To see the other types of publications on this topic, follow the link: Plant-extracted antioxidants.
Journal articles on the topic 'Plant-extracted antioxidants'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Plant-extracted antioxidants.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Moroșan, Elena, Magdalena Mititelu, Doina Drăgănescu, Dumitru Lupuliasa, Emma Adriana Ozon, Oana Karampelas, Cerasela Elena Gîrd, et al. "Investigation into the Antioxidant Activity of Standardized Plant Extracts with Pharmaceutical Potential." Applied Sciences 11, no. 18 (September 17, 2021): 8685. http://dx.doi.org/10.3390/app11188685.
Full textAbstract:
Given the important role of antioxidants in the cellular degeneration process, as well as the increased interest in recent years related to the use of natural antioxidants in therapy, the present study aims to investigate the antioxidant activity of a new pharmaceutical product containing natural antioxidants extracted from plant raw materials. In a first step, the product conventionally named “CILTAG”, containing a mixture in equal proportions of 10% hydroalcoholic extractive solutions of dried plant raw materials from Medicaginis herba, Trifolii pratense flores, Ginkgo bilobae folium, Myrtilli fructus, and Cynosbati fructus, was obtained. In the second stage, the antioxidant activity of the hydroalcoholic extractive solutions included in the pharmaceutical product was tested by chemiluminescence and electrochemical methods and by the superoxide dismutase (SOD) method. The electrochemical determination of the antioxidant capacity of hydroalcoholic extractive solutions was based on a method that provides an indirect evaluation of the presumed antioxidant properties of some compounds using Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), as a standard measurement. The experimental results indicate a significant antioxidant potential for both the analyzed plant extractive solutions that are part of CILTAG and the final product.
2
Koubaa, Mohamed, Ameni Ktata, Francisco J. Barba, Nabil Grimi, Houcine Mhemdi, Fatma Bouaziz, Dorra Driss, and Semia Ellouz Chaabouni. "Water-soluble polysaccharides from Opuntia stricta Haw. fruit peels: recovery, identification and evaluation of their antioxidant activities." International Agrophysics 29, no. 3 (July 1, 2015): 299–306. http://dx.doi.org/10.1515/intag-2015-0035.
Full textAbstract:
Abstract Opuntia stricta Haw. is considered as one of the most common cactus plant growing in Tunisia. Extracting valuable compounds from its fruit peel, considered as by-product, is drawing more and more attention, making it on the verge of commercialization. Water-soluble polysaccharides were extracted from Opuntia stricta Haw. peels, and their chemical composition assessed using thin layer chromatography. The antioxidant activities of the extracted polysaccharides were assessed using 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, total antioxidant activity and reducing power capacity. The extraction yield of water-soluble polysaccharides was 7.53±0.86%. The chemical composition revealed the presence of rhamnose, arabinose, glucose, mannose, galactose and galacturonic acid. The infra-red spectroscopic analysis showed a similar structure to that of Opuntia ficus-indica polysaccharide peels. Additionally, the extracted polysaccharides exhibited high antioxidant activities. In fact, the free radical scavenging activity (half inhibition concentration = 6.5 mg ml−1 with 94.9% inhibition at 50 mg ml−1), the total antioxidant activity (100 μg ascorbic acid equivalent at 50 mg polysaccharides) and the reducing power capacity (absorbance 700 nm = 0.7 at 50 mg ml−1), appeared to be interesting compared to natural and synthetic antioxidants. Therefore, water-soluble polysaccharides from Opuntia stricta Haw. fruit peels could be a natural alternative to replace synthetic antioxidants.
3
Fadda, Angela, Daniele Sanna, El Hassan Sakar, Said Gharby, Maurizio Mulas, Silvia Medda, Nese Sahin Yesilcubuk, et al. "Innovative and Sustainable Technologies to Enhance the Oxidative Stability of Vegetable Oils." Sustainability 14, no. 2 (January 12, 2022): 849. http://dx.doi.org/10.3390/su14020849.
Full textAbstract:
To meet consumers’ demand for natural foods, edible oil producers and food processing industries are searching for alternatives to synthetic antioxidants to protect oils against oxidation. Antioxidant compounds extracted from different plant parts (e.g., flowers, leaves, roots, and seeds) or sourced from agri-food industries, including residues left after food processing, attract consumers for their health properties and natural origins. This review, starting from a literature research analysis, highlights the role of natural antioxidants in the protection of edible oils against oxidation, with an emphasis on the emerging and sustainable strategies to preserve oils against oxidative damage. Sustainability and health are the main concerns of food processing industries. In this context, the aim of this review is to highlight the emerging strategies for the enrichment of edible oils with biomolecules or extracts recovered from plant sources. The use of extracts obtained from vegetable wastes and by-products and the blending with oils extracted from various oil-bearing seeds is also pointed out as a sustainable approach. The safety concerns linked to the use of natural antioxidants for human health are also discussed. This review, using a multidisciplinary approach, provides an updated overview of the chemical, technological, sustainability, and safety aspects linked to oil protection.
4
Lourenço, Sofia C., Margarida Moldão-Martins, and Vítor D. Alves. "Antioxidants of Natural Plant Origins: From Sources to Food Industry Applications." Molecules 24, no. 22 (November 15, 2019): 4132. http://dx.doi.org/10.3390/molecules24224132.
Full textAbstract:
In recent years, great interest has been focused on using natural antioxidants in food products, due to studies indicating possible adverse effects that may be related to the consumption of synthetic antioxidants. A variety of plant materials are known to be natural sources of antioxidants, such as herbs, spices, seeds, fruits and vegetables. The interest in these natural components is not only due to their biological value, but also to their economic impact, as most of them may be extracted from food by-products and under-exploited plant species. This article provides an overview of current knowledge on natural antioxidants: their sources, extraction methods and stabilization processes. In addition, recent studies on their applications in the food industry are also addressed; namely, as preservatives in different food products and in active films for packaging purposes and edible coatings.
5
Mosca, Federica, Gádor Hidalgo, Juliana Villasante, and María Almajano. "Continuous or Batch Solid-Liquid Extraction of Antioxidant Compounds from Seeds of Sterculia apetala Plant and Kinetic Release Study." Molecules 23, no. 7 (July 18, 2018): 1759. http://dx.doi.org/10.3390/molecules23071759.
Full textAbstract:
This work has been intended to investigate the antioxidant properties of compounds extracted from seeds of Sterculia apetala (a plant from Central America) in order to add further results to the relatively poor existing literature on the beneficial properties of this plant. Different extraction methodologies were used such as batch or continuous extraction conditions using water or ethanol 50% as solvents. The kinetic study has allowed estimation of the effective diffusion coefficients in a continuous solid-liquid extraction, highlighting the strict dependence of the diffusion rate and temperature and kind of solvent, showing the highest diffusion rate with ethanol 50% at 60 °C. The comparison between different techniques and two solvents led to the selection of water as the best extraction solvent while batch mechanically-agitated extraction was the most efficient mode, with the benefits of use of an environmental-friendly solvent and reduction in process time to achieve a high amount of extracted phenolic compounds. The analysis techniques used were ABTS and Folin-Ciocalteau methods to investigate the antioxidant activity and quantify the Total Phenolic Content (TPC) respectively. Also, different fatty acids were extracted from Sterculia apetala seeds and analysed by Gas Chromatography in order to quantify other interesting chemical species besides antioxidants.
6
Almanea, Abdulaziz, Gamal S. Abd El-Aziz, and Mohamed Morsi M. Ahmed. "The Potential Gastrointestinal Health Benefits of Thymus Vulgaris Essential Oil: A Review." Biomedical and Pharmacology Journal 12, no. 04 (December 24, 2019): 1793–99. http://dx.doi.org/10.13005/bpj/1810.
Full textAbstract:
In recent times, medicinal plants have received great attention worldwide due to their effective pharmacological properties and therapeutic benefits. Numerous chemical compounds extracted from various medicinal plants have manifold biological activities. Thymus vulgaris (TV) is a flowering plant with an aromatic odor that has been broadly applied in conventional medicine, food additives, and phyto-pharmaceutical preparations. It is recognized to have promising therapeutic potential for curing various types of diseases. The essential oil extracted from TV, which contains a high quantity of flavonoids, possess antioxidant and antimicrobial activities. Consequently, it could be utilized as a good source in developing novel natural antioxidants and antibiotics. This review explores some of the potential health benefits of TV essential oil (TVEO) on the gastrointestinal tract.
7
Atun, Sri, Sri Handayani, Anna Rakhmawati, Nur Aini Purnamaningsih, Bian Ihda An Naila, and Astuti Lestari. "Study of Potential Phenolic Compounds from Stems of Dendrophthoe Falcata (Loranthaceae) Plant as Antioxidant and Antimicrobial Agents." Oriental Journal of Chemistry 34, no. 5 (October 18, 2018): 2342–49. http://dx.doi.org/10.13005/ojc/340515.
Full textAbstract:
Dendrophthoe falcata is a hemiparasitic plant that has been used in traditional medicine.The study was conducted to identify the total phenolic and flavonoid compounds, to test antioxidant, and antimicrobial activity of ethanol extract stems of D. falcata parasite on Melia azedarach host tree. The dry powder stems of D. falcata was extracted with ethanol. The ethanol extract was subsequently partitioned successively using n-hexane, chloroform, and ethyl acetate. Each fraction was analyzed by a quantitative phenolic and flavonoid content with spectrophotometer method, and tested as antioxidant and antimicrobial activites. Antioxidant activity was performed by 2,2-diphenyl-1-picrilhidrazil (DPPH) method, while antimicrobial assay used pathogenic bacteria by disk-diffusion method. The results concluded that the stem of D. falcata plant showed a high content of phenolic and flavonoid compounds, very high antioxidant and moderate antimicrobial activities. It was also found that stems of D. falcata contain potential phenolic compounds that can be used as natural antioxidants and the treatment of various infections caused by microbes.
8
Dirgantara, Septriyanto, As’ari Nawawi, and Muhamad Insanu. "Uji Aktivitas Antioksidan Tiga Spesies Tanaman Sarang Semut (Famili: Rubiaceae) Asal Kabupaten Merauke, Papua." JURNAL BIOLOGI PAPUA 5, no. 1 (October 13, 2018): 10–14. http://dx.doi.org/10.31957/jbp.517.
Full textAbstract:
Sarang semut plant is one of indigenous medicinal plants of Merauke region, the Papua province. Local people use the bulb of sarang semut plants to cure inflammation, muscle pain and enhancing immunity. However rare research of the plants has been done especially on the antioxidant activity. The objective of this research was to investigate the antioxidant activity of three species of sarang semut plants which were Myrmecodia beccarii, Myrmecodia sp. and Hydnophytum sp. The test was necessary to identify the inhibitory concentration level of plant extract in blocking a free radical (IC50). Parts of plants were extracted with maceration using methanol for 3 x 24 h to produce concentrated methanol. Plant extracts of three species were then subject to antioxidant activity test based on free radical blocking of 1,1-Difenil-2-Pikrilhidrazil (DPPH) qualitatively and quantitatively using Ultraviolet-Visible spectrophotometry. The results showed the methanol extract of M. beccarii, Myrmecodia sp. and Hydnophytum sp. were active as antioxidants with IC50 from 8.18 ppm, 21.79 ppm, 25.31 ppm, respectively while Vitamin C as a control has IC50 7.85 ppm. In conclusion, three plant species of sarangsemut are potential as natural antioxidant and plant extracts of M. beccarii showed the highest antioxidant activity among others. Key words: Antioxidant, sarang semut plants, Rubiaceae, DPPH.
9
Nutan, ,., Manas Kr Das, Gaurav Saxena, and Nitin Kumar. "To perform phytochemical screening and study the antioxidant potential of isolated compound from Hemidesmus indicus." Journal of Drug Delivery and Therapeutics 9, no. 2 (March 15, 2019): 188–91. http://dx.doi.org/10.22270/jddt.v9i2.2401.
Full textAbstract:
“Anantmul” is an important and widely used medicinal plant. The study aimed to determine the physicochemical composition, bioactive compounds and antioxidant activity of Hemidesmus indicus (Asclepiadaceae). The roots of the plant were collected and sequentially extracted using petroleum ether, ethyl acetate, and methanol. The preliminary phytochemical screening of extracts was carried out and found to be a good source of bioactive compounds. Biological activities of flavonoid and phenolic compounds have been discovered in several latest studies. Further phytochemical isolation was carried out, and Lupeol was isolated. Phenols are shown to be multifunctional antioxidants which will perform as singlet oxygen quenchers. Lupeol was evaluated for in-vitro antioxidant activity. It showed a correlation with antioxidant activity by DPPH (IC50 = 0.52, P 6 0.05) and H2O2 (IC50 = 0.43, P 6 0.05). The results show promising perspectives for the exploitation and use of anantmul rhizome as a constituent of anti-aging as well as anticancer diet.
Keywords: Anantmul, Lupeol, Antioxidant, DPPH
10
Osman Adam, Osman Adam, Ragaa Satti Mohmmed Abadi, and Saad Mohamed Hussein Ayoub. "Antioxidant activity, Total Phenolic and Flavonoid Contents and Cytotoxic activity of Euphorbia aegyptiaca." Journal of Drug Delivery and Therapeutics 10, no. 2 (March 15, 2020): 37–41. http://dx.doi.org/10.22270/jddt.v10i2.3911.
Full textAbstract:
Euphorbia aegyptiaca is a herbaceous plant traditionally used in Sudan for treatment of various diseases, and the study of this plant is still limited. The aim of the present study was to screen the phytochemicals and to assess the Antioxidant activity, total phenolic, flavonoid contents and cytotoxic activity of Euphorbia aegyptiaca. The plant material was extracted successively by Soxhlet apparatus using n-hexane, chloroform and methanol. The chemical constituents of the extracts were carried out using the standard procedures. The Folin- Ciocalteu and Aluminium chloride method was employed to calculate the total phenolic and flavonoid content, respectively. The antioxidant activity, was assessed by measuring the scavenging activity of the DPPH (2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl) and Propyl Gallate as standard antioxidants. While cytotoxic activities were screened using brine shrimp. Phytochemical screening studies revealed that flavonoids, tannins, coumarins, saponins, sterols, terpenes, anthraquinones and alkaloids were the main phytochemicals present in extracts of E. aegyptiaca. The methanol extract showed the highest level of total phenolic contents (173.49±2.427 mg GAE/g) and flavonoid content (239.53±7.90 mg QE/g), and the highest antioxidant activity 89% with least (IC50 0.0449µg/ml), and the no toxicity against brine shrimp (LD50 3423.156). Furthermore, no toxicity in all extracts was observed. The present study is the first evaluation regarding the characterization of E. aegyptiaca and its safety, and the results demonstrate its antioxidant potential and suggest its safe therapeutic use. The results suggest that methanol extract is a rich source of phytochemicals and exhibits highest amount of and total phenolic, flavonoid content and significant antioxidant activity and it has no cytotoxic activity. E. aegyptiaca plant can be regarded as a promising Source of naturally occurring potential antioxidants.
Keywords: Euphorbia aegyptiaca, Sudan, Antioxidant, Total phenolic, total flavonoid, Cytotoxicity.
11
Muzykiewicz, Anna, Anna Nowak, Joanna Zielonka-Brzezicka, Katarzyna Florkowska, Wiktoria Duchnik, and Adam Klimowicz. "Comparison of antioxidant activity of extracts of hop leaves harvested in different years." Herba Polonica 65, no. 3 (September 1, 2019): 1–9. http://dx.doi.org/10.2478/hepo-2019-0013.
Full textAbstract:
Summary Introduction: Hop (Humulus lupulus L.) is a common plant in Europe, with many beneficial health effects. In addition to the use in brewing, hops are a valuable source of active substances used in conventional and folk medicine, such as humulones and lupulones, as well as antioxidants, including phenolic compounds. Objective: The aim of the study was to evaluate and compare the antioxidant activity of alcoholic extracts of fresh hop leaves collected in 2017 and 2018. Material and methods: The raw material consisting of fresh hop leaves was extracted using ultrasound-assisted extraction. Methyl, ethyl and isopropyl alcohol at three concentrations were used as extractants. The antioxidant activity of extracts was determined using DPPH and FRAP methods. Total phenolic content was evaluated using the Folin-Ciocalteu technique. Results: All the extracts showed antioxidant potential as well as the phenolic content. Regardless of the harvesting year and methods of evaluation, the highest antioxidant activity and the total polyphenol content were observed for extracts prepared in undiluted methanol, obtained during one hour lasting extraction. Conclusion: The results of the studies have suggested that hop leaves can be a potential source of health-promoting antioxidants.
12
Ureta, Romnick, Siegfred Mejico, and Yvonne Maranan. "Free Radical Scavenging Activity and Antioxidants of Hydrocotyle vulgaris L. (Pennywort): Baseline Study in Developing Biocosmetic-Antidote for Pathological Aging." International Journal of Pharmacology, Phytochemistry and Ethnomedicine 10 (July 2018): 1–12. http://dx.doi.org/10.18052/www.scipress.com/ijppe.10.1.
Full textAbstract:
Pathological aging due to harmful free radicals and oxidative stress has been a serious threat to human health (Kensella & He, 2008). Thus, the study aimed to evaluate the free radical scavenging activity, antioxidants, essential vitamins, and physicochemical properties of Hydrocotyle vulgaris L. (Pennywort) which could serve as basis in developing a biocosmetic antidote for pathological aging. H.vulgaris L. plant was harvested, air dried and then extracted via soxhlet. UV-vis spectroscopy was used for scavenging activity and antioxidants of the plant while High Performance Liquid Chromatography (HPLC) was applied for the identification of essential vitamins. Phytochemicals, pH and spreadability values of the plant were also tested. Results revealed that H.vulgaris L. has an abundant presence of flavonoid and alkaloid while traced presence of tannin. Good trend for scavenging activity was also observed exhibiting low absorbance of possible free radicals with IC50 of 29.75 and antioxidant activity of 158.13 (Total Phenolics as gallic acid in %w/w). Meanwhile, β-carotene (10.4 mg/kg), Riboflavin (4.08mg/kg), and Vitamins C (70.2mg/kg) and E (26.9mg/kg) were also found in the plant. Non-irritating pH levels of 5.7 and 7.14 for 50% and 75% concentrations of the extracts with good spreadability value of 17.51 g∙cm/sec (for 75% concentration) were also obtained. Based on the findings of the study, Pennywort has a very promising therapeutic characteristics with its good scavenging activity and antioxidants. Thus, the development of a biocosmetic product that could serve as an antidote for pathological aging from Pennywort appeared to be very apparent.
13
Mahmood, Riaz, Sameera Parveen, Ramesh Ck, and Pallavi M. "FOLKLORE MEDICINAL ORCHIDS FROM SOUTH INDIA: THE POTENTIAL SOURCE OF ANTIOXIDANTS." Asian Journal of Pharmaceutical and Clinical Research 11, no. 6 (June 7, 2018): 194. http://dx.doi.org/10.22159/ajpcr.2018.v11i6.24726.
Full textAbstract:
Objective: Orchids are widely used the economically important ornamental plant. Conventionally, they were also used for the treatment of several diseases. In the present study, five species of medicinal Orchids from South India were selected to evaluate their antioxidant potential.Methods: The selected species were extracted by Soxhlet method using 70% ethanol. The extracts obtained were analyzed for various quantitative and antioxidant assays followed by correlation analysis in between quantitative and antioxidant activity.Results: Antioxidant data revealed that among the extracts of five orchids, Coelogyne breviscapa was proved to be superior in terms of antioxidant activities, followed by Aerides maculosum, Dendrobium macrostachyum, Pholidota pallida, and Vanda testacea. Correlation analysis was performed, and the results proved simple positive correlation and highest average value of “r” (correlation coefficient) for antioxidant activities with quantitative were the total antioxidants, total phenolics, total flavonoids, and ascorbic acid content. Among the qualitative antioxidant activities, the highest average value of “r” was shown by 2, 2-diphenyl-1-picrylhydrazyl, iron chelating, 2,2-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid, and superoxide radical.Conclusion: The study documents that orchid plants have significant antioxidant potential which can contribute to human health.
14
Kamarul Zaman, Munirah Adibah, Azzreena Mohamad Azzeme, Siti Nurhafizah Ramli, Noor Azmi Shaharuddin, Syahida Ahmad, and Siti Nor Akmar Abdullah. "Solvent extraction and its effect on phytochemical yield and antioxidant capacity of woody medicinal plant, Polyalthia bullata." BioResources 15, no. 4 (October 29, 2020): 9555–68. http://dx.doi.org/10.15376/biores.15.4.9555-9568.
Full textAbstract:
Polyalthia bullata is a woody medicinal plant that contains antioxidant compounds. Finding a suitable solvent is important to obtain a high yield of antioxidants in the phenolic, flavonoid, and terpenoid families. In this study, from different solvent extracts, the leaf methanolic extract exhibited the highest total phenolic content (TPC), total flavonoid content (TFC), total terpenoid content (TTC), and total antioxidant activity. For woody parts of stem and roots, methanol was the best solvent for all phytochemicals except for phenolics, which accumulated in the roots and were extracted more efficiently using ethanol. However, the methanolic extracts from both tissues displayed the best antioxidant capacity. Gas chromatography-mass spectrometry (GC-MS) profiling data showed the presence of antioxidant compounds such as thymol, phytol, and neophytadiene in the leaf; trans-farnesol, n-hexadecanoic acid, and 9-Octadecenamide in the stem; and fatty acid (cis-vaccenic) and its methyl ester (11-Octadecanoic acid, methyl ester and [1,1’-bicyclopropyl]-2-octanoic acid, 2’-hexyl-methyl ester) in the roots. These findings reveal important compounds that are present in different plant parts of P. bullata.
15
Nastiti, Kunti, Noval Noval, and Darini Kurniawati. "Uji Aktivitas Antioksidan Kombinasi Infusa Daun Sirih (Piper betle L), Ekstrak Etanolik Tanaman Bundung (Actinuscirpus grossus) dan Kulit Jeruk Nipis (Citrus aurantifolia)." Jurnal Surya Medika 7, no. 1 (August 30, 2021): 115–22. http://dx.doi.org/10.33084/jsm.v7i1.2647.
Full textAbstract:
Piper betle L., the leaf, is the most often used for treatment and has been scientifically proven to have antibacterial activity. Actinuscirpus grossus plants are commonly found in Kalimantan and have antimicrobial properties. Citrus aurantifolia peel has antioxidant effects and nutritious chemical compounds in medicine. The purpose of this study was to determine the effect of antioxidant activity in a combination of Piper betle L., leaf infusion, ethanolic extract of Actinuscirpus grossus plant, and Citrus aurantifolia peel. The research method was experimental by making Piper betle L. leaves infused with water solvent. Simplisia powder of Actinuscirpus grossus plants and Citrus aurantifolia peel extracted by maceration with 95% ethanol solvent. The combination of the three plants was then tested for antioxidants by the DPPH method. Quercetin is used as a positive control. The results showed that the combination of the three plants had moderate antioxidant activity (IC50 128 ppm), but the antioxidant activity was smaller than quercetin (IC50 16.88 ppm). The combination of Piper betle L., leaf infusion, ethanolic extract of Actinuscirpus grossus plant, and Citrus aurantifolia peel has a moderate antioxidant category.
16
Bajpai, Monika, Madhusudan S, and Sibi G. "Standardized method to extract phenolic compounds from Lagerstroemia speciosa L. (Jarul) for enhanced antioxidant activity." Journal of Applied and Natural Science 13, no. 3 (September 15, 2021): 1041–47. http://dx.doi.org/10.31018/jans.v13i3.2870.
Full textAbstract:
Phenolic compounds contribute to the antioxidant property of plants and the efficient extraction of plant phenolics could enhance its antioxidant potential. Lagerstroemia speciosa (L.) Pers. has been investigated for its pharmacological activities, but comparing its antioxidant activities of phenolics derived from its various parts has a key role in developing natural antioxidants. This study was conducted to standardize the extraction of phenolics from leaves, pods and branches of L. speciosa plant, followed by determination of antioxidant activities of their solvent fractions. Phenolic compounds were extracted from the leaves, pods and branches under different parameters such as temperature, pH, type of solvent and volume of the solvent. The extracted phenolic compounds were subjected to solvent fractions and antioxidant assays were performed. Among the various extraction methods tested, the best method was 50% ethanol +1% HCl, refluxing temperature, 100 ml of solvent and 1 hour extraction time. The best solvent fractions were determined as NaHCO3 + ethyl acetate for pods and ethyl acetate alone for leaves and branches with extracted phenolics content of 150.2 mg/g, 136.2 mg/g and 82.9 mg/g, respectively. The chloroform fraction was best among the fractions with maximum ascorbic acid equivalent (ASE) in all the parts of L. speciosa tested. Ferrous ion chelating capacity indicated that butanol fraction had the highest chelation and the same was recorded in ferric ion chelating assay with an EC50 value of 28.2. Hydroxyl radical scavenging activity of the fractions indicated that NaHCO3 + EtOAc fraction of pods had potential activity. Thus, the phenolic compounds from L. speciosa are excellent sources for future investigation on potent natural antioxidant compounds.
17
Huang, Xiuqiong, Wei Gao, Xuan Yun, Zhixing Qing, and Jianguo Zeng. "Effect of Natural Antioxidants from Marigolds (Tagetes erecta L.) on the Oxidative Stability of Soybean Oil." Molecules 27, no. 9 (April 30, 2022): 2865. http://dx.doi.org/10.3390/molecules27092865.
Full textAbstract:
In recent years, synthetic antioxidants that are widely used in foods have been shown to cause detrimental health effects, and there has been growing interest in antioxidants realised from natural plant extracts. In this study, we investigate the potential effects of natural antioxidant components extracted from the forage plant marigold on the oxidative stability of soybean oil. First, HPLC-Q-TOF-MS/MS was used with 1,1-diphenyl-2-picrylhydrazyl (DPPH) to screen and identify potential antioxidant components in marigold. Four main antioxidant components were identified, including quercetagetin-7-O-glucoside (1), quercetagetin (2), quercetin (3) and patuletin (4). Among them, quercetagetin (QG) exhibited the highest content and the strongest DPPH radical scavenging activity and effectively inhibited the production of oxidation products in soybean oil during accelerated oxidation, as indicated by reductions in the peroxide value (PV) and acid value (AV). Then, the fatty acids and volatile compounds of soybean oil were determined with gas chromatography–mass spectrometry (GC-MS) and headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS). A total of 108 volatile components, including 16 alcohols, 23 aldehydes, 25 ketones, 4 acids, 15 esters, 18 hydrocarbons, and 7 other compounds, were identified. QG significantly reduced the content and number of aldehydes and ketones, whereas the formation of acids and hydrocarbons was completely prevented. In addition, the fatty acid analysis demonstrated that QG significantly inhibited oxidation of unsaturated fatty acids. Consequently, QG was identified as a potential, new natural antioxidant that is believed to be safe, effective and economical, and it may have potential for use in plant extracts feed additives.
18
Nasr, Alyaa, Xinxin Zhou, Tingting Liu, Jing Yang, and Guo-Ping Zhu. "Acetone-water mixture is a competent solvent to extract phenolics and antioxidants from four organs of Eucalyptus camaldulensis." Turkish Journal of Biochemistry 44, no. 3 (June 12, 2019): 231–39. http://dx.doi.org/10.1515/tjb-2018-0438.
Full textAbstract:
Abstract Objective Eucalyptus camaldulensis is recognized to be one of the major introduced eucalypts, the plant extracts possess a wide range of phytochemicals, which are accountable for its antioxidant and pharmacological activities. The extraction efficiency of such chemical compounds is influenced by the applied extraction conditions. To test the effect of solvent type on the yield of phenolics and antioxidants from E. camaldulensis, seeking for an optimization of the extraction process. Method Dry powders of four plant organs were extracted by some organic solvents besides water. The total phenolic content was detected in the crude extracts of leaf, bud, empty capsule and seed, and was further characterized by GC-MS. Results Acetone-water mixtures yielded high amounts of phenolic compounds with parallel antioxidant activities, correlation coefficients were significant (0.696–0.998). Bud and capsule are first accredited for their antioxidant potentials. The GC-MS analysis revealed the abundance of most detected phenolic constituents in the plant seed. Discussion Acetone-water is a successful and appropriate mixture for the extraction of phenolic compounds from E. camaldulensis. It could give better yields and enhance the biological activities of its corresponding extracts. Conclusion The accurate choice of an extraction solvent has a great influence on the yields of phenolics.
19
bharathi, J. Divya, Dr D. Chandra Prabha, and Dr R. Ragunathan. "Phytochemical screening, invitro antioxidant and anti-inflammatory activity of polyphenolic fraction extracted from Camellia sinensis bud extract." Journal of University of Shanghai for Science and Technology 23, no. 05 (May 13, 2021): 199–210. http://dx.doi.org/10.51201/jusst/21/05134.
Full textAbstract:
Tea is one of the most widely consumed beverages in the world today, second only to water, well ahead of coffee, beer, wine and carbonated soft drinks. The tea plant, Camellia sinensis, is a member of the Theaceae family and black, oolong, white and green tea are produced from its leaf and buds. Tea beverage is an infusion of the dried leaves of Camellia sinensis. The present study aims to screen the phytochemicals screening, in vitro antioxidant and anti-inflammatory activity of polyphenolic compound extracted from Camellia sinensis bud extract. The qualitative phytochemical results revealed the presence of important phytochemicals like alkaloids, flavonoids, phenols, and tannins. The C. sinensis buds could be a rich source of antioxidants. The extract showed significant antioxidant and anti-inflammatory activity. Total phenolic content of the bud’s extract was found to be 123 mg/GAE. The extract possesses a significant antioxidant and anti-inflammatory activity.
20
Shahwan, Ahmed, Naema El Aali, Yusra Layas, and Salem G. El Tumi. "Phytochemical content and antioxidant activities of Rhubarb (<i>Rheum emodi</i>)." Natural Resources for Human Health 2, no. 2 (January 16, 2022): 156–59. http://dx.doi.org/10.53365/nrfhh/144015.
Full textAbstract:
Medicinal plants have varying natural products and several antioxidants. Antioxidants play a principal function to protect against destruction caused by oxidative stress (OS), <i>Rheum emodi</i> is not an exception, in which it is reported to have compounds that possess antioxidant activity, like polyphenolic compounds. In addition to that, other compounds have proven to have antidiabetic, antimicrobial, antifungal, cytotoxic, hepatoprotective, and nephroprotective activities. This study aimed to quantify the water extract of a powdered plant of <i>R. emodi</i> to evaluate its ability to scavage free radicals. Total phenolic, total flavonoid contents, and reducing ability were measured to consider possible sources of future novel antioxidants in plants. The TPC, TFC, and reducing power assay increased with increasing concentrations of <i>R. emodi</i>. At 1000 µg/ml, the absorbance ranged from 1.437 for TPC, 1.602 for TFC, and 1.638 for reducing power assay, which is more than the absorbance of the standards at the same concentration. The extracted content of reducing power, phenolic, and flavonoid compounds were higher than the standard pyrogallol, quercetin, and ascorbic acid.
21
Blasi, Francesca, and Lina Cossignani. "An Overview of Natural Extracts with Antioxidant Activity for the Improvement of the Oxidative Stability and Shelf Life of Edible Oils." Processes 8, no. 8 (August 8, 2020): 956. http://dx.doi.org/10.3390/pr8080956.
Full textAbstract:
Many plant materials, such as fruits and vegetables as well as herbs and spices, represent valuable sources of antioxidants. In recent years, wastes from agriculture and food industrial processes have been shown to be interesting sources for bioactive compound recovery, strongly contributing to the circular economy. Nowadays, because of their possible adverse effects on human health, there is a tendency to replace synthetic antioxidants with natural compounds. This review attempts to critically summarize the current evidence on plant bioactives, extracted from food or waste, added to unsaturated vegetable oils, in order to obtain high added-value products and to ameliorate their oxidative stability and shelf life. The goal of this review is to demonstrate the current status of the research on edible oils added with natural plant bioactives, highlighting new approaches in the field of health-promoting foods.
22
Apurva Choudhary, Rajeshkumar S, Anita Roy, and Lakshmi T. "Free radical scavenging activity of Maranta arundinacea assisted selenium nanoparticles." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (July 25, 2020): 4214–17. http://dx.doi.org/10.26452/ijrps.v11i3.2630.
Full textAbstract:
Selenium nanoparticles have role in toxic waste removal and bioremediation. In a recent study, biosynthesis of many metal and metal oxide nanoparticles by different plant extracts was found to be stable even after wet heat sterilization process. In some studies, biosynthesis of silver, gold, and selenium nanoparticles using microorganisms and plant and parts. In this present study we have synthesized selenium nanoparticles using arrow root and analysed for its free radical scavenging activity. 20 mM of sodium selenite was prepared using 60 mL of distilled water and 40 mL of Arrowroot extract. It was kept in the shaker. Readings were taken for 3 days. Centrifugation was done at 8000 rpm. Pellet was collected and was used for antioxidants activity that is free radical scavenging activity. The antioxidant property of arrow root mediated selenium nanoparticles are comparatively higher than the standard antioxidant property. Calculations were done on the basis of reading that was obtained. This study proves that there is antioxidant activity that is free radical scavenging activity adopted by selenium nanoparticles extracted from arrow root.
23
I M, Dwi Cahya Putra, and Bogoriani N. W. "ANALYSIS OF ANTIOXIDANT ACTIVITY OF LIDAH MERTUA LEAVES (Sansevieria trifasciatalauretii) USING DPPH METHOD." Jurnal Ilmu Pendidikan Indonesia 10, no. 2 (May 24, 2022): 97–109. http://dx.doi.org/10.31957/jipi.v10i2.2195.
Full textAbstract:
Lidah mertua plant has the potential as a natural antioxidant and the antioxidant activity of a plant depends on the number of active compounds extracted. This study aims to determine the yield, total phenol content, total flavonoid content, and IC50 value of lidahmertua leaf extract macerated with ethanol, ethyl acetate, and n-hexane as solvents, and to determine the compounds contained in the lidahmertua leaf extract which have activity antioxidants. The results of the phytochemical test of the lidahmertua leaf extract showed positive results containing polyphenolic compounds, flavonoids, saponins, alkaloids, and steroids. The maceration yield of each solvent was 12.13%, 10.15% and 8.11%, respectively. While the measurement results of the total phenol content of each solvent were 6.96; 3.67; and 1.94 mg GAE/g. The results of the measurement of the flavonoid content of each solvent were 5.82; 3.28; and 1.71 mg QE/g. The IC50 values for each solvent were 162.135 mg/L, 184.561 mg/L and 206.265 mg/L. The extract which has the highest total phenol content, total flavonoid content, and antioxidant activity is in ethanol solvent.
24
SAWICKA, BARBARA HELENA, PARISA ZIARATI, BARBARA KROCHMAL-MARCZAK, and DOMINIKA SKIBA. "Nutraceuticals in food and pharmacy. A Review." Agronomy Science 74, no. 4 (January 7, 2020): 7–31. http://dx.doi.org/10.24326/as.2019.4.1.
Full textAbstract:
The increase in consumer awareness about food and health has led to an increase in the demand for food containing biologically active compounds, especially antioxidants, which can help the human body fight the oxidative stress. Many unconventional or new sources of antioxidants have been discovered, which is a priority not only for food, but also for the pharmaceutical industry. It has been shown that not only raw materials, but also waste from fruit and vegetable processing contains valuable molecules, such as: proteins, essential amino acids, antioxidants, dietary fibers, natural pigments, or aromatic compounds, that can be extracted, purified and modernized in food products or pharmaceuticals with added value. This is the basis of huge potential of not only plant and biotechnology raw materials, but also food waste for the use as a source of antioxidants.
25
Muzykiewicz, Anna, Joanna Zielonka-Brzezicka, and Adam Klimowicz. "Quince (Cydonia oblonga Mill.) as a useful source of antioxidants – antioxidant activity evaluation." Herba Polonica 64, no. 4 (December 1, 2018): 23–33. http://dx.doi.org/10.2478/hepo-2018-0020.
Full textAbstract:
Summary Introduction: Quince (Cydonia oblonga Mill.) is a plant of which both the fruits and the leaves are sources of compounds with antioxidant potential. Such activity could be helpful to prevent the development of so-called oxidative stress. Objective: The aim of the study was to evaluate the antioxidant properties of ethanolic, methanolic and acetonic extracts of mature and immature quince fruits, as well as leaves. Methods: The extracts were prepared using ultrasound-assisted extraction, for 15, 30 and 60 minutes. The antioxidant activity was assessed by DPPH, FRAP, ABTS and Folin-Ciocalteu (F-C) methods. Results: Antioxidant activity of all of the evaluated extracts were observed. The highest potential determined with each method was found for leaf extracts. Moreover, higher activity of unripe fruit extracts compared to ripe fruit was observed. Taking into account the applied extractants, the highest antioxidant capacity was found for methanolic extracts, extracted for 60 and 30 minutes. By contrast, the lowest potential was observed mainly for ethanolic extracts (extraction time 15 minutes). Conclusion: Quince extracts, particularly alcoholic extracts of leaves, seem to be a valuable source of anti-oxidants. Factors as extraction time, the type of solvent and degree of fruit maturity may influence the antioxidant activity of extracts.
26
Ендонова (Endonova), Галина (Galina) Батоевна (Batoevna), Татьяна (Tat'jana) Петровна (Petrovna) Анцупова (Ancupova), and Сысыгма (Sysygma) Дашиевна (Dashievna) Жамсаранова (Zhamsaranova). "CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF MEDICINAL EXTRACTS (SAPONARIA OF-FICINALIS L.)." chemistry of plant raw material, no. 1 (November 14, 2017): 137–43. http://dx.doi.org/10.14258/jcprm.2018012735.
Full textAbstract:
The paper presents new data on chemical composition and antioxidant properties in extracts of the aerial organs of Saponaria officinalis growing on the territory of the Republic of Buryatia. It was revealed that soapberry leaves extracts contain a number of organic acids such as oxalic, formic, fumaric, succinic, malic, citric, and benzoic ones, the dominant being formic, malic, acetic and citric acids. Of vitamins B2, B3. BC extracted from the plant the dominant one was riboflavin. The method of and Fthigh-performance liquid chromatography helped to reveal five compounds identified as aglycone and glycosidic in character. They are orientin, isoeryentin, isovitinexin-7-О-glycoside, orientin-7-О-glycoside, of which isovitoxin is dominant. The greatest total antioxidant activity was demonstrated by soapberry leaves and inflorescences picked up on abandoned sites of the village of Selenginsk. Extractions from Saponaria officinalis leaves showed showed antiradical and Fe2+-chelating activity comparable to that of the extractions from plants with pronounced antioxidant activity. Thus, the data obtained indicated the antioxidant activity of S.officinalis aqueous extracts due to the presence of natural flavonoids. This fact shows the plant as a promising new source of antioxidants.
27
Saleem, Ammar, Markku Ahotupa, and Kalevi Pihlaja. "Total Phenolics Concentration and Antioxidant Potential of Extracts of Medicinal Plants of Pakistan." Zeitschrift für Naturforschung C 56, no. 11-12 (December 1, 2001): 973–78. http://dx.doi.org/10.1515/znc-2001-11-1211.
Full textAbstract:
Abstract Thirty-seven plant organs, traditionally used as drugs, collected in Pakistan, were extracted with 70% acetone and analyzed for their total phenolics concentration and antioxidant potential. Seven extracts showed more than 85% inhibition of lipid peroxidation in vitro as compared with blank. Butylated hydroxytoluene (BHT) (IC50 = 233.6 μg / 1 ± 28.3) was the strongest antioxidant in our test system. The IC50 results indicate that the extracts of Nymphaea lotus L. flowers, Acacia nilotica (Linn.) Delile beans, Terminalia belerica Roxb. fruits, and Terminalia chebula Retz. (fruits, brown) were stronger antioxidants than a-tocopherol, while Terminalia chebula Retz. (fruit coat), Terminalia chebula Retz. (fruits, black) and Ricinus communis L. leaves were weaker antioxidant extracts than a-tocopherol and BHT. Total phenolics concentration, expressed as gallic acid equivalents, showed close correlation with the antioxidant activity. High performance liquid chromatographic analysis with diode array detection at 280 nm, of the seven extracts indicated the presence of hydroxybenzoic acid derivatives, hydroxycinnamic acid derivatives, flavonol aglycones and their glycosides as main phenolics compounds. This information, based on quick screening methods, enables us to proceed towards more detailed chemical and pharmacological understanding of these plant materials.
28
Nowak, Anna, Adam Klimowicz, Wiktoria Duchnik, Łukasz Kucharski, Katarzyna Florkowska, Anna Muzykiewicz, Daria Wira, Joanna Zielonkabrzezicka, Anita Siedłowska, and Kinga Nadarzewska. "Application of green-extraction technique to evaluate of antioxidative capacity of wild population of fireweed (Epilobium angustifolium)." Herba Polonica 65, no. 4 (December 1, 2019): 18–30. http://dx.doi.org/10.2478/hepo-2019-0022.
Full textAbstract:
SummaryIntroduction: Fireweed (Epilobium angustifolium (L.) Holub) is a common weed growing on meadows, roadside and agricultural wasteland, creating vast, rapidly spreading fields.Objective: The aim of the study was to evaluate the antioxidant activity of extracts from fresh fireweed, harvested at three ripening stages.Methods: Analysis of antioxidative activity was carried out by DPPH, ABTS and FRAP methods. Total polyphenol and total flavonoid content were also determined. Plant material was extracted using ultra-sound-assisted green extraction technique with methanol, ethanol and isopropanol at different concentrations and water.Results: The highest antioxidant activity evaluated by DPPH, ABTS and FRAP was found for the extracts prepared in 70% ethanol. The highest content of total polyphenols were observed in extracts in 70% ethanol, whereas the highest content of flavonoids extracts in undiluted methanol.Conclusion:Epilobium angustifolium harvested at fruit ripening stage seems to be a valuable source of antioxidants.
29
Taghvaei, Mostafa, Seid Mahdi Jafari, Alireza Sadeghi Mahoonak, Alireza Mehregan Nikoo, Neda Rahmanian, Javad Hajitabar, and Nasim Meshginfar. "The effect of natural antioxidants extracted from plant and animal resources on the oxidative stability of soybean oil." LWT - Food Science and Technology 56, no. 1 (April 2014): 124–30. http://dx.doi.org/10.1016/j.lwt.2013.11.009.
Full text
30
Kannan, K. Suresh, D. Kandavel, and P. Rajalakshmi. "In vitro Assessment of Antioxidant and Anticancer Potential of Flavonoid Glycosides of Glinus oppositifolius (L.) Aug. DC." Asian Journal of Chemistry 34, no. 4 (2022): 1003–8. http://dx.doi.org/10.14233/ajchem.2022.23664.
Full textAbstract:
Development of unbalanced state of free radicals and their elimination in the body is the reason for increase in different types of chronic diseases including cancer. A better natural supplement to play role of antioxidants to combat the free radicals and to reduce the risk of cancer is in high demand. This current research work focused on identifying such a potent phytocompounds from Glinus oppositifolius (L.) Aug. DC. The active compounds of the aerial parts of the plant have been extracted using methanol and the phytochemical analysis was carried out using qualitative tests, FTIR and GC-MS. The plant extract found to contain range of phyto compounds with good amounts of flavonoid and xylofuranoside. Flavonoid glycoside from the plant extract has been partially purified through chromatography and tested for efficiency against free radicals through in vitro antioxidant assays. The partially purified compound showed good antioxidant activity equivalent to 62.12 to 63.35 μg of rutin standard. Further cytotoxic nature of the flavonoid glycoside was also assessed against breast cancer MDA-MB-231 cell line. The tested compound has shown cancerous cell controlling ability (76.68% cell death in 24 h). The immense antioxidant and anticancer potential of flavonoid glycosides of G. oppositifolius could be utilized as a good source of natural, antioxidant supplement under nutraceuticals to prevent oxidative-stress-related disorders and also as therapeutic compound to cure cancer under pharmaceuticals.
31
NOUREEN, Shazia, Sobia NOREEN, Shazia Akram GHUMMAN, Fozia BATOOL, Mahira ARSHAD, Fozia NOREEN, Umair ISHTIAQ, and Syed Nasir Abbas BUKHARI. "Seeds of Giant Dodder (Cuscuta reflexa) as a Function of Extract Procedure and Solvent Nature." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 46, no. 2 (March 17, 2018): 653–62. http://dx.doi.org/10.15835/nbha46211088.
Full textAbstract:
Seeds of a renowned medicinal plant, giant dodder (Cuscuta reflexa), were assessed to appraise the effect of solvent and extraction technique variation on antioxidants potential. Dodder seed, also called cuscuta seed, has been considered superb tonic in traditional herbal medication for eyes, liver, spleen and kidney. Results show that selected solvent and procedure plays a key role in the composition and activity of extractable material. Three extraction procedures Orbital shaker, Decoction and Ultrasonic assisted extraction and five different solvents n-hexane, ethyl acetate, 100% methanol, 80% methanol and 60% methanol were used to get optimized conditions. Total phenolic and flavonoids content were found maximum in the extracts of aqueous organic system containing 80% methanol in Ultrasonic assisted extraction method but in case of tannins ethyl acetate and Orbital shaker extraction was found more suitable partner. Antioxidant estimation assays showed a little bit variation as DPPH and ABTS exhibited maximum inhibition in 80% methanol and Ultrasonic assisted extraction but 100% methanol was found better for FRAP assay. Decoction results were mostly in between the both Orbital shaker and Ultrasonic assisted extraction. Overall results indicate that coexistence of polar solvents and Ultrasonic assisted extraction gives a better choice for extractability of potent antioxidants from seeds. HPLC analysis confirmed presence of valuable phenolic acids. Pearson’s correlation coefficient reveals a significant relationship between extracted components and antioxidant capacity P< 0.05 or 0.01.
32
Ajeena, Saba J. "Effect of Bay leaves (Laurus nobilis) Plant Extracts as Anti-Oxidant on Crud Sun flower Oil." Iraqi Journal of Veterinary Medicine 36, no. 2 (December 31, 2012): 111–18. http://dx.doi.org/10.30539/iraqijvm.v36i2.455.
Full textAbstract:
This investigation was carried out to study the Bay leaves (Laurus nobilis) and extracted three types of Aqueous, Ethanolic alcohol, Essential oil extracts of leaf plant powder study. The performance of extracts as anti-oxidant agent were evaluated by estimate the value of Thiobarbituric acid (TBA) of sunflower oil to these extracts in concentrate% 0.04 and %0.02 compared with commercial antioxidant BHA (Butylated hydroxyl anisole) and BHT (Butylated hydroxyl toluene) in concentrate % 0.02 , in addition to the control sample (of crude sunflower oil without any additives) during 16 days storage at 60 C. The results showed an increase in the TBA values in sunflower oil, crude and stored in during storage at 16 days in 60 C. Some extracts laurels showed high anti-oxidant activity and more than commercial antioxidants (BHT) and (BHA). The results of the TBA transactions for crude extracts of plant concentration% 0.04 most efficient overall transactions in concentration % 0.02, aqueous extract of bay leaves in the concentration of % 0.04 showed high activity asan antioxidant with 2.88 mg Malonaldehyde / kg oil TBA value in the 16th and the last day of storage time in 60 C, while the TBA values of the rest of the coefficients of crude sunflower oil translate with plant extract :essential oil ethanolic alcohol and water extract in the concentration of 0.02% were 4.04, 3.39, 3.12 mg Malonaldehyde / kg oil, and in the concentration of 0.04% for essential oil ethanolic alcohol treatment were 4.01 and 2.29 mg Malonaldehyde / kg oil , respectively in the last day of storage, either control and commercial antioxidants BHA and BHT reached TBA values of crude oil 6.88, 3.16 and 3.50 mg Malonaldehyde/kg oil, respectively, in the same day of storage. Was estimated as the content of plant nutrients and mineral major and minor, were detected. Chemical quality of the active chemical compounds of plant leaves, which included tannin, Alclaicosadat, Alsabonyat, resins, phenols, flavonoids and alkaloids were evaluate to.
33
Nzaramba, M. N., Douglas C. Scheuring, and J. Creighton Miller. "VARIABILITY FOR ANTIOXIDANT ACTIVITY IN THE U.S. COWPEA [Vigna unguiculata (L) WALP.] CORE COLLECTION." HortScience 40, no. 3 (June 2005): 881d—881. http://dx.doi.org/10.21273/hortsci.40.3.881d.
Full textAbstract:
Antioxidants are important to human health, as they are responsible for reduced risk of diseases such as cancer, hence motivating researchers to examine crop plants for available antioxidant compounds. There is also increasing interest in the use of antioxidants from plants instead of synthetic products. In order to evaluate variability of antioxidant activity (AOA) in cowpea, 697 cowpea accessions from the U.S. Cowpea Core Collection obtained from the Regional Plant Introduction Station, Griffin, Ga., were analyzed for AOA expressed as μg trolox equivalents/gdw. Two grams of dry seed from each accession were ground, extracted in methanol and analyzed for AOA using the free radical, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), method. A large variation in AOA within the core collection, ranging from 1859 μg·g–1 dw (PI 180355, pigmented seed coat) to 42.6 μg·g–1 dw (PI 583100, cream seed coat), was observed. A least significant difference of 131.5 (p =0.05) was obtained. Higher AOA was manifested by accessions with pigmented seed coats. Accessions that were speckled, striped or had a pigmented eye were moderate in AOA, while the cream types were generally low. Variability in AOA observed among cowpea accessions suggests that breeding for high AOA can be successfully conducted. Accessions with high AOA could also be used to extract antioxidants for industrial purposes. Some accessions were a mixture of various colors and patterns, making it difficult to classify them into a particular category. Therefore, there is need to ensure purity of these accessions by ascertaining whether the mixtures are physical, i.e., combination of different varieties, or are composed of segregating material.
34
Sumartini and P. W. Ratrinia. "Nutrition of wet noodles with mangrove fruit flour during the shelf life by adding catechins as a source of antioxidants." IOP Conference Series: Earth and Environmental Science 967, no. 1 (January 1, 2022): 012015. http://dx.doi.org/10.1088/1755-1315/967/1/012015.
Full textAbstract:
Abstract Wet noodles are foods that are very popular with the public, but basically, noodles do not contain many important nutrients. Catechins are a class of antioxidant compounds that can be extracted from various plant sources. This goal from this research is making of noodles from mangrove fruit flour with the addition of catechins is carried out to increase the nutritional and nutritional value and improve the quality of wet noodles during the shelf life. The research method used an experimental design with a factorial Completely Randomized Design (CRD) with three factors (K = Control, S = T. Mangrove fruit S. caseolaris). A (T. Mangrove fruit A.marina) and 3 test levels, namely storage time (0.3 and 5 days). The results were processed using SPSS version 22 software with one-way ANOVA. The results showed the value of water content in the range of 46.33-56.82%, protein content 3.88-6.83%, fat content 1.76-2.82%, ash content 0.04-0.18%, levels of carbohydrates 39.08-48.79%, fiber content 3.09-8.81%, antioxidant activity 5.11-47.74%. The results showed that seaweed fruit flour wet noodles with catechins as a source of antioxidants were able to improve the quality and nutrition of wet noodles during the shelf life. The results showed that the manufacture of wet noodles from mangrove fruit flour with the addition of 7% catechins as a source of antioxidants was able to significantly improve the nutritional quality and nutrition of wet noodles during the shelf life compared to control (K) wet noodles with wheat flour as raw material without 7% catechins as a source of antioxidants
35
SHARMA, Arun Dev, Sooraj AGNISH, and Inderjeet KAUR. "Chemical composition analysis and biological activities of essential oil from Eucalyptus polybractea (L.), growing in plains of Punjab, Northern India, by two different hydro-distillation methods." Notulae Scientia Biologicae 14, no. 1 (March 18, 2022): 11191. http://dx.doi.org/10.15835/nsb14111191.
Full textAbstract:
Eucalyptus essential oil poses various biological activities due to the presence of various bioactive compounds. The objective of this study was to evaluate the effect of direct heat-based hydro distillation (DHBH) and in-direct heat-based hydro-distillation (IDHBH) methods on chemical profile, extraction, and biological activities of essential oils from Eucalyptus polybractea. Eucalyptus Essential Oil (EEO) was extracted from green leaves of eucalyptus plant using DHBH and IDHBH methods. Aroma profile was evaluated by GC-FID technique. Content of phenolics, flavonoids and tannins accompanied by antioxidant activities like iron-chelation and DPPH were studied. Other biological activities like anti-inflammatory and anti-microbial were also analysed. GC-FID chemical profiling revealed a greater number of bioactive compounds in IDHBH extract than DHBH. Relationship between antioxidant activity and chemical composition of extracted oils was investigated. Significant amount of β- pinene, 1,8 cineole, eugenol, Pentadecanoic acid are responsible for antioxidative capacity of both extracts. The concentration of all compounds, especially Pentadecanoic acid and other unique bioactives were higher in IDHBH extract. The level of antioxidants like phenolics, flavonoids and tannins by was high also high in IDHBH extracted oil. This corresponds to higher antioxidant activities like DPPH and iron-chelating activity of IDHBH oil compared with DHBH extract. Similarly, anti-inflammatory, and anti-microbial potential was more in oil extracted with IDHBH than DHBH. The present paper reported that different extraction methods lead to different biochemical composition of essential oils and the choice of a suitable method is extremely important to obtain more preferred compounds with more potent biological activities and this method can be the choice for essential oil-based companies.
36
Demirhan, İlter, Büşra Çitil, Mehmet Özyurt, Meltem Güngör, Erkan Öner, and Ergül Belge Kurutaş. "Determination of in Vitro Antioxidant Enzyme Capacity and Oxidative Stress Levels in Mazı Meşesi (Quercus infectoria)." Turkish Journal of Agriculture - Food Science and Technology 9, no. 4 (April 26, 2021): 814–17. http://dx.doi.org/10.24925/turjaf.v9i4.814-817.4254.
Full textAbstract:
South East Anatolia Region has a large genetic plant diversity due to its physical and different climatic charesteristics. These plants are potential sources of antioxidants that prevent oxidative stress caused by oxygen and photons. In recent years, it has become important to study the antioxidant capacity of many molecules found naturally in foods and biological systems. The reason for this is that it is believed that when the consumption of food rich in antioxidants is increased, the risk of developing different degenerative diseases will be reduced. In this study, it was aimed to measure the antoxidant capacity of Quercus infectoria, G.olivier gal seeds grown in Southeastern Anatolia. Q. infectoria gal seeds from Sanlıurfa province were used in our study. Q. infectoria gal seeds were extracted with water, ethanol and methanol and then antioxidant enzyme activities (catalase and superoxide dismutase) and malondialdehyde levels, which are indicators of oxidative stress were determined by spectrophotometric methods. It was found that the antioxidant capacity (catalase and superoxide dismutase activities) of extracts obtained from ethanol and methanol were higher and their malondialdehyde levels were statistically lower than those obtained from water. However, it was determined that there was no statistically significant difference between the antioxidant capacity and malondialdehyde levels of the extracts obtained from methanol compared to the extracts obtained from ethanol. It has been concluded that Q. infectoria gal seed has a effective antioxidant effect. In addition, it was observed that extracts obtained from ethanol and methanol have higher antioxidant capacity than extracts obtained from water.
37
Essa, Hanaa L., Hania A. Guirguis, Mayyada M. H. El-Sayed, Dalia Rifaat, and Mohamed S. Abdelfattah. "Ultrasonically-Extracted Marine Polysaccharides as Potential Green Antioxidant Alternatives." Proceedings 67, no. 1 (November 9, 2020): 23. http://dx.doi.org/10.3390/asec2020-07606.
Full textAbstract:
Marine-extracted sulfated polysaccharides (SPs) have been the subject of myriad research since they are considered an eco-friendly source of biologically active compounds. Meanwhile, food and pharmaceutical industries are urgently producing natural sugar substitutes and antioxidants as alternatives to synthetic ones which are associated with cytotoxicity and safety issues. This study assesses the potential of using marine SPs obtained via the ultrasonic-assisted extraction of different marine species, to utilize them as antioxidant sugar substitutes. The carbohydrate, total phenolic contents and antioxidant activities were measured for SP extracts of the algal species of Ulva lactuca, Jania rubens and the marine plant mangrove Avicennia marina. These SPs were structurally elucidated by Fourier Transform Infrared (FTIR) spectroscopic and high-performance liquid chromatography (HPLC) analyses. The results revealed that SPs’ highest yield percent was obtained from Ulva lactuca, 5.50 ± 0.25%. The SPs of Avicennia marina had the highest carbohydrate content, 44 ± 1% and antioxidant activity, 78.85 ± 0.06 at the 100 μg/mL concentration and 89.50 ± 0.21 at the 250 μg/mL concentration. Meanwhile, the highest phenolic content was exhibited by algal SPs obtained from Jania rubens, 132.60 ± 2.50 mgGa/g. Results also showed that all extracts have potent antioxidant activity, while the highest antioxidant activity belonged to the SPs of Avicennia marina owing possibly to their balanced glucose and galactose contents as measured by HPLC. This work emphasizes the need to consider sulfated polysaccharides from marine sources for their antioxidant activity and to correlate it with their monosaccharide content to determine the effect of reducing sugar concentration on the antioxidant activity.
38
Hassan, Amir, Zakaria Akmal, and Nawaz Khan. "The Phytochemical Screening and Antioxidants Potential of Schoenoplectus triqueter L. Palla." Journal of Chemistry 2020 (September 30, 2020): 1–8. http://dx.doi.org/10.1155/2020/3865139.
Full textAbstract:
Over the centuries, humans use different types of therapeutic plants to treat several diseases. Cyperaceae family has a significant number of monocotyledon plants, and Schoenoplectus is one of the genera that belong to this family; about forty-nine compounds are isolated. Our current study was evaluated on Schoenoplectus triqueter L. Palla to show the potential of its antioxidants and confirm the phytochemical constituents in this plant species. Fully powdered plant taken for successive extraction process in hot continuous process for Soxhlet was 20 g plant in porous bag manually prepared; the constant temperature provided was 40–50°C. In the maceration extraction method, 30 g plant was taken in a closed jar and the solvent placed for extraction was 300 mL of ethanol; the extract gets filtered and fractioned to different solvents such as water, dichloromethane, ethyl acetate, and n-hexane fraction. Important types of phytochemicals found in this species are alkaloids, proteins, amino acids, flavonoids, phenols, terpenoids, tannins, saponins, and carbohydrates. All the entire extracted fractions which are water, dichloromethane, ethyl acetate, and n-hexane possess noticeable activity at various concentrations of 31.25, 62.5, 125, 250, and 500 µg/mL by the dilution method. The ethyl acetate extract holds greater median inhibitory concentration (IC50 = 3.52 ± 0.01), and water showed IC50 = 3.61 ± 0.01 percent potential as compared to the standard ascorbic acid which possesses IC50 = 2.27 ± 0.01. Their potential may be enhanced or lowered with the purification of extracts which might be useful in biological activities.
39
Tariq Amir Najar, Suchitra Banerjee, and Rajendra Chauhan. "In-Vitro Antioxidant Activity of Total Phenolic and Flavonoid Content of Solanum nigrum Leaf." International Journal for Research in Applied Sciences and Biotechnology 9, no. 2 (March 25, 2022): 96–106. http://dx.doi.org/10.31033/ijrasb.9.2.12.
Full textAbstract:
Solanum nigrum belonging to the family Solanaceae, has been considered as a remedy for treating various ailments like epilepsy, gastric ulcers etc. and is believed to have hepatoprotective activities. In this study, the total phenolic and flavonoid content of the extracts of Ethyl acetate and Methanol from the Solanum nigrum leaves and their antioxidant activity was carried out by using spectrophotometric methods. Methanol extract with high absorbance exhibited highest antioxidant activity containing highest quantity of phenolics and flavonoids followed by Ethyl Acetate. The presence of phenolic and flavonoid compounds indicated that these compounds can contribute to the antioxidant activity. These active constituents alone or in combination with others may be responsible for the observed antioxidant activity. The findings indicated promising antioxidant activity of crude extracts of leaves needs further exploration for their effective use in both modern and traditional system of medicines. The Solanum nigrum leaf can be regarded as promising candidate for natural plant sources of antioxidants with higher values. A comparative study of the total phenolic and flavonoid contents extracted by the solvents, and their in-vitro antioxidant activity that could be an effective remedy in hepatoprotection was carried out in the study.
40
Jayaprakasha, G. K., Clark Wilson, and Bhimanagouda S. Patil. "Phenolics and Carotenoids Contribute to Antioxidant Activity in Navel Orange." HortScience 41, no. 4 (July 2006): 1000A—1000. http://dx.doi.org/10.21273/hortsci.41.4.1000a.
Full textAbstract:
Consumption of fruits and vegetables has been associated with reduced risk of disease, such as neurodegenerative disorders and certain forms of cancer, and aging. Antioxidants prevent the damage to macromolecules and cells by interfering with the free radicals. Several natural compounds that posses antioxidant activity have been reported from plant sources and are commercially promoted as nutraceuticals. Citrus fruits contain certain bioactive compounds such as phenolics, flavonoids, limonoids, carotenoids, and ascorbic acid. In this context, navel oranges were freeze-dried and extracted with five different solvents, such as hexane, ethyl acetate, acetone, methanol, and 8 methanol: 2 water. The extracts were dried under vacuum and screened for their radical scavenging activity using the 1,1-diphenyl-2-picrylhydrazyl method at 250, 500, and 1000 ppm. The methanol: water and methanol extracts of navel orange were found to be maximum (92.9%) and minimum (63.89%) radical scavenging activity at 1000 ppm. Furthermore, the antioxidant capacity of all extracts was assayed through the phosphomolybdenum method and expressed as equivalent to ascorbic acid (μmol/g of the extract). The order of antioxidant capacity for navel orange extracts was found to be ethyl acetate > acetone > methanol: water > methanol > hexane. It seems that the antioxidant capacity of the extracts is in accordance with the amount of phenolics/lycopene present in each fraction and may provide a good source of antioxidants. This project is based upon work supported by the USDA–CSREES under Agreement USDA IFAFS #2001-52102-02294 and USDA #2005-34402-14401 “Designing Foods for Health” through the Vegetable and Fruit Improvement Center.
41
Owoade, Abiodun O. "Phytochemical Characterization and Antioxidant Bioactivity of Andrographis paniculata (Nees)." Pan African Journal of Life Sciences 5, no. 2 (August 31, 2021): 246–56. http://dx.doi.org/10.36108/pajols/1202.50.0220.
Full textAbstract:
Background: Medicinal plants are natural sources of antioxidants effective in the treatment of radical mediated diseases. This study evaluated the in-vitro antioxidant and phytochemical constituents of the methanolic leaves extract of Andrographis paniculata . Methods: Fresh A. paniculata leaves were harvested from a local far m, air -dried and extracted with methanol. Chemical composition, antioxidant activities, and α-amylase enzyme inhibitory potentials of the extract were determined Results: The extract of A. paniculata concentration-dependently scavenges 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2’azinobis (3-ethylbenzothiazoline-6-sulphonic acids) (ABTS) radicals. It scavenges nitric oxide radicals with IC50 of 145.99 μg/ml compared to 167.17 μg/ml of standard ascorbic acid and has 41% activity of standard ascorbic acid ferric reducing power. The extract also inhibited the induction of lipid peroxidation and α-Amylase activity in a concentration-dependent manner. The phytochemical assays employed revealed the presence of various phytochemicals in the extract. Further analysis with gas-chromatography revealed the possible presence of Andrographolide, Deoxyandrographolide, Apigenin, Kaempferol, Quercetin, Methyl vanillate, Methyl Caffeate, Beta-sitosterol, Vanillic acid in the extract. The total phenolics content was found to be 29.11mg GAE/g, and proximate analysis revealed the moisture content, crude protein, crude fat, crude fiber, total ash, and Nitrogen free extract to be 21.89%, 5.66%, 8.74%, 0.95%, 6.87%, and 55.89% respectively. Conclusion: The plant A. paniculata demonstrated good antioxidant potentials and contain various phytochemicals. Therefore, it could be inferred that the effectiveness of A. paniculata as a medicinal plant could be due to the presence of various phenolics and antioxidant compounds in the plant.
42
Abubakker, Nishadh. "EVALUATION OF ENZYMIC AND NON-ENZYMIC ANTIOXIDANT LEVELS IN VERNONIA CINEREA EXTRACTS." Asian Journal of Pharmaceutical and Clinical Research 11, no. 7 (July 7, 2018): 167. http://dx.doi.org/10.22159/ajpcr.2018.v11i7.22109.
Full textAbstract:
Objective: The objective of this study was to evaluate the enzymic and non-enzymic antioxidant levels in various solvent extracts of Vernonia cinerea leaves.Methods: The fine powder of leaf (180 g) was extracted successively with methanol, ethanol, petroleum ether (40–60°C), benzene, acetone, ethyl acetate, chloroform, and aqueous in a Soxhlet extractor for 18 h. The extracts were concentrated under reduced pressure at low temperature (40– 50°C), and the extracts were analyzed for the antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), peroxidise, glutathione S-transferase (GST), glutathione peroxidase (GPx), polyphenol oxidase, glutathione (GSH) reductase, and glucose-6-phosphate dehydrogenase and non-enzymic antioxidants such as Vitamin A, C, E, reduced GSH, and total phenol.Results: Significant activities of enzymic antioxidants such as CAT (23.68 μ mole of H2O2 decomposed/min/mg protein, SOD (19.75 inhibition of 50% nitrite form/min/mg protein), and GST (73.28 μ mole of 1-chloro-2,4-dinitrobenzene conjugate formed/min) were observed higher in the methanolic extracts. Whereas, ethanolic extract exhibits maximum activity of GPx (1.054 μ mole of GSH utilized/min) and Px (102.1 μ mole of pyrogallol oxidized/ min/mg protein). Total GSH (172.3 μM/g), Vitamin E (23.76 μM/g), and total phenols were significantly predominant in the ethanolic extracts followed by methanol and ethyl acetate extracts.Conclusion: V. cinerea seems to be a promising plant in respect of its antioxidant potential, there is a lot more to be done to understand the mechanisms behind these effects as well as to employ them as possible therapeutic agents.
43
Mendili, Moahamed, Mohamed Ali Smach, Nedaa Husein, and Ayda Khadhri. "Comparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo." Avicenna Journal of Medical Biochemistry 8, no. 2 (December 30, 2020): 64–73. http://dx.doi.org/10.34172/ajmb.2020.10.
Full textAbstract:
Background: Maclura pomifera a member of Moraceae family, is a tropical plant with ethnobotanical uses. Objectives: The present study aimed to evaluate bioactive compounds, antioxidants, and acetylcholinesterase (AChE) inhibitory activities of different extracts of Tunisia M. pomifera (Rafin.) Schneid fruit in vitro and in vivo. Methods: Organic extracts in different polarities (chloroform, ethyl acetate, and acetone) were extracted from different parts of the fruit of M. pomifera (exocarp, mesocarp, and pips). Phenolic content, antioxidant activity, and anti-AChE activity were determined. The anti-amnesic effects of ethyl acetate extract of the exocarp of M. pomifera were measured in galactose-induced memory deficit mice by the Y maze. The levels of biomarkers and AChE activity were determined in brain tissues. Results: The obtained results showed that the ethyl acetate extract of exocarp contains the highest content of flavonoids and polyphenols 22.3mg quercetin equivalents per g of dry weight and 718.6 mg gallic acid equivalents per g of dry weight. The evaluation of antioxidant activities highlighted that the ethyl acetate extract of exocarp was the most active element. The study of the AChE inhibitory power demonstrated that the ethyl acetate extract of the exocarp had the greatest inhibitory activity. The ethyl acetate extract from the exocarp ameliorated cognitive performance and reversed the oxidative damage as compared to galactose group. Conclusion: M. pomifera fruit is a good source of natural antioxidants, which might help prevent oxidative stress-related damage and memory impairment in such mental disorders as Alzheimer’s disease (AD).
44
Święciło, Agata, and Kamila Rybczyńska-Tkaczyk. "Resazurin Method for Evaluation of Bioactive Compounds from Cranberry Extracts Using the Metabolic Activity of a ΔSOD1 Mutant of Saccharomyces cerevisiae Yeast Under Severe Osmotic Stress." Journal of AOAC INTERNATIONAL 103, no. 2 (March 2020): 422–27. http://dx.doi.org/10.5740/jaoacint.19-0264.
Full textAbstract:
Abstract Background: In addition to nutrients, plant raw materials for food production should also contain substances with beneficial biological properties, which unquestionably include antioxidant compounds. Among the numerous methods of determining the antioxidant properties of samples of plant material, biological methods that provide information about not only the in vivo antioxidant potential of samples but also their metabolism and bioavailability are increasingly valued. Objective: The aim of the study was to assess the antioxidant properties of extracts from large cranberry (Vaccinium macrocarpon) obtained from different producers. Methods: Biologically active compounds were extracted from cranberry fruits using water alone and ethyl alcohol–water in proportions of 1+1 and 4+1 (v/v) as solvents. The following were determined in the extracts: content of phenolic compounds and anthocyanins, total antioxidant capacity based on reduction of the ABTS+• [2,20-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] radical cation, and antioxidant properties as reflected by the growth of a Saccharomyces cerevisiae Δsod1 mutant in a liquid hypertonic environment. The growth parameters of this Δsod1 mutant, monitored by a method exploiting a color reaction with resazurin, reflected the antioxidant properties of the extracts. Results: The ethanol–water cranberry extracts showed higher content of polyphenols, anthocyanins, and total antioxidants expressed as Trolox equivalent, determined on the basis of ABTS+• reduction. Conclusions: The antioxidant properties determined by the bioassay did not respond strongly to the data obtained in the in vitro chemical and biochemical assays, because they were more closely associated with the batch of fruit than with the type of solvent used to extract phytochemicals.
45
Girennavar, Basavaraj, Bhimanagouda Patil, and Guddadarangavvanahally Jayaprakasha. "Antioxidant Activity of `Rio Red', `Marsh White', and Commercial Juice In Vitro Model Systems." HortScience 40, no. 4 (July 2005): 1135B—1135. http://dx.doi.org/10.21273/hortsci.40.4.1135b.
Full textAbstract:
Antioxidant activity is widely used as a parameter to characterize different plant materials for potential health benefits. This activity is related with compounds capable of protecting a biological system against the harmful effect of reactions that can cause excessive oxidation, involving reactive oxygen and nitrogen species (RONS). There has been growing interest in the beneficial health effects of consuming fruits and vegetables. Mainly, the presence of lycopene, ascorbic acid, and phenolic antioxidants is believed to have the protective mechanism. The free radical-scavenging activities of grapefruit extract of `Rio Red', `Marsh White', and commercial juice were extracted with different solvents, such as hexane, ethyl acetate, and chloroform. The dried extracts were screened for their radical scavenging activity using the α,α -diphenyl-β-picrylhydrazyl (DPPH) method. The ethyl acetate extracts of commercial juice and `Marsh White' were found to possess more radical scavenging activity compared with the other two extracts. However, chloroform extracts of `Rio Red' grapefruit were the most active, which may be ascribed to the presence of more lycopene. Furthermore, the antioxidant capacity of `Rio Red' and `Marsh White' extracts was assayed through the phosphomolybdenum method and expressed as equivalent to ascorbic acid (μmol·g-1 of the extract). The order of antioxidant capacity for `Rio Red' extracts was found to be hexane > chloroform > ethyl acetate, while the order for `Marsh White' was chloroform > hexane > ethyl acetate. The results indicate that the extent of antioxidant activity of the extract is in accordance with the amount of lycopene/phenolics present in that extract; commercial juice and `Rio Red' may provide a good source of antioxidants.
46
Reddy Palvai, Vanitha, Sowmya Mahalingu, and Asna Urooj. "Abrus precatorius Leaves: Antioxidant Activity in Food and Biological Systems, pH, and Temperature Stability." International Journal of Medicinal Chemistry 2014 (January 30, 2014): 1–7. http://dx.doi.org/10.1155/2014/748549.
Full textAbstract:
Natural antioxidants present in foods and other biological materials have attracted considerable interest because of their presumed safety and potential nutritional and therapeutic effects. Antioxidant constituents of plant materials act as radical scavengers and convert the radicals to less reactive species. Abrus precatorius (AP) was analyzed for its proximate and phytochemical composition. The leaves were extracted with methanol (ME) and analyzed for antioxidant activity by radical scavenging method, reducing power, ferric reducing capacity, and in vitro inhibition of Fenton’s reagent-induced oxidation in oil emulsion and microsomes. In addition, the effect of temperature (100∘C, 15, and 30 min) and pH (4.5, 7, and 9) C on the antioxidant activity of ME was investigated. The leaves were rich in total polyphenols, flavonoids, β-carotene, glutathione, α-tocopherol, and ascorbic acid. The ME exhibited varying degree of antioxidant activity in a dose-dependent manner. The AP exhibited more inhibition of oxidation in microsomes (73%) than compared to oil emulsion (21%). Heat treatment resulted in an increase of radical scavenging activity of extract (28% to 43%). At pH 4.5 the extract exhibited more antioxidant activity and stability compared to pH 7 and 9. Data indicates that potential exists for the utilization of Abrus precatorius as a natural antioxidant.
47
Rudiana, Tarso, Dhyneu Dwi Jayanti, and Siti Solehah. "Profile Secondary Metabolite Compounds and Antioxidant Activities of Stem Bark Jambu Mete (Anacardium occidantale L.) Extract." Jurnal Akademika Kimia 10, no. 3 (August 30, 2021): 133–38. http://dx.doi.org/10.22487/j24775185.2021.v10.i3.pp133-138.
Full textAbstract:
Oxidative stress is a condition in which the amount of free radicals and antioxidants in the body is not balanced, causing various diseases. One of them is a degenerative disease. Oxidative stress can be reduced by antioxidant compounds. Anacardium occidentale L. is a type of plant from the Anacardiaceae family. This plant is found in many trophic countries included Indonesia, and is believed to ward off degenerative diseases caused by oxidative stress. The purpose of this study was to analyze the activity of A. occidentale stem bark extract against DPPH radical inhibition (1,1-Diphenyl-2-Picrilhidrazil) and the profiling of secondary metabolites in their active fraction. The bark of A. occidentale was extracted by a gradient maceration method. The solvents used were n-hexane (nonpolar), ethyl acetate (semi-polar), and methanol (polar). The macerate was concentrated using a rotary vacuum evaporator, followed by DPPH radical inhibition analysis using a spectrophotometer. The most active extract was analyzed for chemical content by LC-MS/MS. The results showed that the extract of A. occidentale stem bark had antioxidant activity. Methanol extract obtained very strong and best antioxidant activity with an IC50 value of 47.13 ppm compared to ethyl acetate extract and n-hexane extract, which had IC50 values of 163.92 ppm and 501.72 ppm, respectively. Secondary metabolites that are thought to have antioxidant activity in the methanol extract of A. occidentale stem bark were epigallocatechin (EC) and epigallocatechin gallate (EGCG) compounds.
48
Kanthang, Supha, and Kanokporn Sompornpailin. "Antioxidant Activity in the Petal Extract of PAP1 Transgenic Tobacco." Applied Mechanics and Materials 804 (October 2015): 195–98. http://dx.doi.org/10.4028/www.scientific.net/amm.804.195.
Full textAbstract:
Flavonoids are compounds which act as antioxidants in both plant and human. These substances are found in reproductive tissues. Tobaccos expressing ORF of PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1) involved in flavonoid biosynthesis were used for investigating the flavonoid profiles and antioxidant activity of petals and compared to wild type (WT) tobacco. Approximately two months, the tobacco grown in a culturing room had produced flowers for harvesting. The petal tissues were extracted with the solvent of acidic methanol and water. These extracts were measured the specific wavelength of flavonoid derivatives by using a spectrophotometer. The petal extract of H7 line showed the highest content of all detected flavonoid subgroups. However the petal extract of transgenic line H5 and H7 had significantly higher levels of anthocyanin (pelargonidin) than those of the other transgenic and WT tobaccos. An antioxidant activity of the petal extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) assay. An effective concentration of the extract which scavenged DPPH radical by 50% (EC50) was presented. EC50 of the H5 and H7 transgenic extracts was approximately two folds less than that of WT extract Therefore anthocyanin may highly affect on antioxidant activities.
49
Attar, Usmangani A., and Savaliram G. Ghane. "Proximate composition, antioxidant activities and phenolic composition of Cucumis sativus forma hardwickii (Royle) W. J. de Wilde & Duyfjes." International Journal of Phytomedicine 9, no. 1 (March 30, 2017): 101. http://dx.doi.org/10.5138/09750185.1922.
Full textAbstract:
In the present investigation, nutritional, anti-nutritional and antioxidant potential of Cucumis sativus forma hardwickii was evaluated for the first time. The results revealed that leaf and fruit of Cucumis sativus f. hardwickii were rich source of ash, carbohydrate, fat, fibre and proteins. In addition, appreciable amounts of Vitamin C and E were also observed. Considerable antinutritional factors in leaf and fruit were noted which can be reduced by different processing methods. In sequential solvent extraction, methanol found to be the best in the extraction of antioxidants, which was supported with the highest antioxidant activities. Among all ¬¬the extracts, it was noted that leaves extracted with methanol had higher amount of phenols and flavonoids than fruit. In addition, the potent radical scavengers mainly phenolics were detected using RP-HPLC where tannic acid, gallic acid and hydroxybenzoic acid were also quantified in leaf and fruit. Superior antioxidant potential may be due to the presence of other bioactive compounds present in fruit. Additional research on bioactive properties of this plant is needed to explore this neglected wild cucumber for nutraceutical purpose and as a functional food.
50
Muzhingi, Tawanda, Kyung-Jin Yeum, Andrew H. Siwela, Odilia Bermudez, and Guangwen Tang. "Identification of Enzymatic Cleavage Products of β-Carotene-Rich Extracts of Kale and Biofortified Maize." International Journal for Vitamin and Nutrition Research 87, no. 5-6 (September 1, 2017): 279–86. http://dx.doi.org/10.1024/0300-9831/a000437.
Full textAbstract:
Abstract. Provitamin A carotenoids in plant foods provide more than 80% of vitamin A intake for people in developing countries. Therefore, the conversion efficiency of β-carotene to vitamin A is important, as it determines the effectiveness of plant foods as sources of vitamin A in humans. The objective of this study was to determine the effect of plant food antioxidants such as α-tocopherol, γ-tocopherol, α-tocotrienol, γ-tocotrienol and total γ-oryzanol on the cleavage of β-carotene in vitro. Rat intestinal mucosa post mitochondrial fractions were incubated with β-carotene-rich extracts of kale and biofortified maize for an hour at 37°C. Rat intestinal mucosa post mitochondrial fractions were also incubated with β-carotene in the presence of either α-tocopherol, γ-tocopherol, α-tocotrienol, γ-tocotrienol or γ-oryzanol for 60 min at 37°C. The β-carotene cleavage products were extracted and analyzed by an HPLC equipped with a C18 column at 340nm and 450nm. When β-carotene alone was incubated without intestinal mucosa homogenate (control), no cleavage products were detected. When β-carotene alone was incubated with intestinal mucosa homogenate, β-apo-13-carotenone, β-apo-14-carotenal, retinal, retinol and retinoic acid were formed. However, incubation of β-carotene with either α-tocopherol, γ-tocopherol or α-tocotrienol resulted in a 10 fold inhibition of β-apo-14-carotenal and β-apo-13-carotenone formation. Antioxidant rich biofortified maize extract incubated with postmitochondrial fraction produced less β-apo-13-carotenone compared to the kale extract. These results suggest that antioxidants inhibit the cleavage of β-carotene and the formation of excentric cleavage products (β-apo-13-carotenone, β-apo-14-carotenal).