenga, Gerard. "Tissue culture of Podophyllum hexandrum and production of anticancer ligands." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235985.

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22

THOMAS, JOHN CALVIN. "THE CONSEQUENCES OF BROMODEOXYURIDINE TREATMENT IN PLANT TISSUE CULTURES (REGENERATION, REPLICATION)." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183989.

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Plant tissue culture regeneration is chiefly regulated by exogenous phytohormones. To stop regeneration and induce undifferentiated callus growth auxins are used. Unfortunately auxins influence many plant responses, most unrelated to development. Using the thymidine analogue 5-bromodeoxyuridine (BrdU) a phytohormone independent means for differentiation inhibition has been developed. Studies were focused on the target site and mechanism of BrdU action. The BrdU inhibited step in development is indicative of a plant response necessary for normal differentiation. BrdU (5-30 uM) interrupts callus growth in all tested plants. Exogenous cytokinin does not restore growth while thymidine and deoxycytidine rescue plant growth and differentiation in the presence of BrdU. Endogenous cytokinin levels are not greatly affected by subtoxic BrdU levels and indicate that cytokinin and BrdU act upon independent sites. Domestic carrot cells were used in further studies. Normally carrot cells are undifferentiated in medium with the auxin 2,4D. When 2,4D is removed, somatic embryogenesis takes place. By including 5 uM BrdU in the hormoneless medium, the cells fail to differentiate. The growth of carrots in 2,4D is not affected by 5 uM BrdU. Thus, BrdU influences growth during differentiation to a greater extent than the growth of callus cells. BrdU is effective in halting development when applied 0-24 hours after differentiation induction. An event required for differentiation (the first and second replications) must take place at this time. BrdU action begins with DNA incorporation. The consequent cellular replication becomes slowed and DNA repair results. At the same time RNA and protein levels are similar in BrdU treated and untreated cultures. BrdU thymidine substitution into DNA increases from 28% (2 days) to 68% (3 days) after embryogenic induction. A second BrdU effect follows DNA incorporation. Factors (MIFs) in the medium of BrdU treated cells arrest differentiation. After BrdU is repaired from the DNA, the cells are only able to differentiate after a medium change. Understanding MIF production could explain why some plants differentiate more readily than others. BrdU provides the means for further study of MIF's in the auxin-free inhibition of development.
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23

Holden, Peter Richard. "Variability in cultured cells of Capsicum Spp." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/10960.

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24

Ubaid, R. H. "Plant tissue culture and prostaglandin production from a range of Allium species." Thesis, University of Salford, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381734.

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25

Hamidoghli, Yousef. "Production and identification of interspecific potato somatic hybrids." Thesis, University of the West of Scotland, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283091.

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26

Spencer, Andrew. "The development of shooty teratomas in Mentha species by genetic manipulation and studies on their growth and terpene production in vitro." Thesis, University of East Anglia, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292258.

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27

Gibbs, Margaret Joan. "Genetic engineering of the forage legume Lotus corniculatus using Agrobacterium : mediated transformation systems." Thesis, Durham University, 1991. http://etheses.dur.ac.uk/6040/.

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Gene transfer vectors based on the Agrobacterium tumefaciens Ti plasmid were used to develop a successful disarmed Agrobacterium tumefaciens-mediated transformation method for Lotus comiculatus. A binary vector construct, pJIT73, was used during the development of the Agrobacterium tumefaciens transformation system due to its selectable (Aph IV, nos- neo) and scorable markers. The effects of the antibiotics geneticin (G-418) and hygromycin B were studied. Use of kill curves and selection delay experiments allowed potentially suitable selection pressure parameters to be proposed. Using such selection during transformation experiments led to further optimisation of this stage of transformation. The influence of plant hormones on the regeneration of Lotus comiculatus explants was investigated and a modification of an established protocol using leaf explants was introduced as an attempt to reduce the overall time of regeneration. Various explants were used but leaf pieces were chosen as the most suitable explant on which to focus research. So, through alteration of various stages, including length of cocultivation and subsequent decontamination within the transformation process, a successful method was developed. Experiments indicated the optimum Agrobacterium tumefaciens strain to be used with Lotus comiculatus was the disarmed Ach5 type, LBA4404(pAL4404). Transgenic Lotus comiculatus plants were produced which expressed the scorable marker β-Glucuronidase gene (GUS) and the selectable marker for hygromycin B resistance, AphIV. Gene transfer was confirmed by Southern blotting. The new Agrobacterium tumefaciens-mediated vector system was used to introduce the cowpea trypsin inhibitor gene (CpTi) into Lotus comiculatus. However, although there was evidence for transformed callus development, no shoots were induced. By the use of previously established Agrobacterium rhizogenes-mediated system, an attempt was made to introduce the pea lectin gene (psl) into Lotus corniculatus. Hairy root regenerants were produced but genetic transfer was unconfirmed and attempted investigation of the plant - Rhizobium symbiosis involving Lotus corniculatus was not fulfilled.
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28

Khatun, Asma. "The genetic manipulation of jute (Corchorus) species." Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335652.

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29

Smith, Elaine Francis. "The preparation of micropropagated plantlets for transplantation." Thesis, University of East London, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258546.

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30

Hudson, G. "Micropropagation and low temperature storage of Dieffenbachia." Thesis, University of East London, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370763.

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31

Kirby, Nigel John. "An investigation of metabolite release from plant cells in vitro to their surrounding medium." Thesis, University of the West of England, Bristol, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329861.

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32

Satchwell, Christa Elizabeth. "Investigation of the nutrient requirements of Pinus caribaea Morelet in vitro." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305742.

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33

Shih, Sharon Min-Hsuan Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Transient viral infection of plant tissue culture and plants for production of virus and foreign protein." Awarded by:University of New South Wales, 2007. http://handle.unsw.edu.au/1959.4/34967.

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This work was aimed to investigate the basic viral infection protocols mainly focusing on Nicotiana benthamiana hairy root cultures and wild-type tobacco mosaic virus (TMV). The application of transgenic virus containing the gene for green fluorescent protein (GFP) for foreign protein production in plant tissue cultures and whole plants was also studied. The effect on viral accumulation of the form of plant tissue culture used, such as hairy roots, shooty teratomas and suspended cells, was investigated. Viral infection was shown to have no effect on culture growth and morphology. Hairy root cultures are a superior host for viral propagation and production in vitro. The maximum specific rate of viral accumulation occurred mainly during the root growth phase. The average maximum virus concentration in the hairy roots was 0.82 ?? 0.14 mg g-1 dry weight and virus protein represented a maximum of approximately 6% of total soluble protein in the root biomass. Proportional scale-up of TMVinfected hairy roots in shake flasks and bioreactors can be achieved without changing the average virus concentration accumulated in the hairy roots. The level of viral accumulation was much lower in N. benthamiana hairy roots infected with transgenic virus containing GFP (TMVGFPC3) compared with TMV and low levels or no GFP was detected. Viral accumulation and GFP production in whole plants was studied using different generations of transgenic TMV-GFPC3 virus. Hybrid viruses with the foreign gene GFPC3 deleted may have been formed in successive TMV-GFPC3 generations, resulting in the loss of GFP production and enhanced viral infectivity. In vitro generated RNA transcript and first generation TMV-GFPC3 were found to be more suitable for infection than the second generation TMV-GFPC3. However, the accumulation of GFP and virus concentration did not occur at the same ratio. Provided a more genetically stable transgenic viral vector is used for infection, transient viral infection of hairy roots can be a potential alternative system for foreign protein production than plants grown in the field as the containment or safety issues can be addressed.
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34

Kaparakis, Georgios. "In vitro culture of pepper (Capsicum annuum L.)." Thesis, University of Nottingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297989.

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35

Gunasekare, M. T. K. "In vitro culture directed towards plant improvement of tea (Camellia sinensis var. assamica)." Thesis, University of Southampton, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241937.

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36

Qouta, Lolita Abdulla. "The biochemistry and molecular biology of intercellular adhesion in plant tissue culture." Thesis, University of Glasgow, 2008. http://theses.gla.ac.uk/302/.

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The adhesion between neighbouring plant cells is established as cells are formed during cytokinesis through the middle lamella that is made principally of pectins and proteins. Pectins are secreted into the cell wall in a highly methylesterified form and subsequently de-esterified in muro by pectin methyl esterase (PME, E.C. 3.1.11). The present study reports on the biochemical characterization and immunochemical analyses of phosphate buffer/EDTA pectic extracts associated with cell-cell adhesion in suspension cultures of wild type (WT), salt tolerant (HHS) cell lines and synchronized Arabidopsis suspension cultures. Using the synchronized cultures, The PME-mediated configuration of pectins at the onset of adhesion during cytokinesis, was assessed through the analysis of the expression patterns of the PME isoforms annotated to be expressed throughout the cell cycle The wild type Arabidopsis seemed to maintain the intercellular adhesion through the gelling of the highly methylated JIM7 recognized homogalacturonans that were shown to be abundant in the primary cell walls, middle lamellae and cellular junctions, possibly due to the hydrophobic interactions between the methoxy groups. The rhamnogalacturonan-I fraction was rich in arabinan side chains reflecting the proliferative state of the cells. The increase in arabinan content was accompanied by a reduction in the galactan content 4 days after subculturing. The cell walls of salt tolerant Arabidopsis contained the JIM7 and LM7recognized epitopes along with a high degree of branching of rhamnogalacturonan-I carrying galactans and arabinans as side chains. The change in the detected epitopes is thought to play a role in the ability of the cells to withstand the high osmotic pressure and increase the in the level of adhesion between cells. The JIM5 low methylesterified HGs were less abundant in both cultures, and the absence of the 2F4 antibody recognizing the Ca2+ egg boxes could be attributed to the scarce amounts of Ca2+ present in the culturing medium The immunochemical studies of the pectin extracted from the synchronized Arabidopsis suspension cultures after washing out aphidicolin indicated that the recognition of both of JIM7 and JIM5 varied in parallel during the cell cycle, whereas, the recognition of arabinan increased during the cell division. The sequence and phylogenetic analysis of ten PME isoforms that were annotated to be expressed at one or more phases of the cell cycle of synchronized Arabidopsis thaliana suspension cultures (Menges and Murray, 2002 and 2003), revealed that only five of these genes could be PMEs. The genes At4g02330, At1g02810, At2g26440, and At2g47550 were thought to be of type II PMEs which have a pre-pro-catalytic domains and At5g47500 is a type I PME that lack the pro-region. The amino acid sequence of At4g12390 showed similarities with the N-terminal pro-peptides of plant PME and invertase inhibitors. The expression of several PME genes was studied in suspension cultures of Arabidopsis thaliana synchronised using aphidicolin. Semi-quantitative PCR experiments showed that the expression of At5g47500 transcript was always detected during M phase of the cell cycle. The rest of the genes failed to show consistent patterns of expression. Northern blots revealed that mRNA coding for At5g47500 decreases during S and G2 phases and accumulates during the M phase of the cell cycle. Our results suggest that this PME isoform is involved in the modulations of the cell walls as the cells are going through division and cytokinesis.
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37

Hussain, Shayne. "Evaluation of microbial extracts for contamination control in plant tissue culture systems." Thesis, University of Plymouth, 1991. http://hdl.handle.net/10026.1/2716.

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Culture filtrates of 13 microbial antagonists exhibited in vitro growth inhibition of a range of test contaminations of herbaceous and woody plant tissue culture systems. Filtrates produced by Bacillus subtilis and Trichoderma viride isolates displayed the greatest broad- range inhibitory activity. Microscopic analysis of antagonized fungal mycelia revealed altered hyphal morphology. Maximum filtrate inhibitory activity was produced when selected antagonists were cultured within a pH range of 5-7 and a temperature range of 20-35°C. Filtrates were thermo-stable at 70°C and could be stored for up to 4 weeks with only a minimal reduction in their inhibitory activity. Bulk-volume production of inhibitory filtrates of a T. viride isolate was achieved by optimization of fermentation pH, temperature, and aeration conditions. Plant culture species displayed different responses when grown on media incorporated with microbial filtrates. Microscopy studies at the cellular level revealed reduced cell densities and cellular distortion in plant tissues treated with phytotoxic doses of microbial filtrates. Non-phytotoxic doses of filtrated produced by the B. subtilis and T. viride isolates produced a reduction in the density of opportunistic contaminations in herbaceous plant tissue cultures when applied as prophylactic treatments. Microbial filtrates proved totally ineffective when employed as post-infection sterilants in contaminated plant cultures. The efficacy of selected microbial filtrates was not comparable to that of conventional antibiotics when assessed for their ability to control contamination levels in herbaceous and woody plant culture systems. Further purification of microbial filtrates for enhanced inhibitory activity is discussed along with the possibility of co-cultivation of micro-organisms with plant tissue cultures as a means of biocontrol of phytopathogens.
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38

Mao, Ashiho A. "Tissue culture of a range of plant species from north-east India." Thesis, University of Reading, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308055.

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39

Compton, Michael E. "De novo morphogenesis on tomato thin cell layers and variation for genetic recombination among plantlets regenerated from tissue culture." Diss., This resource online, 1990. http://scholar.lib.vt.edu/theses/available/etd-09162005-115005/.

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40

Patil, Rajashekar M. "Tissue culture and transformation of rice (oryza sativa L.) using tobacco nurse cells /." Title page, contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09APSM/09apsmp298.pdf.

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41

Wilson, Zoe Amanda. "A study of the tissue culture and genetic manipulation of rubber (Hevea brasiliensis)." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235687.

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42

Zalat, Eman S. "Plant tissue and cell culture of taxus species as a source of taxanes." Thesis, University of Manchester, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.488110.

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43

Borrino, E. M. "Plant tissue culture : an analysis of variation of in-vitro response to salinity." Thesis, University of Reading, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316040.

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44

Hendre, R. R. "Molecular basis of differentiation in plant tissue culture with special reference to sugarcane." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1988. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3309.

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45

Obae, Samuel G. "Genetic characterization, ginsenoside analysis and micropropagation of American ginseng (Panax quinquefolius L.)." Morgantown, W. Va. : [West Virginia University Libraries], 2010. http://hdl.handle.net/10450/11231.

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Thesis (Ph. D.)--West Virginia University, 2010.
Title from document title page. Document formatted into pages; contains ix, 160 p. : ill. (some col.), col. maps. Includes abstract. Includes bibliographical references.
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46

Tsoktouridis, Georgios. "Molecular detection and characterisation of bacteria intimately associated with Billbergia magnifica ssp. acutisepalia during micropropagation by axillary shoot proliferation and somatic embryogenesis." Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368912.

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47

Saka, Kamel. "REGENERATION OF COTTON (GOSSYPIUM HIRSUTUM L.) CALLUS PROTOPLASTS TO MACROCALLI." Thesis, The University of Arizona, 1985. http://hdl.handle.net/10150/275376.

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48

Mlungwana, Asanda. "In-vitro propagation studies of the endangered succulents Drosanthemum Micans and Drosanthemum Hallii (Aizoaceae)." Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2748.

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Thesis (MTech (Horticulture))--Cape Peninsula University of Technology, 2018.
Drosanthemum micans and Drosanthemum hallii are endangered succulent shrubs of horticultural and medicinal value. They are restricted to the Succulent Karroo, which is one of the world’s biodiversity hotspots. The species risk extinction from illegal over-harvesting for water-wise gardens, erosion by occasional flush floods from ephemeral rivers, competition from alien invasive species, overgrazing and clearing of land for agriculture and human settlement. Although seeds and cuttings may be used in propagating these species, they often require seasonal collection and planting and cuttings struggle to establish, hence the need for in-vitro propagation as an alternative solution. Thus, the main objective of the study was to develop a method for rapid in-vitro shoot and root multiplication and acclimatization of D. micans and D. hallii. To initiate shoot formation, disinfected leaf and stem nodal explants were cultured in Murashige and Skoog (1962) media supplemented with different rates (0, 10, 20 or 30μM) of 2-isopentyladenine, 6-Benzyladenine and kinetin for D. hallii or 2-isopentyladenine, 6-Benzyladenine and Thiadiazuron for D. micans. Shoots from explants were rooted in varying rates (0, 10, 20 or 30μM) of IAA for root initiation. Three media, which were used in previous studies, were tested for acclimatization of rooted explants in i) vermiculite, ii) sand (50%): vermiculite (50%) or iii) sand (75%): perlite (25%). For quantitative evaluation of plant stress, chlorophyll fluorescence index (Fv/Fm) was measured as a proxy for plant stressf stress. It emerged that stem nodal explants of D. hallii tend to produce multiple shoots whilst leaf explants tended to produce callus when cultured in full-strength Murashige and Skoog (1962). Shoot multiplication was optimal in both D. hallii and D. micans at 10 μM of kinetin. Root formation in both D. hallii and D. micans only occurred when shoots were transferred to a full-strength Murashige and Skoog (1962) media without any phytohormones added. The intensity of tissue browning increased at higher levels of cytokinins, suggesting an interaction of plant growth regulators with exudates from explants. Different acclimatization media tested showed no significant differences in the level of stress (Fv/Fm). It is recommended that Murashige and Skoog (1962) media with10 μM kinetin be used for shoot development and multiplication, followed by transfer of the shoots to fresh full-strength Murashige and Skoog (1962) media without hormones for root development. Acclimatization of the rooted explants was possible in one of the following media: i) vermiculite, ii) sand (50%): vermiculite (50%) or iii) sand (75%): perlite (25%) and in a misted greenhouse (ca. 60% RH), with gradual weekly reductions in humidity by 10% over 2 weeks.
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49

Lormand, Katherine Bradbury 1961. "RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS." Thesis, The University of Arizona, 1987. http://hdl.handle.net/10150/276474.

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The responses of the tepary bean (Phaseolus acutifolius) to in vitro tissue culture systems were documented. Tests were conducted to identify the optimal auxin and cytokinin combinations required for optimal callus growth. Regeneration experiments were conducted to: (1) determine the effect of explant source and age on regeneration, (2) effect of callus age on regeneration, (3) the cultivation status of the explant source, and (4) the effects of nutritional additives on somatic embryogenesis. The callus was easily induced and maintained in all hormonal medias except those containing IAA and 6BA. The results for regeneration were most promising from cultures derived from immature cotyledon tissue. Ammonium Chloride, glutamine and Absisic acid appeared to have little affect on embryogenesis, however the addition of kinetin enabled the embryos to develop to the torpedo stage. Callus age and cultivative status of explant source had no effects on plantlet regeneration.
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50

Tan, Hooi Sin. "Proteomics analysis of somatic embryogenesis in tissue culture of oil palm (Elaeis guineensis Jacq)." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/33755/.

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Oil palm is an important commercial crop in Malaysia where Malaysia is the second largest producer and exporter of palm oilin the world. In order to meet the increasing demand for palm oil, elite oil palm planting materials with higher palm oil yield are the desirable planting materials. Hence, the oil palm plantation companies have incorporated in vitro micropropagation technique through somatic embryogenesis in producing elite oil palm. However, low embryogenesis rate has hampered large production of elite oil palm ramets. In this study, proteomic technology was deployed to compare protein expression and identify differential expressed protein between high and low proliferated embryogenic lines of oil palm tissue culture. From the study, total protein of oil palm young and old leaves was extracted using an optimized trichloroacetic acid/acetone precipitation protocol followed by polyethylene glycol (PEG) fractionation to isolate low abundance proteins. Then, the extracted proteins were separated on two-dimensional (2D) gel electrophoresis and protein profiles between the high and low proliferated embryogenic lines were compared. Total of 40 differentially expressed protein spots were isolated from the 2D gel for mass spectrophotometry (MS/MS) identification. However, only 26 out of 40 protein spots were identified and just 8 of the identified protein spots were isolated from young leaves. Quantitative real-time PCR were conducted on 17 proteins candidates to study on the relationship between the protein and mRNA expression level. There was 29% of the 17 proteins’ expression showed linear correlation with their mRNA expression. These proteins candidates were highlighted for further validation in the future.
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