Relevant bibliographies by topics / Plant micropropagation

Academic literature on the topic 'Plant micropropagation'

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Published: 4 June 2021
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Journal articles on the topic "Plant micropropagation"

1

Giles, Kenneth L., and Walter M. Morgan. "Industrial-scale plant micropropagation." Trends in Biotechnology 5, no. 2 (February 1987): 35–39. http://dx.doi.org/10.1016/0167-7799(87)90035-7.

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RANCILLAC, M. J., and J. G. NOURRISSEAU. "MICROPROPAGATION AND STRAWBERRY PLANT QUALITY." Acta Horticulturae, no. 265 (December 1989): 343–48. http://dx.doi.org/10.17660/actahortic.1989.265.50.

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3

Clemente Muñoz, Margarita. "Micropropagation of endangered plant species." Ecologia mediterranea 21, no. 1 (1995): 291–97. http://dx.doi.org/10.3406/ecmed.1995.1779.

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R. E. Young, A. Hale, N. D. Camper, R. J. Keese, and J. W. Adelberg. "APPROACHING MECHANIZATION OF PLANT MICROPROPAGATION." Transactions of the ASAE 34, no. 1 (1991): 0328. http://dx.doi.org/10.13031/2013.31666.

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Kulchin, Yuriy Nikolaevich, Olga Valerievna Nakonechnaya, Irina Victorovna Gafitskaya, Olga Vadimovna Grishchenko, Tatyana Yuryevna Epifanova, Irina Yuryevna Orlovskaya, Yuriy Nikolaevich Zhuravlev, and Evgenii Petrovich Subbotin. "Plant Morphogenesis under Different Light Intensity." Defect and Diffusion Forum 386 (September 2018): 201–6. http://dx.doi.org/10.4028/www.scientific.net/ddf.386.201.

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The innovative LED light source (Sun Box) with irradiation spectrum close to the sun spectrum in the wavelength range 440-660 nm was used in experiment for study the influence of light intensity (75, 135, 230 and 382 μmol/s*m2) on the growth and development of plants. Standard fluorescent lighting was used as a control. The experiments were carried out on plantlets ofStevia rebaudianaandSolanum tuberosum, cvs. Snegir, Rozhdestvenskiy and Kamchatskii)in vitro. The illumination intensity of 75 and 230 μmol/s*m2promoted development ofS. rebaudianaplantlets with optimal values of morphometric parameters and well developed roots, which is important for plantlet adaptation to soil conditions. ForS. tuberosumplantlets (Snegir and Rozhdestvenskiy cultivars), radiation intensity of 135 μmol/s*m2was optimal for micropropagation. The illumination intensity of 230 μmol/s*m2led to a formation of plantlets with the largest total fresh mass among experimental groups. Sun Box light with intensity of 75 μmol/s*m2could be applicated for micropropagation of these cultivars: plantlets were the highest with the largest internodes number. Thus, the plant response to different light intensity was species-spesific, and – in case of potato plantlets – cultivar-spesific. The use of artificial light sources with distinct PPFD level could be preferable forS. tuberosumandS. rebaudianaplantlet micropropagationin vitro, as it could shorten the cultivation time, accelerate cultivation time, and reduce the cost of electricity.
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Singh, M., S. Sonkusale, Ch Niratker, and P. Shukla. "Micropropagation of Shorea robusta: an economically important woody plant." Journal of Forest Science 60, No. 2 (March 4, 2014): 70–74. http://dx.doi.org/10.17221/80/2013-jfs.

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Shorea robusta is a valuable tree species which provides good quality timber along with other useful materials like seeds which can be used as a source of starch. Woody plants are difficult to regenerate under in vitro conditions and only some success has been achieved so far. Here we have presented the data for successful in vitro regeneration of S. robusta using nodal explants. Shoot proliferation and rooting were also successfully achieved in subsequent subcultures. The best medium for shoot initiation and proliferation was found to be WPM with 1.0 mg&middot;l<sup>&ndash;1</sup> BAP and 0.5 mg&middot;l<sup>&ndash;1</sup> NAA and 1.0 mg&middot;l<sup>&ndash;1</sup> BAP +0.5 mg&middot;l<sup>&ndash;1</sup> NAA, respectively. Likewise for rooting WPM medium with 0.5 mg&middot;l<sup>&ndash;1</sup> IBA was found to be the best medium. &nbsp; &nbsp;
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7

Kozai, Toyoki. "Photoautotrophic micropropagation." In Vitro Cellular & Developmental Biology - Plant 27, no. 2 (April 1991): 47–51. http://dx.doi.org/10.1007/bf02632127.

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8

Onay, Ahmet, Hakan Yildirim, Yelda Ozden Tokatli, Hulya Akdemir, and Veysel Suzerer. "Plant tissue culture techniques—Tools in plant micropropagation." Current Opinion in Biotechnology 22 (September 2011): S130. http://dx.doi.org/10.1016/j.copbio.2011.05.426.

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9

Gargiulo, Jennifer A., and Michael E. Kane. "941 AQUARIUM PLANT MICROPROPAGATION: CRYPTOCORYNE BECKETII." HortScience 29, no. 5 (May 1994): 568e—568. http://dx.doi.org/10.21273/hortsci.29.5.568e.

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The genus Cryptocoryne (Araceae) contains some of the most commercially important amphibious species used in the aquarium plant trade. However, seed production is rare and vegetative propagation by rhizome division is extremely slow. Procedures for in vitro establishment, axillary shoot proliferation and plantlet acclimatization of Cryptocoryne Becketti Thwaites ex Trimen were determined. Surface sterilized rhizomatous shoot tips were established on a medium consisting of Linsmaier & Skoog mineral salts and organics supplemented with 87.6 mM sucrose, 2.2 μM benzyladenine (BA) and 0.57 μM indole-3-acetic acid (IAA) solidified with 0.8% TC® Agar. Effects of medium supplementation with factorial combinations of BA (0 - 25 μM) and IAA (0 - 10 μM) on axillary shoot proliferation from single node explants were determined after 28 days. Maximum axillary shoot proliferation (`l-fold increase) occurred on medium supplemented with 25 μM BA and 1.0 μM IAA. Excellent microcutting rooting (100%) was achieved by direct sticking in Vergro Klay Mix A. Greenhouse acclimatization of rooted microcuttings was 100%.
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10

El-Banna, H. "Micropropagation of thyme plant (Thymus vulgaris)." Journal of Plant Production 8, no. 11 (November 1, 2017): 1221–27. http://dx.doi.org/10.21608/jpp.2017.41294.

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Dissertations / Theses on the topic "Plant micropropagation"

1

Armitge, Neil. "Novel screening methods for plant micropropagation." Thesis, Durham University, 1991. http://etheses.dur.ac.uk/6288/.

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The techniques of plant micropropagation have not been successfully applied to all species. This study was carried out with the objectives of developing new techniques for rapidly assessing the relative merits of cultural treatments and identifying fundamental and genotype-specific problems associated with micropropagation. Anatomical characteristics of mlcropropagated Hosta spp. and Paeonia lactiflora were investigated. A root exodermis was present and apoplastic tracer studies indicated it was functionally and anatomically the same as ex vitro root exodermes in the literature. Specialised cells rather than simple wound tissue were present at the plantlet /medium interface An endodermls was present in the shoot base of Hosta plantlets. It is suggested that the basal zone of the shoot is functionally a specialised "root". It is hypothesised that carbohydrate status (or solute potential) of vascular cambla and/or root initial cell Is Important in the induction of adventitious root formation. Growth medium GA, and possibly raised inorganic phosphate, resulted in Increased shoot "health" but inhibited rooting in P. lactiflora cultures, possibly through changes in assimilate partitioning and sucrose uptake. A low mobility esterase isoenzyme was specific to these changes. Water relations are identified as a critical factor in the micropropagation of P. lactiflora. Cold storage of Hosta spp. led to sequential leaf senescence, abscission and changes in isoenzyme patterns. No true dormancy was identified in culture, although it was demonstrated after weaning if a requirement for cold storage was not met. In vitro, "dormancy" was expressed as a reduction in the rate of new leaf production. Removal of this growth inhibition was correlated with the appearance of a highly mobile esterase isoenzyme. The possibility of using this isoenzyme to predict subsequent in vitro growth inhibition and ex vitro dormancy is discussed. The objectives of this study were fulfilled, and the direction of future research is discussed.
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Taeb, Abdulkarim Giumaa. "Influence of culture environment on tulip micropropagation." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328788.

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Hudson, G. "Micropropagation and low temperature storage of Dieffenbachia." Thesis, University of East London, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370763.

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Newell, Christopher Jack. "In vitro soil-less (IVS) rooting medium." Thesis, Newell, Christopher Jack (2006) In vitro soil-less (IVS) rooting medium. PhD thesis, Murdoch University, 2006. https://researchrepository.murdoch.edu.au/id/eprint/227/.

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The principle hypothesis of this thesis is that hypoxia, in agar-based media, compromises rooting in vitro. From a practical point of view this is important because most plant tissue culture activities require the material to be successfully acclimatised in a nursery environment. Compromised rooting often results in excessive losses at this stage which are costly and inconvenient. In addition, many plants with commercial and/or scientific interest remain unavailable as they are not able to be rooted and acclimatised reliably. The use of agar as a rooting medium has limited the capacity of plant tissue culture to clonally propagate many plants. The thesis begins by demonstrating how poorly some plants respond to agar rooting media. Juvenile Chamelaucium hybrid microcuttings were pulsed with IBA 40 mcg M and then placed for 3 weeks on either M1 (1/2 MS) or aerated in vitro soil-less substrate (IVS) (Chapter 2). IVS had 42-82% rooting at the end of Stage 3 compared with 0-1% in agar. Shoot survival for IVS-rooted microcuttings was significantly greater than M1-rooted shoots. Pulsed shoots placed in IVS showed root primordia after 7 days. In contrast, shoots placed in agar showed no root primordia after 21 days and formed callus but did not root when subsequently placed in IVS for a further 4 weeks. The agar medium almost totally and permanently inhibited the capacity of competent shoots to form root primordia and roots. The effectiveness of different types of aerated and non-aerated media, including IVS, were tested to validate the hypothesis (Chapter 3). Microcuttings from shoot cultures of two Australian plants Grevillea thelemanniana and Verticordia plumosa x Chamelaucium uncinatum were pulsed for 7 days on a high auxin (40 mcg M IBA), agar-solidified medium in the dark. Rooting of the microcuttings was then compared on five experimental substrates: a) standard agar M1 medium (1/2 MS, no hormones, 8 g agar L-1), b) porous-agar medium (1/2 MS, no hormones, 30 g agar L-1, solidified then blended to provide aeration), c) white sand wet with liquid M1, d) white sand with M1 medium containing agar, and e) IVS. A separate experiment involved flushing the IVS soil profile with low or normal oxygen. Low and variable rooting percentages were recorded on the controls on M1 medium. Root induction and average total root length per microcutting at final harvest were significantly higher using the porous media including IVS, blended agar or white sand. The M1 medium and the addition of M1 medium to sand suppressed the percentage rooting and elongation. Flushing the IVS rooting medium with low oxygen also suppressed rooting. The experiments showed that increasing the air-filled porosity of the rooting medium has a positive effect on rooting and this is most likely due to the increased oxygen at the base of the microcutting. The role of ethylene, and the sugar and nutrients in M1 were not investigated. The efficacy of the IVS protocol on a range of Australian herbaceous and woody species was investigated to determine whether the observed benefits were generic or plant specific (Chapter 4). Improved rooting in IVS compared to agar was shown for 28 Australian species and genotypes from the families Liliaceae, Haemodoraceae, Myrtaceae, Thymelaeaceae, Proteaceae, Goodeniaceae and Rutaceae. Twenty-seven of the 28 species rooted in IVS medium at equal or better rates than in M1. In three cases - Actinodium cunninghamii, one of the Pimelea physodes genotypes and one of the Eriostemon australasius genotypes - shoots did not root in M1 but showed good root development in IVS medium. With few exceptions average root length and number in microcuttings rooted in IVS was superior to those in agar medium. To further test the resilience of the hypothesis, it was tested on nodal microcuttings of lentil which are recalcitrant to root in vitro (Chapter 5). The veracity of a published conclusion that inverted lentil microcuttings (with their base in the air) root better because of their altered polarity was also examined. It was found that, as is the case for many species, roots initiated and grew only at the proximal end of the microcutting regardless of its orientation. When the proximal end was in agar (a hypoxic environment) the rooting percentage was low (9-25%) even when the orientation of the microcutting was altered by inverting the culture tube. In contrast, when the proximal end of the microcutting was in an aerobic environment (from the shoot being placed upside down in agar medium or placed normally or upside down in an aerated medium) rooting percentages were higher (62-100%). Given that Stage 2 microcuttings are prepared with the objective to root and acclimatise them to nursery conditions, the duration of this activity becomes important as it can impact on plant quality and costs. The pulsing protocol and the length of time that Stage 3 cultures remain in the culture room during the rooting phase is a component of the unit cost of production of each rooted microcutting. Initially a 7-day IBA pulse was used after which the pulsed microcuttings were transferred to IVS to root. Chapter 6 shows that the pulsing period can be shortened to one day or replaced with a single auxin dip while still achieving high rooting percentages and maintaining plant quality. These materials handling improvements go some way to realising the logistical benefits of ex vitro rooting but without compromising the positive influences of hygiene and a stable environment of the in vitro environment.
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Newell, Christopher Jack. "In vitro soil-less (IVS) rooting medium." Newell, Christopher Jack (2006) In vitro soil-less (IVS) rooting medium. PhD thesis, Murdoch University, 2006. http://researchrepository.murdoch.edu.au/227/.

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The principle hypothesis of this thesis is that hypoxia, in agar-based media, compromises rooting in vitro. From a practical point of view this is important because most plant tissue culture activities require the material to be successfully acclimatised in a nursery environment. Compromised rooting often results in excessive losses at this stage which are costly and inconvenient. In addition, many plants with commercial and/or scientific interest remain unavailable as they are not able to be rooted and acclimatised reliably. The use of agar as a rooting medium has limited the capacity of plant tissue culture to clonally propagate many plants. The thesis begins by demonstrating how poorly some plants respond to agar rooting media. Juvenile Chamelaucium hybrid microcuttings were pulsed with IBA 40 mcg M and then placed for 3 weeks on either M1 (1/2 MS) or aerated in vitro soil-less substrate (IVS) (Chapter 2). IVS had 42-82% rooting at the end of Stage 3 compared with 0-1% in agar. Shoot survival for IVS-rooted microcuttings was significantly greater than M1-rooted shoots. Pulsed shoots placed in IVS showed root primordia after 7 days. In contrast, shoots placed in agar showed no root primordia after 21 days and formed callus but did not root when subsequently placed in IVS for a further 4 weeks. The agar medium almost totally and permanently inhibited the capacity of competent shoots to form root primordia and roots. The effectiveness of different types of aerated and non-aerated media, including IVS, were tested to validate the hypothesis (Chapter 3). Microcuttings from shoot cultures of two Australian plants Grevillea thelemanniana and Verticordia plumosa x Chamelaucium uncinatum were pulsed for 7 days on a high auxin (40 mcg M IBA), agar-solidified medium in the dark. Rooting of the microcuttings was then compared on five experimental substrates: a) standard agar M1 medium (1/2 MS, no hormones, 8 g agar L-1), b) porous-agar medium (1/2 MS, no hormones, 30 g agar L-1, solidified then blended to provide aeration), c) white sand wet with liquid M1, d) white sand with M1 medium containing agar, and e) IVS. A separate experiment involved flushing the IVS soil profile with low or normal oxygen. Low and variable rooting percentages were recorded on the controls on M1 medium. Root induction and average total root length per microcutting at final harvest were significantly higher using the porous media including IVS, blended agar or white sand. The M1 medium and the addition of M1 medium to sand suppressed the percentage rooting and elongation. Flushing the IVS rooting medium with low oxygen also suppressed rooting. The experiments showed that increasing the air-filled porosity of the rooting medium has a positive effect on rooting and this is most likely due to the increased oxygen at the base of the microcutting. The role of ethylene, and the sugar and nutrients in M1 were not investigated. The efficacy of the IVS protocol on a range of Australian herbaceous and woody species was investigated to determine whether the observed benefits were generic or plant specific (Chapter 4). Improved rooting in IVS compared to agar was shown for 28 Australian species and genotypes from the families Liliaceae, Haemodoraceae, Myrtaceae, Thymelaeaceae, Proteaceae, Goodeniaceae and Rutaceae. Twenty-seven of the 28 species rooted in IVS medium at equal or better rates than in M1. In three cases - Actinodium cunninghamii, one of the Pimelea physodes genotypes and one of the Eriostemon australasius genotypes - shoots did not root in M1 but showed good root development in IVS medium. With few exceptions average root length and number in microcuttings rooted in IVS was superior to those in agar medium. To further test the resilience of the hypothesis, it was tested on nodal microcuttings of lentil which are recalcitrant to root in vitro (Chapter 5). The veracity of a published conclusion that inverted lentil microcuttings (with their base in the air) root better because of their altered polarity was also examined. It was found that, as is the case for many species, roots initiated and grew only at the proximal end of the microcutting regardless of its orientation. When the proximal end was in agar (a hypoxic environment) the rooting percentage was low (9-25%) even when the orientation of the microcutting was altered by inverting the culture tube. In contrast, when the proximal end of the microcutting was in an aerobic environment (from the shoot being placed upside down in agar medium or placed normally or upside down in an aerated medium) rooting percentages were higher (62-100%). Given that Stage 2 microcuttings are prepared with the objective to root and acclimatise them to nursery conditions, the duration of this activity becomes important as it can impact on plant quality and costs. The pulsing protocol and the length of time that Stage 3 cultures remain in the culture room during the rooting phase is a component of the unit cost of production of each rooted microcutting. Initially a 7-day IBA pulse was used after which the pulsed microcuttings were transferred to IVS to root. Chapter 6 shows that the pulsing period can be shortened to one day or replaced with a single auxin dip while still achieving high rooting percentages and maintaining plant quality. These materials handling improvements go some way to realising the logistical benefits of ex vitro rooting but without compromising the positive influences of hygiene and a stable environment of the in vitro environment.
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6

Leifert, Carlo. "Contaminants of plant tissue cultures." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282645.

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Delaney, Belinda. "Verticordia micropropagation through direct ex vitro rooting." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2013. https://ro.ecu.edu.au/theses/615.

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The objective of this study was to improve the existing shoot multiplication protocol for Verticordia grandis (McComb, Arthur & Newll, 1986; Newell, Growns & McComb, 2005) and to investigate and establish reliable root induction and acclimatisation protocols to enhance survival of micropropagated plantlets. It was envisaged that these protocols would be successful in micropropagation, growth and survival of different V. grandis clones and possibly applicable to other Verticordia species. The elongation of in vitro Verticordia shoots on multiplication media was improved by reducing the concentration of BAP from 1μM to 0.25 μM, which resulted in a more uniform shoot length of 4.5 – 5 cm; necessary for root induction experiments. The root induction protocol was optimised by determining the appropriate auxin concentration (80μM indole butyric acid; IBA) with an exposure time of 6 days. Acclimatisation and survival was greatly improved by transferring the IBA pulsed shoots to ex vitro conditions consisting of a free draining and aerated substrate (a mixture of peat and perlite 1:3) in crack pots. These were initially placed into a greenhouse (with controlled temperature & light conditions) in order to maintain high humidity. Over time humidity was reduced and after 112 days the plantlets were transferred to larger pots, containing fresh soil (peat/perlite/sand = 1:1:1) and placed in a shade house with a regular watering regime. Long-term survival was monitored and after 252 days survival was over 70%. The declining survival rates after this time has made it evident that field performance and long-term survival needs further investigation. The application of the improved shoot multiplication and root induction protocols on other V. grandis clones produced survival rates of 0 to 62.5% (depending upon clone) over 252 days.
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Ofisi, Mbulelo. "In vitro propagation studies of rare Argyroderma species strictly endemic to the Knersvlakte region of South Africa." Thesis, Cape Peninsula University of Technology, 2017. http://hdl.handle.net/20.500.11838/2714.

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Thesis (MTech (Horticulture)--Cape Peninsula University of Technology, 2017.
A study was conducted to investigate the effects of various media composition and wounding treating on the in vitro propagation of Argyroderma subalbum and A. testiculare explants derived from mature plants, antioxidants and plant growth regulators (PGR) concentrations. One experiment consisted of 3 medium types including Murashige and Skoog (MS) medium strength, vitamin supplement. Fifteen replicates were used for each treatment. The shoots were then sub-cultured to ten replicate regenerated medium consisting of varying levels and combination of indole-3-acetic acid (IAA) and 10 μM 6-Benzyladenine (BA) supplements. In another experiment consisted of varying levels of auxins with MS medium strength, activated charcoal (AC) and vitamin supplements ten replicates were used for each treatment. Results indicated the positive role of cytokinins types’ 6-Benzyladenine (BA), 2-isopentyladenine (2iP) and Kinetin in inducing callus formation from wounded explants. The highest rate of friable callus formation of wounded explants was observed in media containing vitamin supplementation with BA at 10 μM. Callus formation significantly increased with the addition of vitamins at 10 μM on BA, 2iP and kinetin. With regards to the effects of various media composition and wounding explants on in vitro growth and regeneration of A. subalbum and A. testiculare, significant results were achieved with BA, 2iP and kinetin concentrations on explants discoloration and callus formation. The antioxidant treatment, AC did not reduce explants discoloration, but the induction of the callus was developed furthermore, results showed that IAA with BA concentrations without addition of AC there was significantly difference on both species but A. subalbum dominated with browning intensity (Chapter 3). Only sub-culturing of the explants succeeded in preventing explants discoloration and subsequently increased the number of shoots. The interaction between Indole-3-acetic acid (IAA) concentrations combined with BA resulted in the most effective technique in reducing explants discoloration at the media contact point. This study provides an insight into the contributing factor and methods of overcoming the major problem of phenolic oxidation and promoting the in vitro growth and regeneration of A. subalbum and A. testiculare.
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Mlungwana, Asanda. "In-vitro propagation studies of the endangered succulents Drosanthemum Micans and Drosanthemum Hallii (Aizoaceae)." Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2748.

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Thesis (MTech (Horticulture))--Cape Peninsula University of Technology, 2018.
Drosanthemum micans and Drosanthemum hallii are endangered succulent shrubs of horticultural and medicinal value. They are restricted to the Succulent Karroo, which is one of the world’s biodiversity hotspots. The species risk extinction from illegal over-harvesting for water-wise gardens, erosion by occasional flush floods from ephemeral rivers, competition from alien invasive species, overgrazing and clearing of land for agriculture and human settlement. Although seeds and cuttings may be used in propagating these species, they often require seasonal collection and planting and cuttings struggle to establish, hence the need for in-vitro propagation as an alternative solution. Thus, the main objective of the study was to develop a method for rapid in-vitro shoot and root multiplication and acclimatization of D. micans and D. hallii. To initiate shoot formation, disinfected leaf and stem nodal explants were cultured in Murashige and Skoog (1962) media supplemented with different rates (0, 10, 20 or 30μM) of 2-isopentyladenine, 6-Benzyladenine and kinetin for D. hallii or 2-isopentyladenine, 6-Benzyladenine and Thiadiazuron for D. micans. Shoots from explants were rooted in varying rates (0, 10, 20 or 30μM) of IAA for root initiation. Three media, which were used in previous studies, were tested for acclimatization of rooted explants in i) vermiculite, ii) sand (50%): vermiculite (50%) or iii) sand (75%): perlite (25%). For quantitative evaluation of plant stress, chlorophyll fluorescence index (Fv/Fm) was measured as a proxy for plant stressf stress. It emerged that stem nodal explants of D. hallii tend to produce multiple shoots whilst leaf explants tended to produce callus when cultured in full-strength Murashige and Skoog (1962). Shoot multiplication was optimal in both D. hallii and D. micans at 10 μM of kinetin. Root formation in both D. hallii and D. micans only occurred when shoots were transferred to a full-strength Murashige and Skoog (1962) media without any phytohormones added. The intensity of tissue browning increased at higher levels of cytokinins, suggesting an interaction of plant growth regulators with exudates from explants. Different acclimatization media tested showed no significant differences in the level of stress (Fv/Fm). It is recommended that Murashige and Skoog (1962) media with10 μM kinetin be used for shoot development and multiplication, followed by transfer of the shoots to fresh full-strength Murashige and Skoog (1962) media without hormones for root development. Acclimatization of the rooted explants was possible in one of the following media: i) vermiculite, ii) sand (50%): vermiculite (50%) or iii) sand (75%): perlite (25%) and in a misted greenhouse (ca. 60% RH), with gradual weekly reductions in humidity by 10% over 2 weeks.
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Zobayed, Sayed Md Akhter. "The use of natural pressurised forced ventilation in plant micropropagation." Thesis, University of Hull, 1996. http://hydra.hull.ac.uk/resources/hull:5898.

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A new, uncomplicated system for the forced ventilation of plants and cultures has been investigated in terms of both its efficiency of ventilation and its effects on the growth and physiology of various plant species, including cauliflower, tobacco, Annona (custard apple) and potato. This new system, which has no moving parts or artificial energy requirement, provides a sustained, pressurised stream of sterile, humidified air (RH = 70-94%) driven by humidity-induced diffusion. This process depends upon the maintenance of a gradient of water vapour across a microporous partition for inducing the diffusion of air into the apparatus. Flows up to 5 cm³ min¯¹ can be produced and the atmosphere in a 60 cm³ culture vessel can be renewed every 12 min Compared to the standard conventional diffusive method of ventilation, e. g. by capping the vessel with a polypropylene disc, this new system has proved to be 18X more efficient in removing accumulated ethylene and in keeping CO₂ and O₂ levels in culture vessels close to atmospheric. This forced ventilation system has also been shown to be very effective in the in vitro cultivation of seedlings or cuttings of cauliflower, tobacco, Annona and potato for improving growth and preventing symptoms of vitrification such as leaf epinasty, reduction of leaf area and production of abnormal stomata. In potato cuttings the induction and production of microtubers have been promoted and the growth of abnormal callus prevented. In Annona cuttings flower bud production, leaf and shoot growth and micropropagation have been promoted and leaf and flower bud abscission have been reduced. In cauliflower, tobacco and Annona the leaf chlorophyll contents, rates of photosynthesis and yields were improved by this forced ventilation. These beneficial effects have been variously attributed to the efficient removal of ethylene, the maintenance of near to atmospheric levels of CO₂ and O₂ by day and night and to the reduction of humidity levels in the vessels to below 100% RH. It is hoped that this new ventilation system, which is comparatively inexpensive and requires very little maintenance might have some useful applications in the field of tissue culture and perhaps particularly in developing countries.
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Books on the topic "Plant micropropagation"

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P, Debergh, and Zimmerman Richard H. 1934-, eds. Micropropagation: Technology and application. Dordrecht: Kluwer Academic, 1990.

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Bajaj, Y. P. S., 1936-, ed. High-tech and micropropagation V. Berlin: Springer, 1997.

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Bajaj, Y. P. S., 1936-, ed. High-tech and micropropagation VI. Berlin: Springer, 1997.

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Collin, Hamish A. Plant cell culture. Oxford: BIOS Scientific Publishers, 1998.

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Valle, Raymundo Enríquez del. Experiencias sobre propagación in vitro de plantas. Oaxaca: Instituto Tecnológico Agropecuario de Oaxaca, Centro de Investigación y Graduados Agropecuarios, 1994.

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6

Kyte, Lydiane. Plants from test tubes: An introduction to micropropagation. Portland, Or: Timber Press, 1987.

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7

G, Kleyn John, ed. Plants from test tubes: An introduction to micropropagation. 3rd ed. Portland, Or: Timber Press, 1996.

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8

Herman, Edwin B. Regeneration and micropropagation: Techniques, systems and media, 1997-1999. Shrub Oak [N.Y.]: Agritech Consultants, 2000.

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Herman, Edwin B. Regeneration and micropropagation: Techniques, systems and media, 1991-1995. Shrub Oak [N.Y.]: Agritech Consultants, 1995.

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Herman, Edwin B. Regeneration and micropropagation: Techniques, media and applications, 1999-2002. Shrub Oak [N.Y.]: Agritech Consultants, 2002.

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Book chapters on the topic "Plant micropropagation"

1

Preil, W. "Application of bioreactors in plant propagation." In Micropropagation, 425–45. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-009-2075-0_25.

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Bhojwani, Sant Saran, and Prem Kumar Dantu. "Micropropagation." In Plant Tissue Culture: An Introductory Text, 245–74. India: Springer India, 2013. http://dx.doi.org/10.1007/978-81-322-1026-9_17.

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Novak, F. J. "Micropropagation and plant tissue culture in developing countries of Africa." In Micropropagation, 205–13. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-2075-0_15.

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Honda, Hiroyuki, Chunzhao Liu, and Takeshi Kobayashi. "Large-Scale Plant Micropropagation." In Plant Cells, 157–82. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/3-540-45302-4_6.

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Bornman, C. H. "Micropropagation and somatic embryogenesis." In Plant Breeding, 246–60. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1524-7_17.

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Kaçar, Yıldız Aka, and Ben Faber. "Micropropagation of Banana." In Plant Cell Culture Protocols, 143–51. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-818-4_11.

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Singh, Aneesha. "Micropropagation of Plants." In Plant Biology and Biotechnology, 329–46. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2283-5_16.

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Preil, Walter. "Micropropagation of ornamental plants." In Plant Tissue Culture, 115–33. Vienna: Springer Vienna, 2003. http://dx.doi.org/10.1007/978-3-7091-6040-4_7.

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Piqueras, Abel, and Pierre C. Debergh. "Morphogenesis in Micropropagation." In Morphogenesis in Plant Tissue Cultures, 443–62. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-015-9253-6_15.

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López-Ramírez, Yessica, Alejandra Palomeque-Carlín, Lucía Isabel Chávez Ortiz, Ma de Lourdes de la Rosa-Carrillo, and Eugenio Pérez-Molphe-Balch. "Micropropagation of Yucca Species." In Plant Cell Culture Protocols, 171–77. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8594-4_10.

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Conference papers on the topic "Plant micropropagation"

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"Micropropagation of Threatened Betula Species for in vitro Conservation." In International Conference on Plant, Marine and Environmental Sciences. International Institute of Chemical, Biological & Environmental Engineering, 2015. http://dx.doi.org/10.15242/iicbe.c0115056.

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Orekhova, T. P. "Clonal micropropagation of Far Eastern tree species promising for plantation cultivation." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-326.

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Isakov, Igor, and Nadezhda Bokareva. "INTRODUCTION OF KARELIAN BIRCH TO THE CULTURE IN VITRO." In Modern problems of animal and plant ecology. FSBE Institution of Higher Education Voronezh State University of Forestry and Technologies named after G.F. Morozov, 2021. http://dx.doi.org/10.34220/mpeapw2021_5-9.

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Abstract:
At present, the biological diversity of tree species is drying up. One of the main reasons for extinction is the destructive anthropogenic impact. According to the latest data, it became known that the Karelian birch was included in the Red Book of the Republic of Karelia as an endangered and diminishing species. The in vitro clonal micropropagation technology can help to quickly restore the population of Karelian birch. And also the technology under consideration will help to massively produce seedlings and seedlings of Karelian birch for both decorative and silvicultural purposes.
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Nurwahyuni, Isnaini, and Riyanto Sinaga. "Micropropagation of Sumatra Benzoin (Styrax benzoin Dryander) to Obtain Plant Seedling." In International Conference of Science, Technology, Engineering, Environmental and Ramification Researches. SCITEPRESS - Science and Technology Publications, 2018. http://dx.doi.org/10.5220/0010067809570963.

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Solle, Hartini Realista Lydia, and Endang Semiarti. "Micropropagation of Sandalwood (Santalum album L.) endemic plant from East Nusa Tenggara, Indonesia." In TOWARDS THE SUSTAINABLE USE OF BIODIVERSITY IN A CHANGING ENVIRONMENT: FROM BASIC TO APPLIED RESEARCH: Proceeding of the 4th International Conference on Biological Science. Author(s), 2016. http://dx.doi.org/10.1063/1.4953500.

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Tao Zhang, Jing-long Liang, Yan Zou, and Ron-chong Li. "Micropropagation and bulblet growth of Lanzhou lily affected by plant growth regulators, sucrose and segments position." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5964101.

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Augustine, M. Sajimol, Lizzy Mathew, Roselin Alex, G. D. Deepa, and S. Jayalekshmi. "L-serine capped ZnS:Mn nanocrystals for plant cell biological studies and as a growth enhancing agent for micropropagation of Bacopa monnieri Linn. (Brahmi:Scrophulariaceae)." In OPTOELECTRONIC MATERIALS AND THIN FILMS: OMTAT 2013. AIP Publishing LLC, 2014. http://dx.doi.org/10.1063/1.4861995.

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Otte, Clemens, Joerg Schwanke, and Peter F. Jensch. "Automatic micropropagation of plants." In Photonics East '96, edited by George E. Meyer and James A. DeShazer. SPIE, 1996. http://dx.doi.org/10.1117/12.262874.

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"Micropropagation Of Commercially Important Ornamental Plants." In International Conference on Biological Research and Applied Science. Jinnah University for Women, Karachi,Pakistan, 2022. http://dx.doi.org/10.37962/ibras/2022/273-278.

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Batukaev, M. S., D. O. Palaeva, and A. A. Batukaev. "MICROPROPAGATION OF GRAPES IN VITRO." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-1172-1175.

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Reports on the topic "Plant micropropagation"

1

Young, Roy, Nahum Levav, Dwight Camper, Benjamin Steinitz, Bill Rhodes, and Itzhak Wolf. Alternative Techniques for Plant Micropropagation. United States Department of Agriculture, September 1993. http://dx.doi.org/10.32747/1993.7604301.bard.

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Guney, Murat, Mozhgan Zarifikhosrohahi, Songul Comlekcioglu, Hakan Keles, Muhammet Ali Gundesli, Ebru Kafkas, and Sezai Ercisli. Efficiency of Various Plant Growth Regulators on Micropropagation of Hawthorn (Crataegus spp.). "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, January 2020. http://dx.doi.org/10.7546/crabs.2020.01.07.

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