Academic literature on the topic 'Pinot Noir'

The aroma profile is a key component of Pinot noir wine quality, and this is influenced by the diversity, quantity, and typicity of volatile compounds present. Volatile concentrations are largely determined by the grape itself and by microbial communities that produce volatiles during fermentation, either from grape-derived precursors or as byproducts of secondary metabolism. The relative degree of aroma production from grape skins compared to the juice itself, and the impact on different yeasts on this production, has not been investigated for Pinot noir. The influence of fermentation media (Pinot noir juice or synthetic grape must (SGM), with and without inclusion of grape skins) and yeast choice (commercial Saccharomyces cerevisiae EC1118, a single vineyard mixed community (MSPC), or uninoculated) on aroma chemistry was determined by measuring 39 volatiles in finished wines using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC-MS). Fermentation medium clearly differentiated the volatile profile of wines with and without yeast, while differences between EC1118 and MSPC wines were only distinct for Pinot noir juice without skins. SGM with skins produced a similar aroma profile to Pinot noir with skins, suggesting that grape skins, and not the pulp, largely determine the aroma of Pinot noir wines.

DNA from 9 cultivars and 5 `Pinot noir' clones were isolated with either the Delaporta or cTAB methods Twenty five 32P label led cloned probes were constructed with the pUC18 plasmid and Hind-III digested `Pinot noir' DNA. Standard methods of isolation and labelling were used. The probes were tested for efficacy of `fingerprinting' the 14 selections. rDNA and cloroplast a/h binding protein probes were also tested. The non-specific probes were not found to be useful as they bound to an excess number of sites and could not be removed from the southern blots, rendering them useless for further analysis. Grape specific probes bound at multiple sites, indicating that multiple fragments were incorporated into the plasmid vectors during library construction. With the greater variability observable with these multi locus probes, significant polymorphism was observed between cultivars, including `Cabernet sauvignon' and `Pinot noir' which were not distinguishable with GPI or PGM isozymes. Variability between clones of `Pinot noir' was observed with several probes, indicating that these selections are different. No variability had been observed at isozyme loci of the `Pinot noir' clones

Volatile compounds are responsible for driving the aroma of wine. Because of their low perception thresholds, norisoprenoids may play an important role in wine aroma. Studies have shown that β-damascenone may act as an aroma enhancing compound. However, the direct impact on wine aroma is unclear. Our study examined the direct impact of β-ionone and β-damascenone on the aroma sensory perception of Pinot noir wines. Triangle tests were used to determine if assessors could distinguish between wines with varying concentrations of β-ionone and β-damascenone in three different Pinot noir wine matrixes. Descriptive analysis was performed on these treatments, perceived as different in triangle tests. Results show that β-ionone acts as a significant contributor to aromas in Pinot noir wine, as individuals could differentiate both the low and high concentration wines from the control. How β-ionone impacted wine aroma depends on the wine matrix, as different aroma descriptors were affected based on the model wine used, resulting in floral, red berry or dark berry aromas. The effect of β-damascenone on Pinot noir aroma was less clear, as perception seems to be heavily influenced by wine matrix composition. This study contributes to our understanding of the complex chemical causation of fruity aromas in Pinot noir wine.

Pinot noir is a grape variety with thin grape skin, which means the extraction of colour and polyphenols is more challenging than other red grape varieties. The aim of this study was to investigate the impact of protein removal by adding bentonite prior to fermentation on Pinot noir wine composition. Four treatments were conducted, including the control without bentonite addition and Pinot noir wines produced with the addition of three different types of bentonites before cold soaking. The juice and wine samples were analysed for pathogenesis-related proteins, tannin, wine colour parameters, and aroma composition. The results showed that bentonite addition at 0.5 g/L had little impact on tannin and aroma compounds but more impact on wine colour, especially significantly higher level of SO2 resistant pigments observed in Na bentonite addition treatment. This study indicates the potential use of bentonite to modulate the Pinot noir juice composition that may facilitate the extraction of colour components from grape into juice, which plays an important role in colour stabilization in finished wine.

Foliar and drench applications of the plant growth regulator abscisic acid (ABA) were applied to 1-year-old potted Vitis vinifera ‘Pinot Noir’ and ‘Cabernet Sauvignon’ vines infected with Xylella fastidiosa, the bacterial pathogen that causes Pierce's disease (PD). A naturally occurring ABA and a synthetic ABA were applied, and both materials showed some effectiveness at increasing curing rates of PD-affected grapevines. Pinot Noir grapevines treated with the drench ABA treatments had significantly greater disease curing effects than the unsprayed control plants. It has been shown that plant phenolics have antimicrobial properties, and we found a positive correlation between effective ABA treatments and the total phenolic compound content of xylem sap extracted from Pinot Noir vines.

High temperature adversely affects photosynthetic rates and thylakoid activities in many species, but photosynthesis response to heat stress is not well defined in grapes (Vitis L.). Genotypes within species respond differently to high temperatures, indicating a genetic variability for the trait. The objective of this study was to determine the physiological responses of two grape species to high temperature, at the whole-plant level and at the cellular level. Gas exchange, relative chlorophyll content, and chlorophyll fluorescence of intact leaves and thermostability of extracted thylakoids of the American (V. aestivalis Michx.) `Cynthiana' and European (V. vinifera L.) `Semillon', `Pinot Noir', `Chardonnay', and `Cabernet Sauvignon' wine grapes were evaluated. One-year-old vines were placed in controlled environmental chamber held at 20/15, 30/25, or 40/35 °C day/night for 4 weeks. Net CO2 assimilation (A) rate, stomatal conductance (gs), transpiration (E) rate, chlorophyll content, and chlorophyll fluorescence of intact leaves were measured at weekly intervals. Chlorophyll fluorescence of thylakoids extracted from V. aestivalis `Cynthiana' and V. vinifera `Pinot Noir' subjected to temperatures ranging from 20 to 50 °C was measured. Optimal temperatures for photosynthesis were 20/15 °C for `Cynthiana' and `Semillon' and 30/25 °C for the other three V. vinifera cultivars. The A, gs, E, chlorophyll content, and chlorophyll fluorescence values at 40/35 °C were lower in `Cynthiana' than `Pinot Noir'. In general, reduction of A coincided with decline in gs in `Cynthiana', whereas no strong relationship between A and gs was observed in V. vinifera cultivars. Variable chlorophyll fluorescence (Fv) and the quantum efficiency of photosystem II (Fv/Fm) of intact leaves for all the cultivars decreased at 40/35 °C, with severe decline in `Cynthiana' and `Cabernet Sauvignon,' moderate decline in `Semillon' and `Chardonnay', and slight decline in `Pinot Noir'. A distinct effect of high temperature on Fv and Fv/Fm of `Cynthiana' was exerted after 2 weeks of exposure. Prolonged-exposure to 40/35 °C led to 78% decrease in Fv/Fm in `Cynthiana', compared with 8% decrease in `Pinot Noir'. In general, Fv and Fv/Fm of extracted thylakoids declined as temperature increased, with more decline in `Cynthiana' than in `Pinot Noir'. Based on A rates and Fv/Fm ratios, results showed that `Cynthiana' has lower optimal temperature for photosynthesis (20/15 °C) than `Pinot Noir' (30/25 °C). Chlorophyll fluorescence responses of intact leaves and extracted thylakoids to high temperatures indicate that `Pinot Noir' possess higher photosynthetic activity than `Cynthiana'. Results of this work could be used in selection programs for the development of heat resistant cultivars in the warmest regions.

Microoxygenation (MOX) is used to improve wine colour and sensory quality; however, limited information is available for Pinot noir wines and wines with different initial phenolic content. In this study, MOX was applied to two Pinot noir wines, with either a low or a high phenolic content, at two doses (0.50 and 2.11 mg/L/day) for 14 days. With the sterile filtration applied, acetaldehyde formation during MOX was very low, supporting the influence of yeast on acetaldehyde production during MOX. The MOX dosage rate did not significantly affect colour development, while the Pinot noir wine with higher phenolics benefited more from MOX, significantly increasing colour intensity and SO2 resistant (polymeric) pigments. However, these changes did not guarantee colour stability, as a final SO2 addition (100 mg/L) largely erased the improvement to colour in all wines. This could be due to the lower acetaldehyde formation, thus less ethyl-bridged stable pigments resistant to SO2 bleaching. MOX also decreased the flavan-3-ols and anthocyanin monomers, which differed between the two Pinot noir wines, reflecting the initial phenolic content. Lastly, MOX generally increased the measured tannin concentration and affected the proportion of tannin subunits, with a decrease in tannin mass conversion and proportion of (-)-epigallocatechin extension units. Some of these changes in phenolic compounds could potentially increase astringency, suggesting that MOX should be applied to Pinot noir and other low phenolic wines with caution.

Critical levels (CL) of available potassium (K) in soils and leaves and maximum technical efficiency (MTE) doses are relevant information to define the best K fertilization strategies. The aim of this study was to determine reference values of K in leaves and soil, using Bayesian analysis, in order to maximize grape yield and must quality of ‘Chardonnay’ and ‘Pinot Noir’ grown in a subtropical climate. The vines were subjected to applications of 0, 20, 40, 60 and 80 kg K2O ha−1 year−1. The CL and MTE doses of K, in leaves and soils, were determined using Bayesian hierarchical models. The range of probability of the occurrence of CL in leaves was 15.8 to 18.9 g K kg−1 in ‘Chardonnay’ and 16.8 to 19.1 g K kg−1 in ‘Pinot Noir’ at flowering. Moreover, the range was 15.3 to 18.1 g K kg−1 in ‘Chardonnay’ and 16.1 to 18.2 g K kg−1 in ‘Pinot Noir’ at veraison. In the soil, the CL of available K for ‘Chardonnay’ was 27.4 mg K kg−1 and for ‘Pinot Noir’ it was 23.2 mg K kg−1. The increase of K in leaves and soil increased the TSS, while the TTA decreased in both cultivars after sufficiency rate. The MTE was estimated in ‘Pinot Noir’. The proposition of CL and/or MTE doses of K can help increase the efficiency of K fertilization in vineyards.

Two pre-fermentation treatments were investigated in Pinot noir (Vitis vinifera L.) wines. The effects of cold maceration and carbonic maceration on the wines' composition, colour parameters and sensory properties were examined. Cold maceration is a winemaking technique used to increase non-alcoholic extraction in Pinot noir winemaking prior to fermentation. It involves holding crushed grapes with approximately 100-150 mg l⁻¹ SO₂ at low temperatures and is thought to increase the colour, aroma and flavour of the resulting wines. Carbonic maceration uses whole bunches that have undergone anaerobic metabolism to produce characteristically fruity and spicy wines. Pre-fermentation cold maceration produces wines that are higher in titratable acidity and monomeric anthocyanin content, but lower in colour density, hue and polymeric pigments. Reducing the maceration temperature below 10°C has little effect. Carbonic maceration produces wines that are lower in titratable acidity, monomeric anthocyanin content, and colour density but are higher in colour hue and amount of polymeric pigments. Quantitative descriptive analysis was used to define the effects of these pre-fermentation maceration treatments on the sensory characteristics of the resulting wine. Trained panel members found that there were no discernable sensory differences in the compositional parameters despite measurable chemical differences. Investigation into the aroma and flavour characteristics of the wines found that carbonic maceration produces wines that were lower in berry aroma and higher in acetate or ester-type aromas than the control wines. These wines were considered to have specific raspberry, floral, sugar, cherry and chemical aromas. This chemical note was also observed in the flavour of the carbonic maceration wines. The temperature of the cold maceration process has no major effect on the aroma and flavour of the resulting wines. However, the 10°C maceration was higher in woody/tobacco aroma than the 4°C maceration, and the 10°C treatment was also higher in bitter flavour than all the other treatments. Cold maceration wines were found to have specific mixed berry, dried fruit and sweet-oxidised aroma characters, together with a blackberry flavour note.

La exposición de las plantas de vid a bajas temperaturas es uno de los factores que provocan la “coulure”. Este fenómeno involucra la aparición de perturbaciones a nivel de la fotosíntesis y el metabolismo del carbono de la planta. Con el fin de analizar el efecto de una noche fría sobre el contenido de los azúcares de reserva de las inflorescencias de Pinot noir, se expusieron ramas fructíferas de esta cepa de vid a temperaturas de 4°, 0° y -3°C durante una noche, para posteriormente determinar el contenido de glúcidos (glucosa, fructosa, sacarosa y almidón) de las inflorescencias. En paralelo, se realizaron observaciones microscópicas de las reservas amiláceas de las inflorescencias sometidas a una noche a -3°C. Los resultados de este estudio muestran que los contenidos en glucosa y fructosa de las inflorescencias fluctúan durante las primeras horas luego de un estrés de 4° y 0°C. Sin embargo, es sobre todo luego de una noche a -3°C que se pudo constatar una fuerte movilización y consumo de los azúcares de reserva contenidos en las inflorescencias estresadas. -- Palabras clave: Estrés frío, glúcidos, inflorescencia, vid, coulure.
-- Exposure to low temperatures is one of the main factors causing the coulure in grapevine, due to its effect on the plant’s photosynthesis and carbon metabolism. To test the effect of a chilling night on carbon reserves of inflorescences of Pinot Noir, fruiting cuttings that have undergone a low-temperature night at 4°, 0° and -3 ° C were used to determine the levels of carbohydrates (glucose, fructose, sucrose and starch) in the inflorescences. Microscopic observations of inflorescences after a -3 ° C night were performed. The levels of glucose and fructose fluctuated during the first hours after a 4 and 0 ° C night, but it is especially after a chilling night at -3 ° C that a strong mobilization and consumption of carbohydrates in the inflorescence were observed. -- Key words: Cold stress, carbohydrates, inflorescence, grapevine, coulure.
Tesis

Pinot noir has traditionally been fermented by native winery flora; however, the recent practice of single yeast strain inoculation may simplify wine aromas and flavours. Thus, a need exists for yeasts that create the complexity of naturally fermented wines and provide the consistency of commercial strains. It has been suggested that mixed Saccharomyces strains may be capable of this dual role. In this study, three novel Burgundian S. cerevisiae strains were characterized for enological equivalency against five industrial strains recommended for Pinot noir. The volatile compounds produced by these strains along with four Burgundian strain mixtures were quantified in Pinot noir wine to evaluate the hypothesis that mixed S. cerevisiae strains contribute to the complexity of naturally fermented wine. The Burgundian strains were enologically equivalent to the industrial strains in terms of killer phenotype, fermentation kinetics, production of ethanol, glycerol, and acetic acid, conversion of sugar to ethanol, ethanol tolerance, foam production, sulfur dioxide production, and compatibility with malolactic fermentation. The concentrations of most of the 25 compounds quantified in the headspace of Pinot noir wines fermented at 22°C and 27°C significantly differed among yeast strains and between temperatures. Principal component analysis revealed different patterns of volatile production among the industrial, individual Burgundian and mixed Burgundian yeast strains. Mixed Burgundian strains produced greater amounts of most of the higher alcohols than individual Burgundian strains. Additionally, individual and mixed Burgundian strains produced greater amounts of ethyl esters than most industrial strains, but did not differ from one another. In contrast, the pattern of acetate ester production differed between individual Burgundian and mixed Burgundian strains in a fermentation temperature-dependent manner. Cluster analysis revealed that differences in the patterns of volatile production among industrial, individual Burgundian and mixed Burgundian yeast strains extended over the complete volatile profile. Furthermore, cluster analysis of the averaged profiles showed greater overall similarity between the industrial and the individual Burgundian strains than the mixed Burgundian strains. Fermenting Pinot noir with mixed Burgundian yeast strains resulted in unique patterns of volatile production, which holds promise for mixed Saccharomyces products that yield wines of greater complexity.

A trial established in 2003 at Upper Moutere in Nelson, New Zealand, was used to evaluate the effect of mussel shells as reflective mulch on Vitis vinifera L. cv. Pinot noir vine performance and fruit and wine quality. Shell mulch had several effects on the environment and vine growth as well as grape and wine composition in the 2006/2007 season. Soil under mulch was cooler compared to un-mulched control, but buffered the extremes in temperatures. Fruiting zone temperature over shells was slightly higher during the day and cooler at night, showing no effect on mean hourly temperature. Shell mulch reflected greater amounts of UV-A, UV-B and PAR radiation into the fruiting zone. Shell reduced weed growth compared to control. Leaf petiole and blade samples showed higher amounts of calcium compared to control. Leaf SPAD values were higher in the shell treatment during veraison, previous and postharvest, but lower post budburst. While date of budburst was not affected by treatments, dates of flowering and veraison appeared to be slightly advanced over shells. Fruit set was similar between treatments and was considered poorer in shell bunches due to a larger population of seedless berries. Vine growth was not affected in terms of the number of nodes laid at pruning, flower cluster and shoot number pre shoot thinning, early shoot growth and lateral shoots development. Vigour was not increased by shells as demonstrated by pruning weights, canopy density and trunk circumferences being similar, though internode lengths in shell shoots were greater in 2007 and lower in 2006. Berry weights, bunch weights and vine yields were lower in shell than control, though greater berry numbers were recorded. There were slight differences between treatments in fruit and wine composition. Grape pH only varied in the middle of the sampling time, being higher the 2nd week and lower the 3rd week in shell grapes and TA was greater at harvest time. However, °Brix was only higher in shell grapes in the middle of the sampling period, being similar to control at veraison and harvest. Peduncle lignification was delayed at veraison as well as at harvest time. Shell must after crushing was greater in Brix but similar to control in pH and TA. Similarly, shell wines pre bottling showed higher alcohol and no differences for pH and TA. HPLC-DAD analyses of commercial-scale and microvin wines showed consistent differences of the individual flavonoid composition. Shell microvin wines were greater than control in quercetin and resveratrol. However, commercial shell wines were lower in epicatechin, gallic acid, resveratrol, and catechin than control. Leaf phenolic composition was also different between treatments. However, further analyses by HPLC-MS in wines as well as in leaves are necessary to identify individual compounds. Total anthocyanins and total phenolics were no different between treatments. Sensory analyses of microvin and commercial shell wines exhibited consistently lower levels of green and unripe tannins, and greater smoothness and complexity as well. Further analysis by GC-MS and HPLC-MS is warranted. Shell mulch improved sensory characteristics of the resulting wines.

ABSTRACT Influence of Crop Load on Fruit Composition Using Pinot Noir Grapes Patrick G. Phelan November 2009 The two seasons for this trial were conducted in 2002 and 2003 at Bien Nacido Vineyard in Santa Maria, California. The trial consisted of pinot noir wine grapes clone 2a, grown on 101-14 rootstock. There were eight treatments with a control consisting of 25 vines per treatment, and 50 vines for the control. The primary objective of this research experiment was to conduct different vine balance procedures and compare them to the amount of wine phenolics. The vines were altered with two procedures. First was crop yield; fruit thinning altered the treatments by providing four different crop loads and a control. The treatment levels were signified as one ton, two ton, three ton, four ton, and control. The second procedure was timing of fruit thinning. Four different crop loads were split in half giving rise to eight treatments and a control. With four of the treatments the fruit was dropped post-bloom, and the other four treatments were dropped at seventy five percent veraison. Equal amounts of early and late thinning were combined and made into wine which gave four different crop load wines and a control wine. This process was done in 2002 and repeated in 2003. Additionally, other vine growth parameters were analyzed to determine the role that crop load and timing of thinning had in their development. Seven variables were analyzed from prunings, clusters, juice, and wine samples. They consisted of berry size, cluster weight, titratable acidity, pH, total soluble solids, phenolic profile (consisting of eleven components), and shoot weight. The results indicated that the following were statistically significant: (1) titratable acidity was significantly different between the 2002 and 2003 growing seasons, (2) total soluble solids showed a significant difference between the early fruit thinning versus the late thinning, (3) berry size in 2002 showed a statistically significant effect of drop date on the mean caliper size, (4) in 2002 the berry size also showed a statistically significant interaction between drop level and drop date, (5) berry size in 2003 showed a statistically significant effect of drop level on the mean caliper size, (6) in 2003 there is also a statistically significant effect of drop date on the mean caliper size, (7) again in 2003 there is a statistically significant interaction between drop level and drop date, (8) cluster weights in 2002 showed a statistically significant relationship between drop level and average cluster weight, (9) in 2002 cluster weights also showed an effect of the time of fruit drop on the average cluster weight, (10) cluster weights in 2003 showed a statistically significant relationship between drop level and the average cluster weight, (11) pruning weights in 2002 showed a statistically significant relationship between drop level and average shoot weight, (12) pruning weights in 2003 showed a statistically significant relationship between drop level and average shoot weight and finally, (13) of the eleven phenolic components measured, five differed significantly by year but not across the five treatments. Information derived from this experiment suggests that the site is a high vigour location according to Robinson and Smart’s yield to pruning weight ratio. With this in mind we find that TA and pH were not a significant factor, but soluble solids (brix) ascertained a higher degree of brix with the late drop treatment. We conclude this to be an effect of both the high vigour site and more uniform fruit drop. In 2002 and 2003, we find a significant interaction between drop date and berry size with a late drop producing the smallest berries on average. Cluster weights varied between 2002 and 2003 and the results were inconclusive. Shoot weights along with harvest totals concluded a high vigour site. The one ton level in 2002 had a significantly higher average shoot weight than the three ton level (p<.001), four ton level (p=.006), and no fruit drop control (p<.001). There is also a significant relationship between drop time and average shoot weight in 2002 (p<.001), and 2003 (p=.049) with the late fruit drop being followed by a lower shoot weight. This significant relationship expressed that an early drop increased the shoot weight which contributed only more vigour to an already high vigour site. The ratio of yield to pruning weight for 2002 gave us a range of .39 to 1.78, and in 2003 the range was .32 to 1.36 concluding a high vigour site. The phenolic profile expressed a statistical difference by year, but was most likely caused by a varying fermentation temperature or different climatic conditions in 2002 and 2003.

La vitiviniculture est un secteur économique et culturel important en Bourgogne. L’actuel changement climatique soulève diverses questions notamment sur son impact sur les cultures. Dans cette thèse, l’idée est d’élaborer une méthodologie afin de répondre à la problématique : quels seront les impacts possibles des changements de températures sur la phénologie du Pinot noir en Bourgogne à l’horizon 2031-2048 ?L’évolution des températures en Bourgogne depuis 1961 est caractérisée par un saut positif de température à la fin des années 1980 suivi par une période où la température augmente d’environ 1,5°C.L’un des intérêts de cette thèse réside dans l’élaboration, en suivant une stratégie élaborée durant la thèse, d’une base de données spatialisée réalisée sur la période 1989-2009 afin d’estimer la capacité du modèle WRF à reproduire le climat bourguignon en désagrégeant des données climatiques de large échelle. Le modèle reproduit de façon satisfaisante le cycle saisonnier et la variabilité spatiale climatique globale aux biais près (froid sur les Tx et chauds sur les Tn).Pour régionaliser le changement climatique, WRF a été utilisé pour désagréger des données issues du scénario SRES/A2 sur les périodes 1970-1987 et 2031-2048. Après avoir été évalués et intercomparés trois modèles phénologiques utilisant les données de températures moyennes pour simuler les dates d’occurrence des stages phénologiques du Pinot noir, ont été appliqués sur ces désagrégations.L’impact de l’augmentation des températures à l’horizon 2031-2048 (SRES/A2), estimée à 1,35°C en moyenne, se caractérise par une précocité de la floraison d’au moins 7 jours et une précocité de la véraison d’au moins 15 jours. La durée interstade est également diminuée de l’ordre de 5 jours
The viticulture is an important economic and cultural sector in Burgundy. The current climate change raises a number of issues including its impact on crops. In this thesis, the idea is to develop a methodology to address the problem: what are the potential impacts of changes in temperature on the phenology of Pinot noir in Burgundy for years 2031-2048?The evolution of temperatures in Burgundy since 1961 is characterized by a positive temperature shift at the end of the 1980s followed by a period where the temperature increases of about 1.5 ° C.One of the interests of this thesis is to develop, following a strategy developed during the thesis, a spatial database conducted over the period 1989-2009 to estimate the ability of the WRF model to reproduce the climate Burgundy by disaggregating large scale data. The model reproduces satisfactorily the seasonal and spatial variability in global climate despite bias (cold on the Tx and hot on the Tn).To regionalize the climate change, WRF was used to disaggregate data from the scenario SRES/A2 on the periods 1970-1987 and 2031-2048. After being evaluated and inter-compared three phenological models, using average temperatures data to simulate the dates of occurrence of phenological stages of Pinot Noir, have been applied to these decompositions.The impact of warming temperatures on the horizon 2031-2048 (SRES/A2), estimated at 1.35 ° C on average, is characterized by an earlier flowering and veraison of about 7 and 15 days respectively. The interstadial duration is also reduced of about 5 days

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Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG)
O presente estudo teve como objetivo avaliar o efeito da cianamida hidrogenada e a fenologia de produ??o em cultivares Pinot Meunier e Pinot Noir no munic?pio de Diamantina/MG. Os experimentos foram conduzidos em propriedade comercial localizada em Diamantina/MG. O vinhedo foi instalado em 2005 com as cultivares Pinot Meunier e Pinot Noir, enxertadas sobre o porta-enxerto 1103 Pausen, implantado com espa?amento de 1 m entre plantas x 2,5 m entre fileiras. As plantas foram conduzidas com 2 hastes em esquema de espaldeira vertical e tr?s fios de arame. A poda foi realizada em 4 de setembro de 2008, deixando uma haste curta com duas gemas por espor?o. Para a caracteriza??o fenol?gica foram feitas observa??es visuais a cada dois dias da poda at? a colheita. As demandas t?rmicas foram calculadas a partir de temperaturas observadas na Esta??o Meteorol?gica de Diamantina/MG. Para as curvas de matura??o foi utilizado suco para avalia??o qu?mica dos teores de s?lidos sol?veis totais (SST), acidez total titul?vel (ATT) e rela??o SST/ATT. Para avaliar o efeito da aplica??o de cianamida hidrogenada na brota??o, in?cio de matura??o e nas caracter?sticas f?sico-qu?micas das cultivares Pinot Meunier e Pinot Noir cultivadas em Diamantina/MG, foram realizadas avalia??es das plantas a cada dois dias, quando foram aferidas as porcentagens de brota??o e porcentagens de bagas em in?cio de matura??o. Na colheita foi realizada a contagem do n?mero de cachos por planta e foram amostradas 15 bagas por parcela para as seguintes avalia??es f?sico-qu?micas das bagas: umidade, massa, di?metros longitudinal (DL) e transversal (DT), rela??o DL/DT, teor de s?lidos sol?veis totais (SST), acidez total titul?vel (ATT), rela??o STT/ATT, a??cares redutores, antocianinas, flavon?ides, compostos fen?licos e pH. Os resultados obtidos demonstram dura??o do per?odo fenol?gico para as cultivares Pinot Meunier e Pinot Noir de 145 e 155 dias, respectivamente, para a produ??o da safra de ver?o em Diamantina/MG, podendo considerar a variedade Pinot Meunier de ciclo precoce. A exig?ncia t?rmica necess?ria para a produ??o da Pinot Meunier da poda ? colheita foi de 1340,67 GD e da Pinot Noir de 1446,95 GD. A cultivar Pinot Noir apresentou maior teor de s?lidos sol?veis totais e acidez, sendo a maior rela??o SST/ATT da Pinot Meunier nas condi??es de Diamantina/MG. Foram evidenciados para o efeito da aplica??o da cianamida hidrogenada a antecipa??o do in?cio da matura??o em 17 dias para a Pinot Meunier, a maior porcentagem de cachos em in?cio de matura??o em 45,84% e 28,23% para ?Pinot Meunier? e ?Pinot Noir?, respectivamente, e o incremento de algumas caracter?sticas f?sico-qu?micas avaliadas, como acidez total titul?vel, rela??o SST/ATT, flavon?ides e taninos para a cultivar Pinot Meunier e s?lidos sol?veis totais, a??cares redutores e umidade das bagas para a cultivar Pinot Noir.
Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2010.
ABSTRACT This study aimed to evaluate the effect of hydrogenated cyanamide and phenology of production in Pinot Meunier and Pinot Noir cultivars in Diamantina/MG.?The experiments were conducted in commercial property located at Diamantina/MG.?The vineyard was established in 2005 with the Pinot Meunier and Pinot Noir cultivars, grafted on rootstock Pausen 1103, deployed with a spacement of 1 m between plants and 2.5 m between rows.?The plants were conducted with 2 rods in an arrangement of vertical cordon and three wires.?The pruning was performed on September 4, 2008 leaving a short stem with two buds per spur.?To characterize the phenology were made visually observations every two days from pruning to harvest.?The thermal demands were calculated from the observed temperatures at the meteorological station of Diamantina/MG.?For the maturation curves was used a juice to make chemical evaluation of total soluble solids (TSS), total titratable acidity (TTA) and the TSS/TTA ratio.?To evaluate the effect of hydrogenated cyanamide on sprouting, early maturation and the physicochemical characteristics of the Pinot Meunier and Pinot Noir cultivars grown in Diamantina/MG where the assessments of plants were made every two days, then there were measured the percentage of budding?and the percentage of berries in early maturation.?At harvest it was counted the number of clusters per plant and there were sampled 15 berries per plot for the following physicochemical evaluations of berries: humidity, mass, longitudinal diameter (DL) and transversal diameter (TD), LD/TD ratio content of?total soluble solids (TSS), total titratable acidity (TTA), TSS/ATT ratio, reducing sugars, anthocyanins, flavonoids, phenolic compounds and pH.?The results show that the duration of phenological period for the Pinot Noir and Pinot Meunier cultivars are from 145 to 155 days, respectively, for the production of summer crops in Diamantina/MG, which may consider the Pinot Meunier variety in early maturity.?The thermal requirement needed for the production of Pinot Meunier from pruning to harvest was 1340.67 GD and Pinot Noir presented 1446.95 GD.?Pinot Noir cultivar showed the highest content of total soluble solids and acidic, and also the highest TSS/TTA ratio of Pinot Meunier in the Diamantina/MG environment.?For the purpose of applying hydrogenated cyanamide there were evidenced the anticipation of early maturation in 17 days for Pinot Meunier, the highest percentage of early-ripening in 45.84% and 28.23% for Pinot Meunier and Pinot Noir, respectively and the increment of some physicochemical characteristics evaluated as total titratable acidity, TSS/TTA ratio, flavonoids and tannins for the Pinot Meunier cultivar and total soluble solid, reducing sugars and humidity from the berries to the Pinot Noir cultivar.

The effects of varying levels of sulphur dioxide (SO₂) on the cold maceration process was investigated with Pinot noir (Vitis vinifera L.) wine. The effects of these varying levels on the wines composition and colour parameters were examined. Cold maceration is a technique whereby grapes are crushed and placed at low temperatures (4 - lO°C) in the presence 50 - 150 mgL⁻¹ SO₂. This process is believed to provide a medium for the extraction of water soluble phenolic compounds, rather than the alcoholic extraction employed in normal fermentations. The extraction of these phenolic compounds was monitored from the juice through to six months of bottle age. The changes were measured using both Spectrophotometric and High Performance Liquid Chromatographic (HPLC) procedures. Cold maceration wines were found to be not significantly different to the control wine in all compositional parameters other than titrateable acidity which was found to be less than the control for all the cold maceration wines. The unsulphured cold maceration wine was not significantly different from the control wine in any of the spectral measurements except natural degree of ionisation, in which it was higher, and total phenolics, in which it was lower. These results indicate that the cold maceration process alone does not alter the extraction of phenolic compounds. The HPLC analysis of the wine confirmed the spectral results indicating that their were no significant differences in the levels of extraction of anthocyanins. The sulphured cold maceration wines were significantly greater than the control in visible colour, colour density, total anthocyanins, natural degree of ionisation, ionised anthocyanins and total phenolics. These results followed similar patterns with wine ageing, at six months these wines were still significantly greater in all the measurements apart from natural degree of ionisation. The results for the sulphured cold maceration wines indicates that SO₂ is acting as a solvent for the extraction of phenolic compounds including anthocyanins. The 50 mgL⁻¹ SO₂ cold maceration wine had similar colour and phenolic content to the 100 mgL⁻¹ SO₂ cold maceration wine at bottling, at six months the 50 mgL⁻¹ SO₂ cold maceration wine still retained a similar colour to the 100 mgL⁻¹ SO₂ cold maceration wine but had vastly reduced anthocyanin content. This indicates that for the grapes utilised in this study the most appropriate level of addition at cold maceration would be 50 mgL⁻¹ of SO₂. With grapes of differing phenolic content the level of addition required will vary.

Mestrado Vinifera EuroMaster - Instituto Superior de Agronomia
Vine performance, fruit and wine composition were investigated on field grown Pinot Noir grapevines subjected to a range of leaf removal and cluster thinning treatments. Both treatments were applied in three levels (25 %, 50 % and 100 % of leaves and clusters retained, respectively) at the phenological stage of pea-size. New emerging leaves were removed as they appeared. Veraison was delayed when leaf area to fruit weight ratio (LA/Y) dropped below a certain threshold (13 cm2 g-1). On the contrary a high LA/Y ratio (> 26 cm2g-1) did not show any difference on fruit colouration. No significant differences in single leaf photosynthetic rate were observed between the treatments, however, there was a trend that vines from the 50 % leaf retained treatment tended to exhibit highest values for stomatal conductance and photosynthesis. Defoliated vines compensated for a restricted leaf area by increasing individual leaf size of the remaining leaves.The results of the present study suggest that fruit is produced at the expense of vegetative growth. High crop levels resulted in a decrease of individual leaf size. Moreover, pruning weight and LA/Y ratio were positively correlated. Sugar accumulation in grape berries were shown to follow a saturation curve. An increase of the source to sink ratio up to a certain point was accompanied by an increase in sugar accumulation. If this threshold was exceeded, additional leaf area did not promote higher sugar accumulation, indicating that the vine might be sink-limited. The most severe defoliation treatment significantly reduced berry size and berry weight. No consistent pattern between titratable acidity, pH and YAN and leaf area to fruit weight ratio could be found. Wine tannin content and wine colour parameters were not affected by the treatments

Precision agriculture applied to vitiviniculture can bring several advantages to vitis crop production with positive effects on grape and wine quality. This study s objective was to put into practice precision vtiticulture, aiming to improve the administration and management of vinifera grapes. This research was carried out in a commercial vitis cropland with 2.4 ha located in Encruzilhada do Sul (RS) close to RS/BR km 471. In this area, sandy clay loam Palleudult soil prevails. Sampling for the purposes for soil characterisation was carried out in 0-0,2 m depth. The soil attributes determined were: pH, SOM, texture, Ca, Mg, Al, N, MO, K and P, and the plant attributes were: productivity, alcoholic potential and tartaric acid, using a 35 X 35m grid. Data were analyzed through geostatistics by interpolation technique of kriging. The data of yield and enological parameters were obtained by manual harvest in georeferenced points. Grape data were collected during the harvest in 2007 and 2008 seasons. Soil and plant data were submitted to statistical analysis and basic geostatistic. Spatial variability of soil chemical attributes in the area behaved in accordance with the nutrient evaluated. pH showed low variability, clay, Ca, Mg, and N, showed median variability while MO, P and K , a high variability was found, and for Al, very high. In 2007 harvest, tartaric acid and alcoholic potential showed low variability, number of bunches per plant and weight per bunch presented median variability while weight per plant showed high variability. In 2008 harvest, alcoholic potential showed low variability, while tartaric acid, number of bunches per plant, weight per plant, weight per bunch and number of gems showed median variability. The 35 X 35 m sampling grid has shown itself adequate for soil attributes excepting clay, which obtained a 30.06 m distance reach. In 2007 harvest, the alcoholic potential was the plant attribute that showed smaller reach n the area, 18.42 m. In 2008 harvest, all the plant attributes studied showed a below 35 m reach. The correlation analysis has revealed itself significant among nitrogen and alcoholic potential in 2007 harvest. In 2008 harvest, phosphorus and potassium showed significant correlation with alcoholic potential. The comparison of the demand for the application of fertilizer at varied rate, with application at steady rate of lime, phosphorus and potassium was based on the soil sampling. In the case of lime, there was a 700 kg saving of it with varied rate application, the same was not observed with phosphorus and potassium, which would increase the product quantity if implemented this way, this happens because the levels of them in the soil are appropriate. The adoption of precision viticulture can help the wine grower to know better and further the productive area, allowing to improve the quality and quantity of the vineyard.
O uso da agricultura de precisão aplicada à vitivinicultura pode trazer benefícios à cultura da vitis e à cadeia produtiva, com reflexos na qualidade da uva e do vinho. O objetivo deste estudo foi aplicar a vitivinicultura de precisão, visando aprimorar a gestão e o manejo de áreas produtivas de uvas viníferas. O estudo foi conduzido em um vinhedo comercial, numa área de 2,4 ha, destinado à fabricação de espumantes situada às margens da RS/BR km 471, em Encruzilhada do Sul-RS. Na área predominam solos classificados como Argissolo Vermelho distrófico. A amostragem para fins de caracterização do solo foram realizadas na profundidade de 0-0,2m. Os atributos de solo determinados foram: pH, SMP, Argila, Ca, Mg, Al, N, MO, K e P, e os atributos de planta foram produtividade, ácido tartárico e potencial alcoólico usando uma malha de amostragem de 35 X 35 m. Os dados foram analisados através da técnica de interpolação geoestatística por krigagem. Os dados de rendimento e parâmetros enológicos foram obtidos por colheita manual em pontos georreferenciados. Os dados de uva foram coletados durante a colheita nas safras 2007 e 2008. Os dados de solo e de planta foram submetidos à analise estatística básica e geoestatistica. A variabilidade espacial dos atributos químicos do solo na área comportou-se de forma distinta de acordo com o nutriente avaliado. O pH apresentou variabilidade baixa, a Argila, o Ca, o Mg, e o N, o apresentaram variabilidade média enquanto que a MO, o K e o P, a variabilidade encontrada foi alta, e para o Al muito alta. Na safra 2007, o ácido tartárico e o potencial alcoólico apresentaram variabilidade baixa, o número de cachos por planta e o peso por cacho apresentaram variabilidade média enquanto que o peso por planta apresentou variabilidade alta. Na safra 2008, o potencial alcoólico apresentou variabilidade baixa, enquanto que o ácido tartárico, o número de cachos por planta, o peso por planta, o peso por cacho e o número de gemas apresentaram variabilidade média. A malha de amostragem de 35X35m se mostrou adequado para os atributos de solo com exceção da argila, que obteve uma distância de alcance de 30,06m. Na safra 2007 o potencial alcoólico foi o atributo de planta que apresentou menor alcance na área, de 18,42 m. Na safra 2008, todos os atributos de planta estudados apresentaram alcance inferior a 35 m. A analise da correlação se revelou significativa entre o nitrogênio e o potencial alcoólico na safra 2007. Na safra 2008, o fósforo e o potássio mostraram correlação significativa com o potencial alcoólico. A comparação da demanda de aplicação de fertilizantes a taxa variada, com a aplicação à taxa fixa de calcário, fósforo e potássio foi feita com base na amostragem de solo. No caso do calcário observou-se uma economia de 700 kg do mesmo com aplicação a taxa variada, o mesmo não foi observado com o fósforo e com o potássio, que aumentaria a quantidade do produto caso fosse aplicado desta forma, isso se dá pelo fato dos teores dos mesmos no solo serem adequados. A adoção da vitivinicultura de precisão pode auxiliar o viticultor a conhecer melhor e mais detalhadamente a área produtiva, permitindo aprimorar a qualidade e quantidade do vinhedo.

An aero gas turbine combustor has to meet requirements for both high and low power condition operation. Within the requirements for low power conditions, lean-blow-out (LBO) and combustion efficiency are the basic ones. A pure prefilming air blast atomizer may have difficulty meeting combustion requirements under low power conditions, such as, idle LBO, idle combustion efficiency, etc. Use of a hybrid airblast atomizer may offer a solution for such problems. A hybrid airblast atomizer is a single fuel injection unit that has both pilot and main fuel circuits. A simplex nozzle is often used for pilot fuel circuit and an airblast atomizer of the swirl cup type may be used for the main fuel circuit. For the main fuel circuit, fuel is injected from a number of plain jet holes. The fuel jets are injected towards a venturi, with the help of swirling air from another air swirler, and the main fuel is airblasted and well mixed with both swirler airflows. For low power conditions, the pilot fuel nozzle (simplex nozzle) works alone. Not all of the swirler air will mix with pilot nozzle fuel spray. With appropriate pilot nozzle pressure drop and with some airblast function, the pilot fuel is well atomized and does not fully mix with the swirler air nor with primary hole air. Thus, the low power condition combustion efficiency is improved. The investigation reported in the present paper has concentrated on hybrid atomizer combustion performance under simulated low power conditions, when only the pilot nozzle is operating. The study consists of the following parts: • Pilot nozzle drop size measurement; • Numerical simulation of combustor flow field; • Atmospheric ignition test; • Simulated idle condition LBO test; • Low power condition combustion efficiency test. Results are reported, and future work is defined.

Abstract Thanks to their absence of play, absence of contact friction and possible monolithic fabrication, flexure pivots offer advantages over traditional bearings in small-scale, high accuracy applications and environments where lubrication and wear debris are proscribed. However, they typically present a so-called parasitic center shift that deteriorates their rotational guidance accuracy. Existing solutions addressing this issue have the drawbacks of reducing angular stroke, prohibiting planar design, or introducing overconstraints or underconstraints. This article introduces a new triple crossed flexure pivot called TRIVOT that has a reduced parasitic shift without overconstraints nor internal mobility while allowing either optimal stress distribution in the flexures or a planar design. The new architecture also makes it possible to place the center of rotation outside of the physical structure, which is not the case with traditional bearings. Based on finite element simulations, we show that the parasitic shift is reduced by one order of magnitude in comparison to the widely used crossed flexure pivot. We also derive and validate formulas for the rotational stiffness and angular stroke limit of the TRIVOT for given dimensions and material, which are valuable for its dimensioning towards practical applications. We expect this new pivot to become a competitive alternative to the crossed flexure pivot for applications where high accuracy and compactness are required.

Abstract In recent years the interest for new advanced thermodynamical gas turbine cycles has increased. One of the new designs is the evaporative gas turbine cycle. A lot of effort worldwide has been put into predicting the possible efficiency, pollutants, and dynamic behaviour of the evaporative gas turbine cycle, but all results so far have been affected by uncertain assumptions. Until now this cycle has not been demonstrated in a pilot plant. The purpose of this work has been to identify the potential of this cycle, by erecting a pilot plant at the Lund Institute of Technology. The project was financed on a 50/50 basis from the Swedish National Energy Administration and the industrial partners. Three different thermodynamical cycles have been tested in the pilot plant: the simple, the recuperative, and the evaporative cycles. The final pilot plant roughly consists of a 600 kW gas turbine, a hydraulic brake, a recuperator, a humidification tower, an economiser, and a flue gas condenser. All layout and functional analysis were made within the project. The pilot plant is, however, optimized neither for best efficiency nor for best emissions. It has only been built for demonstration purpose. It has been shown from the performance tests that the efficiency for the simple, recuperative, and evaporative cycles are 22, 27, and 35%, respectively, at rated power output. The NOx emissions were reduced by 90% to under 10 ppm, and the UHC and CO were not measurable when running the evaporative cycle at rated power output. The performance of the humidification tower was better than expected. The humidified air out from the humidification tower is always saturated. The pinch point, i.e. the temperature difference between the outcoming water from the humidification tower and the saturation temperature of the incoming air, is around 3°C. The water circuit was closed, i.e. there was no need for additional water, when the flue gases after the flue gas condenser reached a temperature of 35° C. The inhouse heat balance program, used for both cycle optimization and evaluation, has been verified. The evaporative gas turbine cycle has, when optimized, at least the same efficiency as the best combined cycle today, based on the same gas turbine. The evaporative cycle will also show very good performance when used in small scale power plants.

Abstract In recent years the interest for new advanced thermodynamical gas turbine cycles has increased. One of the new designs is the evaporative gas turbine cycle. A lot of effort worldwide has been put into predicting the possible efficiency, pollutants, and dynamic behaviour of the evaporative gas turbine cycle, but all results so far have been affected by uncertain assumptions. Until now this cycle has not been demonstrated in a pilot plant. The purpose of this work has been to identify the potential of this cycle, by erecting a pilot plant at the Lund Institute of Technology. The project was financed on a 50/50 basis from the Swedish National Energy Administration and the industrial partners. Three different thermodynamical cycles have been tested in the pilot plant: the simple, the recuperative, and the evaporative cycle. The final pilot plant roughly consists of a 600 kW gas turbine, a hydraulic brake, a recuperator, a humidification tower, an economiser, and a flue gas condenser. All layout and functional analysis were made within the project. The pilot plant is, however, optimized neither for best efficiency nor for best emissions, due to the choice of standard components out of economical reasons. It has only been built for demonstration purpose. Maximum simplicity, flexibility and safety have been the main emphasis in the design of the EvGT cycle. The flow mismatch that occurs between the compressor and the expander in evaporative cycles makes it hard to use a standard gas turbine unit. To be able to use a standard unit, an air bleed off system has been introduced. The water circuit can, at any time, be connected or disconnected from the humidification tower, thereby creating a possibility of controlling when humidification takes place or not by means of a water bypass past the humidification tower. Two starting sequences have been developed, one with the humidification process fully integrated from the beginning and one without. It has been shown that it is possible to reach full power output from the evaporative gas turbine cycle almost as fast as for the simple cycle. It has also been shown that the process is very stable when operated at various loads and during load transients. Furthermore, it is possible to start the power plant quickly from a remote place.

Studies being presented here have been done as initial part of wider research-and-developemnt (R&amp;D) programme, aiming to investigate preconditions and all instruments set necessary for environmental governance adequate integration into legally required societal (sustainable) development governance/planning framework and municipal governance practice. This have been done studying governance by its triple complementary dimensions of governance content, governance process and governance stakeholders and approaching municipality as a complex and interactive socio-ecological system. Accordingly, pilot investigations have been done as various thematical case studies in preselected model municipalities – six studies/pilot studies, based on the integrated case study research methodology, including and complementary applying document studies, interviews, surveys, observations and focus groups. Existing development planning and other instruments combined with municipal planning capacities are neither sufficient nor effective, and, since step-wise developmental process for sustainability principle integration seems to be not perspective as quite similar also with integration principle as for complex planning process and also as for thematical meaning of integrations of environmental governance into develoment one. Since environmental sector planning documents are not mandatory anymore at local municipalities, environmental sector planning process and content in the development planning practice is often limited, but few ad hoc available and used formal environmental planning instruments are neither sufficient or used just temporaly. There is to be seen necessity to develop innovative methodologies and tools as done during this research-and-development project.

Abstract In brownfields, controlling well integrity is critical in maintaining production and ensuring safety of the personnel and infrastructures. Equally important is optimizing and allocating production in wells by closely following wellhead upstream pressures (and temperatures). In the current situation, field crews have to move from well to well. This method is time consuming, exposes personnel to driving hazards and potentially dangerous areas. In addition, human reading of manual pressure gauges can result in large discrepancy in the reported values. Together with the low frequency of manual readings, this method does not allow for pro-active well intervention and can result in higher downtime in case of well tripping. Deploying remote monitoring with classical telemetry in fields with limited telecommunication infrastructure is costly and complex. Low Range Wide Area Network (LoRaWAN), a public wireless network technology developed in 2009, changes the situation. It enables low power compact battery sensors with up to 10 km radio range. This performance is sufficient to connect, in one go, most onshore wells without power nor connectivity. This paper describes a pilot project to evaluate the adequacy of this technology in ADNOC Onshore fields. The objective is to assess performance of LoRaWAN deployed Sensors along four metrics: deployment time, deployment cost, Base station radio coverage and data availability. The pilot uses a plug-in ATEX- certified Wireless Pressure and Temperature (P&T) sensors developed by the vendor SRETT, commercial LoRaWAN Base stations, and proprietary software to provide remote access to the data via cloud data storage and web based application. For this pilot, four Base stations were deployed in two giant oil fields collecting data from four well heads each equipped with two sensors (P&T). This combination allowed testing wireless link quality over eight radio paths, some with terrain obstacles between Sensors and Base stations. The complete system was fully tested and validated at the shop prior to field deployment. Performances during the deployment was evaluated, and Sensor behaviors were monitored over a three-month period. In the current environment, maintaining a high HSE standard on aging infrastructure must be made at a controlled cost. LoRaWAN IoT remote monitoring technology is cost effective and efficient to deploy. Once deployed, it will enable preventative safe detection of wells with potential issues, improved accuracy and understanding of production events and lead to a reduction of potential adverse situations thanks to an optimized intervention strategy.

The paper addresses the coordinated path planning of mobile agents with multiple goal positions and orientations in a plane. The targeted multi-robot system uses discrete locomotion ensuring uncertainty-free localization and mapping as well as simple and robust control. It is suitable for material-handling, reconfigurable-fixturing, and mobile-manipulation tasks in a flexible-manufacturing environment. Using its three leg, and matching pin-socket couplings with the base surface, each agent either stands fixed or strides along via “Swing and Dock” (SaD) locomotion. Each mounting pin can serve both as a connecting-locking device and as a pivot of a planar rotation. Previous work offered planning solutions only for the agents’ positions. In reality, the orientation in which the agent arrives at the goal is very important because neither robot workspaces nor workcell geometries have axial symmetry. Herein, we provide for the required orientational planning by labelling the agent’s legs to keep track of its rotation. Integer Linear Programming (ILP) is used to model the path planning problem in the so augmented configuration space. The mathematical formulations are implemented and tested using a GUROBI solver. Computational results display the effectiveness of the approach.

Orofacial pain associated with osteoarthritis (OA) in the temporomandibular joint (TMJ) is a significant clinical problem [1]. The pathophysiologic and cellular mediators that underlie the development of such chronic orofacial pain are not well understood, nor has a relationship to mechanical loading been defined. Several experimental models have been developed to examine causative factors in TMJ OA progression and joint pathology. Such models often involve intra-articular injections or surgical manipulation of tissue structures in order to alter joint kinematics and stability [2–6]. For example, severing of the discal attachments followed by anterior displacement of the disc has been employed in a rabbit model, while disc perforation and scraping of the condylar surface have been used in sheep models to induce OA symptoms [2,3]. A limitation of the above approaches is that they introduce artificial damage to the joint structures and do not approximate the clinical disorder of mechanically-induced TMJ OA. Therefore, the goal of this pilot study was to develop a novel model of TMJ OA via non-invasive and mechanically relevant methods that could produce behavioral hypersensitivity (mechanical allodynia) suggestive of pain symptoms and histological changes in the TMJ consistent with osteoarthritic pathology.

Genetic analysis and mapping in grapes has been difficult because of the long generation period and paucity of genetic markers. In the present work, chromosome linkage maps were developed with RAPD, RFLP and isozyme loci in interspecific hybrid cultivars, and RAPD markers were produced in a V. vinifera population. In three cultivars, there were 19 linkage groups as expected for a species with 38 somatic chromosomes. These maps were used to locate chromosome regions with linkages to important genes, including those influencing powdery mildew and botrytis bunch rot resistance; flower sex; and berry shape. In V. vinifera, the occurrence of specific markers was correlated with seedlessness, muscat flavor and fruit color. Polymorphic RAPD bands included single copy as well as repetitive DNA. Mapping procedures were improved by optimizing PCR parameters with grape DNA; by the development of an efficient DNA extraction protocol; and with the use of long (17- to 24-mer) primers which amplify more polymorphic loci per primer. DNA fingerprint analysis with RAPD markers indicated that vinifera cultivars could be separated readily with RAPD profiles. Pinot gris, thought to be a sort of Pinot noir, differed by 12 bands from Pinot noir. This suggests that while Pinot gris may be related to Pinot noir, it is not likely to be a clone. The techniques developed in this project are now being further refined to use marker-assisted selection in breeding programs for the early selection of elite seedlings. Furthermore, the stage has been set for future attempts to clone genes from grapes based upon map locations.

Grapevine is perhaps the most widely grown fruit crop. To understand the genetic make-up so as to improve the yield and quality of grapes and grape products, researchers in Europe have recently sequenced the genomes of Pinot noir and its inbred. As expected, function of many grape genes is unknown. Functional genomics studies have become the major focus of grape researchers and breeders. Current genetic approaches for gene function studies include mutagenesis, crossing and genetic transformation. However, these approaches are difficult to apply to grapes and takes long periods of time to accomplish. It is thus imperative to seek new ways for grape functional genomics studies. Virus-induced gene silencing (VIGS) offers an attractive alternative for this purpose and has proven highly effective in several herbaceous plant species including tomato, tobacco and barley. VIGS offers several advantages over existing functional genomics approaches. First, it does not require transformation to silence a plant gene target. Instead, it induces silencing of a plant gene through infection with a virus that contains the target gene sequence, which can be accomplished within a few weeks. Second, different plant genes can be readily inserted into the viral genome via molecular cloning and functions of a large number of genes can be identified within a short period of time. Our long-term goal of this research is to develop VIGS-based tools for grapevine functional genomics, made of the genomes of Grapevine virus A (GVA) from Israel and Grapevine rupestris stem pitting-associated virus (GRSPaV) from Canada. GVA and GRSPaV are members of the Flexiviridae. Both viruses have single-stranded, positive sense RNA genomes, which makes them easy to manipulate genetically and excellent candidates as VIGS vectors. In our three years research, several major breakthroughs have been made by the research groups involved in this project. We have engineered a cDNA clone of GVA into a binary vector that is infectious upon delivery into plantlets of micropropagated Vitis viniferacv. Prime. We further developed the GVA into an expression vector that successfully capable to silence endogenous genes. We also were able to assemble an infectious full-length cDNA clones of GRSPaV. In the following sections Achievements and Detailed description of the research activities, we are presenting the outcome and results of this research in details.

The study was not designed to undertake an evaluation of the success or failure of reform. Nor was it specifically about the desirability or defects of the policy reform choices. It took these reform choices and the policy context as a given. It is important to note that the Delhi reforms had its share of criticisms (Kumar, 2016; Rampal, 2016). However, our goal was not to comment on whether these were the “right” reforms or have their appropriateness measured in terms of their technical capability. This study sought to understand the pathways through which policy formulations, designed and promoted by committed leaders (the sound and functional head of the flailing state), transmit their ideas and how these are understood, resisted, and adopted on the ground. In essence, this is a study that sought to illuminate the multifaceted challenges of introducing change and transition in low-capacity settings. Its focus was on documenting the process of implementing reforms and the dynamics of resistance, distortion, and acceptance of reform efforts on the ground. The provocative claim that this report makes is that the success and failure, and eventual institutionalisation, of reforms depend fundamentally on how the frontline of the system understands, interprets, and adapts to reform efforts. This, we shall argue, holds the key to upending the status quo of “pilot” burial grounds that characterise many education reform efforts in India. Reforms are never implemented in a vacuum. They inevitably intersect with the belief systems, cultures, values, and norms that shape the education ecosystem. The dynamics of this interaction, the frictions it creates, and reformers’ ability to negotiate these frictions are what ultimately shape outcomes. In the ultimate analysis, we argue that reforming deeply entrenched education systems (and, more broadly, public service delivery systems) is not merely a matter of political will and technical solutions (although both are critical). It is about identifying the points of reform friction in the ecosystem and experimenting with different ways of negotiating these. The narrative presented here does not have any clear answers for what needs to be done right. Instead, it seeks to make visible the intricacies and potential levers of change that tend to be ignored in the rush to “evaluate” reforms and declare success and failure. Moving beyond success to understand the dynamics of change and resistance is the primary contribution of this study.

Background Lung cancer is the number one cause of cancer death worldwide.(1) It is the fifth most commonly diagnosed cancer in Australia (12,741 cases diagnosed in 2018) and the leading cause of cancer death.(2) The number of years of potential life lost to lung cancer in Australia is estimated to be 58,450, similar to that of colorectal and breast cancer combined.(3) While tobacco control strategies are most effective for disease prevention in the general population, early detection via low dose computed tomography (LDCT) screening in high-risk populations is a viable option for detecting asymptomatic disease in current (13%) and former (24%) Australian smokers.(4) The purpose of this Evidence Check review is to identify and analyse existing and emerging evidence for LDCT lung cancer screening in high-risk individuals to guide future program and policy planning. Evidence Check questions This review aimed to address the following questions: 1. What is the evidence for the effectiveness of lung cancer screening for higher-risk individuals? 2. What is the evidence of potential harms from lung cancer screening for higher-risk individuals? 3. What are the main components of recent major lung cancer screening programs or trials? 4. What is the cost-effectiveness of lung cancer screening programs (include studies of cost–utility)? Summary of methods The authors searched the peer-reviewed literature across three databases (MEDLINE, PsycINFO and Embase) for existing systematic reviews and original studies published between 1 January 2009 and 8 August 2019. Fifteen systematic reviews (of which 8 were contemporary) and 64 original publications met the inclusion criteria set across the four questions. Key findings Question 1: What is the evidence for the effectiveness of lung cancer screening for higher-risk individuals? There is sufficient evidence from systematic reviews and meta-analyses of combined (pooled) data from screening trials (of high-risk individuals) to indicate that LDCT examination is clinically effective in reducing lung cancer mortality. In 2011, the landmark National Lung Cancer Screening Trial (NLST, a large-scale randomised controlled trial [RCT] conducted in the US) reported a 20% (95% CI 6.8% – 26.7%; P=0.004) relative reduction in mortality among long-term heavy smokers over three rounds of annual screening. High-risk eligibility criteria was defined as people aged 55–74 years with a smoking history of ≥30 pack-years (years in which a smoker has consumed 20-plus cigarettes each day) and, for former smokers, ≥30 pack-years and have quit within the past 15 years.(5) All-cause mortality was reduced by 6.7% (95% CI, 1.2% – 13.6%; P=0.02). Initial data from the second landmark RCT, the NEderlands-Leuvens Longkanker Screenings ONderzoek (known as the NELSON trial), have found an even greater reduction of 26% (95% CI, 9% – 41%) in lung cancer mortality, with full trial results yet to be published.(6, 7) Pooled analyses, including several smaller-scale European LDCT screening trials insufficiently powered in their own right, collectively demonstrate a statistically significant reduction in lung cancer mortality (RR 0.82, 95% CI 0.73–0.91).(8) Despite the reduction in all-cause mortality found in the NLST, pooled analyses of seven trials found no statistically significant difference in all-cause mortality (RR 0.95, 95% CI 0.90–1.00).(8) However, cancer-specific mortality is currently the most relevant outcome in cancer screening trials. These seven trials demonstrated a significantly greater proportion of early stage cancers in LDCT groups compared with controls (RR 2.08, 95% CI 1.43–3.03). Thus, when considering results across mortality outcomes and early stage cancers diagnosed, LDCT screening is considered to be clinically effective. Question 2: What is the evidence of potential harms from lung cancer screening for higher-risk individuals? The harms of LDCT lung cancer screening include false positive tests and the consequences of unnecessary invasive follow-up procedures for conditions that are eventually diagnosed as benign. While LDCT screening leads to an increased frequency of invasive procedures, it does not result in greater mortality soon after an invasive procedure (in trial settings when compared with the control arm).(8) Overdiagnosis, exposure to radiation, psychological distress and an impact on quality of life are other known harms. Systematic review evidence indicates the benefits of LDCT screening are likely to outweigh the harms. The potential harms are likely to be reduced as refinements are made to LDCT screening protocols through: i) the application of risk predication models (e.g. the PLCOm2012), which enable a more accurate selection of the high-risk population through the use of specific criteria (beyond age and smoking history); ii) the use of nodule management algorithms (e.g. Lung-RADS, PanCan), which assist in the diagnostic evaluation of screen-detected nodules and cancers (e.g. more precise volumetric assessment of nodules); and, iii) more judicious selection of patients for invasive procedures. Recent evidence suggests a positive LDCT result may transiently increase psychological distress but does not have long-term adverse effects on psychological distress or health-related quality of life (HRQoL). With regards to smoking cessation, there is no evidence to suggest screening participation invokes a false sense of assurance in smokers, nor a reduction in motivation to quit. The NELSON and Danish trials found no difference in smoking cessation rates between LDCT screening and control groups. Higher net cessation rates, compared with general population, suggest those who participate in screening trials may already be motivated to quit. Question 3: What are the main components of recent major lung cancer screening programs or trials? There are no systematic reviews that capture the main components of recent major lung cancer screening trials and programs. We extracted evidence from original studies and clinical guidance documents and organised this into key groups to form a concise set of components for potential implementation of a national lung cancer screening program in Australia: 1. Identifying the high-risk population: recruitment, eligibility, selection and referral 2. Educating the public, people at high risk and healthcare providers; this includes creating awareness of lung cancer, the benefits and harms of LDCT screening, and shared decision-making 3. Components necessary for health services to deliver a screening program: a. Planning phase: e.g. human resources to coordinate the program, electronic data systems that integrate medical records information and link to an established national registry b. Implementation phase: e.g. human and technological resources required to conduct LDCT examinations, interpretation of reports and communication of results to participants c. Monitoring and evaluation phase: e.g. monitoring outcomes across patients, radiological reporting, compliance with established standards and a quality assurance program 4. Data reporting and research, e.g. audit and feedback to multidisciplinary teams, reporting outcomes to enhance international research into LDCT screening 5. Incorporation of smoking cessation interventions, e.g. specific programs designed for LDCT screening or referral to existing community or hospital-based services that deliver cessation interventions. Most original studies are single-institution evaluations that contain descriptive data about the processes required to establish and implement a high-risk population-based screening program. Across all studies there is a consistent message as to the challenges and complexities of establishing LDCT screening programs to attract people at high risk who will receive the greatest benefits from participation. With regards to smoking cessation, evidence from one systematic review indicates the optimal strategy for incorporating smoking cessation interventions into a LDCT screening program is unclear. There is widespread agreement that LDCT screening attendance presents a ‘teachable moment’ for cessation advice, especially among those people who receive a positive scan result. Smoking cessation is an area of significant research investment; for instance, eight US-based clinical trials are now underway that aim to address how best to design and deliver cessation programs within large-scale LDCT screening programs.(9) Question 4: What is the cost-effectiveness of lung cancer screening programs (include studies of cost–utility)? Assessing the value or cost-effectiveness of LDCT screening involves a complex interplay of factors including data on effectiveness and costs, and institutional context. A key input is data about the effectiveness of potential and current screening programs with respect to case detection, and the likely outcomes of treating those cases sooner (in the presence of LDCT screening) as opposed to later (in the absence of LDCT screening). Evidence about the cost-effectiveness of LDCT screening programs has been summarised in two systematic reviews. We identified a further 13 studies—five modelling studies, one discrete choice experiment and seven articles—that used a variety of methods to assess cost-effectiveness. Three modelling studies indicated LDCT screening was cost-effective in the settings of the US and Europe. Two studies—one from Australia and one from New Zealand—reported LDCT screening would not be cost-effective using NLST-like protocols. We anticipate that, following the full publication of the NELSON trial, cost-effectiveness studies will likely be updated with new data that reduce uncertainty about factors that influence modelling outcomes, including the findings of indeterminate nodules. Gaps in the evidence There is a large and accessible body of evidence as to the effectiveness (Q1) and harms (Q2) of LDCT screening for lung cancer. Nevertheless, there are significant gaps in the evidence about the program components that are required to implement an effective LDCT screening program (Q3). Questions about LDCT screening acceptability and feasibility were not explicitly included in the scope. However, as the evidence is based primarily on US programs and UK pilot studies, the relevance to the local setting requires careful consideration. The Queensland Lung Cancer Screening Study provides feasibility data about clinical aspects of LDCT screening but little about program design. The International Lung Screening Trial is still in the recruitment phase and findings are not yet available for inclusion in this Evidence Check. The Australian Population Based Screening Framework was developed to “inform decision-makers on the key issues to be considered when assessing potential screening programs in Australia”.(10) As the Framework is specific to population-based, rather than high-risk, screening programs, there is a lack of clarity about transferability of criteria. However, the Framework criteria do stipulate that a screening program must be acceptable to “important subgroups such as target participants who are from culturally and linguistically diverse backgrounds, Aboriginal and Torres Strait Islander people, people from disadvantaged groups and people with a disability”.(10) An extensive search of the literature highlighted that there is very little information about the acceptability of LDCT screening to these population groups in Australia. Yet they are part of the high-risk population.(10) There are also considerable gaps in the evidence about the cost-effectiveness of LDCT screening in different settings, including Australia. The evidence base in this area is rapidly evolving and is likely to include new data from the NELSON trial and incorporate data about the costs of targeted- and immuno-therapies as these treatments become more widely available in Australia.