Relevant bibliographies by topics / PLAA

Academic literature on the topic 'PLAA'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "PLAA"

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Qu, Xiaoyan, Xiaowen Song, Nannan Zhang, Jinming Ma, and Honghua Ge. "The phospholipase A effector PlaA from Legionella pneumophila: expression, purification and crystallization." Acta Crystallographica Section F Structural Biology Communications 76, no. 3 (March 1, 2020): 138–44. http://dx.doi.org/10.1107/s2053230x20002149.

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Legionella pneumophila encodes an extracellular secreted phospholipase A named PlaA that is translocated by the type II secretion system. It plays an essential role in maintaining the integrity of Legionella-containing vacuoles in L. pneumophila pathogenesis. Here, it is shown that PlaA has a main lysophospholipase activity to hydrolyze fatty-acyl groups in lysophospholipids. Although it has a very low phospholipase A activity to catalyze the hydrolysis of fatty-acyl groups in phospholipids, PlaA can bind phospholipids such as 1,2-dipalmitoylphosphatidylcholine with a dissociation constant of 11.1 µM. Sequence-alignment analysis combined with activity assays revealed that PlaA contains a distinct substrate-binding site among the known structures of the phospholipase A family, implying that PlaA may present a novel mechanism for substrate recognition. Native PlaA and its selenomethionine (SeMet)-substituted form were purified and crystallized by vapour diffusion in hanging drops at 296 K. Diffraction data were collected to a resolution of 2.0 Å for native PlaA protein and to a resolution of 2.7 Å for SeMet-substituted PlaA protein. The crystals of native PlaA belonged to the monoclinic space group P21, while the crystals of SeMet-substituted PlaA belonged to the primitive orthorhombic space group P212121. Initial phases for PlaA were obtained from SeMet SAD data sets.
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Flieger, Antje, Birgid Neumeister, and Nicholas P. Cianciotto. "Characterization of the Gene Encoding the Major Secreted Lysophospholipase A of Legionella pneumophila and Its Role in Detoxification of Lysophosphatidylcholine." Infection and Immunity 70, no. 11 (November 2002): 6094–106. http://dx.doi.org/10.1128/iai.70.11.6094-6106.2002.

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ABSTRACT We previously showed that Legionella pneumophila secretes, via its type II secretion system, phospholipase A activities that are distinguished by their specificity for certain phospholipids. In this study, we identified and characterized plaA, a gene encoding a phospholipase A that cleaves fatty acids from lysophospholipids. The plaA gene encoded a 309-amino-acid protein (PlaA) which had homology to a group of lipolytic enzymes containing the catalytic signature GDSL. In Escherichia coli, the cloned gene conferred trypsin-resistant hydrolysis of lysophosphatidylcholine and lysophosphatidylglycerol. An L. pneumophila plaA mutant was generated by allelic exchange. Although the mutant grew normally in standard buffered yeast extract broth, its culture supernatants lost greater than 80% of their ability to release fatty acids from lysophosphatidylcholine and lysophosphatidylglycerol, implying that PlaA is the major secreted lysophospholipase A of L. pneumophila. The mutant's reduced lipolytic activity was confirmed by growth on egg yolk agar and thin layer chromatography and was complemented by reintroduction of an intact copy of plaA. Overexpression of plaA completely protected L. pneumophila from the toxic effects of lysophosphatidylcholine, suggesting a role for PlaA in bacterial detoxification of lysophospholipids. The plaA mutant grew like the wild type in U937 cell macrophages and Hartmannella vermiformis amoebae, indicating that PlaA is not essential for intracellular infection of L. pneumophila. In the course of characterizing plaA, we discovered that wild-type legionellae secrete a phospholipid cholesterol acyltransferase activity, highlighting the spectrum of lipolytic enzymes produced by L. pneumophila.
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Sun, Yuan, Xiang-Qi Li, Peyman Sahbaie, Xiao-You Shi, Wen-Wu Li, De-Yong Liang, and J. David Clark. "miR-203 Regulates Nociceptive Sensitization after Incision by Controlling Phospholipase A2 Activating Protein Expression." Anesthesiology 117, no. 3 (September 1, 2012): 626–38. http://dx.doi.org/10.1097/aln.0b013e31826571aa.

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Background After incision keratinocytes in the epidermis become activated to produce a range of pain-related mediators. microRNA 203 (miR-203) is known to be involved in keratinocyte growth, differentiation, and skin inflammation. We hypothesized that one or more of these mediators might be under the control of miR-203. Methods The expression of miR-203 and its target gene, phospholipase A2 activating protein (PLAA), were examined after hind paw incision in mice. We investigated the local effect of intraplantar PLAA peptide injection in normal mice and the effects of a selective secretory phospholipase A2 inhibitor (HK064) on PLAA or incision-induced mechanical allodynia. Last, we investigated the role of substance P signaling in regulating miR-203 and PLAA expression in vitro and in vivo. Results Levels of miR-203 were strongly down-regulated in keratinocytes after incision. Informatics-based approaches identified PLAA as a likely candidate for regulation by miR-203. PLAA caused mechanical allodynia and conditioned place aversion but not thermal sensitization. HK064 reduced mechanical allodynia after incision and after intraplantar injection of PLAA. Using preprotachykinin gene knockout mice or with neurokinin-1 selective antagonist LY303870 treatment, we observed that substance P-mediated signaling was also required for miR-203 and PLAA regulation after incision. Finally, using the rat epidermal keratinocyte cell line, we observed that a miR-203 mimic molecule could block the substance P-induced increase in PLAA expression observed under control conditions. Conclusions miR-203 may regulate expression of the novel nociceptive mediator PLAA after incision. Furthermore, the regulation of miR-203 and PLAA levels is reliant upon intact substance P signaling.
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Park, Jiyeon, Gyeong Tae Eom, Joon Young Oh, Ji Hyun Park, Sun Chang Kim, Jae Kwang Song, and Jung Hoon Ahn. "High-Level Production of Bacteriotoxic Phospholipase A1 in Bacterial Host Pseudomonas fluorescens via ABC Transporter-Mediated Secretion and Inducible Expression." Microorganisms 8, no. 2 (February 11, 2020): 239. http://dx.doi.org/10.3390/microorganisms8020239.

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Bacterial phospholipase A1 (PLA1) is used in various industrial fields because it can catalyze the hydrolysis, esterification, and transesterification of phospholipids to their functional derivatives. It also has a role in the degumming process of crude plant oils. However, bacterial expression of the foreign PLA1-encoding gene was generally hampered because intracellularly expressed PLA1 is inherently toxic and damages the phospholipid membrane. In this study, we report that secretion-based production of recombinant PlaA, a bacterial PLA1 gene, or co-expression of PlaS, an accessory gene, minimizes this harmful effect. We were able to achieve high-level PlaA production via secretion-based protein production. Here, TliD/TliE/TliF, an ABC transporter complex of Pseudomonas fluorescens SIK-W1, was used to secrete recombinant proteins to the extracellular medium. In order to control the protein expression with induction, a new strain of P. fluorescens, which had the lac operon repressor gene lacI, was constructed and named ZYAI strain. The bacteriotoxic PlaA protein was successfully produced in a bacterial host, with help from ABC transporter-mediated secretion, induction-controlled protein expression, and fermentation. The final protein product is capable of degumming oil efficiently, signifying its application potential.
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Klaassen, Curtis. "In Memorium: Gabriel L. Plaa." Toxicological Sciences 113, no. 2 (December 7, 2009): 279–80. http://dx.doi.org/10.1093/toxsci/kfp295.

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Manch-Citron, Jean N., Anjana Dey, John B. Ewell, and Nga Y. Nguyen. "Mutant analysis of Prevotella sp. plaA-lacZ fusion protein expression in Escherichia coli: support for an essential role of the stem-loop." Canadian Journal of Microbiology 45, no. 2 (February 1, 1999): 153–61. http://dx.doi.org/10.1139/w98-232.

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This study investigated the involvement of RNA folding in the synthesis of a fusion protein with beta-galactosidase activity. The coding gap region of the Prevotella loescheii adhesin gene plaA was fused in-frame with the Escherichia coli lacZ gene on plasmid pSK105. N-Terminal sequencing of the expressed plaA-lacZ protein indicated that it resulted from translational initiation at a fortuitous ribosomal-binding site within the plaA sequence at nt 570. Specific mutations were introduced in the stem-loop region that precedes the gap sequence. Analysis of stem-loop mutants, together with the introduction of compensatory mutations that restored activity, supports a requirement for stem-loop formation within the plaA sequence preceding the translational initiation site. A mutation reducing the predicted size of the loop, but preserving the stem structure, inactivated fusion protein synthesis. A suppressor mutation predicted to restore the size of the loop restored efficient fusion protein synthesis. In addition, the sequence preceding the translational start site of the plaA-lacZ fusion has several similarities to sequences that function as translational enhancers in prokaryotes. These include a stem-loop structure, an A-U rich region preceding the initiation codon, and a region of homology to 16S rRNA.Key words: site-directed mutagenesis, stem-loop formation, fusion protein, translational initiation, translational enhancer.
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Mullally, James E., Tatiana Chernova, and Keith D. Wilkinson. "Doa1 Is a Cdc48 Adapter That Possesses a Novel Ubiquitin Binding Domain." Molecular and Cellular Biology 26, no. 3 (February 1, 2006): 822–30. http://dx.doi.org/10.1128/mcb.26.3.822-830.2006.

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ABSTRACT Cdc48 (p97/VCP) is an AAA-ATPase molecular chaperone whose cellular functions are facilitated by its interaction with ubiquitin binding cofactors (e.g., Npl4-Ufd1 and Shp1). Several studies have shown that Saccharomyces cerevisiae Doa1 (Ufd3/Zzz4) and its mammalian homologue, PLAA, interact with Cdc48. However, the function of this interaction has not been determined, nor has a physiological link between these proteins been demonstrated. Herein, we demonstrate that Cdc48 interacts directly with the C-terminal PUL domain of Doa1. We find that Doa1 possesses a novel ubiquitin binding domain (we propose the name PFU domain, for PLAA family ubiquitin binding domain), which appears to be necessary for Doa1 function. Our data suggest that the PUL and PFU domains of Doa1 promote the formation of a Doa1-Cdc48-ubiquitin ternary complex, potentially allowing for the recruitment of ubiquitinated proteins to Cdc48. DOA1 and CDC48 mutations are epistatic, suggesting that their interaction is physiologically relevant. Lastly, we provide evidence of functional conservation within the PLAA family by showing that a human-yeast chimera binds to ubiquitin and complements doa1Δ phenotypes in yeast. Combined, our data suggest that Doa1 plays a physiological role as a ubiquitin binding cofactor of Cdc48 and that human PLAA may play an analogous role via its interaction with p97/VCP.
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Akutsu-Shigeno, Yukie, Teerawat Teeraphatpornchai, Kamonluck Teamtisong, Nobuhiko Nomura, Hiroo Uchiyama, Tadaatsu Nakahara, and Toshiaki Nakajima-Kambe. "Cloning and Sequencing of a Poly(dl-Lactic Acid) Depolymerase Gene from Paenibacillus amylolyticus Strain TB-13 and Its Functional Expression in Escherichia coli." Applied and Environmental Microbiology 69, no. 5 (May 2003): 2498–504. http://dx.doi.org/10.1128/aem.69.5.2498-2504.2003.

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ABSTRACT The gene encoding a poly(dl-lactic acid) (PLA) depolymerase from Paenibacillus amylolyticus strain TB-13 was cloned and overexpressed in Escherichia coli. The purified recombinant PLA depolymerase, PlaA, exhibited degradation activities toward various biodegradable polyesters, such as poly(butylene succinate), poly(butylene succinate-co-adipate), poly(ethylene succinate), and poly(ε-caprolactone), as well as PLA. The monomeric lactic acid was detected as the degradation product of PLA. The substrate specificity toward triglycerides and p-nitrophenyl esters indicated that PlaA is a type of lipase. The gene encoded 201 amino acid residues, including the conserved pentapeptide Ala-His-Ser-Met-Gly, present in the lipases of mesophilic Bacillus species. The identity of the amino acid sequence of PlaA with Bacillus lipases was no more than 45 to 50%, and some of its properties were different from those of these lipases.
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Phupaboon, Srisan, Sutrita Punyauppa-path, Papatchaya Kontongdee, Weera Piyatheerawong, and Sirinda Yunchalard. "Development and enhancement of antioxidant peptides from spontaneous plaa-som fermentation co-stimulated with Chiangrai Phulae pineapple enzymatic reaction." International Food Research Journal 29, no. 2 (April 1, 2022): 406–15. http://dx.doi.org/10.47836/ifrj.29.2.18.

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The present work aimed to search for a released peptide from proteolytic action on a silver barb fish muscular protein that confers health benefit through antioxidation activity. Changes in the physicochemical, microbiological, and protein characteristics of plaa-som samples during eight days of both spontaneous traditional fermentation (Batch 1; B1) and spontaneous fermentation with the addition of pineapple (Batch 2; B2) were determined. Results showed a correlation between an increase in the total acidity and bacterial counts with the length of fermentation duration, where the pH gradually decreased at the end of fermentation. Protein hydrolysis during fermentation was indicated by an increase in the amount of TCA-soluble peptide contents that peaked on day 5 (D5) in both batches (B1D5 and B2D5), which displayed their highest DPPH radical-scavenging inhibition of plaa-som protein hydrolysates (PSPHs). Twelve peptide fractions of the best PSPH were separated by ultrafiltration using molecular weight cut off (MWCO) at 3 and 10 kDa, and they were also purified by size exclusion chromatography. Results demonstrated that stronger peptides B2D5 - 3 kDa - F1 and B1D5 - 10 kDa - F1 were arranged in 12 peptides, which exhibited the highest reducing power, more than their radical-scavenging inhibition (p < 0.05). Therefore, it was concluded that both peptides obtained from PSPH have released antioxidative peptides that could be beneficial towards consumer's health, particularly the spontaneous plaa-som fermented with the addition of pineapple.
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Qiu, Liyan, Natasha Pashkova, John R. Walker, Stanley Winistorfer, Abdellah Allali-Hassani, Masato Akutsu, Robert Piper, and Sirano Dhe-Paganon. "Structure and Function of the PLAA/Ufd3-p97/Cdc48 Complex." Journal of Biological Chemistry 285, no. 1 (November 2, 2009): 365–72. http://dx.doi.org/10.1074/jbc.m109.044685.

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Dissertations / Theses on the topic "PLAA"

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Hiller, Miriam Verfasser], Antje [Akademischer Betreuer] Flieger, and Michael [Akademischer Betreuer] [Steinert. "Characterization of the Legionella pneumophila GDSL hydrolases PlaA and PlaD regarding enzymatic activity and modulation of host cell signaling / Miriam Hiller ; Antje Flieger, Michael Steinert." Braunschweig : Technische Universität Braunschweig, 2021. http://d-nb.info/1227577648/34.

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Doroudi, Maryam. "Essential roles of Pdia3/PLAA receptor complex and CaMKII IN 1α,25(OH)₂D₃ and Wnt5a calcium-dependent signaling pathways in osteoblasts and chondrocytes." Diss., Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/53427.

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The vitamin D metabolite 1,25-dihydroxyvitamin D3 [1α,25(OH)2D3] plays an important role in the regulation of musculoskeletal growth and differentiation. 1α,25(OH)2D3 mediates its effects on cells, including chondrocytes and osteoblasts, through the classical nuclear 1α,25(OH)2D3 receptor. Additionally, recent evidence indicates that several cellular responses to 1α,25(OH)2D3 are mediated via a rapid, calcium-dependent membrane-mediated pathway. These actions of 1α,25(OH)2D3 can be blocked by antibodies to protein-disulfide isomerase family A, member 3 (Pdia3), indicating that it is part of the receptor complex; however, the pathway which is activated by this receptor is not fully understood. The overall goal of this thesis was to examine the roles of phospholipase A2 activating protein (PLAA) and calcium calmodulin-dependent kinase II (CaMKII) in 1α,25(OH)2D3 rapid membrane-mediated signaling. We further investigated the interaction between two pathways regulating growth plate cartilage and endochondral bone formation, 1α,25(OH)2D3 and Wnt5a, at the receptor complex level. Results indicated that PLAA was required for mediating 1α,25(OH)2D3 signal from Pdia3. Furthermore, CaM and CaMKII were identified as mediators of 1α,25(OH)2D3-stimulated PLAA-dependent activation of cPLA2 and PKCα, and downstream biological effects. Wnt5a and 1α,25(OH)2D3 are important regulators of endochondral bone formation. This study demonstrated that 1α,25(OH)2D3 and Wnt5a mediate their effects via similar receptor components in osteoblasts and chondrocytes suggesting that these pathways may interact.
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Huayta, Pinedo Tessy Romina, Canal Christian Jaime Oliva, Reyes Janet Estela Robles, and Suarez Geraldine Winiber Vilchez. "Plan de Negocios Play Day S.A.C." Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2017. http://hdl.handle.net/10757/623046.

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El objetivo de crear Play Day es ofrecer un valor agregado a los padres de familia que buscan pasar un momento de diversión con sus hijos en las fechas más importantes de celebración. Es así que Play Day “Reconéctate Jugando”, ofrece el servicio de alquiler de videojuegos para amenizar fiestas infantiles y diversos eventos con un concepto innovador que busca reconectar a todos los invitados con la participación de un animador que será el encargado de fomentar la participación e integración de todos los asistentes, motivo por el cual consideramos que nuestra propuesta es innovadora debido a que no existe en el mercado peruano un servicio que promueva esos beneficios de reconexión y además que integre en su equipo a un animador para cada evento; por tanto, la estrategia que vamos utilizar será la diferenciación.
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Salinas, Sedano Carlos Teofilo 1960. "Formação de gelo em placa plana." [s.n.], 1996. http://repositorio.unicamp.br/jspui/handle/REPOSIP/263314.

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Orientador: Kamal A. R. Ismail
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Mecanica
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Resumo: Este trabalho modela o processo de formação de gelo sobre a superfície de uma placa ma quando é exposta a um fluxo de ar úmido. O modelo matemático utilizado é uma formulação unidimensional e transiente de fluxo e transferência de calor e massa, baseado na técnica de media volumétrica local. Utilizando este modelo pode-se predizer a densidade e o crescimento da espessura da camada porosa de gelo, assim como também a temperatura da superfície da camada porosa de gelo. Outras propriedades como a temperatura, a taxa de deposição de massa e a fração volumétrica das fases no interior da camada porosa de gelo também são avaliadas. Dados experimentais e numéricos disponíveis foram usadas para validar o modelo. Também foram realizados experimentos numéricos para escolher os melhores valores iniciais de difusividade e raio inicial do cristal de gelo, assim como o parâmetro de difusão superficial Fs. Utiliza-se finalmente este modelo para predizer a formação de gelo em placas paralela
Abstract: This paper models the frost formation process on a cold flat surface exposed to the flow of warm moist air. The model is a one dimensional transient formulation of flow, heat and mass transfer based upon the local volume averaging technique. This model enables predicting the ftost temperature, density and thickness distribution along the plate as well as the void fraction. Available experimental data was used to validate the model. Also numerical experiments were realized to choose the best initial values of the diffusivity and initial radius of the ice crystals and the frost diffusion parameter Fs. The model is also enables predicting a frost formation on paralleles flat plates
Mestrado
Termica e Fluidos
Mestre em Engenharia Mecânica
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Acuña, Bugueño Constanza, González Diego Espinosa, and Numhauser Benjamín Puelma. "Plan de negocios : restructuración "Playa Jamaica." Tesis, Universidad de Chile, 2016. http://repositorio.uchile.cl/handle/2250/139620.

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Seminario para optar al título de Ingeniero Comercial, Mención Administración
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Playa Jamaica es una tienda ubicada en la ciudad de La Serena, en un sector estratégico de la avenida del Mar, en concesión marítima otorgada por el Estado Chileno a partir de enero de 2013 y hasta 2020, renovable. Posee 400 metros cuadrados, donde se encuentra construida dos terrazas, un quiosco, servicios higiénicos y una cocina. Actualmente se atiende a la comunidad local y turistas, ofreciendo variedades de empanadas y jugos naturales, snacks, helados, cigarros, galletas y confites. También se ofrece servicios higiénicos para los veraneantes a un costo de $500. En el verano, debido a la masiva llegada de turistas a la cuarta región, Playa Jamaica tiene su mayor época de ingresos. Hoy en día durante el verano se pone música para los clientes que visitan el local, en un ambiente agradable y familiar, atendido por sus dueños. Creemos que como funciona actualmente el local, no está aprovechando la gran oportunidad que le genera el lugar donde está ubicado. Existe una demanda muy grande por distintos productos y servicios durante la época estival, la cual necesita tener un equilibrio con la oferta presentada, la que actualmente no cumple con lo necesario. Día a día, la vida sana y el deporte gana más adeptos dentro de Chile, con lo cual es un motivo para realizar una restructuración completa, ofreciendo a los ya típicos productos de empanadas y jugos, alternativas saludables de comida, junto con un conjunto de actividades de deporte y recreación que podamos ofrecer a los clientes y veraneantes. La reestructuración incluye una remodelación completa de como actualmente está dispuesto el local. Apertura de nuevas terrazas, una distinta organización y una oferta de una gran variedad de productos saludables relacionados a la vida sana y el deporte. Dentro de los puntos importantes a destacar, se quiere aprovechar el lugar donde se encuentra ubicado el local, un sector muy concurrido de la ciudad, para la venta de publicidad y apoyo de grandes empresas como auspiciadoras de las actividades deportivas que presentaremos
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Comunelo, Rossano Renir. "Convecção natural em placa plana vertical." Florianópolis, SC, 2007. http://repositorio.ufsc.br/xmlui/handle/123456789/90300.

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Dissertação (mestrado) - Univesidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-graduação em Engenharia Mecânica
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Trata-se de um estudo numérico-experimental de convecção natural laminar de uma placa plana vertical isotérmica em meio ao ar quiescente avaliando a influência de superfícies vizinhas. O objetivo é estimar o coeficiente de troca de calor por convecção em função da aproximação de superfícies (lateral e inferior), e como essa aproximação pode ser descrita em termos de parâmetros adimensionais, tais como RaL e NuL. A placa é aquecida por uma resistência térmica mantendo-se sua temperatura superficial constante. Na bancada experimental foi utilizada a tecnologia de transdutores de fluxo de calor à gradiente tangencial para a leitura do fluxo de calor, sendo obtido o coeficiente de transferência de calor por convecção, hexp. Para a solução numérica foi utilizado um código comercial em CFD, ANSYS-CFX, para a simulação do caso proposto, efetuando-se dessa forma comparações entre as duas soluções. O efeito da espessura da borda de base reta é estudado computacionalmente apontando assim qual influência desta no escoamento, na troca de calor e nos parâmetros adimensionais característicos desse tipo de escoamento. A influência da altura da placa vertical aquecida é avaliada numericamente e experimentalmente. Os resultados mostram que o coeficiente de troca de calor por convecção natural é influenciado por vários fatores sendo o mais importante a proximidade com uma parede adiabática vertical posterior à face aquecida e sendo potencializada pela presença das duas placas planas adiabáticas. As condições de contorno influenciam no campo de velocidade e, portanto, no coeficiente de troca de calor. This is about an numerical and experimental study of laminar natural convection of an isothermal vertical plain plate in way to air evaluating the influence of neighboring surfaces. The objective to guess the coefficient of exchange of heat for convection in function of the approach of surfaces (lateral and inferior), and as this approach can be described in terms of dimensionless parameters, such as RaL and NuL. The warm plate for a thermal resistance remaining its constant superficial temperature. In the experimental group of benches the technology of transducers of flow of heat at tangential gradient for the reading of the flow of heat, being gotten the coefficient of transference of heat for convection was used, hexp. For the numerical solution a commercial code in CFD was used, ANSYS-CFX, for the simulation of the considered case, effecting of this form comparisons between the two solutions. The effect of the thickness of the base edge straight line is studied computational thus pointing which influence of this in the draining, the exchange of heat and characteristic the dimensionless parameters of this type of draining. The influence of the height of the warm vertical plate is evaluated numericamente and experimentally. The results show that the coefficient of exchange of heat for natural convection is influenced by some factors having been the most important proximity with a adiabatic wall vertical line subsequent to the warm face and being powerfull for the presence of the two adiabatics plain plates. The contour conditions influence in the field of speed and, therefore, in the coefficient of heat exchange.
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Alburez, Samayoa Luis Alejandro. "Plan de Negocios de Plaza Comercial “María”." Tesis, Universidad de Chile, 2011. http://www.repositorio.uchile.cl/handle/2250/102563.

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McNeil, Sallman Cyndi. "Play therapy : an overview and marketing plan." Manhattan, Kan. : Kansas State University, 2007. http://hdl.handle.net/2097/363.

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Lavagna, Luis Guillermo Moscoso. "Aerodinâmica da placa plana em movimento não-permanente." Instituto Tecnológico de Aeronáutica, 1992. http://www.bd.bibl.ita.br/tde_busca/arquivo.php?codArquivo=1797.

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Considere-se uma placa plana, imersa num fluido que escoa, a qual executa um movimento não-permanente qualquer. O objetivo básico deste trabalho é calculas numericamente o campo de escoamento em torno da placa e, dessa forma, obter-se os coeficientes aerodinâmicos de sustentação e momento de arfagem. As principais hipóteses simplificadoras são: fluido não-viscoso, escoamento incompressível e irrotacional. O modelo que daí resulta permite a simulação da placa mediante uma distribuição de dipolos normais de intensidade uniforme. Para se obter o campo de velocidades lança-se mão do método dos painéis, sendo que o calculo dos coeficientes aerodinâmicos é feito utilizando-se a equação de Bernoulli linearizada. Os casos considerados para o movimento da placa são: mudança brusca do ângulo de incidência em relação a um escoamento uniforme (problema de Wagner), entrada paulatina numa rajada de canto vivo (problema de Kussner), entrada paulatina numa rajada de intensidade harmonicamente variável (problema de Sears) e movimento harmônico combinado de translação e rotação (problema de Theodorsen). O procedimento de calculo proposto é validado, para cada caso, via comparação com resultados analíticos encontrados na literatura.
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Santos, Marcelo Antonio dos. ""MGLTSmn" : aproximação angular "multigrid" em uma placa plana." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2005. http://hdl.handle.net/10183/6154.

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Neste trabalho, desenvolve-se um método “multigrid” para a aproximação angular da solução da equação de transporte de partículas em uma placa plana, baseado na formulação LTSN com dependência contínua na variável angular. Para tanto, aplica-se a formulação LTSN sobre o conjunto de equações SN para determinar o fluxo angular de partículas nas N direções discretas referentes a uma malha grossa (N pequeno) e em seguida, usando os fluxos conhecidos, aplica-se a formulação LTSN com dependência angular contínua, para avaliar o fluxo angular nas M direções discretas referentes a uma malha fina (M grande). São apresentadas simulações numéricas que ilustram a capacidade desse método, denotado por MGLTSMN , no que diz respeito à redução do esforço computacional na aproximação da solução para problemas que requerem elevadas ordens de quadratura e alto grau de anisotropia.
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Books on the topic "PLAA"

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Glasgow (Scotland). Parks and Recreation Department. Children's play development plan. Glasgow: Glasgow City Council, 1997.

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Highland Council (Scotland). Culture and Leisure Services. Corporate service plan =: Plana seirbheis. Inverness: Highland Council, 1998.

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A plan of action: A play. London: Samuel French, 2008.

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Highland Council (Scotland). Planning Service. Lochaber local plan =: Plana ionadail Lochabar. (Inverness: The Council), 1997.

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Kharal, Rāʼe Asad K̲h̲ān. Prinsis ainḍ plae boāʼe =: Princess & play boy. 5th ed. Lāhaur: Īnṭelījins Pablisharz, 2006.

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Alberta. Alberta Advanced Education and Technology. Advancing PLAR in Alberta: An action plan. Edmonton, AB: Alberta Advanced Education and Technology, 2008.

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Bray, Arthur. Plan today, play tomorrow: Achieving early financial freedom. 2nd ed. Don Mills, Ont: CCH Canadian, 1992.

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Plana Leasachaidh Canain: A development plan for gaelic. Inverness: Comunn na Gàidhlig, 1999.

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Leiva, María Ester. La conquista de Playa Bristol: Mar del Plata : 1936-2001. Mar del Plata: Centro de Estudios Históricos Arquitectónico Urbanos, Facultad de Arquitectura, Urbanismo y Diseño, Universidad Nacional de Mar del Plata, 2003.

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North Somerset (England). Planning and Environment Department. Waste local plam: Revised deposit plan, report on public consultation.. August 2000. Weston-super-Mare: North Somerset Council, 2000.

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Book chapters on the topic "PLAA"

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Liu, Huailin, and Jing Liu. "PlAA: Pixel-level Adversarial Attack on Attention for Deep Neural Network." In Lecture Notes in Computer Science, 611–23. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-15919-0_51.

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Kennedy, B. P., P. Vadas, and W. Pruzanski. "Secretory PLAA2-Deficient and Transgenic Mice in Phospholipase A2 Research." In Phospholipase A2, 65–71. Basel: KARGER, 1997. http://dx.doi.org/10.1159/000075487.

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Kletter, Raz. "The comprehensive plan for the Western Wall Plaza." In Archaeology, Heritage and Ethics in the Western Wall Plaza, Jerusalem, 120–48. First edition. | New York : Routledge, [2019] | Series: Copenhagen international seminar: Routledge, 2019. http://dx.doi.org/10.4324/9780429031311-6.

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James, Peet. "Plan, Plan, Plan." In Verification Plans, 1–10. Boston, MA: Springer US, 2004. http://dx.doi.org/10.1007/978-1-4615-0473-3_1.

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Larkin, Kevin, and Thomas Lowrie. "Play, Digital Play, and Play-Based Learning." In STEM Education in the Early Years, 69–84. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-2810-9_4.

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Konopinski-Klein, Nina. "Tagesplan – Plan plan dnia." In Polnisch-Deutsch für die Pflege zu Hause, 111–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-41808-2_8.

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Bujosevic, Dragan, and Ivan Radovanovic. "Attack Plan, Defense Plan." In The Fall of Milosevic, 30–35. New York: Palgrave Macmillan US, 2003. http://dx.doi.org/10.1057/9781403976772_7.

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Vanhoucke, Mario. "Plan." In The Data-Driven Project Manager, 5–27. Berkeley, CA: Apress, 2018. http://dx.doi.org/10.1007/978-1-4842-3498-3_2.

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Madej, Krystina. "Play." In Physical Play and Children’s Digital Games, 7–14. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-42875-8_2.

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Harteveld, Casper. "Play." In Triadic Game Design, 175–221. London: Springer London, 2011. http://dx.doi.org/10.1007/978-1-84996-157-8_5.

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Conference papers on the topic "PLAA"

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Hiniker, Alexis, Bongshin Lee, Kiley Sobel, and Eun Kyoung Choe. "Plan & Play." In IDC '17: Interaction Design and Children. New York, NY, USA: ACM, 2017. http://dx.doi.org/10.1145/3078072.3079752.

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"Crown Plaza Floor Plan." In 2005 Joint 30th International Conference on Infrared and Millimeter Waves and 13th International Conference on Terahertz Electronics. IEEE, 2005. http://dx.doi.org/10.1109/icimw.2005.1572374.

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Wang, Yi. "A New Plan for Toll Plaza." In Advances in Materials, Machinery, Electrical Engineering (AMMEE 2017). Paris, France: Atlantis Press, 2017. http://dx.doi.org/10.2991/ammee-17.2017.69.

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Lee, So-i., Joung Soon Park, Min Kyu Park, and Ja Kyung Koo. "Physical Property of Absorbable Suture PLLA I PLLA II PLGA PDO." In Green and Smart Technology 2015. Science & Engineering Research Support soCiety, 2015. http://dx.doi.org/10.14257/astl.2015.120.02.

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Jayme Rodrigues Teixeira Silva, Alvaro Francisco Santos Pivetta, Giannino Ponchio Camillo, Angelo Passaro, Marco Antonio Sala Minucci, Israel da Silveira Rego, Antonio Carlos Oliveira, and Paulo Gilberto de Paula Toro. "ESCOAMENTO SUPERSÔNICO NO BORDO DE ATAQUE DE UMA PLACA PLANA." In IX Congresso Nacional de Engenharia Mecânica. Rio de Janeiro, Brazil: ABCM Associação Brasileira de Engenharia e Ciências Mecânicas, 2016. http://dx.doi.org/10.20906/cps/con-2016-0793.

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Weigum, Shannon E., Melissa Sutton, Eugenia Barnes, Sarah Miller, and Tania Betancourt. "Targeting hepatocellular carcinoma with aptamer-functionalized PLGA/PLA-PEG nanoparticles." In SPIE NanoScience + Engineering, edited by Hooman Mohseni, Massoud H. Agahi, and Manijeh Razeghi. SPIE, 2014. http://dx.doi.org/10.1117/12.2062283.

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Vinci, F., J. De Jager, J. Schellekens, and C. Leo. "Play-Based Exploration and Development Plan for Geothermal Energy in the Netherlands." In EAGE 2020 Annual Conference & Exhibition Online. European Association of Geoscientists & Engineers, 2020. http://dx.doi.org/10.3997/2214-4609.202011071.

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Almeida Colmanetti, Alex Roger, Rubens Toledo, and Cristiano Tibiriçá. "Estudo experimental de formação de geada em placa plana sob convecção forçada." In VI Escola de Verão de Refrigeração. ABCM, 2021. http://dx.doi.org/10.26678/abcm.evr2021.evr21-0038.

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Vinci, F., J. De Jager, J. Schellekens, and C. Leo. "Play-Based Exploration and Development Plan for Geothermal Energy in the Netherlands." In 82nd EAGE Annual Conference & Exhibition - Workshop Programme. European Association of Geoscientists & Engineers, 2021. http://dx.doi.org/10.3997/2214-4609.202012243.

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Paulo Silva de Almeida, Pedro, José Leandro Cardoso Rivera Vila, jhon goulart, Tallita Nunes, and Tiago Melo. "ESTUDO DA TRANSFERÊNCIA DE CALOR POR CONVECÇÃO EM UMA PLACA PLANA POR IMAGEAMENTO TÉRMICO." In XXVI Congresso Nacional de Estudantes de Engenharia Mecânica. ABCM, 2019. http://dx.doi.org/10.26678/abcm.creem2019.cre2019-0197.

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Reports on the topic "PLAA"

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D. Shanklin. Characterization Plan for Soils Around Drain Line PLA-100115. Office of Scientific and Technical Information (OSTI), May 2006. http://dx.doi.org/10.2172/909843.

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Robles, Edgar A. Haiti Pension System: Recommendations to Improve the Regulation. Inter-American Development Bank, March 2018. http://dx.doi.org/10.18235/0003598.

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This tenth document of the PLAC Network Technical Assistance Document Series, entitled “Haiti pension system - Recommendations to improve the regulation”, provides general recommendations for the regulatory framework of the pension system in Haiti and tries to identify broad key policy options to improve the performance of the pension system. The policies are directed to strengthen and harmonize the rules of governance for the Office Nationale d'Assurance-Vieillesse (ONA), which covers private salaried workers, and the Plan de Retraite de l'Administration Publique (PRAP), to increase their capacity to manage risks, facilitate supervision of pension funds, improve sustainability, adequacy and equity, and establish guidelines for investments policies.
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Romero Ramírez, Álvaro Fernando, and Felipe Eugenio Mora Parra. Plan estratégico y prospectivo del municipio de la Plata Huila como eje regional de desarrollo de turismo sostenible 2020 – 2030. Universidad Nacional Abierta y a Distancia, 2021. http://dx.doi.org/10.22490/ecacen.5439.

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El turismo en Colombia, es un sector importante en la economía; el departamento del Huila posee un potencial alto de desarrollo en este sector; este trabajo se realizó en el municipio de La Plata, en un ámbito de desarrollo turístico sostenible; entendiendo esto como el equilibrio entre el sector privado, publico, académico y la comunidad. El objetivo del presente trabajo fue estructurar el plan estratégico y prospectivo del municipio de La Plata Huila para convertirlo como eje regional de desarrollo en el turismo sostenible 2020 – 2030. Para esto se utilizaron los métodos de prospectiva estratégica planteados por Mojica (2010); se desarrolló el análisis del sector, a través de estudios bibliométricos, patentometría, vigilancia tecnológica, aplicaciones como Mic-Mac, Mactor y ábaco de Regniere. También se realizó la estructuración de variables estratégicas, el análisis morfológico de los escenarios, la postulación de estos escenarios en los ejes de Peter Schwartz y la determinación del grado de gobernabilidad de las acciones a desarrollar. Los resultados mostraron que el desarrollo del sector turístico en el mundo, ha tenido como eje fundamental el turismo sostenible integrado con el ámbito rural. El escenario “La Plata Ciudad Región” fue elegido como reto, y en este se aprovecharon los potenciales del turismo rural y cultural, permitiendo que los turistas hicieran una inmersión en las actividades y los ambientes tradicionales. Además, presenta alianzas entre el estado y la empresa privada para potencializar estos cambios; en este escenario el turista disfruta de un parque automotor acondicionado, pensando en la comodidad y la seguridad.
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Mitchell, Leah, and Connie Lee. NTESS Retirement Income Plan Summary Plan Description. Office of Scientific and Technical Information (OSTI), July 2020. http://dx.doi.org/10.2172/1643983.

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Chadwick, Mark Benjamin. ALDX Mission Assurance Plan and Quality Plan. Office of Scientific and Technical Information (OSTI), November 2019. http://dx.doi.org/10.2172/1574733.

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Lee, Connie, and Theresa Mack. NTESS Retirement Income Plan, Summary Plan Description. Office of Scientific and Technical Information (OSTI), March 2022. http://dx.doi.org/10.2172/1861951.

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Dwyer, Stephen F. Test plan :. Office of Scientific and Technical Information (OSTI), May 2013. http://dx.doi.org/10.2172/1089866.

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Levine, Robert S., and William Rinkinen. Test plan:. Gaithersburg, MD: National Institute of Standards and Technology, 1989. http://dx.doi.org/10.6028/nist.ir.89-4034.

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Ressler, Sandy, and Susan Katz. Development plan:. Gaithersburg, MD: National Institute of Standards and Technology, 1990. http://dx.doi.org/10.6028/nist.ir.4413.

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Mitchell, Mary. Development plan:. Gaithersburg, MD: National Institute of Standards and Technology, 1990. http://dx.doi.org/10.6028/nist.ir.4417.

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