Log in

Relevant bibliographies by topics / Plant gene silencing

Contents

Journal articles
Dissertations / Theses
Books
Book chapters
Conference papers

Academic literature on the topic 'Plant gene silencing'

Author: Grafiati

Published: 4 June 2021

Last updated: 4 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Plant gene silencing.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Plant gene silencing"

1

Richards, Kenneth E. "Plant Gene Silencing." Plant Science 162, no. 4 (April 2002): 643. http://dx.doi.org/10.1016/s0168-9452(02)00006-7.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Unver, Turgay, and Hikmet Budak. "Virus-Induced Gene Silencing, a Post Transcriptional Gene Silencing Method." International Journal of Plant Genomics 2009 (June 15, 2009): 1–8. http://dx.doi.org/10.1155/2009/198680.

Full text

Abstract:

Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA-mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence-specific manner. Therefore, different types of methodologies have been advanced and modified for VIGS approach. Virus-derived inoculations are performed on host plants using different methods such as agro-infiltration and in vitro transcriptions. VIGS has many advantages compared to other loss-of-gene function approaches. The approach provides the generation of rapid phenotype and no need for plant transformation. The cost of VIGS experiment is relatively low, and large-scale analysis of screening studies can be achieved by the VIGS. However, there are still limitations of VIGS to be overcome. Nowadays, many virus-derived vectors are optimized to silence more than one host plant such as TRV-derived viral vectors which are used for Arabidopsis and Nicothiana benthamiana. By development of viral silencing systems monocot plants can also be targeted as silencing host in addition to dicotyledonous plants. For instance, Barley stripe mosaic virus (BSMV)-mediated VIGS allows silencing of barley and wheat genes. Here we summarize current protocols and recent modified viral systems to lead silencing of genes in different host species.

APA, Harvard, Vancouver, ISO, and other styles

3

Bruening, G. "Plant gene silencing regularized." Proceedings of the National Academy of Sciences 95, no. 23 (November 10, 1998): 13349–51. http://dx.doi.org/10.1073/pnas.95.23.13349.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Senior, Ian J. "Uses of Plant Gene Silencing." Biotechnology and Genetic Engineering Reviews 15, no. 1 (April 1998): 79–120. http://dx.doi.org/10.1080/02648725.1998.10647953.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Land, K. "Gene silencing and plant antiviral immunity." Trends in Genetics 17, no. 7 (July 1, 2001): 379. http://dx.doi.org/10.1016/s0168-9525(01)02404-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Bucher, Etienne, Titia Sijen, Peter de Haan, Rob Goldbach, and Marcel Prins. "Negative-Strand Tospoviruses and Tenuiviruses Carry a Gene for a Suppressor of Gene Silencing at Analogous Genomic Positions." Journal of Virology 77, no. 2 (January 15, 2003): 1329–36. http://dx.doi.org/10.1128/jvi.77.2.1329-1336.2003.

Full text

Abstract:

ABSTRACT Posttranscriptional silencing of a green fluorescent protein (GFP) transgene in Nicotiana benthamiana plants was suppressed when these plants were infected with Tomato spotted wilt virus (TSWV), a plant-infecting member of the Bunyaviridae. Infection with TSWV resulted in complete reactivation of GFP expression, similar to the case for Potato virus Y, but distinct from that for Cucumber mosaic virus, two viruses known to carry genes encoding silencing suppressor proteins. Agrobacterium-based leaf injections with individual TSWV genes identified the NSS gene to be responsible for the RNA silencing-suppressing activity displayed by this virus. The absence of short interfering RNAs in NSS-expressing leaf sectors suggests that the tospoviral NSS protein interferes with the intrinsic RNA silencing present in plants. Suppression of RNA silencing was also observed when the NS3 protein of the Rice hoja blanca tenuivirus, a nonenveloped negative-strand virus, was expressed. These results indicate that plant-infecting negative-strand RNA viruses carry a gene for a suppressor of RNA silencing.

APA, Harvard, Vancouver, ISO, and other styles

7

Qiao, Yongli, Rui Xia, Jixian Zhai, Yingnan Hou, Li Feng, Yi Zhai, and Wenbo Ma. "Small RNAs in Plant Immunity and Virulence of Filamentous Pathogens." Annual Review of Phytopathology 59, no. 1 (August 25, 2021): 265–88. http://dx.doi.org/10.1146/annurev-phyto-121520-023514.

Full text

Abstract:

Gene silencing guided by small RNAs governs a broad range of cellular processes in eukaryotes. Small RNAs are important components of plant immunity because they contribute to pathogen-triggered transcription reprogramming and directly target pathogen RNAs. Recent research suggests that silencing of pathogen genes by plant small RNAs occurs not only during viral infection but also in nonviral pathogens through a process termed host-induced gene silencing, which involves trans-species small RNA trafficking. Similarly, small RNAs are also produced by eukaryotic pathogens and regulate virulence. This review summarizes the small RNA pathways in both plants and filamentous pathogens, including fungi and oomycetes, and discusses their role in host–pathogen interactions. We highlight secondarysmall interfering RNAs of plants as regulators of immune receptor gene expression and executors of host-induced gene silencing in invading pathogens. The current status and prospects of trans-species gene silencing at the host–pathogen interface are discussed.

APA, Harvard, Vancouver, ISO, and other styles

8

Zhang, Huan, Gozde S. Demirer, Honglu Zhang, Tianzheng Ye, Natalie S. Goh, Abhishek J. Aditham, Francis J. Cunningham, Chunhai Fan, and Markita P. Landry. "DNA nanostructures coordinate gene silencing in mature plants." Proceedings of the National Academy of Sciences 116, no. 15 (March 25, 2019): 7543–48. http://dx.doi.org/10.1073/pnas.1818290116.

Full text

Abstract:

Delivery of biomolecules to plants relies onAgrobacteriuminfection or biolistic particle delivery, the former of which is amenable only to DNA delivery. The difficulty in delivering functional biomolecules such as RNA to plant cells is due to the plant cell wall, which is absent in mammalian cells and poses the dominant physical barrier to biomolecule delivery in plants. DNA nanostructure-mediated biomolecule delivery is an effective strategy to deliver cargoes across the lipid bilayer of mammalian cells; however, nanoparticle-mediated delivery without external mechanical aid remains unexplored for biomolecule delivery across the cell wall in plants. Herein, we report a systematic assessment of different DNA nanostructures for their ability to internalize into cells of mature plants, deliver siRNAs, and effectively silence a constitutively expressed gene inNicotiana benthamianaleaves. We show that nanostructure internalization into plant cells and corresponding gene silencing efficiency depends on the DNA nanostructure size, shape, compactness, stiffness, and location of the siRNA attachment locus on the nanostructure. We further confirm that the internalization efficiency of DNA nanostructures correlates with their respective gene silencing efficiencies but that the endogenous gene silencing pathway depends on the siRNA attachment locus. Our work establishes the feasibility of biomolecule delivery to plants with DNA nanostructures and both details the design parameters of importance for plant cell internalization and also assesses the impact of DNA nanostructure geometry for gene silencing mechanisms.

APA, Harvard, Vancouver, ISO, and other styles

9

Rodríguez-Negrete, Edgar A., Jimena Carrillo-Tripp, and Rafael F. Rivera-Bustamante. "RNA Silencing against Geminivirus: Complementary Action of Posttranscriptional Gene Silencing and Transcriptional Gene Silencing in Host Recovery." Journal of Virology 83, no. 3 (November 19, 2008): 1332–40. http://dx.doi.org/10.1128/jvi.01474-08.

Full text

Abstract:

ABSTRACT RNA silencing in plants is a natural defense system mechanism against invading nucleic acids such as viruses. Geminiviruses, a family of plant viruses characterized by a circular, single-stranded DNA genome, are thought to be both inducers and targets of RNA silencing. Some natural geminivirus-host interactions lead to symptom remission or host recovery, a process commonly associated with RNA silencing-mediated defense. Pepper golden mosaic virus (PepGMV)-infected pepper plants show a recovery phenotype, which has been associated with the presence of virus-derived small RNAs. The results presented here suggest that PepGMV is targeted by both posttranscriptional and transcriptional gene silencing mechanisms. Two types of virus-related small interfering RNAs (siRNAs) were detected: siRNAs of 21 to 22 nucleotides (nt) in size that are related to the coding regions (Rep, TrAP, REn, and movement protein genes) and a 24-nt population primarily associated to the intergenic regions. Methylation levels of the PepGMV A intergenic and coat protein (CP) coding region were measured by a bisulfite sequencing approach. An inverse correlation was observed between the methylation status of the intergenic region and the concentration of viral DNA and symptom severity. The intergenic region also showed a methylation profile conserved in all times analyzed. The CP region, on the other hand, did not show a defined profile, and its methylation density was significantly lower than the one found on the intergenic region. The participation of both PTGS and TGS mechanisms in host recovery is discussed.

APA, Harvard, Vancouver, ISO, and other styles

10

Schröder, Jens A., and Pauline E. Jullien. "The Diversity of Plant Small RNAs Silencing Mechanisms." CHIMIA International Journal for Chemistry 73, no. 5 (May 29, 2019): 362–67. http://dx.doi.org/10.2533/chimia.2019.362.

Full text

Abstract:

Small RNAs gene regulation was first discovered about 20 years ago. It represents a conserve gene regulation mechanism across eukaryotes and is associated to key regulatory processes. In plants, small RNAs tightly regulate development, but also maintain genome stability and protect the plant against pathogens. Small RNA gene regulation in plants can be divided in two canonical pathways: Post-transcriptional Gene Silencing (PTGS) that results in transcript degradation and/or translational inhibition or Transcriptional Gene Silencing (TGS) that results in DNA methylation. In this review, we will focus on the model plant Arabidopsis thaliana. We will provide a brief overview of the molecular mechanisms involved in canonical small RNA pathways as well as introducing more atypical pathways recently discovered.

APA, Harvard, Vancouver, ISO, and other styles

More sources

Dissertations / Theses on the topic "Plant gene silencing"

1

McMaster, S. "Studies on Gene Silencing in Plant Parasite Nematodes." Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.501370.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

George, Gavin M. (Gavin Mager). "Virus induced gene silencing for the study of starch metabolism." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4024.

Full text

Abstract:

Thesis (PhD (Plant Biotechnology))--University of Stellenbosch, 2010.
ENGLISH ABSTRACT: Virus Induced Gene Silencing (VIGS) was optimized to allow for the study of starch metabolism. The plastidial inorganic pyrophosphatase gene, for which a mutant has never been identified, was studied using VIGS and it was found to have a broad role in this subcellular compartment. The accumulation of inorganic pyrophosphate limited the production of starch, carotenoids, chlorophyll, and increased the plants susceptibility to drought stress. These effects highlight the importance of this enzyme in maintaining a low intraplastidial concentration of PPi providing an environment which facilitates these anabolic processes. Several genes involved in starch synthesis and degradation were also targeted with the aim of establishing a system of multiple gene silencing for the study of metabolic pathways. One, two and three genes were successfully silenced using this system which was validated based on previously published data. Interestingly, simultaneous silencing of the two isoforms of disproportionating enzyme led to a novel phenotype as a large reduction in starch instead of the expected increase was observed.
No Afrikaans abstract available

APA, Harvard, Vancouver, ISO, and other styles

3

Dalzell, J. J. "The development of gene silencing strategies for plant parasitic nematodes." Thesis, Queen's University Belfast, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.546037.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Chau, Ling Bess. "Capacity of plant-derived siRNA for gene silencing in mammalian cells." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B36778874.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Chau, Ling Bess, and 周玲. "Capacity of plant-derived siRNA for gene silencing in mammalian cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B36778874.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Davies, Gareth John. "Co-suppression of chalcone synthase genes in Arabidopsis thaliana." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318010.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Raponi, Mitch Biochemistry &amp Molecular Genetics UNSW. "Antisense RNA-mediated gene silencing in fission yeast." Awarded by:University of New South Wales. Biochemistry and Molecular Genetics, 2001. http://handle.unsw.edu.au/1959.4/18277.

Full text

Abstract:

The major aims of this thesis were to investigate the influence of i) antisense gene location relative to the target gene locus (?????location effect?????), ii) double-stranded RNA (dsRNA) formation, and iii) over-expression of host-encoded proteins on antisense RNA-mediated gene regulation. To test the location effect hypothesis, strains were generated which contained the target lacZ gene at a fixed location and the antisense lacZ gene at various genomic locations including all arms of the three fission yeast chomosomes and in close proximity to the target gene locus. A long inverse-PCR protocol was developed to rapidly identify the precise site of antisense gene integration in the fission yeast transformants. No significant difference in lacZ suppression was observed when the antisense gene was integrated in close proximity to the target gene locus, compared with other genomic locations, indicating that target and antisense gene co-localisation is not a critical factor for efficient antisense RNA-mediated gene suppression in vivo. Instead, increased lacZ down-regulation correlated with an increase in the steady-state level of antisense RNA, which was dependent on genomic position effects and transgene copy number. In contrast, convergent transcription of an overlapping antisense lacZ gene was found to be very effective at inhibiting lacZ gene expression. DsRNA was also found to be a central component of antisense RNA-mediated gene silencing in fission yeast. It was shown that gene suppression could be enhanced by increasing the intracellular concentration of non-coding lacZ RNA, while expression of a lacZ panhandle RNA also inhibited beta-galactosidase activity. In addition, over-expression of the ATP-dependent RNA-helicase, ded1, was found to specifically enhance antisense RNA-mediated gene silencing. Through a unique overexpression screen, four novel factors were identified which specifically enhanced antisense RNA-mediated gene silencing by up to an additional 50%. The products of these antisense enhancing sequences (aes factors), all have natural associations with nucleic acids which is consistent with other proteins which have previously been identified to be involved in posttranscriptional gene silencing.

APA, Harvard, Vancouver, ISO, and other styles

8

Starkus, Laura. "Virus-induced gene silencing of putative Diuraphis noxia (Kurdjumov) resistance genes in wheat." Thesis, Manhattan, Kan. : Kansas State University, 2010. http://hdl.handle.net/2097/4193.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Gammelgård, Elin. "Interactions of potato virus A with host plants : recombination, gene silencing and non-hypersensitive resistance /." Uppsala : Dept. of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/2007111.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Buchmann, Cody. "Reversal of RNA-mediated gene silencing pathways by geminivirus AL2 and L2 proteins." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1221847080.

Full text

APA, Harvard, Vancouver, ISO, and other styles

More sources

Books on the topic "Plant gene silencing"

1

Matzke, M. A., and A. J. M. Matzke, eds. Plant Gene Silencing. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Mysore, Kirankumar S., and Muthappa Senthil-Kumar, eds. Plant Gene Silencing. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2453-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Dalmay, T., ed. Plant gene silencing: mechanisms and applications. Wallingford: CABI, 2017. http://dx.doi.org/10.1079/9781780647678.0000.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Erdmann, V. A., and Jan Barciszewski. Non coding RNAs in plants. Heidelberg: Springer, 2011.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

5

Non coding RNAs in plants. Heidelberg: Springer, 2011.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

6

Courdavault, Vincent, and Sébastien Besseau, eds. Virus-Induced Gene Silencing in Plants. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0751-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Paszkowski, Jerzy, ed. Homologous Recombination and Gene Silencing in Plants. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1094-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Meyer, Peter, ed. Gene Silencing in Higher Plants and Related Phenomena in Other Eukaryotes. Berlin, Heidelberg: Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/978-3-642-79145-1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

(Editor), M. A. Matzke, and A.J.M. Matzke (Editor), eds. Plant Gene Silencing. Springer, 2000.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

10

Matzke, M. A., and A. J. M. Matzke. Plant Gene Silencing. M a Matzke, 2012.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

More sources

Book chapters on the topic "Plant gene silencing"

1

Mandahar, Chuni L. "Gene Silencing." In Molecular Biology of Plant Viruses, 255–69. Boston, MA: Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-5063-1_13.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Finnegan, E. J., and K. A. Kovac. "Plant DNA methyltransferases." In Plant Gene Silencing, 69–81. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3_5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Chandler, Vicki L., William B. Eggleston, and Jane E. Dorweiler. "Paramutation in maize." In Plant Gene Silencing, 1–25. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3_1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Meins, Frederick. "RNA degradation and models for post-transcriptional gene silencing." In Plant Gene Silencing, 141–53. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3_10.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Morel, Jean-Benoit, and Hervé Vaucheret. "Post-transcriptional gene silencing mutants." In Plant Gene Silencing, 155–64. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3_11.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Fagard, Mathilde, and Hervé Vaucheret. "Systemic silencing signal(s)." In Plant Gene Silencing, 165–73. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3_12.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Marathe, Rajendra, Radhamani Anandalakshmi, Trent H. Smith, Gail J. Pruss, and Vicki B. Vance. "RNA viruses as inducers, suppressors and targets of post-transcriptional gene silencing." In Plant Gene Silencing, 175–86. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3_13.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Covey, Simon N., and Nadia S. Al-Kaff. "Plant DNA viruses and gene silencing." In Plant Gene Silencing, 187–202. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3_14.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Iyer, Lakshminarayan M., Siva P. Kumpatla, Mahesh B. Chandrasekharan, and Timothy C. Hall. "Transgene silencing in monocots." In Plant Gene Silencing, 203–26. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3_15.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

De Wilde, Chris, Helena Van Houdt, Sylvie De Buck, Geert Angenon, Geert De Jaeger, and Ann Depicker. "Plants as bioreactors for protein production: avoiding the problem of transgene silencing." In Plant Gene Silencing, 227–39. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4183-3_16.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Plant gene silencing"

1

"Efficient eradication of potato viruses by induction of posttranscriptional gene silencing in transgenic potato." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-009.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Khan, Sher Afzal. "Gene silencing of herbivorous insects by host plant chloroplast genome modification." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94925.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Shrub, K. V., A. V. Kolubako, and Ye A. Nikolaichik. "The receptor-like kinase RLK4 from Solanaceae family plants contributes to immune response." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.226.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

"Development of a new method for eradication of viruses by induction of posttranscriptional gene silencing in transgenic potato plants." In Current Challenges in Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk State University, 2019. http://dx.doi.org/10.18699/icg-plantgen2019-46.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Suprunova, T. P., N. V. Markin, A. N. Ignatov, A. G. Solovyov, N. O. Kalinina, and M. E. Talyansky. "Use of dsRNA-based antiviral compounds to protect potato plants." In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-132.

Full text

Abstract:

One of the most important food crops in the world, the potato (Solanum tuberosum L.) is infected with many viruses, of which the y virus (Potato virus Y, PVY) is the most important economically, causing significant crop losses. Several alternative methods of dsRNA delivery have been tested, with the most promising being spray - induced gene silencing (SIGS). The results showed a high effect of preventive use of dsRNA. Treatment with the initial working concentration of dsRNA protected 100% and 65% of plants from virus propagation for 14 and 21 days, respectively, and 65% of plants were protected by the minimum tested concentration (10 ng/MCL) for 14 days. Therapeutic use of dsRNA 3 days after inoculation did not significantly affect the dynamics of virus accumulation in the plant. Thus, in the course of the experiment, a high biological antiviral effectiveness of dsRNA was demonstrated in the preventive treatment of potato plants against the background of artificial infection of plants with the PVY virus.

APA, Harvard, Vancouver, ISO, and other styles

6

Santos, Taís Araújo, Elza Thaynara Cardoso De Menezes Assis, Jocilene Dos Santos Pereira, and Letícia Maróstica De Vasconcelos. "A TECNOLOGIA CRISPR/CAS9 NA RESISTÊNCIA DE PLANTAS CONTRA PATÓGENOS FÚNGICOS." In I Congresso de Engenharia de Biotecnologia. Revista Multidisciplinar de Educação e Meio Ambiente, 2021. http://dx.doi.org/10.51189/rema/1372.

Full text

Abstract:

Introdução: As plantas são suscetíveis a um grande número de patógenos, incluindo os fungos. Fitopatógenos fúngicos são responsáveis por inúmeras doenças, como ferrugem, oídio, podridão, entre outras. Diferentes estratégias têm sido desenvolvidas para aumentar a resistência fúngica em espécies de plantas com base no conhecimento atual dos mecanismos moleculares envolvidos na interação planta-patógeno. As tecnologias de edição de genoma progrediram rapidamente e se tornaram as ferramentas genéticas mais utilizadas para o melhoramento de plantas. Entre essas, temos a aplicação do sistema formado por repetições palindrômicas curtas, interespaçadas e regularmente agrupadas (CRISPR), e sua proteína associada-9 (Cas9). Objetivo: Apresentar a tecnologia de edição de genoma CRISPR/Cas9 com foco na sua aplicação para o aumento da resistência de plantas á patógenos fúngicos. Metodologia: A pesquisa foi realizada nas bases de dados: PubMed e Scopus. Para alcançar o máximo de precisão na estratégia de busca, utilizou-se os descritores: “plant”, “pathogen”, “fungi or fungus”, “CRISPR”. Resultados: A maioria dos trabalhos envolvendo a resistência de plantas contra patógenos fúngicos estavam relacionados como a capacidade do sistema CRISPR/Cas9 em induzir mutagênese direcionada, com competência em silenciar genes implicados na interação planta-fungo. Foi possível observar vários estudos onde os genes de suscetibilidade da planta hospedeira foram inativados, pois eram necessários para o ciclo de vida do patógeno, demonstrando que a tecnologia é aplicável à resistência a doenças fúngicas em plantas, pois o silenciamento de um determinado gene na planta pode resultar em uma suscetibilidade no fungo. Conclusão: Os resultados demonstram a aplicação vantajosa do sistema CRISPR/Cas9 para o melhoramento de culturas no que diz respeito à resistência a patógenos. O aumento dessa resistência possui um papel importante, pois os fungos fitopatogênicos representam uma ameaça para a produção e o rendimento das safras agrícolas.

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!