Journal articles on the topic 'Plant improvement (selection, breeding and genetic engineering)'
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1
Griesbach, R. J., P. Semeniuk M. Roh, and R. H. Lawson. "Tissue Culture in the Improvement of Eustoma." HortScience 23, no. 4 (August 1988): 658–791. http://dx.doi.org/10.21273/hortsci.23.4.658.
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Abstract Genetic engineering offers promise in the development of new plant forms. Alterations in plant size, branching, flower color and form, disease resistance, and many other factors may occur in plants produced by new technologies without sexual breeding and selection. Many desirable characteristics, not always observed in plants developed by breeding, are often expressed in plants engineered by tissue culture technology. Some of the plants showing these different new trait(s) also can be reproduced uniformly from seed, thus indicating genetic transmission of the new trait(s). This exciting approach to plant modification has been used to develop new forms of lisianthus.
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Mwamahonje, Andekelile, Deusdedit Kilambo, Leon Mrosso, and Tileye Feyissa. "Prospects of Biotechnology in Grape Breeding." JOURNAL OF ADVANCES IN AGRICULTURE 5, no. 1 (October 19, 2015): 565–73. http://dx.doi.org/10.24297/jaa.v5i1.4510.
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Genetic improvement of grape cultivars to obtain high quality wine and table grape varieties by conventional breeding methods has been difficult and time consuming. The elite grape varieties developed by conventional breeding techniques have less resistance to fungal and bacterial diseases, drought, quality and yield per plant. Breeding programs of grapes are difficult due to lack of true bred from seed and few traits of importance. Though most grapes constitute large number of genes, they have less effect in tolerating biotic and abiotic stresses. Genetic improvement of grapevine (Vitis vinifera L.) through application of biotechnological techniques provide new strategies in grape breeding programs based on rapid selection or induction of desired traits by marker assisted breeding, genetic engineering and plant tissue culture. This review paper therefore, aims to discuss biotechnological techniques proposed for improvement of grape breeding.
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Temesgen, Begna. "Speed breeding to accelerate crop improvement." International Journal of Agricultural Science and Food Technology 8, no. 2 (June 2, 2022): 178–86. http://dx.doi.org/10.17352/2455-815x.000161.
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Global food security has become a major issue as the human population grows and the environment changes, with the current rate of improvement of several important crops inadequate to meet future demand. Crop plants have extended generation times, which contributes to the slow rate of progress. However, speed breeding has revolutionized the entire world by reducing generation time and speeding up breeding and research programs to improve crop varieties. In the absence of an integrated pre-breeding program, breeding new and high-performing cultivars with market-preferred traits can take more than ten years. After the first cross with parental genotypes, a large amount of time, space, and resources are committed to the selection and genetic advancement stages during the early stages of breeding. Speed breeding has the ability to shorten the time it takes to develop, market, and commercialize cultivars. Crop improvement in the face of a fast-changing environment and an ever-increasing human population is a major concern for scientists around the world. Current crop enhancement projects are progressing at a rate that is insufficient to meet food demand. Crop redesign is urgently needed for climate resilience, as well as long-term yield and nutrition. Crop progress is slowed significantly by the long generation time required by crop plants during the breeding process. Speed breeding is now being used on a large scale to shorten generation time and support multiple crop generations per year as a solution in this approach. Researchers are now using an integrated approach to improve breeding efficiency, combining speed breeding with current plant breeding and genetic engineering methods. Speed breeding is a promising approach for achieving nutritional security and sustainable agriculture by shortening breeding cycles for food and industrial crop enhancement. Speed breeding is a methodology that allows plant breeders to improve crop production by adjusting temperature, light duration, and intensity to boost plant development. It uses an artificial source of light, which is kept on continuously, to activate the photosynthetic process, which leads to growth and reproduction much earlier than normal. This will assist in meeting the demands of the future’s rising population. This can be accomplished using a variety of technologies, including genotyping, marker-assisted selection, high throughput phenotyping; gene editing, genomic selection, and re-domestication, all of which can be combined with speed breeding to allow plant breeders to keep up with a changing climate and growing human population.
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Šušek, Andrej. "Perspectives of Christmas rose (Helleborus niger L.) genetic improvement." Agricultura 13, no. 1-2 (December 1, 2016): 11–19. http://dx.doi.org/10.1515/agricultura-2017-0003.
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Abstract The Christmas rose is becoming a very important ornamental plant on the market. It is relatively new to intensive production technologies. The number of genetically-improved varieties on the market is limited, and many of the old cultivars are no longer available because of slow and economically unfeasible vegetative propagation. In the future, its genetic improvement will probably become inevitable. New cultivars will have to satisfy the specific requirements of the market and producers as well as strict environmental policy. In order to obtain new cultivars it will be necessary to evaluate the existing germplasm and apply an adequate genetic breeding approach. There are seven main possibilities of creating new varieties: the use of natural variation associated with seed germination, population approach based on recurrent selection, individual selection based on specific genetic combinations, the creation of inbred lines and the formation of hybrids, interspecific hybridisation, mutagenesis and genetic engineering. Molecular markers can be very helpful during the breeding process. They can be used in order to study genetic relationships amongst populations, ecotypes, varieties, and hybrids.
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Kumar, Ravinder, Nand K. Sharma, Savita Meena, and Adithya P. Balakrishnan. "Mutation Breeding: A Way Forward for Genetic Improvement in Mungbean." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 7, no. 04 (December 31, 2021): 255–62. http://dx.doi.org/10.18811/ijpen.v7i04.3.
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Mungbean is a major food leguminous crop mainly cultivated in Asia. It is famous for its high protein, carbohydrate, and nutritional content. With the help of microorganisms located in their root nodules, the crop also maintain soil fertility through biological nitrogen fixation. This not only allows them to meet their own nitrogen needs, but it also improves the production of succeeding crops. One of the prerequisites for crop improvement is the availability of genetic variability. The capability to select improved genotypes in mungbean is limited by a lack of necessary diversity. Chemical and physical mutagens are frequently employed in Plant Mutation Breeding to boost crop productivity and resistance to diseases, insects, drought, and salt by creating genetic variability in crop plants. Mungbean is an early maturing crop often cultivated on low-fertility land with minimal inputs. In the case of these crops, the selection pressure has been focused on stress adaptation rather than yield. As a result, improving the genetics of such crops to increase yield necessitates genetic reconstitution to generate diverse plant types. Induced mutations can contribute to the regeneration and restoration of diversity that has been lost during the evolutionary process because of various pressures or adaptations. Thus, induced mutation or mutation breeding has a lot of potential for improving traditional agricultural crops like mungbean. In this paper, we look at many forms of mutations identified in mungbean crops by various scientists.
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Han, Fengqing, Yumei Liu, Zhiyuan Fang, Limei Yang, Mu Zhuang, Yangyong Zhang, Honghao Lv, Yong Wang, Jialei Ji, and Zhansheng Li. "Advances in Genetics and Molecular Breeding of Broccoli." Horticulturae 7, no. 9 (September 3, 2021): 280. http://dx.doi.org/10.3390/horticulturae7090280.
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Broccoli (Brassica oleracea L. var. italica) is one of the most important vegetable crops cultivated worldwide. The market demand for broccoli is still increasing due to its richness in vitamins, anthocyanins, mineral substances, fiber, secondary metabolites and other nutrients. The famous secondary metabolites, glucosinolates, sulforaphane and selenium have protective effects against cancer. Significant progress has been made in fine-mapping and cloning genes that are responsible for important traits; this progress provides a foundation for marker-assisted selection (MAS) in broccoli breeding. Genetic engineering by the well-developed Agrobacterium tumefaciens-mediated transformation in broccoli has contributed to the improvement of quality; postharvest life; glucosinolate and sulforaphane content; and resistance to insects, pathogens and abiotic stresses. Here, we review recent progress in the genetics and molecular breeding of broccoli. Future perspectives for improving broccoli are also briefly discussed.
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Penna, Suprasanna, and Shri Mohan Jain. "Fruit Crop Improvement with Genome Editing, In Vitro and Transgenic Approaches." Horticulturae 9, no. 1 (January 3, 2023): 58. http://dx.doi.org/10.3390/horticulturae9010058.
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Fruit species contribute to nutritional and health security by providing micronutrients, antioxidants, and bioactive phytoconstituents, and hence fruit-based products are becoming functional foods presently and for the future. Although conventional breeding methods have yielded improved varieties having fruit quality, aroma, antioxidants, yield, and nutritional traits, the threat of climate change and need for improvement in several other traits such as biotic and abiotic stress tolerance and higher nutritional quality has demanded complementary novel strategies. Biotechnological research in fruit crops has offered immense scope for large-scale multiplication of elite clones, in vitro, mutagenesis, and genetic transformation. Advanced molecular methods, such as genome-wide association studies (GWAS), QTLomics, genomic selection for the development of novel germplasm having functional traits for agronomic and nutritional quality, and enrichment of bioactive constituents through metabolic pathway engineering and development of novel products, are now paving the way for trait-based improvement for developing genetically superior varieties in fruit plant species for enhanced nutritional quality and agronomic performance. In this article, we highlight the applications of in vitro and molecular breeding approaches for use in fruit breeding.
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Gaba, Yashika, Ashwani Pareek, and Sneh Lata Singla-Pareek. "Raising Climate-Resilient Crops: Journey From the Conventional Breeding to New Breeding Approaches." Current Genomics 22, no. 6 (December 30, 2021): 450–67. http://dx.doi.org/10.2174/1389202922666210928151247.
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Background: In order to meet the demands of the ever-increasing human population, it has become necessary to raise climate-resilient crops. Plant breeding, which involves crossing and selecting superior gene pools, has contributed tremendously towards achieving this goal during the past few decades. The relatively newer methods of crop improvement based on genetic engineering are relatively simple, and targets can be achieved in an expeditious manner. More recently emerged genome editing technique using CRISPR has raised strong hopes among plant scientists for precise integration of valuable traits and removal of undesirable ones. Conclusion: Genome editing using Site-Specific Nucleases (SSNs) is a good alternative to the plant breeding and genetic engineering approaches as it can modify the genomes specifically and precisely at the target site in the host genome. Another added advantage of the genome editing approach is the simpler biosafety regulations that have been adopted by many countries for commercialization of the products thus generated. This review provides a critical assessment of the available methods for improving the stress tolerance in crop plants. Special emphasis has been given on genome editing approach in light of the diversity of tools, which are being discovered on an everyday basis and the practical applications of the same. This information will serve as a beginner’s guide to initiate the crop improvement programs as well as giving technical insight to the expert to plan the research strategically to tackle even multigenic traits in crop plants.
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Kulkarni, R. N., K. Baskaran, and Tripta Jhang. "Breeding medicinal plant, periwinkle [Catharanthus roseus(L) G. Don]: a review." Plant Genetic Resources 14, no. 4 (May 2, 2016): 283–302. http://dx.doi.org/10.1017/s1479262116000150.
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AbstractPeriwinkle [Catharanthus roseus(L) G. Don] has become one of the very extensively investigated medicinal plants after the discovery of two powerful anti-cancer alkaloids, vinblastine and vincristine, in its leaves more than 50 years ago. These alkaloidal drugs are still in clinical use. Also, periwinkle is still the only source of these alkaloids and their precursors, catharanthine and vindoline. Low concentrations of these alkaloids in the plant and, therefore, high costs of their extraction have led to tremendous efforts towards understanding their biosynthesis and exploration of alternate ways of their production such as, chemical synthesis, cell, tissue and hairy root cultures, and metabolic engineering of heterologous organisms. Literature on this plant is quite voluminous, with an average of about 80 publications per year during last three decades (1985–2015). Nearly 60% of these publications are on physiology, biochemistry, cell and tissue culture, phytochemistry, metabolic and genetic engineering aspects. In spite of these efforts, an economically viable alternative to field-grown periwinkle plants as a source of these alkaloids has not yet been found. Biosynthesis ofC. roseusalkaloids is a complex process involving many genes, enzymes, regulators, inter- and intra-cellular transporters, cell types, organelles and tissues and its current understanding is still considered to be incomplete to produceC. roseusalkaloids through metabolic engineering/synthetic biology. Till such time, breeding periwinkle varieties with higher concentrations of anti-cancer alkaloids for cultivation can be an alternate approach to meet the demand for these alkaloids and reduce their costs. While literature on cell and tissue culture, phytochemistry, metabolic and genetic engineering aspects of periwinkle has been reviewed periodically, crop production and plant breeding aspects have received little attention. In this paper, an attempt has been made to bring together published information on genetics and breeding of periwinkle as a medicinal plant. Some probable constraints which may have hindered taking up periwinkle breeding are identified. Initially, quite a few attempts have been made at genetic improvement of periwinkle through induced polyploidy, and subsequently through induced mutagenesis. Mutations, both natural and induced, provide a valuable resource for use in breeding and in functional and reverse genomics research. It is only during last 6–7 years, genetic diversity has been assessed using molecular markers and very recently molecular markers have been identified for marker-assisted selection for alkaloid yield.
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Trono, Daniela, and Nicola Pecchioni. "Candidate Genes Associated with Abiotic Stress Response in Plants as Tools to Engineer Tolerance to Drought, Salinity and Extreme Temperatures in Wheat: An Overview." Plants 11, no. 23 (December 2, 2022): 3358. http://dx.doi.org/10.3390/plants11233358.
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Wheat represents one of the most important staple food crops worldwide and its genetic improvement is fundamental to meeting the global demand of the growing population. However, the environmental stresses, worsened by climate change, and the increasing deterioration of arable land make it very difficult to fulfil this demand. In light of this, the tolerance of wheat to abiotic stresses has become a key objective of genetic improvement, as an effective strategy to ensure high yields without increasing the cultivated land. Genetic erosion related to modern agriculture, whereby elite, high-yielding wheat varieties are the product of high selection pressure, has reduced the overall genetic diversity, including the allelic diversity of genes that could be advantageous for adaptation to adverse environmental conditions. This makes traditional breeding a less effective or slower approach to generating new stress-tolerant wheat varieties. Either mining for the diversity of not-adapted large germplasm pools, or generating new diversity, are the mainstream approaches to be pursued. The advent of genetic engineering has opened the possibility to create new plant variability and its application has provided a strong complement to traditional breeding. Genetic engineering strategies such as transgenesis and genome editing have then provided the opportunity to improve environmental tolerance traits of agronomic importance in cultivated species. As for wheat, several laboratories worldwide have successfully produced transgenic wheat lines with enhanced tolerance to abiotic stresses, and, more recently, significant improvements in the CRISPR/Cas9 tools available for targeted variations within the wheat genome have been achieved. In light of this, the present review aims to provide successful examples of genetic engineering applications for the improvement of wheat adaptation to drought, salinity and extreme temperatures, which represent the most frequent and most severe events causing the greatest losses in wheat production worldwide.
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Li, Xiu-Qing. "Molecular characterization and biotechnological improvement of the processing quality of potatoes." Canadian Journal of Plant Science 88, no. 4 (July 1, 2008): 639–48. http://dx.doi.org/10.4141/cjps07086.
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Fries, chips, starches, flakes, and frozen pre-cooked potatoes, such as hash browns, are currently the main products made from processed potatoes. The processing quality of potatoes is largely determined by the carbohydrates, such as starch and glucose, in their tubers. The quantitative inheritance, heritability variation, transgene pleiotropic effects, and storage condition requirement of the processing quality are partly due to the carbohydrate metabolic network plasticity in potatoes. Pathway partition of sugars influences the processing quality such as chip color. This presentation attempts to review traditionally separate but related aspects (i.e., genetics, breeding, genomics, metabolism, marker-assisted selection, and transgenic engineering) of the potato processing quality and to discuss the integrated approach to its genetic improvement. Key words: Solanum tuberosum L., French fries, chips, carbohydrate metabolism, dry matter content, cold sweetening tuber morphology, metabolic network, partition of sugars, gene expression profile, transgenic regulation
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Isajev, Vasilije, Vladan Ivetic, Aleksandar Lucic, and Ljubinko Rakonjac. "Gene pool conservation and tree improvement in Serbia." Genetika 41, no. 3 (2009): 309–27. http://dx.doi.org/10.2298/gensr0903309i.
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This paper presents the concepts applied in the gene pool conservation and tree improvement in Serbia. Gene pool conservation of tree species in Serbia includes a series of activities aiming at the sustainability and protection of genetic and species variability. This implies the investigation of genetic resources and their identification through the research of the genetic structure and the breeding system of individual species. Paper also includes the study of intra- and inter-population variability in experiments - provenance tests, progeny tests, half- and full-sib lines, etc. The increased use of the genetic potential in tree improvement in Serbia should be intensified by the following activities: improvement of production of normal forest seed, application of the concept of new selections directed primarily to the improvement of only one character, because in that case the result would be certain, establishment and management of seed orchards as specialized plantations for long-term production of genetically good-quality forest seeds, and the shortening of the improvement process by introducing new techniques and methods (molecular markers, somaclonal variation, genetic engineering, protoplast fusion, micropropagation, etc.).
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Silva, Eduardo José de Souza, Crislaine Costa Calazans, Valdinete Vieira Nunes, and Renata Silva-Mann. "Estimative of royalties: appropriation of gains provided by innovations associated with plant breeding." Research, Society and Development 10, no. 13 (October 20, 2021): e513101321497. http://dx.doi.org/10.33448/rsd-v10i13.21497.
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Genetic breeding is based on the inheritance of quantitative traits, which provides the basis for developing methods that can be used to increase the rate of genetic improvement, known as genetic gain. Through selection, new varieties of plants can be obtained, which can be protected. Thus, this research investigates the mechanisms for estimating royalties in protected cultivars and the importance of genetic gains in such estimations. A systematic review was carried out based on articles published in 10 years, from 2009 to 2019, in the scientific bases Scopus (71) and Web of Science (73). The main crop, take as an example, was sugar cane. A survey of documents was carried out containing the Boolean terms and operators: “(genetic * and gain) and (sugarcane or cane)” in the title and/or abstract. One hundred forty-four articles were searched. After processing the data and removing duplicate documents, 107 scientific articles remained. With an average publication of 6.38 publications per year. Average of 14.6 citations per document. There were 463 authors and 456 co-authors, with nine documents having single authorship and an average of 4.33 authors per article. After the refinement process, a study sample of 25 articles was obtained. The analyzed journals provided information about the estimates, applied model, and study variables, being of great importance in constructing a linear regression model of royalty valuation linked to intellectual property rights related to the breeder of plant breeding.
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Afzal, Amir, Sairah Syed, Muhammad Saeed, Rabia Sultan, Misbah Kanwal, Moazan Shahid, Muhammad Zahid, and Basharat Mahmood. "Breeding Wheat for Rust Resistance: Conventional and Modern Approaches." Plant Protection 6, no. 3 (December 28, 2022): 285–98. http://dx.doi.org/10.33804/pp.006.03.4388.
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Three rusts are destructive, diminishing produce and nutritious value significantly, affect food availability and consequently food security through reductions in yield. In agricultural research institutes with mandate of wheat improvement, incorporating genes resistant against rust is matter of routine. The dilemma of rusts in wheat has been addressed the most, leading to discovery of principles of plant breeding for resistance e.g. gene disease genes inherit following Mendelian genetics, concept of genetic diversity and concept of gene for gene theory. Two strategies of breeding wheat for disease resistance are being followed. 1- Conventional and 2- Advanced. Among conventional approaches selection and hybridization are well known. However rust resistance has been found short lived and may also be durable in certain cases. Durability of disease resistance is desired and has been explored widely. Durability of resistance is generally attained through incorporation of genes effective at adult plant stage and combination of quantitative genes. Application of biotechnology to improve productivity of rust resistance breeding is the usage of molecular markers in pyramiding genes and substantiates the existence of genes in, and confirming released cultivars are pure. This involves molecular markers that are precise and pertinent across extensive ranges of breeders’ germplasm. This review article encompasses all features of wheat development through application of different techniques of wheat improvement. However, despite development of novel approaches that has accelerated wheat breeding, breeding in pathogen leading to producing more virulent strains. Consequently, wheat breeding is a continuous process.
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Ahmadikhah, Asadollah. "SELECTION EFFECTS IN RICE AS ASSESSED BY GENETIC ANALYSIS IN SEGREGATING POPULATIONS." Plant Breeding and Seed Science 77 (December 20, 2018): 69–77. http://dx.doi.org/10.37317/pbss-2018-0006.
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Estimation of selection effects on changes of a trait is of vital importance for the success of any plantbreeding program, and helps to select the desirable breeding method. Heritability and genetic advance areimportant selection parameters, and selection success is a reflectance of selection response. To estimate selectioneffects on rice genetic parameters, a research was conducted using 4 different generations (two parents:Neda and Sadri, and two segregating populations: BC1F1 and BC1S1). After development of a backcross population,a single plant (BC1#4) was selected based on its desirable performance, particularly in heading date andseven other morphological traits. BC1F1 population compared to mid-parent performance showed advance forheading date, plant height, tiller number, hundred seed weight, weight of filled seeds per panicle and grainyield per plant, while mean performance of BC1F1 population compared to BC1S1 population showed advanceonly for heading date, plant height, tiller number and grain yield. Prevalence of additive genetic effects incontrolling panicle weight, hundred seed weight, weight of filled seeds per panicle, plant height and headingdate was observed, and in contrast prevalence of non-additive effects in controlling grain yield was observed.High general heritability was observed for most traits, while only heading date and plant height showeda considerable specific heritability (60.7% and 67.5%, respectively), and grain yield showed a relatively lowspecific heritability (37.0%). High expected genetic advance (ΔGe) was obtained for tiller number (49.4%),followed by grain yield (43.5%) and plant height (35.5%), while the highest real genetic advance (ΔGe) wasobtained for heading date (-8.5%) and tiller number (5.4%). High selection success was obtained only forheading date (51.8%). Altogether, the obtained results gave promise for selecting progenies with early maturityand semi-dwarfism in early segregating generations, while they suggested preference of heterosis for improvementof grain yield.
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Dashora, A., R. Mehta, D. Singh, MISS Urmila, and S. K. Singh. "Genetic variability, association and diversity studies in wheat (Triticum spp. L.)." Journal of Environmental Biology 43, no. 03 (May 2, 2022): 390–400. http://dx.doi.org/10.22438/jeb/43/3/mrn-1972.
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Aim: The present study was aimed to assess the genetic variability and diversity in wheat genotypes and identification of elite lines for future genetic improvement. Methodology: Eighty-four wheat (Triticum spp. L.) genotypes were evaluated in Augmented Block Design with 5 blocks wherein each block contains 20 genotypes including 16 test entries and 4 checks (randomly allocated). Each genotype was sown in double rowed plot of 2.5 m. Results: High GCV and high genetic gain was observed for the traits like tillers/meter row length, grain yield per plot, spike length, grains per spike and 1000-grain weight showing predominance of additive genetic effect for these traits. Grain yield per plot had positive and significant correlation with plant height and tillers number per meter row length. Therefore, these characters should be emphasized more during selection for yield traits and thereby yield improvement in wheat. The cluster analysis identified DBW 107, KRL 283 among bread wheat and HI 8708 and HI 8765 among durum wheat as genetically most diverse genotypes. Interpretation: Greater emphasis should be given on tillers per meter row length and plant height traits while selecting for higher yield. Diverse genotypes identified by multivariate methods can be used in breeding program to generate transgressive segregants in wheat.
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Oram, Rex, and Greg Lodge. "Trends in temperate Australian grass breeding and selection." Australian Journal of Agricultural Research 54, no. 3 (2003): 211. http://dx.doi.org/10.1071/ar02137.
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Current trends in grass cultivar development are reviewed, with respect to the range of species involved, and the objectives and methodology within each species. Extrapolations and predictions are made about future directions and methodologies. It is assumed that selection will necessarily cater for the following environmental changes: (1) higher year-round temperatures, higher variability of rainfall incidence, and lower total winter and spring rainfall along the south of the continent; (2) higher nutrient and lime inputs as land utilisation intensifies; and (3) the grazing management requirements of the important pasture components will be increasingly defined and met in practice.The 'big four' species, perennial ryegrass, phalaris, cocksfoot and tall fescue, will continue to be the most widely sown species in temperate regions for many decades, with the latter 3 increasing most in area and genetic differentiation. However, species diversification will continue, especially with native grasses, legumes, and shrubs from fertile regions of Australia and exotics from little-explored parts of the world, such as South Africa, western North and South America, coastal Caucasus, and Iraq–Iran. By contrast, the recent high rate of species diversification in the tropics and subtropics will probably give way to a much lower rate of cultivar development by refinement and diversification within the established species. Domestication of native grasses will continue for amenity, recreational, land protection, and grazing purposes. As seed harvesting technologies and ecological knowledge improve, natural stands will become increasingly important as local sources of seed. It is suggested that many native grasses have been greatly changed by natural selection so as to withstand strong competition from introduced species under conditions of higher soil fertility and grazing pressure. Conversely, some introduced species are being selected consciously and naturally to persist in regions with irregular rainfall and less fertile soils. Therefore, the distinction between native and introduced grasses may be disappearing, and many populations of native species could now be as foreign to the habitats of pre-European settlement as are populations of introduced species that have been evolving here for 50–200 years. Methods used for genetic improvement will continue to be selection among both overseas accessions and the many native and introduced populations that have responded to natural selection in Australia. As well, there will be deliberate recurrent crossing and selection programs in both native and introduced species for specific purposes and environments. Increasingly, molecular biology methods will complement traditional ones, at first by the provision of DNA markers to assist the selection of complex traits, and for proving distinctness to obtain Plant Breeders' Rights for new cultivars. Later, genetic engineering will be used to manipulate nutritive value, resistance to fungal and viral diseases, and breeding systems, especially cytoplasmic male sterility and apomixis, to utilise heterosis in hybrid cultivars of grasses, particularly for dairying and intensive meat production.Areas where the practice and management of grass breeding and selection programs could be improved are highlighted throughout the review, and reiterated in a concluding statement. Most problems appear to stem from inadequate training in population ecology, population genetics, evolution, and quantitative inheritance.
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Goswami, Manika, Kaushal Attri, and Isha Goswami. "Applications of Molecular Markers in Fruit Crops: A Review." International Journal of Economic Plants 9, no. 2 (May 28, 2022): 121–26. http://dx.doi.org/10.23910/2/2022.0459.
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Markers are any trait of an organism that can be identified with confidence and relative ease, and can be followed in a mapping population or they can be defined as heritable entities associated with the economically important trait under the control of polygenes. Molecular markers have diverse applications in fruit crop improvement, particularly in the areas of genetic diversity and varietal identification studies, disease diagnostics, hybrid detection, sex differentiation and marker assisted selection. Molecular markers provide new directions to the efforts of plant breeders particularly in gene localization, taxonomy, phylogenetic analysis and also play an important role to decrease the time required for development of new and excellent cultivars. The most interesting application of molecular markers is marker-assisted selection (MAS). Suitable DNA markers should be polymorphic in the nature and should be expressed in all tissues, organs, at various developmental stages. Compared with traditional breeding programs, molecular markers can increase the efficiency and effectiveness of fruit breeding programs.
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Almeida, Patrícia de, Bruno Ettore Pavan, Maria Gabriela Fontanetti Rodrigues, Guilherme de Freitas Gaspar, Leonardo de Paula Giro, and Renan Furquim da Silva. "Genetic parameters and gains with the selection of fig tree genotypes." Acta Scientiarum. Agronomy 44 (June 29, 2022): e55796. http://dx.doi.org/10.4025/actasciagron.v44i1.55796.
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Fig tree (Ficus carica L., Moraceae), which originated in the Mediterranean Basin, is one of the many fruit trees grown in Brazil, with ‘Roxo-de-Valinhos’ being the exclusively used cultivar. In this context, research aimed at the improvement of this species to develop highly resistant and adaptable cultivars is paramount. Thus, the present study aimed to maintain fig accessions in an in vivo active germplasm bank (AGB) at the Faculty of Engineering of Ilha Solteira (FEIS), São Paulo State University (UNESP), as well as to characterize the agronomic traits of these accessions based on quantitative descriptors of genetic parameters and observe gains with the selection of specific genotypes to illustrate the AGB in terms of genetic variability. A total of 36 F. carica genotypes were evaluated in the field at the Teaching, Research and Extension Farm (FEPE) of the FEIS, UNESP. Qualitative traits, fruit parameters (e.g., insertion of the first fruit, fruit stalk length, fruit length, fruit diameter, and average fruit mass), and accumulated plant dry mass were measured. In addition, genetic parameters, variance components, and descriptive statistics, including genetic and environmental variances, heritability and average heritability of clones, coefficients of genotypic and environmental variation and their ratio ( ), general average, and selection gain, were evaluated. The selected fig tree accessions showed genetic variability in the assessed traits, exhibiting good heritability and achieving selection gains. For instance, the first 10 classified clones exhibited a heritability of 80.2% and achieved a selection gain of 98% for accumulated plant dry mass. Therefore, the maintenance of in vivo AGBs allows agronomic studies, offering promising results for continuing the breeding programs and preserving the genetic variability of species.
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Villao, Liliana, José Flores, and Efrén Santos-Ordóñez. "Genetic transformation of apical meristematic shoots in the banana cultivar ‘Williams’." Bionatura 6, no. 1 (February 15, 2021): 1462–65. http://dx.doi.org/10.21931/rb/2021.06.01.4.
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Bananas and plantains (Musa spp.) are among the most critical socioeconomic crops globally, being a staple food for millions of people in the tropics and an essential component for the export market, including the subtropics. Besides conventional breeding, genetic improvement of bananas and plantains could be performed through genetic engineering and new breeding techniques. Furthermore, plant tissue culture is essential for these technologies, including developing embryogenic cell suspensions and in vitro plants. The transient and stable genetic transformation could be performed from in vitro plants, shortening Musa transgenic lines development compared to genetic transformation while using embryogenic cell suspension. In this study, a genetic transformation protocol was established from banana apical meristems for the ‘Williams’ cultivar (genotype AAA). The protocol was based on the co-cultivation of the explants (whole in vitro plants or bisected meristematic tissues derived from in vitro plants) with Agrobacterium tumefaciens strain LBA4404 harboring two binary vectors denominated pLVCIBE1 (cassette: MabHIPP promoter::luc2::Tnos, P35S::hpt::Tnos) and pLVCIBE2 (cassette: P35S::luc2::Tnos, P35S::hpt::Tnos), independently. The stable genetic transformation was obtained by subculturing in vitro banana plants in selection medium (12.5µg/mL of hygromycin) for 8 weeks from bisected meristematic tissue transformation. Genetic transformation was confirmed in vivo with the use of the luciferase reporter gene system. Furthermore, PCR was performed on DNA extracted from leaves of regenerated transgenic in vitro plants after 8 weeks of selection, confirming stable genetic transformation. Therefore, genetic transformation was achieved in the apical meristematic tissue of in vitro banana plants with co-cultivation of Agrobacterium tumefaciens.
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K. C., Bigyan, Rishav Pandit, Bishnu Prasad Kandel, Kanchan Kumar K. C., Arpana K. C., and Mukti Ram Poudel. "Scenario of Plant Breeding in Nepal and Its Application in Rice." International Journal of Agronomy 2021 (June 30, 2021): 1–9. http://dx.doi.org/10.1155/2021/5520741.
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Rice, the number one staple food crop of Nepal, contributes nearly 20% to the agricultural gross domestic product, almost 7% to gross domestic product, and supplies with 40% of the food calorie consumption of Nepalese people. Despite of increasing production, the national demand of rice cannot be fulfilled, and billions of rupees are spent yearly for importing rice from India. This article reviews history, recent scenario, prospects, and importance of rice breeding research in Nepal for self-sufficiency. Though plant breeding inception point in Nepal was 1951, the systematic research in rice was started from 1972 after the establishment of the National Rice Research Program. Introduction and evaluation of high yielding exotic germplasm of rice was the initial rice breeding activities. After analysing several foreign materials, CH-45 variety of rice was first recommended in 1959. Nepal Agriculture Research Council (NARC), the leading organisation for rice varietal improvement to date, has been doing rice research activities such as germplasm conservation, exchange, varietal development, verification of technologies, and knowledge updates in collaboration with various organizations. International Rice Research Institute (IRRI), since 1966, has been playing an important role in Nepal’s rice research. Major plant breeding achievements are introduction of exotic materials and their evaluation, local landraces identification, collection and analysis, crossing of local landraces with exotic genetic materials, heterosis breeding, tissue culture technology, genetic marker technology, and National Genebank. RAPD and SSR, DNA-based markers, are mostly used for diversity appraisal and selecting desirable genotypes. Integration of advanced tools such as genetic engineering, bioinformatics, protoplast fusion, and SNP markers could not be still applied in rice research and development. Hence, emphasis should be given to improve the capacity and abilities of the rice scientists and should release more short duration, drought, and submergence-tolerant rice varieties by optimum utilisation of local landraces combining modern and conventional breeding.
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Qin, Guannan, Suting Wu, Liying Zhang, Yanyao Li, Chunmei Liu, Jianghui Yu, Lihua Deng, Guoying Xiao, and Zhiguo Zhang. "An Efficient Modular Gateway Recombinase-Based Gene Stacking System for Generating Multi-Trait Transgenic Plants." Plants 11, no. 4 (February 11, 2022): 488. http://dx.doi.org/10.3390/plants11040488.
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Transgenic technology can transfer favorable traits regardless of reproductive isolation and is an important method in plant synthetic biology and genetic improvement. Complex metabolic pathway modification and pyramiding breeding strategies often require the introduction of multiple genes at once, but the current vector assembly systems for constructing multigene expression cassettes are not completely satisfactory. In this study, a new in vitro gene stacking system, GuanNan Stacking (GNS), was developed. Through the introduction of Type IIS restriction enzyme-mediated Golden Gate cloning, GNS allows the modular, standardized assembly of target gene expression cassettes. Because of the introduction of Gateway recombination, GNS facilitates the cloning of superlarge transgene expression cassettes, allows multiple expression cassettes to be efficiently assembled in a binary vector simultaneously, and is compatible with the Cre enzyme-mediated marker deletion mechanism. The linked dual positive-negative marker selection strategy ensures the efficient acquisition of target recombinant plasmids without prokaryotic selection markers in the T-DNA region. The host-independent negative selection marker combined with the TAC backbone ensures the cloning and transfer of large T-DNAs (>100 kb). Using the GNS system, we constructed a binary vector containing five foreign gene expression cassettes and obtained transgenic rice carrying the target traits, proving that the method developed in this research is a powerful tool for plant metabolic engineering and compound trait transgenic breeding.
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Mohamad Ismail, Fatin N., Hazandy Abdul Hamid, Johar Mohamed, Arifin Abdu, Zaiton Samdin, and Sheriza Mohd Razali. "Character association and selection of breeding line based on morphophysiological characteristics and tensile strength in Hibiscus cannabinus L." BioResources 15, no. 4 (October 12, 2020): 8883–908. http://dx.doi.org/10.15376/biores.15.4.8883-8908.
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This study aimed to access different desirable characteristics of nine Hibiscus cannabinus L. accessions based on morphophysiological characteristics and fiber tensile strength for an effective selection of H. cannabinus plant improvement. Four China accessions (FH952, T15, T17, and T19), four Bangladesh accessions (HC2, HC95, V4202, and V4383), and V36 (control) accession were examined in a four-month cultivation period. The experimental design was arranged using randomized complete block design with three replications. Stem diameter was found to be significantly related (p ≤ 0.05) with all morphological and yield characteristics except for leaf dry weight and growth efficiency. Bigger stem diameter was an indicator of fiber yield in attempts to apply crossing and selection to improve performance. Photosynthesis rate also was found to be significantly related (p ≤ 0.05) with stomatal conductance, transpiration rate, instantaneous water use efficiency, and carboxylation efficiency. High photosynthesis rate could be an indicator to interpret the pattern of genetic variation of plant assimilation rate and its relation with environmental and agronomic factors. The fiber tensile modulus, however, was found to be inversely correlated with fiber diameter. The present study suggests the selection of control, V4383, HC2, and FH952 accessions for a breeding line as they possess high fiber yield, fiber strength, and photosynthetic efficiency.
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T. Popovski, Zoran, Blagica Tanaskovska, Elizabeta Miskoska – Milevska, Tome Nestorovski, Koco Porcu, Katerina Bandzo-Oreshkovikj, Milica Svetozarevic, Zimera Saiti, and Macdonald Wick. "APPLICATION OF MOLECULAR TOOLS IN ANIMAL BREEDING, CROP SCIENCE, FOOD CONTROL AND AGROBIODIVERSITY IN THE REPUBLIC OF MACEDONIA." Contributions, Section of Natural, Mathematical and Biotechnical Sciences 38, no. 2 (December 20, 2017): 165. http://dx.doi.org/10.20903/csnmbs.masa.2017.38.2.112.
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Molecular methods have become non-replaceable for the improvement of food production and its control. The Department for Biochemistry and Genetic Engineering (DBGE) as a part of the Faculty of Agriculture Sciences and Food is a crucial entity using molecular techniques in different areas of agriculture and livestock production. DBGE is mainly dealing with marker assisted selection (MAS), evolutionary studies; determine the origin of food products; enzyme analysis in food processing; transcriptional and translational analyses; molecular detection of plant viruses; and GMO analysis. The target molecules were proteins, RNA and DNA. MAS is systematically applied in animal breeding in Macedonia using RYR-1, k and αS1-casein genes. Evolutionary studies were performed using DNA microsatellites to estimate genetic distance among autochthonic strains of sheep and tomato varieties. The identification of food products origin and gender identification in fish was done using protein profiling. Gene expression was studied analysing different growth factors and inhibitors at RNA level. More than 3.000 plant and animal samples were analysed in the Laboratory for biochemistry and molecular biology. GMO laboratory as a part of the DBGE is the first authorized facility for GMO testing of food and feed samples. In more than 100 samples RT PCR methods were used.
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Shelenga, Tatyana V., Yulia A. Kerv, Irina N. Perchuk, Alla E. Solovyeva, Elena K. Khlestkina, Igor G. Loskutov, and Alexey V. Konarev. "The Potential of Small Grains Crops in Enhancing Biofortification Breeding Strategies for Human Health Benefit." Agronomy 11, no. 7 (July 15, 2021): 1420. http://dx.doi.org/10.3390/agronomy11071420.
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Nutrition is a source of energy, and building material for the human organism. The quality of food has an effect on the quality of individual life. Minerals and vitamins participate in various catalytic and regulatory functions of the main metabolic processes: absorption, transport, redox and biosynthesis of organic compounds, genetic information transfer, etc. Regular consumption of dietary fibers like β-glucans and oat-specific phenolics, antioxidants, and avenanthramides, stimulate innate and acquired immunity, prevent cancer, obesity, reduce glucose, total cholesterol and triglyceride blood levels and regulate the expression of cholesterol-related genes. Thus, all those compounds are vitally important for the normal functional status of the human body. A deficiency in one or another essential nutrient causes disruptions in human metabolism, thus leading to serious illnesses. Plants are the main source of essential nutrients that are bioavailable for humans. One of the most popular groups of staple crops are the small grains crops (SGC), so these crops are most often used for biofortification purposes. Exploiting the potential of plant resources, biofortification is a long-term strategy, aimed at increasing the number of essential micro- and macronutrients in major food sources and ensuring their bioavailability. The most productive way to implement such strategy is the active use of the possibilities offered by collections of plant genetic resources, including SGC, concentrated in various countries of the world. The collections of plant resources contain both cultivated plants and their wild relatives that possess the required composition of micro- and macronutrients. A complex scientific approach to studying plant germplasm collections, together with agricultural practices (soil enrichment with fertilizers with a required composition), genetic biofortification (traditional breeding, marker-assisted selection or genetic engineering tactics), and their combinations will lead to the development of new biofortified cultivars and improvement of old ones, which can be used to solve the problems of unbalanced nutrition (malnutrition or hidden hunger) in different regions of the world.
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Ahmed, F., M. Y. Rafii, M. R. Ismail, A. S. Juraimi, H. A. Rahim, R. Asfaliza, and M. A. Latif. "Waterlogging Tolerance of Crops: Breeding, Mechanism of Tolerance, Molecular Approaches, and Future Prospects." BioMed Research International 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/963525.
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Submergence or flood is one of the major harmful abiotic stresses in the low-lying countries and crop losses due to waterlogging are considerably high. Plant breeding techniques, conventional or genetic engineering, might be an effective and economic way of developing crops to grow successfully in waterlogged condition. Marker assisted selection (MAS) is a new and more effective approach which can identify genomic regions of crops under stress, which could not be done previously. The discovery of comprehensive molecular linkage maps enables us to do the pyramiding of desirable traits to improve in submergence tolerance through MAS. However, because of genetic and environmental interaction, too many genes encoding a trait, and using undesirable populations the mapping of QTL was hampered to ensure proper growth and yield under waterlogged conditions Steady advances in the field of genomics and proteomics over the years will be helpful to increase the breeding programs which will help to accomplish a significant progress in the field crop variety development and also improvement in near future. Waterlogging response of soybean and major cereal crops, as rice, wheat, barley, and maize and discovery of QTL related with tolerance of waterlogging, development of resistant variety, and, in addition, future prospects have also been discussed.
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Khuwaja, S., H. Bux, S. Abro, M. Rizwan, and G. M. Kaleri. "ESTIMATE OF HETEROSIS AND HETEROBELTIOSIS FOR THE IMPROVEMENT OF YIELD IN UPLAND COTTON (GOSSYPIUM HIRSUTUM L.)." Pakistan Journal of Agriculture, Agricultural Engineering and Veterinary Sciences 38, no. 1 (June 22, 2022): 1–6. http://dx.doi.org/10.47432/2022.38.1.1.
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The enhancing seed cotton yield per acre of the crop is a prime concern of breeding programmes. To achieve high degree of heterotic response, it is essential to have knowledge about performance of desirable parents . Therefore, research was undertaken to assess the expression of heterotic effects in cross combination of upland cotton in F1 crosses. The research work was carried out using six cross combinations sown under field condition during 2018 at Nuclear Institute of Agriculture, Tandojam experimental farm. The crosses were obtained by the combinations of four genotypes in half diallel method. The trial was conducted in a three replications with Randomized Complete Block Design (RCBD). Observations were made for plant height (cm), sympodial branches plant-1, number of bolls plant-1 , weight boll (g) and yield of plant-1 (g). Results showed that crosses exhibited significant cross vigour while compared with mid and better parents. The crosses Sadori × CRIS-342 and Sadori × NIA-Noori displayed significant heterotic effects for characters under studied. For development of hybrid in cotton, cross combinations of these parents Sadori × CRIS-342 and Sadori × NIA-Noori will give highest heterosis for yield of plant-1, bolls plant-1 and weight of bolls. Therefore, the above F1 transgressive and heterotic hybrids can be exploited to create genetic variability followed by selection of high yielding in cotton genotypes to establish strains with superior and improved characteristics in segregating filial generations.
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Sargar, Pramod R., Padmakar B. Wadikar, Sarvesh M. Shrotri, and G. R. Mahanthesh. "Generation Mean Analysis Studies in Safflower (Carthamus tinctures L.)." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 7, no. 03 (November 25, 2021): 240–42. http://dx.doi.org/10.18811/ijpen.v7i03.5.
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The present investigation was conducted at experimental research farm of Agriculture Botany College of Agriculture, Latur, in 2021, with a view to study the genetics of yield and yield components through generation mean analysis. The scaling test exhibited that, there was presence of epistatic gene interaction. The duplicate epistasis were observed in days to maturity and test weight in cross, JMU-1339 x NARI-6 and cross, JMU-1339 x EC-757665 for days to 50% flowering, number of branches per plant, number of seeds per capitulum and oil contents in cross-I; for plant height, number of capitulum per plant and hull contents in cross, JMU-1339 x EC-757665. This suggests the need of specific breeding procedure such as intermating of most desirable segregants followed by selfing and selecting superior genotypes coupled with progeny testing to exploit the population under study. Selection in early generation would be effective when additive effects are larger than non-additive ones. Further if the non-additive portions are larger than additive one, the improvement of the character need intensive selection through later generations.
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Evans, L. T. "Adapting and improving crops: the endless task." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 352, no. 1356 (July 29, 1997): 901–6. http://dx.doi.org/10.1098/rstb.1997.0069.
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The Malthusian prognosis has been undermined by an exponential increase in world food supply since 1960, even in the absence of any extension of the arable area. The requisite increases in yield of the cereal staples have come partly from agronomic intensification, especially of nitrogenous fertilizer use made possible by the dwarfing of wheat and rice, in turn made feasible by herbicide development. Cereal dwarfing also contributed to a marked rise in harvest index and yield potential. Although there is still scope for some further improvement in harvest index and environmental adaptation, it is not apparent how a doubling of yield potential can be achieved unless crop photosynthesis can be substantially enhanced by genetic engineering. Empirical selection for yield has not enhanced photosynthetic capacity to date, but nitrogenous and other fertilizers have done so, and there is still scope for agronomic increases in yield and for new synergisms between agronomy and plant breeding.
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Baiturina, Regina, Vladimir Konovalov, Aydar Gabdelkhakov, Elvira Khanova, and Dina Rafikova. "Forest Stand Reproduction in the Changing Climate Conditions on the Example of the Bashkortostan Republic." International Journal of Design & Nature and Ecodynamics 17, no. 6 (December 31, 2022): 891–98. http://dx.doi.org/10.18280/ijdne.170609.
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The paper considers the issues of forest reproduction by plantation type based on selection and genetic approaches in forest cultivation and forest seed production. The research explores the growth condition and regularities of common pine plus-trees, the best individuals with high-quality genetically determined traits for creating seed and vegetative seed plantations and further conducting breeding work are identified. There are findings on the genetic variability of trees on forest-seed plantations by ISSR markers on the example of common pine, identifying the existing gene pool of this species. Studies of forest reproduction by plantation type conducted throughout one republic or the planet as a whole provide preconditions for its improvement and higher efficiency of silvicultural works. Scientific and environmental institutions and authorities should join their efforts to develop effective measures to compensate for reforestation forest management.
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Paris, Harry S. "Consumer-oriented exploitation and conservation of genetic resources of pumpkins and squash, Cucurbita." Israel Journal of Plant Sciences 65, no. 3-4 (December 5, 2018): 202–21. http://dx.doi.org/10.1163/22238980-00001036.
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Pumpkins and squash, Cucurbita species, are valued horticultural products almost everywhere. They have been cultivated and subjected to consumer-oriented selection for thousands of years. Under this consumer orientation, they have been improved culinarily and diversified into the wonderful array of fruit sizes, shapes, and colors that are seen today. Besides their value as food items, pumpkins and squash are associated by people with abundance, warmth, sexuality, and life itself. My current objective is to provide a succinct perspective on the process of consumer-oriented exploitation of pumpkin and squash genetic resources. I briefly review the etymology, taxonomy and gross morphology of Cucurbita plants. A view is presented of how gathering, nurturing, domestication and cultivation of Cucurbita, species-specific and consumer-driven, maintained some of the parallels among species but also magnified the phenotypic differences among them. At greater length are considered the differences in resource allocation required for the preferential consumer-driven production of mature versus young fruits. Environmental effects, abiotic and biotic, are briefly mentioned, as are some of the potential benefits of biotechnology, genetic engineering, mapping, genomics, and gene editing as cognates for breeding. Finally, I consider the processes and needs for collection, maintenance, characterization, and availability of Cucurbita genetic resources and the dangers imposed by under-informed administrators in academia and cavalier governmental regulatory statutes toward future consumer-oriented improvement of pumpkins and squash.
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Singh, P. R., V. Mahajan, A. Verma, B. Lalramhlimi, Y. P. Khade, P. Gedam, N. Shukla, O. Sogam, and M. Singh. "Genetic variability, character association and path analysis for different traits in white onion genotypes under short day condition." Journal of Environmental Biology 43, no. 03 (May 2, 2022): 401–11. http://dx.doi.org/10.22438/jeb/43/3/mrn-1990.
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Aim: To estimate the nature and magnitude of genetic diversity of white onion genotypes. Methodology: The experiment was carried out for two consecutive years (2018-19 and 2019-20) at ICAR-DOGR, Pune. Thirty-five genotypes of white onion identified as low and high TSS genotypes were used to study their genetic variability, character association, path analysis and genetic diversity for different morphological and biochemical traits. Results: Significant differences were observed among all the genotypes. High expression of GCV over PCV was recorded for percentage double indicating lesser influence of environment. The broad sense heritability values were higher (60%) for all the characters under study ranging from 62.10% in days to 75% neck fall to 98.38% in total sugar. Plant height, leaf length, polar diameter and equatorial diameter exhibited significant positive correlation with total bulb yield. Genotypic path analysis showed that leaf length (cm) had maximum positive direct effect on total bulb yield followed by equatorial diameter, total sugar and total pyruvic acid of bulb. Interpretation: Considering the cluster distance and cluster means for bulb yield quality traits and morphometric traits three genotypes viz., Bhima Shweta, Bhima Shubra from cluster III and WHTB-7G-GT-15-SC-M-7 Small Bulb from cluster II were good candidates for utilization in breeding programs. Genotypes belonging to cluster I WHTS-4D-GT-18-MC-M-7 (18.49%), WHT-2B-GT-18-SC-M-7 (18.51%), WHTB-3C-GT-18-MC-M-7 (18.27%) and WHT-12L-HT-15-REJECT-M-7 (18.02%) can be utilized as high TSS donors in quality improvement programmes. This study also indicated that, direct selection of leaf length and equatorial diameter will be beneficial in framing yield improvement programme.
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Robinson, Terence. "Advances in apple culture worldwide." Revista Brasileira de Fruticultura 33, spe1 (October 2011): 37–47. http://dx.doi.org/10.1590/s0100-29452011000500006.
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Over the last 60 years, planting densities for apple have increased as improved management systems have been developed. Dwarfing rootstocks have been the key to the dramatic changes in tree size, spacing and early production. The Malling series of dwarfing rootstocks (M.9 and M.26) have been the most important dwarfing rootstocks in the world but are poorly adapted in some areas of the world and they are susceptible to the bacterial disease fire blight and the soil disease complex, apple replant disease which limits their uses in some areas. Rootstock breeding programs in several parts of the world are developing improved rootstocks with resistance to fire blight, and replant disease, and improved cold hardiness and yield efficiency. A second important trend has been the increasing importance of new cultivars. New cultivars have provided opportunities for higher prices until they are over-produced. A new trend is the "variety club" in which variety owners manage the production and marketing of a new unique cultivar to bring higher prices to the growers and variety owners. This has led to many fruit growers being unable to plant or grow some new cultivars. Important rootstock and cultivar genes have been mapped and can be used in marker assisted selection of future rootstock and cultivar selections. Other important improvements in apple culture include the development of pre-formed trees, the development of minimal pruning strategies and limb angle bending which have also contributed to the dramatic changes in early production in the 2nd-5th years after planting. Studies on light interception and distribution have led to improved tree forms with better fruit quality. Simple pruning strategies and labor positioning platform machines have resulted in partial mechanization of pruning which has reduced management costs. Improved plant growth regulators for thinning and the development of a thinning prediction model based on tree carbohydrate balance have improved the ability to produce the optimum fruit size and crop load. Other new plant growth regulators have also allowed control of shoot growth, control of preharvest fruit drop and control of fruit softening in storage after harvest. As we look to the future, there will be continued incremental improvement in our understanding of plant physiology that will lead to continued incremental improvements in orchard management but there is likely to be dramatic changes in orchard production systems through genomics research and genetic engineering. A greater understanding of the genetic control of dwarfing, precocity, rooting, vegetative growth, flowering, fruit growth and disease resistance which will lead to new varieties and rootstocks which are less expensive to grow and manage.
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Sankaran, Sindhuja, Afef Marzougui, J. Preston Hurst, Chongyuan Zhang, James C. Schnable, and Yeyin Shi. "Can High-Resolution Satellite Multispectral Imagery Be Used to Phenotype Canopy Traits and Yield Potential in Field Conditions?" Transactions of the ASABE 64, no. 3 (2021): 879–91. http://dx.doi.org/10.13031/trans.14197.
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HighlightsVegetation indices (NDVI, GNDVI, and SAVI) extracted from high-resolution satellite imagery were significantly associated with vegetation indices extracted from UAV imagery.High-resolution satellite data can be used to predict maize yield at breeding plot scale.Breeding plot sizes and the variability between maize genotypes may be associated with prediction accuracies.Abstract. The recent availability of high spatial and temporal resolution satellite imagery has widened its applications in agriculture. Plant breeding and genetics programs are currently adopting unmanned aerial vehicle (UAV) based imagery data as a complement to ground data collection. With breeding trials across multiple geographic locations, UAV imaging is not always convenient. Hence, we anticipate that, similar to UAV imaging, phenotyping of individual test plots from high-resolution satellite imagery may also provide value to plant genetics and breeding programs. In this study, high spatial resolution satellite imagery (~38 to 48 cm pixel-1) was compared to imagery acquired using a UAV for its ability to phenotype maize grown in two-row and six-row breeding plots. Statistics (mean, median, sum) of color (red, green, blue), near-infrared, and vegetation indices such as normalized difference vegetation index (NDVI), green normalized difference vegetation index (GNDVI), and soil adjusted vegetation index (SAVI) were extracted from imagery from both sources (UAV and satellite) for comparison at three time points. In general, a strong correlation between satellite and UAV imagery extracted NDVI, GNDVI, and SAVI features (especially with mean and median statistics, p < 0.001) was observed at different time points. The correlation of both UAV and satellite image features with yield potential was maximum (p < 0.001) at the third time point (milk/dough growth stages). For example, Pearson’s correlation coefficients between mean NDVI, GNDVI, and SAVI features with yield potential were 0.52, 0.54, and 0.51 for data derived from UAV imagery, and 0.34, 0.41, and 0.40 for data derived from satellite imagery, respectively. Machine learning algorithms, including least absolute shrinkage and selection operator (Lasso) regression, were evaluated for yield prediction using vegetation index features that were significantly correlated with observed yield. The relationship between satellite imagery with crop performance can be a function of plot size in addition to crop variability. Nevertheless, with the ongoing improvement of satellite technologies, there is a possibility for the integration of satellite data into breeding programs, thus improving phenotyping efficiencies. Keywords: Image processing, Machine learning, Plant breeding, Statistical analysis, Unmanned aerial vehicles.
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ULLAH, Mazhar, Mohammad S. KHAN, Asad JAN, Sayed U. A. SHAH, Navid IQBAL, and Iqbal MUNIR. "Biolistic transformation of sugarcane (Saccharum officinarum L.) with the Oryza sativa L. H+-PPase gene for improved salt stress tolerance." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 50, no. 4 (December 6, 2022): 12854. http://dx.doi.org/10.15835/nbha50312854.
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Several biotic and abiotic stresses adversely affect sugarcane crop. Amongst the abiotic stresses, salinity causes substantial losses to sugarcane yield. Lack of conducive environment for sugarcane flowering and unstable genome are the main constraints in sugarcane crop improvement via conventional breeding. Therefore, genetic engineering of sugarcane using Oryza sativa L. H+-PPase gene (OVP1) to confer salt tolerance is the preferred alternative strategy. In the present research, tissue culture and biolistic transformation protocols for sugarcane were optimized. For callus induction, Dichlorophenoxyacetic acid (2,4-D) was most effective. Maximum callus induction (50.22%) was achieved on CM-3 augmented with 2.5 mgL-1 2,4-D. The induced calli was subjected to biolistic transformation. For biolistic transformation, the gold particles (0.6 and 1.0 µm) and plasmid DNA (100 and 50 ng) were used for 1X and 2X bombardment. Significant differences (P≤0.05) were observed amongst the gold particles and plasmid DNA concentrations. Maximum transformation frequencies were observed when calli were bombarded 2X with 0.6 µm gold particles coated with 100 ng of DNA (23.60%). Selection and regeneration of transgenic calli were performed on MS media augmented with BAP (2.5 mgL-), NAA (1 mgL-1) hygromycin (25 mgL-1) and 2% coconut water. Regenerated plants after root induction on MS media containing NAA (2 mgL-1) and 2% coconut water were transferred to loam and clay (1:1) and acclimatized to greenhouse condition. The transgenic plants were confirmed through PCR and RT-PCR.
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Yang, Zishang, and Yuxing Han. "A Low-Cost 3D Phenotype Measurement Method of Leafy Vegetables Using Video Recordings from Smartphones." Sensors 20, no. 21 (October 25, 2020): 6068. http://dx.doi.org/10.3390/s20216068.
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Leafy vegetables are an essential source of the various nutrients that people need in their daily lives. The quantification of vegetable phenotypes and yield estimation are prerequisites for the selection of genetic varieties and for the improvement of planting methods. The traditional method is manual measurement, which is time-consuming and cumbersome. Therefore, there is a need for efficient and convenient in situ vegetable phenotype identification methods to provide data support for breeding research and for crop yield monitoring, thereby increasing vegetable yield. In this paper, a novel approach was developed for the in-situ determination of the three-dimensional (3D) phenotype of vegetables by recording video clips using smartphones. First, a smartphone was used to record the vegetable from different angles, and then the key frame containing the crop area in the video was obtained using an algorithm based on the vegetation index and scale-invariant feature transform algorithm (SIFT) matching. After obtaining the key frame, a dense point cloud of the vegetables was reconstructed using the Structure from Motion (SfM) method, and then the segmented point cloud and a point cloud skeleton were obtained using the clustering algorithm. Finally, the plant height, leaf number, leaf length, leaf angle, and other phenotypic parameters were obtained through the point cloud and point cloud skeleton. Comparing the obtained phenotypic parameters to the manual measurement results, the root-mean-square error (RMSE) of the plant height, leaf number, leaf length, and leaf angle were 1.82, 1.57, 2.43, and 4.7, respectively. The measurement accuracy of each indicators is greater than 80%. The results show that the proposed method provides a convenient, fast, and low-cost 3D phenotype measurement pipeline. Compared to other methods based on photogrammetry, this method does not need a labor-intensive image-capturing process and can reconstruct a high-quality point cloud model by directly recording videos of crops.
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Mühl, Marina. "Insect-Resistant Genetically Modified Crops: Regulation Framework and Current Situation in Argentina." Outlooks on Pest Management 31, no. 1 (February 1, 2020): 14–23. http://dx.doi.org/10.1564/v30_feb_04.
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Worldwide, there are many Insect-Resistant Genetically Modified Crops (IR-GMCs) planted with the purpose of controlling their many insect pests. All genetically modified (GM) plants have to pass through a regulatory system before being commercialized. In the case of Argentina, specific information is requested for these particular GM crops. This review will cover all the data required of IR-GMCs in Argentina in relation to insect resistance to the insecticidal products expressed (the most common in Argentina: Bt proteins) as well as the current situation of Bt crops in Argentina. From earliest times, man has used living organisms and their products in order to produce goods and services to meet their basic needs. For instance, man has modified, first unconsciously and then intentionally, the genome of many commodities so as to obtain improved cultivars. Taking the example of maize, its ancestor, the teocintle, is different in appearance (compared to the maize we consume nowadays). The selection process, which has taken place over many years, introduced improvement in many phenotypic characteristics such as the size of the grain. This example illustrates what is considered "Traditional Agrobiotechnology" or 'Traditional Plant Breeding'. The advent of Genetic Engineering and Molecular Biology in the second half of the 20th century has opened the door to "Modern Agrobiotechnology". The increase of agricultural production worldwide is demanded by a constantly increasing global population. As result of this, man has taken advantage of this valued tool so as to produce more in the same amount of land in a sustainable and cost-effective way. Thus different kinds of crops have been genetically engineered around the world with beneficial traits like insect resistance, herbicide tolerance and nutritional improvement. Worldwide, insects are a major cause of crop damage and yield loss, often requiring farmers to make multiple applications of chemical insecticides to control pests. For that reason, the commercial release of IR-GMCs also called Insect-resistant biotech crops has been an important contribution from Modern Agrobiotechnology to increase the global agricultural production. By the end of 2016, the cultivated area under GM crops reached 185.1 million hectares. 53% of that area was planted with IR-GMCs (single and stacked events with tolerance to herbicides). The commercialized genetically engineering crops that have protection against insect damage around the world are cotton, maize, soybean, potato, rice, tomato, eggplant and poplar. Insect-resistant biotech crops provide a number of benefits, such as a reduction in the use of chemical insecticides, improvement in yield, quality and lower production costs compared to the conventional crops.
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Bliss, Fredrick A. "Nutritional Improvement of Horticultural Crops through Plant Breeding." HortScience 31, no. 4 (August 1996): 696e—696. http://dx.doi.org/10.21273/hortsci.31.4.696e.
Full textAbstract:
Fruits and vegetables are rich sources of the micro mineral elements and vitamins often lacking in diets based on cereals, grain legumes, and starchy roots and tubers, but void of animal products. When embarking on a breeding program to improve nutritional compounds, the way the fruit or vegetable is consumed in mixed diets must be considered. To alleviate nutritional problems, the nutrients must not only be present in the plant parts consumed, but also absorbed efficiently in the body. In some cases, it may be necessary to modify compounds to improve absorption as well as increase the concentration. Breeding to improve nutritionally related traits can be approached in a manner similar to that for other traits; i.e., identification of genetic variability, selection for enhanced levels using either individual phenotype or family mean values, and testing for field performance. In addition to improving amount and availability, avoidance of undesirable correlated responses due to genetic or physiological linkages between the trait of interest and other traits deleterious to either plant growth or the consumer is critically important during selection. The growing number of molecular marker-based linkage maps should prove especially useful for identifying genes of interest and employing marker-aided selection. When insufficient variability for amount or type of compound is present in the gene pool, strategies using transgenic plants may be useful.
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Neyhart, Jeffrey L., Aaron J. Lorenz, and Kevin P. Smith. "Multi-trait Improvement by Predicting Genetic Correlations in Breeding Crosses." G3: Genes|Genomes|Genetics 9, no. 10 (July 29, 2019): 3153–65. http://dx.doi.org/10.1534/g3.119.400406.
Full textAbstract:
The many quantitative traits of interest to plant breeders are often genetically correlated, which can complicate progress from selection. Improving multiple traits may be enhanced by identifying parent combinations – an important breeding step – that will deliver more favorable genetic correlations (rG). Modeling the segregation of genomewide markers with estimated effects may be one method of predicting rG in a cross, but this approach remains untested. Our objectives were to: (i) use simulations to assess the accuracy of genomewide predictions of rG and the long-term response to selection when selecting crosses on the basis of such predictions; and (ii) empirically measure the ability to predict genetic correlations using data from a barley (Hordeum vulgare L.) breeding program. Using simulations, we found that the accuracy to predict rG was generally moderate and influenced by trait heritability, population size, and genetic correlation architecture (i.e., pleiotropy or linkage disequilibrium). Among 26 barley breeding populations, the empirical prediction accuracy of rG was low (-0.012) to moderate (0.42), depending on trait complexity. Within a simulated plant breeding program employing indirect selection, choosing crosses based on predicted rG increased multi-trait genetic gain by 11–27% compared to selection on the predicted cross mean. Importantly, when the starting genetic correlation was negative, such cross selection mitigated or prevented an unfavorable response in the trait under indirect selection. Prioritizing crosses based on predicted genetic correlation can be a feasible and effective method of improving unfavorably correlated traits in breeding programs.
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Řepková, J., and J. Nedělník. "Modern methods for genetic improvement of Trifolium pratense." Czech Journal of Genetics and Plant Breeding 50, No. 2 (June 12, 2014): 92–99. http://dx.doi.org/10.17221/139/2013-cjgpb.
Full textAbstract:
This review focuses on trends in genetic improvement of a significant representative forage crop, Trifolium pratense (red clover) classified taxonomically into the agronomically outstanding family Fabaceae. Red clover breeding is aimed at improving traits like persistency, resistance to biotic and abiotic factors, forage yield and quality characteristics such as protein quality and stability. Isoflavone content in forage is important for cattle reproduction. Interspecific hybridization of red clover with the related wild species T. medium was used for the introgression of useful traits into red clover. The breeding strategy for the new variety Pramedi included hybrid plants with different DNA contents, repeated backcrosses with red clover, followed by family selection. New techniques of molecular genetics are becoming available to breeders for transferring key and complex traits into improved red clover varieties. Techniques such as marker-assisted selection and candidate gene identification can increase the speed and precision with which traits may be selected. Comparative sequence data analyses helped to identify genes for polyphenol oxidase enzymes and isoflavone synthase in red clover useful for DNA genotyping of individuals and breeding of improved high-quality red clover varieties.
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Zambelli, A. "THE IMPACT OF MOLECULAR GENETICS IN PLANT BREEDING: REALITIES AND PERSPECTIVES." Journal of Basic and Applied Genetics 30, no. 1 (July 2019): 11–15. http://dx.doi.org/10.35407/bag.2019.xxx.01.02.
Full textAbstract:
Even when conventional breeding was effective in achieving a continuous improvement in yield, Molecular Genetics tools applied in plant breeding contributed to maximize genetic gain. Thus, the use of DNA technology applied in agronomic improvement gave rise to Molecular Breeding, discipline which groups the different breeding strategies where genotypic selection, based on DNA markers, are used in combination with or in replacement of phenotypic selection. These strategies can be listed as: marker-assisted selection; marker-assisted backcrossing; marker assisted recurrent selection; and genomic selection. Strong arguments have been made about the potential advantages that Molecular Breeding brings, although little has been devoted to discussing its feasibility in practical applications. The consequence of the lack of a deep analysis when implementing a strategy of Molecular Breeding is its failure, leading to many undesirable outcomes and discouraging breeders from using the technology. The aim of this work is to trigger a debate about the convenience of the use of Molecular Breeding strategies in a breeding program considering the DNA technology of choice, the complexity of the trait of agronomic interest to be improved, the expected accuracy in the selection, and the demanded resources. Key words: DNA marker, selection, plant improvement.
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Pepó, P. "Improvement of effectiveness in maize breeding." Acta Agronomica Hungarica 54, no. 3 (September 1, 2006): 351–58. http://dx.doi.org/10.1556/aagr.54.2006.3.10.
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Plant regeneration via tissue culture is becoming increasingly more common in monocots such as maize (Zea mays L.). Pollen (gametophytic) selection for resistance to aflatoxin in maize can greatly facilitate recurrent selection and the screening of germplasm for resistance at much less cost and in a shorter time than field testing. In vivo and in vitro techniques have been integrated in maize breeding programmes to obtain desirable agronomic attributes, enhance the genes responsible for them and speed up the breeding process. The efficiency of anther and tissue cultures in maize and wheat has reached the stage where they can be used in breeding programmes to some extent and many new cultivars produced by genetic manipulation have now reached the market.
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Greveniotis, Vasileios, and Vasilia A. Fasoula. "Application of prognostic breeding in maize." Crop and Pasture Science 67, no. 6 (2016): 605. http://dx.doi.org/10.1071/cp15206.
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Innovative approaches and new efficiencies in plant breeding are required to accelerate the progress of genetic improvement through selection. One such approach is the application of prognostic breeding, which is an integrated crop-improvement methodology that enables selection of plants for high crop yield potential by evaluating its two components: plant yield potential and stability of performance. Plant yield and stability are assessed concurrently in each generation by utilising the plant prognostic equation. The genetic material used for this study was 2350 F2 plants (C0) of the commercial maize hybrid Costanza. The study presents the results of the application of prognostic breeding for 6 years in two contrasting environments (A and B), starting from C0 and ending in C5. It utilises ultra-high selection pressures (1.5% to 0.5%) to isolate superior lines with crop yield comparable to Costanza, and estimates the annual genetic gain accomplished through application of this selection strategy. Application of prognostic breeding led to the isolation of superior lines whose productivity was comparable to Costanza. The productivity gap between Costanza and the best selection was reduced from 87% (C0) to 0.5% (C5) in trial 1 (environment A), from 87% (C0) to 2% (C5) in trial 2 (environment B) and from 70% (C0) to 1% (C3) in trial 3 (environment B). Genetic gain was much higher (up to 50%) in the early cycles C0–C2 of prognostic breeding and smaller in cycles C3–C5. The best lines selected were evaluated in randomised complete block trials across both environments and 2 years. Across years, the top two lines in environments A and B averaged 87% and 91% of the Costanza yield, respectively, and they had higher prolificacy (greater number of ears per plant) than Costanza. Across all cycles, the average annual genetic gain ranged from 23% to 36% in the different trials, providing evidence that selection efficiency can be significantly maximised by using this breeding strategy.
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Moniruzzaman, M., R. Khatun, and AA Mintoo. "Application of marker assisted selection for livestock improvement in Bangladesh." Bangladesh Veterinarian 31, no. 1 (March 31, 2015): 1–11. http://dx.doi.org/10.3329/bvet.v31i1.22837.
Full textAbstract:
Molecular markers usually do not have any biological effect. They are identifiable DNA sequences, found at specific locations of the genome, and transmitted from one generation to the next. Marker assisted selection (MAS) is a novel technique that can complement traditional breeding methods for rapid genetic gains. Genetic gain through selective breeding is the objective of a breeder to achieve long term improvement in animal and plant genomes; however the pace of improvement is inversely proportional to the Generation Interval. Genetic improvement in livestock, particularly those with long generation intervals, requires decades for tangible results. Successful MAS breeding programmes require gene mapping, marker genotyping, quantitative trait loci (QTL) detection, genetic evaluation and finally MAS. Genomic selection is a form of markerassisted selection. Using markers covering the whole genome could mean potentially that all the genetic variance is explained; and the markers are assumed to be in linkage disequilibrium with the QTL so that the number of effects per QTL to be estimated is small. MAS drastically reduces generation interval and increases selection accuracy. Therefore, a breeding strategy based upon markers making the best use of the two approaches can facilitate rapid genetic gain though selection of markers related to economic traits such as milk and meat production. This review is designed to elaborate the technique of MAS and its application in developing countries. DOI: http://dx.doi.org/10.3329/bvet.v31i1.22837 Bangl. vet. 2014. Vol. 31, No. 1, 1-11
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Poudel, Kabita, and Parbati Thapa. "GENOMIC SELECTION: FUTURE OF POTATO CROP IMPROVEMENT." Tropical Agroecosystems 2, no. 2 (June 30, 2021): 91–95. http://dx.doi.org/10.26480/taec.02.2021.91.95.
Full textAbstract:
Crop improvement through plant breeding is fundamental to meet the food demand of the increasing population estimated to reach more than 9 billion by 2050. Potato is the third most important food crop globally and is considered imperative for global food security. The demand for improved potato cultivars for enhanced yield, quality, and disease resistance is increasing. Conventional potato breeding is less efficient and time-consuming, which takes more than a decade to improve desirable traits in the selected population. However, Genomic selection (GS) is evolving as an efficient tool for potato breeding which provides opportunities to increase the genetic gain of complex traits per unit time and cost. The genomic prediction model has shown promising results in various traits like yield, maturity, processing qualities, and diseases resistance. This study is designed to understand the basics of GS and the application of genomic selection in potato crop improvement.
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Barrett, Brent, Marty Faville, Alieu Sartie, David Hume, Zulfi Jahufer, Michael Hickey, Ivan Baird, et al. "Forage Improvement via Marker-Assisted Selection." NZGA: Research and Practice Series 12 (January 1, 2006): 11–15. http://dx.doi.org/10.33584/rps.12.2006.3030.
Full textAbstract:
The use of DNA markers to accelerate genetic improvement of forages presents a unique set of opportunities, challenges, and benefits. Our experiments in full-sib mapping populations of white clover and perennial ryegrass have detected >75 quantitative trait loci (QTLs), each with multiple marker:trait associations at specific locations in either the perennial ryegrass or white clover genome. A subset of these QTL are robust (detected in multiple years / sites / populations) and exert a substantial influence on performance, warranting exploration of development for application in Marker-assisted Selection (MAS) breeding programmes. Ryegrass QTLs associated with herbage yield, seed yield, plant size and habit, cold tolerance, seasonal regrowth, and disease have been identified, whereas QTL discovery in white clover has been focused on reproductive traits. Markers from two white clover QTLs were used to develop marker assays suitable for selection of parental plants with superior breeding value for seed yield potential. Tandem testing of the two assays over two field seasons and eight populations indicates that substantial change in seed yield may be achieved (up to 90% increase), and that the marker / allele / phase relationships to plant performance are population specific. These data point to an opportunity to develop selection tools on a population specific basis, and to a challenge to implement MAS approaches tailored for open-pollinated population breeding systems.
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Forster, B. P., M. A. Lee, U. Lundqvist, S. Millam, K. Vamling, and T. M. A. Wilson. "Genetic engineering of crop plants: from genome to gene." Experimental Agriculture 33, no. 01 (January 1997): 15–33. http://dx.doi.org/10.1017/s0014479797000136.
Full textAbstract:
Genetic engineering of crop plants has been in progress since the dawn of agriculture, about 10 000 years ago. For millennia the genetic make-up of our crop plants has been changed by mankind's selection of naturally occurring variants. As the trade routes were developed, novel plant types were introduced into new environments and provided more variation from which to choose. At the end of the nineteenth century an understanding of the laws of heredity was gained and plant breeding protocols were devised whereby selection became accompanied by deliberate crossing. As the knowledge of the genetic structure of crop plants improved, new ways of manipulation were invented and exploited. Indeed plant breeding became a testing bed for new ideas in genetics. For the plant breeder the techniques which were most widely employed in the past were those which aided breeding, for example techniques which speeded up the production of new varieties, but still used traditional routes of crossing and selection. This was a transitional phase between plant breeding as an art and plant breeding as a science.
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Kannenberg, L. W., and D. E. Falk. "Models for activation of plant genetic resources for crop breeding programs." Canadian Journal of Plant Science 75, no. 1 (January 1, 1995): 45–53. http://dx.doi.org/10.4141/cjps95-008.
Full textAbstract:
Although plant breeders recognize that their working germplasm is constricted, most make little use of the extensive genetic variability in gene banks and other collections. This is because breeders continue to make reasonable progress in most crop species and broadening the activated genetic base generally will dilute agronomic performance. Yet new germplasm can (1) raise the genetic ceiling on improvement, (2) decrease vulnerability to biotic and abiotic stresses, and (3) add new developmental pathways and ecological adaptations. Two breeding systems — HOPE for corn and RIPE for barley — are described which can significantly broaden the genetic base deployed in breeding programs while still producing lines and cultivars with commercial potential. The Hierarchical Open-ended Population Enrichment (HOPE) breeding system consists of two complementary sets, each of four gene pools arranged in a hierarchy based on agronomic performance. Introductions are added continually and desirable genes and gene complexes can routinely move upward in the hierarchy. Increasingly stringent selection procedures at each higher level of the hierarchy shape the extensive variability at the lower levels into the quality germplasm at the Elite level, which serves as the source of inbred lines. The Recurrent Introgressive Population Enrichment (RIPE) breeding system for barley employs male sterile facilitated recurrent selection to progressively introgress new genes and gene complexes into an Elite population which serves as the source of potential new cultivars. Key words: Broad-based breeding system, HOPE (maize), hierarchical open-ended system, RIPE (barley), recurrent introgressive selection, male sterile facilitated selection
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Sandhu, Karansher Singh, Aalok Shiv, Gurleen Kaur, Mintu Ram Meena, Arun Kumar Raja, Krishnapriya Vengavasi, Ashutosh Kumar Mall, et al. "Integrated Approach in Genomic Selection to Accelerate Genetic Gain in Sugarcane." Plants 11, no. 16 (August 17, 2022): 2139. http://dx.doi.org/10.3390/plants11162139.
Full textAbstract:
Marker-assisted selection (MAS) has been widely used in the last few decades in plant breeding programs for the mapping and introgression of genes for economically important traits, which has enabled the development of a number of superior cultivars in different crops. In sugarcane, which is the most important source for sugar and bioethanol, marker development work was initiated long ago; however, marker-assisted breeding in sugarcane has been lagging, mainly due to its large complex genome, high levels of polyploidy and heterozygosity, varied number of chromosomes, and use of low/medium-density markers. Genomic selection (GS) is a proven technology in animal breeding and has recently been incorporated in plant breeding programs. GS is a potential tool for the rapid selection of superior genotypes and accelerating breeding cycle. However, its full potential could be realized by an integrated approach combining high-throughput phenotyping, genotyping, machine learning, and speed breeding with genomic selection. For better understanding of GS integration, we comprehensively discuss the concept of genetic gain through the breeder’s equation, GS methodology, prediction models, current status of GS in sugarcane, challenges of prediction accuracy, challenges of GS in sugarcane, integrated GS, high-throughput phenotyping (HTP), high-throughput genotyping (HTG), machine learning, and speed breeding followed by its prospective applications in sugarcane improvement.
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Delgado, Jhon Paul Mathews, Francisco Célio Maia Chaves, Ricardo Lopes, Carlos Meneses, Magno Sávio Ferreira Valente, Filipe Almendagna Rodrigues, Moacir Pasqual, Santiago Ferreyra Ramos, Ananda Virginia de de Aguiar, and Maria Teresa Gomes Lopes. "Indirect Selection for Seed Yield in Sacha-Inchi (Plukenetia volubilis) in Brazil." Horticulturae 8, no. 11 (October 25, 2022): 988. http://dx.doi.org/10.3390/horticulturae8110988.
Full textAbstract:
Breeding programs for improvement of sacha-inchi, Plukenetia volubilis L., generally aim to select individuals with greater seed yield since there is a strong correlation between seed yield and oil production. However, the manual removal of seed husks for evaluating this trait is laborious and costly, thereby discouraging breeding efforts. Accordingly, the objective of the present study was to estimate gains from indirect selection of seed production in sacha-inchi progenies, focusing on maximizing efficiency in improvement programs. Genetic parameters along with direct and indirect selection gains were estimated for seed yield traits in 12 open-pollinated progenies. Strong genetic correlations were observed between total number of fruits (TNF), total weight of fruits (TWF), and total weight of seeds (TWS) per plant (r > 0.96). Notably, all three traits demonstrated high heritability (h2 > 0.81). Therefore, plants with high TNF and TWF (Cuzco, Dos de Mayo, Shanao, Aucaloma, and AM-7) can be used to indirectly select the genetic traits of higher seed yields (GS% = 23%). Genetic gain for dry seed production with a selection index of 42% was estimated at 23%, which corresponds to 118 kg.ha−1. Future sacha-inchi improvement programs can select progenies with high TNF and TWF to facilitate the selection of progenies with high TWS.