Journal articles: 'Plant micropropagation'

1

Giles, Kenneth L., and Walter M. Morgan. "Industrial-scale plant micropropagation." Trends in Biotechnology 5, no. 2 (February 1987): 35–39. http://dx.doi.org/10.1016/0167-7799(87)90035-7.

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2

RANCILLAC, M. J., and J. G. NOURRISSEAU. "MICROPROPAGATION AND STRAWBERRY PLANT QUALITY." Acta Horticulturae, no. 265 (December 1989): 343–48. http://dx.doi.org/10.17660/actahortic.1989.265.50.

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3

Clemente Muñoz, Margarita. "Micropropagation of endangered plant species." Ecologia mediterranea 21, no. 1 (1995): 291–97. http://dx.doi.org/10.3406/ecmed.1995.1779.

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4

R. E. Young, A. Hale, N. D. Camper, R. J. Keese, and J. W. Adelberg. "APPROACHING MECHANIZATION OF PLANT MICROPROPAGATION." Transactions of the ASAE 34, no. 1 (1991): 0328. http://dx.doi.org/10.13031/2013.31666.

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Kulchin, Yuriy Nikolaevich, Olga Valerievna Nakonechnaya, Irina Victorovna Gafitskaya, Olga Vadimovna Grishchenko, Tatyana Yuryevna Epifanova, Irina Yuryevna Orlovskaya, Yuriy Nikolaevich Zhuravlev, and Evgenii Petrovich Subbotin. "Plant Morphogenesis under Different Light Intensity." Defect and Diffusion Forum 386 (September 2018): 201–6. http://dx.doi.org/10.4028/www.scientific.net/ddf.386.201.

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The innovative LED light source (Sun Box) with irradiation spectrum close to the sun spectrum in the wavelength range 440-660 nm was used in experiment for study the influence of light intensity (75, 135, 230 and 382 μmol/s*m2) on the growth and development of plants. Standard fluorescent lighting was used as a control. The experiments were carried out on plantlets ofStevia rebaudianaandSolanum tuberosum, cvs. Snegir, Rozhdestvenskiy and Kamchatskii)in vitro. The illumination intensity of 75 and 230 μmol/s*m2promoted development ofS. rebaudianaplantlets with optimal values of morphometric parameters and well developed roots, which is important for plantlet adaptation to soil conditions. ForS. tuberosumplantlets (Snegir and Rozhdestvenskiy cultivars), radiation intensity of 135 μmol/s*m2was optimal for micropropagation. The illumination intensity of 230 μmol/s*m2led to a formation of plantlets with the largest total fresh mass among experimental groups. Sun Box light with intensity of 75 μmol/s*m2could be applicated for micropropagation of these cultivars: plantlets were the highest with the largest internodes number. Thus, the plant response to different light intensity was species-spesific, and – in case of potato plantlets – cultivar-spesific. The use of artificial light sources with distinct PPFD level could be preferable forS. tuberosumandS. rebaudianaplantlet micropropagationin vitro, as it could shorten the cultivation time, accelerate cultivation time, and reduce the cost of electricity.

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Singh, M., S. Sonkusale, Ch Niratker, and P. Shukla. "Micropropagation of Shorea robusta: an economically important woody plant." Journal of Forest Science 60, No. 2 (March 4, 2014): 70–74. http://dx.doi.org/10.17221/80/2013-jfs.

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Shorea robusta is a valuable tree species which provides good quality timber along with other useful materials like seeds which can be used as a source of starch. Woody plants are difficult to regenerate under in vitro conditions and only some success has been achieved so far. Here we have presented the data for successful in vitro regeneration of S. robusta using nodal explants. Shoot proliferation and rooting were also successfully achieved in subsequent subcultures. The best medium for shoot initiation and proliferation was found to be WPM with 1.0 mg·l–1 BAP and 0.5 mg·l–1 NAA and 1.0 mg·l–1 BAP +0.5 mg·l–1 NAA, respectively. Likewise for rooting WPM medium with 0.5 mg·l–1 IBA was found to be the best medium.

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7

Kozai, Toyoki. "Photoautotrophic micropropagation." In Vitro Cellular & Developmental Biology - Plant 27, no. 2 (April 1991): 47–51. http://dx.doi.org/10.1007/bf02632127.

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8

Onay, Ahmet, Hakan Yildirim, Yelda Ozden Tokatli, Hulya Akdemir, and Veysel Suzerer. "Plant tissue culture techniques—Tools in plant micropropagation." Current Opinion in Biotechnology 22 (September 2011): S130. http://dx.doi.org/10.1016/j.copbio.2011.05.426.

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Gargiulo, Jennifer A., and Michael E. Kane. "941 AQUARIUM PLANT MICROPROPAGATION: CRYPTOCORYNE BECKETII." HortScience 29, no. 5 (May 1994): 568e—568. http://dx.doi.org/10.21273/hortsci.29.5.568e.

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The genus Cryptocoryne (Araceae) contains some of the most commercially important amphibious species used in the aquarium plant trade. However, seed production is rare and vegetative propagation by rhizome division is extremely slow. Procedures for in vitro establishment, axillary shoot proliferation and plantlet acclimatization of Cryptocoryne Becketti Thwaites ex Trimen were determined. Surface sterilized rhizomatous shoot tips were established on a medium consisting of Linsmaier & Skoog mineral salts and organics supplemented with 87.6 mM sucrose, 2.2 μM benzyladenine (BA) and 0.57 μM indole-3-acetic acid (IAA) solidified with 0.8% TC® Agar. Effects of medium supplementation with factorial combinations of BA (0 - 25 μM) and IAA (0 - 10 μM) on axillary shoot proliferation from single node explants were determined after 28 days. Maximum axillary shoot proliferation (`l-fold increase) occurred on medium supplemented with 25 μM BA and 1.0 μM IAA. Excellent microcutting rooting (100%) was achieved by direct sticking in Vergro Klay Mix A. Greenhouse acclimatization of rooted microcuttings was 100%.

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El-Banna, H. "Micropropagation of thyme plant (Thymus vulgaris)." Journal of Plant Production 8, no. 11 (November 1, 2017): 1221–27. http://dx.doi.org/10.21608/jpp.2017.41294.

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El-Banna, H. "Indirect Micropropagation of Thymus vulgaris Plant." Journal of Plant Production 8, no. 11 (November 1, 2017): 1241–46. http://dx.doi.org/10.21608/jpp.2017.41300.

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12

Chen, Chiachung. "Fluorescent Lighting Distribution for Plant Micropropagation." Biosystems Engineering 90, no. 3 (March 2005): 295–306. http://dx.doi.org/10.1016/j.biosystemseng.2004.10.005.

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13

Purshottam, Dhermendra Kumar, Pratibha Misra, and Ramesh Kumar Srivastava. "A Mutation Study on Gerbera jamesonii: An Important Ornamental Plant." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 6, no. 01 (January 31, 2020): 91–93. http://dx.doi.org/10.18811/ijpen.v6i01.10.

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The demand for variations in ornamental plants is always on high for the development of different types of color and other morphological changes etc. In this context, the mutation is a very much helpful and promising approach among the floriculturists and very well recognized for the development of novel varieties. Most of the researchers used the micropropagation techniques for large scale propagation of ornamental plants. Micropropagation not only enhances the rate of propagation but also produce true to type plants in a relatively short time and space. In this study, we use a combination of mutation and micropropagation strategies in Gerbera jamesonii plant.

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Keer, Kheiry, Elmundr Abughnia, Salem Hammud, Ahmed shaaban, Mohamed Abusanina, and Arij shaheen. "Micropropagation of Zingiber officinal roscoe." Journal of Misurata University for Agricultural Sciences, no. 01 (October 6, 2019): 38–50. http://dx.doi.org/10.36602/jmuas.2019.v01.01.04.

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This study was conducted in plant tissue culture In Biotechnology which belong to biotechnology research center (Tripoli – for Micropropagation of in order to study the response of Zingiber plant to In vitro micro propagation through plant tissue culture technology , while the study was beginning by Samples were collected , the samples from the local market and directly were put in dark For sprouting in order to obtain plant tissue which will be used for plant micro propagation. Sprouted buds growth were obtained the plant tissue were sterilized by use 2.5% Clorox and 70% ethanol in hood cabinet with sterilized conditions , then sterilized plant tissue were cultured in small gars contain Murashige and Skoog MS medim as control treatment and MS media supplemented with different concentrations of BA and NAA plant growth regulators while the treatments were ( 2 , 4mg/l BA ) and ( 2 mg/l BA + 0.5mg/l NAA) . Results of this study showed present a good response of Zingiber plant to micro propagation by tissue culture technology in all the treatments event control treatment moreover the results showed that the treatment of 2 mg/l BA gave the highest average of obtained number of brunches and root system growth , finally the obtained plants from the experiments were moved to adaptation stage by placed the plants in small puts contain peat moss fertilizer

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Abiri, Rambod, Narges Atabaki, Hazandy Abdul-Hamid, Ruzana Sanusi, Nor Aini Ab Shukor, Noor Azmi Shaharuddin, Siti Aqlima Ahmad, and Sonia Malik. "The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp." Forests 11, no. 11 (November 18, 2020): 1211. http://dx.doi.org/10.3390/f11111211.

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Micropropagation is a reliable technique in biotechnology and genetic engineering domain, which has been widely applied for rapid mass propagation of plants in vitro condition. Through micropropagation techniques, reproduction of plants can be attained from different explants using organogenesis and somatic embryogenesis. Over the decades, micropropagation techniques have offered tremendous potential for forest tree improvement. Eucalyptus is a woody plant species recalcitrant to in vitro culture. In general, the micropropagation of Eucalyptus culture processes and the genotype, environment surroundings, and age of explants in culture media is frequently linked with the occurrence of micropropagation variation. In the current review paper, an update of the most important physiological and molecular phenomena aspects of Eucalyptus micropropagation was linked to the most profound information. To achieve the mentioned target, the effect of plant growth regulators (PGRs), nutrients, other adjuvant and environmental features, as well as genetic interaction with morpho- and physiological mechanisms was studied from the induction to plant acclimatisation. On the other hand, important mechanisms behind the organogenesis and somatic embryogenesis of Eucalyptus are discussed. The information of current review paper will help researchers in choosing the optimum condition based on the scenario behind the tissue culture technique of Eucalyptus. However, more studies are required to identify and overcome some of the crucial bottlenecks in this economically important forest species to establish efficient micropropagation protocol at the industrial level.

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Huang, Feng H., Jameel M. Al-Khayri, and Edward E. Gbur. "Micropropagation ofAcacia mearnsii." In Vitro Cellular & Developmental Biology - Plant 30, no. 1 (January 1994): 70–74. http://dx.doi.org/10.1007/bf02632123.

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17

Pandya, Dhruv, Archana Mankad, and Himanshu Pandya. "COST EFFECTIVE MICROPROPAGATION OF POLYSCIASFRUTICOSA (L.) HARM." International Association of Biologicals and Computational Digest 1, no. 1 (May 2, 2022): 58–62. http://dx.doi.org/10.56588/iabcd.v1i1.16.

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Polysciasfruiticosa(L.) Harm is plant which belonging to Araliaceae family, also known as Ming Aralia. Micropropagation is a technique to develop hole plant from the part of the plant in In-vitro or controlled environmental conditions. The research work focused on the micropropagation of Polysciasfruticosa(L.) Harm. from the shoot apex of the plant. Here only the cytokinin was used for the production of whole plant and M. S. Media. Generally, in Micropropagation auxin is used for the rooting phase but in this research work only kinetin which was used for the better growth for rooting also. Mostly the plant producing rare flowers and propagated through cuttings. Here M. S. Media with one Kinetin hormone standardized for the successful production of the plant lets of Polysciasfruticosa (L.) Harm. with this technique within 60days we can produce no. of plantlets of Polysciasfruticosa (L.) Harm.

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Birmeta, Genet, Tura Safawo, Mulatu Geleta Dida, and Endashaw Bekele. "Critical Review on Plant Micropropagation of Ethiopian Plants Reported So Far: Existing Gaps, Required Standardization, and Future Research Direction." Advances in Agriculture 2022 (October 5, 2022): 1–22. http://dx.doi.org/10.1155/2022/5874899.

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Plant micropropagation research in Ethiopia requires concerted efforts to meet desired levels of application for sustainable utilization of the country's diverse plant genetic resources. The purpose of the present review is to provide an update on the results of plant micropropagation conducted so far in Ethiopia. It assessed their strengths and identified gaps in order to standardize research methods and indicate future research directions. Two cereals, three oil crops, three spices, five medicinal plants, two high-value crops, six fruit plants, nine root crops, and one endangered multipurpose shrub were reviewed. The assessment of previously published research was carried out in terms of methods used in the selection of ex plants and their disinfestations, culture vessels, and media used with a variety of combinations and concentrations of plant growth regulators, macro- and micronutrient requirements, culture environments, and genetic stability of regenerated plantlets. Further assessments include the utilization of plant growth-promoting microbes and applications of “omics” research in order to establish standardized, efficient, and cost-effective micropropagation techniques. The findings of the assessments are summarized and current advances are highlighted, along with recommendations for future plant micropropagation studies in the country.

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Bozkurt, Taner, Sezen Inan, Ijlal Dundar, and Selda Kozak. "Effect of Different Plant Growth Regulators on Micropropagation of Some Pitaya Varieties." Journal of Tropical Life Science 12, no. 2 (May 17, 2022): 183–90. http://dx.doi.org/10.11594/jtls.12.02.04.

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Pitaya belongs to the family Cactaceae and the genus Hylocereus. It is essential todevelop tissue culture protocols according to the appropriate variety to spread pitayacommercial production and ensure healthy sapling production. This study aimed todetermine the best plant growth regulators (PGRs) in the micropropagation process,and their effects on different pitaya cultivars were evaluated. Shoots of different pitayacultivars were cultured in Murashige and Skoog (MS) basal medium supplementedwith Indole-3-butyric (IBA), 6-benzyl amino purine (BAP), and gibberellic acid(GA3). The highest micropropagation coefficient was determined in Physical Graffitivariety cultured in MS medium supplemented with 2 mg/L BAP. The general evaluation based on the variety determined that the best micropropagation was in the RoyalRed variety. In in vitro rooting studies, the best rooting variety was Royal Red(54.47%), followed by Siam Red (50.33%), Physical Graffiti (47.75%), and SeoulKitchen (44.82%). It was determined that the Royal Red variety is grown in R2 (MSmedium supplemented with 1 mg/l IBA) medium gave the best results in all criteriaof shoot length (4.28 cm), root length (6.45 cm), and root formation on the face(74.48%). It was envisioned that these differences between the micropropagation,growth, and rooting of the cultivars used in the studies resulted from the cultivar characteristics.Keywords: Pitaya, In vitro, Micropropagation, Royal Red, Seoul Kitchen, Siam Red,Physical Graffiti

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Chen, Jianjun, and Richard J. Henny. "Commercial Production of Ornamental Tropical Foliage Plants: Micropropagation." EDIS 2015, no. 5 (August 5, 2015): 4. http://dx.doi.org/10.32473/edis-ep520-2015.

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Florida nursery operators need to understand plant propagation principles and techniques so they can grow enough plants for sale. Micropropagation is a way to culture plant tissue to rapidly propagate a large number of plants. This 4-page fact sheet presents an overview of micropropagation to help growers evaluate it as a propagation technique for their own nursery operations. Written by J. Chen and R. J. Henny, and published by the UF Department of Environmental Horticulture, May 2015. (Photo: J. Chen, UF/IFAS) ENH1259/EP520: Commercial Production of Ornamental Tropical Foliage Plants: Micropropagation (ufl.edu)

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Keret, R., M. Nakhooda, and P. N. Hills. "Optimisation of Eucalyptus micropropagation." South African Journal of Botany 115 (March 2018): 320. http://dx.doi.org/10.1016/j.sajb.2018.02.160.

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van Rensburg, Josephina G. J., Bela M. Vcelar, and Penelope A. Landby. "Micropropagation of Ornithogalum maculatum." South African Journal of Botany 55, no. 1 (February 1989): 137–39. http://dx.doi.org/10.1016/s0254-6299(16)31242-x.

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Swart, P. A., J. F. Finnie, and J. Van Staden. "Micropropagation of Romulea species." South African Journal of Botany 75, no. 2 (April 2009): 422. http://dx.doi.org/10.1016/j.sajb.2009.02.110.

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Ngunge, V. N. P., J. F. Finnie, and J. Van Staden. "Micropropagation of Tulbaghia species." South African Journal of Botany 76, no. 2 (April 2010): 400. http://dx.doi.org/10.1016/j.sajb.2010.02.035.

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THIRUPATHI, M, THIRUPATHI, M., and JAGANMOHAN REDDY, K. JAGANMOHAN REDDY, K. "Micropropagation Studies of Senna Alata (L.) Roxb. an Anti Allergenic Plant." Indian Journal of Applied Research 3, no. 12 (October 1, 2011): 65–68. http://dx.doi.org/10.15373/2249555x/dec2013/17.

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THIRUPATHI, M, THIRUPATHI, M., and JAGANMOHAN REDDY, K. JAGANMOHAN REDDY, K. "Micropropagation Studies of Senna Alata (L.) Roxb. an Anti Allergenic Plant." Indian Journal of Applied Research 4, no. 1 (October 1, 2011): 65–68. http://dx.doi.org/10.15373/2249555x/jan2014/21.

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27

T., Binish. "Micropropagation of traditional medicinal plant Ceropegia juncea." Annals of Plant Sciences 7, no. 2 (January 31, 2018): 1992. http://dx.doi.org/10.21746/aps.2018.7.2.2.

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Ceropegia species which possess wide medicinal properties are being used in different traditional medicinal systems that are used by tribal people for curing different ailments. Ceropegia juncea was reported to be the source of ‘Soma’, a plant drug of the Ayurvedic system of medicine. The plant extract is used for the treatment of anti-inflammatory, analgesic, antiulcer activities, liver disorders, hypotension, ulcerative condition and fever. It is also used as typical anesthetic agent. The present study was conducted to establish a protocol for in- vitro propagation of an endemic medicinal plant Ceropegia juncea maximum shoot proliferation better shoots with a sprouting frequency of 86% and with an average of 8.28 ±1.11 shoots /explants and attained a length of 5.37±0.74 cm was achieved on Murashige and Skoog’s, 1962 (MS) medium supplemented with 6-benzylaminopurine (BAP) 1.5 mg/L + NAA 1.0mg/L and highest rooting of in vitro derived shoots was achieved on half MS with IBA 0.75mg/L.

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Kadirova, Zukhra, Tashmukhamedova Shokhista, Dalimova Dilbar, Madjidova Rano, and Shovkatova Gulchehra. "Micropropagation of the medicinal plant Physalis alkekengi." National Journal of Physiology, Pharmacy and Pharmacology 9, no. 8 (2019): 1. http://dx.doi.org/10.5455/njppp.2019.9.0416329062019.

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Kane, Michael E., Edward F. Gilman, Matthew A. Jenks, and Thomas J. Sheehan. "Micropropagation of the Aquatic Plant Cryptocoryne lucens." HortScience 25, no. 6 (June 1990): 687–89. http://dx.doi.org/10.21273/hortsci.25.6.687.

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Procedures for in vitro establishment, rapid shoot proliferation, and ex vitro plantlet acclimatization of Cryptocoryne lucens de Witt were determined. Shoot cultures were established from surface-sterilized shoot tips cultured on Linsmaier and Skoog salts and vitamins medium (LS) solidified with 0.8% (w/v) agar and supplemented with 2.0 μm BA and 0.5 μm NAA. The effect of BA (0 to 20 μm) and 0.5 μm NAA on shoot multiplication from single-node and clustered triple-node shoot explants was determined after 35 days. The most efficient shoot proliferation (7.7 shoots/explant) occurred from single-node shoot explants cultured on LS + 20 μm BA and 0.5 μm NAA. Maximum plantlet establishment was achieved by direct sticking of triple-node (cluster) microcuttings in either soilless planting medium or polyurethane foam cubes. Production of highly branched salable plants from microcuttings was possible within 18 weeks. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA); 1-naphthaleneacetic acid (NAA).

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El-Liel, Eman F. A. bu, Abeer A. Mahmoud, Azza M. Salama, El-Mewafy A. E. El-Ghadban, and Mohamed K. Khalil. "Improved Micropropagation of Stevia (Stevia rebaudianaBertoni) Plant." Journal of Agricultural Studies 7, no. 1 (January 25, 2019): 1. http://dx.doi.org/10.5296/jas.v7i1.14268.

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There is a great interest on the strategical plan in Egypt for the cultivation of a new crop used as source of natural sweeteners Stevia rebaudiana (Spanti) and (China-1) var. Stem nodal segments containing axillary buds were used as an explant and inoculated on Murashige and Skoog’s (MS) medium containing 3% (w/v) sucrose, 0.8% (w/v) agar supplemented with various concentrations of flurprimidol (flur.), paclobutrazol (PBZ) and thidiazuron (TDZ). In Spanti var. maximum number of branches (6.52) and (6.4) were obtained in MS medium supplemented with 0.12 mg/l flur. and 0.2 mg/l TDZ, respectively with an average of 56.4 and 36.67 leaves per plantlet, having an average shoot length of 6.40 and 6.52 cm, respectively. The best in vitro root induction (100%) was achieved on MS medium with 0.16 mg/l flur. with an average of 10.00 roots per plantlet and root length of 4.82 cm (Spanti var.). Furthermore, in China-1 var. (MS) medium supplemented with 0.16mg/l flur. induced the best morphological characteristics. The rooted plantlets from explant planted on (MS) medium supplemented with 0.16 mg/l flur. were successfully established in soil and grown to maturity at the survival rate of 100% in the greenhouse in (Spanti) and (China-1) var. As a result of anatomy, all studied growth regulators significantly enhanced the anatomical characters of stevia varieties leaf and stem.flur.at 0.16 mg\l surpassed, for instance, midvein and lamina thickness, length and width of leaf vascular bundle as well as stem diameter, xylem and phloem thickness. Our data revealed that the numbers of protein bands in most of treatments are bigger than which in the control.

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Karim, MA, R. Jannat, MS Rahman, and MS Haque. "Micropropagation of Stevia Plant from Nodal Segments." Progressive Agriculture 19, no. 2 (November 9, 2013): 21–26. http://dx.doi.org/10.3329/pa.v19i2.16914.

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The experiment was conducted to develop and establish a reproducible protocol for plantlet regeneration in Stevia. Nodal cutting explants were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of ?-Napthaleneacetic acid (NAA) and 6-Benzylamino purine (BAP). The combination of NAA at 1.0 mgl-1 and BAP at 1.0 mgl-1 resulted in the highest percentage (100%) of callus initiation. The maximum shoot regeneration and development of shoot was observed at the same combination. The developed shoots from nodal cuttings, upon transfer to the MS medium containing indole butyric acid (IBA) at 0.1mgl-1 resulted in best rooting within 8 days.DOI: http://dx.doi.org/10.3329/pa.v19i2.16914 Progress. Agric. 19(2): 21 - 26, 2008

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32

Arya, Vinod, N. S. Shekhawat, and R. P. Singh. "Micropropagation of Leptadenia reticulata—A medicinal plant." In Vitro Cellular & Developmental Biology - Plant 39, no. 2 (March 2003): 180–85. http://dx.doi.org/10.1079/ivp2002394.

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Pellegrineschi, Alessandro, and David Tepfer. "Micropropagation and plant regeneration in Sesbania rostrata." Plant Science 88, no. 1 (January 1993): 113–19. http://dx.doi.org/10.1016/0168-9452(93)90116-h.

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Jones, Rodney O., Robert L. Geneve, and Sharon T. Kester. "Micropropagation of Gas Plant (Dictamnus albus L.)." Journal of Environmental Horticulture 12, no. 4 (December 1, 1994): 216–18. http://dx.doi.org/10.24266/0738-2898-12.4.216.

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Abstract Gas plant.(Dictamnus albus L.) was successfully micropropagated from greenhouse-grown plants capable of flowering. Stems from initial spring growth were surface disinfested and two- node sections were placed horizontally on modified Murishige and Skoog medium with 1 μM (0.2, ppm) benzyladenine (BA). A dose response to BA at 1,5 and 10 μM (0.2, 1.1,2.3 ppm) indicated that the greatest number of usable microshoots was observed in cultures from horizontally placed explants treated with 1 μM (0.2 ppm) BA. These cultures initiated between 7.6 and 9.5 shoots per culture with approximately 70% of the shoots greater than 2 cm (0.8 in). Microcuttings rooted poorly in vitro even with indolebutyric acid in the medium. However, microcuttings rooted between 71 and 86 percent under ex vitro conditions. Microcuttings either untreated or treated with indolebutyric acid (5,000 or 10,000 ppm) as a quick dip produced 1.8 to 2.6 roots per rooted cutting after being directly stuck in a peat-lite medium (Metro-Mix or Promix) in cell packs under high humidity. Untreated microcuttings rooted at higher percentages (67 to 88%) in peat-lite media compared to perlite or vermitculite (25 to 58%). Rooted microcuttings were successfully acclimatized to greenhouse conditions by gradually reducing humidity.

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Chen, Chiachung. "Cost analysis of plant micropropagation of Phalaenopsis." Plant Cell, Tissue and Organ Culture (PCTOC) 126, no. 1 (April 4, 2016): 167–75. http://dx.doi.org/10.1007/s11240-016-0987-4.

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Makunga, N. P., A. K. Jäger, and J. van Staden. "Micropropagation of Thapsia garganica—a medicinal plant." Plant Cell Reports 21, no. 10 (April 23, 2003): 967–73. http://dx.doi.org/10.1007/s00299-003-0623-8.

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Bablak, P., J. Draper, M. R. Davey, and P. T. Lynch. "Plant regeneration and micropropagation of Brachypodium distachyon." Plant Cell, Tissue and Organ Culture 42, no. 1 (July 1995): 97–107. http://dx.doi.org/10.1007/bf00037687.

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38

Abdalla, Neama, Hassan El-Ramady, Mayada K. Seliem, Mohammed E. El-Mahrouk, Naglaa Taha, Yousry Bayoumi, Tarek A. Shalaby, and Judit Dobránszki. "An Academic and Technical Overview on Plant Micropropagation Challenges." Horticulturae 8, no. 8 (July 25, 2022): 677. http://dx.doi.org/10.3390/horticulturae8080677.

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The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.

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Godbole, Manoj, Vanya K N, Lenifer H D, Amrutha Y, Shruthi K, and Sujay K. S. "Micropropagation of Pogostemon paniculatus." Asian Journal of Pharmaceutical Research and Development 10, no. 4 (August 13, 2022): 16–21. http://dx.doi.org/10.22270/ajprd.v10i4.1154.

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Plant regeneration potential from node & leaf explants of Pogostemon paniculatus through direct shoot proliferation was studied on Murashige and Skoog medium supplemented with various concentrations of cytokinins & auxins. As the seed germinability of the plant is low in vitro, we have tried these explants for micropropagation. Among different concentrations of individual hormones tested for nodal explants, benzylaminopurine (BAP) at 5µM & kinetin (Kn) at 0.5µM showed 4 & 2 mean number of shoots/explant respectively & combination of Kn (0.5 µM) + BAP (1.5 µM) showed 15 mean number of shoots/explant. For leaf explant combination of Kn (0.5 µM) + BAP (1.5 µM) showed 20 mean number of shoots/explant. Other hormones tested at all concentrations showed callus formation without the shoot development. Roots were established on the isolated shoots on naphthaleneacetic acid (NAA) at 0.25µM. Rooted plants were acclimatized. This is the first report of Pogostemon paniculatus micropropagation.

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McGuigan, Patrick J., Frank A. Blazich, and Thomas G. Ranney. "Micropropagation of Stewartia pseudocamellia." Journal of Environmental Horticulture 15, no. 2 (June 1, 1997): 65–68. http://dx.doi.org/10.24266/0738-2898-15.2.65.

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Abstract Single-node explants were excised from shoots of actively growing, 2-year-old seedlings of Stewartia pseudocamellia Maxim. (Japanese stewartia) on three dates associated with specific stock plant growth stages. Following surface sterilization, explants were placed on agar-solidified Woody Plant Medium (WPM) containing either no growth regulators or N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP) at 5.0 or 10.0 ppm (24.6 or 48.2 μM) or 0.025 or 0.05 ppm (0.11 or 0.23 μM) N-phenyl-N-1,2,3-thiadiazol-5-ylurea (TDZ). The most frequent budbreak was noted for explants placed on media containing 2iP at either concentration. Explants cultured at the softwood stage had less contamination and greater budbreak than explants taken from more mature stem tissue. In another study, the three distal, axillary nodes of each shoot were excised at 4-day intervals for 28 days beginning 52 days after stock plants were potted following cold storage at 7C (44F). Explants were surface sterilized and placed on WPM supplemented with 10 ppm (49.2 μM) 2iP either alone or in combination with 3 ppm (8.6 μM) gibberellic acid (GA3). Neither GA3 nor node position influenced budbreak frequency or shoot elongation. Days after potting (stock plant growth stage) influenced frequency of budbreak and shoot elongation with the optimal period for explant collection being 56 to 72 days after stock plants were potted. Elongated shoots (one microcutting per explant) were produced on both media. Microcuttings ≥10 mm (0.4 in) were rooted using ex vitro procedures and acclimatized to greenhouse conditions.

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Lineberger, Dan, David Reed, and Mary Rumpho. "Micropropagation of Camptotheca acuminata." HortScience 33, no. 4 (July 1998): 604c—604. http://dx.doi.org/10.21273/hortsci.33.4.604c.

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Camptotheca acuminata (Chinese happy tree, Nyssaceae) is a source of the anticancer phytochemical, camptothecin. An endangered species in its native China, Camptotheca has been grown in this country on an experimental basis, but the germplasm base is extremely narrow. As a prelude to the establishment of a plant improvement effort designed to increase the efficiency of camptothecin production, in vitro studies that will enable plant regeneration and shoot proliferation from selected clones have been undertaken. Shoot proliferating cultures were established from shoot tip explants and were maintained on WPM medium containing 4 μm BA. MS medium and nodal explants proved unsatisfactory. Shoot proliferation was highest when in vitro shoot tips were cultured on 4 μm BA compared to media containing no growth regulator or the cytokinins zeatin, thidiazuron, or kinetin. In vitro—produced shoot tips were rooted by direct sticking in plastic containers filled with RediEarth mix, and were successfully adapted to the greenhouse environment.

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Blazich, Frank A., and Juan R. Acedo. "Micropropagation of Flame Azalea." Journal of Environmental Horticulture 6, no. 2 (June 1, 1988): 45–47. http://dx.doi.org/10.24266/0738-2898-6.2.45.

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Shoots tips excised from an actively growing stock plant of flame azalea [Rhododendron calendulaceum (Michx.) Torr.] were surface sterilized, the terminal portions were removed (decapitated) and the shoots placed horizontally on agar-solidified Woody Plant Medium (WPM) supplemented with 15 ppm 6-(γ, γ-dimethylallylamino)-purine (2iP). Within 4 to 6 months multiple shoot formation commenced. After 2 to 3 additional months of growth, axillary shoots were excised from the original explants. The shoots were decapitated and placed on WPM. After 2 subcultures, 8-node axillary shoots were excised, decapitated and cultured on agar-solidified WPM supplemented with 0, 4, 8, 12, 16, 24, and 32 ppm 2iP. The greatest number of shoots (microcuttings) ≥ 5 mm (0.2 in) were produced at 12 ppm 2iP. Microcuttings ≥ 10 mm (0.4 in) were rooted using ex vitro procedures. Enhancement of both axillary shoot multiplication and shoot length was achieved by addition to the medium of 80 ppm adenine sulfate and 200 ppm NaH2PO4.

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Walia, N., S. Sinha, and S. B. Babbar. "Micropropagation of Crataeva nurvala." Biologia plantarum 46, no. 2 (September 1, 2003): 181–85. http://dx.doi.org/10.1023/a:1022882006682.

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Babber, S., K. Mittal, R. Ahlawat, and T. M. Varghese. "Micropropagation of Cardiospermum Halicacabum." Biologia plantarum 44, no. 4 (December 1, 2001): 603–6. http://dx.doi.org/10.1023/a:1013763208472.

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Da Silva, J. A. Teixeira, D. D. T. Giang, and M. Tanaka. "Photoautotrophic micropropagation of Spathiphyllum." Photosynthetica 44, no. 1 (March 1, 2006): 53–61. http://dx.doi.org/10.1007/s11099-005-0158-z.

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Patil, Vishwanath M. "Micropropagation studies inCeropegia SPP." In Vitro Cellular & Developmental Biology - Plant 34, no. 3 (July 1998): 240–43. http://dx.doi.org/10.1007/bf02822714.

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Iapichino, Giovanni, and Marcello Airò. "Micropropagation of Metrosideros excelsa." In Vitro Cellular & Developmental Biology - Plant 44, no. 4 (June 5, 2008): 330–37. http://dx.doi.org/10.1007/s11627-008-9127-0.

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Prakash, Surya, and Johannes Van Staden. "Micropropagation of Searsia dentata." In Vitro Cellular & Developmental Biology - Plant 44, no. 4 (July 9, 2008): 338–41. http://dx.doi.org/10.1007/s11627-008-9129-y.

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Kaliamoorthy, S., G. Naidoo, and P. Achar. "Micropropagation of Harpagophytum procumbens." Biologia plantarum 52, no. 1 (March 1, 2008): 191–94. http://dx.doi.org/10.1007/s10535-008-0043-2.

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Stojicic, D., D. Janosevic, B. Uzelac, V. Cokesa, and S. Budimir. "Micropropagation of Pinus peuce." Biologia plantarum 56, no. 2 (June 1, 2012): 362–64. http://dx.doi.org/10.1007/s10535-012-0099-x.

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Journal articles on the topic 'Plant micropropagation'

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